五、新型說明： 【新型所屬之技術領域】 [0001] 本新型有關一種光產生裝置，尤指一種螢光產生裝 置。 [先前技術3 [0002] 進行遺傳學、分子生物學等基因研究或動植物疫病 的檢測時，需先以擴增.核酸的手段（例如聚合酶連鎖反應 ，Polymerase Chain Reaction, PCR)，將少量的核 酸樣本在短時間内複製擴增到可以被偵測到的量。上述 核酸擴增產物可進一步經由雜合作用（Hybridization) 而使目標核酸片段與帶有螢光、放射物質或呈色酵素的 核酸揲針（probe)連結，產生螢光、放射影像或呈色反應 。目前在許多的生物晶片上採用螢光染劑做為標識物， 主要因為螢光染劑能夠提供較好的分析結果，若與傳統 的呈色劑方法相較，螢光染劑可提供高約1 000倍至50萬 倍的靈敏度。 [0003] 螢光顯微鏡技術（Fluorescence Microscopy)、 螢光分析儀、流式細胞儀、相機（Camera)或影像擷取裝 置等裝置用以擷取螢光影像後進行觀察、偵測及分析上 述的螢光反應。這些技術都涉及到激發光源的選用，因 為特定的螢光染劑需要使用特定波長範圍的光來當作激 發光源，當一個適當波長的光照射具有螢光性質的分子 時，分子會吸收光的能量而被激發至高能量狀態，並在 極短時間（10_8〜10_4秒）内回復至低能量狀態，同時以 放光的形式將多餘能量釋出，因此必需依照螢光染劑的 表單編號A0101 第3頁/共13頁 [0004]M430003 特性選擇與之匹配的激發光源，始能得到最佳染劑激發 效果（Exci tation)。 激發光源的選擇包含有以一般光源發出紫外光或雷 射光等，但紫外光容易散射，且傳輸及穿透不易，因此 ，檢測儀器必須使用特殊光學元件以具有高度紫外光靈 敏度，使得其造價成本高，不符合經濟效益。而若使用 雷射光作為激發光源，其具有波長單一、穿透性高而容 易進行偵測，但雷射激發光必須配合濾鏡及分光鏡的使 用，其體積佔據大，而使得其儀器的架設困難。 [0005] 因此，如中華民國專利公開201 01 8728號的「用以 激發螢光訊號的激發光源j ，其揭露一種利用可調整發 光強度以及光色組合的發光二極體模組作為激發光源， 透過光色組合調整的方式取得可激發螢光染劑的激發波 長。但螢光染劑僅在特定波長的激發光源下才可獲得較 佳的激發效率，且發光二極體模組的光源的波長範圍容 易與螢光染劑激發後的螢光波長範圍有部分的重疊，而 造成量測時無法區別光源訊號的強度為激發後的螢光或 發光二極體模組的光源，造成準確性不佳的問題。 【新型内容】 [0006] 本新型的主要目的，在於提供一特定波長範圍的激 發光源，以有效提升激發螢光的產生效率。 本新型的另一目的，在於解決習知的激發光源的波 長範圍容易與螢光光源的波長範圍重疊，而造成螢光激 發量偵測困難的問題^ 表單編號A0101 第4頁/共13頁 [0007] [0008] [0009] [0010] [0011] [0012] [0013] 為達上述目的，本新型提供一種螢光產生裝置，包 含有一發出一光束的藍光二極體、一濾光件以及一螢光 染劑。該濾光件設置於該藍光二極體的一光射出方向以 接收該光束並轉換輸出為一過濾光束，該過濾光束的波 長介於465nra至505nm之間，該螢光染劑設置於該渡光件 相對該藍光二極體的一側以接收該過濾光束並激發出一 螢光。 由上述說明可知，相較於習知技術，本新型具有下 列特點： 一、 藉由該濾光件控制該過濾光束的波長範圍於 465nm至505nm之間，而以高效率且精準控制波長的方式 激發該螢光染劑獲得該螢光。 二、 藉由設定該過滤光束的波長範圍，可避免該過/ 濾光束的波長範圍與螢光波長範圍重疊或部分重疊，造 成量測上的誤差問題。 【實施方式】 有關本新型的詳細說明及技術内容，現就配合圖式 說明如下： 請參閱「圖1」及「圖2」所示，本新型為一種螢光 產生裝置，包含有一發出一光束11的藍光二極體10、一 濾光件20以及一螢光染劑30。該濾光件20設置於該藍光 二極體10的一光射出方向以接收該光束11並轉換輸出為 一過濾光束21，該過遽光束21的波長介於465nm至505nm 之間，該螢光染劑30設置於一試管32内，並使該試管32 表單编號A0101 第5頁/共13頁 M430003 置放於該濾光件20相對該藍光二極體1〇的一側以接收該 過濾光束21並激發出一螢光31，而藉由一檢測模組4〇進 行光頻譜的檢測’於本實施例中，該藍光二極體1〇、該 濾光件20以及該桧測模組40與一電腦50連接，該電腦5〇 控制該藍光二極體10的發光強度以及開關、該濾光件2〇 的濾波範圍以及接收該檢測模組40的資訊。除此之外， 本新型更設置有一加熱件60以利PCR反應的進行。 [0014] [0015] 一般來說，請配合參閱「圖3」所示，光波長於頻譜 中的顯示是於一範圍内具有一峰值以及由該峰值往兩側 逐漸衰減，而波長的數值僅指該峰值所對應的數值，並 非代表光在單一波長具有量值而呈現脈衝形式。舉例來 說’本新型所稱的波長為488nm，指其光波的峰值於 488nm處，且488nm的前後波段中仍具有量值。因此，若 藍光一極體10的光源波長不夠純一，容易造成藍光二極 體1 〇發出的光束11的部分波長範圍與激發後的螢光31波 長重疊’使得該檢測模組40於檢測時一併接收到該光束 Π的光能量，造成檢測誤差的問題。 本新型透過該濾光件20對該藍光二極體10進行濾光 而得到該過濾光束21而對應該螢光染劑30的激發波長， 該螢光染劑30可為6-螢光素氨基磷酸酯（6-FAM)、5-螢光素氨基磷酸酯（5-FAM)、俄勒岡綠-488 (OregonV. New description: [New technical field] [0001] The present invention relates to a light generating device, and more particularly to a fluorescent generating device. [Prior Art 3 [0002] When performing genetic research such as genetics, molecular biology, or animal and plant disease, a small amount of the first method is to amplify the nucleic acid (for example, polymerase chain reaction, PCR). The nucleic acid sample is replicated in a short period of time to an amount that can be detected. The nucleic acid amplification product may further bind a target nucleic acid fragment to a nucleic acid probe with a fluorescent, radioactive or coloring enzyme via hybridization to generate a fluorescent, radiographic or color reaction. . Fluorescent dyes are currently used as markers on many biochips, mainly because fluorescent dyes provide better analytical results. Fluorescent dyes provide high yields compared to conventional color formers. 