TWM447512U - DNA sample imaging and recognition system for electrophoresis - Google Patents

Description

DNA sample imaging recognition system for electrophoresis

This creation is about a DNA sample imaging system for electrophoresis, especially a system that can simultaneously perform electrophoresis and DNA sample identification.

At present, the electrophoresis experiment program of the formula, the electrophoresis tank must be connected with other power supply equipment to provide the power required for the electrophoresis process, so it is often necessary to interface a lot of wires in the connection of equipment and equipment. Furthermore, during the experiment, the user has to wait until the electrophoresis process of the sample is completely completed, and then the block gel containing the sample can be placed under the ultraviolet imaging device, and the result is examined by ultraviolet irradiation. If ultraviolet light is accidentally applied to the skin of the user, there is a risk of carcinogenicity in the long run. In addition, since the electrophoresis experiment process is often extremely time consuming, if it is a block If the electrophoresis result displayed on the gel is incorrect, the user must re-execute the electrophoresis process with the sample; therefore, it is very inconvenient and time consuming in the entire experimental procedure.

The present invention mainly integrates the illumination base and the camera device into the conventional electrophoresis device, so as to provide the user with instant identification and observation and recording of the DNA sample during the electrophoresis, and can be photographed or The way of photography to completely record the electrophoresis process of DNA samples, or to stop and restart the electrophoresis process immediately when an error occurs during electrophoresis, without the need for a traditional electrophoresis process, it is necessary to wait for a lengthy electrophoresis process before further The special light source is irradiated to check or confirm whether the state of the DNA sample after the electrophoresis process is correct, thereby greatly saving the time required for the DNA sample to perform the electrophoresis process.

A DNA sample imaging recognition system for electrophoresis, comprising: a lighting base, a mask shell, a base and a camera device; the mask shell is disposed above the lighting base; the base is It is fixed on the top surface of the mask casing; the camera device is mounted on the base. The pedestal includes: a pedestal, a channel, a extraction frame, a filter and a support frame; the channel is disposed inside the pedestal; the extraction frame is installed inside the pedestal The filter is disposed on the extraction frame; the support frame is mounted on a side of the base, and one side of the support frame is fixed to the imaging device. Among them, the camera device uses a digital camera. Lighting base, including: seat and electricity The source supply device; and the two sides of the base body are respectively extended with a platform; wherein the power supply device is disposed inside the base body. The power supply device can also be installed inside the platform. The inside of the two platforms is provided with a groove; and the inside of the groove is provided with a lamp body structure; the opening of the groove is provided with a transparent partition; and the side of the transparent partition is further set There is a filter; wherein the lamp body structure is a blue LED lamp set. The top surface of the platform on one side of the base body is provided with a control panel; and the top surface of the platform on the other side of the base body is provided with two connection slots; wherein the control panel and the two connection slots are connected to the power supply The supply devices are electrically connected to each other.

The review committee can have a better understanding of the technical features of the present invention. The following is a detailed description of the specific embodiments and the drawings.

For the first embodiment, please refer to the first to tenth drawings; a DNA sample imaging recognition system for electrophoresis, comprising: a lighting base D1, a mask housing A1, a base and an imaging device; the mask housing A1 The pedestal is mounted on the top surface of the illuminating base D1; the pedestal is mounted on the pedestal. The pedestal includes: a pedestal B1, a channel B2, an extraction frame B3, a filter B31 and a support frame B4; the channel B2 is disposed inside the pedestal B1; the extraction frame B3 is The channel B2 is disposed in the interior of the platform B1; the filter B31 is disposed on the extraction frame B3; the support frame B4 is mounted on the side of the platform B1, and the support frame B4 One side is fixed to the imaging device. Among them, the image pickup apparatus uses a digital camera C11.

