CN1296180A - Biochemical sensor with multifunction sampling mode - Google Patents

Abstract

The biochemical detector for detecting biochemical component content consists of electrically-insulating substrate, on the substrate there are anode portion and cathode portion, and a working electrode and anode connector and a reference electrode and cathode connector are respectively formed on its two ends: a reaction layer on working electrode and reference electrode, said reaction layer can be contacted with the detected body to produce chemical reaction; and electrically-insulating layer on the substrate, it has a notch for receiving detected body to reaction layer and its opened end; and a reticular covering layer on the reaction layer for at least covering partial notch. The detected body utilizes the capillary attraction force between reticular wvering layer and reaction layer to add sample from side notch or if the reticular covering layer is hydrophilic layer, the sample can be added from upper surface of test block.

Description

The biochemical sensor of tool multifunction sampling mode

The present invention relates to a kind of biochemical sensor, more specifically, relate to a kind of in order to detect the current type sheet biochemical sensor of corpse or other object for laboratory examination and chemical testing mesophytization component content or concentration.

Biochemical Analyzer generally can be divided into three kinds of wet type, dry type and biochemical sensors; The wet type Biochemical Analyzer is that the inspection product are mixed with reagent (containing developer usually), carries out chemical reaction, again with optics interpretation device tintmeter for example, and the change color before and after surveys such as spectrophotometer are read.Ce Shi inspection product can not use whole blood in this way, therefore must be through pre-treatment, and instrument is expensive, and the layman can't operate and wait restriction, therefore, be commonly used in hospital and check use.The dry type Biochemical Analyzer then is at test piece surface coated chemical reagent, and enzyme or antibody etc. directly contact with the inspection product, analyze, and have saved reagent preparation and method of operating and have simplified, but still measured with the principle that is color method; The easy oxidation stain of the test piece of this analyser is subject to inspection product color interference, therefore also can't use whole blood.

Biochemical sensor is made up of biological elements, thin-film component and sensor, and biological elements wherein is that a kind of microorganism, cell, tissue, enzyme, antigen or antibody etc. of having comprised have the biomaterial of specificity identification capability; Thin-film component is generally macromolecular material, in order to fix aforesaid biological elements and to screen out interfering material; Sensor then comprises electrode, and crystal, thermal resistor, piezo-electric device, optical fiber, photoelectric tube and sound wave counter etc. are imitated in the ion selectivity field, and wherein the hydrogen peroxide electrode is one of at present most widely used biochemical sensing transducer.

Biochemical sensor with analysis blood sugar is an example, its principle is the hydrogen peroxide electrode surface that is fixed in glucose oxidase on the film and this film tightly is placed in column, bestow polarized potential in platinum anode and silver/silver chloride negative electrode again, make the generation hydrogen peroxide via the reaction of glucose oxidase enzymatic glucose oxidase, hydrogen peroxide can continue to be oxidized to water near anode surface, and disengage electronics simultaneously, can extrapolate the concentration of glucose of inspection in the product according to the emission of electronics.

Aforesaid columnar electrode is polishing often, and mantle is difficult for, cleans difficulty, therefore easily cross pollution, be difficult for to proofread and correct, be difficult for making disposable type, and shortcoming such as cost of manufacture is higher, actual use inconvenience, therefore the exploitation of test piece shape electrode is just arranged,, be beneficial to industrialization and make to overcome the shortcoming of columnar electrode.

United States Patent (USP) 5,120,420 disclose a kind of sheet biochemistry detection electrode, comprise electrode part, insulation course, active layer, and be positioned at this active layer top cause corpse or other object for laboratory examination and chemical testing bearing space with resin plate and hydrophilic nmature upper cover plate, wherein this corpse or other object for laboratory examination and chemical testing bearing space must have a corpse or other object for laboratory examination and chemical testing suction inlet and a gas discharge outlet; The wherein formation of this active layer, be partly to be covered with carboxy methyl cellulose (CMC) aqueous solution in this electrode base board in regular turn, dry back forms one deck CMC layer, continue to spray glucose oxidase solution (GOD) thereon, dry then, spray the organic phase suspending liquid that contains conducting medium more thereon, give drying and just finish biochemical reaction zone, at last in being covered with on the resin plate that causes corpse or other object for laboratory examination and chemical testing bearing space and the hydrophilic material cover plate on the substrate to finish this tabular biochemistry detection electrode.