1 000 to 500,000 times sensitivity. [0003] Fluorescence Microscopy, Fluorescence Analyzer, Flow Cytometry, Camera, or Image Capture Device are used to capture, detect, and analyze the above-mentioned fluorescent images. Fluorescent reaction. These techniques involve the selection of excitation sources, because specific fluorescent dyes require the use of light of a specific wavelength range as the excitation source. When a light of the appropriate wavelength illuminates a molecule with fluorescent properties, the molecule absorbs light. The energy is excited to a high energy state, and returns to a low energy state in a very short time (10_8~10_4 seconds), and at the same time, the excess energy is released in the form of light emission, so it is necessary to follow the form number A0101 of the fluorescent dye. 3 pages / total 13 pages [0004] M430003 Features Select the matching excitation source to get the best dye excitation (Excitation). The selection of the excitation light source includes ultraviolet light or laser light emitted by a general light source, but the ultraviolet light is easily scattered, and transmission and penetration are not easy. Therefore, the detection instrument must use a special optical element to have high ultraviolet light sensitivity, so that the cost thereof is costly. High, not in line with economic benefits. However, if laser light is used as the excitation light source, it has a single wavelength and high penetration and is easy to detect. However, the laser excitation light must be used together with the filter and the beam splitter, and its volume is large, which makes the instrument erect. difficult. [0005] Therefore, the excitation light source j for exciting a fluorescent signal is disclosed in the Republic of China Patent Publication No. 201 01 8728, which discloses a light-emitting diode module using an adjustable luminous intensity and a combination of light colors as an excitation light source. The excitation wavelength of the fluorescent dye can be excited by the combination of light and color. However, the fluorescent dye can obtain better excitation efficiency only under the excitation light source of a specific wavelength, and the light source of the LED module is The wavelength range is easily overlapped with the fluorescence wavelength range after the fluorescent dye is excited, and the intensity of the light source signal cannot be distinguished when measuring, and the light source of the fluorescent or light-emitting diode module after excitation is generated, resulting in accuracy. The problem is not good. [New content] [0006] The main purpose of the present invention is to provide an excitation light source of a specific wavelength