TWI784725B - Tissue cell micro-cutting device - Google Patents
Tissue cell micro-cutting device Download PDFInfo
- Publication number
- TWI784725B TWI784725B TW110135474A TW110135474A TWI784725B TW I784725 B TWI784725 B TW I784725B TW 110135474 A TW110135474 A TW 110135474A TW 110135474 A TW110135474 A TW 110135474A TW I784725 B TWI784725 B TW I784725B
- Authority
- TW
- Taiwan
- Prior art keywords
- tissue
- cutting
- pushing mechanism
- sleeve
- container
- Prior art date
Links
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
Description
本發明係有關一種切割組織的裝置，特別是指一種可控制組織塊大小及長度的組織細胞微切割裝置。 The invention relates to a tissue cutting device, in particular to a tissue cell micro-cutting device capable of controlling the size and length of tissue blocks.
在生物與醫學領域中，常常會因應不同的需求進行大量組織細胞的切割，將這些切割下來的組織細胞放在玻片上進行檢驗，而這些切割完成的組織細胞需要有接近的長度與寬度，才能使實驗結果更為準確。舉例而言，將多個組織細胞分別滴上不同的測試藥品，以檢驗藥品的效果。但若組織細胞的大小不同，則難以認定實驗結果的好壞是藥品的效果還是因為組織細胞太大或太小造成的影響。 In the field of biology and medicine, a large number of tissue cells are often cut in response to different needs, and these cut tissue cells are placed on glass slides for inspection, and these cut tissue cells need to have a similar length and width. to make the experimental results more accurate. For example, a plurality of tissue cells are dripped with different test drugs to test the effect of the drugs. However, if the size of the tissue cells is different, it is difficult to determine whether the experimental results are good or bad because of the effect of the drug or because the tissue cells are too large or too small.
現階段進行上述的組織細胞切割大部分由人工進行，除了切割的組織細胞樣品其尺寸差異相當大之外，切割完成後的長型組織細胞樣品很難夾取。這樣的程序除了曠日廢時，亦造成切割的組織細胞樣品處理困難，影響實驗或檢驗的進度。 At present, most of the above-mentioned tissue cell cutting is performed manually. In addition to the considerable size difference of the cut tissue cell samples, it is difficult to clamp the long tissue cell samples after cutting. Such a procedure is not only time-consuming, but also makes it difficult to process the cut tissue cell samples, which affects the progress of experiments or inspections.
因此，本發明針對上述習知技術之缺失及未來之需求，提出一種組織細胞微切割裝置，具體架構及其實施方式將詳述於下： Therefore, the present invention proposes a tissue cell micro-dissection device for the lack of the above-mentioned conventional technology and future needs. The specific structure and its implementation will be described in detail below:
本發明之主要目的在提供一種組織細胞微切割裝置，其利用推壓機構推擠組織樣品，以控制組織塊的長度，並第一切割網的網格寬度控制組織塊的寬度，因此切割下來的組織塊尺寸完全相同，符合醫學實驗的需求。 The main purpose of the present invention is to provide a tissue cell micro-cutting device, which utilizes a pushing mechanism to push the tissue sample to control the length of the tissue block, and the grid width of the first cutting net controls the width of the tissue block, so the cut The size of the tissue blocks is exactly the same, which meets the needs of medical experiments.
本發明之另一目的在提供一種組織細胞微切割裝置，利用第一切割網和第二切割網取代刀片，同時達到切割組織樣品和過濾的目的。 Another object of the present invention is to provide a tissue cell micro-cutting device, which uses the first cutting net and the second cutting net to replace the blades, so as to achieve the purpose of cutting and filtering tissue samples at the same time.
本發明之再一目的在提供一種組織細胞微切割裝置，其在推壓機構和組織樣品之間設置墊塊，用以固定組織樣品，避免推壓機構旋轉時造成組織樣品不必要的旋轉。 Another object of the present invention is to provide a tissue cell micro-dissection device, which is provided with a spacer between the pushing mechanism and the tissue sample to fix the tissue sample and avoid unnecessary rotation of the tissue sample when the pushing mechanism rotates.
為達上述目的，本發明提供一種組織細胞微切割裝置，包括：一容器；一第一切割網，設於容器中；一推壓機構，設於第一切割網之一側，將待切割之組織樣品置於推壓機構和第一切割網之間，推壓機構將組織樣品向容器之內部推入，使組織樣品被第一切割網切割成符合第一切割網之網格寬度的複數組織塊；以及一第二切割網，設於容器中，與第一切割網貼合且位在相對於推壓機構的另一側，第二切割網旋轉以將組織塊從組織樣品上切斷。 In order to achieve the above object, the present invention provides a tissue cell micro-cutting device, comprising: a container; a first cutting net, located in the container; a pushing mechanism, located on one side of the first cutting net, to be cut The tissue sample is placed between the pushing mechanism and the first cutting net, and the pushing mechanism pushes the tissue sample into the container, so that the tissue sample is cut by the first cutting net into multiple tissues conforming to the grid width of the first cutting net block; and a second cutting net, arranged in the container, attached to the first cutting net and located on the other side relative to the pushing mechanism, the second cutting net rotates to cut off the tissue block from the tissue sample.