The shortcoming of this biochemistry detection electrode is: first, finish the action-reaction district and need three steps: form hydrophobicity, formation GOD floor and the formation conducting medium of CMC floor in order to improve the carbonaceous electrodes surface, and the first drying of palpus before each step is finished, step is numerous and diverse: second, after sampling mode is the tip of contact electrode test piece, the product of will examining import reaction zone, and sample tap has only a position.

United States Patent (USP) 5,628,890 disclose another kind of sheet biochemistry detection electrode, comprise an electrode part, an insulation course, an active layer, are positioned at the water wettability Double-level Reticulated of this active layer top, and the last cap rock that covers Double-level Reticulated.Its electrode is a three-pole, prints carbon-coating earlier and prints silver layer again.The work utmost point in the reaction zone, with reference to the utmost point with the utmost point is covered with bioactivator, silver/silver chloride and conducting medium respectively again.The two-layer screen cloth of coating more subsequently, this bilayer screen cloth is necessary for hydrophilic treatment, with the importing of convenient sample, add hydrophilic Double-level Reticulated and work as blood sample, easily be limited on the active layer by the affinity effect of hydrophilic net, one deck loam cake of fitting again on screen cloth afterwards stays a sample tap in last cap rock.Above-mentioned detecting electrode is the production process complexity not only, screen cloth multilayer and need hydrophilic treatment, and sample tap has only one, when adding test piece, blood sample can't differentiate to import reaction zone whether really, and blood sample demand height, more than 10 microlitres (μ l).

The present invention is promptly at the shortcoming of aforementioned prior art, reacted constituent is mixed, once add responding layer, and above the test piece sample sucks breach, add the netted overlayer of last layer, can between netted overlayer and responding layer, produce capillary force, breach by the test piece next door adds sample, makes sampling easily, or if the netted hydrophilic layer that is covered as, sample can be added by the test piece top by netted covering mat water wettability, therefore provides a kind of making simple, easy to detect and effective biochemical sensor.

One object of the present invention is to provide a kind of biochemical sensor, and it has polynary sample position, can a corpse or other object for laboratory examination and chemical testing drips to put or make test piece carry out the sampling of a corpse or other object for laboratory examination and chemical testing near a corpse or other object for laboratory examination and chemical testing as the mode near the sagging place of human body ear therefore very convenient sampling.

The present invention's another purpose is to provide a kind of biochemical sensor, and it can import responding layer with a corpse or other object for laboratory examination and chemical testing easily and promptly, carries out detection reaction effectively to obtain a corpse or other object for laboratory examination and chemical testing.

Another object of the present invention is to provide a kind of biochemical sensor, and it can reduce the sampling amount of a corpse or other object for laboratory examination and chemical testing, and blood sampling volume further reduces patient's pain below 5 microlitres.

The present invention's a more purpose is to provide a kind of easy biochemical sensor of making, and it must the layering drying, can form an even smooth biological responding layer on the surface, and still can reach the requirement of accuracy.

For achieving the above object and avoiding the shortcoming of prior art, the invention provides a kind of biochemical sensor, in order to detect the content that corpse or other object for laboratory examination and chemical testing mesophytization becomes branch, comprise: an electrically insulating base; An anode part that is positioned on this substrate, its two ends form a working electrode and a positive contact respectively; A cathode portion that is positioned on this substrate, its two ends form a reference electrode and a negative contact respectively; One deck is positioned at the responding layer on this working electrode and this reference electrode, in order to contact with a corpse or other object for laboratory examination and chemical testing to produce chemical reaction; One deck is positioned at the electric insulation layer on this substrate, and it has one in order to hold this corpse or other object for laboratory examination and chemical testing to the breach of this responding layer and the openend of a breach; And one deck is positioned at the netted overlayer on this reaction synusia, and it is this breach of cover part at least; By this, this corpse or other object for laboratory examination and chemical testing can be by the water wettability of this netted overlayer by adding sample above the netted overlayer or by the openend arrival responding layer of the capillary force that is produced between netted overlayer and responding layer by this breach.Wherein should the reaction series of strata be formed by the prescription of enzyme, carrier and electron mediator (mediator) and surfactant.

