JPH0352687Y2 - - Google Patents

Description

[Detailed description of the invention] (Field of industrial application) The present invention is intended for use in aerobic culture vessels such as plant culture vessels, especially tissue culture vessels, or culture and growth vessels for aerobic bacteria such as enzymes, bacteria, molds, and mushrooms. Regarding the stopper.

(Conventional technology) Conventionally, when culturing plants in a culture container,
In order to prevent bacteria from entering the culture container from the atmosphere and to supply the air necessary for culture into the culture container, the opening of the culture container is equipped with a vent plug that allows air to circulate. are being blocked.

Conventionally, the plugs used to close the openings of the culture vessels have generally been cotton plugs, rubber plugs, aluminum foil plugs,
Aluminum stoppers, plastic stoppers, etc. are used.

However, cotton plugs allow air to pass through as well as bacteria, making them unreliable in preventing bacteria from entering. Furthermore, since cotton plugs hardly transmit light, when used as plugs for plant culture containers, they have the disadvantage that they cannot take in the light that is essential for plant growth.

Rubber stoppers have the disadvantage that they do not transmit any light at all, and since rubber stoppers are porous, it is easy for bacteria to accumulate inside them, and it is difficult to remove these bacteria from rubber stoppers. There is also the possibility of back contamination to other culture vessels.

Aluminum foil stoppers, aluminum stoppers, and plastic stoppers are inherently non-porous, so they must be plugged with a slight gap formed at the opening of the culture container, and therefore, there is no need to allow air to leak through that gap. There is a risk of bacteria invading.

In response to these, recently it has been proposed to use a microporous sheet, a so-called main brain filter, as a stopper for a culture container in order to prevent the invasion of various bacteria.

(Problem to be solved by the invention) Although the above microporous sheet allows ventilation to pass through the pores of the sheet, it does not allow bacteria to pass through, so it is possible to prevent bacteria from entering the container. I can do it.

However, when microporous sheets are used as ventilation plugs in culture containers, they improve air permeability and at the same time increase moisture permeability, which causes the problem that the culture medium inside the culture containers dries out, and conversely This resulted in a decrease in yield. Moreover, this microporous sheet has a low light transmittance and has the disadvantage that it cannot take in the light that is essential for plant growth.

This invention solves the above-mentioned conventional drawbacks, and its purpose is to easily ventilate the required amount of air, prevent the invasion of bacteria and mites from the outside, and make it easy to clean. The object of the present invention is to provide a vent stopper that is not only easy to sterilize but also has good light transmittance and does not dry out the inside of the container.

(Means for Solving the Problems) The vent plug of the present invention is a vent plug that is attached to an opening of a container to close the opening in a breathable state, and has a transparent translucent The present invention has a hydrophobic air-permeable sheet attached to the transparent film to close the openings of the transparent film, thereby achieving the above object.

(Example) An example of this example will be described below based on the drawings.

Example 1 As shown in FIGS. 1 and 2, the vent plug 2 of the present invention includes a square transparent film 1 having a circular opening 10 approximately in the center, and the transparent film 1. A hydrophobic breathable sheet 11 is attached to a transparent film 1 to close an opening 10 of the film.

The air-permeable sheet 11 is attached to the edge of the opening 10 of the translucent film 1 for the entire week by a commonly used adhesive method, heat fusion method, or the like.

The light transmittance of the translucent film 1 used in the ventilation plug 2 of this example is not particularly limited, and can be set depending on the application. For example, when used as the ventilation plug 2 of a plant culture container, the light transmittance of the film 1 is preferably 20% or more from the viewpoint of plant growth. Specific examples of the translucent film 1 include plastic films made of polyamide, polyester, polyoffine, and the like.

The breathable sheet 11 is made of a hydrophobic resin such as polyethylene, polypropylene, polyvinyl chloride, or fluorine resin, and the pore size can be adjusted by stretching, foaming, etc.
Innumerable micropores of 0.01 to 100 μm are formed. This breathable sheet 11 has good air permeability, prevents impurities such as germs and dust from entering, and also has the function of blocking the passage of moisture to prevent the inside of the container from drying out. The breathable sheet 11 has a porosity of 15 to 90% and a thickness of 20 to 90%.
A thickness of 500 μm is particularly suitable for practical use.

The sheet-like ventilation plug 2 having the above structure is used in the following manner.

As shown in FIG. 3, the ventilation plug 2 is placed in the upper end opening 3a of the plant tissue culture container 3 so that the ventilation sheet 11 is located in the center of the opening 3a, and The peripheral end 2a of the vent plug 2 is folded and placed along the outer peripheral surface of the culture container 3, and a rubber band 4 or the like is wrapped around the outside of the vent plug 2 and tightened.

According to the above configuration, light can pass through the transparent film 1 and enter the culture container 3. Therefore, when culturing plant tissue, a large number of test tubes or flask-shaped culture containers 3 are arranged side by side and the culture is performed by shining light from above, and the light can be taken into the culture containers 3 without any problem. It can promote photosynthesis in plants. Further, since the air-permeable sheet 11 has air permeability, the air inside the culture container 3 can be ventilated, and the ethylene gas generated inside the culture container 3 can be easily discharged. Note that the part where light is blocked by this breathable sheet 11 is very small, and it is possible to take in light from above with the other part of the translucent film. Efficiency is not hampered. Moreover,
According to the above configuration, the aeration plug 2 can be easily attached to the culture container 3, and the aeration plug 2 can be easily attached at the cultivation site without requiring any special equipment.

