TWI749777B - Ink-jet printing biological test sheet and method for making the same - Google Patents

Abstract

The disclosure provides a biological test sheet manufactured by an ink-jet printing process. The biological test sheet comprises a substrate and a supporting layer and a cover placed sequentially on the substrate. The substrate has a first electrode, a second electrode and a third electrode, which are formed on its surface and isolated from each other. The supporting layer has a first test chamber, a second test chamber and a channel inside, the first test chamber and the second test chamber are isolated from each other and communicate with outside through the channel. The first test chamber and the second test chamber separately have different biological agents inside. The biological test sheet of the disclosure has at least two test chambers, which separately have different biological agents, the test can be conducted and several biological characteristic data can be obtained at one time.

Description

Inkjet type biological test piece and preparation method thereof

The present invention relates to a biological test piece, in particular to a biological test piece with multiple detection areas prepared through an inkjet process.

As modern people’s diets become more abundant, chronic diseases caused by eating habits have become more common. This chronic disease has gradually replaced infectious diseases and has become one of the main causes of human death. Therefore, the demand for biological test strips to detect and monitor human biological characteristics, such as blood sugar, cholesterol, uric acid, etc., is becoming more and more urgent.

The traditional biological test strip using electrochemical principles is generally composed of a substrate with electrodes, an insulating layer with an isolation chamber, and an upper separator. In this traditional biological test strip, the insulating layer is generally an insulating material similar to the substrate, and there is only a single isolation chamber for blood testing. Since the insulating layer is attached to the substrate by bonding, it is impossible to miniaturize the biological test piece. Furthermore, the active enzyme and the insulating layer in the isolation chamber are also prepared separately, resulting in a complicated manufacturing process. The traditional biological test strip only has a single isolation room for testing. If it is necessary to detect the value of multiple biological characteristics, blood must be collected multiple times, and multiple biological test strips are used to test the blood in batches to obtain the biological characteristics value. As a result, it may be necessary to collect blood from the user multiple times, which is unfriendly to the user and uses a large amount of Biological test pieces, collecting biological characteristic values in batches, are also wasteful and not environmentally friendly. Driven by the current market demand, traditional biological test strips are increasingly unable to meet the needs of modern people.

Therefore, there is a need for a new and improved biological test piece to overcome the above-mentioned shortcomings of the prior art.

In view of this, the present invention provides an inkjet biological test strip. The biological test strip includes: a substrate with a first electrode, a second electrode, and a third electrode formed on the surface of the substrate, and the first electrode , The second electrode and the third electrode are isolated from each other; a support layer is arranged on the substrate, and has a first detection area, a second detection area and a channel therein, the first detection area and the second detection area The two detection areas are isolated from each other and communicate with the outside through the channel; and a cover plate is arranged on the support layer; wherein, the first detection area and the second detection area respectively contain different biological reagents. The inkjet biological test strip of the present invention has at least two detection areas, and the two detection areas contain different biological reagents, and multiple detections can be performed at one time to obtain multiple biological characteristic values. Therefore, in use, there is no need to collect multiple biological test samples from the patient, such as blood, as long as it is collected once, multiple biological characteristics such as blood sugar, blood lipids, cholesterol, uric acid, heme, etc. can be detected and obtained at the same time. The inkjet biological test strip with multiple detection areas of the present invention can be more convenient in use and more user-friendly.

Preferably, the first detection area and the second detection area of the inkjet biological test strip each include an opening penetrating the cover plate.

Preferably, part of the first electrode and part of the second electrode are exposed in the first detection area of the inkjet biological test strip, and a first biological body is covered on the exposed first electrode and the second electrode. Reagents.

Preferably, part of the first electrode and part of the third electrode are exposed in the second detection area of the inkjet biological test strip, and a second biological body is covered on the exposed first electrode and the third electrode. Reagents.

Preferably, the support layer of the inkjet biological test strip includes a stacked layer of UV ink, and has a surface tension in the range of 20-40 Dyne.

Preferably, the UV ink stack layer as the supporting layer of the inkjet biological test strip has a thickness ranging from 10 to 100 micrometers (μm).

Preferably, the biological reagent of the ink-jet biological test strip contains an enzyme, an antigen or an antibody.

Preferably, the biological reagent of the inkjet biological test strip has a surface tension in the range of 25-50 Dyne.

Preferably, the substrate surface of the inkjet biological test strip further includes a fourth electrode and a fifth electrode, which are separated from the first electrode, the second electrode, and the third electrode, respectively.