range to effectively improve the efficiency of generating fluorescent light. Another object of the present invention is to solve the conventional problem. The wavelength range of the excitation light source easily overlaps with the wavelength range of the fluorescent light source, which causes difficulty in detecting the fluorescence excitation amount. ^Form No. A0101 Page 4 of 13 [0010] [0012] [0012] In order to achieve the above object, the present invention provides a fluorescence generating device comprising a blue LED emitting a light beam and a filter And a fluorescent dye disposed on a light emitting direction of the blue LED to receive the light beam and converted into a filtered light beam having a wavelength between 465 nra and 505 nm. The light dyeing agent is disposed on a side of the light-emitting member opposite to the blue-light diode to receive the filtered light beam and to excite a fluorescent light. As can be seen from the above description, the present invention has the following features compared with the prior art: The filter beam is controlled by the filter to have a wavelength ranging from 465 nm to 505 nm, and the fluorescent dye is excited to obtain the fluorescent light with high efficiency and precise wavelength control. 2. By setting the filtered beam In the wavelength range, the wavelength range of the over/filtered beam can be prevented from overlapping or partially overlapping with the wavelength range of the fluorescent light, resulting in an error in measurement. [Embodiment] The detailed description and technical content of the present invention are now coordinated with the pattern. The description is as follows: Please refer to FIG. 1 and FIG. 2, which is a fluorescent generating device comprising a blue LED 10 emitting a light beam 11, a filter 20 and a fluorescent dye. 30. The filter member 20 is disposed in a light emitting direction of the blue LED 10 to receive the light beam 11 and is converted into a filtered light beam 21 having a wavelength between 465 nm and 505 nm. The dye 30 is disposed in a test tube 32, and the test tube 32 form number A0101 page 5 / 13 page M430003 is placed on the side of the filter member 20 opposite to the blue LED 1 以 to receive the filter The light beam 21 excites a fluorescent light 31, and the light spectrum is detected by a detecting module 4'. In the embodiment, the blue light diode 1〇, the light filtering member 20, and the detecting module 40 is connected to a computer 50 for controlling the luminous intensity of the blue LED 10 and the switching range of the switch, the filter 2, and receiving the information of the detecting module 40. In addition, the present invention is further provided with a heating member 60 for facilitating the progress of the PCR reaction. [0015] In general, please refer to "Figure 3", the display of the wavelength of light in the spectrum has a peak in a range and gradually decays from the peak to the sides, and the value of the wavelength only refers to The value