TWI817051B - Virus deactivation agent compositions and methods for increasing virus deactivation efficacy, and methods for virus deactivation - Google Patents

Abstract

The subject of the present invention is to provide a virus deactivation agent composition that can reduce the blending amount of ethanol as a virus deactivation component, has a high virus deactivation effect and is also excellent in safety, a method for enhancing the virus deactivation efficacy, and a virus deactivation method. . The solution of the present invention is a virus deactivating agent composition, which is selected from the group consisting of (A) 10 mass % to 60 mass % ethanol as a virus deactivating component, and (B) a virus deactivating efficacy enhancing component. One or more of fumaric acid, phosphoric acid, and citric acid are mixed with (C) water to form an acidic aqueous solution.

Description

Virus deactivation agent compositions and methods for increasing virus deactivation efficacy, and methods for virus deactivation

The present invention relates to a virus deactivation agent composition prepared by blending a virus deactivation component into an aqueous solution, a method for enhancing virus deactivation efficacy, and a virus deactivation method.

Food poisoning caused by norovirus of the family Caliciviridae has become a problem in food manufacturing and preparation facilities, medical facilities, educational facilities, etc. As methods for deactivating norovirus, methods using sodium hypochlorite solution or disinfection by hot water are known. However, it has poor usability when disinfecting with hypochlorous acid, bleaching the treated surface, or generating bad odors, and it generates chlorine gas when mixed with acidic bleach, so it is not sufficient in terms of safety. In addition, in hot water sterilization, it is difficult to maintain the desired temperature due to a temperature drop due to contact with the object to be sterilized. Furthermore, there is a risk of burns caused by hot water. Due to this current situation, virus deactivators with high safety and high virus deactivation effect are required.

In order to deactivate such viruses, virus deactivating agents blended with lower alcohols are widely used as virus deactivating ingredients. For example, Patent Document 1 discloses a virucidal agent containing at least 70% by weight of ethanol and/or propanol and 0.5 to 5% by weight of a short-chain organic acid.

Furthermore, Patent Document 2 discloses a method for deactivating virus particles, which is composed of a C1-6 alcohol selected from the group consisting of cationic oligomers or polymers, proton donors, dispersing agents, and mixtures thereof. A method for deactivating an alcohol composition that increases the amount of an enhancer in a group by contacting non-enveloped virus particles with one or more enhancers, characterized in that the alcohol composition contains a proton donor , and further contains a synergistic amount of cationic oligomer or polymer, it is also described that the alcohol concentration is 50 mass % or more.

Furthermore, Patent Document 3 discloses a disinfectant solution characterized by containing (A) 50 to 70% by weight of ethanol, and (B) at least one selected from the group consisting of organic acids, organic acid salts, and ethanolamines. 1 type 0.05~4.50% by weight, pH 6~12. [Prior technical literature] [Patent Document]

[Patent Document 1] Japanese Patent Application Publication No. Sho 63-14702 [Patent Document 2] Japanese Patent Publication No. 2009-526060 [Patent Document 3] Japanese Patent Application Publication No. 2016-210807

[Problem to be solved by the invention]

However, the virus deactivators disclosed in Patent Documents 1 to 3 all have a relatively high concentration of alcohol and have problems with flammability and irritation caused by alcohol. Therefore, it is necessary to pay attention to its use in places where fire is used, such as around the kitchen. In addition, when a bactericide containing ethanol is applied to a resin member, there is a risk that the coating or wax will peel off. This may also be a problem for people who do not like the smell of ethanol, or for people whose hands are roughened by ethanol. Use difficult problem points. Furthermore, in Patent Document 3, when the pH is 6 to 12, it is neutral to alkaline, so there is also a problem of corrosiveness due to alkali. Furthermore, since there are food, utensils, etc. around the kitchen, a highly safe virus deactivator that has no impact on health even if it adheres to these is desired.

In view of the above problems, the present invention provides a virus deactivating agent composition that can reduce the blending amount of ethanol as a virus deactivating component, has a high virus deactivating effect and is also excellent in safety, and a method for enhancing the virus deactivating efficacy, and Viral deactivation methods serve as objectives. [Means used to solve problems]

In order to achieve the above object, the present invention is a virus deactivating agent composition, which is composed of (A) 10% to 60% by mass of ethanol as a virus deactivating component, and (B) a virus deactivating efficacy enhancing component. One or more types selected from fumaric acid, phosphoric acid, and citric acid are mixed with (C) water to form an acidic aqueous solution.

Furthermore, the virus deactivating agent composition of the present invention having the above structure is characterized in that the virus deactivating efficacy enhancing component is selected from one or two types of fumaric acid and phosphoric acid.

Furthermore, the virus deactivating agent composition of the present invention having the above structure is characterized in that the virus deactivating effect enhancing component is fumaric acid.

