TWI753593B - Novel fermented seasoning composition, processed food, and agent for improving the texture and flavor of food raw materials, and odor masking agent and method thereof - Google Patents

Novel fermented seasoning composition, processed food, and agent for improving the texture and flavor of food raw materials, and odor masking agent and method thereof Download PDF

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TWI753593B
TWI753593B TW109134181A TW109134181A TWI753593B TW I753593 B TWI753593 B TW I753593B TW 109134181 A TW109134181 A TW 109134181A TW 109134181 A TW109134181 A TW 109134181A TW I753593 B TWI753593 B TW I753593B
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lactic acid
yeast
acid bacteria
odor
fermented
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TW202108015A (en
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小川真悟
平沼未来
高野靖子
熊谷健
荒木淳也
高野茂紀
長澤淳
奥濱英則
佐野博之
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日商泰寶美客股份有限公司
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/60Drinks from legumes, e.g. lupine drinks
    • A23L11/65Soy drinks
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10Natural spices, flavouring agents or condiments; Extracts thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C21/00Whey; Whey preparations
    • A23C21/02Whey; Whey preparations containing, or treated with, microorganisms or enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/152Milk preparations; Milk powder or milk powder preparations containing additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23GCOCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G1/00Cocoa; Cocoa products, e.g. chocolate; Substitutes therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23GCOCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G1/00Cocoa; Cocoa products, e.g. chocolate; Substitutes therefor
    • A23G1/30Cocoa products, e.g. chocolate; Substitutes therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/14Vegetable proteins
    • A23J3/16Vegetable proteins from soybean
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/50Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/70Germinated pulse products, e.g. from soy bean sprouts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L23/00Soups; Sauces; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/24Synthetic spices, flavouring agents or condiments prepared by fermentation
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/36Freezing; Subsequent thawing; Cooling
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification

Abstract

本發明改良加熱調理後經冷卻而冷藏或冷凍之冷藏食品或冷凍食品之素材感(係指明顯地感覺到食材所特有之味道及/或食感(質地))。本發明之課題在於提供一種用於畜肉等之軟化、豆乳之腥味之遮蔽、植物蛋白質臭之遮蔽、香辛料之刺激感之增強、可可風味提昇、牛乳或豆乳之光劣化臭之抑制、乳感提昇、味噌之穀物風味之提昇、魚肉臭遮蔽、畜肉臭遮蔽、果實感提昇、或納豆臭遮蔽之劑。本發明係基於乳清或豆乳之利用乳酸菌及酵母所產生之醱酵物(乳酸菌/酵母醱酵物)。較佳為基於利用酵母使乳清或豆乳之乳酸菌醱酵物進一步醱酵而獲得之乳酸菌/酵母醱酵物。 The present invention improves the texture of refrigerated or frozen foods that have been cooled and refrigerated or frozen after heating and conditioning (referring to clearly feeling the unique taste and/or texture (texture) of the ingredients). The object of the present invention is to provide a method for softening livestock meat, etc., shielding the fishy smell of soybean milk, shielding the odor of vegetable protein, enhancing the pungent feeling of spices, enhancing the flavor of cocoa, suppressing the light deterioration odor of milk or soybean milk, and milky feeling. Enhancement, Miso Grain Flavor Enhancement, Fish Smell Masking, Livestock Smell Masking, Fruity Flavor Enhancement, or Natto Smell Masking Agent. The present invention is based on a ferment (lactic acid bacteria/yeast ferment) produced by using lactic acid bacteria and yeast of whey or soymilk. Preferably, it is a lactic acid bacteria/yeast starter obtained by further fermenting a lactic acid bacteria starter of whey or soymilk with yeast.

Description

新穎發酵調味料組合物、加工食品、與用於食品原料之素材感及風味之提昇、臭味遮蔽之劑及其方法 Novel fermented seasoning composition, processed food, and agent for improving the texture and flavor of food raw materials, and odor masking agent and method thereof

本發明係關於一種新穎之醱酵調味料組合物。更詳細而言,係關於一種使用有利用乳酸菌及酵母所產生之醱酵物之用以改良冷凍食品等中之食材之素材感之醱酵調味料組合物。本發明係於食品製造等領域中有用。 The present invention relates to a novel fermented seasoning composition. More specifically, it relates to a fermented seasoning composition for improving the texture of ingredients in frozen foods and the like using fermented products produced by lactic acid bacteria and yeast. The present invention is useful in the fields of food production and the like.

冷凍燒賣、冷凍餃子、冷凍春捲等調理冷凍食品存在如下優點,即可儲購‧保存,亦不會產生於調理中產生之材料之廢棄部或浪費,以短時間之調理增加餐桌或便當之菜品,且容易配色等。近年來,就使味道或風味變優異之觀點而言,亦使用有各種技術,並廣泛應用於包括普通家庭之各種情景。 Frozen shumai, frozen dumplings, frozen spring rolls and other prepared frozen foods have the following advantages: they can be stored, purchased and preserved, and there will be no waste or waste of materials generated in the preparation process, so that the dishes on the table or lunch boxes can be increased by a short period of preparation. , and easy color matching. In recent years, from the viewpoint of improving the taste or flavor, various techniques are also used, and they are widely used in various situations including ordinary households.

另一方面,為了提高食品之味道或風味,而添加胺基酸系調味料、核酸系調味料、或者以穀類、果實、蔬菜或乳製品等為主原料之醱酵調味料。關於醱酵調味料,提出有一些以乳清或豆乳為原料者。關於乳清,例如專利文獻1及2係提出特徵在於含有藉由乳酸菌使乳清醱酵,繼而藉由酵母使之醱酵而獲得之澄清乳酸菌/酵母乳清醱酵液作為有效成分之調味液風味改良劑、及果實飲料之風味增強劑。又,專利文獻3係提出特徵在於對自醱酵乳將凝乳去除而獲得之醱酵乳乳清進行酵母醱酵之醱酵乳乳清醱酵液之製造法、及使用所獲得之醱酵乳乳清醱酵液作為飲料或調味料之醱酵風味賦予劑之有效成分的情況。On the other hand, in order to improve the taste or flavor of food, amino acid-based seasonings, nucleic acid-based seasonings, or fermented seasonings based on cereals, fruits, vegetables, or dairy products are added. Regarding the fermented seasoning, it is proposed that some use whey or soy milk as the raw material. Regarding whey, for example, Patent Documents 1 and 2 propose seasoning liquids characterized by containing, as active ingredients, clarified lactic acid bacteria/yeast whey fermented liquid obtained by fermenting whey with lactic acid bacteria and then fermenting it with yeast Flavor improver, and flavor enhancer for fruit beverages. In addition, Patent Document 3 proposes a method for producing a fermented whey fermented liquid characterized by performing yeast fermentation on fermented whey obtained by removing curd from fermented milk, and using the obtained fermented whey. The case where the yeast liquid is used as the active ingredient of the fermented flavor imparting agent of the beverage or seasoning.

關於乳清之醱酵物,雖並非以調味料之形式提出,但專利文獻4係提出含有血管收縮素轉化酶抑制肽之醱酵乳之製造法作為可以高回收率有效率地獲得以較高比例包含安全性較高而可用作醫藥品、功能性食品、健康食品等之ACE肽之醱酵乳及乳清的製造法,該製造法包含(A)將乳酸菌與包含奶之原料進行混合攪拌而獲得混合原料之步驟;(B-1)使上述混合原料於攪拌下進行醱酵以使凝乳碎片、與包含血管收縮素轉化酶抑制肽之乳清生成的步驟,而調製含有凝乳碎片、與包含血管收縮素轉化酶抑制肽之乳清之醱酵乳。 關於豆乳,例如專利文獻5係提出如下使用有大豆之調味料之製造方法,該製造方法之特徵在於:將大豆磨碎而分離為豆乳組分與豆腐渣組分,將該豆乳組分與豆腐渣組分以任意之比例進行調配,對藉由該調配而獲得之混合物進行酶處理而製造調味料。再者,雖並非豆乳,但專利文獻15係提出如下食品之異味・異臭抑制組合物,該組合物之特徵在於:將於食鹽5重量%以下或無鹽之狀態下藉由真菌、酵母及乳酸菌中之任一種1種以上使大豆醱酵分解而獲得之大豆醱酵物設為主成分。 又,雖並非以調味料之形式提出,但專利文獻6係提出如下豆乳醱酵酒之製造方法作為沒有腥味或澀味,進而香味濃郁,發揮源自豆乳之風味之豆乳醱酵酒的製造方法,該製造方法之特徵在於:於酒類之製造步驟中,即將醱酵前之醪糟含有豆乳,向該醪糟添加酵母或酵母與乳酸菌而使之醱酵。專利文獻7係提供如下醱酵豆乳之製造法,其特徵在於:藉由乳酸菌及酵母菌,對作為沒有大豆臭且風味良好之適合大眾之豆乳之添加有糖類或未添加有糖類的豆乳進行酒精醱酵及乳酸醱酵而獲得醱酵豆乳,對所獲得之醱酵豆乳進行菌之惰性化處理及將二氧化碳與酒精一併去除之處理。進而,專利文獻8係提出如下免疫增強劑,其係以將乳酸菌與酵母菌進行共棲培養,使大豆或其加工物進行醱酵而獲得之醱酵物作為有效成分。 [先前技術文獻] [專利文獻] [專利文獻1]日本專利特開平7-75520號公報(日本專利第3333011號) [專利文獻2]日本專利特開平7-75521號公報(日本專利第3343790號) [專利文獻3]日本專利特開2004-236638號公報 [專利文獻4]國際公開WO00/41572 [專利文獻5]日本專利特開2003-24003號公報 [專利文獻6]日本專利特開昭59-169483號公報 [專利文獻7]日本專利特開平11-46685號公報 [專利文獻8]日本專利特開2003-335695號公報 [專利文獻9]日本專利特開平4-36167號公報 [專利文獻10]日本專利特開2007-319166號公報 [專利文獻11]日本專利特開2003-304836號公報 [專利文獻12]國際公開WO2010/126165 [專利文獻13]日本專利特開2002-281942號公報 [專利文獻14]日本專利特開2010-166886號公報 [專利文獻15]日本專利特開2007-129985號公報 [專利文獻16]日本專利特開2011-217646號公報 [專利文獻17]日本專利特開2012-175963號公報 [專利文獻18]日本專利特開2010-200635號公報 [專利文獻19]日本專利特開昭60-203174號公報 [專利文獻20]日本專利特開2011-4673號公報 [專利文獻21]國際公開WO2013/031571 [專利文獻22]日本專利特開2009-261385號公報 [專利文獻23]日本專利特開2006-61066號公報 [專利文獻24]日本專利特開2010-57434號公報 [專利文獻25]日本專利特開2015-216846號公報 [專利文獻26]日本專利特開2016-42818號公報 [專利文獻27]日本專利特開2009-297009號公報 [專利文獻28]日本專利特開2001-299267號公報 [專利文獻29]日本專利特開2015-43749號公報 [專利文獻30]日本專利特開2011-254762號公報 [專利文獻31]國際公開WO2011/074359 [專利文獻32]日本專利特開2010-233460號公報 [專利文獻33]日本專利特開2009-22266號公報 [專利文獻34]日本專利特開2007-97587號公報 [專利文獻35]日本專利特開2003-284528公報 [專利文獻36]日本專利特開2003-289836公報Although the fermented product of whey is not proposed in the form of seasoning, Patent Document 4 proposes a method for producing fermented milk containing angiotensin-converting enzyme inhibitory peptide, which can be efficiently obtained at a high recovery rate. A method for producing fermented milk and whey that are highly safe and can be used as ACE peptides in pharmaceuticals, functional foods, health foods, etc., the production method comprising (A) mixing and stirring lactic acid bacteria and a raw material containing milk to obtain The step of mixing raw materials; (B-1) the step of fermenting the above mixed raw materials under stirring to produce curd fragments and whey containing angiotensin-converting enzyme inhibitory peptide, and preparing curd fragments, and A fermented milk of whey containing angiotensin-converting enzyme inhibitory peptide. Regarding soymilk, for example, Patent Document 5 proposes a method for producing a seasoning using soybeans, which is characterized in that soybeans are ground and separated into a soymilk component and a bean curd okara component, and the soymilk component and bean curd are separated. The slag component is prepared in an arbitrary ratio, and the mixture obtained by the preparation is subjected to enzyme treatment to manufacture a seasoning. Furthermore, although it is not soymilk, Patent Document 15 proposes a composition for suppressing odor and odor of food, which is characterized in that fungi, yeast and lactic acid bacteria are used in a state of 5% by weight or less of salt or without salt. One or more kinds of soybean fermented products obtained by decomposing soybean fermented soybeans are used as the main component. Also, although it is not proposed in the form of seasonings, Patent Document 6 proposes the following production method of soymilk fermented wine as the production of soymilk fermented wine which has no fishy smell or astringency, and has a strong fragrance and exerts the flavor derived from soymilk. The manufacturing method is characterized in that: in the manufacturing step of alcoholic beverages, the moromi before fermentation contains soybean milk, and yeast or yeast and lactic acid bacteria are added to the moromi to ferment. Patent Document 7 provides a method for producing fermented soymilk, which is characterized in that sugar-added or sugar-free soymilk, which is soymilk with no soy odor and good taste, which is suitable for the general public, is subjected to alcohol treatment with lactic acid bacteria and yeast. The fermented soymilk is obtained by fermenting and lactic acid fermentation, and the obtained fermented soymilk is subjected to a process of inertizing bacteria and removing carbon dioxide and alcohol together. Furthermore, Patent Document 8 proposes an immunopotentiator using, as an active ingredient, a fermented product obtained by symbiotically culturing lactic acid bacteria and yeast, and fermenting soybeans or processed products thereof. [Prior Art Literature] [Patent Literature] [Patent Document 1] Japanese Patent Laid-Open No. 7-75520 (Japanese Patent No. 3333011) [Patent Document 2] Japanese Patent Laid-Open No. 7-75521 (Japanese Patent No. 3343790) [Patent Document 3] Japanese Patent Laid-Open No. 2004-236638 [Patent Document 4] International Publication WO00/41572 [Patent Document 5] Japanese Patent Laid-Open No. 2003-24003 [Patent Document 6] Japanese Patent Laid-Open No. 59-169483 [Patent Document 7] Japanese Patent Laid-Open No. 11-46685 [Patent Document 8] Japanese Patent Laid-Open No. 2003-335695 [Patent Document 9] Japanese Patent Laid-Open No. 4-36167 [Patent Document 10] Japanese Patent Laid-Open No. 2007-319166 [Patent Document 11] Japanese Patent Laid-Open No. 2003-304836 [Patent Document 12] International Publication WO2010/126165 [Patent Document 13] Japanese Patent Laid-Open No. 2002-281942 [Patent Document 14] Japanese Patent Laid-Open No. 2010-166886 [Patent Document 15] Japanese Patent Laid-Open No. 2007-129985 [Patent Document 16] Japanese Patent Laid-Open No. 2011-217646 [Patent Document 17] Japanese Patent Laid-Open No. 2012-175963 [Patent Document 18] Japanese Patent Laid-Open No. 2010-200635 [Patent Document 19] Japanese Patent Laid-Open No. 60-203174 [Patent Document 20] Japanese Patent Laid-Open No. 2011-4673 [Patent Document 21] International Publication WO2013/031571 [Patent Document 22] Japanese Patent Laid-Open No. 2009-261385 [Patent Document 23] Japanese Patent Laid-Open No. 2006-61066 [Patent Document 24] Japanese Patent Laid-Open No. 2010-57434 [Patent Document 25] Japanese Patent Laid-Open No. 2015-216846 [Patent Document 26] Japanese Patent Laid-Open No. 2016-42818 [Patent Document 27] Japanese Patent Laid-Open No. 2009-297009 [Patent Document 28] Japanese Patent Laid-Open No. 2001-299267 [Patent Document 29] Japanese Patent Laid-Open No. 2015-43749 [Patent Document 30] Japanese Patent Laid-Open No. 2011-254762 [Patent Document 31] International Publication WO2011/074359 [Patent Document 32] Japanese Patent Laid-Open No. 2010-233460 [Patent Document 33] Japanese Patent Laid-Open No. 2009-22266 [Patent Document 34] Japanese Patent Laid-Open No. 2007-97587 [Patent Document 35] Japanese Patent Laid-Open No. 2003-284528 [Patent Document 36] Japanese Patent Laid-Open No. 2003-289836

[發明所欲解決之問題] 冷凍・冷藏食品係使用各種各類之素材作為原料。例如,冷凍春捲係使用筍、香菇、捲心菜、胡蘿蔔、豬肉等。該等食材係於加熱調理時出來味道,進行調和而作為食品整體之整體感增強,另一方面,若進行冷卻並於低溫下進行保存,則大大有損各素材之特有之味道或食感,而於食用時於口中變得難以判別各素材。 另一方面,針對畜肉、魚肉或該等之加工品,主要對食感之改善進行有研究。其中,關於畜肉等之軟化之技術係對乳幼兒飲食、醫院飲食、護理飲食等而言重要,已知有添加鹼製劑之方法(專利文獻9);添加蛋白質分解酶之方法(專利文獻10);添加焙過之精鹽、糖醇、澱粉衍生物及有機酸之方法(專利文獻11);添加乳酸鈣、胺基酸及糖質之方法(專利文獻12);添加海藻酸酯之方法(專利文獻13)等。 又,因過氧酸銨或卡路里之過量攝取等問題,故而對使用豆乳或植物蛋白質代替牛乳或畜肉等之情況進行有研究,但有豆乳之腥味或植物蛋白質之獨特氣味之顧慮。另一方面,對為了增強香辛料等之香味而使用特定之源自酵母之酵母萃取物之情況進行有研究(專利文獻14)。 進而,關於巧克力之味道及風味之改善,研究有:使用乳清礦物質而使巧克力風味能夠強烈地感覺到之情況(專利文獻16);以含有大量麩胺酸且亦包含較多核酸類之酵母萃取物為有效成分之巧克力製品之食感改善劑(專利文獻17)。關於乳製品,提出有乳製品之光劣化臭之掩蔽劑,其特徵在於:含有選自由1,3-辛二醇、5-辛烯-1,3-二醇及二甲基甲氧基呋喃酮所組成之群中之化合物作為有效成分(專利文獻18);含有奶之酸性飲料之製造法,其特徵在於:添加芸香苷、桑色素或檞皮酮中之1種或2種以上(專利文獻19)。關於濃厚之奶味、類似牛乳之濃郁感或甜味,研究有:藉由使用特定之酵母萃取物而增強濃厚之奶味或奶之濃郁感(專利文獻20、21);調配有將食用植物油脂與酵母萃取物粉末之混合物以粉末狀態進行加熱而製備之具有類似肉之風味之粉末調味料與酵母萃取物的乳酪風味及/或乳感增強劑(專利文獻22);將果汁與酵母萃取物進行混合並加熱而成之用以改善乳製品之風味之調味料(專利文獻23);將源自從啤酒醸造步驟回收之酵母之脫苦味乾燥酵母為主要成分之含奶食品用乳感增強劑(專利文獻24)。關於以大豆為原料素材之食品,對包含豆乳之脂肪酶處理物之大豆風味賦予劑進行有研究(專利文獻25)。關於使用有魚肉及畜肉之食品,研究有:使用經同時對酒糟進行澱粉分解酶處理及蛋白質分解酶處理,並利用酵母使之再醱酵之步驟而獲得之萃取物的沙丁魚或雞肉之腥味之遮蔽(專利文獻26);利用使豆乳進行乳酸醱酵而獲得之乳酸菌生成萃取物之鯊魚肉之臭味抑制(專利文獻27);將水產動物類之萃取物設為主原料並利用麴黴進行處理,進而添加乳酸菌及酵母,使醪糟進行醱酵熟成而獲得之先前之萃取物或魚醬油之異味得到減少之醱酵調味料(專利文獻28)。又,研究有:含有自利用綠麴菌使包含大豆之原料醱酵而獲得之醱酵分解物獲得之水溶性多糖作為有效成分的畜肉用消臭組合物(專利文獻29);包括向包含牛肉之肉塊添加精胺酸之步驟之畜臭得到遮蔽之牛肉或牛肉加工食品之製造方法(專利文獻30);利用相對於全部固形物成分含有5質量%以上之精胺酸而成之酵母萃取物組合物之畜肉臭的抑制及/或目標味道之增強(專利文獻31);食材之處理方法,其特徵在於:藉由使白酒麴清酒或白酒麴甜料酒與畜肉或水產動物類進行接觸而抑制醛之產生,從而減少或抑制上述畜肉或水產動物類之異味(專利文獻32);將烏頭酸之含量為1 mg/L以上且3000 mg/L以下之甜料酒類添加至煮汁中而對畜肉進行加熱調理,藉此一面抑制畜肉之氣味一面賦予濃郁味道之方法(專利文獻33);應用有內酯類及/或含內酯類之物質之青草臭得到改善之牛肉或加工牛肉製品之製造方法(專利文獻34);利用以包含特定量之5'-核苷酸類、β-葡聚糖、甘露聚醣、及肽之酵母萃取物為有效成分之畜肉食品之肉色改善劑的獸臭之遮蔽(專利文獻35)。關於含果汁之飲料,研究有以具有內分支環狀結構部分與外分支結構部分,且聚合度為50以上之葡聚糖為有效成分之果汁感提昇劑(專利文獻36)。 然而,未對以該等目的而使用以乳清或豆乳為原料之醱酵調味料之情況進行研究。 [解決問題之技術手段] 本發明者等人對澄清乳酸菌/酵母乳清醱酵液進行了各種研究(參照專利文獻1、2)。其中,關於添加有乳清或豆乳之利用乳酸菌與酵母之醱酵液並經調理之冷凍食品,發現存在較先前,食材之素材感得到維持之傾向。又,發現乳清或豆乳之利用乳酸菌與酵母之醱酵液可用於畜肉等之軟化、豆乳之腥味之遮蔽、植物蛋白質臭之遮蔽、香辛料之刺激感之增強。進而發現乳清或豆乳之利用乳酸菌與酵母之醱酵液可用以可可風味提昇、牛乳或豆乳之光劣化臭之抑制、乳感提昇、味噌之穀物風味之提昇、魚肉臭遮蔽、畜肉臭遮蔽、果實感提昇、及納豆臭遮蔽。基於該等見解,而完成本發明。 即,本發明係提供以下。 [1]一種醱酵調味料組合物,其係以豆乳或乳清之利用乳酸菌及酵母所產生之醱酵物(乳酸菌/酵母醱酵物)為有效成分之用於選自由食品之素材感改良、畜肉或魚肉之軟化、豆乳之腥味遮蔽、植物蛋白質臭遮蔽、香辛料之刺激之增強、可可風味提昇、牛乳或豆乳之光劣化臭之抑制、乳感提昇、味噌之穀物風味之提昇、魚肉臭遮蔽、畜肉臭遮蔽、果實感提昇、及納豆臭遮蔽所組成之群中之任一目的者。 [2]如1記載之醱酵調味料組合物,其中乳酸菌為選自由鼠李糖乳酸桿菌(Lactobacillus rhamnosus)、乳酸小球菌(Pediococcus acidlactici)、融合乳酸桿菌(Lactobacillus confusus)、壞醱酵乳酸桿菌(Lactobacillus malefermentans)、副乾酪乳酸桿菌(Lactobacillus paracasei subsp. paracasei)、保加利亞乳酸桿菌(Lactobacillus delbrueckii subsp. bulgaricus)、乳酸乳球菌(Lactococcus lactis)、嗜酸乳酸桿菌(Lactobacillus acidophilus)、及瑞士乳酸桿菌(Lactobacillus helveticus)所組成之群中之任一種。 [3]如1或2記載之醱酵調味料組合物,其中酵母為選自由釀酒酵母菌(Saccharomyces cerevisiae)、清酒酵母菌(Saccharomyces sake)、白葡萄酒酵母菌(Saccharomyces beticus)、粟酒裂殖酵母菌(Schizosaccharomyces pombe)所組成之群中之任一種。 [4]如1至3中任一項記載之醱酵調味料組合物,其以豆乳之乳酸菌/酵母醱酵物作為有效成分。 [5]一種食品,其包含1至4中任一項中所定義之乳酸菌/酵母醱酵物之有效量。 [6]一種經加熱調理之冷藏食品或冷凍食品,其包含1至4中任一項中所定義之乳酸菌/酵母醱酵物之有效量。 [7]一種如5記載之食品、或如6記載之冷藏食品或冷凍食品,其於為以乳清為原料之乳酸菌/酵母醱酵物之情形時,換算為固形物成分10.7%之乳酸菌/酵母醱酵物之乳酸菌/酵母醱酵物,於為以豆乳為原料之乳酸菌/酵母醱酵物之情形時,換算為固形物成分6.5%之乳酸菌/酵母醱酵物而包含0.1~2.0%之乳酸菌/酵母醱酵物。 [8]一種冷藏食品或冷凍食品之製造方法,其包括如下步驟:將包含1至4中任一項中所定義之乳酸菌/酵母醱酵物之有效量的經加熱調理之食品進行冷藏或冷凍而獲得冷藏食品或冷凍食品;或者 一種於原料中包含畜肉或魚肉、豆乳、植物蛋白質、香辛料、源自可可之原料、牛乳、味噌、番茄或果實、或者納豆之食品之製造方法,其包括如下步驟:添加1至4中任一項中所定義之乳酸菌/酵母醱酵物之有效量。 [9]一種冷藏食品或冷凍食品之素材感之改良方法,其包括如下步驟:將包含1至4中任一項中所定義之乳酸菌/酵母醱酵物之有效量的經加熱調理之食品進行冷藏或冷凍而獲得冷藏食品或冷凍食品;或者 一種用於選自由畜肉或魚肉之軟化、豆乳之腥味遮蔽、植物蛋白質臭遮蔽、或香辛料之刺激之增強、可可風味提昇、牛乳或豆乳之光劣化臭之抑制、乳感提昇、味噌之穀物風味之提昇、魚肉臭遮蔽、畜肉臭遮蔽、果實感提昇、及納豆臭遮蔽所組成之群中之任一目的之方法,其包括如下步驟:添加1至4中任一項中所定義之乳酸菌/酵母醱酵物之有效量。 [10]一種以乳酸菌/酵母醱酵物作為有效成分之醱酵調味料組合物之製造方法,其包括如下步驟:利用酵母使豆乳或乳清之乳酸菌醱酵物進一步醱酵。 [11]如1至4中任一項記載之醱酵調味料組合物,其中有效成分包含基於乳酸菌/酵母醱酵物之分子量而區分之組分,且組分之下限之分子量為75~1,800,上限之分子量為2,200~10,000。 [12]一種用於選自由食品之素材感改良、畜肉或魚肉之軟化、豆乳之腥味遮蔽、植物蛋白質臭遮蔽、香辛料之刺激之增強、可可風味提昇、牛乳或豆乳之光劣化臭之抑制、乳感提昇、味噌之穀物風味之提昇、魚肉臭遮蔽、畜肉臭遮蔽、果實感提昇、及納豆臭遮蔽所組成之群中之任一目的之劑,其包含豆乳或乳清之乳酸菌/酵母醱酵物之分子量500~3,500之組分。 [1']一種豆乳或乳清之利用乳酸菌及酵母所產生之醱酵物(乳酸菌/酵母醱酵物)之用途,其用於選自由食品之素材感改良、畜肉或魚肉之軟化、豆乳之腥味遮蔽、植物蛋白質臭遮蔽、香辛料之刺激之增強、可可風味提昇、牛乳或豆乳之光劣化臭之抑制、乳感提昇、味噌之穀物風味之提昇、魚肉臭遮蔽、畜肉臭遮蔽、果實感提昇、及納豆臭遮蔽所組成之群中之任一目的。 [2']如1'記載之用途,其中乳酸菌為選自由鼠李糖乳酸桿菌(Lactobacillus rhamnosus)、乳酸小球菌(Pediococcus acidlactici)、融合乳酸桿菌(Lactobacillus confusus)、壞醱酵乳酸桿菌(Lactobacillus malefermentans)副乾酪乳酸桿菌(Lactobacillus paracasei subsp. paracasei)、保加利亞乳酸桿菌(Lactobacillus delbrueckii subsp. bulgaricus)、乳酸乳球菌(Lactococcus lactis)、嗜酸乳酸桿菌(Lactobacillus acidophilus)、及瑞士乳酸桿菌(Lactobacillus helveticus)所組成之群中之任一種。 [3']如1'或2'記載之用途,其中酵母為選自由釀酒酵母菌(Saccharomyces cerevisiae)、清酒酵母菌(Saccharomyces sake)、白葡萄酒酵母菌(Saccharomyces beticus)、粟酒裂殖酵母菌(Schizosaccharomyces pombe)所組成之群中之任一種。 [4']如1'至3'中任一項記載之用途,其以豆乳之乳酸菌/酵母醱酵物作為有效成分。 [5']如1'至4'中任一項記載之用途,其中有效成分包含基於乳酸菌/酵母醱酵物之分子量而區分之組分,且組分之下限之分子量為75~1,800,上限之分子量為2,200~10,000。 [6']一種豆乳或乳清之乳酸菌/酵母醱酵物之分子量500~3,500之組分之用途,其用於選自由食品之素材感改良、畜肉或魚肉之軟化、豆乳之腥味遮蔽、植物蛋白質臭遮蔽、香辛料之刺激之增強、可可風味提昇、牛乳或豆乳之光劣化臭之抑制、乳感提昇、味噌之穀物風味之提昇、魚肉臭遮蔽、畜肉臭遮蔽、果實感提昇、及納豆臭遮蔽所組成之群中之任一目的。[Problems to be Solved by Invention] Frozen and refrigerated foods use a variety of materials as raw materials. For example, frozen spring rolls use bamboo shoots, shiitake mushrooms, cabbage, carrots, pork, and the like. These ingredients are flavored during heating and conditioning, and they are blended to enhance the overall sense of food as a whole. On the other hand, if they are cooled and stored at low temperatures, the unique taste or texture of each ingredient will be greatly impaired. When eating, it becomes difficult to distinguish each ingredient in the mouth. On the other hand, with regard to animal meat, fish meat or processed products thereof, researches are mainly conducted on the improvement of food texture. Among them, techniques for softening animal meat are important for infant diets, hospital diets, nursing diets, and the like, and a method of adding an alkali preparation (Patent Document 9) and a method of adding a protein-degrading enzyme (Patent Document 10) are known. ; Method of adding baked refined salt, sugar alcohol, starch derivative and organic acid (Patent Document 11); Method of adding calcium lactate, amino acid and carbohydrate (Patent Document 12); Method of adding alginate (Patent Document 12) Reference 13) and so on. In addition, due to problems such as excessive intake of ammonium peroxylate and calories, studies have been conducted on the use of soymilk or vegetable protein in place of cow's milk or animal meat, etc., but there are concerns about the fishy smell of soymilk or the unique smell of vegetable protein. On the other hand, the use of a specific yeast-derived yeast extract in order to enhance the flavor of spices and the like has been studied (Patent Document 14). Furthermore, with regard to the improvement of the taste and flavor of chocolate, it has been studied that the use of whey minerals enables the chocolate flavor to be strongly felt (Patent Document 16), and that it contains a large amount of glutamic acid and a large amount of nucleic acids. A food texture improving agent for chocolate products in which yeast extract is an active ingredient (Patent Document 17). With regard to dairy products, a masking agent for the light-deterioration odor of dairy products is proposed, which is characterized by containing a masking agent selected from the group consisting of 1,3-octanediol, 5-octene-1,3-diol, and dimethylmethoxyfuran The compound in the group consisting of ketones is used as an active ingredient (Patent Document 18); the production method of an acidic beverage containing milk is characterized by adding one or more of rutin, morin or quinone (Patent Document 18). Reference 19). Regarding thick milk taste, milk-like richness or sweetness, studies have been conducted to enhance thick milky taste or milk richness by using a specific yeast extract (Patent Documents 20 and 21); A powdered seasoning with a meat-like flavor prepared by heating a mixture of fat and yeast extract powder in a powdered state, and a cheese flavor and/or milk texture enhancer of yeast extract (Patent Document 22); fruit juice and yeast are extracted A seasoning for improving the flavor of dairy products, which is obtained by mixing and heating raw materials (Patent Document 23); a dairy-containing food product mainly composed of debittered dry yeast derived from the yeast recovered from the brewing process agent (Patent Document 24). Regarding foodstuffs using soybeans as a raw material, a soybean flavor imparting agent containing a lipase-treated product of soymilk has been studied (Patent Document 25). Regarding food products containing fish and livestock, studies have been conducted on the smell of sardines or chicken using extracts obtained by simultaneously subjecting distiller's grains to amylolytic enzyme treatment and proteolytic enzyme treatment and re-fermenting them with yeast (Patent Document 26); Odor suppression of shark meat by lactic acid bacteria-producing extract obtained by lactic acid fermentation of soymilk (Patent Document 27); Using the extract of aquatic animals as the main raw material and using Koji mold It processed, further added lactic acid bacteria and yeast, and fermented and aged moromi to obtain a previous extract or a fermented seasoning in which the odor of fish sauce was reduced (Patent Document 28). In addition, a deodorant composition for livestock meat containing, as an active ingredient, a water-soluble polysaccharide obtained from a fermented decomposition product obtained by fermenting a raw material containing soybeans by using Chlorokoji bacteria is studied (Patent Document 29); Method for producing beef or processed beef food in which arginine is added to meat pieces (Patent Document 30); using yeast extract containing 5% by mass or more of arginine with respect to the total solid content Suppression of animal meat odor and/or enhancement of target taste by composition composition (Patent Document 31); a method for processing food materials, characterized in that: by bringing baijiu koji sake or baijiu koji sweet cooking wine into contact with livestock meat or aquatic animals The production of aldehydes is inhibited, thereby reducing or inhibiting the odor of the above-mentioned livestock meat or aquatic animals (Patent Document 32); adding sweet cooking wines with aconitic acid content of 1 mg/L or more and 3000 mg/L or less into the boiled juice, the A method of imparting a rich taste by heating and conditioning livestock meat, thereby suppressing the odor of livestock meat (Patent Document 33); using lactones and/or lactone-containing substances to improve the grassy odor of beef or processed beef products. Production method (Patent Document 34); animal odor using a meat color improving agent for animal meat foods containing a specific amount of 5'-nucleotides, β-glucan, mannan, and a yeast extract of peptides as active ingredients shielding (Patent Document 35). Regarding fruit juice-containing beverages, a fruit juice feeling enhancer has been studied that has an inner branched cyclic structure part and an outer branched structure part and a glucan having a degree of polymerization of 50 or more as an active ingredient (Patent Document 36). However, no study has been conducted on the use of fermented seasonings using whey or soymilk as raw materials for these purposes. [Technical means to solve problems] The present inventors have conducted various studies on clarified lactic acid bacteria/yeast whey fermented liquid (refer to Patent Documents 1 and 2). Among them, it was found that there is a tendency that the texture of the ingredients tends to be maintained compared to the conventional frozen foods that are conditioned by using a fermented liquid of lactic acid bacteria and yeast to which whey or soymilk has been added. In addition, it was found that the fermented liquid of whey or soymilk using lactic acid bacteria and yeast can be used for softening meat, etc., shielding the fishy smell of soymilk, shielding the odor of vegetable protein, and enhancing the stimulation of spices. Further, it was found that the fermented liquid of whey or soymilk using lactic acid bacteria and yeast can be used to enhance the flavor of cocoa, inhibit the odor of light deterioration of milk or soymilk, enhance the milky feeling, enhance the cereal flavor of miso, mask the smell of fish, mask the smell of animal meat, The fruity feeling is improved, and the natto odor is masked. The present invention has been completed based on these findings. That is, the present invention provides the following. [1] A fermented seasoning composition, which uses the fermented product (lactic acid bacteria/yeast fermented product) produced by lactic acid bacteria and yeast of soymilk or whey as an active ingredient and is used to improve the texture of materials selected from foods, animal meat Or soften fish meat, mask the fishy smell of soy milk, mask the odor of vegetable protein, enhance the stimulation of spices, enhance the flavor of cocoa, suppress the odor of light deterioration of milk or soy milk, improve the milky feel, improve the grain flavor of miso, and mask the smell of fish meat , Any one of the group consisting of animal meat odor masking, fruitiness improvement, and natto odor masking. [2] The fermented seasoning composition according to 1, wherein the lactic acid bacteria are selected from the group consisting of Lactobacillus rhamnosus, Pediococcus acidlactici, Lactobacillus confusus, and Lactobacillus badensis (Lactobacillus malefermentans), Lactobacillus paracasei subsp. paracasei, Lactobacillus delbrueckii subsp. bulgaricus, Lactococcus lactis, Lactobacillus acidophilus, and Lactobacillus helveticus ( Lactobacillus helveticus) any of the group. [3] The fermented seasoning composition according to 1 or 2, wherein the yeast is selected from Saccharomyces cerevisiae, Saccharomyces sake, Saccharomyces beticus, Schizosaccharomyces pombe Any of the group of yeast (Schizosaccharomyces pombe). [4] The fermented seasoning composition according to any one of 1 to 3, wherein the lactic acid bacteria/yeast fermented product of soymilk is used as an active ingredient. [5] A food product comprising an effective amount of the lactic acid bacteria/yeast starter as defined in any one of 1 to 4. [6] A heat-conditioned refrigerated food or frozen food comprising an effective amount of the lactic acid bacteria/yeast starter as defined in any one of 1 to 4. [7] A food as described in 5, or refrigerated food or frozen food as described in 6, in the case of lactic acid bacteria/yeast fermented product using whey as a raw material, converted to lactic acid bacteria/yeast with a solid content of 10.7% In the case of lactic acid bacteria/yeast starter of fermented product, in the case of lactic acid bacteria/yeast starter product using soymilk as raw material, the lactic acid bacteria/yeast starter product with solid content of 6.5% contains 0.1-2.0% of lactic acid bacteria/yeast starter product . [8] A method for producing a refrigerated food or a frozen food, comprising the steps of: refrigerating or freezing a heat-conditioned food comprising an effective amount of the lactic acid bacteria/yeast ferment as defined in any one of 1 to 4 to obtain a obtain refrigerated or frozen food; or A kind of manufacture method of the food that comprises animal meat or fish meat, soybean milk, vegetable protein, spice, raw material derived from cocoa, milk, miso, tomato or fruit, or natto in raw material, it comprises the steps: adding any one of 1 to 4 Effective amount of lactic acid bacteria/yeast starter as defined in item. [9] A method for improving the texture of refrigerated food or frozen food, comprising the steps of: refrigerating a heat-conditioned food containing an effective amount of the lactic acid bacteria/yeast leaven as defined in any one of 1 to 4 refrigerated or frozen food; or A cereal selected from the group consisting of softening of animal meat or fish meat, masking of fishy odor of soybean milk, masking of odor of vegetable protein, or stimulation of spice, enhancing flavor of cocoa, suppressing light-deteriorating odor of milk or soybean milk, enhancing milky feeling, and of miso A method for any one of the group consisting of flavor enhancement, fish odor masking, animal meat odor masking, fruity feeling enhancement, and natto odor masking, comprising the steps of: adding the lactic acid bacteria defined in any one of 1 to 4 /Effective amount of yeast starter. [10] A method for producing a fermented seasoning composition using lactic acid bacteria/yeast fermented product as an active ingredient, comprising the steps of: further fermenting the lactic acid bacteria fermented product of soybean milk or whey by using yeast. [11] The fermented seasoning composition according to any one of 1 to 4, wherein the active ingredient comprises a component differentiated based on the molecular weight of the lactic acid bacteria/yeast fermented product, and the molecular weight of the lower limit of the component is 75 to 1,800, The molecular weight of the upper limit is 2,200 to 10,000. [12] A material selected from the group consisting of improving the texture of food, softening meat or fish, masking the fishy odor of soybean milk, masking the odor of vegetable protein, enhancing the stimulation of spices, enhancing the flavor of cocoa, and suppressing the odor of photo-deterioration of milk or soybean milk An agent for any one of the group consisting of enhancing milky texture, enhancing cereal flavor of miso, masking fish odor, masking animal meat odor, enhancing fruit texture, and masking natto odor, comprising lactic acid bacteria/yeast of soy milk or whey The fermented product has a molecular weight of 500 to 3,500. [1'] A use of soymilk or whey using a fermented product (lactobacillus/yeast fermented product) produced by lactic acid bacteria and yeast, which is used for improving the texture of food materials, softening animal meat or fish meat, and the fishy smell of soybean milk Masking, vegetable protein odor masking, spice stimulation enhancement, cocoa flavor enhancement, light deterioration odor suppression of milk or soy milk, milky texture enhancement, miso grain flavor enhancement, fish meat odor masking, animal meat odor masking, fruity flavor enhancement, and any purpose in the group consisting of natto odor masking. [2'] The use as described in 1', wherein the lactic acid bacteria are selected from Lactobacillus rhamnosus (Lactobacillus rhamnosus), Pediococcus acidlactici, Lactobacillus confusus, Lactobacillus malefermentans ) Lactobacillus paracasei subsp. paracasei, Lactobacillus delbrueckii subsp. bulgaricus, Lactococcus lactis, Lactobacillus acidophilus, and Lactobacillus helveticus any of the groups that form. [3'] The use as described in 1' or 2', wherein the yeast is selected from Saccharomyces cerevisiae, Saccharomyces sake, Saccharomyces beticus, Schizosaccharomyces pombe (Schizosaccharomyces pombe) any of the groups. [4'] The use according to any one of 1' to 3', wherein the lactic acid bacteria/yeast leaven of soymilk is used as an active ingredient. [5'] The use as described in any one of 1' to 4', wherein the active ingredient comprises components differentiated based on the molecular weight of lactic acid bacteria/yeast leaven, and the molecular weight of the lower limit of the components is 75-1,800, and the upper limit of the The molecular weight is 2,200 to 10,000. [6'] Use of a component with a molecular weight of 500-3,500 in lactic acid bacteria/yeast leaven of soybean milk or whey, which is used for improving the texture of food, softening meat or fish, masking the fishy smell of soybean milk, and plant Protein odor masking, spice stimulation enhancement, cocoa flavor enhancement, light deterioration odor suppression of milk or soymilk, milky texture enhancement, miso grain flavor enhancement, fish odor masking, animal meat odor masking, fruitiness enhancement, and natto odor Shade any of the objects in the group.