corresponding to this peak does not mean that the light has a magnitude at a single wavelength and appears in a pulsed form. For example, the wavelength referred to in the present invention is 488 nm, which means that the peak value of the light wave is at 488 nm, and the wavelength before and after the 488 nm still has a magnitude. Therefore, if the wavelength of the light source of the blue light-emitting body 10 is not pure enough, the partial wavelength range of the light beam 11 emitted by the blue light-emitting diode 1 is likely to overlap with the wavelength of the fluorescent light 31 after the excitation, so that the detecting module 40 is detected. And receiving the light energy of the beam, causing a problem of detection error. The present invention filters the blue LED 10 through the filter 20 to obtain the filtered beam 21 to correspond to the excitation wavelength of the fluorescent dye 30. The fluorescent dye 30 can be a 6-luciferin amino group. Phosphate (6-FAM), 5-fluorescein phosphoramidate (5-FAM), Oregon Green-488 (Oregon)
Green-488 )、阿萊莎-488 (Alexa-488 )、鈣黃綠素 (Calcein)、青色素-2 (Cyanine-2)、螢光素氨基 碟酸酯（FAM)、異硫氰酸螢光素（fiuorescein iso-thiocyanate, FITC) 、 氟石螢光素 X(FluorX) 、 綠 第6頁/共13頁 表單編號A0101 色螢光蛋白（GFP)、紅位移綠色螢光蛋白（rsGFP)、 俄勒岡綠-500 (Oregon Green-500 )、若丹明-110 ( Rhodaraine 110)、若丹明綠（Rhodamine green)或 SYBR green等。其中，該濾光件20為對應「6-螢光素氨 基磷酸酯」的激發波長而調整該過濾光束21的波長為 492nm，進而得到激發後的螢光31波長於517nm處，且利 用492nm的波長進行激發「6-螢光素氨基磷酸酯」，可獲 得最佳螢光31發光效率；而若使用「5 -螢光素氨基填酸 酯」作為螢光染劑30時，該過濾光束21的波長設定為 494nm ’而得到518nm的螢光31波長；若使用「俄勒岡綠 -488」作為螢光染劑30時，該過濾光束21的波長設定為 496nm，而得到524nm的螢光31波長；若使用「阿萊莎-488」作為螢光染劑30時，該過濾光束21的波長設定為 495nm，而得到520nm的螢光31波長；若使用「鈣黃綠素 」作為螢光染劑30時，該過濾光束21的波長設定為 494nra，而得到517nm的螢光31波長；若使用「青色素-2 」作為螢光染劑30時，該過濾光束21的波長設定為 489nm，而得到506nm的螢光31波長；若使用「螢光素氨 基磷酸酯」作為螢光染劑30時，該過濾光束21的波長設 定為488nm，而得到508nm的螢光31波長；若使用「異硫 氰酸螢光素」作為螢光染劑30時，該過濾光束21的波長 設定為494nm，而得到518nm的螢光31波長；若使用「氟 石螢光素X」作為螢光染劑30時，該過濾光束21的波長設 定為494nra，而得到519nm的螢光31波長；若使用「綠色 螢光蛋白」作為螢光染劑30時，該過濾光束21的波長設 定為488nm，而得到558nm的螢光31波長；若使用「紅位 表單編號A0101 第7頁/共13頁 移綠色螢光蛋白j作為螢光染劑30時，該過濾光束21的 波長設定為488nra，而得到507nra的螢光31波長；若使用 「俄勒岡綠-500」作為螢光染劑30時，該過濾光束21的 波長設定為503nra，而得到522nm的螢光31波長；若使用 「若丹明-110」作為螢光染劑30時，該過濾光束21的波 長設定為496nm，而得到520nm的螢光31波長；若使用「 若丹明綠j作為螢光染劑30時，該過濾光束21的波長設 定為502nm，而得到527nm的螢光31波長；若使用「SY-BR green」作為螢光染劑30時，該過濾光束21的波長設 定為497nm，而得到520nm的螢光31波長。 [0016] 上述說明以精確的調整該濾光件20的過濾光束21於 特定波長範圍’進而得到最佳的螢光31激發效率。除此 之外，由於該濾光件20的濾光效果，其控制光量值於該 過濾光束21於波峰前後I5nm的波段範圍内，超過i5nra範 園之外的光量值便趨近於零，因而由上述的螢光染劑3〇 所產生的螢光31波長皆與過濾光束21的波長相距ι5ηιη以 上，而不致於有過濾光束21的波長範圍與螢光31波長重 叠造成檢測時的量測誤差問題β [0017] 氣Τ、上所述，由於本新型僅利用藍光二極體1〇進行螢 光31的激發及檢測，並透過該濾光件2〇的調整而控制該 過濾光束21的波長及頻譜範圍，進而取得最佳效率的激 發螢光31，並透過控制該濾光件2〇的過濾光束21的波長 範圍不與該螢光31的波長重疊’排除該檢測模組4〇量測 該螢光31的強度時的誤差問題。因此本新型極具進步性 及符合申請新型專利的要件，爰依法提出申請祈鈞局 表單编號Α0101 第8頁/共13頁 M430003 早曰賜准專利，實感德便。 [0018] 以上已將本新型做一詳細說明，惟以上所述者，僅 爲本新型的一較佳實施例而已，當不能限定本新型實施 的範圍。即凡依本新型申請範圍所作的均等變化與修飾 等，皆應仍屬本新型的專利涵蓋範圍内。 【圖式簡單說明】 [0019] 圖1，為本新型一較佳實施例的配置方塊示意圖。 [0020] 圖2，為本新型一較佳實施例的立體結構示意圖。