The netted overlayer of biochemical sensor of the present invention is water wettability Web materials or hydrophobic reticulated material or wire netting, and it provides capillary force easily and quickly and effectively the openend importing responding layer of a corpse or other object for laboratory examination and chemical testing by biochemical sensor next door breach detected.

The openend of this breach is as the contact point of a corpse or other object for laboratory examination and chemical testing, can in order near and contact patient's corpse or other object for laboratory examination and chemical testing sampling place on one's body, make a corpse or other object for laboratory examination and chemical testing enter responding layer and detect by the openend of this breach; As long as biochemical sensor near the patient, just can effectively be obtained a corpse or other object for laboratory examination and chemical testing, sampling is convenient.In a preferred embodiment, this substrate also has the teat of a semicircle in the openend lower edge of this breach, with contact point as a corpse or other object for laboratory examination and chemical testing, this semicircular teat not only be easy to debate know and can be more near sampling spot with convenient sampling, and the sensation when releiving patient's touch sensor.

By announcement of the present invention, manufacturer is produced biochemical sensor easily, the reviewer is able to easy and obtain effectively that a corpse or other object for laboratory examination and chemical testing detects, patient's inconvenience is reduced to minimum and can access testing result accurately, is to serve multiple.

State before the present invention and other purposes, advantage and the technology contents how to reach, will be described further according to the following detailed description conjunction with figs., in the accompanying drawing:

Fig. 1 is the STRUCTURE DECOMPOSITION figure according to the biochemical sensor of one embodiment of the invention;

Fig. 2 is the structure outside drawing of this embodiment;

Fig. 3 is a view on this embodiment;

Fig. 4 is the sectional view of this embodiment at the A of Fig. 3 section; And

Fig. 5 is the comparison diagram as a result according to blood sugar concentration in biochemical sensor of one embodiment of the invention and the YSI blood sugar analyzer detection blood.

Please refer to Fig. 1 to 4, according to the biochemical sensor 1 of one embodiment of the invention, in order to detect the content that corpse or other object for laboratory examination and chemical testing mesophytization becomes branch, this biochemical sensor comprises: an electrically insulating base 2; An anode part 3 that is positioned on this substrate 2, its two ends form a working electrode 4 (as shown in Figure 3) and a positive contact 5 respectively; A cathode portion 6 that is positioned on this substrate 2, its two ends form a reference electrode 7 (as shown in Figure 3) and a negative contact 8 respectively.

This base material 2 have straight surface, electrical isolation characteristic and can be anti-40 ℃～200 ℃ temperature capacities of heating and handling, handle so that heat and increase this anode part 3 and cathode portion 6 electrical conductivities and tack, the material of the base material 2 that is suitable for includes but not limited to PVC plate, polyester, bakelite plate, glass mat (FR-4) plate, PET plate, PC, PP, PE, PA, PS plate, glass plate or pottery (CEM-1).

This anode part 3 and cathode portion 6 are two separates and mutual discontiguous conducting film, in order to be connected in the sensing apparatus (not shown).

Anode part 3 covers one deck electric insulation layer 10 and 10 in part, afterwards, exposed two ends be respectively in order to the positive contact 5 that connects sensing apparatus with and on be coated with the working electrode 4 (please refer to Fig. 3) of one deck as the bioactive layer of responding layer 9, the electrical effect that is brought out when chemistry or the biochemical reaction in order to a conduction corpse or other object for laboratory examination and chemical testing is to this sensing apparatus.Cathode portion 6 covers one deck electric insulation layer 10 and 10 in part, afterwards, exposed two ends then be respectively this in order to the negative contact 8 that connects sensing apparatus with and on be coated with a reference electrode 7 (please refer to Fig. 3) as the biochemical activity layer of responding layer 9, detect in order to the electrical effect of 4 pairs of corpse or other object for laboratory examination and chemical testing of cooperating electrode.