依據本發明之實施例，容器包括一收集器，與第二切割網相鄰，以承接被第二切割網切斷之組織塊。 According to an embodiment of the present invention, the container includes a collector adjacent to the second cutting net to receive the tissue pieces cut by the second cutting net.
依據本發明之實施例，容器上設有刻度，以供控制推壓機構進入容器的深度。 According to an embodiment of the present invention, a scale is provided on the container for controlling the depth of the pushing mechanism entering the container.
依據本發明之實施例，容器內部設有螺牙，推壓機構則設有與該螺牙對應的螺紋，使推壓機構以旋轉方式將組織樣品推入容器內部。 According to an embodiment of the present invention, the container is provided with threads, and the pushing mechanism is provided with threads corresponding to the threads, so that the pushing mechanism can push the tissue sample into the container in a rotating manner.
依據本發明之實施例，推壓機構或容器上設置一管道或管溝，而在容器或推壓機構上對應設置至少一卡點，當推壓機構沿著管道或管溝推進到卡點的位置時會卡住，再施力以使推壓機構推進到下一個卡點。 According to an embodiment of the present invention, a pipe or a trench is provided on the pushing mechanism or the container, and at least one stuck point is correspondingly set on the container or the pushing mechanism. When the pushing mechanism advances to the stuck point along the pipe or the It will be stuck when it is in the position, and then apply force to push the pushing mechanism to the next stuck point.
依據本發明之實施例，推壓機構和組織樣品之間更設有一墊塊，推壓機構推壓墊塊，使墊塊再推動組織樣品進入第一切割網。 According to an embodiment of the present invention, a pad is further provided between the pushing mechanism and the tissue sample, and the pushing mechanism pushes the pad so that the pad pushes the tissue sample into the first cutting net.
依據本發明之實施例，容器包括一第一套筒及一第二套筒，第一切割網設於第一套筒之底部，第二切割網設於第二套筒的底部，第一套筒套入第二套筒中，使第一切割網與第二切割網貼合。 According to an embodiment of the present invention, the container includes a first sleeve and a second sleeve, the first cutting net is arranged on the bottom of the first sleeve, the second cutting net is arranged on the bottom of the second sleeve, the first sleeve The sleeve is inserted into the second sleeve, so that the first cutting net and the second cutting net are bonded together.
依據本發明之實施例，推壓機構設置於第一套筒中。 According to an embodiment of the present invention, the pushing mechanism is disposed in the first sleeve.
依據本發明之實施例，第二套筒之內徑大於第一套筒之外徑，使第二套筒之筒壁包覆第一套筒之至少部份筒壁。 According to an embodiment of the present invention, the inner diameter of the second sleeve is larger than the outer diameter of the first sleeve, so that the wall of the second sleeve covers at least part of the wall of the first sleeve.
10:組織細胞微切割裝置 10: Tissue cell microdissection device
12:容器 12: container
122:第一套筒 122: The first sleeve
124:第二套筒 124: second sleeve
126:收集器 126: Collector
128:入料口 128: Feed port
14:推壓機構 14: Push mechanism
16:墊塊 16: spacer
18:第一切割網 18: First cutting mesh
20:第二切割網 20: Second cutting net
22:組織樣品 22: Tissue samples
第1圖為本發明組織細胞微切割裝置之立體圖。 Figure 1 is a perspective view of the tissue cell microdissection device of the present invention.
第2圖為本發明組織細胞微切割裝置之分解圖。 Figure 2 is an exploded view of the tissue cell microdissection device of the present invention.
第3圖為本發明組織細胞微切割裝置之側面剖視圖。 Fig. 3 is a side sectional view of the tissue cell microdissection device of the present invention.
本發明提供一種組織細胞微切割裝置，其利用第一、第二切割網進行縱向和橫向的切割，利用第一切割網的網格寬度決定組織塊的大小，因此不需要另外設置濾網，即可直接得到所需的長、寬、高尺寸的組織塊，提供後續醫療實驗時不會因組織樣品的大小不同而影響實驗結果。 The present invention provides a tissue cell micro-cutting device, which utilizes the first and second cutting nets to cut longitudinally and transversely, and utilizes the grid width of the first cutting net to determine the size of the tissue block, so additional filter nets are not required, that is, The required length, width, and height of the tissue block can be directly obtained, and the experimental results will not be affected by the size of the tissue sample when providing follow-up medical experiments.