%及份除特別記載之情形以外,係以質量為基準。數值範圍「X~Y」除特別記載之情形以外,包含兩端之值X及Y。「A及/或B」除特別記載之情形以外,係指A、B中至少一者存在,亦包含A與B兩者均存在之情形。食品不僅為固體者,亦包含飲料及湯汁之類的液狀之經口攝取物。又,不僅為直接攝取之形態者(例如,經調理之各種食品、補充品、飲劑),亦包含食品添加物、醱酵調味料組合物、飲料濃縮物。進而,不僅為用於人類者,亦包含用於非人類動物(寵物、家畜等)者。又,食品除一般食品(包含所謂健康食品)以外,亦包含保健功能食品(功能性表示食品、營養功能食品、及特定保健用食品)。於稱為醱酵物時,其形態並無特別限定,可為液狀,亦可為固體狀。醱酵物只要可作為有效成分而發揮功能,則亦可為經各種處理者、例如將醱酵液進行殺菌、脫鹽、濃縮、成分調整、澄清化、及/或乾燥而成者。 本發明係關於一種以乳清或豆乳之利用乳酸菌及酵母所產生之醱酵物作為有效成分之用於食品之素材感改良等的醱酵調味料組合物。又,本發明係關於一種以利用酵母使乳清或豆乳之乳酸菌醱酵物進一步醱酵而獲得之乳酸菌/酵母醱酵物作為有效成分之用於食品之素材感改良等的醱酵調味料組合物、及其製造方法。再者,存在將利用乳酸菌及酵母所產生之醱酵物稱為乳酸菌/酵母醱酵物之情況。 [醱酵原料] 醱酵調味料組合物之原料係源自牛乳之純乳、乳清或豆乳,較佳為源自牛乳之乳清或豆乳。 可使用之乳清只要為可用作食品原料者,則無特別限定。乳清亦有稱為乳漿(whey)之情況,係自牛乳將作為乳蛋白質之主成分之酪蛋白與乳脂肪去除而成者,且係包含水溶性之蛋白質或乳糖、水溶性之維生素類或礦物質成分的水溶液狀之部分。乳清除了於自牛乳製作乳酪時作為副產物而產生以外,亦可以酸乳酪之上清液之形式獲得。乳清係以乾燥粉末之形式供給至市場,於本實施態樣中亦可較佳地使用此種乾燥品。關於供於醱酵之乳清之固形物成分濃度,只要利用乳酸菌所進行之醱酵較佳地進行,則無特別限定,但作為乳清粉末,於醱酵液中之起始濃度例如可設為5~40%,較佳為設為10~25%,更佳為設為12~18%。 可使用之豆乳只要為可用作食品原料者,則無特別限定。作為可較佳地使用之豆乳,可列舉:各種市售者、例如成分無調整豆乳(大豆固形物成分8%以上)、調整豆乳(大豆固形物成分6%以上)、豆乳飲料(大豆固形物成分4%以上)、豆乳之乾燥粉末。關於醱酵液之大豆固形物成分濃度,只要利用乳酸菌所進行之醱酵較佳地進行,則無特別限定,但醱酵液中之大豆固形物成分濃度例如可設為2~25%,較佳為設為5~20%。 醱酵所使用之乳清及豆乳於供於醱酵之前為了防止雜菌污染,亦可進行加熱殺菌。加熱殺菌之條件例如為80~90℃、30~60分鐘。 [乳酸菌醱酵] 於乳清或豆乳液之醱酵時,利用乳酸菌所進行之醱酵與利用酵母所進行之醱酵之順序可適當設定,於一較佳實施態樣中,首先進行利用乳酸菌所進行之醱酵。藉由依序進行利用厭氧性之乳酸菌所進行之醱酵、與如下述般一面通氣一面進行之利用酵母所進行之醱酵,可提供對各菌較佳之條件,例如即便因乳酸菌而pH值降低,亦因酵母耐受酸性,故而仍能生長等。又,藉此,醱酵步驟整體可以短時間結束。進而,可充分地運用源自酵母之香味成分。 所使用之乳酸菌只要為可用於食品製造者,則無特別限定,例如可使用選自由鼠李糖乳酸桿菌(Lactobacillus rhamnosus)、乳酸小球菌(Pediococcus acidlactici)、融合乳酸桿菌(Lactobacillus confusus)、壞醱酵乳酸桿菌(Lactobacillus malefermentans)、副乾酪乳酸桿菌(Lactobacillus paracasei subsp. paracasei)、保加利亞乳酸桿菌(Lactobacillus delbrueckii subsp. bulgaricus)、乳酸乳球菌(Lactococcus lactis)、嗜酸乳酸桿菌(Lactobacillus acidophilus)、及瑞士乳酸桿菌(Lactobacillus helveticus)等所組成之群中之任一種。較佳為選自由鼠李糖乳酸桿菌(Lactobacillus rhamnosus)、乳酸小球菌(Pediococcus acidlactici)、融合乳酸桿菌(Lactobacillus confusus)、壞醱酵乳酸桿菌(Lactobacillus malefermentans)、乳酸乳球菌(Lactococcus lactis)、及嗜酸乳酸桿菌(Lactobacillus acidophilus)所組成之群中之乳酸菌,更佳為鼠李糖乳酸桿菌(Lactobacillus rhamnosus)或乳酸小球菌(Pediococcus acidlactici)中之任一種。就生長性良好,醱酵風味亦優異之觀分而言,較佳為使用鼠李糖乳酸桿菌(Lactobacillus rhamnosus)。 於較佳之實施態樣中,作為乳酸菌,可使用鼠李糖乳酸桿菌AN1株或屬於與其相同之種且具有相同之科學性質之菌株、或者乳酸小球菌YU2株或屬於與其相同之種且具有相同之科學性質之菌株。 鼠李糖乳酸桿菌AN1株具有以下之科學性質。 細胞形態:桿狀 菌落:乳白色 特徵:進行異型乳酸醱酵。 乳酸小球菌YU2株具有以下之科學性質。 細胞形態:球狀 菌落:乳白色 特徵:四聯球菌,進行同型乳酸醱酵。 鼠李糖乳酸桿菌AN1株、及乳酸小球菌YU2株係分別依序以寄存編號NITE BP-02072、及NITE BP-02073,於2015年6月26日,由TableMark股份有限公司(住所:日本東京都中央區築地6-4-10),基於布達佩斯條約及日本專利法而寄存至獨立行政法人製品評價技術基盤機構(住所:日本千葉縣木更津市上總鐮足2-5-8 122號室)。 關於用於利用乳酸菌所進行之醱酵之條件,從業者可根據所使用之乳酸菌而適當地設計。培養液中除了乳清或豆乳以外,亦可添加各種者。作為碳源,例如可使用選自由甘蔗廢糖蜜、甜菜廢糖蜜、蔗糖、木材碎片蒸煮液、亞硫酸紙漿廢液、甘蔗萃取液、葡萄糖、乙酸、及乙醇所組成之群中之任一種。作為氮源,例如可使用選自由酵母萃取物、蛋白腖、玉米浸液(CSL)、酪蛋白等含氮有機物、以及脲、氨、硫酸銨、氯化銨、及磷酸銨等無機鹽所組成之群中之任一種。進而,亦可將磷酸成分、鉀成分、鎂成分添加至培養基中,亦可添加生物素、泛酸、硫胺素、肌醇、吡哆醇等維生素類;鋅、銅、鐵、錳等礦物質類。關於乳酸菌醱酵,雖然亦取決於所使用之菌株,但可以例如30~45℃、較佳為35~40℃進行例如16~48小時、較佳為20~36小時。考慮到乳酸菌為厭氧性,醱酵可一面進行靜置或輕度攪拌一面實施。又,醱酵亦可進行至pH值成為3.0~4.0、較佳為3.2~3.8之範圍。 [酵母醱酵] 於一較佳實施態樣中,可對以上述方式獲得之乳酸菌醱酵物,進行酵母醱酵作為下一步驟。 可用於酵母醱酵之酵母只要為可用於食品製造者,則無特別限定。例如可使用酒酵母、葡萄酒酵母、啤酒酵母等慣用之酵母。更具體而言,例如為選自由酵母菌(Saccharomyces)屬、裂殖酵母菌(Shizosaccharomyces)屬、畢赤氏酵母(Pichia)屬、念珠菌(Candida)屬、克魯維酵母(Kluyveromyces)屬、威洛蒲西絲(Williopsis)屬、德巴利氏酵母(Debaryomyces)屬、覆膜酵母菌(Galactomyces)屬、有孢圓酵母(Torulaspora)屬、紅酵母菌(Rhodotorula)屬、解脂亞羅(Yarrowia)屬、及接合酵母菌(Zygosaccharomyces)屬所組成之群中之任一種。關於酵母,就生長性良好之方面而言,較佳為用於麵包製造之麵包酵母、用於食料或飼料等之製造之圓酵母、用於啤酒製造之啤酒酵母,更佳為屬於酵母菌(Saccharomyces)之菌或屬於念珠菌(Candida)之菌。作為酵母菌屬之例,可列舉:釀酒酵母菌(Saccharomyces cerevisiae)、清酒酵母菌(Saccharomyces sake)、白葡萄酒酵母菌(Saccharomyces beticus)。再者,清酒酵母菌(Saccharomyces sake)與白葡萄酒酵母菌(Saccharomyces beticus)亦有被分類為釀酒酵母菌(Saccharomyces cerevisiae)之一種之情形。作為念珠菌屬之例,可列舉:熱帶念珠菌(Candida tropicalis)、解脂假絲酵母(Candida lypolitica)、高蛋白假絲酵母(Candida utilis)、清酒假絲酵母(Candida sake)。更佳為選自由釀酒酵母菌(Saccharomyces cerevisiae)、清酒酵母菌(Saccharomyces sake)、粟酒裂殖酵母菌(Schizosaccharomyces pombe)等所組成之群中之任一種。尤佳之酵母之一係亦視作釀酒酵母菌之一種之白葡萄酒酵母菌(Saccharomyces beticus),主要可列舉用於白葡萄酒之製造之產膜酵母。 於較佳之實施態樣中,作為酵母,可使用釀酒酵母菌FAHP-1株或屬於與其相同之種且具有相同之科學性質之菌株、或者釀酒酵母菌FAHP-2株或屬於與其相同之種且具有相同之科學性質之菌株。 釀酒酵母菌FAHP-1株具有以下之科學性質。 細胞形態:卵型 菌落:乳白色 特徵:形成具有醱酵能力之子囊孢子。 釀酒酵母菌FAHP-2株具有以下之科學性質。 細胞形態:卵型 菌落:乳白色 特徵:形成具有醱酵能力之子囊孢子。 釀酒酵母菌FAHP-1株、及釀酒酵母菌FAHP-2株係分別依序以寄存編號NITE BP-02074、及NITE BP-02075,於2015年6月26日藉由TableMark股份有限公司(住所:日本東京都中央區築地6-4-10),並基於布達佩斯條約及日本專利法而寄存至獨立行政法人製品評價技術基盤機構(住所:日本千葉縣木更津市上總鐮足2-5-8 122號室)。 關於用於利用酵母所進行之醱酵之條件,只要為業者,則可視所使用之酵母而適當地設計。於培養時,亦可對乳酸菌醱酵物進行滅菌。進而可向乳酸菌醱酵物添加各種者。作為碳源,例如可使用選自由甘蔗廢糖蜜、甜菜廢糖蜜、蔗糖、木材碎片蒸煮液、亞硫酸紙漿廢液、甘蔗萃取液、葡萄糖、乙酸、及乙醇所組成之群中之任一種。作為氮源,例如可使用選自由酵母萃取物、蛋白腖、玉米浸液(CSL)、酪蛋白等含氮有機物、以及脲、氨、硫酸銨、氯化銨、及磷酸銨等無機鹽所組成之群中之任一種。進而,亦可將磷酸成分、鉀成分、鎂成分添加至培養基中,亦可添加生物素、泛酸、硫胺素、肌醇、吡哆醇等維生素類;鋅、銅、鐵、錳等礦物質類。 酵母醱酵亦取決於所使用之菌株,可以例如20~40℃、較佳為25~35℃進行例如12~40小時、較佳為16~24小時。醱酵係於好氧條件下進行。視需要,可於酵母醱酵後一面進行充分之通氣及/或攪拌一面實施。關於通氣及/或攪拌,具體而言,可藉由每1分鐘將與醱酵液體積之約1/4量同量之無菌空氣吹入至醱酵槽底部,並以100~400 rpm進行攪拌而進行。再者,於酒類之製造步驟中,由於通常於厭氧條件下進行醱酵,故而酵母生成大量之乙醇。然而,於本實施態樣中,因於好氧條件下進行醱酵,故而將醱酵中之乙醇濃度保持為較低,酵母可生成較多之酯或脂肪酸、高級醇等各種香味成分。關於酵母醱酵過程及醱酵結束後之醱酵液中之醇濃度,較佳為未達1.0%。 關於酵母醱酵之終止點,只要為業者,則可適當地決定,例如可將隨著醱酵之進行而減少之布里克氏糖度(Brix)值之減少的停止、布里克氏糖度(Brix)值、及香味等設為基準。於將布里克氏糖度(Brix)值設為基準之情形時,以豆乳為原料時,可以5~9%、較佳為6~8%為基準,以乳清為原料時,可以9~13%、較佳為10~12%為基準。此處,所謂布里克氏糖度(Brix)值,係關於自液狀之醱酵物將源自乳清或豆乳之不溶性之固形物成分或乳酸菌菌體、及酵母之菌體去除而獲得者之值。於對醱酵物加鹽之情形時,並不包含該所添加之鹽分部分之值。 再者布里克氏糖度(Brix)值係由相當於20℃之蔗糖溶液之質量百分率之值而決定者,於利用糖量折光儀對僅包含蔗糖1 g作為溶質之水溶液100 g進行測定時,其讀數布里克氏糖度(Brix)值為1%。關於包含蔗糖以外之固體成分之溶液,布里克氏糖度(Brix)值係成為固體成分之濃度之標準。用以測定布里克氏糖度(Brix)值之機器係市售,只要為業者,則可適當地進行測定。 [其他步驟] 所獲得之乳酸菌/酵母醱酵物亦可為了提高保存穩定性而添加食鹽。食鹽之添加量並無特別限定,為了充分降低水分活性,可以相對於乳酸菌・酵母醱酵液,例如成為10%以上、較佳為15%以上、更佳為18%以上之方式進行添加。進而,所獲得之乳酸菌/酵母醱酵物因含有源自乳清或豆乳之不溶性之固形物成分或乳酸菌菌體、及酵母之菌體,故而於欲用於要求澄清性之商品之情形時,亦可進行澄清化處理,而將該等不溶性成分或菌體去除。關於澄清化之方法,可應用以相同之目的於食品之製造領域中使用之各種方法。例如可列舉:將矽藻土或波來鐵設為過濾助劑之壓濾機過濾、或精密過濾(MF:Micro filtration)。 [乳酸菌/酵母醱酵物(有效成分)] 以上述方式,自原料之乳清或豆乳獲得乳酸菌/酵母醱酵物。 關於乳酸菌/酵母醱酵物,作為其自經乳酸菌醱酵及酵母醱酵而獲得者將源自乳清或豆乳之不溶性之固形物成分或乳酸菌菌體、及酵母之菌體去除而獲得之液狀者,於為以乳清為原料時,布里克氏糖度(Brix)可為9~13%、較佳為10~12%,於為以豆乳為原料之情形時,布里克氏糖度(Brix)可為5~9%、較佳為6~8%。 乳酸菌/酵母醱酵物可為各種形態。例如,可視需要,將上述液狀者進行濃縮或乾燥等,而製成糊狀、固體狀、粉末狀、顆粒狀等。乳酸菌/酵母醱酵物亦有稱為W(double)醱酵調味料之情況,又,亦有簡稱為醱酵調味料等之情況。 若本發明者等人進一步進行研究,則可知如下情況:於乳清或豆乳之乳酸菌/酵母醱酵物中,分子量100~5,000之組分、更特定為分子量500~3,500之組分所包含之成分有助於目標之效果。因此,根據本發明,提供一種醱酵調味料組合物,其有效成分包含基於乳酸菌/酵母醱酵物之分子量而區分之組分,且組分之下限之分子量為75~1800、較佳為100~500,即便於下限為任一者之情形時,亦上限之分子量為2200~10000、較佳為3500~5000(例如分子量500~3500之組分、或分子量100~5000之組分)。又,提供一種食品之素材感改良劑,其包含乳清或豆乳之乳酸菌/酵母醱酵物之基於分子量而區分之組分,且組分之下限之分子量為75~1800、較佳為100~500,即便於下限為任一者之情形時,亦上限之分子量為2200~10000、較佳為3500~5000(例如分子量500~3500之組分、或分子量100~5000之組分)。 關於本發明及其實施態樣,所謂分子量,係指使用具有分子區分能力之透析管進行區分之分子量。關於乳清或豆乳之乳酸菌/酵母醱酵物之分子量500~3,500之組分(亦可為分子量100~5,000之組分),典型而言,係指至少使用區分分子量3500~5000之透析管(Spectra/Por,Funakoshi)及區分分子量100~500之透析管(Spectra/Por,Funakoshi),藉由通常之操作(若進一步特定,則為本說明書之實施例8項所記載之條件)進行區分而獲得之組分。再者,此處所列舉之透析管之區分分子量(Molecular Weight Cut Off:MWCO)係表示保持該大小之成分為90%以上。 作為本發明之實施態樣之一之利用酵母使以豆乳為原料之乳酸菌/酵母醱酵物、較佳為豆乳之乳酸菌醱酵物進一步醱酵而獲得之乳酸菌/酵母醱酵物係新穎者。 [醱酵調味料組合物] 所獲得之乳酸菌/酵母醱酵物可設為醱酵調味料組合物之有效成分。醱酵調味料組合物亦可為乳酸菌/酵母醱酵物其本身,有效成分只要可發揮目標之效果,則可調配其他成分。其他成分可為作為食品所容許之各種添加劑。關於該例,係抗氧化劑(antioxidant)、香料、調味料、甜味料、著色料、增黏穩定劑、顯色劑、漂白劑、防黴劑、原膠、苦味料等、酶、光澤劑、酸味料、乳化劑、強化劑、製造用劑、賦形劑、結合劑、繃緊化劑(等張化劑)、緩衝劑、溶解輔助劑、防腐劑、穩定劑、凝固劑等。醱酵調味料組合物中之有效成分之量例如為1~100%,可設為10~80%,亦可設為20~60%。 醱酵調味料組合物如下述般尤其適合用於食品之素材感改良、畜肉等之軟化、豆乳之腥味之遮蔽、植物蛋白質臭之遮蔽、或香辛料之刺激感之增強,因此,醱酵調味料組合物亦可另稱為「食品之素材感改良劑」、「畜肉等之軟化劑」、「豆乳之腥味之掩蔽劑」、「植物蛋白質臭之掩蔽劑」、「香辛料之刺激感之增強劑」、「可可風味提昇劑」、「牛乳或豆乳之光劣化臭之抑制劑」、「乳感提昇劑」、「味噌之穀物風味之提昇劑」、「魚肉臭掩蔽劑」、「畜肉臭掩蔽劑」、「果實感提昇劑」、及「納豆臭掩蔽劑」。即,本發明亦提供以下。 [1a]一種食品之素材感改良劑、畜肉等之軟化劑、豆乳之腥味之掩蔽劑、植物蛋白質臭之掩蔽劑、香辛料之刺激感之增強劑、可可風味提昇劑、牛乳或豆乳之光劣化臭之抑制劑、乳感提昇劑、味噌之穀物風味之提昇劑、魚肉臭掩蔽劑、畜肉臭掩蔽劑、果實感提昇劑、或納豆臭掩蔽劑,其以乳清或豆乳之乳酸菌/酵母醱酵物作為有效成分。 [2a]如1a記載之劑,其中乳酸菌為選自由鼠李糖乳酸桿菌(Lactobacillus rhamnosus)、乳酸小球菌(Pediococcus acidlactici)、融合乳酸桿菌(Lactobacillus confusus)、壞醱酵乳酸桿菌(Lactobacillus malefermentans)、副乾酪乳酸桿菌(Lactobacillus paracasei subsp. paracasei)、保加利亞乳酸桿菌(Lactobacillus delbrueckii subsp. bulgaricus)、乳酸乳球菌(Lactococcus lactis)、嗜酸乳酸桿菌(Lactobacillus acidophilus)、及瑞士乳酸桿菌(Lactobacillus helveticus)所組成之群中之任一種。 [3a]如1a或2a記載之劑,其中酵母為選自由釀酒酵母菌(Saccharomyces cerevisiae)、清酒酵母菌(Saccharomyces sake)、白葡萄酒酵母菌(Saccharomyces beticus)、粟酒裂殖酵母菌(Schizosaccharomyces pombe)所組成之群中之任一種。 [4a]如1a至3a中任一項記載之劑,其以利用醱酵使豆乳之乳酸菌醱酵物進一步進行醱酵而獲得之乳酸菌/酵母醱酵物作為有效成分。 [用途] <素材感改良> 所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物可用以改良食品之素材感。所謂素材感,係指可明顯地感覺到食品所包含之食材所特有之味道及/或食感(質地)。食品中包含各種食材,食材係於加熱調理時相互調和,出來味道而使作為食品整體之整體感增強。此時乃良好地感覺到調和之味道或柔順感,另一方面,若將經加熱調理之食品進行冷卻並於低溫下保存,則整體感或入味感會增加到並非意圖之程度。各素材所特有之味道或食感大大損失,而於食用時於口中變得難以判別各素材。本發明者等人進行研究,藉由添加乳酸菌/酵母醱酵物,而即便為加熱調理後進行冷卻而以冷藏或冷凍進行保存之食品,亦可較先前更能維持食材之素材感。因此,本發明之一態樣係含有所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之食品素材感之改良劑。 <畜肉等軟化> 所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物可用以使成為食材之畜肉或魚肉(包含以該等為原料之加工品。魚肉包含水產動物類之肉。亦有將該等總稱為「畜肉等」之情況)軟化。因此,本發明之一態樣係含有所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之畜肉等之軟化劑。 本發明之軟化劑亦可含於浸泡液等調味液中而注入(注射)至畜肉等中。或者,亦可藉由將畜肉等浸漬於含有本發明之軟化劑之浸漬液中而使用。或者,亦可含於例如漢堡肉餅等食品中。 藉由使用本發明之軟化劑,不僅使畜肉等柔軟,亦可提昇多汁感。此處所謂多汁感,係指於食用時咀嚼畜肉等時口中所感覺到之肉汁感。 <豆乳腥味遮蔽> 所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物亦可進而用以抑制豆乳(包含以其為原料之加工品)之腥味。因此,本發明之一態樣係含有乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之豆乳之腥味的掩蔽劑。本發明之腥味遮蔽之對象並不限於豆乳,亦可為以豆乳為原料之加工品、例如豆乳奶油。 藉由使用本發明之掩蔽劑,不僅遮蔽豆乳之腥味,亦可提昇甜味、柔順感、味道之厚重感。 <植物蛋白質臭遮蔽> 植物蛋白質、尤其是大豆蛋白質(包含以其為原料之加工品)具有獨特之氧化臭,但所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物可用以抑制此種植物蛋白質臭(亦有稱為「植物蛋白質臭」之情況)。因此,本發明之一態樣係含有乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之植物蛋白質臭之掩蔽劑。 本發明之乳酸菌/酵母醱酵物或醱酵調味料組合物因味道或風味非常淡,故而可一面運用素材之味道或風味一面遮蔽植物蛋白質臭。 藉由使用本發明之掩蔽劑,不僅遮蔽植物蛋白質之氧化味,亦可提昇柔軟感、多汁感、肉感。 <香辛料之刺激感之增強> 所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物可用以增強料理或調味液等所包含之香辛料(包含以其為原料之加工品)之刺激感。即,藉由將本發明之乳酸菌/酵母醱酵物或醱酵調味料組合物添加至含有香辛料之調味液或各種醬汁中,而可於食用時更強烈地感覺到香辛料之刺激,且使該刺激長期保持。因此,本發明之一態樣係含有乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之香辛料之刺激感(亦有稱為「香辛料感」之情況)之增強劑。 所謂香辛料,係指具有如下效果者,即藉由添加至食品中而使味道、香味、顏色、濃郁感、成熟感覺或刺激感(辣味)變化,而感覺到美味或使食慾增進。作為藉由本發明而增強刺激感之香辛料,並無限定,可列舉:黑胡椒、紅椒、青椒、胡椒、肉桂、肉豆蔻、丁香、眾香子、小茴香、陳皮、芫荽、大茴香、鼠尾草、百里香、月桂、茉沃刺、咖喱葉、芭樂、山椒、辣椒、芥末、生薑、蒜、辣椒粉、洋蔥、韭蔥、芝麻、五香粉、三味香辛料、咖喱粉、七味粉、五香辣椒粉等。 藉由使用本發明之增強劑,不僅增強香辛料之刺激感,亦可於成熟感覺、濃郁感、引起餘味之方面上提昇。 <可可風味提昇>  所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物可用以於含有源自可可之原料之食品中提昇可可之風味。即,藉由將本發明之乳酸菌/酵母醱酵物或醱酵調味料組合物添加至含有源自可可之原料之食品中,而可於食用時更強烈地感覺到可可之風味。因此,本發明之一態樣係含有乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之含有源自可可之原料之食品中的可可風味提昇劑。可可風味亦作為巧克力之風味(可可風味、濃啤酒風味、牛乳風味)之一而為人所知。 作為源自可可之原料,並無限定,可列舉:可可粒、可可塊、可可液、可可粉、可可餅、可可油、巧克力等。 <光劣化臭之抑制>  所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物亦可進而用以抑制牛乳(包含以其為原料之加工品)或豆乳(包含以其為原料之加工品)之光劣化臭。光劣化臭係指因由太陽光等引起之氧化而產生之氣體。關於光劣化臭抑制,可為由光引起之氧化劣化之抑制,又,亦可為因光劣化而產生之氣味之遮蔽。本發明之一態樣係含有乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之牛乳或豆乳之光劣化臭抑制劑。 <乳感之提昇>  所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物可用以提昇乳感。即,藉由將本發明之乳酸菌/酵母醱酵物或醱酵調味料組合物添加至使用有牛乳(包含以其為原料之加工品)之食品中,而可於食用時更強烈地感覺到乳感。因此,本發明之一態樣係含有乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之乳感提昇劑。所謂乳感之提昇,係指使牛乳所特有之良好風味提昇。 關於成為乳感提昇之對象之食品,並無限定,為牛乳或乳製品本身、及使用其作為原料之食品。 <味噌之穀物風味之提昇>  所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物可用以提昇味噌之穀物風味(大豆風味、米風味、或麥風味)。即,藉由將本發明之乳酸菌/酵母醱酵物或醱酵調味料組合物添加至含有味噌之食品中,而可於食用時更強烈地感覺到味噌之穀物風味。因此,本發明之一態樣係含有乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之味噌之穀物風味提昇劑。 作為味噌,並無限定,可列舉:白味噌、紅味噌、混合味噌、米味噌(使大豆與米進行醱酵、熟成而獲得者)、麥味噌(使大豆與大麥或裸麥進行醱酵、熟成而獲得者)、豆味噌(使大豆進行醱酵、熟成而獲得者)、調合味噌(將米味噌、麥味噌、豆味噌之各味噌進行混合而獲得者)等。較佳之例係白味噌、及調和味噌。關於通常經加熱殺菌之味噌或經冷凍乾燥之味噌、例如速溶味噌汁用之調味味噌,發現有味噌之風味變差之傾向,本發明之乳酸菌/酵母醱酵物或醱酵調味料組合物可較佳地用於提昇此種味噌之穀物風味。本發明之味噌之穀物風味提昇劑可應用於含有味噌之各種食品。 藉由使用本發明之味噌之穀物風味提昇劑,不僅提昇味噌之穀物風味,亦可提昇甜味及/或鮮味。 <魚肉臭遮蔽>  所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物可進而用以抑制魚肉臭。本發明之一態樣係含有乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之魚肉臭抑制劑。魚肉臭包含生魚之令人不適之臭味(腥味)、及經加熱之魚所具有之特有之氣味(魚臭)。 關於成為魚肉臭抑制之對象之食品,並不限於魚肉本身,亦可為使用魚肉作為原料之加工品、使用有該等之食品。 <畜肉臭遮蔽>  所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物可進而用以抑制畜肉臭。畜肉臭包含內臟臭、青草臭、及獸臭。所謂青草臭,係指自牧草養肥之牛獲得之牛肉所特有之草之腥味。本發明之一態樣係含有乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之畜肉臭抑制劑。 關於本發明之畜肉臭抑制劑之對象,並不限於畜肉,亦可為以其為原料之加工品、或以該等為原料之食品。再者,上述專利文獻30係提出利用精胺酸來減少畜肉之異味,但於上述專利文獻29中指出如下問題,即因精胺酸本身呈現苦味,故而亦向畜肉賦予苦味。根據本發明,認為即便使用有效量之本發明之有效成分,亦沒有上述問題。 <果實感之提昇>  所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物可用以提昇蕃茄或果實(包含以該等為原料之食品)之果實感。所謂果實感之提昇,係指提昇類似蕃茄(或果實)之良好之風味。本發明之一態樣係含有乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之源自果實之食品之果實感提昇劑。 關於成為對象之食品之尤佳例,係藉由長時間之燉煮加熱而調理者。藉由本發明,即便為此種燉煮加熱調理之食品,亦可提昇果實感。 藉由本發明,不僅提昇蕃茄或果實之果實感,亦可提昇酸味。通常存在若僅酸味增強,則食品之味道欠佳之傾向,但於利用本發明之情形時,蕃茄或果實係成為於提昇果實感之同時提昇酸味時,整體味道較佳者。 <納豆臭遮蔽>  所獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物亦可進而用以抑制納豆(包含以其為原料之加工品)之納豆臭。本發明之一態樣係含有乳酸菌/酵母醱酵物或醱酵調味料組合物作為有效成分之納豆之納豆臭抑制劑。 於用以抑制納豆臭之情形時,乳酸菌/酵母醱酵物或醱酵調味料組合物亦可直接添加至納豆中,又,亦可含於納豆之調料汁中,於食用時以與納豆混合之方式使用。 <評價方法及基準>  關於素材感是否得到改良、又素材感之改良之程度;畜肉等是否得到軟化、又該軟化之程度;豆乳之腥味是否得到遮蔽,又該遮蔽之程度;植物蛋白質臭是否得到遮蔽、又該遮蔽之程度;香辛料之刺激感是否得到增強、又該增強之程度;可可風味是否得到提昇、又該提昇之程度;牛乳或豆乳之光劣化臭是否得到抑制、又該抑制之程度;乳感是否得到提昇、又該提昇之程度;味噌之穀物風味是否得到提昇、又該提昇之程度;魚肉臭是否得到遮蔽、又該遮蔽之程度;畜肉臭是否得到遮蔽、又該遮蔽之程度;果實感是否得到提昇、又該提昇之程度;及納豆臭是否得到遮蔽、又該遮蔽之程度,只要為業者,則可計劃以適當之對照食品為基準之官能試驗而進行評價。更具體而言,例如準備不包含有效成分之食品等適當之對照,規定3~10等級左右之基準,基於基準(產業上有意義之基準,例如以將第7等級以上設為合格之方式進行規定),受過訓練之官能檢查員將成為對象之食品與對照進行比較,藉此可進行評價。 關於素材感之改良,於食品包含複數種食材之情形時,較佳為改良越發多之食材之素材感。本實施態樣之乳酸菌/酵母醱酵物或醱酵調味料組合物不僅改良蔬菜類之素材感,亦可改良肉類、水產動物類之素材感。關於畜肉等之軟化,亦可藉由對食品之硬度進行測定之方法而進行評價。於畜肉等之軟化中,較佳為一併提昇多汁感。於豆乳之腥味之遮蔽中,較佳為一併提昇甜味、柔順感、厚重感。於蛋白質臭之遮蔽中,較佳為一併提昇柔軟感、多汁感、肉感。於香辛料之刺激感之增強中,較佳為一併提昇濃郁感、成熟感覺、餘味。 作為用以更具體之評價之方法,可參照本說明書之實施例之項所記載之方法。 藉由本申請案而首次提供如下用途,即乳清或豆乳之乳酸菌/酵母醱酵物可用以改良食品之素材感。上述專利文獻1係關於乳清之乳酸菌・酵母醱酵液者,但並未提及用於在加熱調理後進行冷卻而進行冷藏或冷凍之食品。又,上述專利文獻2係記載有藉由將乳清之乳酸菌・酵母醱酵液添加至果實飲料中而增強果實本身之濃郁感與風味並賦予熟透風味,但並未提及用於在加熱調理後進行冷卻而進行冷藏或冷凍之食品。又,亦藉由本申請案而首次提供如下用途,即乳清或豆乳之乳酸菌/酵母醱酵物可用於畜肉等之軟化、豆乳腥味之遮蔽、植物蛋白質臭之遮蔽、香辛料之刺激感之增強、可可風味提昇、牛乳或豆乳之光劣化臭之抑制、乳感提昇、味噌之穀物風味之提昇、魚肉臭遮蔽、畜肉臭遮蔽、果實感提昇、及納豆臭遮蔽。 又,藉由本實施態樣而獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物可用以增強特定食品之鹹味。關於鹹味是否得到增強、又鹽味之增強之程度,只要為業者,則可計劃將適當之對照食品設為基準之官能試驗而進行評價。 [所應用之食品] 藉由本實施態樣而獲得之乳酸菌/酵母醱酵物或醱酵調味料組合物可應用於各種食品。關於所應用之食品之較佳例,係加熱調理後進行冷卻後而進行冷藏或冷凍之冷藏食品及冷凍食品、畜肉或魚肉(包含以該等為原料之加工品。魚肉包含水產動物類之肉。亦有將該等總稱為「畜肉等」之情況)、豆乳(包含以其為原料之加工品)、植物蛋白質(包含以其為原料之加工品)、以及香辛料(包含以其為原料之加工品)、含有源自可可之原料之食品、牛乳(包含以其為原料之加工品)、味噌(包含以其為原料之加工品)、蕃茄(包含以其為原料之加工品)、果實(包含以其為原料之加工品)、及納豆(包含以其為原料之加工品)。 作為冷藏食品及冷凍食品之例,例如可列舉:春捲、中華蓋飯(之配料)、餃子、燒賣、小籠包、肉包、八寶菜、回鍋肉、青椒肉絲、炒蔬菜、炒牛蒡絲、築前煮,除此以外,亦可列舉:炸牛肉薯餅、炸肉餅、炸蝦餅等油炸製品;漢堡肉餅等肉製品;澆汁烏龍麵、長崎雜燴、炒麵、那不勒斯式意麵、焗烤通心粉、千層麵等麵類;炒飯、菜肉燴飯、菜肉燜飯、什錦壽司等米飯製品;日式煎餅、章魚燒、比薩餅等小麥粉製品。作為畜肉等之例,並無特別限定,可列舉:牛肉、雞肉、豬肉、馬肉、羊肉、山羊肉、鱈魚、鮭魚等白身魚;金槍魚、鰹魚等赤身魚;蛤蜊、烏賊、章魚、蝦、蟹等。作為以畜肉或魚肉為原料之加工品之例,並無特別限定,可列舉各種軟化較佳者,具體而言,可列舉:香味烤、乾炸食品、附味油炸食品、天婦羅、西式油炸餅、炸豬排、炸雞排、炸牛排、炸蝦餅、烤肉用肉、牛排用肉、烤串用肉、燉煮用肉、火腿、臘肉等。作為以豆乳為原料之加工品之例,並無特別限定,可列舉各種腥味之遮蔽較佳者,具體而言,可列舉:豆乳奶油、咖啡飲料、紅茶飲料、可可飲料、豆乳酸乳酪、麵包類、分心類等。作為以植物蛋白質為原料之加工品之例,可列舉各種獨特之氧化味之遮蔽較佳者,具體而言,可列舉:漢堡肉餅、炸肉餅、肉丸子、西式牛肉、菜包肉、香腸等。更佳為以大豆蛋白質為原料之加工品。作為以香辛料為原料之加工品之例,並無特別限定,可列舉各種香辛料感之增強較佳者,具體而言,可列舉:日式煎餅醬汁、章魚燒醬汁、英國辣醬油、中濃醬汁、炸豬排醬汁、牛排醬汁、燒烤醬汁、烤肉之調味汁、草本鹽等。 作為含有源自可可之原料之食品之例,並無特別限定,可列舉:日式分心、烤分心、麵包、飲料、冷凍甜分、預加工食品、調味料等。作為成為光劣化臭之抑制之對象之食品的例,並無特別限定,可列舉:鮮奶、牛乳、成分調整牛乳、低脂肪牛乳、無脂肪牛乳、加工乳、奶油、黃油、乳脂肪、乳酪、濃縮乳清、冰淇淋類(冰淇淋、冰牛乳、乳酸冰淇淋等)、濃縮乳、脫脂濃縮乳、無糖煉乳、無糖脫脂煉乳、加糖煉乳、加糖脫脂煉乳、全粉乳、脫脂粉乳、乳油粉、乳清粉、蛋白質濃縮乳清粉、白脫乳粉、加糖粉乳、調整粉乳(育兒用奶粉等)、乳飲料、醱酵乳、及乳酸菌飲料。作為成為對象之食品之尤佳例,可列舉:豆乳、牛乳、及奶油。作為成為乳感提昇之對象之食品之其他例,並無特別限定,可列舉:酸乳酪、冰淇淋類、泡芙、奶油焦糖布丁、花蛋糕、白醬料、奶油醬、奶油炸牛肉薯餅、奶汁烤乾酪烙菜、及魚貝雞米飯等。作為成為味噌之穀物風味之提昇之對象的食品之例,並無特別限定,除味噌本身外,可列舉:速溶味噌汁、味噌醬。作為成為遮蔽魚肉臭之對象之食品之例,並無特別限定,可列舉:烤魚、魚肉加工品(魚肉香腸、魚醬、油魚糕、圓筒狀魚糕、魚肉山芋餅等)、魚加工品(乾貨、濕珍味、煮魚、用西京味增醃製之魚肉切片、魚肉罐頭等)、魚圓、水產動物類萃取物、鰹魚飯。作為成為遮蔽畜肉臭之對象之食品之例,並無特別限定,可列舉:食肉調製品(以牛肉、豬肉、其他食肉為原料者,且經加熱調理、或調味之製品或半加工品等)、具體而言,牛肉調製品(牛肉咖喱、牛肉乾、鹹味牛肉罐頭、烤牛肉、調味牛肉等)、豬肉調製品(調味豬肉、香腸、罐裝火腿、豬肉午餐肉等)、漢堡用之小餡餅、牛排用肉、烤肉用肉(牛舌、牛橫隔膜、肝臟等)、火腿、香腸、培根、調味豬排骨、牛肉蓋澆飯、豬肉蓋澆飯、雜碎鍋、漢堡肉餅、炸豬排、古老肉、豬肉天婦羅、餃子、美式豬肉燉豆。成為提昇果實感之對象之食品係蕃茄及果實(包含以其為原料之加工品),果實並無特別限定,包含柑橘類果實、例如檸檬、葡萄柚(白色果肉種類、紅色果肉種類)、酸橙、柳橙類(臍橙(Navel Orange)、晚崙西亞橙(Valencia Orange))、溫州蜜柑、焦柑(Tangor)、夏蜜柑、甘夏、八朔橘、日向夏橙、扁實檸檬、酸橘、柚子、臭橙、黃皮酸橙、伊予柑、蘆柑、金橘、三寶柑、白金柚、柚;核果類果實、例如杏子(別名杏)、櫻桃、梅、李子類(日本李子、洋李)、桃子類(桃子、油桃、黃桃);漿果類果實、例如葡萄(麝香葡萄、雷司令葡萄、特拉華葡萄、巨峰葡萄)、香蕉、黑莓、藍莓、木莓、醋栗(別名鵝莓)、石榴、蘋果、梨類(日本梨、中國梨、西洋梨)、木梨、幾維果、菠蘿、百香果、西印度群島櫻桃、荔枝、木通、蜜釋迦、鱷梨、無花果、橄欖、柿子、刺角瓜、番石榴、胡頹子、椰子、楊桃(別名Star fruit)、橘柚、番荔枝、榴蓮果、棗子、海棗、藍靛果、木瓜、火龍果、枇杷、桂圓、香肉果、甜瓜、榅桲、芒果、山竹、楊梅。又,果實包含果實型蔬菜、即草莓、西瓜、甜瓜。作為成為提昇果實感之對象之食品之較佳例,可列舉:蕃茄、草莓、蘋果、柳橙、蜜橘、藍莓、芒果、無花果、桃子。作為更佳之例,可列舉藉由長時間之燉煮加熱而調理之蕃茄醬汁、果實醬汁、果醬。作為成為納豆臭之遮蔽之對象之食品,係納豆及以其為原料之加工品,作為加工品之例,並無特別限定,包含嫩煎納豆、放有納豆之煎餅、放有納豆之漢堡肉餅、放有納豆之肉醬汁、放有納豆之壽司、放有納豆之湯、放有納豆之薄煎餅、納豆之天婦羅、納豆之多色麵條、放有納豆之麵汁。 乳酸菌/酵母醱酵物向食品之添加量雖然亦取決於食品之種類,但典型而言,相對於食品,於為以乳清為原料之乳酸菌/酵母醱酵物之情形時,換算為固形物成分10.7%之乳酸菌/酵母醱酵物,於為以豆乳為原料之乳酸菌/酵母醱酵物之情形時,換算為固形物成分6.5%之乳酸菌/酵母醱酵物而為0.1%以上,較佳為0.13%以上,更佳為0.17%以上,進而較佳為0.2%以上。其原因在於:若未達0.1%,則缺乏效果。為了遮蔽豆乳之腥味,尤佳為設為0.4%以上。關於添加量之上限,只要乳酸菌/酵母醱酵物之酸味或香味無損成為對象之食品之風味,則無限定。添加量之上限雖然亦取決於食品,但若超過2%,則會感覺到乳酸菌/酵母醱酵物之風味,因此較佳為2.0%以下,亦可設為1.8%以下,亦可設為1.5%以下。相對於容易感覺到雜味或澀味之味道較淡之食品、例如豆乳,亦可設為1.0%以下,又,就不會感覺到雜味、澀味,且獲得充分高之效果之觀點而言,亦可設為0.7%以下。 [其他] 本發明之實施態樣之一係一種冷藏食品或冷凍食品之製造方法,其包括如下步驟:將以上所說明之包含乳酸菌/酵母醱酵物之有效量的經加熱調理之食品進行冷藏或冷凍而獲得冷藏食品或冷凍食品。或者一種於原料中包含畜肉或魚肉、豆乳、植物蛋白質、香辛料、源自可可之原料、牛乳、味噌、蕃茄或果實、或者納豆之食品之製造方法,其包括如下步驟:添加乳酸菌/酵母醱酵物之有效量。關於食品之製造方法中之乳酸菌/酵母醱酵物之添加階段,可考慮作業性等而適當地設定。此處,所謂進行添加,包括注入及與之接觸。例如為了使畜肉等軟化,添加可為使軟化劑含有於浸泡液等調味液中並注入至畜肉等中之情況,亦可為將畜肉等浸漬於含有軟化劑之浸漬液中之情況,亦可為將軟化劑添加至原料或中間製品中並進行混合之情況。 作為本發明之另一實施態樣,可提供一種冷藏食品或冷凍食品之素材感之改良方法,其包括如下步驟:將以上所說明之包含乳酸菌/酵母醱酵物之有效量的經加熱調理之食品進行冷藏或冷凍而獲得冷藏食品或冷凍食品;或者一種用於畜肉或魚肉之軟化、豆乳之腥味遮蔽、植物蛋白質臭遮蔽、香辛料之刺激之增強、可可風味提昇、牛乳或豆乳之光劣化臭之抑制、乳感提昇、味噌之穀物風味之提昇、魚肉臭遮蔽、畜肉臭遮蔽、果實感提昇、或納豆臭遮蔽之方法,其包括如下步驟:添加乳酸菌/酵母醱酵物之有效量;一種以乳酸菌/酵母醱酵物作為有效成分之醱酵調味料組合物之製造方法,其包括如下步驟:利用酵母使乳清或豆乳之乳酸菌醱酵物進一步醱酵。 [實施例] 其次,藉由實施例及比較例而更詳細地說明本發明,但本發明之範圍並不限定於以下之實施例。 [實施例1]澄清加鹽乳酸菌、酵母乳清醱酵液之調製 將調配表示於以下。 [表1] (1)乳酸菌種子培養基組成 原料名 調配量 附註 脫脂粉乳 7.00 g 森永脫脂乳 酵母萃取物 0.07 g Difco:Bacto酵母萃取物 63.00 g 蒸餾水 (2)酵母種子培養基組成 原料名 調配量 附註 葡萄糖 0.70 g 和光純藥:特級 酵母萃取物 0.35 g Difco:Bacto酵母萃取物 大豆肽 0.35 g 不二製油:HI-NUTE DC6 68.60 g 蒸餾水 (3)乳酸醱酵液原料 原料名 調配量 附註 乳清粉末 300 g 四葉乳業 酵母萃取物 1.2 g Difco:Bacto酵母萃取物 1700 g 蒸餾水 (4)酵母醱酵液追加原料 原料名 調配量 附註 葡萄糖 30.0 g 和光純藥:特級 酵母萃取物 2.0 g Difco:Bacto酵母萃取物 68.0 g 蒸餾水 ・乳酸菌種子培養液 依據上述(1),於100 ml三角燒瓶中混合乳酸菌種子培養基之各原料並使該等溶解後,進行85℃、30分鐘之加熱殺菌,冷卻至36℃。將乳酸菌乳酸小球菌YU2株自MRS瓊脂培養基上以白金接種環接種至冷卻後之培養基中,以36℃進行24小時靜置培養而獲得乳酸菌種子培養液70 g。 ・酵母種子培養液 依據上述(2),於500 ml附帶擋板之三角燒瓶中混合酵母種子培養基之各原料並使該等溶解後,進行120℃、20分鐘之高壓釜滅菌,冷卻至30℃。將釀酒酵母菌FAHP-2株自YPD瓊脂培養基上以白金接種環接種至冷卻後之培養基中,以30℃進行24小時振盪培養(旋轉速度:200 rpm)而獲得酵母種子培養液70 g。 ・乳酸菌主醱酵 依據上述(3),於3升小型培養槽(ABLE公司製造)中混合各原料並使該等溶解後,進行85℃、30分鐘之加熱殺菌,冷卻至36℃。將上述之乳酸菌種子培養液70 g接種至冷卻後之液中後,一面以50 rpm進行微速攪拌一面以36℃進行24小時乳酸醱酵而獲得乳酸菌乳清醱酵液2070 g。 ・酵母主醱酵 依據上述(4),於100 ml三角燒瓶中混合各原料並使該等溶解後,進行120℃、20分鐘之高壓釜滅菌。冷卻至30℃後,添加至溫度已調整至30℃之3升小型培養槽中之乳酸菌乳清醱酵液中,於攪拌、混合後接種上述之酵母種子培養液70 g,於30℃下進行20小時通氣攪拌醱酵(攪拌速度:400 rpm,通氣量:1.0升/分鐘(0.5 vvm))而獲得乳酸菌/酵母乳清醱酵液2100 g。 再者,於酒類等之製造步驟中,由於通常於厭氧條件下進行醱酵,故而酵母生成大量之乙醇,但於本實施例中因藉由攪拌與通氣而於好氧條件下進行醱酵,故而醱酵中之乙醇濃度始終保持在1.0%以下,酵母主要生成酯或脂肪酸、高級醇等各種香味成分。 ・加鹽步驟 為了提高乳酸菌/酵母乳清醱酵液之保存性,將食鹽以成為20%之方式添加至最終製品中,一面進行攪拌一面使之溶解,藉此獲得加鹽乳酸菌/酵母乳清醱酵液。 ・過濾步驟 為了提高澄清性,利用精密過濾膜(MF)將加鹽乳酸菌/酵母乳清醱酵液進行過濾,而獲得澄清加鹽乳酸菌/酵母乳清醱酵液。MF係使用旭化成化學公司製造之Microza USP-143(標稱孔徑0.1 μm)。 ・殺菌步驟 於85℃之恆溫水槽中保持30分鐘而進行加熱殺菌,而獲得經殺菌之澄清加鹽乳酸菌/酵母乳清醱酵液(發明品1)2000 g。 ・分析步驟 對所獲得之發明品1進行各種分析。將結果示於下表。 [表2] 分析項目 分析值 分析方法 水分 70.0% 105℃、4 hr乾燥法 布里克氏糖度(Brix) 30.7% 折光式糖度計 pH值 4.0 pH值計 鹽分 20.0% 電位差滴定法 一般生菌數 10 cfu/g以下 標準瓊脂培養基法 大腸菌群 陰性 BGLB法 耐熱菌 10 cfu/g以下 標準瓊脂培養基法(100℃10分鐘) 黴・酵母 10 cfu/g以下 馬鈴薯葡萄糖瓊脂培養基法 [實施例2]澄清加鹽乳酸菌・酵母豆乳醱酵液之調製 將調配表示於以下。 [表3] (1)乳酸菌種子培養基組成 原料名 調配量 附註 豆乳 68.25 g 紀文:成分無調整豆乳 葡萄糖 1.40 g 和光純藥:特級 酵母萃取物 0.35 g Difco:Bacto酵母萃取物 (2)酵母種子培養基組成 原料名 調配量 附註 葡萄糖 0.70 g 和光純藥:特級 酵母萃取物 0.35 g Difco:Bacto酵母萃取物 大豆肽 0.35 g 不二製油:HI-NUTE DC6 68.60 g 蒸餾水 (3)乳酸醱酵液原料 原料名 調配量 附註 豆乳 1950 g 紀文:成分無調整豆乳 葡萄糖 40 g 和光純藥:特級 酵母萃取物 10 g Difco:Bacto酵母萃取物 (4)酵母醱酵液追加原料 原料名 調配量 附註 葡萄糖 30.0 g 和光純藥:特級 酵母萃取物 2.0 g Difco:Bacto酵母萃取物 68.0 g 蒸餾水 ・乳酸菌種子培養液 依據上述(1),於100 ml三角燒瓶中混合乳酸菌種子培養基之各原料並使該等溶解後,進行85℃、30分鐘之加熱殺菌,冷卻至36℃。將乳酸菌鼠李糖乳酸桿菌AN1株自MRS瓊脂培養基上以白金接種環接種至冷卻後之培養基中,以36℃進行24小時靜置培養而獲得乳酸菌種子培養液70 g。 ・酵母種子培養液 依據上述(2),於500 ml附帶擋板之三角燒瓶中混合酵母種子培養基之各原料並使該等溶解後,進行120℃、20分鐘之高壓釜滅菌,冷卻至30℃。將釀酒酵母菌FAHP-1株自YPD瓊脂培養基上以白金接種環接種至冷卻後之培養基中,以30℃進行24小時振盪培養(旋轉速度:200 rpm)而獲得酵母種子培養液70 g。 ・乳酸菌主醱酵 依據上述(3),於3升小型培養槽(ABLE公司製造)中混合各原料並使該等溶解後,進行85℃、30分鐘之加熱殺菌,冷卻至36℃。將上述之乳酸菌種子培養液70 g接種至冷卻後之液中後,一面以50 rpm進行微速攪拌一面以36℃進行24小時乳酸醱酵而獲得乳酸菌豆乳醱酵液2070 g。 ・酵母主醱酵 依據上述(4),於100 ml三角燒瓶中混合各原料並使該等溶解後,進行120℃、20分鐘之高壓釜滅菌。冷卻至30℃後,添加至溫度已調整至30℃之3升小型培養槽中之乳酸菌豆乳醱酵液中,於攪拌、混合後接種上述之酵母種子培養液70 g,於30℃下進行20小時通氣攪拌醱酵(攪拌速度:400 rpm,通氣量:1.0升/分鐘(0.5 vvm))而獲得乳酸菌・酵母豆乳醱酵液2100 g。 ・加鹽步驟 為了提高乳酸菌・酵母豆乳醱酵液之保存性,將食鹽以成為20%之方式添加至最終製品中,一面進行攪拌一面使之溶解,藉此獲得加鹽乳酸菌・酵母豆乳醱酵液。 ・過濾步驟 為了提高澄清性,利用精密過濾膜(MF)將加鹽乳酸菌・酵母豆乳醱酵液進行過濾,而獲得澄清加鹽乳酸菌・酵母豆乳醱酵液。MF係使用旭化成化學公司製造之Microza USP-143(標稱孔徑0.1 μm)。 ・殺菌步驟 於85℃之恆溫水槽中保持30分鐘而進行加熱殺菌,而獲得經殺菌之澄清加鹽乳酸菌・酵母豆乳醱酵液(發明品2)2000 g。 ・分析步驟 對所獲得之發明品2進行各種分析。將結果示於下表。 [表4] 分析項目 分析值 分析方法 水分 78.20% 105℃、4 hr乾燥法 布里克氏糖度(Brix) 27.40% 折光式糖度計 pH值 3.5 pH值計 鹽分 20.90% 電位差滴定法 一般生菌數 10 cfu/g以下 標準瓊脂培養基法 大腸菌群 陰性 BGLB法 耐熱菌 10 cfu/g以下 標準瓊脂培養基法(100℃10分鐘) 黴・酵母 10 cfu/g以下 馬鈴薯葡萄糖瓊脂培養基法 [實施例3]冷凍春捲試作 依據下表及下述之程序,確認經殺菌之澄清加鹽乳酸菌/酵母乳清醱酵液(發明品1)向冷凍春捲之添加效果。 [表5] Control Blank 試驗1 試驗2 試驗3 試驗4 試驗5 試驗6 乳清醱酵液添加比率 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% 食材 豬里脊肉 12.00% 12.00% 12.00% 12.00% 12.00% 12.00% 12.00% 12.00% 捲心菜 22.00% 22.00% 22.00% 22.00% 22.00% 22.00% 22.00% 22.00% 20.00% 20.00% 20.00% 20.00% 20.00% 20.00% 20.00% 20.00% 4.00% 4.00% 4.00% 4.00% 4.00% 4.00% 4.00% 4.00% 乾燥香菇 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 粒狀植物性蛋白 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 豬油 3.00% 3.00% 3.00% 3.00% 3.00% 3.00% 3.00% 3.00% 生薑泥 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 調味料 上等白糖 0.67% 0.67% 0.67% 0.67% 0.67% 0.67% 0.67% 0.67% 麩胺酸鈉 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 蠔油 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 醬油 0.40% 0.40% 0.40% 0.40% 0.40% 0.40% 0.40% 0.40% 食鹽 0.40% 0.40% 0.39% 0.38% 0.36% 0.30% 0.20% 0.00% 日本酒 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 豬肉精 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 芝麻油 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 澱粉 2.67% 2.67% 2.67% 2.67% 2.67% 2.67% 2.67% 2.67% 發明品1 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% 25.2% 25.2% 25.2% 25.1% 25.0% 24.8% 24.4% 23.6% Total 100% 100% 100% 100% 100% 100% 100% 100% 冷凍處理 ・程序 (1)預先將調配表5之原料中之芝麻油以外之調味料原料進行混合。 (2)將1大匙油添加至已預熱之煎鍋中並輕度地煎炒肉類。 (3)添加其他食材並進行煎炒。 (4)將食材過火後添加調味料混合液並進行加熱。 (5)發黏後關火,添加芝麻油並取至墊上。 (6)用市售之春捲皮包裹食材30 g。 (7)於170℃之油中油炸2分鐘。 (8)於室溫下冷卻後,於-25℃之冷凍庫中冷凍保管2週。 僅將對照(Control)品另外供於評價試驗,於將空白(Blank)或試驗1~6之冷凍春捲進行加熱解凍之時點同時進行調製,將Blank或試驗1~6之冷凍春捲以未冷凍保管之狀態供於評價試驗。 ・評價方法 冷凍保管2週後,利用微波爐將各春捲試作品進行解凍,受過充分訓練之14名官能檢查員係針對素材感及風味之良好度,將Control(非冷凍品)設為10分,將Blank(無添加冷凍品)設為5分,以10等級評價進行官能評價,並求出各官能檢查員之評價分數之平均值。再者,於對個位之有效數字進行四捨五入而成為7分以上之情形時,係賦予了先前品所未有之充分之素材感或風味者,而規定為合格(於以下之實施例中相同)。 ・評價基準(素材感) 1分:完全無素材感。 2分:遠遠差於Blank。 3分:差於Blank。 4分:稍差於Blank。 5分:與Blank(無添加冷凍品)相同。 6分:較Blank稍微提昇。 7分:較Blank提昇,1~2種食材有素材感。 8分:較Blank提昇,3種以上之食材有素材感。 9分:素材感充分。稍差於Control。 10分:與Control(非冷凍品)相同,或者有較其優異之素材感。 ・評價基準(風味之良好度) 1分:風味非常差。 2分:遠遠差於Blank。 3分:差於Blank。 4分:稍差於Blank。 5分:與Blank(無添加冷凍品)相同。 6分:較Blank稍微提昇。 7分:較Blank提昇。 8分:較Blank提昇,有類似春捲之風味。 9分:類似春捲之風味充分。稍差於Control。 10分:與Control(非冷凍品)相同,或者有較其優異之風味。 ・評價結果 將結果示於圖1、2。春捲食材之素材感係隨著發明品1之添加量增加而提昇,有效之添加量為0.1%以上。春捲整體之風味之良好度亦同樣地隨著發明品1之添加量增加而提昇,但若添加量為2%,則可稍微感覺到作為乳清原液之酸味或香味,而有喪失類似春捲食感之傾向。 因此,於本實施例之春捲中,發明品1之實用上之有效添加量可謂0.1%~2.0%。 [實施例4]冷凍春捲試作(發明品2) 依據下表、及下述之程序,確認經殺菌之澄清加鹽乳酸菌・酵母豆乳醱酵液(發明品2)向冷凍春捲之添加效果。 [表6] Control Blank 試驗1 試驗2 試驗3 試驗4 試驗5 試驗6 豆乳醱酵液添加比率 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% 食材 豬里脊肉 12.00% 12.00% 12.00% 12.00% 12.00% 12.00% 12.00% 12.00% 捲心菜 22.00% 22.00% 22.00% 22.00% 22.00% 22.00% 22.00% 22.00% 20.00% 20.00% 20.00% 20.00% 20.00% 20.00% 20.00% 20.00% 4.00% 4.00% 4.00% 4.00% 4.00% 4.00% 4.00% 4.00% 乾燥香菇 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 粒狀植物性蛋白 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 豬油 3.00% 3.00% 3.00% 3.00% 3.00% 3.00% 3.00% 3.00% 生薑泥 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 調味料 上等白糖 0.67% 0.67% 0.67% 0.67% 0.67% 0.67% 0.67% 0.67% 麩胺酸鈉 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 蠔油 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 醬油 0.40% 0.40% 0.40% 0.40% 0.40% 0.40% 0.40% 0.40% 食鹽 0.40% 0.40% 0.39% 0.38% 0.36% 0.30% 0.20% 0.00% 日本酒 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 豬肉精 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 芝麻油 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 澱粉 2.67% 2.67% 2.67% 2.67% 2.67% 2.67% 2.67% 2.67% 發明品2 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% 25.2% 25.2% 25.2% 25.1% 25.0% 24.8% 24.4% 23.6% Total 100% 100% 100% 100% 100% 100% 100% 100% 冷凍處理 ・程序 以與實施例3相同之程序進行調理,而調製冷凍食品。 ・評價方法、評價基準 以與實施例3相同之方式進行。 ・評價結果 將結果示於圖3及4。春捲食材之素材感係隨著發明品2之添加量增加而提昇,有效之添加量係與發明品1同樣地為0.1%以上。春捲整體之風味之良好度亦同樣地隨著發明品2之添加量增加而提昇,但若添加量為2%,則可稍微感覺作為豆乳醱酵液之酸味或香味,而有喪失類似春捲食感之傾向。 因此,於本實施例中,發明品2之實用上之有效添加量可謂0.1%~2.0%。 [實施例5]中華蓋飯之配料試作(發明品1) 依據下表、及下述之程序,確認經殺菌之澄清加鹽乳酸菌/酵母乳清醱酵液(發明品1)向冷凍中華蓋飯之配料之添加效果。 [表7] Control Blank 試驗1 試驗2 試驗3 試驗4 試驗5 試驗6 發明品添加比率 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% 食材 3.0% 3.0% 3.0% 3.0% 3.0% 3.0% 3.0% 3.0% 烏賊 4.0% 4.0% 4.0% 4.0% 4.0% 4.0% 4.0% 4.0% 胡蘿蔔 3.0% 3.0% 3.0% 3.0% 3.0% 3.0% 3.0% 3.0% 白菜 15.0% 15.0% 15.0% 15.0% 15.0% 15.0% 15.0% 15.0% 4.0% 4.0% 4.0% 4.0% 4.0% 4.0% 4.0% 4.0% 木耳 1.5% 1.5% 1.5% 1.5% 1.5% 1.5% 1.5% 1.5% 豆莢 0.5% 0.5% 0.5% 0.5% 0.5% 0.5% 0.5% 0.5% 豬肉 3.5% 3.5% 3.5% 3.5% 3.5% 3.5% 3.5% 3.5% 調味汁 蔥末 0.31% 0.31% 0.31% 0.31% 0.31% 0.31% 0.31% 0.31% 生薑泥 0.17% 0.17% 0.17% 0.17% 0.17% 0.17% 0.17% 0.17% 日本酒 2.17% 2.17% 2.17% 2.17% 2.17% 2.17% 2.17% 2.17% 食鹽 0.40% 0.40% 0.39% 0.38% 0.36% 0.30% 0.20% 0.00% 砂糖 1.13% 1.13% 1.13% 1.13% 1.13% 1.13% 1.13% 1.13% 麩胺酸鈉 0.31% 0.31% 0.31% 0.31% 0.31% 0.31% 0.31% 0.31% 胡椒 0.06% 0.06% 0.06% 0.06% 0.06% 0.06% 0.06% 0.06% 雞肉粉湯 1.21% 1.21% 1.21% 1.21% 1.21% 1.21% 1.21% 1.21% 海鮮精 2.06% 2.06% 2.06% 2.06% 2.06% 2.06% 2.06% 2.06% 中華高湯粉 0.52% 0.52% 0.52% 0.52% 0.52% 0.52% 0.52% 0.52% 醬油 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 澱粉 2.60% 2.60% 2.60% 2.60% 2.60% 2.60% 2.60% 2.60% 發明品1 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% 52.94% 52.94% 52.90% 52.86% 52.78% 52.54% 52.14% 51.34% 芝麻油 0.62% 0.62% 0.62% 0.62% 0.62% 0.62% 0.62% 0.62% 合計 100.0% 100.0% 100.0% 100.0% 100.0% 100.0% 100.0% 100.0% 冷凍處理 ・程序 (1)預先將表中之調味汁原料之一部分(自日本酒直至發明品1)進行混合。 (2)將食材一個一個地進行焯水並取至墊上。 (3)向鍋中投入蔥末與生薑泥。 (4)投入(1)並進行加熱。 (5)發黏後關火。 (6)投入芝麻油並進行混合。 (7)投入經焯水之食材並進行混合。 (8)於室溫下冷卻後,於-25℃之冷凍庫中冷凍保管2週。 僅將Control品另外供於評價試驗,於將Blank或試驗1~6之中華蓋飯之配料進行加熱解凍之時點同時進行調製,將Blank或試驗1~6之中華蓋飯之配料以未冷凍保管之狀態供於評價試驗。 ・評價方法 冷凍保管2週後,利用微波爐將各中華蓋飯之配料試作品進行解凍,受過充分訓練之14名官能檢查員係針對素材感・食材感、風味之良好度,將Control(非冷凍品)設為10分,將Blank(無添加冷凍品)設為5分,以10等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價基準(素材感) 1分:完全沒有素材感。 2分:遠遠差於Blank。 3分:差於Blank。 4分:稍差於Blank。 5分:與Blank(無添加冷凍品)相同。 6分:較Blank稍微提昇。 7分:較Blank提昇,1~3種食材有素材感。 8分:較Blank提昇,4種以上之食材有素材感。 9分:素材感充分。稍差於Control。 10分:與Control(非冷凍品)相同,或者有較其優異之素材感。 ・評價基準(風味之良好度) 1分:風味非常差。 2分:遠遠差於Blank。 3分:差於Blank。 4分:稍差於Blank。 5分:與Blank(無添加冷凍品)相同。 6分:較Blank稍微提昇。 7分:較Blank提昇。 8分:較Blank提昇,有類似中華蓋飯之配料之風味。 9分:類似中華蓋飯之配料之風味充分。稍差於Control。 10分:與Control(非冷凍品)相同,或者有較其優異之風味。 ・評價結果 將結果示於圖5、6。 [實施例6]中華蓋飯之配料試作(發明品2) 於實施例5之表中,將發明品1變更為發明品2,除此以外,以相同之調配並依據下述之程序,確認澄清加鹽乳酸菌・酵母豆乳醱酵液(發明品2)向冷凍春捲之添加效果。 ・程序 利用與實施例5相同之程序進行調理而調製冷凍食品。 ・評價方法、評價基準 以與實施例5相同之方式實施。 ・評價結果 將結果示於圖7、8及下表。又,官能檢查員之試食評語係如下表所示。 [表8] Control Blank 試驗1 試驗2 試驗3 試驗4 試驗5 試驗6 添加比率 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% 評語 ・普通之味道 ・稍微感到有所欠缺 ・難以判明食材之風味 ・作為冷凍品為普通之味道 ・與Blank相比並無較大差別 ・感覺到極少之白菜之風味 ・強烈感覺到食材之風味 ・容易判明白菜、木耳之味道 ・雖不是非冷凍品,但感覺到強烈之食材感 ・稍強烈地感覺到鹹味 ・感覺到接近非冷凍品之食材感、風味 ・強烈感覺到鹹味 ・感覺到接近非冷凍品之食材感、風味 ・非常強烈地感覺到鹹味 ・醱酵液之風味較強,稍有不調和之感覺 ・鹹味較強 根據食品,可見促進鹹味之效果。 [實施例7]利用不同菌株之醱酵液之調製 使用其他乳酸菌或酵母,除此以外,依據實施例1或2而調製醱酵液。 [表9]    實施例1 實施例2 實施例7 實施例8 實施例9 實施例10 原料 乳清 豆乳 豆乳 乳清 豆乳 豆乳 乳酸菌 乳酸小球菌YU2株 鼠李糖乳酸桿菌AN1株 乳酸小球菌 嗜酸乳酸桿菌 融合乳酸桿菌 壞醱酵乳酸桿菌 酵母 釀酒酵母菌FAHP-2 釀酒酵母菌(白葡萄酒)FAHP-1 釀酒酵母菌 粟酒裂殖酵母菌 釀酒酵母菌 釀酒酵母菌(清酒) 關於調製之評語 3升小型培養槽試驗 培養條件係如實施例1中記載所述 3升小型培養槽試驗 培養條件係如實施例1中記載所述 燒瓶培養試驗。基本條件係與實施例1、2相同 燒瓶培養試驗。基本條件係與實施例1、2相同 燒瓶培養試驗。基本條件係與實施例1、2相同 燒瓶培養試驗。基本條件係與實施例1、2相同 關於醱酵液之評語 酯香與果實香較強 甜香味較強 雖乳酪之風味較強之方面不同,但獲得與實施例1同樣之醱酵液。 雖乙酸乙酯臭較強,但獲得與實施例1同樣之醱酵液。 雖如煉乳之甜香味較強之方面不同,但獲得與實施例1同樣之醱酵液。 雖酸乳酪風味與果實香較強,但獲得與實施例1同樣之醱酵液。 實施例7~10中所獲得之醱酵液均為可期待目標之效果者。 [實施例8]含有成分之區分試驗 依據下述之程序,調製澄清加鹽乳酸菌/酵母乳清醱酵液(發明品1)所含有之成分之區分液。 ・程序 (1)藉由減壓濃縮而將澄清加鹽乳酸菌/酵母乳清醱酵液濃縮至二倍。 (2)向區分分子量20000之透析管(Spectra/Por,Funakoshi)填充(1),一面進行攪拌一面於蒸餾水中進行透析(4℃、24小時、蒸餾水1L×3次交換)。 (3)將透析管內之樣品設為區分液1。 (4)將蒸餾水側以成為與透析前等量之方式進行減壓濃縮。 (5)向區分分子量3500~5000之透析管(Spectra/Por,Funakoshi)填充(4),一面進行攪拌一面於蒸餾水中進行透析(4℃、24小時、蒸餾水1L×3次交換)。 (6)將透析管內之樣品設為區分液2。 (7)將蒸餾水側以成為與透析前等量之方式進行減壓濃縮。 (8)向區分分子量100~500之透析管(Spectra/Por,Funakoshi)填充(7),一面進行攪拌一面於蒸餾水中進行透析(4℃、24小時、蒸餾水1L×3次交換)。 (9)將透析管內之樣品設為區分液3。 (10)將蒸餾水側以成為與透析前等量之方式進行減壓濃縮而設為區分液4。 ・區分液詳細內容 將各區分液所含有之成分之分子量示於下表。 [表10] 區分液 分子量 區分1 分子量>20000 區分2 (3500~)5000<分子量<20000 區分3 (100~)500<分子量<3500(~5000) 區分4 分子量<100(~500) ・各區分液之評價結果 藉由與實施例3相同之方法,確認澄清加鹽乳酸菌/酵母乳清醱酵液之區分液向冷凍春捲之添加效果。 [表11] 素材感評價 Control 發明品1 2倍濃縮物 8 區分1 分子量>20000組分 5 區分2 (3500~)5000<分子量<20000組分 6 區分3 (100~)500<分子量<3500(~5000)組分 8 區分4 分子量<100(~500)組分 6 將結果示於上表。春捲食材之素材感係隨著區分分子量變小而強烈地感覺到,於區分液3添加區中,感覺到與發明品1同等之素材感。因此,於本實施例之條件中,認為發明品1之有效成分係分子量(100~)500~3500(~5000)之成分。 [實施例9] 香烤嫩雞塊試作試驗(發明品2)  依據下表、及以下之程序,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向香烤嫩雞塊之添加效果。 [表12] Blank 試驗1 發明品2 0.00% 0.33% 碳酸氫鈉(製劑) 0.37% 0.00% 雞胸脯肉 74.07% 74.10% 食鹽 0.81% 0.82% 上等白糖 3.19% 3.19% 液糖 0.74% 0.74% 醬油 2.22% 2.22% 清酒 2.00% 2.00% 雞精 0.22% 0.22% 植物油脂 0.22% 0.22% 加工澱粉 2.07% 2.07% 14.07% 14.08% Total 100.00% 100.00% ・程序 (1)將調配表所記載之調味液原料(雞胸脯肉以外之原料)進行混合。 (2)切割雞胸脯肉,與(1)中所獲得之調味液進行混合而進行浸漬。 (3)將所獲得之雞胸脯肉於烘箱中進行加熱。 (4)將加熱過之雞胸脯肉進行急速冷凍。 ・評價方法 冷凍保管3天後,利用微波爐進行解凍後,冷卻至室溫,受過充分訓練之10名官能檢查員係針對食感之柔和度與多汁感,將Blank(無添加品)設為5分,以10等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價基準(各評價項目通用) 1分:較Blank非常地差。 2分:遠遠差於Blank。 3分:差於Blank。 4分:稍差於Blank。 5分:與Blank(無添加品)相同。 6分:較Blank提昇一點。 7分:較Blank稍微提昇。 8分:較Blank提昇。 9分:較Blank充分地提昇。 10分:較Blank極大地提昇。 ・評價結果 將結果示於圖9。發明品2添加品係如下評價:較碳酸氫鈉製劑添加品柔軟且多汁。 ・關於上述之程序中所獲得之雞胸脯肉,於冷凍保管3天後,利用微波爐進行解凍後,冷卻至室溫,利用Texture analyzer(Stable Micro Systems公司製造),使用山電楔型柱塞No.49,於速度1.0 mm/sec及變形率100%之條件下進行破碎試驗。將其結果示於圖10。發明品2添加品較碳酸氫鈉製劑添加品,以較弱之力破碎。 [實施例10]無調整豆乳添加試驗(發明品2) 依據下表、及以下之程序,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向無調整豆乳之添加效果。再者,於根據事前試驗之結果而得知可獲得較高之效果之添加量範圍0.4%~1%內進行試驗。 [表13] Blank 試驗1 試驗2 試驗3 試驗4 試驗5 試驗6 試驗7 發明品2 0.00% 0.40% 0.50% 0.60% 0.70% 0.80% 0.90% 1.00% 無調整豆乳(紀文) 99.80% 99.48% 99.40% 99.32% 99.24% 99.16% 99.08% 99.00% 食鹽 0.20% 0.12% 0.10% 0.08% 0.06% 0.04% 0.02% 0.00% Total 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% ・程序 向無調整豆乳(紀文)添加發明品2(0、0.4、0.5、0.6、0.7、0.8、0.9、1%),並利用食鹽補充鹽分。 ・評價方法 受過充分訓練之10名官能檢查員係針對甜味、柔順感、味道之厚重感、腥味之遮蔽,將Blank(無添加品)設為5分,以10等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價基準(各評價項目通用) 1分:非常弱。 2分:遠遠弱於Blank。 3分:弱於Blank。 4分:稍弱於Blank。 5分:與Blank(無添加品)相同 。 6分:較Blank強一點點。 7分:較Blank稍強。 8分:較Blank強。 9分:較Blank充分地強。 10分:較Blank非常地強。 ・評價結果 將結果示於圖11。發明品2添加品係如下評價:與無添加品相比,甜味、柔順感、味道之厚重感增加,且遮蔽了豆乳之腥味。全部項目成比例地上升直至添加率0.7%,其後上升變緩慢。又,若添加率成為1.0%,則出現雜味或澀味。 [實施例11]漢堡肉餅添加試驗(發明品2) 依據下表、及以下之程序,於將肉末之40%變更為植物蛋白質(大豆蛋白質)之漢堡肉餅中確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)之植物蛋白質臭遮蔽效果。再者,將作為比較對象之先前用於植物蛋白質臭遮蔽之純米料理酒以成為相同製造成本之方式進行添加。 [表14] Blank 試驗1 試驗2 調配A 牛肉豬肉混合肉末(7:3) 40.94% 40.94% 40.94% 食鹽 0.45% 0.45% 0.45% 炒洋蔥※2 13.65% 13.65% 13.65% 生麵包粉 6.82% 6.82% 6.82% 全蛋 4.78% 4.78% 4.78% 牛乳 4.78% 4.78% 4.78% 番茄汁 1.02% 1.02% 1.02% 肉豆蔻粉末 0.10% 0.10% 0.10% 黑胡椒(粉末) 0.10% 0.10% 0.10% 大蒜(粉末) 0.07% 0.07% 0.07% 植物蛋白質+調味液 粒狀植物性蛋白質※1 6.82% 6.82% 6.82% 20.15% 17.76% 18.88% 純米料理酒 2.44% 發明品2 1.59% 食鹽 0.32% 0.26% 0.00% Total 100.00% 100.00% 100.00% ※1 不二製油公司製造「New Fujinic 41SH」 ※2 炒洋蔥:利用煎鍋將無鹽黃油30 g、沙拉油30 g進行加熱,煎炒洋蔥500 g與月桂樹皮2片,進行冷卻後使用。 ・程序 (1)將植物蛋白質浸漬於調味液中30分鐘。 (2)混合調配A之材料,進行攪合直至發黏。 (3)將浸漬過之植物蛋白質添加至調配A中,進而進行攪拌。 (4)於冷藏庫中醱酵30分鐘。 (5)分割為各自120 g,進行成型後,於烘箱中以230℃加熱10分鐘。 (6)急速冷凍後,進行真空包裝而進行冷凍保管。 (7)試食時用熱水進行加熱。 ・評價方法 受過充分訓練之10名官能檢查員係針對植物蛋白質臭遮蔽、柔軟感、多汁感、肉感,將Blank(無添加品)設為5分,以10等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價基準(各評價項目通用) 1分:較Blank非常地差。 2分:遠遠差於Blank。 3分:差於Blank。 4分:稍差於Blank。 5分:與Blank(無添加品)相同。 6分:較Blank提昇一點點。 7分:較Blank稍微提昇。 8分:較Blank提昇。 9分:較Blank充分地提昇。 10分:較Blank極大地提昇。 ・評價結果 將結果示於圖12。發明品2添加品與無添加品或純米料理酒相比,全部之評價項目得到了提昇。尤其是植物蛋白質臭遮蔽效果較高。又,料理酒被賦予了酒獨特之風味。然而,豆乳醱酵液添加品係於風味本身未改變之情況下僅被賦予了各種效果。 [實施例12]日式煎餅醬汁添加試驗(發明品2) 依據下表及以下之程序,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向日式煎餅醬汁之添加效果。 [表15] Control 試驗1 日式煎餅醬汁 發明品2 0.00% 0.20% 糖類(液糖) 35.00% 35.00% 糖類(砂糖) 4.60% 4.60% 蔬菜/果實(蕃茄) 8.00% 8.00% 蘋果醬 2.80% 2.80% 蘋果濃縮果汁 2.40% 2.40% 蔬菜/果實(洋蔥) 0.20% 0.20% 蔬菜/果實(蒜) 0.20% 0.20% 蛋白水解物 8.86% 8.86% 釀造醋 5.09% 5.09% 食鹽 1.00% 1.00% 香辛料※1 0.20% 0.20% 增黏劑 2.50% 2.50% 色素 1.00% 1.00% 化學調味料 0.46% 0.46% 原料水 27.69% 27.49% Total 100.00% 100.00% ※1 使用將黑胡椒、辣椒、肉桂、肉豆蔻、丁香、鼠尾草、百里香、月桂、陳皮混合而成者。 ・程序 (1)向調配有表中所記載之原料之日式煎餅醬汁添加豆乳醱酵液(發明品2)。 ・評價方法 受過充分訓練之10名官能檢查員係針對香辛料感、酸味、成熟感覺、濃郁感,將Blank(無添加品)設為5分,以10等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價基準(各評價項目共通) 1分:較Blank非常地差。 2分:遠遠差於Blank。 3分:差於Blank。 4分:稍差於Blank。 5分:與Blank(無添加品)相同。 6分:較Blank提昇一點點。 7分:較Blank稍微提昇。 8分:較Blank提昇。 9分:較Blank充分地提昇。 10分:較Blank極大地提昇。 ・評價結果 將結果示於圖13。發明品2添加品與無添加品相比,香辛料感、成熟感覺、濃郁感得到了提昇。尤其是香辛料感(刺激感)之提昇明顯。又,亦有引起餘味之效果。 [實施例13]熱巧克力試作試驗(發明品2) 依據下表、及以下之程序,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向熱巧克力之添加效果。 [表16] Blank 試驗1 可可漿 10.84% 10.84% 砂糖 10.84% 10.84% 無調整豆乳 77.47% 77.47% 發明品2 0.00% 0.85% 食鹽水 0.85% 0.00% 合計 100.00% 100.00% ・程序 (1)用熱水使可可漿溶化。 (2)將無調整豆乳加熱至50℃,將其約半量注入至已溶化之可可漿中並進行混合。 (3)亦添加剩餘之豆乳、及砂糖並進行混合。 (4)混合食鹽水或發明品2。 ・評價方法 試作後,針對設為10℃、20℃、40℃之樣品,受過充分訓練之4名官能檢查員係針對可可風味,將Blank(無添加品)設為5分,以10等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價基準 1分:非常弱。 2分:遠遠弱於Blank。 3分:弱於Blank。 4分:稍微弱於Blank。 5分:與Blank(無添加品)相同。 6分:較Blank強一點點。 7分:較Blank稍強。 8分:較Blank強。 9分:較Blank充分地強。 10分:較Blank非常地強。 ・評價結果 將結果示於圖14。發明品2添加品(試驗1)係如下評價:較Blank,可可風味得到了提昇。又,溫度越高,可可感提昇效果越大。 [實施例14]奶油巧克力添加試驗(發明品2) 依據下表、及以下之程序,確認經殺菌之澄清乳酸菌・豆乳醱酵液(發明品2,但係未加鹽者)向奶油巧克力之添加效果。 [表17] Blank 試驗1 板狀奶油巧克力 99.0% 99.0% 1.0% 0.0% 發明品2(無食鹽) 0.0% 1.0% 合計 100.0% 100.0% ・程序 (1)用熱水使奶油巧克力溶化。 (2)混合水或發明品2。 ・評價方法 試作後,針對進行冷卻而成為固體之樣品及40℃至液體樣品,受過充分訓練之6名官能檢查員係針對可可風味,將Blank(無添加品)設為5分,以10等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價基準 1分:非常弱。 2分:遠遠弱於Blank。 3分:弱於Blank。 4分:稍弱於Blank。 5分:與Blank(無添加品)相同。 6分:較Blank強一點點。 7分:較Blank稍強。 8分:較Blank強。 9分:較Blank充分地強。 10分:較Blank非常地強。 ・評價結果 將結果示於圖15。發明品2添加品(試驗1)係如下評價:較Blank,可可風味得到了提昇。又,液體者之可可風味提昇效果較大。 [實施例15]光照射牛乳、豆乳添加試驗(發明品2) 依據下表、及以下之程序,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向光照射牛乳、光照射豆乳之添加效果。 [表18] Blank 試驗1 牛乳 99.5% 99.5% 發明品2 0.0% 0.5% 食鹽水 0.5% 0.0% 合計 100.0% 100.0% Blank 試驗1 無調整豆乳 99.0% 99.0% 發明品2 0.0% 1.0% 食鹽水 1.0% 0.0% 合計 100.0% 100.0% ・程序 (1)於牛乳或無調整豆乳中混合食鹽水或發明品2。 (2)藉由附帶照明之培養箱FLI-2010T型(EYELA東京理化器械股份有限公司)進行光照射(5℃、26000勒克司、5天) ・評價方法 針對5天後之樣品,受過充分訓練之2名官能檢查員係針對光劣化臭抑制效果,將進行光照射之Blank設為1分,將未照射光之樣品設為10分,以10等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價基準 1分:光劣化臭抑制效果非常地弱(光劣化臭較未照射光之Blank非常地強,但與進行光照射之Blank同等)。 2分:光劣化臭抑制效果很弱(光劣化臭較未照射光之Blank非常地強,但微弱於進行光照射之Blank) 3分:光劣化臭抑制效果較弱(光劣化臭較未照射光之Blank非常地強,但稍弱於進行光照射之Blank)。 4分:光劣化臭抑制效果稍弱(光劣化臭較未照射光之Blank非常地強,但弱於進行光照射之Blank)。 5分:光劣化臭抑制效果微弱(光劣化臭較未照射光之Blank非常地強,但遠遠弱於進行光照射之Blank)。 6分:光劣化臭抑制效果微強(光劣化臭遠遠強於未進行光照射之Blank)。 7分:光劣化臭抑制效果稍強(光劣化臭強於未進行光照射之Blank)。 8分:光劣化臭抑制效果較強(光劣化臭稍強於未進行光照射之Blank)。 9分:光劣化臭抑制效果很強(光劣化臭較未進行光照射之Blank強一點點)。 10分:光劣化臭抑制效果非常地強(光劣化臭與未進行光照射之Blank(完全)相同)。 ・評價結果 將結果示於圖16及17。雖牛乳、豆乳均會由於光照射而產生劣化臭,但發明品2添加品(試驗1)係如下評價:較Blank,該光劣化臭得到了抑制。 [實施例16]奶油試作試驗(發明品2) 如下表所示,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向奶油之添加效果。 [表19] Blank 試驗1 油層 植物性油脂 29.7% 29.7% 卵磷脂 1.5% 1.5% 水層 牛乳 58.4% 58.4% 脂肪酸甘油酯 0.2% 0.2% 酪蛋白鈉 0.1% 0.1% 偏磷酸鈉 0.1% 0.1% 發明品2 0.0% 0.1% 乳脂原料 生奶油 10.0% 9.9% 合計 100.0% 100.0% ・程序 (1)油層 對油進行加熱,於使之溶解時添加卵磷脂、乳化劑並進行混合。 (2)水層 對水分進行加熱,添加材料並進行混合。 (3)將水層與油層進行混合而進行預乳化,其後,進行均質化處理並進行冷卻。 ・評價方法 受過充分訓練之2名官能檢查員係針對所獲得之樣品之乳風味進行官能評價。 ・評價結果 發明品2添加品係如下評價:較Blank,乳風味得到了提昇。 [實施例17]奶油炸牛肉薯餅試作試驗(發明品2) 如下表所示,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向奶油炸牛肉薯餅中配料之添加效果。 [表20] 原材料名 Blank 試驗1 小麥粉 5.00% 5.00% 加工澱粉 3.00% 3.00% 食鹽 0.20% 0.20% 蟹片 5.00% 5.00% 上等白糖 1.20% 1.20% 清湯粉末 0.50% 0.50% 9.10% 9.00% 牛乳 30.00% 30.00% 生奶油 5.00% 5.00% 人造黃油 1.50% 1.50% 醱酵黃油 0.10% 0.10% 洋蔥 30.00% 30.00% 沙拉油 2.20% 2.20% 加工助劑 4.20% 4.20% 海鮮精 2.00% 2.00% 發明品2 0.00% 0.10% 蛋黃 1.00% 1.00% 合計 100.00% 100.00% ・程序 (1)煎炒洋蔥後,混合表中所記載之原材料並進行加熱。 (2)將(1)中所獲得之配料進行冷卻,使之凝固後,進行成型並進行包衣, (3)將(2)進行油炸(180℃3分鐘)而獲得奶油炸牛肉薯餅。 ・評價方法 受過充分訓練之2名官能檢查員係針對所獲得之樣品之乳風味進行官能評價。 ・評價結果 發明品2添加品(試驗1)係如下評價:較Blank,乳風味得到了提昇。 [實施例18]向速溶味噌汁之添加試驗(發明品2) 依據下表、及以下之程序,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向速溶味噌汁之添加效果。作為速溶味噌汁用之調味味噌,使用有調和味噌(永谷園製「Asage」原封味噌型,使用大豆味噌)、赤味噌(永谷園製「Hiruge」原封味噌型,使用大豆味噌)、白味噌(永谷園製「Yu-Ge」原封味噌型,使用大豆味噌)。 [表21] 原材料名 Blank 試驗1 調味味噌 9.25% 9.25% 食鹽水 0.20% 0.00% 發明品2 0.00% 0.20% 熱水 90.56% 90.56% 合計 100.00% 100.00% ・程序 (1)將調味味噌、與食鹽水及發明品2進行混合。 (2)注入熱水並進行混合。 ・評價方法 試作後,針對冷卻至室溫之樣品,受過充分訓練之4名官能檢查員係針對穀物風味(大豆風味)、甜味、鮮味之強度,將Blank(無添加品)設為5分,以10等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價基準 1分:非常地弱。 2分:遠遠地弱於Blank。 3分:弱於Blank。 4分:稍弱於Blank。 5分:與Blank(無添加品)相同。 6分:較Blank強一點點。 7分:較Blank稍強。 8分:較Blank強。 9分:較Blank充分地強。 10分:較Blank非常地強。 ・評價結果 將結果示於圖18。發明品2添加品(試驗1)係如下評價:較Blank,穀物風味(大豆風味)、甜味、鮮味得到了提昇。其效果於白味噌與調和味噌之速溶味噌汁中尤大。又,除所記載之原封味噌型外,即便為冷凍乾燥型之速溶味噌汁,亦確認到同樣之效果。 [實施例19]向鮭魚松之添加試驗(發明品2) 依據下表、及以下之程序,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向鮭魚松之添加效果。 [表22] [%] 原材料名 Blank 試驗1 鮭魚松 10.00 10.00 發明品2 - 0.20 食鹽 0.04 - 熱水 89.96 89.80 合計 100.00 100.00 ・程序 (1)將表中所記載之量之熱水、食鹽水及發明品2進行混合。 (2)將(1)中混合而成之液與鮭魚松進行混合。 ・評價方法 針對所獲得之樣品,受過充分訓練之2名官能檢查員係針對魚肉之腥味與魚臭,將Blank設為+++(3分),以3等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。再者,將生魚之令人不適之臭味稱為「腥味」,將經加熱之魚所具有之特有氣味稱為「魚臭」。 ・評價結果 將結果示於表中。發明品2添加品係如下評價:較Blank,魚肉之腥味或魚臭得到了抑制。 [表23] 樣品名 腥味 魚臭 評價 Blank +++ +++ 有特有之魚臭、腥味 試驗1 + + 整體魚臭減弱,未感覺到腥味 弱 + <---> +++ 強 [實施例20]向內臟鍋湯之添加試驗(發明品2) 依據下表、及以下之程序,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向內臟鍋湯之添加效果。 [表24] 調味液 [%] 原材料名 Blank 試驗1 淡味醬油 5.70 5.70 風味高湯(海帶)* 0.30 0.30 炊味廚房鰹魚L* 0.30 0.30 L-麩胺酸鈉 0.10 0.10 牛肉精* 2.00 2.00 發明品2 - 0.20 食鹽 0.04 - 91.56 91.40 合計 100.00 100.00 *富士食品工業公司製造 [表25] 內臟鍋湯 原材料名 調配率[%] 水煮豬雜 20 上述調味液 80 合計 100 ・程序 (1)將表中所記載之原材料進行混合而製成調味液。 (2)將調味液與水煮豬雜以表之調配率填充至袋中,以90℃進行10分鐘加熱殺菌,將其進行水冷後進行冷凍,而製成內臟鍋湯。 (3)將內臟鍋湯解凍後,連同包裝一起進行加熱而製成官能評價用樣品。 ・評價方法 針對藉由上述程序而獲得之樣品,受過充分訓練之2名官能檢查員係針對內臟氣味,將Blank設為+++(3分),以3等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價結果 將結果示於表中。發明品2添加品係如下評價:較Blank,內臟之氣味得到了抑制。 [表26] 樣品名 內臟氣味 評價 Blank +++ 有內臟所特有之獸臭 試驗1 + 內臟之氣體得到了抑制 弱 + <---> +++ 強 [實施例21]向牛肉蓋澆飯食材之添加試驗(發明品2) 依據下表、及以下之程序,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向牛肉蓋澆飯(食材)之添加效果。 [表27] 牛肉蓋澆飯之原料調味液 [%] 原材料名 Blank 試驗1 濃味醬油 12.00 12.00 淡味醬油 12.00 12.00 上等白糖 6.50 6.50 釀造甜料酒 2.50 2.50 白葡萄酒 2.00 2.00 柳橙100%果汁 1.50 1.50 乾鰹魚削片萃取物P* 1.50 1.50 穀物醋(酸度4,2%) 1.00 1.00 濃味海帶高湯* 0.60 0.60 加工澱粉 0.60 0.60 生薑提取物 0.10 0.10 50.00 50.00 食鹽 0.30 0.15 發明品2 - 0.74 調製水 9.40 8.81 合計 100.00 100.00 *富士食品工業公司製造 [表28] 牛肉蓋澆飯之原料 原材料名 調配率[%] 牛肉(澳洲產) 40.6 洋蔥 32.4 左記牛肉蓋澆飯之原料調味液 27.0 合計 100.0 ・程序 (1)將表中所記載之原材料進行混合,用熱水加熱至85℃後,進行冷卻而製成調味液。 (2)將牛肉與洋蔥煮沸後,進行冷卻。 (3)將牛肉、洋蔥、調味液以表中所記載之調配率放入包裝中,以90℃加熱10分鐘。將其水冷後,進行冷凍而製成牛肉蓋澆飯之原料。 (4)將(3)之牛肉蓋澆飯之原料解凍後,連同包裝一起進行加熱而製成官能評價用樣品。 ・評價方法 針對藉由上述程序而獲得之樣品,受過充分訓練之2名官能檢查員係針對牛肉之青草臭,將Blank設為+++(3分),以3等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價結果 將結果示於表中。發明品2添加品係如下評價:較Blank,牛肉之青草臭得到了抑制。 [表29] 樣品名 青草臭 評價 Blank +++ 有肉之青草(草)臭 試驗1 + 整體臭味消失 弱 + <---> +++ 強 [實施例22]向調味豬肉之添加試驗(發明品2) 依據下表、及以下之程序,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向調味豬肉之添加效果。 [表30] [%] 原材料名 Blank 試驗1 調味豬肉(5 mm)末 100 100 發明品2 - 0.2 合計 100 100.2 ・程序 (1)利用碎肉機,將調味豬肉以半解凍之狀態製成5 mm之肉末。 (2)於成為肉末之調味豬肉中混合表中所記載之其他材料並放入包裝中,以90℃加熱10分鐘。將其冷卻後,製成官能評價用樣品。 ・評價方法 針對藉由上述程序而獲得之樣品,受過充分訓練之2名官能檢查員係針對調味豬肉之畜肉臭,將Blank設為+++(3分),以3等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價結果 將結果示於表中。發明品2添加品係如下評價:較Blank,豬肉之獸臭得到了抑制。 [表31] 樣品名 畜肉臭 評價 Blank +++ 有豬肉之獸臭 試驗1 + 整體豬肉之獸臭消失 弱 + <---> +++ 強 [實施例23]向蕃茄醬汁之添加試驗(發明品2) 依據下表、及以下之程序,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向蕃茄醬汁之添加效果。 [表32] (蕃茄醬汁調配) 原材料 調配率[%] 洋蔥(切成末) 25.00 去皮整番茄 19.50 蕃茄泥 13.00 蕃茄糊 5.20 白葡萄酒 3.00 橄欖油 3.00 蒜泥 1.00 砂糖 1.00 三仙膠 0.10 白胡椒 0.10 大蒜粉末 0.05 月桂樹皮粉末 0.03 食鹽 0.70 28.32 合計 100.00 [表33] [%] 原材料名 Blank 試驗1 蕃茄醬汁 99.00 99.00 食鹽 0.10 0.06 發明品2 - 0.20 0.90 0.74 合計 100.00 100.00 ・程序 (1)預先將洋蔥切成末,且三仙膠係與砂糖進行混合並溶化,香辛料係與調味料進行混合並溶化。 (2)向鍋中添加橄欖油,煎炒洋蔥,添加蕃茄並輕度過火。 (3)向(2)添加(1)與其他原料,並添加香辛料與白葡萄酒。 (4)將(3)放入袋中,以90℃加熱40分鐘。 (5)將(4)冷卻後,緩緩地加熱而製成官能評價用樣品。 ・評價方法 針對藉由上述程序而獲得之樣品,受過充分訓練之2名官能檢查員係針對蕃茄醬汁之果實感與酸味,將Blank設為3分,以5等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價結果 將結果示於表中。發明品2添加品係如下評價:較Blank,蕃茄醬汁之果實感或酸味得到了提昇。 [表34] 樣品名 果實感 酸味 評價 Blank 3 3 有爽快之酸味之醬汁 試驗1 5 4 蕃茄之果實感上升之醬汁 [實施例24]向納豆之添加試驗(發明品2) 依據下表、及以下之程序,確認經殺菌之澄清加鹽乳酸菌・豆乳醱酵液(發明品2)向納豆之添加效果。 [表35] 納豆之調料汁 [%] 原材料名 Blank 試驗1 淡味醬油 58.8 58.8 淡口胺基酸液 25.4 25.4 葡萄糖果糖液糖 9.8 9.8 L-麩胺酸鈉 3.4 3.4 乾鰹魚削片萃取物粉末M-6* 0.5 0.5 IG-Na2 0.1 0.1 發明品2 - 0.2 食鹽 0.2 0.16 調製水 1.8 1.64 合計 100.00 100.00 *富士食品工業公司製造 ・程序 (1)將表中所記載之原材料進行混合而製成納豆之調料汁。 (2)相對於納豆(Okame Natto(Takanofoods製造))50 g,添加(1)之調料汁3.7 g。用筷子攪拌30次而製成官能評價用樣品。 ・評價方法 針對藉由上述程序而獲得之樣品,受過充分訓練之2名官能檢查員係針對納豆臭,將Blank設為+++(3分),以3等級評價進行官能評價,並求出各官能檢查員之評價分之平均值。 ・評價結果 將結果示於表中。發明品2添加品係如下評價:較Blank,納豆臭得到了抑制。 [表36] 樣品名 納豆臭 評價 Blank +++ 有納豆特有之氣味 試驗1 + 難以感覺到納豆臭明顯減弱 弱 + <---> +++ 強 % and part are based on quality unless otherwise stated. The numerical range "X to Y" includes the values X and Y at both ends unless otherwise specified. "A and/or B" means that at least one of A and B exists, and also includes the situation where both A and B exist, unless otherwise specified. The food is not only solid, but also includes liquid oral ingestion such as drinks and soups. Moreover, not only those in the form of direct ingestion (for example, prepared various foods, supplements, and beverages), but also food additives, fermented seasoning compositions, and beverage concentrates. Furthermore, not only those used for humans but also those used for non-human animals (pets, livestock, etc.) are included. In addition, the foods include general foods (including so-called health foods) and health functional foods (functional foods, nutritional functional foods, and foods for specific health uses). When it is called a fermented product, its form is not particularly limited, and it may be a liquid state or a solid state. As long as the fermented product can function as an active ingredient, it may be obtained by various treatments such as sterilization, desalination, concentration, component adjustment, clarification, and/or drying of the fermented fermented liquid. The present invention relates to a fermented seasoning composition for improving the texture of foods, etc., using as an active ingredient a fermented product produced by lactic acid bacteria and yeast of whey or soymilk. Furthermore, the present invention relates to a fermented seasoning composition for improving the texture of food materials, using lactic acid bacteria/yeast fermented product obtained by further fermenting whey or soymilk lactic acid bacteria fermented product with yeast as an active ingredient, and its manufacturing method. In addition, there is a case where the fermented product produced by utilizing lactic acid bacteria and yeast is called a lactic acid bacterium/yeast fermented product. [Raw material for ferment] The raw material of the fermented seasoning composition is pure milk, whey or soymilk derived from cow's milk, preferably whey or soymilk derived from cow's milk. The whey that can be used is not particularly limited as long as it can be used as a food material. Whey, also known as whey, is obtained by removing casein and milk fat, which are the main components of milk protein, from milk, and contains water-soluble protein, lactose, and water-soluble vitamins. or an aqueous solution of mineral components. The milk can also be obtained in the form of a yogurt supernatant, except that it is produced as a by-product when cheese is produced from cow's milk. Whey is supplied to the market in the form of dry powder, and such a dry product can also be preferably used in this embodiment. The solid content concentration of the whey to be used in the fermentation is not particularly limited as long as the fermentation using lactic acid bacteria is preferably carried out. It is 5 to 40%, preferably 10 to 25%, more preferably 12 to 18%. The soymilk that can be used is not particularly limited as long as it can be used as a food material. As soymilk that can be preferably used, various commercially available ones include, for example, unadjusted soymilk (soybean solid content: 8% or more), adjusted soymilk (soybean solid content: 6% or more), soymilk beverage (soybean solid content) 4% or more), dry powder of soymilk. The soybean solid content concentration of the fermented liquid is not particularly limited as long as the fermentation using lactic acid bacteria is preferably carried out. It is preferable to set it as 5 to 20%. The whey and soy milk used in the fermenting can also be heat sterilized in order to prevent the contamination of various bacteria before being supplied to the fermenting. Conditions for heat sterilization are, for example, 80 to 90° C. for 30 to 60 minutes. [Lactic acid bacteria fermentation] When fermenting whey or soy milk, the order of fermentation using lactic acid bacteria and fermentation using yeast can be appropriately set. In a preferred embodiment, the lactic acid bacteria are used first. The fermenting carried out. By sequentially performing the fermentation using anaerobic lactic acid bacteria and the fermentation using yeast while aerating as described below, it is possible to provide better conditions for each bacteria, for example, even if the pH value decreases due to lactic acid bacteria. , and also because the yeast tolerates acid, it can still grow and so on. Moreover, by this, the whole fermentation process can be completed in a short time. Furthermore, the flavor component derived from yeast can fully be utilized. The lactic acid bacteria used are not particularly limited as long as they can be used in food manufacturers. For example, Lactobacillus rhamnosus (Lactobacillus rhamnosus), Pediococcus acidlactici (Pediococcus acidlactici), Lactobacillus confusus (Lactobacillus confusus), Lactobacillus malefermentans, Lactobacillus paracasei subsp. paracasei, Lactobacillus delbrueckii subsp. bulgaricus, Lactococcus lactis, Lactobacillus acidophilus, and Swiss Any of the group consisting of Lactobacillus helveticus and the like. It is preferably selected from Lactobacillus rhamnosus, Pediococcus acidlactici, Lactobacillus confusus, Lactobacillus malefermentans, Lactococcus lactis, and The lactic acid bacteria in the group consisting of Lactobacillus acidophilus is more preferably any one of Lactobacillus rhamnosus or Pediococcus acidlactici. It is preferable to use Lactobacillus rhamnosus from the viewpoint of good growth property and excellent fermented flavor. In a preferred embodiment, as the lactic acid bacteria, Lactobacillus rhamnosus AN1 strain or strains belonging to the same species and having the same scientific properties, or Pleurococcus lactis YU2 strains or strains belonging to the same species and having the same properties can be used. The scientific nature of the strains. The Lactobacillus rhamnosus AN1 strain has the following scientific properties. Cell shape: rod-shaped colony: milky white Features: carry out heterozygous lactic acid fermentation. Lactococcus lactis YU2 strain has the following scientific properties. Cell shape: spherical Colony: milky white Features: Tetradococcus, homogenous lactic acid fermentation. Lactobacillus rhamnosus AN1 strain, and Pediatricoccus lactis YU2 strain, respectively, were successively registered as NITE BP-02072 and NITE BP-02073, on June 26, 2015, by TableMark Co., Ltd. (Address: Tokyo, Japan) 6-4-10 Tsukiji, Chuo-ku), and deposited with the Independent Administrative Incorporated Product Evaluation Technology Base Agency based on the Budapest Treaty and the Japanese Patent Law (Address: Room 122, Kamazuma 2-5-8, Kamazuma, Kisarazu City, Chiba Prefecture, Japan) . The conditions for fermentation using lactic acid bacteria can be appropriately designed by the practitioner according to the lactic acid bacteria to be used. In addition to whey and soymilk, various ones may be added to the culture solution. As the carbon source, for example, any one selected from the group consisting of sugarcane waste molasses, sugar beet waste molasses, sucrose, wood chip cooking liquor, sulfite pulp waste liquor, sugarcane extract, glucose, acetic acid, and ethanol can be used. As the nitrogen source, for example, one selected from the group consisting of nitrogen-containing organic substances such as yeast extract, egg whites, corn steep liquor (CSL), and casein, and inorganic salts such as urea, ammonia, ammonium sulfate, ammonium chloride, and ammonium phosphate can be used. any of the group. Furthermore, phosphoric acid components, potassium components, and magnesium components may be added to the medium, vitamins such as biotin, pantothenic acid, thiamine, inositol, and pyridoxine, and minerals such as zinc, copper, iron, and manganese may be added. kind. Although the lactic acid bacteria fermentation also depends on the strain used, it can be carried out, for example, at 30 to 45°C, preferably 35 to 40°C, for 16 to 48 hours, preferably 20 to 36 hours. Considering that the lactic acid bacteria are anaerobic, the fermentation can be performed while standing still or with light stirring. Moreover, fermenting may be performed until pH value becomes 3.0-4.0, Preferably it is the range of 3.2-3.8. [Yeast fermenting] In a preferred embodiment, the lactic acid bacteria fermented product obtained in the above manner can be subjected to yeast fermenting as the next step. The yeast that can be used for yeast fermentation is not particularly limited as long as it can be used for food manufacturers. For example, conventional yeasts such as Saccharomyces cerevisiae, Saccharomyces cerevisiae, and Saccharomyces cerevisiae can be used. More specifically, for example, it is selected from the genus Saccharomyces, the genus Shizosaccharomyces, the genus Pichia, the genus Candida, the genus Kluyveromyces, Williopsis, Debaryomyces, Galactomyces, Torulaspora, Rhodotorula, Yarrow lipolytica (Yarrowia) and any of the group consisting of Zygosaccharomyces. As for the yeast, in terms of good growth properties, baker's yeast used for bread making, torula yeast used for manufacture of foodstuffs, feed, etc., and brewer's yeast used for brewing beer are more preferable, and those belonging to yeast ( Saccharomyces) or bacteria belonging to Candida. Examples of the genus Saccharomyces include Saccharomyces cerevisiae, Saccharomyces sake, and Saccharomyces beticus. Furthermore, Saccharomyces sake and Saccharomyces beticus are also classified as one type of Saccharomyces cerevisiae. Examples of the genus Candida include Candida tropicalis, Candida lypolitica, Candida utilis, and Candida sake. More preferably, it is any one selected from the group consisting of Saccharomyces cerevisiae, Saccharomyces sake, Schizosaccharomyces pombe, and the like. One of the preferred yeasts is Saccharomyces beticus, which is also regarded as a type of Saccharomyces cerevisiae, mainly including membrane-producing yeasts used in the production of white wine. In a preferred embodiment, as the yeast, Saccharomyces cerevisiae FAHP-1 strain or strains belonging to the same species and having the same scientific properties, or Saccharomyces cerevisiae FAHP-2 strains or belonging to the same species and strains with the same scientific properties. Saccharomyces cerevisiae FAHP-1 has the following scientific properties. Cell shape: egg type Colony: milky white Features: form ascospores with fermenting ability. Saccharomyces cerevisiae FAHP-2 has the following scientific properties. Cell shape: egg type Colony: milky white Features: form ascospores with fermenting ability. Saccharomyces cerevisiae FAHP-1 strain, and Saccharomyces cerevisiae FAHP-2 strain, respectively, under the accession numbers NITE BP-02074 and NITE BP-02075, were registered on June 26, 2015 by TableMark Co., Ltd. (address: 6-4-10 Tsukiji, Chuo-ku, Tokyo, Japan), and deposited with the Independent Administrative Incorporated Product Evaluation Technology Base Agency based on the Budapest Treaty and the Japanese Patent Law (Address: 2-5-8 122 Kamazuma, Kamazuma, Kisarazu City, Chiba Prefecture, Japan) room). The conditions for fermentation using yeast can be appropriately designed depending on the yeast to be used as long as it is a manufacturer. During cultivation, the lactic acid bacteria ferment can also be sterilized. Furthermore, various ones can be added to the lactic acid bacteria fermented product. As the carbon source, for example, any one selected from the group consisting of sugarcane waste molasses, sugar beet waste molasses, sucrose, wood chip cooking liquor, sulfite pulp waste liquor, sugarcane extract, glucose, acetic acid, and ethanol can be used. As the nitrogen source, for example, one selected from the group consisting of nitrogen-containing organic substances such as yeast extract, egg whites, corn steep liquor (CSL), and casein, and inorganic salts such as urea, ammonia, ammonium sulfate, ammonium chloride, and ammonium phosphate can be used. any of the group. Furthermore, phosphoric acid components, potassium components, and magnesium components may be added to the medium, vitamins such as biotin, pantothenic acid, thiamine, inositol, and pyridoxine, and minerals such as zinc, copper, iron, and manganese may be added. kind. Yeast fermentation also depends on the strain used, and can be carried out, for example, at 20 to 40°C, preferably 25 to 35°C, for, for example, 12 to 40 hours, preferably 16 to 24 hours. The fermentation system is carried out under aerobic conditions. If necessary, it can be carried out with sufficient ventilation and/or stirring after the yeast fermentation. As for aeration and/or stirring, specifically, the same amount of sterile air as about 1/4 of the volume of the fermenting liquid can be blown into the bottom of the fermenting tank every 1 minute, and stirring is performed at 100-400 rpm and proceed. Furthermore, in the production process of alcoholic beverages, since fermentation is usually performed under anaerobic conditions, the yeast produces a large amount of ethanol. However, in this embodiment, since the fermentation is performed under aerobic conditions, the ethanol concentration in the fermentation is kept low, and the yeast can generate more various flavor components such as esters, fatty acids, and higher alcohols. The alcohol concentration in the yeast fermentation process and the fermentation liquid after the fermentation is completed is preferably less than 1.0%. The termination point of yeast fermentation can be appropriately determined as long as it is a manufacturer. Brix) value, and flavor, etc., were used as a reference. When the Brix value is used as a standard, when using soymilk as a raw material, it can be 5-9%, preferably 6-8%, and when using whey as a raw material, it can be 9-9%. 13%, preferably 10 to 12%. Here, the so-called Brix value refers to a value obtained by removing insoluble solid content derived from whey or soymilk, lactic acid bacteria cells, and yeast cells from a liquid fermented product value. In the case of adding salt to the starter, the value of the added salt is not included. Furthermore, the Brix value is determined by the value corresponding to the mass percentage of the sucrose solution at 20°C. When measuring 100 g of an aqueous solution containing only 1 g of sucrose as a solute using a sugar refractometer , its reading Brix value is 1%. For solutions containing solid components other than sucrose, the Brix value is used as a standard for the concentration of solid components. An apparatus for measuring the Brix value is commercially available, and the measurement can be appropriately performed as long as it is a manufacturer. [Other steps] Salt may be added to the obtained lactic acid bacteria/yeast fermented product to improve storage stability. The amount of salt added is not particularly limited, but in order to sufficiently reduce water activity, it can be added, for example, at 10% or more, preferably 15% or more, and more preferably 18% or more relative to the lactic acid bacteria/yeast fermentation broth. Furthermore, the obtained lactic acid bacteria/yeast fermented product contains insoluble solid content derived from whey or soymilk, lactic acid bacteria cells, and yeast cells, so when it is intended to be used for products requiring clarity, it is also Clarification treatment can be performed to remove these insoluble components or bacterial cells. Regarding the method of clarification, various methods used in the field of food production for the same purpose can be applied. For example, filter press filtration or microfiltration (MF: Micro filtration) using diatomaceous earth or peritone as a filter aid can be mentioned. [Lactic acid bacteria/yeast starter (active ingredient)] In the above-described manner, lactic acid bacteria/yeast starter is obtained from whey or soymilk as raw materials. The lactic acid bacteria/yeast fermented product is a liquid obtained by removing the insoluble solid content derived from whey or soymilk, lactic acid bacteria cells, and yeast cells obtained from lactic acid bacteria fermentation and yeast fermentation. For example, when whey is used as the raw material, the Brix can be 9 to 13%, preferably 10 to 12%, and when soymilk is used as the raw material, the Brix (Brix) Brix) may be 5 to 9%, preferably 6 to 8%. The lactic acid bacteria/yeast starter can be in various forms. For example, if necessary, the above-mentioned liquid form can be concentrated, dried, or the like to make paste, solid, powder, granule, or the like. The lactic acid bacteria/yeast starter is also called W (double) leaven seasoning, and it is also called simply as W (double) leaven seasoning. If the inventors of the present invention conduct further research, it is found that the following is the case: in the lactic acid bacteria/yeast ferment of whey or soymilk, the components of the components with a molecular weight of 100-5,000, more specifically the components with a molecular weight of 500-3,500 Contributes to the effect of the target. Therefore, according to the present invention, a fermented seasoning composition is provided, the active ingredient of which comprises components differentiated based on the molecular weight of lactic acid bacteria/yeast fermented product, and the molecular weight of the lower limit of the components is 75-1800, preferably 100- 500, even when the lower limit is any one, the molecular weight of the upper limit is 2200-10000, preferably 3500-5000 (for example, a component with a molecular weight of 500-3500, or a component with a molecular weight of 100-5000). In addition, a food texture improving agent is provided, which comprises components of lactic acid bacteria/yeast leaven of whey or soymilk that are differentiated based on molecular weight, and the molecular weight of the lower limit of the components is 75-1800, preferably 100-500 , even when the lower limit is any one, the molecular weight of the upper limit is 2200-10000, preferably 3500-5000 (for example, a component with a molecular weight of 500-3500, or a component with a molecular weight of 100-5000). Regarding the present invention and its embodiments, the so-called molecular weight refers to the molecular weight that can be distinguished using a dialysis tube having molecular distinguishing ability. Regarding the components of the lactic acid bacteria/yeast fermentation products of whey or soymilk with a molecular weight of 500-3,500 (or a component with a molecular weight of 100-5,000), typically, at least the use of a dialysis tube with a molecular weight of 3,500-5,000 (Spectra /Por, Funakoshi) and dialysis tubes (Spectra/Por, Funakoshi) with a molecular weight of 100 to 500 are distinguished by ordinary operations (if further specified, the conditions described in item 8 of Example 8 of this specification) are obtained. the components. In addition, the molecular weight cut off (MWCO) of the dialysis tube listed here means that the component which maintains this size is 90% or more. As one of the embodiments of the present invention, the lactic acid bacteria/yeast fermented product obtained by further fermenting the lactic acid bacteria/yeast fermented product using soymilk as a raw material, preferably the lactic acid bacterium fermented product of soymilk, using yeast is novel. [Yeast seasoning composition] The obtained lactic acid bacteria/yeast starter can be used as an active ingredient of a fermented seasoning composition. The fermented seasoning composition can also be the lactic acid bacteria/yeast fermented product itself, and other ingredients can be formulated as long as the active ingredient can exert the desired effect. Other ingredients can be various additives that are allowed as food. In this example, antioxidants, fragrances, seasonings, sweeteners, colorants, tackifiers, stabilizers, color developers, bleaching agents, antifungal agents, gums, bitters, etc., enzymes, and glossing agents , acidulant, emulsifier, fortifier, manufacturing agent, excipient, binder, tensioning agent (isotonic agent), buffer, dissolution aid, preservative, stabilizer, coagulant, etc. The amount of the active ingredient in the fermented seasoning composition is, for example, 1 to 100%, 10 to 80%, or 20 to 60%. The fermented seasoning composition is particularly suitable for improving the texture of food, softening meat, etc., masking the fishy smell of soybean milk, masking the odor of vegetable protein, or enhancing the irritation of spices as follows. The food composition can also be referred to as "food texture improving agent", "softening agent for animal meat, etc.", "masking agent for fishy odor of soybean milk", "masking agent for vegetable protein odor", "stimulating agent for spice". Enhancer, Cocoa Flavor Enhancer, Suppressor of Light Deterioration Odor of Milk or Soy Milk, Milky Flavor Enhancer, Miso Grain Flavor Enhancer, Fish Odor Masking Agent, Livestock Meat Odor masking agent, "fruitiness enhancer", and "natto odor masking agent". That is, the present invention also provides the following. [1a] A food texture improver, a softener for animal meat, etc., a masking agent for the fishy odor of soybean milk, a masking agent for the odor of vegetable protein, an enhancer for the irritating feeling of spices, a cocoa flavor enhancer, and the light of milk or soybean milk Deterioration odor inhibitor, milk texture enhancer, miso cereal flavor enhancer, fish odor masking agent, animal meat odor masking agent, fruit flavor enhancer, or natto odor masking agent, which uses lactic acid bacteria/yeast of whey or soymilk Enzyme as an active ingredient. [2a] The agent as described in 1a, wherein the lactic acid bacteria are selected from Lactobacillus rhamnosus, Pediococcus acidlactici, Lactobacillus confusus, Lactobacillus malefermentans, Lactobacillus paracasei subsp. paracasei, Lactobacillus delbrueckii subsp. bulgaricus, Lactococcus lactis, Lactobacillus acidophilus, and Lactobacillus helveticus any of the group. [3a] The agent according to 1a or 2a, wherein the yeast is selected from Saccharomyces cerevisiae, Saccharomyces sake, Saccharomyces beticus, Schizosaccharomyces pombe ) in any of the groups. [4a] The agent according to any one of 1a to 3a, which uses, as an active ingredient, a lactic acid bacteria/yeast fermented product obtained by further fermenting a lactic acid bacteria fermented product of soybean milk. [Application] <Improvement of texture> The obtained lactic acid bacteria/yeast fermented product or fermented seasoning composition can be used to improve the texture of food. The so-called material texture means that the taste and/or food texture (texture) peculiar to the ingredients contained in the food can be clearly felt. Food contains various ingredients, and the ingredients are mixed with each other during heating and conditioning, and the taste is enhanced to enhance the overall sense of the food as a whole. At this time, the taste of blending or the softness is felt well, but when the heat-prepared food is cooled and stored at a low temperature, the overall feeling or the taste will increase to an unintended level. The taste or texture peculiar to each ingredient is greatly lost, and it becomes difficult to discriminate each ingredient in the mouth when eating. The inventors of the present invention conducted research and found that by adding lactic acid bacteria/yeast ferment, even if the food is stored in refrigeration or freezing for cooling after heating and conditioning, the texture of the ingredients can be maintained more than before. Therefore, one aspect of the present invention is an improver for the taste of food materials containing the obtained lactic acid bacteria/yeast fermented product or fermented seasoning composition as an active ingredient. <Softening of livestock meat, etc.> The obtained lactic acid bacteria/yeast fermented product or fermented fermented seasoning composition can be used to make livestock meat or fish meat (including processed products using these as raw materials. Fish meat includes aquatic animal meat. There are also When these are collectively referred to as "livestock meat, etc.") softening. Therefore, one aspect of the present invention is a softener for livestock meat, etc., which contains the obtained lactic acid bacteria/yeast starter or fermented seasoning composition as an active ingredient. The softener of the present invention may be contained in a seasoning liquid such as a soaking liquid and injected (injected) into livestock meat or the like. Alternatively, it can also be used by immersing livestock meat or the like in a dipping solution containing the softener of the present invention. Alternatively, it may be contained in foods such as hamburger patties. By using the softener of the present invention, meat and the like are not only softened, but also the juiciness can be enhanced. The juicy feeling here refers to the juicy feeling in the mouth when chewing animal meat or the like at the time of eating. <Soymilk smell masking> The obtained lactic acid bacteria/yeast fermented product or fermented seasoning composition can also be used to suppress the fishy smell of soymilk (including processed products using it as a raw material). Therefore, one aspect of the present invention is a masking agent for the fishy smell of soymilk containing a lactic acid bacteria/yeast starter or a starter seasoning composition as an active ingredient. The object of the fishy smell masking of the present invention is not limited to soymilk, but can also be processed products using soymilk as a raw material, such as soymilk cream. By using the masking agent of the present invention, not only the fishy smell of soybean milk is masked, but also the sweetness, softness and thickness of taste can be enhanced. <Plant protein odor masking> Vegetable protein, especially soybean protein (including processed products from it) has unique oxidation odor, but the obtained lactic acid bacteria/yeast fermented product or fermented seasoning composition can be used to suppress this Plant protein smell (also known as "plant protein smell"). Therefore, one aspect of the present invention is a masking agent for the odor of vegetable protein containing a lactic acid bacteria/yeast starter or a starter seasoning composition as an active ingredient. The lactic acid bacteria/yeast fermented product or fermented seasoning composition of the present invention has a very weak taste or flavor, so that the taste or flavor of the material can be used while masking the odor of vegetable protein. By using the masking agent of the present invention, not only the oxidative taste of vegetable protein is masked, but also the softness, juiciness and fleshiness can be improved. <Enhanced Sensation of Spices> The obtained lactic acid bacteria/yeast starter or fermented seasoning composition can be used to enhance the stimuli of spices (including processed products made of them) contained in cooking or seasoning liquids. That is, by adding the lactic acid bacteria/yeast fermented product or fermented seasoning composition of the present invention to a seasoning liquid or various sauces containing spices, the stimulation of the spices can be more strongly felt when eating, and the Stimulate long-term retention. Therefore, one aspect of the present invention is an enhancer for the pungent sensation (also referred to as "spice sensation") of a spice containing a lactic acid bacteria/yeast starter or a fermented seasoning composition as an active ingredient. Spices are those having the effect of changing taste, aroma, color, richness, ripeness, or stimulation (spicy) by adding them to foods, thereby providing a delicious taste or an increase in appetite. The spice that enhances the sense of stimulation by the present invention is not limited, but includes black pepper, red pepper, green pepper, pepper, cinnamon, nutmeg, cloves, allspice, cumin, dried tangerine peel, coriander, anise, and rat tail Grass, thyme, bay, jasmine, curry leaves, guava, sansho, chili, mustard, ginger, garlic, chili powder, onion, leek, sesame, five-spice powder, three spices, curry powder, chili powder, five-spice pepper powder, etc. By using the enhancer of the present invention, not only the pungent sensation of the spice is enhanced, but also the mature sensation, the richness, and the aftertaste can be enhanced. <Cocoa Flavor Enhancement> The obtained lactic acid bacteria/yeast starter or fermented seasoning composition can be used to enhance the flavor of cocoa in foods containing cocoa-derived raw materials. That is, by adding the lactic acid bacteria/yeast fermented product or fermented seasoning composition of the present invention to a food containing a cocoa-derived raw material, the flavor of cocoa can be more strongly felt when eating. Therefore, one aspect of the present invention is a cocoa flavor enhancer in a food containing a cocoa-derived raw material containing a lactic acid bacteria/yeast starter or a starter seasoning composition as an active ingredient. Cocoa flavor is also known as one of the flavors of chocolate (cocoa flavor, beer flavor, milk flavor). The raw material derived from cocoa is not limited, and examples thereof include cocoa nibs, cocoa mass, cocoa liquor, cocoa powder, cocoa cake, cocoa butter, and chocolate. <Suppression of Light Deterioration Odor> The obtained lactic acid bacteria/yeast fermented product or fermented seasoning composition can be further used to inhibit milk (including processed products using the same) or soybean milk (including processed products using the same as raw materials) ) light deterioration odor. Photodegradation odor refers to gas generated by oxidation caused by sunlight or the like. The light-degraded odor suppression may be the suppression of oxidative deterioration caused by light, or may be the shielding of the odor generated by light-deterioration. One aspect of the present invention is a photo-deterioration odor inhibitor of milk or soymilk containing a lactic acid bacteria/yeast fermented product or a fermented seasoning composition as an active ingredient. <Improvement of milky feeling> The obtained lactic acid bacteria/yeast fermented product or fermented seasoning composition can be used to improve the milky feeling. That is, by adding the lactic acid bacteria/yeast fermented product or fermented seasoning composition of the present invention to a food using cow's milk (including processed products using it as a raw material), the milk can be more strongly felt when eating. feel. Therefore, one aspect of the present invention is a milky feeling enhancer containing a lactic acid bacteria/yeast starter or a starter seasoning composition as an active ingredient. The so-called improvement of milky feeling refers to the improvement of the good flavor peculiar to cow's milk. There is no limitation on the food that is the object of improving the milky feeling, and it is the milk or the dairy product itself, and the food using the same as a raw material. <Enhancement of cereal flavor of miso> The obtained lactic acid bacteria/yeast starter or fermented seasoning composition can be used to enhance the cereal flavor (soybean flavor, rice flavor, or wheat flavor) of miso. That is, by adding the lactic acid bacteria/yeast fermented product or fermented seasoning composition of the present invention to a miso-containing food, the cereal flavor of miso can be more strongly felt when eating. Therefore, one aspect of the present invention is a cereal flavor enhancer for miso containing a lactic acid bacteria/yeast starter or a starter seasoning composition as an active ingredient. The miso is not limited, and examples thereof include white miso, red miso, mixed miso, rice miso (obtained by fermenting soybeans and rice, and ripening), wheat miso (fermenting soybeans and barley or rye, Aged and obtained), soybean miso (obtained by fermenting and aging soybeans), blended miso (obtained by mixing each of rice miso, wheat miso, and bean miso), etc. Preferred examples are white miso and blended miso. With regard to miso that is usually pasteurized by heat or miso that is freeze-dried, such as seasoning miso for instant miso juice, it is found that the flavor of miso tends to deteriorate, and the lactic acid bacteria/yeast fermented or fermented seasoning composition of the present invention may be preferred It is used to enhance the grain flavor of this miso. The miso grain flavor enhancer of the present invention can be applied to various foods containing miso. By using the miso grain flavor enhancer of the present invention, not only the grain flavor of miso but also sweetness and/or umami can be improved. <Fish odor masking> The obtained lactic acid bacteria/yeast fermented product or fermented fermented seasoning composition can be further used to suppress fish odor. One aspect of the present invention is a fish odor inhibitor containing a lactic acid bacteria/yeast fermented product or a fermented fermented seasoning composition as an active ingredient. The odor of fish meat includes the unpleasant odor (fishy smell) of raw fish and the peculiar smell (fish odor) of heated fish. The food subject to the suppression of fish odor is not limited to the fish itself, but may also be processed products using fish as a raw material, or foods using such. <Livestock odor masking> The obtained lactic acid bacteria/yeast fermented product or fermented yeast seasoning composition can be further used to suppress livestock meat odor. Meat odor includes visceral odor, grass odor, and animal odor. The so-called grass odor refers to the unique grass smell of beef obtained from cattle that have been fattened by grass. One aspect of the present invention is a livestock meat odor inhibitor containing a lactic acid bacteria/yeast fermented product or a fermented fermented seasoning composition as an active ingredient. The object of the livestock meat odor inhibitor of the present invention is not limited to livestock meat, and may be processed products or foods containing these as raw materials. Furthermore, the above-mentioned Patent Document 30 proposes to use arginine to reduce the odor of livestock meat, but the above-mentioned Patent Document 29 points out the problem that since arginine itself exhibits a bitter taste, it also imparts a bitter taste to the livestock meat. According to the present invention, it is considered that even if an effective amount of the active ingredient of the present invention is used, there is no such problem. <Improvement of Fruit Feeling> The obtained lactic acid bacteria/yeast fermented product or fermented seasoning composition can be used to enhance the fruity feeling of tomatoes or fruits (including food products using these as raw materials). The so-called improvement of fruitiness refers to the improvement of a good flavor similar to tomato (or fruit). One aspect of the present invention is a fruitiness enhancer for a fruit-derived food containing a lactic acid bacteria/yeast starter or a starter seasoning composition as an active ingredient. A particularly good example of the target food is one prepared by heating it by stewing for a long time. According to the present invention, even in such a stewed and heated food, the fruitiness can be improved. By the present invention, not only the fruitiness of tomatoes or fruits can be improved, but also the sour taste can be improved. Usually, if only the sourness is enhanced, the taste of the food tends to be unsatisfactory, but in the case of the present invention, the tomato or the fruit has a better overall taste when the sourness is improved at the same time as the fruitiness. <Natto odor masking> The obtained lactic acid bacteria/yeast fermented product or fermented fermented seasoning composition can also be used to suppress the natto odor of natto (including processed products using it as a raw material). One aspect of the present invention is a natto odor inhibitor of natto containing a lactic acid bacteria/yeast fermented product or a fermented fermented seasoning composition as an active ingredient. When used to