TWM461988U - Sterile cultivation box - Google Patents

Description

Sterile culture box

The present invention relates to a sterile culture box, and more particularly to a sterile culture box for use in aseptic culture applications such as plants.

At present, a sterile culture box commonly used for aseptic culture such as plants, bacteria or cells, tissues, etc., as shown in Fig. 6 and Fig. 7, the sterile culture boxes 40, 50 mainly comprise a box 41, 51 and a lid. 42, 52, wherein the conventional aseptic culture box 40 shown in FIG. 6 is such that the ring groove 421 at the bottom of the lid 42 is combined with the top circumference 411 of the box body 41 in a full-week manner, and the other shown in FIG. The conventional aseptic culture cartridge 50 is configured such that the lid 52 is screwed to the top of the casing 41 so that the lid 52 is detachably assembled to the casing 51.

The conventional aseptic culture cassette has a gas exchanged inside and outside the box, wherein a perforation is arranged in the outer side wall of the box 42 of the conventional aseptic culture box 40 shown in FIG. 6, and a gas permeable membrane is attached to the outer side of the perforation. The conventional aseptic culture cartridge 50 shown in Fig. 7 is provided with a perforation in the center of the top of the lid 52, and a gas permeable membrane 53 is attached to the outside of the perforation. The gas permeable membrane 53 is externally provided with a film on the outside of the perforation.

The aforementioned aseptic culture cassette is applied to, for example, plant seedlings, bacteria, ... waiting for culture for boxed aseptic culture, which is filled with a sterile medium and a seedling in a box, and then the lid is closed on the box, and vacuumed. After the sterilization process such as equipment and ozone, it is brought to a near-sterile state, and the plant seedlings are protected by a sterile culture box for storage or transportation. The gas permeable membrane provided on the box or the lid of the sterile culture box is used for gas exchange, and the invasion of external mold or foreign matter is blocked to facilitate the growth of the culture in a sterile culture box.

However, the creation of the aforementioned sterile culture box can provide training to be cultivated. Aseptic culture container of the material, however, the gas permeable membranes in the conventional aseptic culture cassettes adopt an exposed structure, so that the gas-permeable membranes are easily destroyed when the conventional sterile culture boxes are used, and the sterile culture boxes are stacked. Easy to be covered and other issues.

In addition, conventional sterile culture boxes are extinguished in a vacuum apparatus. During the treatment of the bacteria, the combined parts such as the engagement or the screwing between the casing and the cover are easily deformed, so that the engagement or the screw joint portion cannot be completely sealed and has a gap, and it is difficult to effectively block the invasion of external mold or foreign matter.

The main purpose of this creation is to provide a sterile culture box, which is designed to solve the problem that the gas permeable membrane of the existing sterile culture box is arranged on the top of the lid or in the side wall of the box, which is easily destroyed during use, and the aseptic culture box is stacked. Easy to be covered and other issues.

For the purpose of achieving the foregoing disclosure, the aseptic culture box proposed by the present invention comprises: a box body including a box mouth; a box cover, a detachable group cover at the box mouth of the box body, the lid and the box Forming a loop between the bodies and communicating through the mouth of the box to the gas permeable gap inside and outside the sterile culture box; A breathable loop is filled in the air permeable gap between the casing and the lid.

It is created by the aforementioned aseptic culture box, which is mainly formed by combining the lid and the box body, forming a loop shape between the two, and connecting the gas permeable gap inside and outside the sterile culture box through the mouth of the box, and loading in the annular gas permeable gap. The venting ring is arranged to provide the function of gas exchange inside and outside the box when the aseptic culture box is applied to plants and the like, and the gas permeable ring is hidden in the annular gas permeable gap between the lid and the box body by the venting ring system to make the gas permeable ring The ring is not easily damaged or obscured by foreign objects; moreover, even if the sterile culture box is sterilized in a vacuum apparatus, the joint between the box and the lid is slightly deformed, so that the air gap is changed, but The venting ring is installed in the air venting gap to filter, and still maintains its function of effectively blocking external mold or foreign matter.

The artificial aseptic culture box can further form a plurality of buckle protrusions on the upper part of the peripheral wall of the box body, and a plurality of buckle hooks are formed around the bottom of the box cover, and each buckle hook can respectively be buckled to the buckle protrusion of the box body Thereby, the lid set on the box body has the function of detachable group, has the function of facilitating opening of the lid from the box body, and is convenient for controlling the size of the opening, and the sterile culture box can be repeatedly used for multiple times. In line with the concept of environmental protection.