Furthermore, the virus deactivating agent composition of the present invention having the above structure is characterized in that the blending amount of the virus deactivating efficacy enhancing component is 0.05 mass % or more.

Furthermore, the virus deactivating agent composition of the present invention having the above structure is characterized in that the blending mass ratio (A)/(B) of the aforementioned virus deactivating component and the aforementioned virus deactivating efficacy enhancing component satisfies 1.30≦log ₁₀ [ (A)/(B)]≦3.10.

Furthermore, the virus deactivating agent composition of the present invention having the above structure is characterized in that the virus deactivating component contains 10% to 40% by mass of ethanol.

Furthermore, the virus deactivating agent composition of the present invention having the above structure is characterized in that the virus deactivating component contains 30% to 60% by mass of ethanol.

Furthermore, the present invention is a method for enhancing virus deactivation efficacy, which is characterized by a virus deactivation agent composition containing (A) 10% to 60% by mass of ethanol as a virus deactivation component, and (C) water, (B) One or more ingredients selected from the group consisting of fumaric acid, phosphoric acid, and citric acid are added as a component for enhancing virus deactivation efficacy.

Furthermore, the present invention has the above-mentioned virus deactivation efficacy enhancing method, which is characterized in that the virus deactivation efficacy enhancing component is selected from one or two types of fumaric acid and phosphoric acid.

Furthermore, the present invention has the above-mentioned virus deactivation efficacy enhancing method, which is characterized in that the virus deactivation efficacy enhancing component is fumaric acid.

Furthermore, the method for enhancing virus deactivation efficacy of the present invention having the above-mentioned constitution is characterized in that the addition amount of the virus deactivation efficacy enhancing component is 0.05 mass % or more relative to the virus deactivation agent composition.

Furthermore, the present invention is a method for deactivating non-enveloped viruses, which involves contacting the non-enveloped viruses with the virus deactivating agent composition constituted above. [Effects of the invention]

According to the first structure of the present invention, the method is achieved by combining (A) 10% to 60% by mass of ethanol as a virus deactivating component, and (B) a virus deactivating efficacy enhancing component selected from the group consisting of fumaric acid and phosphoric acid. , one or more of citric acid, mixed with (C) water to form an acidic aqueous solution, and become a virus deactivating agent composition with a low ethanol concentration. According to this, there is no need to worry about flammability or skin irritation caused by ethanol or alkali, and it is a virus deactivating agent composition that has excellent usability and high virus deactivating efficacy.

Furthermore, according to the second aspect of the present invention, in the virus deactivating agent composition of the first aspect, one or two types selected from fumaric acid and phosphoric acid are used as the virus inactivating efficacy enhancing component, and It becomes a virus deactivating agent composition with high virus deactivating effect and excellent safety.

Furthermore, according to the third aspect of the present invention, the virus deactivating agent composition of the second aspect is used as a virus inactivating efficacy enhancing component, which is generally used as a chemical product and is also highly safe and easy to obtain. Acid, with a low blending amount, can more effectively improve the virus deactivation effect of ethanol, the virus deactivation ingredient, and become a virus deactivation agent composition that has both high virus deactivation effect and safety.

Furthermore, according to the fourth aspect of the present invention, in the virus deactivating agent composition having any one of the above-described first to third aspects, the blending amount of the virus deactivating efficacy enhancing component is set to 0.05 mass % or more, The blending amount of the virus deactivating efficacy enhancing component may be a blending amount necessary to fully enhance the virus deactivating efficacy of ethanol.

Furthermore, according to the fifth aspect of the present invention, by using the virus deactivating agent composition configured in any one of the first to fourth aspects, the blending mass ratio of the virus deactivating component and the virus deactivating efficacy enhancing component is ( A)/(B) is set to 1.30≦log ₁₀ [(A)/(B)]≦3.10, and the blending amount of the virus deactivating efficacy enhancing component for the virus deactivating component can be a sufficient and necessary amount.

Furthermore, according to the sixth aspect of the present invention, in the virus deactivating agent composition configured in any one of the above-mentioned first to fifth aspects, the blending amount of ethanol as the virus deactivating component is set to 10% by mass to 40% by mass. % by mass, it becomes a virus deactivator composition that further reduces the odor or irritation of ethanol and has excellent usability.

Furthermore, according to the seventh aspect of the present invention, in the virus deactivating agent composition configured in any one of the above-mentioned first to fifth aspects, the blending amount of ethanol as the virus deactivating component is set to 30% by mass to 60% by mass. Mass%, becoming a virus deactivating agent composition that further enhances the virus deactivating effect.