suppress the odor of natto, the lactic acid bacteria/yeast fermented substance or the fermented fermented seasoning composition can also be directly added to natto, and can also be contained in the seasoning juice of natto, and mixed with natto when eating. way to use. <Evaluation method and criteria> Whether the texture is improved, and the degree of improvement of the texture; whether the meat, etc. is softened, and the degree to which it should be softened; whether the fishy smell of soymilk is masked, and the degree to which it should be masked; the odor of vegetable protein Whether it is shielded, and the degree of shielding; whether the stimulation of spices is enhanced, and the degree to which it should be enhanced; whether the flavor of cocoa is enhanced, and the degree to which it should be enhanced; whether the light deterioration odor of milk or soymilk is suppressed, and the degree to which it should be suppressed degree; whether the milky feeling is improved, and the degree to which it should be improved; whether the grain flavor of miso is improved, and the degree to which it should be improved; whether the odor of fish meat is masked, and the degree to which it should be masked; whether the odor of animal meat is masked, and the degree to which it should be masked The degree to which the fruitiness has been improved, and the degree to which it should be improved, and whether the natto odor has been masked, and the degree to which it should be masked, as long as it is a business operator, it is possible to plan a sensory test based on an appropriate control food for evaluation. More specifically, for example, prepare a suitable comparison such as foods that do not contain active ingredients, and define a standard of about 3 to 10, based on the standard (industrially meaningful standard, for example, the 7th level or above is defined as a pass. ), a trained sensory examiner compares the subject food to a control, thereby allowing evaluation. Regarding the improvement of the texture, when the food contains a plurality of ingredients, it is preferable to improve the texture of the more and more ingredients. The lactic acid bacteria/yeast fermented product or the fermented seasoning composition of this embodiment can improve not only the texture of vegetables, but also the texture of meat and aquatic animals. The softening of meat and the like can also be evaluated by a method of measuring the hardness of food. In the softening of livestock meat, etc., it is preferable to enhance the juiciness at the same time. In the shielding of the fishy smell of soy milk, it is better to enhance the sweetness, softness and heaviness at the same time. In the masking of protein odor, it is preferable to enhance the softness, juiciness and fleshiness at the same time. In the enhancement of the stimulating sensation of the spice, it is preferable to enhance the richness, ripeness and aftertaste at the same time. As a method for a more specific evaluation, the method described in the section of Examples of this specification can be referred to. This application provides for the first time the following application, that is, the lactic acid bacteria/yeast starter of whey or soymilk can be used to improve the texture of food. The above-mentioned Patent Document 1 relates to a lactic acid bacteria/yeast fermented liquid of whey, but does not mention a food for cooling or freezing after heating and conditioning. In addition, the above-mentioned Patent Document 2 describes that by adding whey-based lactic acid bacteria and yeast fermented liquid to fruit beverages, the richness and flavor of the fruit itself are enhanced and the ripe flavor is imparted, but it does not mention that it is used in heating. Refrigerated or frozen food that is cooled after conditioning. In addition, the following purposes are also provided for the first time by this application, that is, the lactic acid bacteria/yeast fermented product of whey or soymilk can be used for softening of meat, etc., shielding of the smell of soymilk, shielding of the odor of vegetable protein, enhancement of the stimulation of spices, Cocoa flavor enhancement, photo-deterioration odor suppression of milk or soymilk, milk texture enhancement, miso-grain flavor enhancement, fish odor masking, animal meat odor masking, fruity flavor enhancement, and natto odor masking. In addition, the lactic acid bacteria/yeast starter or the fermented seasoning composition obtained by this embodiment can be used to enhance the salty taste of a specific food. As for whether or not the salty taste is enhanced, and the degree of the enhancement of the salty taste, as long as it is a business operator, it is possible to plan a sensory test using an appropriate control food as a standard for evaluation. [Applied food] The lactic acid bacteria/yeast fermented product or fermented seasoning composition obtained by this embodiment can be applied to various foods. Preferred examples of the food to be used are refrigerated or frozen refrigerated food and frozen food, livestock meat or fish meat (including processed products using these as raw materials. Fish meat includes aquatic animal meat. There are also cases where these are collectively referred to as "livestock meat, etc."), soybean milk (including processed products from them), vegetable proteins (including processed products from them), and spices (including processed products from them). processed products), foods containing raw materials derived from cocoa, milk (including processed products from them), miso (including processed products from them), tomatoes (including processed products from them), fruits (including processed products using it as a raw material), and natto (including processed products using it as a raw material). Examples of refrigerated foods and frozen foods include spring rolls, Chinese rice bowls (ingredients), dumplings, shumai, xiaolongbao, pork buns, eight treasures vegetables, double-cooked pork, shredded green peppers, fried vegetables, and fried shredded burdocks. , Chikuzen boiled, in addition to this, can also include: fried products such as beef hash browns, croquettes, fried shrimp cakes; meat products such as hamburger patties; sauced udon noodles, Nagasaki chowder, fried noodles, Neapolitan style Pasta, baked pasta, lasagna and other noodles; fried rice, risotto, risotto, assorted sushi and other rice products; Japanese pancakes, takoyaki, pizza and other wheat flour products. Examples of livestock meat etc. are not particularly limited, but include beef, chicken, pork, horse meat, mutton, goat, cod, salmon and other white fish; tuna, bonito and other naked fish; clams, squid, octopus, shrimp, Crab etc. Examples of processed products using meat or fish as raw materials are not particularly limited, and various softeners are preferred. Western fried pancakes, fried pork chops, fried chicken chops, fried steak, fried shrimp cakes, meat for barbecue, meat for steak, meat for skewers, meat for stew, ham, bacon, etc. Examples of processed products using soymilk as a raw material are not particularly limited, and those that are suitable for masking various fishy smells can be mentioned. Bread, distraction, etc. As examples of processed products using vegetable protein as raw materials, various unique oxidative flavors can be better masked. sausage etc. More preferably, it is a processed product using soybean protein as a raw material. Examples of processed products using spice as a raw material are not particularly limited, and various types of spices are preferred for enhancing the sense of spice. Thick Sauce, Tonkatsu Sauce, Steak Sauce, Barbecue Sauce, Barbecue Sauce, Herb Salt, etc. It does not specifically limit as an example of the foodstuff containing the raw material derived from cocoa, A Japanese-style snack, a baked snack, a bread, a drink, a frozen dessert, a pre-processed food, a seasoning, etc. are mentioned. It does not specifically limit as an example of the food which becomes the object of suppression of light deterioration odor, Fresh milk, cow's milk, ingredient-adjusted milk, low-fat milk, non-fat milk, processed milk, cream, butter, milk fat, cheese , Concentrated whey, ice cream (ice cream, ice milk, lactic acid ice cream, etc.), concentrated milk, skimmed concentrated milk, sugar-free condensed milk, sugar-free condensed skim milk, sweetened condensed milk, sweetened condensed skim milk, whole milk, skimmed milk, custard powder, Whey powder, protein concentrate whey powder, buttermilk powder, sweetened powdered milk, adjusted powdered milk (milk powder for childcare, etc.), milk drink, fermented milk, and lactic acid bacteria drink. Particularly preferred examples of the food to be targeted include soybean milk, cow's milk, and cream. It does not specifically limit as another example of the food which becomes the object of improving milky texture, It can be mentioned: yoghurt, ice cream, puffs, creme brulee, flower cake, white sauce, cream sauce, creamy beef and potato Cakes, gratin cheese gratin, and fish and shellfish chicken rice, etc. There is no particular limitation as an example of the food to be used to enhance the grain flavor of miso, and in addition to the miso itself, instant miso juice and miso paste can be exemplified. It does not specifically limit as an example of the food which becomes the object of masking the smell of fish, but includes grilled fish, processed fish products (fish sausage, fish sauce, kamaboko, cylindrical kamaboko, fish potato cake, etc.), fish Processed products (dry goods, wet delicacies, boiled fish, sliced fish marinated with Xijing miso, canned fish, etc.), fish balls, aquatic animal extracts, bonito rice. There are no particular limitations on examples of foods that can be used to mask the odor of animal meat, and examples include: meat-prepared products (products or semi-processed products made from beef, pork, or other meat as raw materials, which are heat-prepared or seasoned) , Specifically, beef seasonings (beef curry, beef jerky, canned salted beef, roast beef, seasoned beef, etc.), pork seasonings (seasoned pork, sausages, canned ham, pork luncheon meat, etc.), hamburgers Pie, meat for steak, meat for barbecue (beef tongue, diaphragm, liver, etc.), ham, sausage, bacon, seasoned pork ribs, beef bowl, pork bowl, chop suey, hamburger patty, deep-fried Pork chops, ancient meats, pork tempura, dumplings, American pork stew. Foods to be used to enhance the sense of fruit are tomatoes and fruits (including processed products as raw materials). Fruits are not particularly limited, but include citrus fruits, such as lemons, grapefruits (white pulp type, red pulp type), limes , oranges (Navel Orange, Valencia Orange), Wenzhou mandarin, Tangor, summer mandarin, Ganxia, Hassaku mandarin, Hyuga summer orange, flat lemon, sour mandarin, Grapefruit, stinky oranges, yellow-skinned limes, Iyo mandarin oranges, citrus mandarins, kumquats, sambo mandarins, platinum grapefruit, pomelo; stone fruits such as apricots (alias apricots), cherries, plums, plums (Japanese plums, plums ), peaches (peaches, nectarines, yellow peaches); berries such as grapes (muscadines, Rieslings, Delaware, Kyoho), bananas, blackberries, blueberries, raspberries, gooseberries (alias gooseberry), pomegranate, apple, pears (Japanese pear, Chinese pear, Western pear), pineapple, kiwi, pineapple, passion fruit, West Indies cherries, lychees, mutong, honeydew, avocado, figs, Olive, persimmon, horned melon, guava, lilac, coconut, carambola (alias Star fruit), orange pomelo, custard apple, durian fruit, jujube, date palm, blueberry, papaya, dragon fruit, loquat, longan, Fragrant flesh fruit, melon, quince, mango, mangosteen, bayberry. In addition, the fruit includes fruit-type vegetables, that is, strawberries, watermelons, and melons. As a preferable example of the foodstuff which becomes the object of improving the fruitiness, tomatoes, strawberries, apples, oranges, tangerines, blueberries, mangoes, figs, and peaches can be mentioned. As a more preferable example, tomato sauce, fruit sauce, and jam prepared by stewing and heating for a long time can be mentioned. Foods that are targeted for the odor of natto are natto and its processed products. Examples of processed products are not particularly limited, including sauteed natto, pancakes with natto, and hamburger meat with natto. Cake, meat sauce with natto, sushi with natto, soup with natto, pancake with natto, tempura with natto, multi-colored noodles with natto, noodle soup with natto. Although the amount of lactic acid bacteria/yeast starter added to food also depends on the type of food, typically, in the case of lactic acid bacteria/yeast starter using whey as a raw material, it is converted to solid content of 10.7 with respect to food. % of lactic acid bacteria/yeast starter, in the case of lactic acid bacteria/yeast starter using soymilk as raw material, it is 0.1% or more, preferably 0.13% or more, converted to lactic acid bacteria/yeast starter with a solid content of 6.5%, More preferably, it is 0.17% or more, and still more preferably 0.2% or more. The reason for this is that if it is less than 0.1%, the effect is insufficient. In order to mask the fishy smell of soybean milk, it is especially preferable to set it as 0.4% or more. The upper limit of the added amount is not limited as long as the acidity or aroma of the lactic acid bacteria/yeast fermented product does not impair the flavor of the target food. Although the upper limit of the added amount also depends on the food, if it exceeds 2%, the flavor of lactic acid bacteria/yeast leaven will be felt, so it is preferably 2.0% or less, and can also be 1.8% or less, or 1.5%. the following. It can also be set to 1.0% or less with respect to foods with a weak taste, such as soybean milk, which is easy to feel off-flavor or astringency, and it is possible to obtain a sufficiently high effect without feeling off-flavor and astringency. In other words, it can also be set to 0.7% or less. [Others] An embodiment of the present invention is a method for producing refrigerated food or frozen food, comprising the steps of: refrigerating or refrigerating the above-described heat-conditioned food containing an effective amount of lactic acid bacteria/yeast leaven. Frozen to obtain refrigerated or frozen food. Or a kind of manufacture method of the food that comprises animal meat or fish meat, soybean milk, vegetable protein, spice, raw material derived from cocoa, milk, miso, tomato or fruit, or natto in raw material, it comprises the steps: adding lactic acid bacteria/yeast fermented product the effective amount. The addition stage of the lactic acid bacteria/yeast fermented product in the production method of food can be appropriately set in consideration of workability and the like. Here, the so-called adding includes injection and contact therewith. For example, in order to soften livestock meat, etc., the addition may be a case where a softener is contained in a seasoning liquid such as a dipping liquid and injected into the meat etc. This is the case where softeners are added to raw materials or intermediate products and mixed. As another embodiment of the present invention, a method for improving the texture of refrigerated food or frozen food can be provided, which comprises the following steps: adding the above-described heat-conditioned food containing an effective amount of lactic acid bacteria/yeast leaven. Refrigerated or frozen to obtain refrigerated food or frozen food; or a kind used for softening meat or fish, masking fishy odor of soybean milk, masking odor of vegetable protein, enhancing the stimulation of spices, enhancing the flavor of cocoa, and light-deteriorating odor of milk or soybean milk A method for inhibiting, improving milky feeling, improving the grain flavor of miso, masking fish odor, masking animal meat odor, improving fruit feeling, or masking natto odor, comprising the steps of: adding an effective amount of lactic acid bacteria/yeast leavening; A method for producing a fermented seasoning composition with lactic acid bacteria/yeast starter as an active ingredient, which comprises the following steps: using yeast to further ferment the lactic acid bacteria starter of whey or soymilk. [Examples] Next, the present invention will be described in more detail with reference to Examples and Comparative Examples, but the scope of the present invention is not limited to the following Examples. [Example 1] Preparation of clarified salt-added lactic acid bacteria and yeast whey fermented liquid The preparation is shown below. [Table 1] (1) Composition of lactic acid bacteria seed medium Raw material name Allocated amount Notes skim milk powder 7.00 g Morinaga Skim Milk yeast extract 0.07 g Difco: Bacto Yeast Extract water 63.00 g distilled water (2) Composition of yeast seed medium Raw material name Allocated amount Notes glucose 0.70g Wako Pure Chemicals: Premium yeast extract 0.35g Difco: Bacto Yeast Extract soy peptide 0.35g Fuji Oil: HI-NUTE DC6 water 68.60g distilled water (3) Raw material of lactic acid fermented liquid Raw material name Allocated amount Notes Whey Powder 300 g Four Leaf Dairy yeast extract 1.2g Difco: Bacto Yeast Extract water 1700 g distilled water (4) Additional raw materials for yeast fermented liquid Raw material name Allocated amount Notes glucose 30.0 g Wako Pure Chemicals: Premium yeast extract 2.0g Difco: Bacto Yeast Extract water 68.0 g distilled water ・Lactic acid bacteria seed culture According to the above (1), each raw material of the lactic acid bacteria seed medium was mixed in a 100 ml Erlenmeyer flask and dissolved, then heat sterilized at 85°C for 30 minutes, and cooled to 36°C. The lactic acid bacteria Pediococcus lactis YU2 strain was inoculated into the cooled medium from the MRS agar medium with a platinum inoculation loop, and then statically cultured at 36° C. for 24 hours to obtain 70 g of a lactic acid bacteria seed culture liquid. ・Yeast seed culture According to the above (2), each raw material of the yeast seed medium was mixed in a 500 ml conical flask with baffles and dissolved, then autoclaved at 120°C for 20 minutes, and cooled to 30°C. Saccharomyces cerevisiae FAHP-2 strain was inoculated into the cooled medium from a YPD agar medium with a platinum inoculation loop, followed by shaking culture (rotation speed: 200 rpm) at 30° C. for 24 hours to obtain 70 g of a yeast seed culture liquid. ・Lactic acid bacteria main fermentation According to the above (3), each raw material was mixed and dissolved in a 3-liter mini-culture tank (manufactured by ABLE), then heat sterilized at 85°C for 30 minutes, and cooled to 36°C. After inoculating 70 g of the above-mentioned lactic acid bacteria seed culture liquid into the cooled liquid, 2070 g of lactic acid bacteria whey fermentation liquid was obtained by performing lactic acid fermentation at 36° C. for 24 hours while stirring at a slight speed at 50 rpm. ・Yeast main fermentation According to the above (4), each raw material was mixed and dissolved in a 100 ml Erlenmeyer flask, and then autoclaved at 120° C. for 20 minutes. After cooling to 30°C, add it to the lactic acid bacteria whey ferment solution in a 3-liter small culture tank whose temperature has been adjusted to 30°C, inoculate 70 g of the above yeast seed culture solution after stirring and mixing, and carry out at 30°C. 2100 g of lactic acid bacteria/yeast whey fermented liquid was obtained by aerating and stirring fermentation for 20 hours (stirring speed: 400 rpm, aeration rate: 1.0 liter/min (0.5 vvm)). Furthermore, in the production steps of alcohol and the like, since fermentation is usually carried out under anaerobic conditions, the yeast produces a large amount of ethanol, but in this example, the fermentation was carried out under aerobic conditions by stirring and aeration. Therefore, the ethanol concentration in the fermentation is always kept below 1.0%, and the yeast mainly produces various flavor components such as esters, fatty acids, and higher alcohols. ・Salt addition step In order to improve the preservability of the lactic acid bacteria/yeast whey fermented liquid, salt was added to the final product so as to be 20%, and it was dissolved while stirring to obtain a salted lactic acid bacteria/yeast whey fermented liquid. ・Filter step In order to improve the clarification, the salt-added lactic acid bacteria/yeast whey fermented liquid was filtered using a precision filtration membrane (MF) to obtain a clarified salted lactic acid bacteria/yeast whey fermented liquid. As MF, Microza USP-143 (nominal pore diameter: 0.1 μm) manufactured by Asahi Kasei Chemical Co., Ltd. was used. ・Sterilization step It was kept in a constant temperature water tank at 85°C for 30 minutes to carry out heat sterilization, and 2000 g of sterilized clarified salt-added lactic acid bacteria/yeast whey fermented liquid (inventive product 1) was obtained. ・Analysis procedure Various analyses were performed on the obtained invention product 1. The results are shown in the following table. [Table 2] Analysis Project Analysis value Analytical method moisture 70.0% 105℃, 4 hr drying method Brix 30.7% Refractometer pH 4.0 pH meter salt 20.0% potentiometric titration General bacterial count Below 10 cfu/g Standard agar medium method coliform bacteria feminine BGLB method heat-resistant bacteria Below 10 cfu/g Standard agar medium method (100°C for 10 minutes) Mold / Yeast Below 10 cfu/g potato dextrose agar method [Example 2] Preparation of clarified salt-added lactic acid bacteria/yeast soybean milk fermented liquid The allocation is shown below. [table 3] (1) Composition of lactic acid bacteria seed medium Raw material name Allocated amount Notes soy milk 68.25g Ji Wen: Soymilk without adjustment of ingredients glucose 1.40g Wako Pure Chemicals: Premium yeast extract 0.35g Difco: Bacto Yeast Extract (2) Composition of yeast seed medium Raw material name Allocated amount Notes glucose 0.70g Wako Pure Chemicals: Premium yeast extract 0.35g Difco: Bacto Yeast Extract soy peptide 0.35g Fuji Oil: HI-NUTE DC6 water 68.60g distilled water (3) Raw material of lactic acid fermented liquid Raw material name Allocated amount Notes soy milk 1950g Ji Wen: Soymilk without adjustment of ingredients glucose 40g Wako Pure Chemicals: Premium yeast extract 10g Difco: Bacto Yeast Extract (4) Additional raw materials for yeast fermented liquid Raw material name Allocated amount Notes glucose 30.0 g Wako Pure Chemicals: Premium yeast extract 2.0g Difco: Bacto Yeast Extract water 68.0 g distilled water ・Lactic acid bacteria seed culture According to the above (1), each raw material of the lactic acid bacteria seed medium was mixed in a 100 ml Erlenmeyer flask and dissolved, then heat sterilized at 85°C for 30 minutes, and cooled to 36°C. The lactic acid bacteria Lactobacillus rhamnosus AN1 strain was inoculated into the cooled medium with platinum inoculation loops from the MRS agar medium, and then statically cultured at 36° C. for 24 hours to obtain 70 g of a lactic acid bacteria seed culture liquid. ・Yeast seed culture According to the above (2), each raw material of the yeast seed medium was mixed in a 500 ml conical flask with baffles and dissolved, then autoclaved at 120°C for 20 minutes, and cooled to 30°C. Saccharomyces cerevisiae FAHP-1 strain was inoculated into the cooled medium from a YPD agar medium with a platinum inoculation loop, followed by shaking culture (rotation speed: 200 rpm) at 30° C. for 24 hours to obtain 70 g of a yeast seed culture liquid. ・Lactic acid bacteria main fermentation According to the above (3), each raw material was mixed and dissolved in a 3-liter mini-culture tank (manufactured by ABLE), then heat sterilized at 85°C for 30 minutes, and cooled to 36°C. After inoculating 70 g of the above-mentioned lactic acid bacteria seed culture liquid into the cooled liquid, 2070 g of lactic acid bacteria soybean milk fermentation liquid was obtained by performing lactic acid fermentation at 36° C. for 24 hours while stirring at a slight speed at 50 rpm. ・Yeast main fermentation According to the above (4), each raw material was mixed and dissolved in a 100 ml Erlenmeyer flask, and then autoclaved at 120° C. for 20 minutes. After cooling to 30°C, add it to the lactic acid bacteria soymilk fermented liquid in a 3-liter small culture tank whose temperature has been adjusted to 30°C, inoculate 70 g of the above-mentioned yeast seed culture liquid after stirring and mixing, and carry out 20 at 30°C. 2100 g of lactic acid bacteria/yeast soybean milk fermentation liquid was obtained by stirring and stirring the fermentation for 1 hour (stirring speed: 400 rpm, ventilation rate: 1.0 liter/min (0.5 vvm)). ・Salt addition step In order to improve the preservation of the lactic acid bacteria/yeast soymilk fermented liquid, salt was added to the final product at a concentration of 20%, and the mixture was dissolved while stirring to obtain a salt-added lactic acid bacteria/yeast soymilk fermented liquid. ・Filter step In order to improve the clarification, the salt-added lactic acid bacteria and yeast soymilk fermented liquid is filtered with a precision filtration membrane (MF) to obtain a clarified salted lactic acid bacteria and yeast soymilk fermented liquid. As MF, Microza USP-143 (nominal pore diameter: 0.1 μm) manufactured by Asahi Kasei Chemical Co., Ltd. was used. ・Sterilization step It was kept in a constant temperature water tank at 85°C for 30 minutes to carry out heat sterilization to obtain 2000 g of sterilized clarified salt-added lactic acid bacteria-yeast soybean milk fermented liquid (inventive product 2). ・Analysis procedure Various analyses were performed on the obtained invention product 2. The results are shown in the following table. [Table 4] Analysis Project Analysis value Analytical method moisture 78.20% 105℃, 4 hr drying method Brix 27.40% Refractometer pH 3.5 pH meter salt 20.90% potentiometric titration General bacterial count Below 10 cfu/g Standard agar medium method coliform bacteria feminine BGLB method heat-resistant bacteria Below 10 cfu/g Standard agar medium method (100°C for 10 minutes) Mold / Yeast Below 10 cfu/g potato dextrose agar method [Example 3] Trial production of frozen spring rolls According to the following table and the following procedures, the effect of adding the sterilized clarified salt-added lactic acid bacteria/yeast whey fermented liquid (Invention 1) to frozen spring rolls was confirmed. [table 5] Control Blank Test 1 Test 2 Trial 3 Test 4 Test 5 Test 6 Addition ratio of whey fermented liquid 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% Ingredients Pork tenderloin 12.00% 12.00% 12.00% 12.00% 12.00% 12.00% 12.00% 12.00% cabbage 22.00% 22.00% 22.00% 22.00% 22.00% 22.00% 22.00% 22.00% bamboo shoots 20.00% 20.00% 20.00% 20.00% 20.00% 20.00% 20.00% 20.00% onion 4.00% 4.00% 4.00% 4.00% 4.00% 4.00% 4.00% 4.00% dried shiitake mushrooms 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% Granular vegetable protein 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% lard 3.00% 3.00% 3.00% 3.00% 3.00% 3.00% 3.00% 3.00% Ginger puree 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% seasoning fine white sugar 0.67% 0.67% 0.67% 0.67% 0.67% 0.67% 0.67% 0.67% Sodium Glutamate 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% oyster sauce 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% soy sauce 0.40% 0.40% 0.40% 0.40% 0.40% 0.40% 0.40% 0.40% salt 0.40% 0.40% 0.39% 0.38% 0.36% 0.30% 0.20% 0.00% Japanese sake 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% Pork extract 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% sesame oil 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% starch 2.67% 2.67% 2.67% 2.67% 2.67% 2.67% 2.67% 2.67% Invention 1 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% water 25.2% 25.2% 25.2% 25.1% 25.0% 24.8% 24.4% 23.6% Total 100% 100% 100% 100% 100% 100% 100% 100% Freezing none have have have have have have have ·program (1) Seasoning raw materials other than sesame oil among the raw materials in Table 5 were mixed in advance. (2) Add 1 tablespoon of oil to the preheated frying pan and lightly fry the meat. (3) Add other ingredients and fry. (4) After the ingredients are overheated, the seasoning mixture is added and heated. (5) Turn off the heat after it becomes sticky, add sesame oil and put it on the pad. (6) Wrap 30 g of ingredients in a commercially available spring roll skin. (7) Deep fry in oil at 170°C for 2 minutes. (8) After cooling at room temperature, store in a freezer at -25°C for 2 weeks. Only the control (Control) product was separately used for the evaluation test, and prepared at the same time as the blank (Blank) or the frozen spring rolls of the tests 1 to 6 were heated and thawed, and the frozen spring rolls of the Blank or the tests 1 to 6 were stored as unfrozen. The state is used for evaluation test. ・Evaluation method After being stored in a freezer for 2 weeks, each spring roll test piece was thawed in a microwave oven, and 14 fully trained sensory inspectors set the control (non-frozen product) to 10 points for the goodness of the texture and flavor, and set the Blank ( No added frozen product) was set as 5 points, and the sensory evaluation was performed on a 10-level evaluation, and the average value of the evaluation points of each sensory inspector was obtained. In addition, when rounding off the significant figures of the one digit to the nearest 7 points, it gives a sufficient texture or flavor that the previous product did not have, and it is defined as qualified (the same in the following embodiments). ). ・Evaluation criteria (feel of texture) 1 point: There is no sense of material at all. 2 points: far worse than Blank. 3 points: worse than Blank. 4 points: slightly worse than Blank. 5 points: Same as Blank (no added frozen product). 6 points: Slight improvement over Blank. 7 points: Compared with Blank, 1-2 kinds of ingredients have a sense of material. 8 points: Compared with Blank, more than 3 kinds of ingredients have a sense of material. 9 points: The sense of material is sufficient. Slightly worse than Control. 10 points: It is the same as Control (non-frozen product), or has a superior material feel. ・Evaluation criteria (goodness of flavor) 1 point: The flavor is very poor. 2 points: far worse than Blank. 3 points: worse than Blank. 4 points: slightly worse than Blank. 5 points: Same as Blank (no added frozen product). 6 points: Slight improvement over Blank. 7 points: an improvement over Blank. 8 points: Compared with Blank, it has a similar flavor to spring rolls. 9 points: The spring roll-like flavor is sufficient. Slightly worse than Control. 10 points: Same as Control (non-frozen product), or has a superior flavor. ·Evaluation results The results are shown in FIGS. 1 and 2 . The texture of spring roll ingredients increases with the addition of Invention Product 1, and the effective addition amount is more than 0.1%. The goodness of the overall flavor of the spring rolls is also improved with the increase of the addition amount of the invention product 1, but if the addition amount is 2%, the acidity or aroma of the whey stock solution can be slightly felt, and the similar spring roll food is lost. sense of inclination. Therefore, in the spring rolls of this embodiment, the practically effective addition amount of the invention product 1 can be described as 0.1% to 2.0%. [Example 4] Frozen Spring Roll Trial (Invention 2) According to the following table and the following procedure, the effect of adding the sterilized clarified salt-added lactic acid bacteria-yeast soymilk fermented liquid (Invention 2) to frozen spring rolls was confirmed. [Table 6] Control Blank Test 1 Test 2 Trial 3 Test 4 Test 5 Test 6 Adding ratio of soymilk fermented liquid 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% Ingredients Pork tenderloin 12.00% 12.00% 12.00% 12.00% 12.00% 12.00% 12.00% 12.00% cabbage 22.00% 22.00% 22.00% 22.00% 22.00% 22.00% 22.00% 22.00% bamboo shoots 20.00% 20.00% 20.00% 20.00% 20.00% 20.00% 20.00% 20.00% onion 4.00% 4.00% 4.00% 4.00% 4.00% 4.00% 4.00% 4.00% dried shiitake mushrooms 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% Granular vegetable protein 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% lard 3.00% 3.00% 3.00% 3.00% 3.00% 3.00% 3.00% 3.00% Ginger puree 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% seasoning fine white sugar 0.67% 0.67% 0.67% 0.67% 0.67% 0.67% 0.67% 0.67% Sodium Glutamate 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% 0.33% oyster sauce 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% soy sauce 0.40% 0.40% 0.40% 0.40% 0.40% 0.40% 0.40% 0.40% salt 0.40% 0.40% 0.39% 0.38% 0.36% 0.30% 0.20% 0.00% Japanese sake 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% Pork extract 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% 2.00% sesame oil 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% starch 2.67% 2.67% 2.67% 2.67% 2.67% 2.67% 2.67% 2.67% Invention 2 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% water 25.2% 25.2% 25.2% 25.1% 25.0% 24.8% 24.4% 23.6% Total 100% 100% 100% 100% 100% 100% 100% 100% Freezing none have have have have have have have ·program Frozen foods were prepared by conditioning in the same procedure as in Example 3. ・Evaluation method and evaluation criteria It was carried out in the same manner as in Example 3. ·Evaluation results The results are shown in FIGS. 3 and 4 . The texture of the spring roll ingredients increases with the increase of the addition amount of the invention product 2, and the effective addition amount is the same as that of the invention product 1, which is 0.1% or more. The goodness of the overall flavor of the spring rolls is also improved with the increase of the addition amount of the invention product 2. However, if the addition amount is 2%, the sourness or aroma of the soymilk fermented liquid can be slightly felt, and the similar spring roll food is lost. sense of inclination. Therefore, in this embodiment, the practically effective addition amount of the invention product 2 can be described as 0.1% to 2.0%. [Example 5] Trial preparation of ingredients for Chinese rice bowl (Invention 1) According to the following table and the following procedure, the effect of adding the sterilized clarified salt-added lactic acid bacteria/yeast whey fermented liquid (Invention 1) to the ingredients of frozen Chinese rice bowl was confirmed. [Table 7] Control Blank Test 1 Test 2 Trial 3 Test 4 Test 5 Test 6 Invention product addition ratio 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% Ingredients shrimp 3.0% 3.0% 3.0% 3.0% 3.0% 3.0% 3.0% 3.0% squid 4.0% 4.0% 4.0% 4.0% 4.0% 4.0% 4.0% 4.0% carrot 3.0% 3.0% 3.0% 3.0% 3.0% 3.0% 3.0% 3.0% Chinese cabbage 15.0% 15.0% 15.0% 15.0% 15.0% 15.0% 15.0% 15.0% bamboo shoots 4.0% 4.0% 4.0% 4.0% 4.0% 4.0% 4.0% 4.0% fungus 1.5% 1.5% 1.5% 1.5% 1.5% 1.5% 1.5% 1.5% pod 0.5% 0.5% 0.5% 0.5% 0.5% 0.5% 0.5% 0.5% pork 3.5% 3.5% 3.5% 3.5% 3.5% 3.5% 3.5% 3.5% sauce minced green onion 0.31% 0.31% 0.31% 0.31% 0.31% 0.31% 0.31% 0.31% Ginger puree 0.17% 0.17% 0.17% 0.17% 0.17% 0.17% 0.17% 0.17% Japanese sake 2.17% 2.17% 2.17% 2.17% 2.17% 2.17% 2.17% 2.17% salt 0.40% 0.40% 0.39% 0.38% 0.36% 0.30% 0.20% 0.00% granulated sugar 1.13% 1.13% 1.13% 1.13% 1.13% 1.13% 1.13% 1.13% Sodium Glutamate 0.31% 0.31% 0.31% 0.31% 0.31% 0.31% 0.31% 0.31% black pepper 0.06% 0.06% 0.06% 0.06% 0.06% 0.06% 0.06% 0.06% Chicken Noodle Soup 1.21% 1.21% 1.21% 1.21% 1.21% 1.21% 1.21% 1.21% seafood essence 2.06% 2.06% 2.06% 2.06% 2.06% 2.06% 2.06% 2.06% Chinese broth powder 0.52% 0.52% 0.52% 0.52% 0.52% 0.52% 0.52% 0.52% soy sauce 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% 1.00% starch 2.60% 2.60% 2.60% 2.60% 2.60% 2.60% 2.60% 2.60% Invention 1 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% water 52.94% 52.94% 52.90% 52.86% 52.78% 52.54% 52.14% 51.34% sesame oil 0.62% 0.62% 0.62% 0.62% 0.62% 0.62% 0.62% 0.62% total 100.0% 100.0% 100.0% 100.0% 100.0% 100.0% 100.0% 100.0% Freezing none have have have have have have have ·program (1) A part of the sauce raw materials in the table (from Japanese sake to the invention product 1) were mixed in advance. (2) The ingredients are blanched one by one and placed on the pad. (3) Put chopped green onion and ginger puree into the pot. (4) Throwing in (1) and heating. (5) Turn off the heat after it becomes sticky. (6) Put in sesame oil and mix. (7) Throw in the blanched ingredients and mix. (8) After cooling at room temperature, store in a freezer at -25°C for 2 weeks. Only the Control product was separately used for the evaluation test, and it was prepared at the same time as the ingredients of Blank or the Chinese rice bowl of Tests 1 to 6 were heated and thawed. The state is used for evaluation test. ・Evaluation method After being stored in the freezer for 2 weeks, the ingredients of each Chinese rice bowl were thawed in a microwave oven. 14 fully trained sensory inspectors set the Control (non-frozen product) design according to the quality of the texture, texture and flavor. As 10 points, Blank (frozen product without addition) was set as 5 points, and the sensory evaluation was performed on a 10-level evaluation, and the average value of the evaluation points of each sensory inspector was calculated. ・Evaluation criteria (feel of texture) 1 point: There is no sense of material at all. 2 points: far worse than Blank. 3 points: worse than Blank. 4 points: slightly worse than Blank. 5 points: Same as Blank (no added frozen product). 6 points: Slight improvement over Blank. 7 points: Compared with Blank, 1 to 3 ingredients have a sense of material. 8 points: Compared with Blank, more than 4 kinds of ingredients have a sense of material. 9 points: The sense of material is sufficient. Slightly worse than Control. 10 points: It is the same as Control (non-frozen product), or has a superior material feel. ・Evaluation criteria (goodness of flavor) 1 point: The flavor is very poor. 2 points: far worse than Blank. 