The lighting base D1 includes: a seat body D2 and a power supply device; and two sides of the seat body D2 are respectively extended with a platform D3; wherein the power supply device is disposed inside the seat body D2. The power supply device can also be installed inside the platform D3. The inside of the two platforms D3 is provided with a groove D33; and the inside of the groove D33 is provided with a lamp body structure D331; the opening of the groove D33 is provided with a transparent partition D332; and the transparent partition The side of the board D332 is further provided with a filter D333; wherein the lamp body structure D331 is a blue LED lamp set. The top surface of the platform D3 on the side of the base D2 is provided with a control panel D31; and the top surface of the platform D3 on the other side of the base D2 is provided with two connection slots D32; wherein the control panel D31 and the second The connection slot D32 is electrically connected to the power supply device.

The illumination base D1 is mainly disposed by the power supply device, so that when the electrophoresis tank 5 is placed across the illumination base D1, the connection wire 6 can be used to connect to the electrode end of the electrophoresis tank 5, thereby directly from the illumination base. D1 provides power for the electrophoresis tank 5, so that it does not need to additionally interface with other power supply equipment. Furthermore, the blue light source provided by the blue LED lamp set installed in the groove D33 on the inner side of the platform D3 on both sides of the illumination base D1 can activate the fluorescent dye on the agar colloid 7 for use. While performing the electrophoresis experiment, the state display of the DNA sample 71 during the electrophoresis can be observed. The filter D333 on the side of the transparent spacer D332 is used to filter out the blue light source. He does not need the source band, but only the blue band that effectively excites the fluorescent dye.

The mask housing A1 is disposed above the illumination base D1 on which the electrophoresis tank 5 is installed, so as to shield other interference light sources existing in the external environment during the electrophoresis process; The inside of the cover case A1 is relatively provided with a darkroom space, so that the state of the fluorescent dye on the agar colloid 7 can be more clearly displayed during the electrophoresis of the DNA sample 71. The filter B31 of the pedestal is used to filter the blue light source band used to excite the fluorescent dye of the agar colloid 7 during electrophoresis, so that the user can not be dissipated in the space during observation. The light damage effect of the blue light source can further clearly check the state and change of the fluorescent dye on the agar colloid 7. The lens of the digital camera C11 is disposed in the channel B2 of the base and opposite to the filter B31 of the base; and the support frame B4 of the base is used to The digital camera C11 is locked to the base; wherein the passage B2 of the base is connected to the inside of the mask housing A1.

The user can simultaneously view and observe the state and change of the fluorescent dye of the DNA sample 71 on the agar colloid 7 during the electrophoresis process, and can also take pictures or record. The electrophoresis process is recorded and retained so that relevant personnel can perform subsequent comparisons, analyses or data preservation. In addition, during the electrophoresis process, the user can find any error state by synchronous observation and inspection, and the user can immediately interrupt the electrophoresis process and restart the electrophoresis process, thereby greatly reducing the waste of experimental failure or error. Time between.

For the second embodiment, please refer to the eleventh to fourteenth, fifth to eighth, fifteenth to sixteenth and tenth embodiments; a DNA sample imaging recognition system for electrophoresis, comprising: a lighting base D1, a mask housing A1, a base and an imaging device; the mask housing A1 is disposed above the lighting base D1; the base is fixed on the top surface of the mask housing A1 The camera device is mounted on the base. The pedestal includes: a pedestal B1, a channel B2, an extraction frame B3, a filter B31 and a support frame B4; the channel B2 is disposed inside the pedestal B1; the extraction frame B3 is The channel B2 is disposed in the interior of the platform B1; the filter B31 is disposed on the extraction frame B3; the support frame B4 is mounted on the side of the platform B1, and the support frame One side of B4 is fixed to the imaging device.