Furthermore, according to the eighth configuration of the present invention, by adding (B) a virus deactivating agent composition containing 10 mass % to 60 mass % ethanol as (A) virus deactivating component and (C) water. The virus deactivation potency-enhancing ingredient is selected from one or more of fumaric acid, phosphoric acid, and citric acid, which can enhance the virus deactivation potency of the virus-deactivating ingredient ethanol, making it possible to produce ethanol with a small blending amount. , a virus deactivating agent composition with high virus deactivating power.

Furthermore, according to the ninth aspect of the present invention, in the method for enhancing the virus deactivation efficacy of the eighth aspect, one or two types selected from fumaric acid and phosphoric acid are used as the virus deactivation efficacy enhancing component. A virus deactivating agent composition with higher virus deactivating efficacy and excellent safety can be produced.

Furthermore, according to the tenth aspect of the present invention, in the method for enhancing the virus deactivation effect of the ninth aspect, a commonly used chemical product, which is highly safe and easily available, is used as the virus deactivation effect enhancing component. Acid, with a low blending amount, can more effectively improve the virus deactivation effect of ethanol, a virus deactivation component, and become a virus deactivation agent composition that can be easily and cost-effectively produced with both high virus deactivation effect and safety.

Furthermore, according to the eleventh aspect of the present invention, in the method for enhancing the virus deactivation efficacy of any one of the eighth to tenth aspects, the blending amount of the virus deactivation efficacy enhancing component is set to 0.05 mass % or more, The blending amount of the virus deactivation efficacy enhancing component may be the blending amount necessary to enhance the virus deactivation efficacy of ethanol.

Furthermore, according to the twelfth aspect of the present invention, by bringing the virus deactivating agent composition of any one of the first to seventh aspects into contact with a non-enveloped virus, a non-enveloped virus with low drug susceptibility and difficulty in deactivation can be obtained. An efficient method for deactivating membrane viruses.

The virus deactivating agent composition of the present invention will be described in detail below. The virus deactivating agent composition of the present invention is composed of (A) 10 mass % to 60 mass % ethanol as a virus deactivating component, and (B) a virus deactivating efficacy enhancing component selected from specific organic acids or One or more of the inorganic acids are mixed with (C) water to form an acidic aqueous solution.

It is known that in the virus deactivating agent composition of the present invention, (A) ethanol blended as a virus deactivating component has a high virus deactivating effect. However, the present inventors discovered for the first time that the virus deactivation effect is synergistically exerted by using ethanol in combination with specific organic acids and/or inorganic acids described below.

When the blending amount of ethanol in the virus deactivating agent composition of the present invention is too small, sufficient virus deactivating effect may not be obtained. On the other hand, when the blending amount is too large, there is a problem of increased flammability or irritation.

In the virus deactivating agent composition of the present invention, as shown in the examples described below, in order to obtain sufficient virus deactivating effect and reduce the flammability or irritation caused by ethanol, ethanol is used relative to the virus deactivating agent. The total composition is blended in an amount of 10% by mass or more and 60% by mass or less. In addition, it is preferable to blend ethanol in an amount of 10% by mass to 40% by mass relative to the entire virus deactivator composition because the odor or irritation of ethanol can be further reduced. In addition, it is preferable to blend ethanol in an amount of 30% by mass to 60% by mass relative to the entire virus deactivating agent composition, since the virus deactivating effect can be further enhanced.

Specific examples of the organic acid blended into the virus deactivating agent composition of the present invention as (B) the virus deactivating efficacy enhancing component include fumaric acid and citric acid. Specific examples of inorganic acids include phosphoric acid. These organic acids and/or inorganic acids may be used alone, or two or more types may be mixed and used.

Among the above-mentioned organic acids and/or inorganic acids, especially fumaric acid and phosphoric acid, the virus deactivation effect of ethanol, the virus deactivation ingredient, can be significantly improved at a low blending amount.

The pH of the virus deactivator composition of the present invention is desirably 1 to 6. Thereby, the virus deactivating agent composition becomes acidic to weakly acidic. Compared with the alkaline virus deactivating agent composition, it is not corrosive to the skin and becomes a virus deactivating agent composition with excellent usability.

The blending amount of the virus deactivating efficacy-enhancing component of the virus deactivating agent composition of the present invention is not particularly limited. However, if the blending amount is too small, the virus deactivating efficacy compared to ethanol may not be sufficiently enhanced. possibility of effect.

As shown in the examples described below, in order to obtain a sufficient virus deactivation efficacy enhancing effect, it is preferable to blend the organic acid and/or inorganic acid of the virus deactivation efficacy enhancing component with respect to the entire virus deactivating agent composition. The blending amount is 0.05% by mass or more, more preferably 0.07% by mass or more, and still more preferably 0.1% by mass or more. Moreover, it is preferable that the organic acid and/or inorganic acid which is a virus deactivation efficacy enhancing component is blended at 5 mass % or less, more preferably 2 mass % or less, based on the entire virus deactivating agent composition, and still more preferably The blending amount is 1% by mass or less.