The creation of the aseptic culture box can also use the top of the lid to form an upper curved surface with a lower circumference and a lower circumference. When the aseptic culture box is not stacked, the external ambient light can pass through the curved surface by the curved surface of the lid. It can be evenly projected to all corners of the box, so that the plants and other organisms in the sterile culture box can receive light evenly.

The creation of the aseptic culture box can further utilize the bottom surface of the box body as a plane, and the bottom surface of the box body is distributed with a plurality of positioning portions, and the top of the lid is formed. a curved surface having a middle portion and a lower circumference, wherein the top surface of the cover is distributed with a plurality of upper positioning portions, and the upper positioning portions respectively correspond to the lower positioning portions for utilizing the upper layer box when the aseptic culture box is stacked up and down The lower positioning portion of the bottom surface is fixedly coupled with the upper positioning portion of the top surface of the lower cover, and the distance between the upper portion and the lower cover is larger, and the distance between the inner circumference and the lower cover is larger. Small, in order to facilitate the ambient light can be projected through the top of the lid, providing the light required by the organisms such as plants cultivated in the sterile culture box, and the outer ambient light can be evenly passed through the curved surface by the curved surface of the lid. Projected to all corners of the box to allow the plants and other organisms in the sterile culture box to receive light evenly.

The creation of the sterile culture box can further utilize the box and the box The cover is made of a transparent or translucent plastic component, and the lower part of the peripheral wall of the casing is provided with a plurality of spaced-apart marking lines for the user to place the reference reference of the position of the culture to be placed in the casing.

The creation of the sterile culture box can further make the box and the lid It is a corresponding polygon, and the box body and the lid respectively form an outer arc angle at each corner thereof to have better strength, and is not easily deformed during vacuum sterilization.

1‧‧‧ sterile incubator

10‧‧‧Box

11‧‧‧The top edge

12‧‧‧Snap convex

121‧‧‧Extension

122‧‧‧Deduction Department

13‧‧‧ Positioning Department

131‧‧‧Lower positioning piece

14‧‧‧ marking line

20‧‧‧ lid

21‧‧‧Rings

22‧‧‧ Sockets

23‧‧‧ buckle

231‧‧‧Bend

232‧‧‧Hook

24‧‧‧Upper Positioning Department

241‧‧‧Upper positioning piece

25‧‧‧Arc surface

30‧‧‧ breathable ring

40‧‧‧Septic culture box

41‧‧‧Box

411‧‧‧Top periphery

42‧‧‧ lid

421‧‧‧ Ring groove

50‧‧‧ sterile incubator

51‧‧‧Box

52‧‧‧ lid

53‧‧‧ breathable membrane

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a perspective view showing a preferred embodiment of a sterile culture cartridge of the present invention.

Figure 2 is a perspective exploded view of the preferred embodiment of the sterile culture cartridge of Figure 1.

Figure 3 is a side cross-sectional view showing a preferred embodiment of the sterile culture cartridge of Figures 1 and 2.

Figure 4 is a top plan view showing a plurality of seedlings of the preferred embodiment of the sterile culture box shown in Figures 1 and 2.

Fig. 5 is a view showing the state of use of the upper and lower stacks of the preferred embodiment of the sterile culture cartridge shown in Figs. 1 and 2.

Figure 6 is a schematic cross-sectional view of a conventional sterile culture cartridge.

Figure 7 is a schematic cross-sectional view of another conventional sterile culture cartridge.

As shown in FIG. 1 and FIG. 2, a preferred embodiment of the present sterile culture box 1 is disclosed. As can be seen from the drawings, the composition of the sterile culture box includes a box body 10 and a lid 20 And a breathable loop 30.

As shown in FIG. 2 and FIG. 3, the casing 10 is made of a plastic material, and the casing 10 can also be formed by using a transparent or translucent plastic material according to the kind of the culture. The body 10 can be a circular or polygonal hollow body. In the preferred embodiment, the casing 10 is substantially a quadrilateral body, and the square corners of the quadrilateral box 10 form an outer arc angle, so that the casing 10 has a comparative Good strength, not easy to deform.