The present invention's biochemical sensor 1 comprises a responding layer 9 (please refer to Fig. 4) that is positioned on this working electrode 4 and this reference electrode 7 in addition, and it is one deck biochemical activity layer, in order to contact with a corpse or other object for laboratory examination and chemical testing to produce chemical reaction.Wherein this responding layer is not overlapping with aforementioned electric insulation layer.

This responding layer 9 is placed by the compositing formula of a biochemical active substance that the back is formed on the cathode electrode of substrate 2, and this responding layer is made up of the prescription of enzyme, carrier, electron mediator and surfactant.In specific embodiment, this carrier use amount is the weight 0.05% to 1.5% of prescription, and the carrier that can use has ultra micron cellulose, methylcellulose (MC), carboxy methyl cellulose (CMC), starch, vinyl alcohol, vinyl pyrrolidone, polyvinyl alcohol (PVA) (PVA), polyvinyl pyrrolidone (PVP), polyglycol (PEG) or gelatin (gelatin) etc.

Available compositing formula is as follows:

Enzyme, as glucose oxidase, use amount 200～1200U/ml

Enzymatic protective reagent uses 0.1% to 1.0% of formulation weight the most, and available protective agent has: albumin, dextrin, dextran, amino acid etc., above-mentioned protective agent can use one or multinomial mixing to use separately;

Electron mediator, use amount are 2.0% to 10.0% of formulation weight, and available conducting medium has potassium ferricyanide (potassium ferricyanide);

Surfactant, account for below 0.1% of formulation weight, the surfactant that can use has alkyl benzene polyglycol ether (Triton X-100), Triton X-405, Triton X-114, sodium dodecyl sulfate solution (sodium lauryl sulfate), polyoxyethylene sorbitan monolaurate (polysorbas20), polysorbate40, polysorbate60, Tween 80 or other water soluble surfactant active or detersive.

The present invention's biochemical sensor 1 comprises an electric insulation layer 10 and 10 that is positioned on this substrate 2 in addition, and it has one in order to hold this corpse or other object for laboratory examination and chemical testing to the breach 11 (0.25～0.3 millimeter of height) of this responding layer 9 and the openend 12 of a breach.This electric insulation layer can be selected from PP, PVC, PET, PC or PE.

This electric insulation layer 10 and 10 ' after covering upper substrate 2, except make exposed one the Dragon Boat Festival utmost point part 3 and cathode portion 6 form positive contact 5 and negative contact 8 respectively, more in the other end by this breach in 11 zones of being limited to form working electrode 4 and reference electrode 7 respectively.In preferred embodiments, the area of this working electrode 4 is 2 to 3 times of area of reference electrode 7.

The zone and the responding layer 9 of this breach below 11 of being limited to by this breach 11 form so-called conversion zone, to hold a corpse or other object for laboratory examination and chemical testing and to react.The thickness of the electric insulation layer 10 of conversion zone top is generally 0.25 to 0.30 millimeter.

The present invention's biochemical sensor 1 also comprises one deck and is positioned at netted overlayer 13 on this electrical insulation sheet 10, and in order to the capillary action of protective reaction layer and a promotion and a corpse or other object for laboratory examination and chemical testing, it is this breach 11 of cover part at least; By this, this corpse or other object for laboratory examination and chemical testing can be by the openend 12 arrival reaction retes 9 of the capillary force that is produced between this netted overlayer 13 and the responding layer 9 by this breach.

This netted overlayer 13 can be selected from water wettability Web materials or hydrophobic reticulated material or wire netting, and its mesh number includes but not limited to 60 to 300 orders.When netted overlayer 13 was made up of the water wettability Web materials, for a hydrophilic corpse or other object for laboratory examination and chemical testing, a corpse or other object for laboratory examination and chemical testing can be by 12 importings of gap opened end or by netted overlayer opening top, as a direction importing of Fig. 4.When netted overlayer 13 is made up of the hydrophobic reticulated material, for a hydrophilic blood corpse or other object for laboratory examination and chemical testing, its compatibility a little less than, so a corpse or other object for laboratory examination and chemical testing mainly is to be imported by the openend 12 mat capillarity of breach.This netted overlayer also can be the hydrophobic reticulated material through the surfactant processing, and a corpse or other object for laboratory examination and chemical testing can be imported via a or the b direction of Fig. 4; Wherein this surfactant is selected from Triton X-100, Triton X-405, Triton X-114, sodium dodecyl sulfate solution, polyoxyethylene sorbitan monolaurate (polysorbas20), polysorbate40, polysorbate60, Tween 80 or other water soluble surfactant active or detersive.

