TWI815363B - Zymosis method of shell powder - Google Patents

Abstract

The present invention relates to a zymosis method of shell powder. The method includes: azymosis step, a drying step, and a film making step. The zymosis step includes adding a shell powder into a zymosis raw material, wherein the ratio of the zymosis raw material to the shell powder is between 1:500 and 1:2000 so that the shell powder and the zymosis raw material ferments at a working temperature between 65℃ and 95℃, thereby removing the protein and minerals from the shell powder and turning the shell powder into a chitosan powder. The drying step includes separating the chitosan powder from the zymosis raw material and then drying the chitosan powder. The film making step includes performing a film making process on the dried chitosan powder and thereby tuming the chitosan powder into a chitosan film.

Description

Enzymatic hydrolysis method of carapace powder

The present invention relates to an enzymatic hydrolysis method of carapace powder, and in particular, to a method of obtaining chitosan powder by subjecting carapace powder to an enzymatic hydrolysis reaction, and a method of manufacturing a biodegradable film made of chitosan powder.

Chinese Patent Publication No. CN113372467A discloses a method for preparing chitosan by liquefying chitin, which solves the problem of environmental pollution caused by the waste liquid and residue produced by the existing high-concentration acid and alkali treatment required to prepare chitin. It goes through the following steps: 1. Chitin degradation; 2. Enzymatic hydrolysis; 3. Purification.

This invention uses squid fan-shaped bones as raw materials, soaks them in hydrogen peroxide, utilizes the explosion concentration of hydrogen peroxide, and uses controlled explosion technology to oxidatively degrade the squid fan-shaped bones to obtain degraded chitin, and then adds complex enzymes to further decompose the oxidatively degraded chitin. And impurities are removed through carbon dioxide supercritical fluid to obtain chitosan. The chitosan extraction rate of this invention process is 42%.

It uses hydrogen peroxide explosion combined with enzymatic method. In view of the steps of chitin extraction, demineralization, deproteinization, bleaching, and deacetylation, the method of obtaining chitopolymer has many shortcomings such as high pollution, excessive waste liquid, and long production process time.

Therefore, the present invention provides an enzymatic hydrolysis method of carapace powder. The steps include: an aeration step to aerate an enzymatic hydrolysis raw material. The enzymatic hydrolysis raw material contains a liquid Bacillus amylolyticum, a sugar and a deionized water, The aeration time is between 120 minutes and 180 minutes; a heating step, the enzymatic hydrolysis raw material is heated to a working temperature, the working temperature is between 65°C and 95°C; an enzymatic hydrolysis step, the enzyme The hydrolysis step is to add a shell powder to the enzymatic hydrolysis raw material. The addition ratio of the liquid Bacillus amyloides and the shell powder is between 1:500 and 1:2000. The weight of the shell powder accounts for the total amount of deionized water. The sugar accounts for between 10% and 25% of the weight percentage of the carapace powder, so that the carapace powder and the enzymatic hydrolysis raw material are fermented at the working temperature, thereby removing the carapace The proteins and minerals in the powder turn the carapace powder into a chitosan powder; a drying step separates the chitosan powder from the enzymatic raw material and dries the chitosan powder .

Further, a pulverizing step is included. The pulverizing step is to pulverize a carapace into the carapace powder before the enzymatic hydrolysis step.

Further, the carapace is selected from one of the following materials: pupa shell, black soldier fly shell, unicorn shell, cockroach shell, ladybug shell, fly shell, fish scale, shrimp shell and crab shell.

The present invention also provides a method for manufacturing a chitosan film. The steps include: a film-making step, which involves subjecting the dried chitosan powder to a film-making process, so that the chitosan powder becomes a shell. polysaccharide film.

Further, the film making process includes casting molding, coating molding, assembly molding or extrusion molding.

Further, in the film-making step, the chitosan powder contains an additive, and the additive is selected from one of the following materials or a combination thereof: citric acid, gelatin, glycerol and starch.