An accommodating space is formed in the casing 10. The accommodating space extends to the top of the casing 10 to form a box opening and the ring top edge 11 at the periphery of the box mouth, and the upper wall of the casing 10 is distributed below the ring top edge 11. In the preferred embodiment, the box body 10 is a quadrilateral body, and a middle portion of the side wall of the box body 10 is formed below the ring top edge 11 to form a latching protrusion 12, and the buckle body is formed. The convex portion 12 includes an extending portion 121 horizontally protruding from the outer peripheral surface of the side wall of the casing 10, and a fastening portion 122 formed at the end of the extending portion 121.

As shown in FIG. 2 and FIG. 3, the cover 20 is made of plastic material. The cover member 20 can also be formed by using a transparent or translucent plastic material depending on the kind of the culture to be cultured, and the cover 20 is detachably assembled to cover the mouth of the casing 10. The cover 20 can be a circular or polygonal hollow body and corresponds to the top shape of the casing 10. In the preferred embodiment, the casing 10 is substantially a quadrilateral body, and the four corners of the quadrilateral casing 10 The outer arcuate corner is formed at the crotch, so that the cover 20 is also formed into a substantially quadrangular body. The four corners of the quadrangular cover 20 form an outer arc angle, so that the cover 20 also has better strength and is not easily deformed.

As shown in FIG. 2 and FIG. 3, the cover 20 is at the bottom of the bottom thereof. A plurality of buckles 23 are formed, and the buckles 23 are respectively fastened to the buckle protrusions 12 of the casing 10. In the preferred embodiment, a middle portion of the buckles 23 forms a bent portion 231, and the buckles are formed. The end has a hook portion 232 for covering the cover 20 to the casing 10. The bottom of the cover 20 abuts against the extension 121 of the buckle protrusion 12, and each buckle 23 can be bent by the protrusion 231. The hook portion 232 can be bent downward and snapped onto the fastening portion 122 of the buckle protrusion 12, as shown in FIG. 2 and FIG. 3, and the bottom of the cover 20 forms an annular sleeve portion 21, A sleeve groove 22 is formed in the annular sleeve portion 21, and the sleeve groove 22 is slightly larger than the box body 10. The annular sleeve portion 21 has a groove top surface at the top of the sleeve groove 22, and the sleeve groove There are groove side walls on both sides of the inner periphery of the casing 22, and the cover 20 is sleeved on the outer side of the top edge 11 of the top of the casing 10 with the annular sleeve portion 21, and the bottom edge of the annular sleeve portion 21 abuts against the buckle protrusion 12 The extending portion 121 has a small gap between the side groove wall on both sides of the inner side of the sleeve groove 22 and the outer side of the inner edge of the ring top edge 11, and the top surface of the ring top edge 11 and the top surface of the groove of the nesting groove 22 have One connected inner and outer sides The gap The gap is assembled such that the gap between the assembly gap and the inner and outer sides of the inner casing forms a gas permeable gap through the mouth of the box and communicating the inside and outside of the casing 10.

As shown in FIG. 2 and FIG. 3, the air permeable ring 30 can be cotton. The wool material of the quality or other fibers is made into a gas-permeable annular ring body and has a filtering function for blocking mold or foreign matter, and the gas permeable ring 30 can be integrally formed into a loop shape for the wool material (not shown). As shown in FIG. 2, or as shown in the preferred embodiment of FIG. 2, the air permeable ring 30 is made of a long material of a wool material, and the elongated body is bent to form a loop shape. The ring 30 is installed in the gap between the top surface of the top edge 11 of the casing 10 and the top surface of the sleeve groove 22 of the cover 20, so that the air of the outside can be combined with the cover 20 The peripheral gap between the sleeve groove 22 and the top edge 11 of the ring passes through the gas permeable ring 30 and the inner gap between the sleeve groove 22 and the top edge 11 to re-enter the combined casing 10 and the inside of the lid 20; When the venting ring 30 is assembled, the venting ring 30 can be placed in the grooving groove 22 by using a jig, so that the venting ring 30 can be assembled on the casing 10 along with the lid 20.

As shown in FIG. 2 and FIG. 3, the case 10 and the cover 20 are When the transparent or translucent material is made, the bottom surface of the casing 10 can be a flat surface, and the top of the lid 20 forms an arcuate curved surface 25 which is higher in the middle and lower in the periphery, so that the sterile culture box can be separated without being stacked. The curved surface of the cover 20 allows the external ambient light to be uniformly projected to the corners of the casing 10 through the curved surface of the top of the cover 20, so that the plants and the like in the sterile culture box receive uniform light.