The camera device is provided with a transmission interface; the transmission interface can link and transmit the image signal captured by the camera device to the computer system 9, the handheld device or the network device; wherein the camera device is used CMOS lens module C12; the transmission interface uses the USB transmission line C2. The camera device can also use a CCD lens module; the transmission interface can also use a wireless transmitter. The lighting base D1 includes: a seat body D2 and a power supply device; and two sides of the seat body D2 are respectively extended with a platform D3; wherein the power supply device is disposed inside the seat body D2. The power supply device can also be installed inside the platform D3. The inner side of the two platforms D3 is provided with a groove D33; and the inside of the groove D33 is provided with a lamp body structure D331; The opening of the groove D33 is provided with a transparent partition D332; and the side of the transparent partition D332 is further provided with a filter D333; wherein the lamp body structure D331 uses a blue LED lamp set. The top surface of the platform D3 on the side of the base D2 is provided with a control panel D31; and the top surface of the platform D3 on the other side of the base D2 is provided with two connection slots D32; wherein the control panel D31 and the second The connection slot D32 is electrically connected to the power supply device.

The illumination base D1 is mainly disposed by the power supply device, so that when the electrophoresis tank 5 is placed across the illumination base D1, the connection wire 6 can be used to connect to the electrode end of the electrophoresis tank 5, thereby directly from the illumination base. D1 provides power for the electrophoresis tank 5, so that it does not need to additionally interface with other power supply equipment. Furthermore, the blue light source provided by the blue LED lamp set installed in the groove D33 on the inner side of the platform D3 on both sides of the illumination base D1 can activate the fluorescent dye on the agar colloid 7 for use. While performing the electrophoresis experiment, the state display of the DNA sample 71 during the electrophoresis can be observed. The filter D333 on the side of the transparent spacer D332 is used to filter out other unnecessary light source bands in the blue light source, and only retains the blue light band which can effectively excite the fluorescent dye.

The mask housing A1 is disposed above the illumination base D1 on which the electrophoresis tank 5 is installed, so as to shield other interference light sources existing in the external environment during the electrophoresis process; The inside of the cover case A1 is relatively provided with a darkroom space, so that the state of the fluorescent dye on the agar colloid 7 can be more clearly displayed during the electrophoresis of the DNA sample 71. The base The filter B31 is used to filter out the blue light source band used to excite the fluorescent dye of the agar colloid 7 during electrophoresis, so that the user does not receive the blue light source dissipated in the space during observation. The influence of light damage can further clearly check the state and change of the fluorescent dye on the agar colloid 7. The CMOS lens module C12 is disposed in the channel B2 of the pedestal and is disposed opposite to the filter B31 of the pedestal; and the support frame B4 of the pedestal is used to The CMOS lens module C12 is locked to the base; wherein the channel B2 of the base is connected to the inside of the mask housing A1.

The user can capture the image signal from the CMOS lens module C12 during the electrophoresis process, and connect and transmit the image signal to the computer system 9 through the USB transmission line C2, so that the user can simultaneously view and observe the DNA inspection on the computer system 9. The state and change of the fluorescent dye on the gelatin 7 of the body 71; the operation of the monitoring and analysis software in the computer system 9 can be used to record and retain the electrophoresis process by taking a photo or recording it into an electronic file. Or use the computer system 9 for data processing so that relevant personnel can perform subsequent comparisons, analysis or data preservation. In addition, during the electrophoresis process, the user can find any error state by synchronous observation and inspection, and the user can immediately interrupt the electrophoresis process and restart the electrophoresis process, thereby greatly reducing the waste of experimental failure or error. time.

The above embodiments are merely illustrative of the principles and functions of the present invention, and are not intended to limit the present invention; therefore, modifications and variations of the above-described embodiments will be apparent to those skilled in the art. This creation has the industrial applicability, novelty and progress, and meets the requirements of new patents.