Preferably, the support layer of the inkjet biological test strip further includes a third detection zone and a fourth detection zone, which are separated from the first detection zone and the second detection zone respectively, and the third detection zone A portion of the first electrode and a portion of the fourth electrode are exposed inside, and a portion of the first electrode and a portion of the fifth electrode are exposed in the fourth detection area, and the first electrode and the fourth electrode are exposed in the third detection area. The electrode is covered with a third biological reagent, the first electrode and the fifth electrode exposed in the fourth detection area are covered with a fourth biological reagent, the first biological reagent, the second biological reagent, and the third biological reagent The reagent and the fourth biological reagent are different.

Another object of the present invention is to provide a method for preparing an inkjet biological test strip, including: providing a substrate; forming a first electrode, a second electrode, and a third electrode on the substrate, and the first electrode , The second electrode and the third electrode are isolated from each other; using an inkjet process, spray a support layer on the substrate, and expose part of the first electrode and part of the second electrode to form a first detection Area, exposing part of the first electrode and part of the third electrode to form a second detection area, and exposing part of the substrate surface to form a channel, wherein the first detection area and the second detection area are mutually Isolate and communicate with the outside through the channel; use the inkjet process to spray different biological reagents on the first detection zone and the second detection zone; and set a cover on the support layer.

Preferably, in the preparation method of the inkjet biological test strip, inkjetting the support layer includes a stacked layer completed by spraying multiple UV ink layers and curing by irradiating UV light by using the inkjet process.

Preferably, in the preparation method of the inkjet biological test strip, the UV ink layer sprayed by the inkjet process has a viscosity range of 2-30 centipoise (cP).

Preferably, in the preparation method of the inkjet biological test strip, the UV ink layer sprayed by the inkjet process has an ink volume between 3-100 ink droplets (pL).

Preferably, in the preparation method of the inkjet biological test strip, spraying different biological reagents on the first detection zone and the second detection zone includes using the inkjet process to spray separately in the first detection zone A first biological reagent, and a second biological reagent is sprayed in the second detection zone.

Preferably, the preparation method of the inkjet biological test strip further comprises using the spraying process to spray a hydrophilic material on the surface of the substrate in the channel.

Preferably, in the preparation method of the inkjet biological test strip, the biological reagent sprayed by the inkjet process has a viscosity in the range of 1-20 centipoise (cP).

Preferably, in the preparation method of the inkjet biological test strip, the biological reagent sprayed by the inkjet process has an ink volume between 1-100 ink droplets (pL).

Preferably, in the preparation method of the inkjet biological test strip, the inkjet process spraying different biological reagents includes spraying different biological reagents into the first detection area by means of a piezoelectric inkjet head. And the second detection zone.

In the preparation method of the inkjet biological reagent in an embodiment of the present invention, the support layer, the first detection area, the second detection area, the channel, the biological reagent, etc. can all be sprayed through the inkjet process, so it can be more precise In the area to be formed, the amount of material required for spraying can be simplified in a precise and precise manner, which is more convenient in the process and saves the use of materials, so it has the advantages of reducing production costs and so on. Furthermore, in the inkjet biological reagent preparation method of the embodiment of the present invention, the inkjet process used can be operated in a digital manner to control fine inkjet droplets, and it can also be obtained without using a semiconductor process. Biological test strips close to the microchip level.

2: Inkjet biological test strip

4: substrate

6: The first electrode

8: second electrode

10: Third electrode

12: Support layer

14: Channel

16: The first detection zone

18: The second detection zone

20: cover

22: opening

24: entrance

26: Biological reagents

28: Fourth electrode

30: Fifth electrode

32: The third detection zone

34: The fourth detection zone

Figure 1-2 is a schematic diagram of an inkjet biological test strip according to an embodiment of the present invention.

Fig. 3 is a schematic diagram of an inkjet biological test strip according to another embodiment of the present invention.

Fig. 4 is a cross-sectional view of the inkjet biological test strip of the embodiment in Fig. 3 taken along the line A-A'.

In order to understand the technical features and practical effects of the present invention in detail, and can be implemented in accordance with the content of the specification, the preferred embodiments shown in the drawings are further described in detail as follows.