In the present invention, it is preferable that the blending mass ratio (A)/(B) of (A) the virus deactivating component and (B) the virus deactivating efficacy enhancing component satisfies 1.30≦log ₁₀ [(A)/(B)] ≦3.10, preferably 1.50≦log ₁₀ [(A)/(B)]≦3.00, even better still 2.10≦log ₁₀ [(A)/(B)]≦2.90.

If necessary, a surfactant can be blended into the virus deactivating agent composition of the present invention. For example, if a surfactant has the property of generating foam (foaming property), by using a surfactant with excellent foaming properties, the virus deactivator composition of the present invention can be inhibited from triggering liquid droplets when sprayed on the wall such as a spray. falls off, and the coated area becomes easily visible.

As the surfactant blended in the virus deactivating agent composition of the present invention, any one of anionic surfactants, cationic surfactants, nonionic surfactants, and amphoteric surfactants can also be suitably used. Among these, in addition to the surfactant effect, it is more suitable to use cationic surfactants that also have antiviral effects. The blending amount of the surfactant in the virus deactivating agent composition of the present invention is not particularly limited, but is preferably 0.1% by mass or more and 10% by mass or less.

Examples of anionic surfactants include fatty acid soaps, alkyl benzene sulfonates, linear alkyl benzene sulfonates, alkyl sulfates, α-olefin sulfonates, alkyl phosphate ester salts, and polyoxygenates. Ethylene alkyl ether sulfate, polyoxyethylene alkyl phenyl ether sulfate, polyoxyethylene alkyl ether phosphate, etc.

Examples of cationic surfactants include lauryltrimethylammonium chloride, benzalkonium chloride, benzethonium chloride, didecyldimethylammonium chloride, and chlorhexidine (Chlorhexidine) gluconate and other 4th-level ammonium salts.

Examples of the nonionic surfactant include polyoxyethylene alkyl ether, polyoxyethylene alkyl phenyl ether, polyoxyethylene higher fatty acid ester, polyoxyethylene sorbitol fatty acid ester, and polyoxyethylene glycerol. Fatty acid esters, polyoxyethylene hardened castor oil, coconut oil fatty acid diethanolamides, polyoxyethylene polyoxypropylene alkyl ethers, fatty acid alkanolamides, alkyl amine oxides, etc.

Examples of amphoteric surfactants include betaine-type surfactants. Specific examples include lauryl-N,N-dimethylacetate betaine, laurylamide propyl-N,N-dimethylacetate betaine, and palmitic alkylamide propyl-N,N- Dimethylhydroxypropylsulfobetaine, etc.

The virus deactivating agent composition of the present invention is aqueous type, and water is mainly used as a solvent. Examples of water include purified water such as ion exchange water or reverse osmosis membrane water, or ordinary tap water, industrial water, deep ocean water, etc.

Furthermore, in the virus deactivating agent composition of the present invention, as other components, if necessary, an inorganic antibacterial agent, an organic antibacterial agent, a virus deactivating agent, an anti-algae agent, an anti-rust agent, a solvent, a chelating agent, Fragrances, deodorizing ingredients, pH adjusters, etc. are blended in a range that does not impair the effects of the present invention to impart antibacterial effects, virus deactivation effects, anti-algae effects, anti-rust effects, cleaning effects, aromatic properties, and deodorizing effects. sex etc.

Examples of inorganic antibacterial agents, organic antibacterial agents, and virus deactivating agents include isopropylmethylphenol (IPMP), carvacrol, thymol, triclosan, and paraben. , ethyl phenyl ester, propyl phenyl ester, butyl phenyl ester, 4-chloro-3,5-dimethylphenol, o-phenylphenol, о-cresol, m-cresol, p-cresol, obtained Tebuconazole, Enilconazole, Grapefruit Seed Extract, Persimmon Seed Extract, Grape Seed Extract, Monolaurin, Monocaprin, Monocaprylin , benzoic acid, sorbic acid, glycine, alkyl diethyl aminoglycine, polylysine, dehydroacetic acid, chloramine, 3-iodo-2-propyl-N-butylaminomethyl Carbamate (IPBC), phenoxyethanol, silver zeolite, zinc pyrithione (Zinc pyrithione), thiamin lauryl sulfate, white protein, hydroxyalkyl chitosan, chitosan, etc.

Examples of algae inhibitors include sodium dichloroisocyanurate and the like. Examples of rust inhibitors include sodium benzoate and the like.