1‧‧‧Sensor identification system

A1‧‧‧mask housing

B1‧‧‧ Terrace

B2‧‧‧ channel

B3‧‧‧ extraction framework

B31‧‧‧Filter

B4‧‧‧Support frame

C11‧‧‧ digital camera

C12‧‧‧CMOS lens module

C2‧‧‧USB transmission line

D1‧‧‧Lighting base

D2‧‧‧ body

D3‧‧‧ platform

D31‧‧‧Control panel

D32‧‧‧ connection slot

D33‧‧‧ Groove

D331‧‧‧Light body structure

D332‧‧‧Transparent partition

D333‧‧‧Filter

5‧‧‧electrophoresis tank

6‧‧‧Connecting wire

7‧‧‧Agarage colloid

71‧‧‧DNA samples

8‧‧‧ Buffer

9‧‧‧ computer system

The first picture is a perspective view of the creation; the second picture is an exploded view of the creation; the third picture is a combination of a digital camera, a base and a mask housing; and the fourth picture is a digital camera, a base and a component of the mask housing. Explosion diagram; the fifth diagram of the illumination base (1); the sixth diagram of the illumination base (2); the seventh diagram of the component base of the illumination base; the eighth diagram of the embodiment of the creation (1); Figure 9 (II); the state diagram of the gelatin of the acacia after electrophoresis; the perspective view of the creation of the eleventh figure; the exploded view of the component of the twelfth figure; CMOS lens module, pedestal and mask housing combination diagram; Figure 14 CMOS lens module, pedestal and mask housing component exploded view; fifteenth figure embodiment of the creation of the creation (1) Figure 16 is a diagram (2) of an embodiment of the present creation.

1‧‧‧Sensor identification system

A1‧‧‧mask housing

B4‧‧‧Support frame

C11‧‧‧ digital camera

D1‧‧‧Lighting base

Claims (10)

A DNA sample imaging recognition system for electrophoresis, comprising: a lighting base, a mask shell, a base and a camera device; the mask shell is disposed above the lighting base; the base is Is mounted on the top surface of the mask housing; the camera device is mounted on the base; wherein the base includes: a seat and a filter; and the filter is Set inside the platform. The DNA sample imaging recognition system for electrophoresis according to the first aspect of the invention, wherein the pedestal further comprises: a channel and an extraction frame; the channel is disposed inside the platform; The extraction frame is the channel installed inside the platform; and the filter is disposed on the extraction frame. The DNA sample imaging recognition system for electrophoresis according to the second aspect of the invention, wherein the camera device is provided with a transmission interface; the transmission interface can be connected to the image captured by the camera device. Transfer to a computer system, handheld device or network device. The DNA sample imaging system for electrophoresis according to any one of claims 1 to 3, wherein the illumination base comprises: a seat body; wherein, on both sides of the seat body, respectively The platform is extended to have a groove; the inner side of the platform is provided with a groove; and the inside of the groove is provided with a lamp body structure; and the opening of the groove is provided with a transparent partition. The DNA sample imaging system for electrophoresis according to the fourth aspect of the invention, wherein the lighting base further comprises: a power supply device; the power supply device is a device or the platform Internal. The DNA sample imaging recognition system for electrophoresis according to claim 5, wherein a top surface or a side surface of the platform on one side of the body is provided with a control panel; and the other side platform of the base body is provided. The top surface or the side surface is provided with a plurality of connecting slots; wherein the control panel and the connecting slots are electrically connected to the power supply device. The DNA sample imaging system for electrophoresis according to the sixth aspect of the invention, wherein the side of the transparent partition is further provided with a filter. The DNA sample imaging system for electrophoresis described in claim 7, wherein the lamp body structure, in particular, a blue LED lamp group. The DNA sample imaging system for electrophoresis according to the eighth aspect of the invention, wherein the base further comprises: a support frame; the support frame is mounted on a side of the platform; One side of the support frame is fixed to the camera device; the camera device can use a camera or a lens module; the transmission interface is especially a signal transmission line or a wireless transmitter. The DNA sample imaging recognition system for electrophoresis according to claim 9, wherein the channel of the pedestal is connected to the inside of the mask case; the camera device, in particular, an index A camera, CMOS lens module or CCD lens module.