The present invention has the following advantages:

1. Use liquid Bacillus amyloides mixed with deionized water as the raw material for enzymatic hydrolysis to reduce environmental pollution during the enzymatic hydrolysis process and improve the enzymatic hydrolysis efficiency.

2. During the film-making process of the chitosan film, different additives are added to the chitosan powder so that the completed chitosan film has characteristics corresponding to the additives.

S1: crushing step

S2: Aeration step

S3: Heating step

S4: Enzymatic hydrolysis step

S5: Drying step

S6: Film making steps

1:Carapace powder

2: Enzymatic hydrolysis raw materials

3:Chitosan powder

4: Chitosan film

A:Substrate

B: Container

[First Figure] is a flow chart of an embodiment of the present invention.

[The second figure] is a schematic diagram of the carapace powder according to the embodiment of the present invention.

[The third figure] is a schematic diagram of the enzymatic hydrolysis raw material according to the embodiment of the present invention.

[The fourth figure] is a schematic diagram of chitosan powder according to an embodiment of the present invention.

[The fifth figure] is a schematic diagram of a chitosan film according to an embodiment of the present invention.

First, please refer to Figures 1 to 4 to illustrate the process of an embodiment of the enzymatic hydrolysis method of carapace powder of the present invention. The steps include a crushing step S1, an aeration step S2, a heating step S3, an enzymatic hydrolysis step S4, and a drying step. S5 and the forming step, the pulverizing step S1 is to pulverize the carapace by grinding or crushing, thereby pulverizing the carapace into shell powder 1 to improve the reaction efficiency of the subsequent enzymatic hydrolysis step S4.

The aeration step S2 and the heating step S3 are to aerate and heat the enzymatic hydrolysis raw material 2. In this embodiment, the enzymatic hydrolysis raw material 2 is a mixture of liquid Bacillus amyloliquefaciens, sugar and deionized water. , which has aerobic and high-temperature resistance properties. The aeration step S2 is to make the enzymatic hydrolysis raw material 2 contact the air for 120 minutes to 180 minutes before the enzymatic hydrolysis step S4, thereby completing the aeration step S2 of the enzymatic hydrolysis raw material 2. The sequence of the heating step S3 is also before the enzymatic hydrolysis step S4. It is a process of heating the enzymatic hydrolysis raw material 2 to the working temperature. The working temperature is between 65°C and 95°C. The heating step S3 enables the enzymatic hydrolysis of the raw material 2. 2 can remove minerals and proteins in the shell powder 1 in the subsequent enzymatic hydrolysis step S4, thereby converting the shell powder 1 into purified chitin, that is, chitosan powder 3.

Please continue to refer to the first to third figures to illustrate the embodiment of the enzymatic hydrolysis method of carapace powder of the present invention. The process of enzymatic hydrolysis of carapace powder 1 into chitosan powder 3 is carried out. After the carapace is pulverized step S1, obtain Carapace powder 1, the raw material of the carapace is selected from one of pupa shells, black soldier fly shells, unicorn shells, cockroach shells, ladybug shells, fly shells, fish scales, shrimp shells and crab shells. In the process, the carapace powder 1 is poured onto the plate-shaped substrate A, so that the carapace powder 1 can be poured into the container B containing the enzymatic hydrolysis raw material 2 for subsequent enzymatic hydrolysis step S4.

Enzymatic hydrolysis step S4 is to pour the carapace powder 1 into the container B containing the enzymatic hydrolysis raw material 2. The carapace powder 1 accounts for about 55% to 86% of the total weight of the deionized water in the enzymatic hydrolysis raw material 2, and the enzyme The addition ratio of liquid Bacillus amyloliquefaciens and carapace powder 1 in the enzymatic hydrolysis raw material 2 is between 1:500 and 1:2000. The sugar in the enzymatic hydrolysis raw material 2 is about 10% of the weight percentage of the carapace powder 1. ~25%, and then wait for the carapace powder 1 and the enzymatic hydrolysis raw material 2 to ferment at the working temperature, which is between 65°C and 95°C, to remove the protein and minerals in the carapace powder 1.