Biochemical sensor 1 of the present invention has two with the mode that a corpse or other object for laboratory examination and chemical testing imports responding layer 9, when netted overlayer is water wettability, because the water wettability effect that the netted overlayer 13 of water wettability is provided, a corpse or other object for laboratory examination and chemical testing can be dripped by first corpse or other object for laboratory examination and chemical testing importing direction a as shown in Figure 4 to be put, or import by second corpse or other object for laboratory examination and chemical testing introducing port direction b mat capillary action, direction imports thus, biochemical sensor can be directly adjacent to a corpse or other object for laboratory examination and chemical testing, a corpse or other object for laboratory examination and chemical testing is able to be inhaled toward responding layer 9 rapidly and all sidedly, not only guaranteed effective testing result, and can be reduced dripping high-volume of a corpse or other object for laboratory examination and chemical testing; Moreover, when if netted overlayer is hydrophobicity, a corpse or other object for laboratory examination and chemical testing also can import direction b by second corpse or other object for laboratory examination and chemical testing and enter responding layer 9, this mode is that biochemical sensor is directly adjacent to patient body, for example under patient's ear, make and ooze little drop of blood with fine needle aspiration, and with close this drop of blood of biochemical sensor, make drop of blood import direction b by second corpse or other object for laboratory examination and chemical testing and enter responding layer 9, so the sampling quantity of a corpse or other object for laboratory examination and chemical testing can drop to minimum.

Diabetic's blood sampling volume when using blood glucose test is many, especially more serious patient often will carry out repeatedly sampling monitoring, if can reduce on the blood demand in one day, just can use more very thin blood taking needle, make patient's wound and pain reduce to minimum.Moreover, smear test piece when blood sample is added on the electrode test piece easily because blood volume is few, and then cause the error in the measurement.But the blood demand of the present invention's blood sugar lowering electrode test piece when measuring, and the mode of improvement blood-sample withdrawal.

In a preferred embodiment, the teat 14 that the substrate 2 of this biochemical sensor 1 also has a semicircle in openend 12 lower edges of this breach is with the contact point as a corpse or other object for laboratory examination and chemical testing.This semicircular teat can not only make it more near sampling spot with convenient sampling, and the sensation when releiving patient's touch sensor.

Be different from prior art, as United States Patent (USP) 5,120,420 and 5,628,890, the present invention adopts individual layer screen cloth structured material to be attached to the reaction zone top, owing to have a lot of holes to be easy to generate capillarity on the screen cloth material, therefore cause blood sampling easily, no matter be that hydrophobicity or water wettability screen cloth structured material all can be used in above the electrode test piece reaction zone as biochemical sensor at gap opened end 12 places of reaction zone.If netted when being covered as hydrophobicity, sample can be sucked by the b direction, if water wettability, sample can be sucked by a direction or b direction.

The manufacture process of the pellet electrode of biochemical sensor 1 of the present invention is simple, more can improve quality; Its step of making flow process is as follows in regular turn:

Step 1, at first on arbitrary flat surface of 1 a slice flat-shaped substrate 2, print off the conducting film that one deck includes an anode part 3 and a cathode portion 6 with screen printing technology, the material of conducting film can be the conductive slurry-like material that is fit to screen painting, as carbon paste, elargol, gold size or silver-colored carbon epoxy glue or its combination (as printing carbon paste again behind the seal elargol), oven dry under 40～150 ℃ then;

Step 2, with film adhered technology, on the aforementioned same surface that is printed with conducting film, the layer thickness that is covered be 0.1 to 1.0 millimeter electric insulation layer 10 ', and lining one layer thickness is 0.25～0.30 millimeter on the reaction zone position, and electric insulation layer 10 with openend 12 of a breach 11 and a breach, and reserve part is exposed and the conducting film in the zone of being limited for this breach 11 in order to forming working electrode 4 and reference electrode 7, and the zone of this working electrode 4 and reference electrode 7 part formation promptly is called reaction zone;

Step 3, dry down in 40～60 ℃ environment after the zone of aforementioned reaction zone drips bioactivator, to form a responding layer 9; And

Step 4 is coated on netted overlayer 13 on this responding layer 9 again.