Note that the thickness, shape, dimensions, etc. of the breathable sheet 11 and the transparent film 1 are not limited at all, and can be changed as appropriate depending on the purpose of use, use, etc. For example, as shown in FIG. 4, the openings 10 provided in the translucent film 1 and the breathable sheet 11 may have a rectangular shape.
The air permeability of the ventilation plug 2 can be freely adjusted by the pore diameter of the air permeable sheet 11 and the effective area (area of the opening 10 of the translucent film 1), and can be adjusted depending on the type of plant to be cultured and the container. It can be selected depending on the size of the cell and the culture environment.

Furthermore, an opening 1 formed in the translucent film 1
0 may be provided in plural numbers, and the transparent film 1
It may also be formed by providing a slit-like incision from the end. The vent plug 2 of the present invention is not limited to the vent plug 2 of the culture container 3 for culturing plant tissue, but can also be used as a vent plug of other containers.

Embodiment 2 FIG. 5 shows a vent plug 2 in which a reinforcing sheet 12 is adhered to the entire lower surface of the breathable sheet 11 by heat fusion. This reinforcement sheet 1
2 over the entire surface of the breathable sheet, the breathable sheet 11 is reinforced, and the breathable sheet 11 can be prevented from being easily damaged by external forces.

The reinforcing sheet 12 is made of a material that has a higher air permeability than the air permeable sheet 11 and has thermal adhesive properties. Specific examples of the reinforcing sheet 12 include (a) a nonwoven fabric made of polyamide, polyester, polyolefin, etc.; The diameter or air permeability of the pores of the adhesive plastic sheet is set larger than that of the air permeable sheet 11), or a sheet in which countless air holes are formed in a c-plastic sheet by punching or the like.

The aeration plug 2 constructed in this manner may be attached to the culture container 3 with a rubber band 4 as in the first embodiment, or as shown in FIG. It may be attached to the flange 3b formed at the upper end by adhesion or fusion. When attaching the periphery of the aeration plug to the flange 3b of the culture container 3 by adhesion or fusion, the transparent film 1 of the aeration plug 2 can be attached to the culture container 3 in an airtight manner. This is advantageous when high precision in preventing the intrusion of germs is required. Further, the reinforcing sheet 12 can also be attached to both sides of the breathable sheet 11.

Example 3 The ventilation plug 2 shown in Example 1 is prepared in a state where a large number of pieces are manufactured in a continuous band shape and wound into a roll, as shown in FIG. 7, for example.

As shown in FIG. 8, this roll-shaped ventilation plug 21 is made by forming openings 10 at predetermined intervals in a band-shaped transparent film 1, and pasting a breathable sheet 11 into the openings 10. The belt-shaped ventilation plug 20 is manufactured by winding the belt-shaped ventilation plug 20 around the cylindrical core body 5 in a roll shape, as shown in FIG. A perforation 13 is formed in the translucent film 1 at an intermediate position between adjacent openings 10, 10.
is provided across the width direction of the translucent film 1,
By cutting the translucent film 1 at the perforation 13, each ventilation plug 2 is separated from the rolled ventilation plug 21.

According to the above configuration, when using the ventilation plug 2, the ventilation plug 2 is divided into pieces at the perforations 13 and separated into individual pieces.
It is easy to handle because it can be removed. Moreover, since the rectangular vent plug 2 can be continuously removed from the rolled vent plug 21 when the vent plug 2 is placed over the culture container 3, automation is also possible.

(Effect of the invention) As described above, since the aeration plug of the present invention allows light to pass through the translucent film portion, the culture efficiency does not decrease when culturing plants in a container. Moreover, the breathable sheet part prevents bacteria and mites from entering from the outside.
Can be ventilated. Furthermore, since it is difficult for germs to accumulate within the breathable sheet, cleaning and sterilization of the vent plug is easy. Furthermore, the breathable sheet prevents the passage of moisture and does not increase moisture permeability, so the medium in the culture container does not dry out.
There is no reduction in culture yield.

[Brief explanation of the drawing]

FIG. 1 is a plan view of a ventilation plug according to an embodiment of the present invention;
Fig. 2 is a sectional view taken along the - line in Fig. 1, Fig. 3 is a longitudinal sectional view of the main part showing the installation state of the vent plug, Fig. 4 is a plan view of the vent plug of another embodiment, and Fig. 5 is FIG. 6 is a cross-sectional view of a ventilation plug according to still another embodiment, FIG. 6 is a vertical sectional view of a main part showing how the ventilation plug is installed, and FIG. The figure is a plan view of the main part of the rolled vent plug in an extended state. 1... Transparent film, 3... Container, 3a...
Opening, 10... Opening, 11... Breathable sheet, 12... Reinforcement sheet.

Claims (1)

[Claims for Utility Model Registration] 1. A vent plug attached to an opening of a container to close the opening in a breathable state, comprising a translucent film having an opening, and the translucent film. A vent plug having a hydrophobic breathable sheet attached to a translucent film to close an opening in the film. 2. The vent plug according to claim 1, wherein the transparent film is formed in a rectangular shape, and the opening is formed approximately in the center of the transparent film. 3. The translucent film is formed into a belt shape, and a large number of openings are formed in the translucent film at predetermined intervals along the longitudinal direction of the translucent film. Scope of claim for utility model registration No. 1 wound into a roll
Vent plugs as described in section. 4. Any one of claims 1 to 3 of the utility model registration claims, wherein the breathable sheet is heat-sealed over the entire surface with a reinforcing sheet having a higher air permeability than the air permeability of the breathable sheet. Vent plug as described in .