Green-488), Alesha-488 (Alexa-488), Calcein, Cyanine-2, Luciferin Amino Acidate (FAM), Luciferine Isothiocyanate (fiuorescein iso-thiocyanate, FITC), Fluorescein X (FluorX), Green Page 6 of 13 Form No. A0101 Color Fluorescent Protein (GFP), Red Displacement Green Fluorescent Protein (rsGFP), Oregon Green-500 (Oregon Green-500), Rhodaraine 110, Rhodamine green or SYBR green. The filter 20 has a wavelength of 492 nm adjusted for the excitation wavelength of the "6-luciferin phosphoramidate", and the wavelength of the fluorescent 31 after excitation is 517 nm, and the wavelength of 492 nm is utilized. When the wavelength is excited to excite "6-luciferin phosphoramidate", the best fluorescent 31 luminous efficiency can be obtained. When "5-fluorescein aminourate" is used as the fluorescent dye 30, the filtered beam 21 The wavelength of the filter is set to 494 nm' to obtain a fluorescent 31 wavelength of 518 nm; if "Oregon Green-488" is used as the fluorescent dye 30, the wavelength of the filtered beam 21 is set to 496 nm, and a fluorescent 31 wavelength of 524 nm is obtained; When "Alesa-488" is used as the fluorescent dye 30, the wavelength of the filtered beam 21 is set to 495 nm, and the fluorescence 31 wavelength of 520 nm is obtained; if "calcium chlorophyll" is used as the fluorescent dye 30, The wavelength of the filtered beam 21 is set to 494 nra to obtain a fluorescence 31 wavelength of 517 nm. When "cyanine-2" is used as the fluorescent dye 30, the wavelength of the filtered beam 21 is set to 489 nm, and a 506 nm fluorescent is obtained. Light 31 wavelength; if using "luciferin amino When the phosphoric acid ester is used as the fluorescent dye 30, the wavelength of the filtered light beam 21 is set to 488 nm to obtain a fluorescence 31 wavelength of 508 nm; and when "fluorescein isothiocyanate" is used as the fluorescent dye 30, The wavelength of the filtered beam 21 is set to 494 nm to obtain a fluorescence 31 wavelength of 518 nm. When "fluorite fluorescein X" is used as the fluorescent dye 30, the wavelength of the filtered beam 21 is set to 494 nra, and 519 nm of fluorescence is obtained. 31 wavelength; if "green fluorescent protein" is used as the fluorescent dye 30, the wavelength of the filtered beam 21 is set to 488 nm, and the fluorescent wavelength of 558 nm is obtained; if "red bit form number A0101, page 7 / When 13 pages of green fluorescent protein j are used as the fluorescent dye 30, the wavelength of the filtered beam 21 is set to 488 nra, and the wavelength of the fluorescent 31 of 507 nra is obtained; if "Oregon Green-500" is used as the fluorescent dye 30 When the wavelength of the filtered beam 21 is set to 503 nra, the wavelength of the fluorescent light 31 of 522 nm is obtained. When "Rhodamine-110" is used as the fluorescent dye 30, the wavelength of the filtered beam 21 is set to 496 nm, and 520nm fluorescence 31 wavelength; if using "if When Danming Green j is used as the fluorescent dye 30, the wavelength of the filtered beam 21 is set to 502 nm to obtain a fluorescence 31 wavelength of 527 nm; and when "SY-BR green" is used as the fluorescent