WO2008078526A1 - Method for detecting interaction between dna and dna-binding protein using fluorescence resonance energy transfer - Google Patents

Method for detecting interaction between dna and dna-binding protein using fluorescence resonance energy transfer Download PDF

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Publication number
WO2008078526A1
WO2008078526A1 PCT/JP2007/073559 JP2007073559W WO2008078526A1 WO 2008078526 A1 WO2008078526 A1 WO 2008078526A1 JP 2007073559 W JP2007073559 W JP 2007073559W WO 2008078526 A1 WO2008078526 A1 WO 2008078526A1
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WO
WIPO (PCT)
Prior art keywords
dna
energy transfer
resonance energy
fluorescence resonance
stranded dna
Prior art date
Application number
PCT/JP2007/073559
Other languages
French (fr)
Japanese (ja)
Inventor
Kazuhito Gohda
Original Assignee
Olympus Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corporation filed Critical Olympus Corporation
Publication of WO2008078526A1 publication Critical patent/WO2008078526A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

A method for detecting the interaction between DNA and a DNA-binding protein by measuring a change in the rate of the Brownian motion of fluorescently labeled DNA of the invention comprises the steps of: mixing a double-stranded DNA labeled with at least two types of fluorescent dyes causing fluorescence resonance energy transfer with a test sample; measuring the fluorescence intensity of a sample solution for measurement by exciting the sample solution for measurement using an excitation light with an absorption wavelength of a fluorescence resonance energy transfer donor dye and measuring the light with an emission wavelength of a fluorescence resonance energy transfer acceptor dye; and determining whether or not the rate of the motion of the double-stranded DNA in the test solution for measurement is decreased compared with the case where DNA with the same length as that of the double-stranded DNA moves alone based on a change in the fluorescence intensity over time. By measuring the fluorescence emission through fluorescence resonance energy transfer in double-stranded DNA, a measurement result which is not affected by a residue of fluorescently labeled single-stranded DNA or a substance which emits autofluorescence in a sample can be obtained.
PCT/JP2007/073559 2006-12-26 2007-12-06 Method for detecting interaction between dna and dna-binding protein using fluorescence resonance energy transfer WO2008078526A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2006-349635 2006-12-26
JP2006349635A JP2010057364A (en) 2006-12-26 2006-12-26 Method for detecting interaction between dna and dna-binding protein using fluorescence resonance energy transfer

Publications (1)

Publication Number Publication Date
WO2008078526A1 true WO2008078526A1 (en) 2008-07-03

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PCT/JP2007/073559 WO2008078526A1 (en) 2006-12-26 2007-12-06 Method for detecting interaction between dna and dna-binding protein using fluorescence resonance energy transfer

Country Status (2)

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JP (1) JP2010057364A (en)
WO (1) WO2008078526A1 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101666805A (en) * 2009-07-15 2010-03-10 苏州纳米技术与纳米仿生研究所 Method for preparing specific protein detection chip
WO2011039953A1 (en) * 2009-09-29 2011-04-07 三井造船株式会社 Method and device for fret measurement
WO2011142103A1 (en) * 2010-05-12 2011-11-17 三井造船株式会社 Fret measurement method and fret measurement device
CN112505011A (en) * 2020-12-10 2021-03-16 上海交通大学 Detection method for DNA transient conformational change based on fluorescence analysis of cyanine dyes

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012093338A (en) * 2010-09-30 2012-05-17 Fujifilm Corp Biomolecule detection device and biomolecule detection method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005519618A (en) * 2001-08-13 2005-07-07 セント・ルイス・ユニバーシテイ Rapid and sensitive proximity assay for detecting and quantifying DNA-binding proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005519618A (en) * 2001-08-13 2005-07-07 セント・ルイス・ユニバーシテイ Rapid and sensitive proximity assay for detecting and quantifying DNA-binding proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MIKUNI S. ET AL.: "Saibo Seibutsugaku ni Okeru Keiko Sokan Bunkoho to Keiko Sogo Sokan Bunkoho", PROTEIN, NUCLEIC ACID AND ENZYME, vol. 51, no. 14, 10 November 2006 (2006-11-10), pages 1998 - 2005 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101666805A (en) * 2009-07-15 2010-03-10 苏州纳米技术与纳米仿生研究所 Method for preparing specific protein detection chip
WO2011039953A1 (en) * 2009-09-29 2011-04-07 三井造船株式会社 Method and device for fret measurement
CN102597746A (en) * 2009-09-29 2012-07-18 三井造船株式会社 Method and device for FRET measurement
WO2011142103A1 (en) * 2010-05-12 2011-11-17 三井造船株式会社 Fret measurement method and fret measurement device
JP2011237342A (en) * 2010-05-12 2011-11-24 Mitsui Eng & Shipbuild Co Ltd Fret measuring method and fret measuring device
CN102792153A (en) * 2010-05-12 2012-11-21 三井造船株式会社 FRET measurement method and FRET measurement device
CN112505011A (en) * 2020-12-10 2021-03-16 上海交通大学 Detection method for DNA transient conformational change based on fluorescence analysis of cyanine dyes

Also Published As

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JP2010057364A (en) 2010-03-18

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