3 points: worse than Blank. 4 points: slightly worse than Blank. 5 points: Same as Blank (no added frozen product). 6 points: Slight improvement over Blank. 7 points: an improvement over Blank. 8 points: Compared with Blank, it has a flavor similar to the ingredients of Chinese rice bowl. 9 points: The flavor of the ingredients similar to the Chinese rice bowl is sufficient. Slightly worse than Control. 10 points: Same as Control (non-frozen product), or has a superior flavor. ·Evaluation results The results are shown in FIGS. 5 and 6 . [Example 6] Trial preparation of ingredients for Chinese rice bowl (Invention 2) In the table of Example 5, except that the invention product 1 was changed to the invention product 2, it was confirmed that the clarification of the salt-added lactic acid bacteria-yeast soybean milk fermentation liquid (the invention product 2) was changed according to the following procedures. Added effect of frozen spring rolls. ·program Frozen foods were prepared by conditioning using the same procedure as in Example 5. ・Evaluation method and evaluation criteria It was carried out in the same manner as in Example 5. ·Evaluation results The results are shown in FIGS. 7 and 8 and the following table. In addition, the food tasting comments of the sensory inspectors are shown in the following table. [Table 8] Control Blank Test 1 Test 2 Trial 3 Test 4 Test 5 Test 6 add ratio 0.00% 0.00% 0.05% 0.10% 0.20% 0.50% 1.00% 2.00% Comments ・Ordinary taste ・I feel a little lacking ・It is difficult to distinguish the flavor of the ingredients ・No big difference compared to Blank ・Strongly feel the flavor of the ingredients ・Easy to distinguish the taste of cabbage and fungus ・It is not a non-frozen product, but a strong sense of food is felt. ・Slightly strong salty taste ・Feel the texture and flavor of the ingredients close to non-frozen products ・Feel the texture and flavor of food close to non-frozen products ・The flavor of the fermented liquid is strong, and it is slightly inconsistent. ・The salty taste is strong Depending on the food, the effect of promoting the salty taste can be seen. [Example 7] Preparation of fermented liquid using different strains A fermented liquid was prepared according to Example 1 or 2, except that other lactic acid bacteria or yeast were used. [Table 9] Example 1 Example 2 Example 7 Example 8 Example 9 Example 10 raw material whey soy milk soy milk whey soy milk soy milk Lactic acid bacteria Lactobacillus strain YU2 Lactobacillus rhamnosus AN1 strain Lactococcus lactis Lactobacillus acidophilus Fusion Lactobacillus Lactobacillus badensis yeast Saccharomyces cerevisiae FAHP-2 Saccharomyces cerevisiae (white wine) FAHP-1 Saccharomyces cerevisiae Schizosaccharomyces pombe Saccharomyces cerevisiae Saccharomyces cerevisiae (sake) Comments on modulation The 3-liter small culture tank test culture conditions were as described in Example 1 The 3-liter small culture tank test culture conditions were as described in Example 1 Flask culture test. The basic conditions are the same as those in Examples 1 and 2 Flask culture test. The basic conditions are the same as those in Examples 1 and 2 Flask culture test. The basic conditions are the same as those in Examples 1 and 2 Flask culture test. The basic conditions are the same as those in Examples 1 and 2 Comments on the fermented liquid Strong ester and fruit aroma Strong sweet flavor The same fermented liquid as in Example 1 was obtained, although it was different in that the flavor of the cheese was strong. Although the odor of ethyl acetate was strong, the same fermented liquid as in Example 1 was obtained. The same fermented liquid as in Example 1 was obtained, although it was different in that the sweet aroma of condensed milk was strong. Although the yoghurt flavor and the fruit flavor were strong, the same fermented liquid as in Example 1 was obtained. The fermented liquids obtained in Examples 7 to 10 are all those that can expect the desired effect. [Example 8] Differentiation test of ingredients According to the following procedure, prepare a liquid for separating the components contained in the clarified salt-added lactic acid bacteria/yeast whey fermented liquid (inventive product 1). ·program (1) Concentrate the clarified salt-added lactic acid bacteria/yeast whey fermented liquor to two times by concentration under reduced pressure. (2) A dialysis tube (Spectra/Por, Funakoshi) having a molecular weight of 20,000 was filled with (1), and dialyzed against distilled water while stirring (4° C., 24 hours, distilled water 1 L×3 exchanges). (3) The sample in the dialysis tube is set as the distinguishing solution 1. (4) The distilled water side was concentrated under reduced pressure so as to be the same amount as before dialysis. (5) A dialysis tube (Spectra/Por, Funakoshi) having a molecular weight of 3,500 to 5,000 was filled with (4), and dialysis was performed in distilled water while stirring (4°C, 24 hours, 1 L of distilled water × 3 exchanges). (6) The sample in the dialysis tube is set as the distinguishing solution 2. (7) The distilled water side was concentrated under reduced pressure so as to be the same amount as before dialysis. (8) A dialysis tube (Spectra/Por, Funakoshi) having a molecular weight of 100 to 500 was filled with (7), and dialyzed against distilled water while stirring (4°C, 24 hours, 1 L of distilled water × 3 exchanges). (9) The sample in the dialysis tube is set as the distinguishing solution 3. (10) The distilled water side was concentrated under reduced pressure so as to be the same amount as before dialysis, and it was set as the fractional solution 4 . ・Details of different liquids The molecular weights of the components contained in the respective fractions are shown in the following table. [Table 10] Differentiate molecular weight Division 1 Molecular weight>20000 Division 2 (3500~)5000<Molecular weight<20000 Division 3 (100~)500<Molecular weight<3500(~5000) Division 4 Molecular weight<100(~500) ・Evaluation results of each liquid By the same method as in Example 3, the effect of adding the clarified salt-added lactic acid bacteria/yeast whey ferment liquid to frozen spring rolls was confirmed. [Table 11] Material Sense Evaluation Control Invention 1 2x Concentrate 8 Division 1 Molecular weight>20000 components 5 Division 2 (3500~)5000<Molecular weight<20000 components 6 Division 3 (100~)500<Molecular weight<3500(~5000)component 8 Division 4 Molecular weight <100 (~500) components 6 The results are shown in the above table. The texture of the spring roll ingredients was strongly felt as the fractional molecular weight became smaller, and in the zone where the fractional solution 3 was added, a texture equivalent to that of the invention 1 was felt. Therefore, in the conditions of this Example, the active ingredient of the invention product 1 is considered to be a component of molecular weight (100~) 500~3500 (~5000). [Example 9] Roasted tender chicken nuggets trial (Invention 2) According to the table below and the following procedures, confirm the effect of adding sterilized clarified salt-added lactic acid bacteria and soybean milk fermented liquid (Invention 2) to fragrant roasted tender chicken nuggets. [Table 12] Blank Test 1 Invention 2 0.00% 0.33% Sodium Bicarbonate (Preparation) 0.37% 0.00% chicken breast 74.07% 74.10% salt 0.81% 0.82% fine white sugar 3.19% 3.19% Liquid sugar 0.74% 0.74% soy sauce 2.22% 2.22% sake 2.00% 2.00% Chicken essence 0.22% 0.22% vegetable oil 0.22% 0.22% processed starch 2.07% 2.07% water 14.07% 14.08% Total 100.00% 100.00% ·program (1) Mix the seasoning liquid raw materials (raw materials other than chicken breast meat) described in the preparation table. (2) The chicken breast meat is cut, mixed with the seasoning liquid obtained in (1), and immersed. (3) The chicken breast meat obtained is heated in an oven. (4) Quickly freeze the heated chicken breast meat. ・Evaluation method After being stored in a freezer for 3 days, thawed in a microwave oven, and cooled to room temperature. 10 fully trained sensory inspectors rated Blank (no additives) as 5 points for the softness and juiciness of the food texture. The sensory evaluation was performed on 10-level evaluation, and the average value of the evaluation points of each sensory examiner was obtained. ・Evaluation criteria (common to each evaluation item) 1 point: Very worse than Blank. 2 points: far worse than Blank. 3 points: worse than Blank. 4 points: slightly worse than Blank. 5 points: Same as Blank (no additives). 6 points: a little higher than Blank. 7 points: Slight improvement over Blank. 8 points: an improvement over Blank. 9 points: a sufficient improvement over Blank. 10 points: Great improvement over Blank. ·Evaluation results The results are shown in FIG. 9 . Inventive product 2 additive strain was evaluated as follows: it was softer and more juicy than sodium bicarbonate preparation additive. ・The chicken breast meat obtained in the above procedure was stored in a freezer for 3 days, thawed in a microwave oven, cooled to room temperature, and then cooled to room temperature by a Texture analyzer (manufactured by Stable Micro Systems), using Yamaden Wedge Plunger No. .49, the crushing test is carried out under the conditions of a speed of 1.0 mm/sec and a deformation rate of 100%. The results are shown in FIG. 10 . Compared with the additive product of sodium bicarbonate preparation, the additive product 2 of the invention is broken with a weaker force. [Example 10] Unadjusted Soymilk Addition Test (Invention 2) According to the following table and the following procedures, the effect of adding sterilized clarified salt-added lactic acid bacteria-soymilk fermentation liquid (Invention 2) to unadjusted soymilk was confirmed. Furthermore, the test was carried out within the range of 0.4% to 1% of the addition amount, which was known to obtain a higher effect from the results of the previous test. [Table 13] Blank Test 1 Test 2 Trial 3 Test 4 Test 5 Test 6 Test 7 Invention 2 0.00% 0.40% 0.50% 0.60% 0.70% 0.80% 0.90% 1.00% Unadjusted Soymilk (Ji Wen) 99.80% 99.48% 99.40% 99.32% 99.24% 99.16% 99.08% 99.00% salt 0.20% 0.12% 0.10% 0.08% 0.06% 0.04% 0.02% 0.00% Total 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% 100.00% ·program Inventive product 2 (0, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1%) was added to unadjusted soymilk (Kiwen), and the salt was supplemented with table salt. ・Evaluation method 10 fully trained sensory examiners evaluated the sweetness, smoothness, thickness of taste, and masking of fishy smell, and scored Blank (no additives) as 5 points, and performed sensory evaluation on a 10-level evaluation, and obtained the results. The average of the evaluation points of each sensory examiner. ・Evaluation criteria (common to each evaluation item) 1 point: very weak. 2 points: far weaker than Blank. 3 points: weaker than Blank. 4 points: slightly weaker than Blank. 5 points: Same as Blank (no additives). 6 points: slightly stronger than Blank. 7 points: slightly stronger than Blank. 8 points: Better than Blank. 9 points: substantially stronger than Blank. 10 points: very strong than Blank. ·Evaluation results The results are shown in FIG. 11 . Inventive product 2 with an additive system was evaluated as follows: sweetness, smoothness, and thickness of taste were increased, and the fishy smell of soymilk was masked compared with the non-additive product. All items increased proportionally until the addition rate was 0.7%, after which the increase became slow. Moreover, when the addition rate is 1.0%, an unpleasant taste or astringency will appear. [Example 11] Test of adding hamburger patty (Invention 2) According to the following table and the following procedures, the vegetable protein of the sterilized clarified salt-added lactic acid bacteria and soymilk yeast solution (invention product 2) was confirmed in the hamburger patty in which 40% of the minced meat was changed to vegetable protein (soy protein). Odor masking effect. In addition, the junmai cooking wine which was previously used as a comparison object for the odor masking of vegetable protein was added so that it might become the same manufacturing cost. [Table 14] Blank Test 1 Test 2 Deployment A Beef and pork mince mix (7:3) 40.94% 40.94% 40.94% salt 0.45% 0.45% 0.45% Fried onion ※2 13.65% 13.65% 13.65% raw bread flour 6.82% 6.82% 6.82% whole egg 4.78% 4.78% 4.78% milk 4.78% 4.78% 4.78% tomato juice 1.02% 1.02% 1.02% Nutmeg Powder 0.10% 0.10% 0.10% black pepper (powder) 0.10% 0.10% 0.10% garlic (powder) 0.07% 0.07% 0.07% Vegetable protein + seasoning liquid Granular vegetable protein*1 6.82% 6.82% 6.82% water 20.15% 17.76% 18.88% pure rice cooking wine 2.44% Invention 2 1.59% salt 0.32% 0.26% 0.00% Total 100.00% 100.00% 100.00% *1 "New Fujinic 41SH" manufactured by Fuji Oil Co., Ltd. *2 To fry onions: Heat 30 g of unsalted butter and 30 g of salad oil in a frying pan, fry 500 g of onions and 2 pieces of bay bark, cool them down, and use them. ·program (1) The vegetable protein was immersed in the seasoning liquid for 30 minutes. (2) Mix and prepare the ingredients of A, and stir until it becomes sticky. (3) The immersed vegetable protein was added to the preparation A, followed by stirring. (4) Ferment for 30 minutes in the refrigerator. (5) After dividing into 120 g each, molding, and heating in an oven at 230° C. for 10 minutes. (6) After the rapid freezing, vacuum packaging is performed and frozen storage is performed. (7) Heating with hot water when tasting food. ・Evaluation method 10 fully trained sensory inspectors performed sensory evaluations on a 10-level evaluation scale with respect to plant protein odor masking, softness, juiciness, and meatiness, and scored Blank (no additives) as 5 points, and obtained each sensory evaluation. The average of the inspectors' evaluation points. ・Evaluation criteria (common to each evaluation item) 1 point: Very worse than Blank. 2 points: far worse than Blank. 3 points: worse than Blank. 4 points: slightly worse than Blank. 5 points: Same as Blank (no additives). 6 points: a little improvement over Blank. 7 points: Slight improvement over Blank. 8 points: an improvement over Blank. 9 points: a sufficient improvement over Blank. 10 points: Great improvement over Blank. ·Evaluation results The results are shown in FIG. 12 . Compared with the additive-free product or Junmai cooking wine, the invention product 2 has improved all the evaluation items. In particular, vegetable protein has a higher odor masking effect. Also, cooking sake is given a unique flavor of sake. However, the soymilk fermented liquid-added strain was only given various effects without changing the flavor itself. [Example 12] Japanese pancake sauce addition test (Invention 2) According to the table below and the following procedures, the effect of adding sterilized clarified salt-added lactic acid bacteria and soybean milk yeast solution (Invention 2) to Japanese pancake sauce was confirmed. [Table 15] Control Test 1 Japanese pancake sauce Invention 2 0.00% 0.20% sugar (liquid sugar) 35.00% 35.00% Sugar (granulated sugar) 4.60% 4.60% Vegetables/Fruits (Tomatoes) 8.00% 8.00% applesauce 2.80% 2.80% apple juice concentrate 2.40% 2.40% Vegetables/Fruits (Onions) 0.20% 0.20% Vegetables/Fruits (Garlic) 0.20% 0.20% protein hydrolyzate 8.86% 8.86% brewing vinegar 5.09% 5.09% salt 1.00% 1.00% Spices ※1 0.20% 0.20% Tackifier 2.50% 2.50% pigment 1.00% 1.00% chemical seasoning 0.46% 0.46% raw water 27.69% 27.49% Total 100.00% 100.00% *1 Use a blend of black pepper, chilli, cinnamon, nutmeg, cloves, sage, thyme, bay, and dried tangerine peel. ·program (1) Soymilk fermented liquid (Invention 2) was added to the Japanese pancake sauce prepared with the ingredients listed in the table. ・Evaluation method 10 fully trained sensory inspectors performed sensory evaluations on a 10-level evaluation scale with regard to spice, sourness, ripeness, and richness. The average of the rating points. ・Evaluation criteria (common to each evaluation item) 1 point: Very worse than Blank. 2 points: far worse than Blank. 3 points: worse than Blank. 4 points: slightly worse than Blank. 5 points: Same as Blank (no additives). 6 points: a little improvement over Blank. 7 points: Slight improvement over Blank. 8 points: an improvement over Blank. 9 points: a sufficient improvement over Blank. 10 points: Great improvement over Blank. ·Evaluation results The results are shown in FIG. 13 . Compared with the additive-free product, the additive product of the invention product 2 has improved spice, maturity, and richness. In particular, the sense of spice (stimulation) is significantly improved. Also, it has the effect of causing an aftertaste. [Example 13] Hot chocolate prototype test (inventive product 2) According to the following table and the following procedures, the effect of adding the sterilized clarified salt-added lactic acid bacteria and soybean milk fermented liquid (Invention 2) to hot chocolate was confirmed. [Table 16] Blank Test 1 cocoa pulp 10.84% 10.84% granulated sugar 10.84% 10.84% Unadjusted soymilk 77.47% 77.47% Invention 2 0.00% 0.85% salt water 0.85% 0.00% total 100.00% 100.00% ·program (1) The cocoa slurry is melted with hot water. (2) Unadjusted soymilk was heated to 50° C., about half of it was poured into the melted cocoa liquor, and mixed. (3) The remaining soybean milk and sugar were also added and mixed. (4) Mixed salt water or invention product 2. ・Evaluation method After the trial production, for the samples set at 10°C, 20°C, and 40°C, four fully trained sensory inspectors performed the sensory evaluation on a 10-level evaluation with Blank (no additives) as 5 points for the cocoa flavor. , and obtain the average value of the evaluation points of each sensory inspector. ・Evaluation criteria 1 point: very weak. 2 points: far weaker than Blank. 3 points: weaker than Blank. 4 points: slightly weaker than Blank. 5 points: Same as Blank (no additives). 6 points: slightly stronger than Blank. 7 points: slightly stronger than Blank. 8 points: Better than Blank. 9 points: substantially stronger than Blank. 10 points: very strong than Blank. ·Evaluation results The results are shown in FIG. 14 . Inventive product 2 additive product (test 1) was evaluated as follows: Compared with Blank, the cocoa flavor was improved. Also, the higher the temperature, the greater the effect of improving the cacao feel. [Example 14] Cream Chocolate Addition Test (Invention 2) According to the following table and the following procedures, confirm the effect of adding sterilized clarified lactic acid bacteria and soybean milk fermented liquid (Invention 2, but without salt) to cream chocolate. [Table 17] Blank Test 1 Slab Cream Chocolate 99.0% 99.0% water 1.0% 0.0% Invention 2 (no salt) 0.0% 1.0% total 100.0% 100.0% ·program (1) Melt the cream chocolate with hot water. (2) Mixed water or invention product 2. ・Evaluation method After the trial production, for the samples that were cooled to become solid and the samples to be liquid at 40°C, six fully trained sensory inspectors rated the cacao flavor as 5 points for Blank (no additives), and evaluated them on a 10-point scale. The evaluation was carried out, and the average value of the evaluation points of each sensory inspector was obtained. ・Evaluation criteria 1 point: very weak. 2 points: far weaker than Blank. 3 points: weaker than Blank. 4 points: slightly weaker than Blank. 5 points: Same as Blank (no additives). 6 points: slightly stronger than Blank. 7 points: slightly stronger than Blank. 8 points: Better than Blank. 9 points: substantially stronger than Blank. 10 points: very strong than Blank. ·Evaluation results The results are shown in FIG. 15 . Inventive product 2 additive product (test 1) was evaluated as follows: Compared with Blank, the cocoa flavor was improved. In addition, the cocoa flavor enhancement effect of the liquid is greater. [Example 15] Light-irradiated milk and soybean milk addition test (Invention 2) According to the following table and the following procedures, the effect of adding the sterilized clarified salt-added lactic acid bacteria and soybean milk fermentation liquid (Invention 2) to light-irradiated milk and light-irradiated soymilk was confirmed. [Table 18] Blank Test 1 milk 99.5% 99.5% Invention 2 0.0% 0.5% salt water 0.5% 0.0% total 100.0% 100.0% Blank Test 1 Unadjusted soymilk 99.0% 99.0% Invention 2 0.0% 1.0% salt water 1.0% 0.0% total 100.0% 100.0% ·program (1) Mix salt water or invention product 2 with milk or unadjusted soymilk. (2) Light irradiation (5°C, 26000 lux, 5 days) with an incubator FLI-2010T with illumination (EYELA Tokyo RIKEN CO., LTD.) ・Evaluation method For the samples after 5 days, two fully trained sensory inspectors evaluated the effect of suppressing light deterioration odor. The Blank that was irradiated with light was rated as 1 point, and the sample that was not irradiated with light was rated as 10 points, and evaluated on a 10-level scale. The sensory evaluation was performed, and the average value of the evaluation points of each sensory examiner was obtained. ・Evaluation criteria 1 point: The effect of suppressing the light-deterioration odor is very weak (the light-deterioration odor is very strong compared with the Blank which is not irradiated with light, but is equivalent to the Blank which is irradiated with light). 2 points: The effect of suppressing light-deterioration odor is weak (the light-deterioration odor is much stronger than the Blank without light irradiation, but weaker than the Blank with light irradiation) 3 points: The light-deterioration odor suppression effect is weak (the light-deterioration odor is very strong compared to the Blank which is not irradiated with light, but slightly weaker than the Blank which is irradiated with light). 4 points: The light-deterioration odor suppression effect is slightly weak (the light-deterioration odor is very strong compared to the Blank that is not irradiated with light, but weaker than the Blank that is irradiated with light). 5 points: The light-deterioration odor suppression effect is weak (the light-deterioration odor is very strong compared with Blank without light irradiation, but far weaker than Blank with light irradiation). 6 points: The light-deterioration odor suppression effect is slightly strong (the light-deterioration odor is far stronger than Blank without light irradiation). 7 points: The effect of suppressing light-deterioration odor is slightly stronger (light-deterioration odor is stronger than Blank without light irradiation). 8 points: The effect of suppressing the light-deterioration odor is strong (the light-deterioration odor is slightly stronger than that of Blank which was not irradiated with light). 9 points: The effect of suppressing the light deterioration odor is very strong (the light deterioration odor is slightly stronger than that of Blank which is not irradiated with light). 10 points: The effect of suppressing light deterioration odor is very strong (the light deterioration odor is the same as Blank (complete) without light irradiation). ·Evaluation results The results are shown in FIGS. 16 and 17 . Both cow's milk and soymilk produce a deteriorating odor due to light irradiation, but the additive product (Test 1) of the invention product 2 was evaluated as follows: compared with Blank, the photo-deteriorating odor was suppressed. [Example 16] Cream Prototype Test (Invention 2) As shown in the table below, the effect of adding sterilized clarified salt-added lactic acid bacteria-soybean milk fermented liquid (Invention 2) to cream was confirmed. [Table 19] Blank Test 1 oil layer vegetable oil 29.7% 29.7% Lecithin 1.5% 1.5% water layer milk 58.4% 58.4% fatty acid glycerides 0.2% 0.2% sodium caseinate 0.1% 0.1% sodium metaphosphate 0.1% 0.1% Invention 2 0.0% 0.1% Milk fat raw material whipped cream 10.0% 9.9% total 100.0% 100.0% ·program (1) Oil layer The oil is heated, and when the oil is dissolved, lecithin and an emulsifier are added and mixed. (2) Water layer Moisture is heated, and materials are added and mixed. (3) The water layer and the oil layer are mixed and pre-emulsified, and thereafter, the homogenization treatment is performed and cooling is performed. ・Evaluation method Sensory evaluation of the milk flavor of the obtained samples was carried out by 2 well-trained sensory inspectors. ·Evaluation results Inventive product 2 added strains were evaluated as follows: Compared with Blank, the milk flavor was improved. [Example 17] Trial test of creamy beef hash browns (Invention 2) As shown in the table below, the effect of adding the sterilized clarified salt-added lactic acid bacteria-soymilk yeast solution (Invention 2) to the ingredients in the creamy beef hash browns was confirmed. [Table 20] Raw material name Blank Test 1 wheat flour 5.00% 5.00% processed starch 3.00% 3.00% salt 0.20% 0.20% crab slices 5.00% 5.00% fine white sugar 1.20% 1.20% clear soup powder 0.50% 0.50% water 9.10% 9.00% milk 30.00% 30.00% whipped cream 5.00% 5.00% margarine 1.50% 1.50% sourdough butter 0.10% 0.10% onion 30.00% 30.00% salad oil 2.20% 2.20% Processing aids 4.20% 4.20% seafood essence 2.00% 2.00% Invention 2 0.00% 0.10% yolk 1.00% 1.00% total 100.00% 100.00% ·program (1) After frying the onion, the raw materials described in the table are mixed and heated. (2) cooling the ingredients obtained in (1), making it solidify, molding and coating, (3) (2) was deep-fried (180 degreeC 3 minutes), and the croquette was obtained. ・Evaluation method Sensory evaluation of the milk flavor of the obtained samples was carried out by 2 well-trained sensory inspectors. ·Evaluation results Inventive product 2 additive product (Test 1) was evaluated as follows: Compared with Blank, the milk flavor was improved. [Example 18] Addition test to instant miso juice (Invention 2) According to the following table and the following procedures, the effect of adding sterilized clarified salt-added lactic acid bacteria and soybean milk fermented liquid (Invention 2) to instant miso juice was confirmed. As seasoning miso for instant miso juice, there are seasoned miso (“Asage” made by Naganien, using soybean miso), red miso (“Hiruge” by Naganien, using soybean miso), white miso (“Nagaenen” “Hiruge” Yu-Ge" original miso type, using soybean miso). [Table 21] Raw material name Blank Test 1 seasoned miso 9.25% 9.25% salt water 0.20% 0.00% Invention 2 0.00% 0.20% hot water 90.56% 90.56% total 100.00% 100.00% ·program (1) Seasoning miso, salt water, and invention product 2 are mixed. (2) Hot water is injected and mixed. ・Evaluation method After trial production, for samples cooled to room temperature, four fully trained sensory inspectors set Blank (no additives) as 5 points for the intensity of grain flavor (soybean flavor), sweetness, and umami. 10-level evaluation The sensory evaluation was performed, and the average value of the evaluation points of each sensory examiner was obtained. ・Evaluation criteria 1 point: Very weak. 2 points: Far weaker than Blank. 3 points: weaker than Blank. 4 points: slightly weaker than Blank. 5 points: Same as Blank (no additives). 6 points: slightly stronger than Blank. 7 points: slightly stronger than Blank. 8 points: Better than Blank. 9 points: substantially stronger than Blank. 10 points: very strong than Blank. ·Evaluation results The results are shown in FIG. 18 . Inventive product 2 additive (test 1) was evaluated as follows: cereal flavor (soybean flavor), sweetness, and umami were improved compared to Blank. Its effect is especially great in instant miso juice of white miso and blended miso. In addition, the same effect was confirmed even in the freeze-dried instant miso juice, in addition to the described original miso type. [Example 19] Addition test to salmon floss (Invention 2) According to the following table and the following procedures, the effect of adding sterilized clarified salt-added lactic acid bacteria and soybean milk fermentation liquid (Invention 2) to salmon floss was confirmed. [Table 22] [%] Raw material name Blank Test 1 salmon floss 10.00 10.00 Invention 2 - 0.20 salt 0.04 - hot water 89.96 89.80 total 100.00 100.00 ·program (1) Mix the amounts of hot water, salt water and invention product 2 listed in the table. (2) The liquid mixed in (1) is mixed with salmon floss. ・Evaluation method Regarding the obtained samples, two fully trained sensory inspectors performed sensory evaluations on a 3-level evaluation with Blank as +++ (3 points) for fishy smell and fish odor, and obtained the results of each sensory evaluation. The average of the inspectors' evaluation points. Furthermore, the unpleasant odor of raw fish is called "fishy smell", and the peculiar smell of heated fish is called "fish odor". ·Evaluation results The results are shown in the table. Inventive product 2 added strain was evaluated as follows: compared with Blank, fishy smell or fish odor was suppressed. [Table 23] sample name fishy smell fish smell Evaluation Blank +++ +++ Unique fish odor, fishy smell Test 1 + + The overall fish odor is weakened, and no fishy smell is felt Weak+ <---> +++ Strong [Example 20] Addition test to viscera soup (Invention 2) According to the following table and the following procedures, the effect of adding sterilized clarified salt-added lactic acid bacteria and soybean milk fermented liquid (Invention 2) to the soup of internal organs was confirmed. [Table 24] seasoning liquid [%] Raw material name Blank Test 1 light soy sauce 5.70 5.70 Flavor stock (kelp)* 0.30 0.30 Cooked Kitchen Bonito L* 0.30 0.30 Sodium L-Glutamate 0.10 0.10 Beef extract* 2.00 2.00 Invention 2 - 0.20 salt 0.04 - water 91.56 91.40 total 100.00 100.00 *Made by Fuji Food Industry Co., Ltd. [Table 25] Offal Pot Soup Raw material name Allocation rate [%] Boiled pork offal 20 The above seasoning liquid 80 total 100 ·program (1) The raw materials described in the table were mixed to prepare a seasoning liquid. (2) The seasoning liquid and the boiled pork offal were filled in the bag at the mixing rate shown on the table, heat-sterilized at 90° C. for 10 minutes, water-cooled, and then frozen to prepare viscera soup. (3) After thawing the viscera soup, it was heated together with the packaging to prepare a sample for sensory evaluation. ・Evaluation method With respect to the samples obtained by the above procedure, two fully trained sensory inspectors performed sensory evaluations on a 3-level evaluation with Blank set to +++ (3 points) for visceral odor, and obtained each sensory inspection. The average of the evaluation points of the members. ·Evaluation results The results are shown in the table. Inventive product 2 added strain was evaluated as follows: Compared with Blank, the odor of internal organs was suppressed. [Table 26] sample name visceral smell Evaluation Blank +++ There is an animal odor unique to the internal organs Test 1 + Visceral gas suppressed Weak+ <---> +++ Strong [Example 21] Test of adding ingredients to beef bowl (inventive product 2) According to the following table and the following procedures, the effect of adding sterilized clarified salt-added lactic acid bacteria and soybean milk fermented liquid (Invention 2) to beef bowl (ingredients) was confirmed. [Table 27] Raw Seasoning Liquid for Beef Donburi [%] Raw material name Blank Test 1 thick soy sauce 12.00 12.00 light soy sauce 12.00 12.00 fine white sugar 6.50 6.50 Brewing sweet wine 2.50 2.50 White wine 2.00 2.00 100% orange juice 1.50 1.50 Dried Bonito Chips Extract P* 1.50 1.50 Grain vinegar (4, 2% acidity) 1.00 1.00 Rich Seaweed Dashi* 0.60 0.60 processed starch 0.60 0.60 Ginger Extract 0.10 0.10 water 50.00 50.00 salt 0.30 0.15 Invention 2 - 0.74 make water 9.40 8.81 total 100.00 100.00 *Made by Fuji Food Industry Co., Ltd. [Table 28] Ingredients for Beef Donburi Raw material name Allocation rate [%] Beef (produced in Australia) 40.6 onion 32.4 Zuo Kee Beef Donburi Raw Seasoning Liquid 27.0 total 100.0 ·program (1) The raw materials described in the table were mixed, heated to 85° C. with hot water, and then cooled to prepare a seasoning liquid. (2) After boiling beef and onion, it is cooled. (3) The beef, onion, and seasoning liquid were put into the package at the mixing rate described in the table, and heated at 90° C. for 10 minutes. It is water-cooled and then frozen to make a raw material for beef donburi. (4) After thawing the raw material of the beef bowl of (3), it was heated together with the packaging to prepare a sample for sensory evaluation. ・Evaluation method For the samples obtained by the above procedure, two fully trained sensory inspectors performed sensory evaluations on a 3-level evaluation with Blank set to +++ (3 points) for the grass odor of beef, and obtained each The average of the evaluation scores of the functional examiners. ·Evaluation results The results are shown in the table. Inventive product 2 added strains were evaluated as follows: Compared with Blank, the grassy smell of beef was suppressed. [Table 29] sample name Grass stinks Evaluation Blank +++ Meat-like grass (grass) smell Test 1 + Overall odor disappears weak + <---> +++ strong [Example 22] Addition test to seasoned pork (Invention 2) According to the table below and the following procedures, the effect of adding sterilized clarified salt-added lactic acid bacteria and soybean milk fermented liquid (Invention 2) to seasoned pork was confirmed. [Table 30] [%] Raw material name Blank Test 1 Seasoned minced pork (5 mm) 100 100 Invention 2 - 0.2 total 100 100.2 ·program (1) Using a meat mincer, make 5 mm minced pork in a semi-thawed state of seasoned pork. (2) The other materials described in the table are mixed with the seasoned pork to be minced meat, put in a package, and heated at 90° C. for 10 minutes. After cooling this, a sample for sensory evaluation was prepared. ・Evaluation method For the samples obtained by the above procedure, two fully trained sensory inspectors performed sensory evaluation on a 3-level evaluation with Blank set to +++ (3 points) for the smell of meat in seasoned pork, and obtained The average of the evaluation points of each sensory examiner. ·Evaluation results The results are shown in the table. Inventive product 2 added strain was evaluated as follows: Compared with Blank, the animal odor of pork was suppressed. [Table 31] sample name stinky meat Evaluation Blank +++ Smells like pork beasts Test 1 + The stench of the whole pork beast disappears weak + <---> +++ strong [Example 23] Addition test to tomato sauce (Invention 2) According to the following table and the following procedures, the effect of adding the sterilized clarified salt-added lactic acid bacteria and soybean milk fermentation liquid (Invention 2) to tomato sauce was confirmed. [Table 32] (tomato sauce preparation) raw materials Allocation rate [%] onion (diced) 25.00 whole peeled tomatoes 19.50 tomato puree 13.00 tomato paste 5.20 White wine 3.00 olive oil 3.00 garlic paste 1.00 granulated sugar 1.00 Sanxianjiao 0.10 White pepper 0.10 garlic powder 0.05 Laurel Bark Powder 0.03 salt 0.70 water 28.32 total 100.00 [Table 33] [%] Raw material name Blank Test 1 tomato sauce 99.00 99.00 salt 0.10 0.06 Invention 2 - 0.20 water 0.90 0.74 total 100.00 100.00 ·program (1) The onion is diced in advance, the Sanxian gum and sugar are mixed and melted, and the spice and seasoning are mixed and melted. (2) Add olive oil to the pan, fry the onions, add the tomatoes and lightly overheat. (3) Add (1) and other raw materials to (2), and add spices and white wine. (4) (3) was put into a bag, and it heated at 90 degreeC for 40 minutes. (5) After cooling (4), it was heated gradually, and it was set as the sample for sensory evaluation. ・Evaluation method With regard to the samples obtained by the above procedure, two fully trained sensory inspectors performed sensory evaluations on a 5-level evaluation scale with regard to the fruitiness and acidity of the tomato sauce, with Blank set to 3 points, and obtained the results of each sensory evaluation. The average of the inspectors' evaluation points. ·Evaluation results The results are shown in the table. Inventive product 2 added strains were evaluated as follows: Compared with Blank, the fruitiness or acidity of tomato sauce was improved. [Table 34] sample name fruity sour Evaluation Blank 3 3 Sauce with a refreshing sour taste Test 1 5 4 Sauce with increased fruitiness of tomatoes [Example 24] Addition test to natto (inventive product 2) According to the following table and the following procedures, the effect of adding sterilized clarified salt-added lactic acid bacteria and soybean milk fermentation liquid (Invention 2) to natto was confirmed. [Table 35] Natto sauce [%] Raw material name Blank Test 1 light soy sauce 58.8 58.8 Light amino acid liquid 25.4 25.4 Glucose fructose liquid sugar 9.8 9.8 Sodium L-Glutamate 3.4 3.4 Dried Bonito Chip Extract Powder M-6* 0.5 0.5 IG-Na 2 0.1 0.1 Invention 2 - 0.2 salt 0.2 0.16 make water 1.8 1.64 total 100.00 100.00 *Made by Fuji Food Industry Co., Ltd. ·program (1) The raw materials described in the table were mixed to prepare a natto sauce. (2) To 50 g of natto (Okame Natto (manufactured by Takanofoods)), 3.7 g of the seasoning juice of (1) was added. It stirred 30 times with chopsticks to prepare a sample for sensory evaluation. ・Evaluation method With respect to the samples obtained by the above procedure, two fully trained sensory inspectors performed sensory evaluation on a 3-level evaluation with Blank set to +++ (3 points) for natto odor, and obtained each sensory inspection The average of the evaluation points of the members. ·Evaluation results The results are shown in the table. Inventive product 2 added strain was evaluated as follows: Compared with Blank, natto odor was suppressed. [Table 36] sample name Natto stinks Evaluation Blank +++ Has the unique smell of natto Test 1 + Difficult to feel that the smell of natto has been significantly reduced weak + <---> +++ strong