dye 30, the filtered beam is used. The wavelength of 21 was set to 497 nm, and a fluorescent 31 wavelength of 520 nm was obtained. [0016] The above description is to accurately adjust the filter beam 21 of the filter member 20 in a specific wavelength range to obtain an optimum fluorescence 31 excitation efficiency. In addition, due to the filtering effect of the filter member 20, the amount of control light is within the range of the wavelength band of the filter beam 21 before and after the peak of I5 nm, and the amount of light exceeding the range of i5nra is close to zero. Therefore, the wavelength of the fluorescent light 31 generated by the above-mentioned fluorescent dye 3 皆 is all above the wavelength of the filtered light beam 21, and the wavelength range of the filtered light beam 21 overlaps with the wavelength of the fluorescent light 31 to cause the amount of detection. Measuring error problem β [0017] Gas, as described above, since the present invention uses only the blue diode 1 〇 to perform excitation and detection of the fluorescent light 31, and the filtering of the filter beam 21 is controlled by the adjustment of the filter 2〇 The wavelength and the spectral range, thereby obtaining the optimum efficiency of the excitation fluorescent light 31, and the wavelength range of the filtered light beam 21 that controls the filter member 2〇 does not overlap with the wavelength of the fluorescent light 31. The detection module 4 is excluded. The error in the intensity of the fluorescent light 31 is measured. Therefore, this new type is highly progressive and meets the requirements for applying for a new type of patent. 提出Apply for the application of the praying bureau according to law Form No. 1010101 Page 8 of 13 M430003 The patent is granted as early as possible. The present invention has been described in detail above, but the above description is only a preferred embodiment of the present invention, and the scope of the present invention is not limited. That is, the equal changes and modifications made in accordance with the scope of this new application shall remain within the scope of the patent of this new type. BRIEF DESCRIPTION OF THE DRAWINGS [0019] FIG. 1 is a block diagram showing the configuration of a preferred embodiment of the present invention. 2 is a schematic perspective view of a preferred embodiment of the present invention.
[0021] 圖3，為本新型一較佳實施例的頻譜波長示意圖。 【主要元件符號說明】 [0022] 10 :藍光二極體 [0023] 11 :光束 [0024] 20 :濾光件 [0025] 21 :過濾光束3 is a schematic diagram of a spectrum wavelength of a preferred embodiment of the present invention. [Description of Main Component Symbols] [0022] 10: Blue Light Diode [0023] 11 : Light Beam [0024] 20 : Filter [0025] 21 : Filter Beam
[0026] 30 :螢光染劑 [0027] 31 :螢光 [0028] 32 :試管 [0029] 40 :檢測模組 [0030] 50 :電腦 [0031] 60 :加熱件 表單編號A0101 第9頁/共13頁30: Fluorescent dye [0027] 31 : Fluorescent [0028] 32 : Test tube [0029] 40 : Detection module [0030] 50 : Computer [0031] 60 : Heating part form number A0101 Page 9 / Total 13 pages