Below, describe the present invention in detail with specific embodiment.

Embodiment 1

On a flat surface of a PC plate substrate, print off the carbon paste conducting film of an independent unconnected anode and one deck negative electrode, oven dry under 130 ℃ then with half tone.The conducting film that the PET electric insulation layer that surface-coated last layer thickness is 0.27mm in the same side that is printed on conducting film, and reserve part immediately exposes is to form positive contact, negative contact, working electrode and reference electrode.This working electrode and the zone that reference electrode formed are conversion zone.

The droplets of material that will contain following prescription composition and ratio afterwards is added on this conversion zone surface to form a responding layer:

Glucose oxidase 0.63%

Albumin 0.5%

Potassium ferricyanide 6%

Methylcellulose 0.5%

Triton X-100 (uncle's Octylphenoxy gathers ethanol) 0.07%

Phosphate buffer (pH5.0) 92.30%

After the reaction zone mid point adds above-mentioned bioactivator, place 50 ℃ to dry 15 minutes down test piece, being covered on responding layer immediately, (Te Duolong T120-54), makes the biochemical sensor of a current type electrode test piece to one deck Web materials.

Obtained jettisonable current type electrode test piece, b direction by Fig. 4 sucks sample, carry out the content measuring of blood sugar in the blood with whole blood, result such as Fig. 5, test piece and YSI blood sugar analyzer with the present invention detect comparison of blood sugar concentration in the blood, the present invention's test piece CV value detects required blood volume below 4 microlitres (μ 1) below 5%.Hence one can see that, and the measurement of biochemical sensor of the present invention is accurate.

Embodiment 2

It is as follows to change prescription as embodiment 1:

Glucose oxidase 0.63%

Albumin 0.5%

Potassium ferricyanide 6%

Carboxy methyl cellulose 0.5%

Triton X-100 (uncle's Octylphenoxy gathers ethanol) 0.07%

Phosphate buffer (pH5.0) 92.30%

Embodiment 3

It is as follows to change prescription as embodiment 1:

Glucose oxidase 0.63%

Albumin 0.5%

Potassium ferricyanide 6%

Glucosan 0.5%

Triton X-100 (uncle's Octylphenoxy gathers ethanol) 0.07%

Phosphate buffer (pH7.0) 92.30%

Embodiment 4

It is as follows to change prescription as embodiment 1:

Glucose oxidase 0.63%

Albumin 0.5%

Potassium ferricyanide 6%

Glutamic acid 0.1%

Polyglycol (PEG) 0.3%

Polysorbas20 0.1%

Phosphate buffer (pH6.0) 92.37%

Embodiment 5

Replace with hydrophobic reticulated material dacron PES-37T as embodiment 1.Its production process such as embodiment 1 after responding layer is fixedly finished, adhere to Web materials PES-37T on the electric insulation layer, finish an electrode test piece.

Embodiment 6

Replace with Web materials stainless steel cloth (100 order to 300 order) as embodiment 1.Specimen preparation program such as embodiment 1 after the reaction rete is fixedly finished, adhere to the Web materials stainless steel cloth on the electric insulation layer, finish an electrode test piece.

Embodiment 7

Replace the PC plate as embodiment 1 with the PVC plate, and replace with many Web materials of hydrophobicity dacron PES-42T.Specimen preparation program such as embodiment 1 after the reaction rete is fixedly finished, adhere to Web materials PES-42T on the electric insulation layer, finish an electrode test piece.

Embodiment 8

Handle via soaking 1%TritonX-100 with the Web materials PET-43T of hydrophobicity as embodiment 1, make it to become water wettability.After the reaction rete is fixedly finished, will adhere on the electric insulation layer, finish an electrode test piece, by a or the b direction suction sample of Fig. 4 through the Web materials PET-43T of 1%TritonX-100 hydrophilic treatment.

