TWM464150U - Microcapsule dressing containing stem cell conditioned medium composition liquid - Google Patents

Description

Microcapsule dressing with stem cell conditioned medium combination solution

The present invention relates to a microcapsule dressing having a stem cell conditioned medium combination solution, in particular to a dressing layer, a release paper and a cover layer, and the dressing layer comprises a matrix and a plurality of microcapsules distributed in the matrix; The microcapsules comprise a microcapsule dressing of a conditioned medium combination solution of Wharton's jelly mesenchymal stem cells (WJ-MSC) and human foreskin fibroblast cells.

Skin cell regeneration is associated with skin wound repair and skin maintenance. Regardless of the dressing suitable for wound repair or skin care (ie, a commercially available facial mask), the structure includes at least a cover layer, a dressing layer and a release paper; the difference lies in the material and use of the cover layer and the dressing layer; For the purpose of repair, the material of the cover of the conventional skin wound dressing comprises a polymer film, a tarpaulin, a non-woven fabric, a reticular fiber cloth, a nanofiber film or a polymer (Polymeric foam), and the cover layer is available for air and moisture. Pass, prevent bacteria from entering and prevent exudation of tissue fluid. The substrate which is conventionally used as a material for skin wound coverings includes: gel or hydrogel, and water film (Hydrocollo). Id) or alginate (Alginate), etc.; the matrix can absorb tissue fluid secreted by skin wounds and avoid sticking to skin wounds. However, as a skin care application, the cover layer and the dressing layer may be a material of one of paper, non-woven fabric, and biofiber which are conventionally used as a mask; the cover layer can reduce the drying speed of the dressing and increase the user's dressing. The absorption of the inner care product component, and the dressing layer is applied to the skin care component.

Generally, the commercially available skin wound dressings are effectively labeled as waterproofing and isolating foreign matter, and are easy to remove non-stick wounds. Medical grade skin wound dressings promote wound healing by improving the materials used or by adding certain ingredients such as collagen to promote cell proliferation or silver ions to increase bactericidal function. Skin care dressings add skin care ingredients to promote skin cell regeneration, ultimately achieving anti-wrinkle and moisturizing effects. The materials of the conventional cover layer and the dressing layer are as described above, and the researcher can achieve the effect of skin wound repair or skin care by the choice of materials and/or the addition of additional ingredients, but the effect is still limited. For this reason, the research and application of skin cell regeneration has its industrial applicability.

In addition, the current method is limited to simply applying the additive on the dressing, but the disadvantage is that after a period of time, it may cause a chemical reaction or deterioration between the various additives, that is, the effect of losing the additive, so it is necessary Looking for a solution; therefore, looking for a microcapsule dressing that promotes skin cell regeneration, and using a combination of different covering layers and dressing layers to achieve general, orthopedic, surgical skin wound repair or skin care can not achieve the promotion of the skin The effect of cell regeneration is the direction that researchers need to work hard.

Nowadays, the creator is still in the practical implementation of the existing dressings for skin care and skin wound repair. It is a tireless spirit, and with its rich professional knowledge and years of practical experience. Accompanied by the improvement, and based on this research and creation of this creation.

The main purpose of the present invention is to provide a microcapsule dressing having a stem cell conditioned medium composition solution, wherein the microcapsule dressing contains a conditioned medium combination solution of Wharton gel mesenchymal stem cells and human foreskin fibroblasts. The ability to promote skin cell regeneration for skin care and skin wound repair.

In order to achieve the above-mentioned object, the present inventors propose a microcapsule dressing having a stem cell conditioned medium composition solution, comprising: a dressing layer comprising a first surface and a pair of second surfaces corresponding to the first surface, a parallel stack a release paper laid on the first surface and a cover layer laminated on the second surface; the first surface is directly adhered to the skin of the user, and the dressing layer comprises a matrix and a plurality of microcapsules distributed in the matrix; wherein the microcapsules are hollow and form an accommodating space, and an appropriate amount of a conditioned medium composition containing cell culture can be disposed in the accommodating space. To promote skin cell regeneration; in addition, the release paper is arranged to prevent the microcapsule dressing from being contaminated under unused conditions; and the cover layer is disposed to prevent the microcapsule dressing from being contaminated under unused conditions.