Examples of the solvent include n-paraffin, isoparaffin, flowing paraffin, naphthenic hydrocarbons, petroleum jelly, squalene, hydrocarbon solvents such as α-olefin oligomers, 1-propanol, and 2-propanol (IPA). , 1-butanol, 2-butanol, tert-butanol, 1-pentanol, 1-hexanol, benzyl alcohol, 2-phenylethanol and other alcohol solvents, 2-phenoxyethanol (ethylene glycol) Alcohol monophenyl ether), ethylene glycol, propylene glycol, 1-phenoxy-2-propanol (propylene glycol phenyl ether), 1,3-butanediol, propylene glycol monobutyl ether, dipropylene glycol monobutyl ether , glycol solvents such as tripropylene glycol monobutyl ether, etc.

Examples of fragrances include limonene such as d-limonene, pinene such as α-pinene, β-pinene, and p-cymene such as p-cymene. , indene, carotene and other hydrocarbon-based fragrances, linalool, geraniol, citronellol, l-menthol and other hydrocarbon-based fragrances, ethyllinalool, borneol, anisyl alcohol, β-phenylethyl alcohol , p-menthane-3, 8-diol, α-terpineol, γ-terpineol, terpineol, 1-hexanal, cis-3-hexen-1-ol, tetrahydrogeranol Alcohol, alcohol-based fragrances such as santalinol, cinnamyl alcohol, cedar alcohol, galaxolide, β-naphthyl methyl ether, eucalyptol alcohol, and ambroxide ), ether based fragrances such as p-cresol methyl ether, phenolic fragrances such as anethole, eugenol, isoeugenol, vanillin, ethyl vanillin, octanal, nonanal, undecane Base aldehyde, undecanal (Undecanal), decyl aldehyde, n-butyl aldehyde, isobutyl aldehyde, hexyl aldehyde, citral, citronellal, benzaldehyde, cinnamic aldehyde, anisaldehyde, cumin aldehyde, Aldehydes such as Adoxal, amyl cinnamic aldehyde, cyclamen aldehyde, etc. are fragrances, myoketone, carvone, menthone, camphor, acetophenone, butyric acid ketone, and Tonalide , α-Consonone, β-Consonone, α-MethylConsonone, β-MethylConsonone, α-isomethylConsonone, β-isomethylConsonone, γ-methyl Ketones such as cymone, γ-isomethyl cymone, turkeone, α-turkeone, β-turkeone, acetylcedrene, cashmeran, cis-jasmone, dihydrojasmone, etc. Department of fragrance, γ-butyl lactone, γ-nonalactone, γ-decalactone, γ-undecanoic acid lactone, coumarin, eucalyptol, ambrettolide, jasplactone Lactone fragrances such as (Jasmolactone), geranyl formate, octyl acetate, geranyl acetate, benzyl acetate, cinnamyl acetate, tetrahydrogeranyl acetate, menthyl acetate, agarwood acetate, propionic acid Butyl ester, benzyl acetate, methyl benzoate, allyl caproate, allyl enanthate, allyl cyclohexane propionate, allyl amyl glycolate, amyl valerianate, water Amyl acetate, isoamyl acetate, butyl acetate, ethyl butyrate, acetyl eugenol, isoamyl salicylate, allyl hexanoate, ethyl hexanoate, ethyl propionate, ethanol Ethyl acetate, methyl salicylate, citronellyl acetate, citronellyl formate, cinnamyl acetate, stearyl acetate, stearyl propionate, cedar acetate, terpine acetate Ester-based fragrances such as esters, amylcinnamaldehyde dimethyl acetal, citral dimethyl acetal and other acetal-based fragrances, indole, citrononitrile, citronellonitrile, acetaldehyde phenylethylpropyl acetic acid Ester, Tessalon, aurantiol, linalool oxide, peppermint oil, orange oil, lemon oil, lavender oil, paper peppermint oil, eucalyptus oil, citronella oil, lime oil, grapefruit Oil, jasmine oil, cypress oil, green tea essential oil, neroli oil, geranium oil, petitgrain essential oil, lemongrass oil, cinnamon oil, lemon eucalyptus oil, aphrodisiac oil, perilla oil, pine oil, rose oil, rosemary oil , camphor oil, aromatic oil, sage oil, sandalwood oil, spearmint oil, anise oil, lavender oil, oak moss oil, ocotimum oil, tiger tail oil, tonka bean tincture, turpentine oil, almond bean tincture, Basil oil, nutmeg oil, clove oil, rosewood oil, perfume tree oil, cardamom oil, cinnamon oil, cedar oil, mandarin oil, mandarin oil, anise oil, laurel oil, coriander seed oil, elemi oil, Sweet anise oil, galbanum oil, cypress oil, vetiver oil, bergamot oil, ylang-ylang oil, grapefruit oil, Siberian fir oil, ajowan oil, almond oil, angelica root oil, basil oil oil), peppermint oil, birch oil, rosewood oil, melaleuca oil, perfume tree oil, chili oil, coriander oil, celery oil, grape pomace oil, cumin oil, dill oil, tansy oil (estragon oil), garlic oil, ginger oil, hop oil, sage oil, turpentine, etc.