Please cooperate with the preferred embodiments shown in FIGS. 1 to 4, the present invention provides an inkjet biological test strip. 1 and 2 are schematic diagrams of the inkjet biological test strip 2 according to an embodiment of the present invention. The inkjet biological test strip 2 in this embodiment refers to a biological test strip 2 prepared by an inkjet process. . In this embodiment, the inkjet biological test strip 2 includes a substrate 4, a supporting layer 12, and a cover plate 20, and can be composed of three layers stacked, but it is not limited to this. On the surface of the substrate 4, a first electrode 6, a second electrode 8 and a third electrode 10 are formed, and the first electrode 6, the second electrode 8 and the third electrode 10 may be separated from each other. In an embodiment, the above-mentioned substrate 4 may be an insulating material with a flat surface, such as polyethylene terephthalate (PET), polyvinyl chloride (PVC), glass fiber (FR-4), polyester, Polycarbonate (PC), polypropylene (PP), polyethylene (PE), ceramics, etc. or any combination of the above materials.

In the embodiment of FIG. 1, the first electrode 6 may be a T-shaped pattern, and the second electrode 8 and the third electrode 10 may be an I-shaped or straight pattern, and these electrodes may be formed by the substrate 4 One end extends to the other end, one end can be used to contact the test sample, and the other end can be used to connect to the test instrument. The electrode patterns in this embodiment are only used to illustrate the implementable aspects of the present invention. Those skilled in the art should change appropriate electrode patterns and configurations according to requirements. The electrode patterns shown in FIG. 1 are not intended to limit the present invention. In addition, the electrode in this embodiment can be any conductive material, such as cesium glue, platinum glue, gold glue, carbon glue, silver glue, copper glue, etc., or a combination thereof.

As shown in FIG. 1, the support layer 12 has a first detection area 16, a second detection area 18, and a channel 14. The first detection area 16 and the second detection area 18 may be detection areas isolated from each other, and pass through the channel 14 and the outside world. Connected, so that the test sample can be introduced into the test area. Next, a cover plate 20 with an opening 22 is provided on the support layer 12 to form an inkjet biological test strip according to the embodiment of the present invention. As shown in Figure 1, the opening 22 It can be respectively correspondingly arranged at the positions close to the bottom of the first detection zone 16 and the second detection zone 18, and can provide the detection zone to discharge gas. When the test sample is introduced from the outside, this opening can exhaust the gas in the first test 16 and the second test area 18, avoiding the gas in the test area from obstructing the introduction of the test sample, and helping to introduce the test sample to the test area Inside. In this embodiment, the opening 22 in FIG. 1 may be a square, which is respectively formed in the cover plate 20 and penetrates the cover plate 20, but in other embodiments, it may also be a circle, a rectangle or other suitable shapes. Alternatively, a slit may penetrate through the cover plate 20, so that it can be used to exhaust the gas in the detection area.

Fig. 2 is a partial schematic top view of the embodiment of Fig. 1. In FIG. 2 for convenience of description, some elements are omitted, for example, the cover plate 20 is omitted to show the detection area in the support layer 12 (as shown by the dashed line), and although in FIG. 2, the electrode is directly drawn, but this Just to show the electrode pattern of this embodiment, in fact, most of the first electrode 6, the second electrode 8 and the third electrode 10 can be covered by the support layer 12. As shown in Figure 2, the support layer 12 covers the surface of the substrate 4 and the first electrode 6, the second electrode 8, and the third electrode 10, and exposes part of the first electrode 6, the second electrode 8, the third electrode 10, and part of the The surface of the substrate 4 forms a first detection area 16, a second detection area 18, and a channel 14 respectively. Please refer to FIG. 2, the first detection area 16 and the second detection area 18 are isolated from each other to avoid mutual interference during detection, and communicate with the outside through the channel 14. That is to say, the first detection area 16 may be the exposed part of the first electrode 6 and the second electrode 8, and the exposed first electrode 6 and the second electrode 8 are covered with the biological reagent 26, and the second detection area 18 It may be that a part of the first electrode 6 and the third electrode 10 are exposed, and the exposed first electrode 6 and the third electrode 10 are covered with a biological reagent 26. In one embodiment, the above-mentioned biological reagent 26 may be an enzyme, and the biological reagent in the first detection area 16 and the biological reagent in the second detection area 18 may be different enzymes, so that after the detection sample is introduced, the biological reagent can be a one-time Carry out biological testing and obtain at least two biological characteristics at the same time.