圖1係使用有乳清之乳酸菌/酵母醱酵物之春捲之評價結果(素材感) 圖2係使用有乳清之乳酸菌/酵母醱酵物之春捲之評價結果(風味之良好度) 圖3係使用有豆乳之乳酸菌/酵母醱酵物之春捲之評價結果(素材感) 圖4係使用有豆乳之乳酸菌/酵母醱酵物之春捲之評價結果(風味之良好度) 圖5係使用有乳清之乳酸菌/酵母醱酵物之中華蓋飯之配料之評價結果(素材感) 圖6係使用有乳清之乳酸菌/酵母醱酵物之中華蓋飯之配料之評價結果(風味之良好度) 圖7係使用有豆乳之乳酸菌/酵母醱酵物之中華蓋飯之配料之評價結果(素材感) 圖8係使用有豆乳之乳酸菌/酵母醱酵物之中華蓋飯之配料之評價結果(風味之良好度) 圖9係使用有豆乳之乳酸菌/酵母醱酵物之香烤雞塊之評價結果 圖10係使用有豆乳之乳酸菌/酵母醱酵物之香烤雞塊之破碎試驗之結果 圖11係使用有豆乳之乳酸菌/酵母醱酵物之豆乳之評價結果 圖12係應用使用有豆乳之乳酸菌/酵母醱酵物之植物蛋白質之漢堡肉餅的評價結果 圖13係使用有豆乳之乳酸菌/酵母醱酵物之日式煎餅醬汁之評價結果 圖14係使用有豆乳之乳酸菌/酵母醱酵物之熱巧克力之評價結果 圖15係使用有豆乳之乳酸菌/酵母醱酵物之奶油巧克力之評價結果 圖16係使用有豆乳之乳酸菌/酵母醱酵物之牛乳之光劣化臭抑制效果的評價結果 圖17係使用有豆乳之乳酸菌/酵母醱酵物之豆乳之光劣化臭抑制效果的評價結果(素材感) 圖18係使用有豆乳之乳酸菌/酵母醱酵物之味噌之評價結果Figure 1 shows the evaluation results of spring rolls using lactic acid bacteria/yeast starter with whey (sense of material) Figure 2 shows the evaluation results of spring rolls using lactic acid bacteria/yeast starter with whey (goodness of flavor) Figure 3 shows the evaluation results of spring rolls using lactic acid bacteria/yeast fermented with soymilk (sense of material) Figure 4 shows the evaluation results of spring rolls using lactic acid bacteria/yeast fermented product with soymilk (goodness of flavor) Figure 5 shows the evaluation results of the ingredients of the Chinese rice bowl using lactic acid bacteria/yeast fermented with whey (sense of material) Fig. 6 shows the evaluation results of ingredients of Chinese rice bowl using lactic acid bacteria/yeast fermented product with whey (goodness of flavor) Figure 7 shows the evaluation results of the ingredients of the Chinese rice bowl using lactic acid bacteria of soymilk/yeast fermented material (sense of material) Fig. 8 shows the evaluation results (goodness of flavor) of ingredients of Chinese rice bowl using lactic acid bacteria of soymilk/yeast fermented product Figure 9 shows the evaluation results of the fragrant roasted chicken nuggets using lactic acid bacteria/yeast fermented with soymilk Figure 10 shows the results of the crushing test of the fragrant roasted chicken nuggets using lactic acid bacteria/yeast fermented with soymilk Fig. 11 shows the evaluation results of soymilk using lactic acid bacteria of soymilk/yeast fermented product Fig. 12 shows the evaluation results of applying the hamburger patty using the lactic acid bacteria of soymilk/yeast fermented vegetable protein Figure 13 shows the evaluation results of Japanese pancake sauce using lactic acid bacteria/yeast fermented with soymilk Figure 14 shows the evaluation results of hot chocolate using lactic acid bacteria of soymilk/yeast leaven Figure 15 shows the evaluation results of cream chocolate using lactic acid bacteria of soymilk/yeast leaven Fig. 16 shows the evaluation results of the light deterioration odor suppression effect of milk using lactic acid bacteria of soymilk/yeast fermented product Fig. 17 shows the evaluation results of the light deterioration odor suppression effect of soymilk using lactic acid bacteria of soymilk/yeast fermented product (sense of material) Fig. 18 shows the evaluation results of miso using lactic acid bacteria of soymilk/yeast fermented product