Examples of deodorizing ingredients include sugar cane extract, green tea extract, dried tea extract, persimmon extract, grapefruit extract, Mengzong bamboo extract, grapefruit seed extract, forsythia suspensa extract, etc. However, in addition to the deodorizing effect, sugarcane extract is suitable from the viewpoint of promoting the deactivation of norovirus.

Examples of the pH adjuster include other organic acids such as acetic acid, malic acid, and salicylic acid, other inorganic acids such as hydrochloric acid, sodium citrate, sodium carbonate, sodium bicarbonate, and sodium hydroxide.

By applying or spraying the virus deactivating agent composition of the present invention thus obtained to places where virus-infected persons are in contact, places where vomitus of virus-infected persons are handled, and places where clothes are contaminated by viruses, it can be achieved Effectively remove viruses. Furthermore, the virus deactivator composition of the present invention is extremely practical since it can be safely and easily administered by limiting the blending amount of flammable and irritating ethanol to 10 to 60% by mass.

In addition, the virus deactivation composition of the present invention has high deactivation effect on non-enveloped viruses such as norovirus, rotavirus, rhinovirus, adenovirus, etc., in addition to enveloped viruses such as influenza virus, coronavirus, and herpes virus. activation effect. According to this, conventional virus removal agents can be suitably used for the deactivation of norovirus, which is difficult to deactivate.

Furthermore, the virus deactivating agent composition of the present invention is simply a mixture of ethanol and a specific organic acid and/or inorganic acid as a virus deactivating efficacy enhancing component in water to form an acidic aqueous solution as a virus deactivating component. Very simple composition. Therefore, in addition to being easy to produce, since the ethanol concentration is as low as 60% by mass or less and is acidic, the smell of ethanol and skin irritation that are problems with most virus deactivators are also reduced, making it extremely safe. In addition, since there is no problem of discoloration caused by silver ions, the usability is also excellent.

However, the present invention is not limited to the above-described embodiments, and various modifications can be made within the scope indicated in the claims. Embodiments obtained by suitably combining the technical means disclosed in different embodiments are also included in the present invention. Technical scope. Hereinafter, the effects of the present invention will be further described in detail through examples, but the present invention is not limited to these examples. [Example 1]

[Preparation of test solution] (A) ethanol (manufactured by Wako Pure Chemical Industries, Ltd.), (B) fumaric acid, phosphoric acid, and citric acid monohydrate (the above are manufactured by Wako Pure Chemical Industries, Ltd.) are blended in the proportions shown in Table 1 and Table 3 (mass %) was blended and purified water was added to obtain a test liquid (invention 1 to 12) as 100 mass %.

Mix (A) ethanol (manufactured by Wako Pure Chemical Industries, Ltd.) (B) fumaric acid, phosphoric acid, citric acid monohydrate, lactic acid, benzoic acid, trisodium phosphate, and trisodium citrate dihydrate (the above are from Wako Pure Chemical Industries, Ltd. Co., Ltd.) and disodium fumarate (manufactured by Tokyo Chemical Industry Co., Ltd.) were blended at the blending ratios (mass %) shown in Table 2 and Table 4, and purified water was added to obtain a test solution of 100 mass %. (Comparative Examples 1 to 16). [Example 2]

[Confirmation test 1 of virus deactivation effect (Feline calicivirus)] (Preparation of test virus liquid) A cell growth medium containing 10% fetal bovine serum was added to MEM medium (manufactured by Nacalai Tesque Co., Ltd.), and CRFK cells (JCRB Cell Bank) were ) monolayer culture in tissue culture dishes. The cell proliferation medium was removed from the monolayer culture dish and inoculated with Feline calicivirus F-9 ATCC VR-782. Then, add cell maintenance medium with 2% fetal bovine serum in MEM medium, and culture in a carbonic acid gas incubator (CO ₂ concentration 5%) at 37±1°C for 1 to 5 days. Feline calicivirus is a type of non-enveloped virus and is widely used as a substitute for norovirus that cannot be cultured in cells.

After culturing, the morphology of the cells was observed using an inverted phase contrast microscope to confirm that morphological changes occurred in the cells (cell degeneration effect). Next, the culture solution was centrifuged at 1000 rpm for 3 minutes, and the supernatant obtained by ultrafine filtration was used as a test virus solution.

To 0.9 mL of the test liquids of the present invention 1 to 7 and comparative examples 1 to 11 prepared in Example 1, 0.1 mL of the test virus liquid was added and mixed to serve as an action liquid. After 1 minute, dilute the action solution 100 times with MEM culture medium to create a 10-fold dilution series. Still, add the test virus liquid to the purified water as a control, and perform the same operation.