As shown in FIG. 2, the inkjet biological test strip 2 according to the embodiment of the present invention further includes an inlet 24 for sucking the external test sample by capillary action, flowing through the channel 14, and respectively introducing the first test sample. In the area 16 and the second detection area 18. The opening 22 near the bottom of the first and second detection areas 16 and 18 will exhaust the gas in the detection area, which helps to introduce the test sample into the first and second detection areas 16 and 18. When the test sample, such as blood, is introduced into the first test area 16 and the second test area 18, the first electrode 6, the second electrode 8 and the third electrode 10 exposed at the other end can be connected to the test instrument (not (Shown), at this time, the first electrode 6 and the second electrode 8 and the detection instrument form an electrical circuit, and the first electrode 6 and the third electrode 10 may form an electrical circuit with the detection instrument. The biological reagent and the test sample in the first detection zone 16 generate electrical signals through a chemical reaction, and after the electrical signals are transmitted to the testing instrument through the electrodes, they are converted into biological characteristic values. Similarly, the test sample in the second test area 18 can also read the value of the biological feature through the test instrument. Since the biological reagents in the first detection area 16 and the second detection area 18 can be different, different biological characteristic values can be obtained at the same time, such as blood glucose, cholesterol, uric acid, lactic acid, hemoglobin, etc., but not This is limited.

In one embodiment, the enzyme of the biological reagent 26 may be an active substance, such as a glucose-like enzyme, an antigen, an antibody microbial cell, an animal and plant cell, and other components that have biorecognition capabilities. Moreover, in this embodiment, the biological reagent 26 may have a surface tension in the range of 20-50 dynes. In one embodiment, the biological reagent 26 may additionally include a conductive medium for receiving the electrons generated after the enzyme of the active material reacts with the test sample, and respectively conduct the electrons to the test instrument via the electrodes. In one embodiment, the above-mentioned conductive medium may be red blood salt, and the biological reagent may additionally include other materials, and is not limited thereto. It is worth mentioning that, in this embodiment, a hydrophilic material may also be formed on the surface of the substrate 2 in the channel 14, so as to facilitate the introduction of the test sample. While, in one In the embodiment, the supporting layer 12 can also be called a retaining wall to define and isolate the detection area, and the surface tension of the supporting layer 12 can preferably also be between 20-40 Dyne.

As shown in FIG. 2, the first detection area 16 and the second detection area 18 of this embodiment may be rectangular spaces separated by the support layer 12. In other embodiments, they may also be fan-shaped, circular, or elliptical. Or polygons, etc. Furthermore, the size of the electrode area exposed in the detection area only needs to be sufficient to conduct electrical signals, and it is not limited to that shown in FIG. 2. In addition, in one embodiment, the entrance 24 may also be trapezoidal in shape, which shows a tapering trend toward the channel 14 from the outside, so as to facilitate the introduction of the test sample into the test area.

FIG. 3 shows a top view of an inkjet biological test strip 2 according to another embodiment of the present invention. The main difference from the embodiment of FIGS. 1 and 2 is that the inkjet biological test strip 2 of FIG. 3 includes four detection areas (as shown by the dotted line). The materials and formation methods of the same component symbols can be referred to the above-mentioned embodiments. I won’t repeat it here. Similarly, FIG. 3 directly draws the electrodes in order to show the electrode patterns, but in fact, the supporting layer 12 may cover most of the electrodes. As shown in FIG. 3, there are a first electrode 6, a second electrode 8, a third electrode 10, a fourth electrode 28, and a fifth electrode 30 on the surface of the substrate 4. As in the above-mentioned embodiment, the first electrode 6 may be T-shaped The second, third, fourth, and fifth electrodes 8, 10, 28, 30 can be I-shaped or straight patterns, and the first electrode 6 can be used as a common electrode for each detection area. In other words, each detection area can share the first electrode 6 and be matched with other electrodes to perform biological sample detection. In this embodiment, the support layer 12 has a first detection area 16, a second detection area 18, a third detection area 32, and a fourth detection area 34, and the first detection area 16 may be the exposed part of the first electrode 6 and the second detection area. The second electrode 8, the second detection area 18 may be the exposed part of the first electrode 6 and the third electrode 10, the third detection area 32 may be the exposed part of the first electrode 6 and the fourth electrode 28, and the fourth detection area 34 may be A portion of the first electrode 6 and the fifth electrode 30 are exposed. Similarly, the first detection zone 16, the second detection zone 18, the third detection zone 32, and the fourth detection zone 34 may contain different biological reagents 26 for detecting and obtaining multiple biological reagents at one time. Characteristic value, for example Blood sugar, blood lipids, cholesterol, uric acid, lactic acid and heme, etc. As in the above embodiment, the first detection area 16, the second detection area 18, the third detection area 32, and the fourth detection area 34 may be isolated from each other, and communicate with the outside through the channel 14 to introduce the test sample. It can be understood that the number and shape of the electrodes, detection areas, channels, etc. in the present embodiment can be increased or decreased or adjusted and changed according to requirements, and the present invention is not limited to the aspect shown in the embodiment diagrams.