[主張利用生物材料1]  [寄存國家] JP日本  [寄存機構]獨立行政法人製品評價技術基盤機構專利微生物寄存中心  [寄存日期] 2015/06/26  [寄存號碼] NITE BP-02072  [主張利用生物材料2]  [寄存國家] JP日本  [寄存機構]獨立行政法人製品評價技術基盤機構專利微生物寄存中心  [寄存日期] 2015/06/26  [寄存號碼] NITE BP-02073  [主張利用生物材料3]  [寄存國家] JP日本  [寄存機構]獨立行政法人製品評價技術基盤機構專利微生物寄存中心  [寄存日期] 2015/06/26  [寄存號碼] NITE BP-02074  [主張利用生物材料4]  [寄存國家] JP日本  [寄存機構]獨立行政法人製品評價技術基盤機構專利微生物寄存中心  [寄存日期] 2015/06/26  [寄存號碼] NITE BP-02075[Agreement to use biological materials 1] [Deposit country] JP Japan [Deposit institution] Patent Microorganism Deposit Center of Independent Administrative Corporation Product Evaluation Technology Base Agency [Deposit Date] 2015/06/26 [Deposit Number] NITE BP-02072 [Agreement to use biological materials Material 2] [Deposit country] JP Japan [Deposit agency] Patent Microorganism Deposit Center of Independent Administrative Incorporated Product Evaluation Technology Base Agency [Deposit Date] 2015/06/26 [Deposit Number] NITE BP-02073 [Proposition to Use Biological Materials 3] [ Deposit country] JP Japan [Deposit agency] Patent Microorganism Deposit Center, an independent administrative agency product evaluation technology base agency [Deposit date] 2015/06/26 [Deposit number] NITE BP-02074 [Proposition to use biological materials 4] [Deposit country] JP Japan [Deposit agency] Patent Microorganism Deposit Center of Independent Administrative Legal Person Product Evaluation Technology Base Agency [Deposit Date] 2015/06/26 [Deposit Number] NITE BP-02075