Please refer to Figures 1 to 4 to illustrate the embodiment of the enzymatic hydrolysis method of carapace powder of the present invention. The chitosan powder 3 is subjected to the drying step S5. Following the above, the protein in the carapace powder 1 is removed. When removing minerals, add deionized water to the enzymatic hydrolysis raw material 2 and rinse repeatedly until the pH value ranges between 6.5 and 7.5. The carapace powder 1 will be converted into chitosan powder 3, and the chitosan powder 3 will be enzymatically hydrolyzed from The raw material 2 is separated and placed on a plate-shaped substrate A to dry, and the chitosan powder 3 is deacetylated through a decomposition process.

Please refer to the first, fourth and fifth figures to illustrate the step S6 of making the dried chitosan powder 3 into a chitosan film 4 in an embodiment of the enzymatic hydrolysis method of carapace powder of the present invention. In the process, after the dry chitosan powder 3 is mixed with water, the chitosan film 4 can be formed on the surface of the substrate A through casting molding, coating molding, assembly molding or extrusion molding.

It is worth mentioning that the chitosan powder 3 can change the properties of the chitosan film 4 by adding water and additives. The water temperature ranges from 1°C to 98°C, and the chitosan powder 3 accounts for approximately the weight percentage of water. 0.01% to 15%, the additive system is selected from one or a combination of citric acid, gelatin, glycerin and starch. For example, adding citric acid can improve the moisture resistance, thermal stability and antioxidant activity of chitosan film 4. Citric acid accounts for about 0.1% to 5% of the weight of water; adding glycerin can improve the flexibility of chitosan film 4. properties, glycerol accounts for about 0.2% to 5% of the weight of water; nanosilver can also be added to increase the antibacterial activity of the chitosan film 4, nanosilver accounts for about 0.01% to 15% of the weight of water; adding oxidation Zinc increases chitosan film 4 The tensile strength of zinc oxide accounts for approximately 0.01% to 15% of the weight percent of water; adding tannic acid increases the water resistance of the chitosan film 4, and tannic acid accounts for approximately 0.01% to 15% of the weight percent of water.

Based on the description of the above embodiments, the operation, use and effects of the present invention can be fully understood. However, the above embodiments are only preferred embodiments of the present invention and should not be used to limit the implementation of the present invention. The scope, that is, simple equivalent changes and modifications based on the patent scope of the present invention and the description of the invention, are all within the scope of the present invention.

S1: crushing step

S2: Aeration step

S3: Heating step

S4: Enzymatic hydrolysis step

S5: Drying step

S6: Film making steps

Claims (3)

An enzymatic hydrolysis method of carapace powder, the steps include: an aeration step, aerating an enzymatic raw material, the enzymatic raw material contains a liquid Bacillus amylolyticus, a sugar and a deionized water, the aeration time is between Between 120 minutes and 180 minutes; a heating step, heating the enzymatic hydrolysis raw material to a working temperature, the working temperature is between 65°C and 95°C; an enzymatic hydrolysis step, the enzymatic hydrolysis step is to The carapace powder is added to the enzymatic hydrolysis raw material. The addition ratio of the liquid Bacillus amyloides and the carapace powder is between 1:500 and 1:2000. The carapace powder accounts for 55% of the total weight of the deionized water. Between 86% and 10% to 25% of the weight of the carapace powder, the sugar is allowed to ferment the carapace powder and the enzymatic raw material at the working temperature, thereby removing the protein and Minerals are used to turn the carapace powder into chitosan powder; a drying step is to separate the chitosan powder from the enzymatic hydrolysis raw material and dry the chitosan powder. The enzymatic hydrolysis method of carapace powder as described in claim 1, which includes a crushing step. The crushing step is to crush a carapace into the carapace powder before the enzymatic hydrolysis step. The enzymatic hydrolysis method of carapace powder as described in claim 2, wherein the carapace is selected from one of the following materials: pupa shell, black soldier fly shell, unicorn shell, cockroach shell, ladybug shell, fly shell, fish scale, Shrimp and crab shells.

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