In an embodiment of the present invention, a pressure sensitive adhesive layer may be further disposed between the cover layer and the dressing layer to increase the adhesion between the skin wound and the microcapsule dressing; wherein, the cover layer may be, for example, It is one of a polymer film, a tarpaulin, a non-woven fabric, a reticular fiber cloth, a nanofiber membrane or a polymer foam for allowing air and moisture to pass through, preventing bacteria from entering and preventing exudation of tissue fluid; further, wherein the matrix For example, it may be a material of a conventional skin wound covering, including one of a gel, a water gel, a water film or a seaweed gel, for adsorbing tissue fluid secreted by a skin wound, for coating a microcapsule and avoiding a sticky wound; The choice of material is used for the repair of skin wounds.

In an embodiment of the present invention, the cover layer may be, for example, one of paper, non-woven fabric, and biofiber to reduce the drying speed of the microcapsule dressing and increase the absorption of the effective substance in the microcapsule dressing by the user; Furthermore, the matrix may be, for example, one of paper, non-woven fabric or biofiber for coating the microcapsules; for the skin care by selection of the above materials.

In an embodiment of the present invention, wherein the microcapsules are composed of a low temperature dissolving wax, and the melting point of the low temperature dissolving wax is between 35 ° C and 45 ° C.

In an embodiment of the present invention, the conditioned medium for the cell culture comprises a conditioned medium of Wharton's mesenchymal stem cell culture and a conditioned medium for culture of human foreskin fibroblasts; and further, the conditioned medium for the cell culture The form may be, for example, one of a stock solution, a concentrate or a powder.

In an embodiment of the present invention, the conditioned medium composition solution for cell culture in the form of a concentrate may further add glycerin, vitamin C glycoside, human oligopeptide-1, human oligopeptide-3, human oligopeptide- 6 or one of pure water.

The conditioned medium contained in the microcapsule dressing can promote skin cell regeneration; and the microcapsule dressing can be applied to skin care and skin wound repair by selecting the cover layer and the material of the dressing layer to increase the Industrial utilization.

(1) ‧ ‧ cover layer

(2) ‧ ‧ dressing layer

(21) ‧‧‧ first surface

(22) ‧‧‧second surface

(23) ‧‧‧Matrix

(24)‧‧‧microcapsules

(241)‧‧‧Low-temperature dissolving wax

(242) ‧‧‧ accommodating space

(243) ‧‧‧Conditional medium combination solution

(3) ‧‧‧ release paper

The first figure: an exploded view of the preferred embodiment of the present creation

Second: Schematic diagram of the microcapsules of the preferred embodiment of the present invention

The purpose of this creation and its structural and functional advantages will be explained in accordance with the structure shown in the following figure, in conjunction with specific examples, so that the review committee can have a deeper and more specific understanding of the creation.

First, please refer to the first and second figures at the same time, which are respectively an exploded view of the preferred embodiment of the present invention and a schematic structural view of the microcapsule of the preferred embodiment. The present invention relates to a microcapsule dressing having a stem cell conditioned medium combination solution comprising at least: a dressing layer (2) comprising a first surface (21) and a second surface (22) corresponding to the first surface (21); a dressing layer (2) comprising a matrix (23) and a plurality of microcapsules (24) distributed in the matrix (23); the microcapsules (24) are hollow and form an accommodating space (242), and are The above-mentioned accommodation space (242) is provided with an appropriate amount of a conditioned medium composition solution (243) containing cell culture for promoting skin cell regeneration; a release paper (3) is placed on the first surface (21) in parallel. The microcapsule dressing can be prevented from being contaminated under the unused condition; and a cover layer (1) is placed on the second surface (22) in parallel, which can prevent the microcapsule dressing from being subjected to the unused condition. Pollution.