Claims (7)

一種醱酵調味料組合物,其係以豆乳之利用乳酸菌及酵母所產生之醱酵物(乳酸菌/酵母醱酵物)為有效成分之用於選自由畜肉或魚肉之軟化、豆乳之腥味遮蔽、植物蛋白質臭遮蔽、香辛料之刺激之增強、可可風味提昇、牛乳或豆乳之光劣化臭之抑制、乳感提昇、味噌之穀物風味之提昇、生魚之腥味及經加熱之魚之魚臭遮蔽、畜肉臭遮蔽、果實感提昇、及納豆臭遮蔽所組成之群中之任一者,乳酸菌/酵母醱酵物係藉由包含下列步驟之製造方法而獲得者:於將豆乳、葡萄糖及酵母萃取物混合、溶解之液中接種乳酸菌後,進行乳酸醱酵而獲得乳酸菌醱酵液;於所獲得之乳酸菌醱酵液中添加將葡萄糖、酵母萃取物及水混合、溶解之液,並接種酵母後,進行通氣攪拌醱酵而獲得酵母醱酵液;以及自所獲得之酵母醱酵液去除乳酸菌菌體及酵母菌體,而獲得乳酸菌/酵母醱酵物。 A fermented seasoning composition, which uses the fermented product (lactic acid bacteria/yeast fermented product) produced by using lactic acid bacteria and yeast of soybean milk as an active ingredient, and is used for softening of meat or fish, masking the fishy smell of soybean milk, and vegetable. Protein odor masking, spice stimulation enhancement, cocoa flavor enhancement, light deterioration odor suppression of milk or soy milk, milky texture enhancement, miso grain flavor enhancement, fish odor masking of raw fish and heated fish, livestock meat Any one of the group consisting of odor masking, fruitiness improvement, and natto odor masking, the lactic acid bacteria/yeast fermented product is obtained by a manufacturing method comprising the following steps: mixing soymilk, glucose and yeast extract, After inoculating the lactic acid bacteria in the dissolving liquid, carry out lactic acid fermentation to obtain a lactic acid bacteria fermentation liquid; add glucose, yeast extract and water to the obtained lactic acid bacteria fermentation liquid, mix and dissolve the liquid, and after inoculating the yeast, carry out aeration Stir and ferment to obtain yeast fermented liquid; and remove lactic acid bacteria cells and yeast cells from the obtained yeast fermented liquid to obtain lactic acid bacteria/yeast fermented product. 如請求項1之醱酵調味料組合物,其中乳酸菌為選自由鼠李糖乳酸桿菌(Lactobacillus rhamnosus)、乳酸小球菌(Pediococcus acidlactici)、融合乳酸桿菌(Lactobacillus confusus)、壞醱酵乳酸桿菌(Lactobacillus malefermentans)、副乾酪乳酸桿菌(Lactobacillus paracasei subsp.paracasei)、保加利亞乳酸桿菌(Lactobacillus delbrueckii subsp.bulgaricus)、乳酸乳球菌 (Lactococcus lactis)、嗜酸乳酸桿菌(Lactobacillus acidophilus)、及瑞士乳酸桿菌(Lactobacillus helveticus)所組成之群中之任一種。 The fermented seasoning composition of claim 1, wherein the lactic acid bacteria are selected from the group consisting of Lactobacillus rhamnosus , Pediococcus acidlactici , Lactobacillus confusus , Lactobacillus malefermentans ), Lactobacillus paracasei subsp.paracasei , Lactobacillus delbrueckii subsp.bulgaricus , Lactococcus lactis , Lactobacillus acidophilus , and Lactobacillus helveticus ) in any of the groups. 如請求項1之醱酵調味料組合物,其中酵母為選自由釀酒酵母菌(Saccharomyces cerevisiae)、清酒酵母菌(Saccharomyces sake)、白葡萄酒酵母菌(Saccharomyces beticus)、粟酒裂殖酵母菌(Schizosaccharomyces pombe)所組成之群中之任一種。 The fermented seasoning composition of claim 1, wherein the yeast is selected from Saccharomyces cerevisiae , Saccharomyces sake , Saccharomyces beticus , Schizosaccharomyces pombe ) any of the groups. 一種豆乳或以豆乳為原料之加工食品,其包含換算為固形物成分6.5%之乳酸菌/酵母醱酵物時而為0.4~1.0%之如請求項1至3中任一項中所定義之乳酸菌/酵母醱酵物。 A soymilk or a processed food using soymilk as a raw material, which contains 0.4 to 1.0% of lactic acid bacteria as defined in any one of Claims 1 to 3/ Yeast starter. 一種用於選自由畜肉或魚肉之軟化、豆乳之腥味遮蔽、植物蛋白質臭遮蔽、香辛料之刺激之增強、可可風味提昇、牛乳或豆乳之光劣化臭之抑制、乳感提昇、味噌之穀物風味之提昇、魚肉臭遮蔽、畜肉臭遮蔽、果實感提昇、及納豆臭遮蔽所組成之群中之任一者之方法,其包括如下步驟:添加如請求項1至3中任一項中所定義之乳酸菌/酵母醱酵物。 One for softening meat or fish meat, masking fishy odor of soybean milk, masking odor of vegetable protein, enhancing stimulation of spices, enhancing cocoa flavor, suppressing light-deteriorating odor of milk or soybean milk, enhancing milky feeling, and cereal flavor of miso A method of any one of the group consisting of enhancing, masking fish odor, masking animal odor, enhancing fruitiness, and masking natto odor, comprising the steps of: adding as defined in any one of claims 1 to 3 The lactic acid bacteria/yeast starter. 如請求項1至3中任一項之醱酵調味料組合物,其中有效成分包含基於乳酸菌/酵母醱酵物之分子量而區分之組分,且該組分之下限之分子量為75~1,800,上限之分子量為2,200~10,000。 The fermented seasoning composition according to any one of claims 1 to 3, wherein the active ingredient comprises a component differentiated based on the molecular weight of the lactic acid bacteria/yeast fermented product, and the molecular weight of the lower limit of the component is 75-1,800, and the upper limit of the component is 75-1,800. The molecular weight is 2,200~10,000. 一種用於選自由畜肉或魚肉之軟化、豆乳之腥味遮蔽、植物蛋白質臭遮蔽、香辛料之刺激之增強、可可風味提昇、牛乳或豆乳之光劣化臭之抑制、乳感提昇、味噌之穀物風味之提昇、生魚之腥味及經加熱之魚之魚臭遮蔽、畜肉臭遮蔽、果實感提昇、及納豆臭遮蔽所組成之群中之任一者之劑,其包含豆乳之利用乳酸菌及酵母所產生之醱酵物(乳酸菌/酵母醱酵物)之分子量500~3,500之組分,乳酸菌/酵母醱酵物係藉由包含下列步驟之製造方法而獲得者:將豆乳、葡萄糖及酵母萃取物混合、溶解後,接種乳酸菌並進行乳酸醱酵,而獲得乳酸菌醱酵液;於所獲得之乳酸菌醱酵液中添加將葡萄糖、酵母萃取物及水混合、溶解之液,並接種酵母後,進行通氣攪拌醱酵而獲得酵母醱酵液;以及自所獲得之酵母醱酵液去除乳酸菌菌體及酵母菌體,而獲得乳酸菌/酵母醱酵物。 One for softening meat or fish meat, masking fishy odor of soybean milk, masking odor of vegetable protein, enhancing stimulation of spices, enhancing cocoa flavor, suppressing light-deteriorating odor of milk or soybean milk, enhancing milky feeling, and cereal flavor of miso An agent of any one of the group consisting of enhancing the fishy odor of raw fish, masking the fish odor of heated fish, masking animal meat odor, enhancing fruit feeling, and masking the odor of natto, which includes the use of lactic acid bacteria and yeast in soymilk. The resulting fermented product (lactic acid bacteria/yeast fermented product) has a molecular weight of 500-3,500. The lactic acid bacteria/yeast fermented product is obtained by a manufacturing method comprising the following steps: mixing soymilk, glucose and yeast extract, and after dissolving , inoculate lactic acid bacteria and carry out lactic acid fermentation to obtain lactic acid bacteria fermentation liquid; add glucose, yeast extract and water mixing and dissolving liquid in the obtained lactic acid bacteria fermentation liquid, and after inoculating yeast, carry out aeration and stirring fermentation and obtaining a yeast fermented liquid; and removing the lactic acid bacteria and yeast cells from the obtained yeast fermented liquid to obtain a lactic acid bacteria/yeast fermented product.
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