Fig. 4 is a cross-sectional view of the inkjet biological test strip 2 in the embodiment of Fig. 3 taken along the line AA'. In order to facilitate the display, only the first detection area 16 is shown as an example, and other detection areas may also have similar designs. As shown in FIG. 4, the method for preparing an inkjet biological test strip according to an embodiment of the present invention may include providing a substrate 4 and forming a first electrode 6 on the surface of the substrate 4. In this step, the second electrode 8, the third electrode 10, the fourth electrode 28 and the fifth electrode 30 may be formed simultaneously with the first electrode 6. Then, using an inkjet process, the support layer 12 is sprayed on the surface of the substrate 4 to cover part of the electrodes while exposing part of the electrodes to define the detection area. For example, an inkjet head (not shown) is used to spray a stack of multiple layers of UV ink materials on the substrate 4, respectively exposing parts of the first electrode 6, the second electrode 8, the third electrode 10, the fourth electrode 28, and the fifth electrode. The electrode 30 defines the first detection area 16, the second detection area 18, the third detection area 32 and the fourth detection area 34. In an embodiment, the support layer 12 may be a UV curable material, which is sprayed sequentially and irradiated with UV light to cure the stacked layers of UV ink layers. In a preferred embodiment, the supporting layer 12 may be, for example, a stacked layer of three layers of UV ink. The stacked layer may have a thickness ranging from 10 to 100 microns (μm), and the supporting layer 12 may have a surface tension. The range is between 20-40 dynes. In an embodiment, the UV ink material can also be a hydrophobic material, but it is not limited to this. In addition, in this embodiment, the UV ink layer sprayed by this inkjet process can be an ink with a viscosity range of 2-30 centipoise (cP), and the sprayed UV ink layer is ink The volume is between 3-100 ink droplets (pL).

As shown in Figure 4, next, using the same inkjet process, the first detection zone 16, the second detection zone 18, the third detection zone 32, and the fourth detection zone 34 are sprayed with a biological reagent 26 to cover the exposed first detection zone. An electrode 6, a second electrode 8, a third electrode 10, a fourth electrode 28, and a fifth electrode 30. In this embodiment, the inkjet process may use a piezoelectric inkjet head to spray the biological reagent 26 into the first detection area 16, the second detection area 18, the third detection area 32, and the fourth detection area, respectively. Inside the detection area 34. After that, the cover plate 20 is disposed on the supporting layer 12 to form an inkjet biological test strip 2. In one embodiment, during the inkjet process, hydrophilic materials can also be selectively injected into the channel 14. In an embodiment, the surface of the cover plate 20 facing the support layer 12 may also have an adhesive layer (not shown) to attach the cover plate 20 to the support layer 12. In one embodiment, the biological reagent sprayed by the inkjet process may be an ink with a viscosity range of 1-20 centipoise (cP), and the volume of the sprayed biological reagent ink is between 1 Between ~100 droplets (pL).

The inkjet biological test strip in the embodiment of the present invention refers to the preparation of a support layer, a plurality of isolated detection areas, biological reagents, etc. by an inkjet process. Since the required materials can be precisely controlled to prepare the support layer and biological reagents through the inkjet process, material waste can be avoided and production costs can be saved. In addition, the inkjet process can precisely control the size of the ink ejected. Therefore, it is possible to prepare biological test strips close to the microchip level without using a semiconductor process. Furthermore, the multiple detection areas of the embodiment of the present invention can share the first electrode or the main electrode, so the design is simpler and the manufacturing cost is more competitive.

The foregoing descriptions are only preferred embodiments of the present invention, and are not intended to limit the scope of rights claimed by the present invention. All other equivalent changes or modifications made without departing from the spirit disclosed by the present invention shall be included in the present invention. Within the scope of patent application.