請同時參考第1圖、第2圖及第3圖，其分別為本發明組織細胞微切割裝置10之立體圖、分解圖及側面剖視圖。本發明之組織細胞微切割裝置10包括一容器12、一推壓機構14、一墊塊16、一第一切割網18及一第二切割網20。墊塊16、第一切割網18及第二切割網20依序設於容器12中。推壓機構14與墊塊16設於第一切割網18之同一側，推壓機構14用以將待切割之組織樣品22向容器12之內部推入。第一切割網18及第二切割網20為網狀刀片或鋼絲，其平面與容器12的長度方向垂直。當組織樣品22接觸到第一切割網18後，由於組織樣品22受到另一邊的推壓機構14的推力擠壓，因此會被第一切割網18切割成符合第一切割網18之網格寬度的複數組織塊。第二切割網20與第一切割網18貼合，第二切割網旋轉以將組織塊從組織樣品上切斷。在推壓機構14和組織樣品22之間設置墊塊16的目的是為了固定組織樣品22，蓋因於推壓機構14若以旋轉方式下壓去推擠組織樣品22時，若中間沒有以墊塊16隔開，則組織樣品22即可能會被推壓機構14帶動進行不必要的旋轉，從而使切割出的組織塊變形。
Please refer to Fig. 1, Fig. 2 and Fig. 3 at the same time, which are respectively a perspective view, an exploded view and a side sectional view of the tissue
容器12的底端包括一收集器126，其與第二切割網20相鄰，用以承接被第二切割網20切斷之組織塊，且收集器126可拆卸。收集器126之底部為錐形、平面或其他形狀之凹槽，若收集器126之底部為錐形，則組織塊落入收集器126後會在傾斜表面上排列，便於夾取。此外，收集器126更可依需求注入溶液。
The bottom end of the
容器12上設有刻度，以供控制推壓機構14進入容器12的深度，例如若要控制組織塊的長度為0.5公分，就將推壓機構14推入容器12並參考刻度推入0.5公分後，旋轉第二切割網20，就可以得到長度為0.5公分的組織塊。
此外，在一實施例中，容器12內部更可設置螺牙(圖中未示)，推壓機構14則設有與螺牙對應的螺紋(圖中未示)，使推壓機構14以旋轉方式將組織樣品22推入容器12內部。或是在推壓機構14或容器12上設置管道或管溝(圖中未示)，而在容器12或推壓機構14上對應設置至少一卡點(圖中未示)，卡點的位置為預設好的推壓機構14推進距離，當推壓機構14沿著管道或管溝推進到卡點的位置時就會卡住，需稍加施力才能再推進到下一個卡點，藉由推壓機構14推進到卡點的位置以控制組織塊的長度。
The
在本發明之一實施例中，容器可分成多節，以第1~3圖為例，容器12包括一第一套筒122及一第二套筒124，收集器126則與第二套筒124相接。第一套筒122的一端為入料口128，另一端則為第一切割網18；而第二套筒124之一端為開口，另一端為第二切割網20。第二套筒124之內徑大於第一套筒122之外徑，使第二套筒124之筒壁包覆第一套筒122之至少部份筒壁，且第二套筒124可旋轉。當將第一套筒122套入第二套筒124中的時後，第一切割網18會與第二切割網20貼合。
In one embodiment of the present invention, the container can be divided into multiple sections. Taking Figures 1 to 3 as an example, the
依序將第一套筒122、第二套筒124和收集器126組裝在一起，如第1圖所示，接著將組織樣品22、墊塊16及推壓機構14依序從入料口128置入第一套筒122中。當旋轉或施力在推壓機構14時，推動墊塊16及組織樣品22向第一切割網18的方向移動。組織樣品22碰觸到第一切割網18後，由於後方還有推力，因此會擠壓第一切割網18並被第一切割網18切割成複數組織塊。當組織塊的長度達到預期時，便旋轉第二套筒124，使第二切割網20旋轉以將組織塊切斷。
Assemble the
因此，藉由本發明所提供之組織細胞微切割裝置，其利用第一切割網和第二切割網取代刀片，同時達到切割組織樣品和過濾的目的。此外，本發明利用第一切割網的網格寬度控制組織塊的寬度，再利用推壓機構推擠組織樣品的方式，控制組織塊的長度，當長度達到需求時便旋轉第二切割網將組織塊切斷，因此切割下來的組織塊尺寸完全相同，不但省略了設置濾網的必要，且相同尺寸的組織塊更符合醫學實驗對樣品一致的需求。 Therefore, with the tissue cell micro-cutting device provided by the present invention, the first cutting net and the second cutting net are used to replace the blade, and the purpose of cutting and filtering tissue samples can be achieved at the same time. In addition, the present invention uses the grid width of the first cutting net to control the width of the tissue block, and then uses the pushing mechanism to push the tissue sample to control the length of the tissue block. When the length reaches the requirement, the second cutting net is rotated to cut the tissue Therefore, the size of the cut tissue blocks is exactly the same, which not only omits the necessity of setting a filter, but also the tissue blocks of the same size are more in line with the consistent requirements of medical experiments for samples.