In actual use, after the release paper (3) is removed, the first surface (21) is directly adhered to the skin of the user, and the microcapsule (24) can be low temperature. The dissolved wax (241) is composed, and the melting point of the low-temperature dissolving wax (241) is between 35 ° C and 45 ° C, which facilitates the release of its contents by body temperature during use; and the conditioned medium for cell culture includes Watson's Conditioned medium for gel mesenchymal stem cell culture and conditioned medium for culture of human foreskin fibroblasts; the literature indicates that the conditioned medium of Wharton's mesenchymal stem cell culture contains various human growth factors, including epidermal growth factor (EGF) and alkali. Fibroblast growth factor (b-FGF) and skin stem cell growth factor (SCF) are involved in skin cell regeneration; however, the relative content of basic fibroblast growth factor in conditioned medium of Wharton's mesenchymal stem cell culture is higher. Less, solid can be supplemented by the addition of conditioned medium cultured with human foreskin fibroblasts containing more basic fibroblast growth factor; epidermal growth factor binds to the epidermal growth factor receptor in the basal cell membrane of the skin epidermis ( EGFR) accelerates epidermal cell division and is the main driving element of collagen synthesis, which can effectively produce and strengthen tissue fibers. Vitamins, rapidly improve skin metabolism, help skin growth, metabolism in shallow, middle and deep tissues, synergize with elastic fibers and collagen to achieve anti-wrinkle and skin rejuvenation; and basic fibroblast growth factor assists angiogenesis (angiogenesis) ), the function of wound healing, and at the same time has the function of fibroblast, which stimulates fibroblasts to activate fibroblasts; in addition, skin stem cell growth factor mainly acts to induce basal stem cell proliferation The conditioned medium for cell culture may be added to the conditioned medium composition (243) containing the cell culture in the form of one of a stock solution, a concentrate or a powder, and the ratio of the addition thereof is adjusted.

Furthermore, a conditioned medium composition solution for cell culture in the form of a concentrate (2 In 43), a component of one of glycerin, vitamin C glycoside, human oligopeptide-1, human oligopeptide-3, human oligopeptide-6 or pure water may be further added; wherein glycerin may increase skin moisturization Vitamin C glycoside vitamin C is followed by an inducer of two glucose molecules, which not only has the strong reducing power and antioxidant effect of vitamin C, but also has better stability; and human oligopeptide-1 ( The addition of human oligopeptide-3 (commonly known as basic fibroblast growth factor) and human oligopeptide-6 (commonly known as skin stem cell growth factor) can be supplemented and enhanced for a single component. Its effect.

In addition, the microcapsules (24) can be placed in a coating to facilitate application to the substrate (23), and the selection of the material of the coating layer (2) and the covering layer (1) can increase the industrial utilization of the microcapsule dressing. Sex.

When used as a repair for skin wounds, the cover layer (1) may be a material of a cover layer (1) of a conventional skin wound dressing, including a polymer film, a tarpaulin, a non-woven fabric, a reticular fiber cloth, a nanofiber film or One of the polymer foams; here, the cover layer (1) is used for the passage of air and moisture, prevents the entry of bacteria and prevents the exudation of tissue fluid for the repair of skin wounds. The substrate (23) may be a material of a conventional skin wound covering, including: one of gel or water gel, water film or seaweed gum; the matrix (23) can adsorb tissue fluid secreted by the skin wound, for microcapsules (24) Applying and avoiding adhering wounds; adding ingredients in the conditioned medium composition (243) containing cell culture by combining different dressing layers (2) and cover layers (1) for conventional skin wound dressings In proportion, this microcapsule dressing can be used for general, orthopedic or surgical skin wound repair. A pressure sensitive adhesive layer (not shown) may be further disposed between the cover layer (1) and the dressing layer (2). The pressure sensitive adhesive layer increases the tightness between the skin wound and the microcapsule dressing for the repair of the skin wound.

However, as a skin care dressing, the cover layer (1) and the dressing layer (2) may be a material of one of paper, non-woven fabric, and biofiber which are conventionally used as a skin care dressing (mask); 1) can reduce the drying speed of the microcapsule dressing and increase the absorption of the effective substance in the microcapsule dressing by the user; and the substrate (23) can be coated by the microcapsule (24); by combining different conventional skin care dressings The ratio of the material of the dressing layer (2) and the covering layer (1) to the composition of the conditioned medium combination solution (243) containing the cell culture can be used for skin care.