2: Inkjet biological test strip

4: substrate

6: The first electrode

8: second electrode

10: Third electrode

12: Support layer

14: Channel

16: The first detection zone

18: The second detection zone

20: cover

22: opening

Claims (18)

An inkjet biological test strip, comprising: a substrate with a first electrode, a second electrode and a third electrode formed on the surface of the substrate, and the first electrode, the second electrode and the third electrode are mutually connected Isolation; a support layer is arranged on the substrate, and has a first detection area, a second detection area and a channel in which, the first detection area and the second detection area are isolated from each other, and through the channel and Communicating with the outside world; and a cover plate disposed on the support layer; wherein, the first detection area and the second detection area respectively contain different biological reagents; wherein, the support layer includes one of spraying using an inkjet process stacked layers composed of UV ink, UV ink, and the spray coating has a viscosity ranging between 2 ^to 30 centipoise (cP). The inkjet biological test strip according to claim 1, wherein the first detection area and the second detection area each include an opening penetrating the cover plate. The inkjet biological test strip according to claim 1, wherein part of the first electrode and part of the second electrode are exposed in the first detection area, and the first electrode and the second electrode are exposed A first biological reagent is covered on it. The inkjet biological test strip according to claim 1, wherein part of the first electrode and part of the third electrode are exposed in the second detection area, and the first electrode and the third electrode are exposed A second biological reagent is covered on it. The biological test strip ink jet request of the items 1, wherein the support layer comprises a stacked layer of the UV ink, and has between ²⁰ and 40 dynes (Dyne) a surface tension ranging. The requested item to item 5 of the ink jet biological specimen, wherein, the UV ink stacked layer having a thickness ranging from 10 ^to 100 micrometers ([mu] m) between. The inkjet biological test strip according to claim 1, wherein the biological reagent comprises an enzyme, an antigen or an antibody. The requested items 1 of the ink jet type of biological test strip, wherein the biological agent having a surface tension range between 25 ^to 50 dynes (Dyne). The inkjet biological test strip according to claim 1, wherein the substrate surface further includes a fourth electrode and a fifth electrode, which are respectively connected to the first electrode, the second electrode, and the third electrode. Electrode isolation. The inkjet biological test strip according to claim 7, wherein the support layer further includes a third detection area and a fourth detection area, and is separated from the first detection area and the second detection area, respectively , The third detection area exposes part of the first electrode and part of the fourth electrode, and the fourth detection area exposes part of the first electrode and part of the fifth electrode; wherein, the third detection area exposes the The first electrode and the fourth electrode are covered with a third biological reagent, the first electrode and the fifth electrode exposed in the fourth detection zone are covered with a fourth biological reagent, the first biological reagent, the second The biological reagent, the third biological reagent, and the fourth biological reagent are all different. An inkjet biological test strip preparation method, comprising: providing a substrate; forming a first electrode, a second electrode and a third electrode on the substrate, and the first electrode, the second electrode and the second electrode The three electrodes are isolated from each other; a support layer is sprayed on the substrate by an inkjet process, and a portion of the first electrode and a portion of the second electrode are exposed to form a first detection area, and a portion of the first electrode is exposed. The electrode and part of the third electrode form a second detection zone, and expose part of the substrate surface to form a channel, wherein the first detection zone and the second detection zone are isolated from each other and pass through the channel. External communication; using the inkjet process to spray different biological reagents on the first detection zone and the second detection zone; and set a cover on the support layer; wherein the support layer includes UV ink layer of a multilayer coating formed, the UV ink and the sprayed layer having a viscosity ranging from 2 ^to 30 centipoise (cP) between. The method for preparing an inkjet biological test strip according to claim 11, wherein spraying the support layer includes spraying the multi-layer UV ink layer by using the inkjet process and curing by irradiating UV light A stacked layer. The method as in items 12 of the ink jet biological specimen request, wherein the inkjet process is the sprayed layer of the UV ink droplet having between 3 ^{and 100} (pL) between a volume of ink. The method for preparing an ink-jet biological test strip according to claim 11, wherein spraying different biological reagents on the first detection zone and the second detection zone includes using the inkjet process to respectively apply different biological reagents to the first detection zone A first biological reagent is sprayed in a detection area, and a second biological reagent is sprayed in the second detection area. The method for preparing an inkjet biological test strip as described in claim 11 further comprises using the spraying process to spray a hydrophilic material on the surface of the substrate in the channel. The method of preparing an ink jet biological test strip of the requested item, such as item 11, wherein the ink jet process using the spray coating a biological agent having a viscosity ranging between ¹ and 20 centipoise (cP). The method of preparing an ink jet biological test strip of the requested item, such as item 11, wherein the ink jet process using the spray coating the biological agent having a volume of ink droplet is between 1 ^{and 100} (pL). The method for preparing an inkjet biological test strip according to claim 11, wherein the inkjet process spraying different biological reagents includes spraying different biological reagents by means of a piezoelectric inkjet head. Into the first detection zone and the second detection zone.

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