唯以上所述者，僅為本發明之較佳實施例而已，並非用來限定本發明實施之範圍。故即凡依本發明申請範圍所述之特徵及精神所為之均等變化或修飾，均應包括於本發明之申請專利範圍內。 The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the scope of the present invention. Therefore, all equivalent changes or modifications based on the features and spirit described in the scope of the application of the present invention shall be included in the scope of the patent application of the present invention.
10:組織細胞微切割裝置 10: Tissue cell microdissection device
12:容器 12: container
122:第一套筒 122: The first sleeve
124:第二套筒 124: second sleeve
126:收集器 126: Collector
14:推壓機構 14: Push mechanism
16:墊塊 16: spacer
18:第一切割網 18: First cutting mesh
20:第二切割網 20: Second cutting net
22:組織樣品 22: Tissue samples
Claims (9)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW110135474A TWI784725B (en) | 2021-09-24 | 2021-09-24 | Tissue cell micro-cutting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW110135474A TWI784725B (en) | 2021-09-24 | 2021-09-24 | Tissue cell micro-cutting device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TWI784725B true TWI784725B (en) | 2022-11-21 |
| TW202312942A TW202312942A (en) | 2023-04-01 |
Family
ID=85794623
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW110135474A TWI784725B (en) | 2021-09-24 | 2021-09-24 | Tissue cell micro-cutting device |
Country Status (1)
| Country | Link |
|---|---|
| TW (1) | TWI784725B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106867903A (en) * | 2017-03-30 | 2017-06-20 | 上海海洋大学 | A kind of Soft tissue cutting device for primitive cell culture |
| CN207163775U (en) * | 2017-08-08 | 2018-03-30 | 中国人民解放军第四军医大学 | For shredding the instrument of soft tissue |
-
2021
- 2021-09-24 TW TW110135474A patent/TWI784725B/en active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106867903A (en) * | 2017-03-30 | 2017-06-20 | 上海海洋大学 | A kind of Soft tissue cutting device for primitive cell culture |
| CN207163775U (en) * | 2017-08-08 | 2018-03-30 | 中国人民解放军第四军医大学 | For shredding the instrument of soft tissue |
Also Published As
| Publication number | Publication date |
|---|---|
| TW202312942A (en) | 2023-04-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4365317B2 (en) | Title of invention: Manual set for construction of tissue microarray | |
| US6241949B1 (en) | Spill-resistant microtitre trays and method of making | |
| JP2017122710A (en) | Devices, systems, and methods for extracting material from material sample | |
| TWI784725B (en) | Tissue cell micro-cutting device | |
| US9259210B2 (en) | Tissue sampling tool | |
| CN102652682A (en) | Micro brain-tissue sampler | |
| CN106596194A (en) | Time delay sampling liquid sampler and sampling method | |
| WO2019206054A1 (en) | Mass production manufacturing method for tissue chip | |
| WO2021227380A1 (en) | Preservation tube for sampling swab | |
| US20130112785A1 (en) | Methods and apparatus for sample fracturing | |
| DE19714987C1 (en) | Device for isolating particles, in particular cells | |
| CN106556526B (en) | Tumor tissue three-dimensional space sampler and sampling method thereof | |
| CN210910231U (en) | Cutting device of filter paper for it is biological experiments | |
| CN209198116U (en) | A kind of rotating disc type plant leaf blade sampler | |
| CN205462770U (en) | With multiple specification centrifuging tube assorted centrifuge rotor | |
| WO2020238602A1 (en) | Collection member and collection device | |
| CN209673460U (en) | A kind of automatic exhibition piece machine of Portable paraffin slice | |
| JP4840931B2 (en) | Substrate drying support and drying apparatus | |
| CN105496473A (en) | Novel thyroid needle biopsy device | |
| CN209264321U (en) | Soil cutting ring water suction experimental provision | |
| CN209656312U (en) | Soil sample withdrawing device in a kind of soil sample pipe | |
| CN105571912A (en) | Method for cutting flexible materials into ultrathin slices at normal temperatures | |
| JP2013088387A (en) | Sample preparation method and sample preparation device | |
| JP6770059B2 (en) | Tissue collection device | |
| CN220572214U (en) | Lung cancer tumor sampler |