(Measurement of virus infectivity) Using a cell growth medium, the cells to be used were cultured in a monolayer in a tissue culture microplate (96 wells), then the cell growth medium was removed and a cell maintenance medium was added every 0.1 mL. Next, 0.1 mL of a 10-fold dilution series of the action solution was inoculated into every 4 wells, and cultured in a carbonic acid gas incubator (CO ₂ concentration 5%) at 37 ± 1°C for 4 to 7 days. After culturing, use an inverted phase contrast microscope to observe the presence or absence of morphological changes (cell degeneration effect) of the cells. Calculate the 50% cell culture infection dose (TCID ₅₀ ) by the Reed-Muench method, and convert it into the infectivity value per 1 mL of the action solution. Compared with the infectivity value of purified water as a control, the logarithmic reduction value of the infectivity value was calculated.

The evaluation criteria for the virus removal effect are as follows: the logarithmic reduction of the infectious power price is 1 or less as ×, the logarithmic reduction value of the infectious power price is greater than 1 and less than 1.5 as △, and the logarithmic reduction value of the infectivity price is greater than 1.5 and less than 3 as ○ , the case greater than 3 is designated as ◎. The evaluation results of the virus infectivity value are shown in Table 1 and Table 2 together with the blending of the test solution, pH, log ₁₀ [(A)/(B)], and the log reduction value of the infectivity value.

As shown in Table 1, (A) 10% to 40% by mass of ethanol and (B) 0.05% to 0.5% by mass of fumaric acid, phosphoric acid, or citric acid monohydrate are blended. In Inventions 1 to 7 of the present invention, it was confirmed that the logarithmic reduction value of the infectivity value for feline calicivirus became larger than 1.5, indicating that it has a virus deactivation effect.

In particular, in Inventions 1 and 2, the blending amounts of ethanol are set to 40 mass% and the blending amounts of fumaric acid are set to 0.2 mass% and 0.1 mass%, respectively. When the blending amount of phosphoric acid was set to 0.05% by mass, it was confirmed that the logarithmic reduction value of the infectivity value became larger than 3, and a more significant virus deactivation effect was obtained. Furthermore, from the results of Inventions 1 to 7 and Comparative Examples 1 to 5, when ethanol was blended alone, and when fumaric acid, phosphoric acid, and citric acid monohydrate were blended alone, the logarithmic reduction value of the infectivity value was less than 1. Although No virus deactivation effect was observed, but by blending ethanol with fumaric acid, phosphoric acid, and citric acid monohydrate, it was confirmed that the virus deactivation effect was synergistically improved.

In addition, the invention 6 in which the blending amount of ethanol is 10 mass% and the blending amount of phosphoric acid is 0.5 mass%, and the comparative example in which the blending amount of ethanol is 5 mass% and the blending amount of phosphoric acid is 0.5 mass% 8, by setting the blending amount of ethanol to 10% by mass or more, it was confirmed that the virus deactivation effect was synergistically improved by blending phosphoric acid.

In addition, the invention 5 in which the blending amount of ethanol is 40 mass% and the blending amount of phosphoric acid is 0.05 mass%, and the comparative example in which the blending amount of ethanol is 40 mass% and the blending amount of phosphoric acid is 0.02 mass% 7, it was confirmed that by setting the blending amount of phosphoric acid to 0.05% by mass or more, the effect of enhancing the virus deactivation efficacy of ethanol was exhibited.

Furthermore, in Comparative Examples 9 to 11 in which 0.2% by mass of lactic acid, benzoic acid, and disodium fumarate were blended in place of fumaric acid, phosphoric acid, and citric acid monohydrate, the logarithmic decreases in infectivity were 1.5 and 0.7 respectively. , 1.33, no sufficient virus deactivation effect was observed. [Example 3]

[Confirmation test 2 of virus deactivation effect (Feline calicivirus)] (Preparation of test virus liquid) Using a cell growth medium containing 10% fetal bovine serum in MEM medium (manufactured by Nacalai Tesque Co., Ltd.), CRFK cells (JRBC cell bank) were Monolayers were cultured in tissue culture dishes. The cell proliferation medium was removed from the single-layer culture dish and inoculated with Feline calicivirus F-9 ATCC VR-782. Then, add cell maintenance medium containing MEM medium and 2% fetal calf serum, and culture in a carbonic acid gas incubator (CO ₂ concentration 5%) at 37±1°C for 1 to 5 days.

After culturing, the morphology of the cells was observed using an inverted phase contrast microscope to confirm that morphological changes occurred in the cells (cell degeneration effect). Next, the culture solution was centrifuged at 1000 rpm for 3 minutes, and the resulting supernatant was used as a virus floating solution. A 3:1 mixture of virus floating liquid and filter-sterilized 20% meat extract aqueous solution was used as the test virus liquid. The test virus liquid was used as a load and contained 5% meat extract.