That disclosed biochemical sensor is not only made is easy, testing result is accurate, and has polynary sample position, can a corpse or other object for laboratory examination and chemical testing drips to put or carry out the sampling of a corpse or other object for laboratory examination and chemical testing near the mode of a corpse or other object for laboratory examination and chemical testing, convenient sampling; A corpse or other object for laboratory examination and chemical testing can be easily and is promptly imported responding layer, carries out detection reaction effectively to obtain a corpse or other object for laboratory examination and chemical testing; And can reduce the sampling amount of a corpse or other object for laboratory examination and chemical testing, with further minimizing patient's pain.For manufacturer, reviewer and patient, can both provide most convenient and effective solution.

Method of the present invention and feature fully are disclosed in the above-mentioned example explanation; Will be appreciated that any following of spirit of the present invention that do not break away from is modified or change for it, all should be covered by in the present invention's the protection domain.

Claims (15)

1, a kind of biochemical sensor in order to detect the content that corpse or other object for laboratory examination and chemical testing mesophytization becomes branch, comprises:

An electrically insulating base;

An anode part that is positioned on this substrate, its two ends form a working electrode and a positive contact respectively;

A cathode portion that is positioned on this substrate, its two ends form a reference electrode and a negative contact respectively;

One deck is positioned at the responding layer on this working electrode and this reference electrode, in order to contact with a corpse or other object for laboratory examination and chemical testing to produce chemical reaction;

One deck is positioned at the electric insulation layer on this substrate, and it has one in order to hold this corpse or other object for laboratory examination and chemical testing to the breach of this responding layer and the openend of a breach; And

A netted overlayer that is positioned on this responding layer, it is this breach of cover part at least.

2, biochemical sensor as claimed in claim 1, wherein this netted overlayer is water wettability Web materials or hydrophobic reticulated material or wire netting.

3, biochemical sensor as claimed in claim 2, wherein this netted overlayer has 60 to 300 orders.

4, biochemical sensor as claimed in claim 1, wherein this netted overlayer is the hydrophobic reticulated material through the surfactant processing.

5, biochemical sensor as claimed in claim 4, wherein this surfactant is selected from TritonX-100, Triton X-405, Triton X-114, sodium dodecyl sulfate solution, polysorbas20, polysorbate40, polysorbate60, Tween 80 or other water soluble surfactant actives or detersive.

6, biochemical sensor as claimed in claim 1, wherein this substrate also has a semicircular teat in the openend lower edge of this breach, with the contact point as a corpse or other object for laboratory examination and chemical testing.

7, biochemical sensor as claimed in claim 1, wherein this responding layer is made up of the prescription of enzyme, carrier, electron mediator and surfactant.

8, biochemical sensor as claimed in claim 7, wherein this carrier is ultra micron cellulose, methylcellulose, carboxy methyl cellulose, starch, vinyl alcohol, vinyl pyrrolidone, polyvinyl alcohol (PVA), polyvinyl pyrrolidone, polyglycol or gelatin.

9, biochemical sensor as claimed in claim 8, wherein this carrier composition accounts for 0.05% to 1.5% of formulation weight.

10, biochemical sensor as claimed in claim 7, wherein this electron mediator is a potassium ferricyanide.

11, biochemical sensor as claimed in claim 7, wherein this surfactant is Triton X-100, Triton X-405, Triton X-114, sodium dodecyl sulfate solution, polysorbas20, polysorbate40, polysorbate60, Tween 80 or other water soluble surfactant active or detersive.

12, biochemical sensor as claimed in claim 7, wherein this surfactant comprise formulation weight below 0.1%.

13, biochemical sensor as claimed in claim 1, wherein the area of this working electrode is 2 to 3 times of area of reference electrode.

14, biochemical sensor as claimed in claim 1, wherein this electric insulation layer is PP, PVC, PET, PC, PE or other insulation plastic material.

15, biochemical sensor as claimed in claim 1, wherein the thickness of this electric insulation layer is between 0.25 to 0.35mm.

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