As shown in FIG. 2 and FIG. 3, the case 10 and the cover 20 are When made of transparent or translucent material, the bottom surface of the casing 10 can be flat and A plurality of positioning portions 13 are disposed on the bottom surface of the casing 10, and a top portion of the cover 20 is formed with a lower curved portion 25 in the middle and a lower portion. The top surface of the cover 20 is provided with a plurality of upper positioning portions 24, and the upper positioning portions 24 are respectively Corresponding to the lower positioning portion 13, by stacking the plurality of sterile culture boxes 1 up and down, the lower positioning portion 13 of the bottom surface of the casing 10 of the upper sterile culture box and the upper positioning portion 24 of the top surface of the lid 20 of the lower sterile culture box can be utilized. The upper and lower alignments are combined and fixed.

As shown in FIG. 2 and FIG. 3, the bottom surface of the casing 10 is defined below The positioning portion 13 includes two lower positioning pieces 131 vertically spaced apart from each other. The positioning portion 24 on the top surface of the cover 20 includes two upper positioning pieces 241 which are vertically and oppositely spaced, as shown in FIG. When the positioning portion 24 and the lower positioning portion 13 are aligned in position, the upper positioning piece 241 and the lower positioning piece 131 are positioned to abut against each other.

As shown in Figure 5, when multiple sterile culture boxes are stacked on top of each other, The bottom surface of the upper casing 10 can be used as a plane, and the lower casing 20 is an arc-shaped curved surface 25 with a middle height and a low circumference, so that the outer casing between the casing 10 of the upper sterile culture box and the lid 20 of the lower sterile culture box can be used. The distance is larger, and the distance between the inner sections is smaller, so that ambient light can be projected through the curved top of the cover 20.

As shown in FIG. 2, FIG. 3 and FIG. 4, the casing 10 can be A plurality of spaced-apart indicator lines 14 are disposed on the transparent or translucent peripheral wall, and the marking function is provided by the indicator line 14 to facilitate the user to arrange the objects to be cultured in the casing 10.

When the creation is used, as shown in Figure 4, the system will train The substrate and the seedlings are waiting for the culture to be placed inside the casing 10. Referring to FIG. 3, the lid 20 provided with the venting ring 30 is attached to the casing 10, and then After being placed in a vacuuming apparatus for sterilization treatment, it can be taken out, such as a plant seedling waiting for culture or transportation of the culture, wherein the gas permeable ring 30 provided by the gas permeable gap between the lid 20 and the casing 10, Therefore, when the sterile culture box is applied to a plant or the like, the gas exchange function inside and outside the cartridge is provided, and the filtration function of blocking external mold or foreign matter is also provided.

As shown in Figure 2 and Figure 3, when the sterile culture box 1 is unique When placed vertically, the sterile culture box 1 can be projected by the box 10 and the lid 20 to the inside of the sterile culture box by the box 10 and the lid 20 of the transparent or translucent material, wherein By creating a curved surface with a middle height and a low circumference at the top of the cover 20, when the external ambient light passes through the curved surface of the top of the cover 20, it can be uniformly projected to all corners of the casing 10, and the plant can be cultivated in the aseptic culture box. The creature is evenly exposed to light.

As shown in Figure 5, when a plurality of sterile culture boxes 1 are stacked When placing or transporting, between the upper and lower sterile culture boxes 1, the lower positioning portion 13 of the bottom surface of the casing 10 of the upper sterile culture box 1 and the upper positioning portion 24 of the top surface of the lid 20 of the lower sterile culture box 1 are up and down. The position is fixed by combining, wherein the bottom surface of the box body 10 of the upper sterile culture box is a flat surface, and the lid 20 of the lower layer sterile culture box is an arc surface with a middle height and a low circumference, so that the upper box body 10 and the lower layer box cover 20 are The distance between the peripheral segments is larger, and the distance between the inner segments is smaller, so that ambient ambient light energy is projected through the top of the cover 20 to the inside of the casing 10, providing the light required for the growth of the culture, and by The curved surface of the top of the lid 20 is high in the middle and low in the periphery, so that the ambient light is evenly projected inside the sterile culture box, so that the plants and the like in the aseptic culture box are uniformly exposed to light.