To 0.9 mL of the test liquids of Invention 8 to 12 and Comparative Examples 12 to 16 prepared in Example 1, 0.1 mL of the mixed test virus liquid was added as an action liquid. After 1 minute, dilute the action solution 100 times with MEM culture medium to create a 10-fold dilution series. Still, add the test virus liquid to the purified water as a control, and perform the same operation.

The method and evaluation criteria for measuring the viral infectivity were the same as those in Example 2. The evaluation results of the virus infectivity value are shown in Table 3 and Table 4 together with the blending of the test solution, pH, log ₁₀ [(A)/(B)], and the log reduction value of the infectivity value.

As shown in Table 3, (A) 40% to 60% by mass of ethanol and (B) 0.05% to 0.2% by mass of fumaric acid, phosphoric acid, or citric acid monohydrate are blended. Inventions 8 to 12 of the present invention confirmed that the logarithmic reduction value of the infectivity of feline calicivirus became 2.5 or more, and an immediate virus deactivation effect was obtained even when loaded by meat extract.

In this regard, as shown in Table 4, in Comparative Example 12 in which 60 mass% ethanol was blended alone, and in Comparative Example 13 in which the blending amount of ethanol was 60 mass% and the blending amount of phosphoric acid was 0.02 mass%, the infectivity value was confirmed The logarithmic reduction value became less than 1, and no virus deactivation effect was observed. Furthermore, in Comparative Examples 14 and 15 in which fumaric acid and phosphoric acid were replaced by disodium fumarate and trisodium phosphate, the logarithmic reduction in infectivity was 1.43 and 1.24 respectively, indicating that no sufficient virus deactivation effect was observed. . Furthermore, in Comparative Example 15, when the pH of the test liquid is 12.51, it becomes strongly alkaline, and there is a concern about corrosiveness and roughness of hands due to alkali.

Furthermore, in Comparative Example 16, in which trisodium citrate was blended instead of citric acid monohydrate, the logarithmic decrease in infectivity value was 1.93. Although a virus deactivation effect was observed, when the pH of the test solution was 9.15, it became an alkali. properties, and worry about corrosiveness or roughness caused by alkali.

From the above results, it was confirmed that by mixing ethanol and one or two or more types selected from fumaric acid, phosphoric acid, and citric acid monohydrate with water to form an acidic aqueous solution, it has sufficient efficacy against feline calicivirus. It has a virus deactivating effect and becomes a virus deactivating agent composition with excellent safety.

Furthermore, by blending fumaric acid, phosphoric acid, and citric acid monohydrate with ethanol, it was confirmed that they act as a component that enhances the virus deactivation efficacy of ethanol.

Furthermore, although the deactivation effect on enveloped viruses by the virus deactivation composition of the present invention has not been shown here, since enveloped viruses have higher susceptibility to drugs compared with non-enveloped viruses, it goes without saying that the present invention The virus deactivation composition also has an immediate deactivation effect on enveloped viruses.

The present invention is a virus deactivation agent composition, a virus deactivation efficacy enhancement method, and a virus deactivation method that have a relatively low ethanol concentration and are excellent in acidic to weakly acidic safety, and are particularly suitable as direct Use a virus deactivator composition that is applied to virus-contaminated areas such as by spraying it.

Claims (6)

A virus deactivating agent composition in which (A) 10% to 60% by mass of ethanol as a virus deactivating component and (B) fumaric acid as a virus deactivating efficacy enhancing component are mixed in (C) water to form an acidic aqueous solution, and the added amount of the fumaric acid is 0.05% by mass or more relative to the virus deactivating agent composition. Such as the virus deactivating agent composition of claim 1, wherein the blending mass ratio (A)/(B) of the aforementioned virus deactivating component and the aforementioned virus deactivating efficacy enhancing component satisfies 1.30≦log ₁₀ [(A)/( B)]≦3.10. For example, the virus deactivating agent composition of claim 1 or claim 2, wherein the virus deactivating component contains 10 mass% to 40 mass% ethanol. For example, the virus deactivating agent composition of Claim 1 or Claim 2, wherein the virus deactivating component contains 30% to 60% by mass of ethanol. A method for enhancing virus deactivation efficacy in vitro, characterized by adding 0.05 mass to a virus deactivating agent composition containing (A) 10% to 60% by mass of ethanol and (C) water as a virus deactivating component. % or more of fumaric acid, a component that enhances the virus deactivation effect of (B). An in vitro method for deactivating non-enveloped viruses, which involves contacting non-enveloped viruses with the virus deactivating agent composition according to any one of claim 1 to claim 4.