It can be seen from the above description that the present creation has the following advantages compared with the prior art:

1. The conditioned medium for cell culture used in the present invention contains various growth factors and is a cell source, which is close to the physiological state compared with the growth factors of general synthetic or genetic engineering.

2. The microcapsule dressing of the present invention can promote skin cell regeneration by the conditioned medium containing the cell culture, and the microcapsule facilitates the preservation of the contained components.

3. The microcapsule dressing of the present invention can increase the industrial applicability of the microcapsule dressing by selecting the material of the dressing layer and the covering layer.

In summary, the microcapsule dressing of the present invention can achieve the intended use effect by the above disclosed embodiments, and the creation has not been disclosed before the application, and has fully complied with the requirements and requirements of the patent law. . If you apply for a new type of patent in accordance with the law, you are welcome to review it and grant a patent.

However, the illustrations and descriptions disclosed above are only preferred embodiments of the present invention, and are not intended to limit the scope of protection of the present invention; those who are familiar with the skill are otherwise characterized by the scope of the creation. Equivalent changes or modifications shall be considered as not departing from the design of this creation.

(1) ‧ ‧ cover layer

(2) ‧ ‧ dressing layer

(21) ‧‧‧ first surface

(22) ‧‧‧second surface

(23) ‧‧‧Matrix

(24)‧‧‧microcapsules

(3) ‧‧‧ release paper

Claims (10)

A microcapsule dressing having a stem cell conditioned medium combination solution, comprising at least: a dressing layer comprising a first surface and a pair of second surfaces that should be the first surface, and the first surface is directly adhered to the use when in use The skin layer comprises a matrix and a plurality of microcapsules distributed in the matrix; wherein the microcapsules are hollow and form an accommodating space, and an appropriate amount of cell culture is set in the accommodating space. a conditioned medium composition solution for promoting skin cell regeneration; a release paper, placed in parallel on the first surface to prevent contamination of the microcapsule dressing under unused conditions; and a cover layer, parallel stack Laying on the second surface prevents the microcapsule dressing from being contaminated under unused conditions. The microcapsule dressing according to claim 1, wherein a pressure sensitive adhesive layer is further disposed between the covering layer and the dressing layer, and the pressure sensitive adhesive layer increases the adhesion between the skin wound and the microcapsule dressing. For the repair of skin wounds. The microcapsule dressing according to claim 2, wherein the covering layer is selected from the group consisting of a polymer film, a waterproof cloth, a non-woven fabric, a reticular fiber cloth, a nanofiber film or a polymer foam. Air and moisture pass through, prevent bacteria from entering and prevent exudation of tissue fluid for repair of skin wounds. The microcapsule dressing of claim 2, wherein the matrix is selected from the group consisting of a material of a conventional skin wound covering, including one of a gel or a water gel, a water film or a seaweed; the matrix is a skin wound The secreted tissue fluid is applied to the microcapsules to avoid adhesion to the wound for repair of the skin wound. The microcapsule dressing of claim 1, wherein the covering layer is selected from the group consisting of paper, non-woven fabric, and biofiber; the covering layer reduces the drying speed of the microcapsule dressing and increases the user's Absorption of active substances in capsule dressings for skin care. The microcapsule dressing of claim 1, wherein the matrix is selected from the group consisting of paper, nonwoven or biofiber; the matrix is for microcapsule coating for skin care. The microcapsule dressing of claim 1, wherein the microcapsules are composed of a low temperature dissolving wax, and the melting point of the low temperature dissolving wax is between 35 ° C and 45 ° C. The microcapsule dressing of claim 1, wherein the conditioned medium for cell culture comprises a conditioned medium of Watson's gel mesenchymal stem cell culture and a conditioned medium for human foreskin fibroblast culture. The microcapsule dressing of claim 8, wherein the conditioned medium of the cell culture is in the form of one of a stock solution, a concentrate or a powder. The microcapsule dressing according to claim 9, wherein the conditioned medium composition for cell culture in the form of a concentrate may further add glycerin, vitamin C glycoside, human oligopeptide-1, human oligopeptide-3, One of human oligopeptide-6 or pure water.