1‧‧‧ sterile incubator

10‧‧‧Box

12‧‧‧Snap convex

121‧‧‧Extension

122‧‧‧Deduction Department

13‧‧‧ Positioning Department

131‧‧‧Lower positioning piece

20‧‧‧ lid

22‧‧‧ Sockets

23‧‧‧ buckle

231‧‧‧Bend

232‧‧‧Hook

24‧‧‧Upper Positioning Department

241‧‧‧Upper positioning piece

25‧‧‧Arc surface

30‧‧‧ breathable ring

Claims (13)

A sterile culture box comprising: a box body comprising a box mouth; a box cover, a detachable set covering the box mouth of the box body, forming a loop shape between the box cover and the box body and passing through The mouth of the box communicates with the air permeable gap inside and outside the sterile culture box; and a venting ring is filled in the air permeable gap between the box body and the lid. The aseptic culture box according to claim 1, wherein a plurality of snap protrusions are formed on an upper portion of the peripheral wall of the box body, and a plurality of buckle hooks are formed on a periphery of the bottom of the cover, and each buckle hook can be respectively buckled The snap protrusion of the case. The aseptic culture cassette according to claim 2, wherein the peripheral wall of the box body has a ring top edge at a periphery of the box mouth, and the snap convex portion cloth is formed under the ring top edge of the peripheral wall of the box body; An annular sleeve portion is formed at the bottom of the cover, and a sleeve groove is formed in the annular sleeve portion. The sleeve groove provides a top edge of the ring into which the outer peripheral groove side wall and the top edge of the ring groove are inside and outside. There is a gap between the side surfaces, and an assembly gap between the top surface of the top edge of the ring and the top surface of the groove of the sleeve groove is formed to communicate the gap between the inner and outer sides to form the air gap. The aseptic culture cartridge according to claim 3, wherein the snap projection comprises an extension extending horizontally from an outer peripheral surface of the side wall of the casing, and a fastening portion formed at an end of the extension; the middle portion of the buckle is formed a bent portion, the hook end has a hook portion, the cover is closed on the box body, and the annular sleeve portion at the bottom of the cover abuts against the extension of the buckle protrusion, and each hook can be bent The portion is bent downward and snapped to the fastening portion of the buckle protrusion. A sterile culture kit according to any one of claims 1 to 4, wherein The box body and the lid are formed into corresponding polygons, and the box body and the lid respectively form outer arc angles at respective corners thereof. The aseptic culture cassette according to any one of claims 1 to 4, wherein the box body and the lid are transparent or translucent plastic parts, and the bottom surface of the box body is flat, and a plurality of lower positioning portions are arranged on the bottom surface of the box body. The top of the lid is formed with a curved upper surface and a lower curved surface. The top surface of the lid is distributed with a plurality of upper positioning portions, and the upper positioning portions respectively correspond to the lower positioning portions, and can be utilized when the aseptic culture boxes are stacked on top of each other. The lower positioning portion of the bottom surface of the upper casing is fixed to the upper positioning portion of the top surface of the lower cover. The aseptic culture box according to claim 5, wherein the box body and the lid are transparent or translucent plastic parts, the bottom surface of the box body is flat, and a plurality of lower positioning portions are arranged on the bottom surface of the box body, and the top of the box cover is formed in the middle. The upper and lower curved surfaces of the cover are arranged on the top surface of the cover, and the upper positioning portions respectively correspond to the lower positioning portions for respectively utilizing the bottom surface of the upper casing when the aseptic culture boxes are stacked one on top of the other The lower positioning portion is fixed to the upper positioning portion of the top surface of the lower cover. The aseptic culture cassette according to claim 7, wherein the positioning portion below the bottom surface of the box body comprises two lower positioning pieces which are vertically and oppositely spaced, and the positioning portion on the top surface of the box cover is vertically spaced apart from each other. The upper positioning piece is arranged, and when the upper positioning part is combined with the lower positioning part, the upper positioning piece and the lower positioning piece are positioned against each other. The aseptic culture cartridge according to any one of claims 1 to 4, wherein the cartridge body and the lid are transparent or translucent plastic members, and the lower portion of the peripheral wall of the cartridge body is provided with a plurality of spaced-apart marking lines. The sterile culture kit of claim 8, wherein the cartridge body is The lower part of the wall is provided with a plurality of lines arranged at intervals. The sterile culture cartridge according to any one of claims 1 to 4, wherein the gas permeable loop is a cotton annular ring. The sterile culture cartridge of claim 8, wherein the gas permeable loop is a cotton annular ring. The sterile culture cartridge of claim 10, wherein the gas permeable loop is a cotton annular ring.