TWI738209B - Strip detecting device - Google Patents

Abstract

A strip detecting device includes a platform, a test strip, a colorimetric board, and a mobile communication apparatus. The platform has a containing slot. The test strip is contained in the containing slot and reacts with a sample to generate a color block. The colorimetric board has a window, a plurality of calibration blocks formed at two sides of the window, and three positioning patterns formed around the calibrations blocks. When the colorimetric board covers the platform, the window exposes the color block. The mobile communication apparatus captures an original image corresponding to the color block, performs coordinate calibration on the original image to generate a calibration image according to the three positioning patterns, and compares the calibration blocks with the color block in the calibration image to generate a test result.

Description

Test piece detection device

The invention relates to a test strip detection device, in particular to a test strip detection device that uses mobile communication equipment to perform image positioning correction and test strip colorimetric calibration.

With the development of medical testing technology, many applications of fast screening test strips have emerged, such as urine test strips, fecal occult blood and gastric Helicobacter pylori test strips, etc., so as to help users quickly check their own health conditions. initial understanding. The common detection method is to contact the corresponding test strip (such as urine ten-item test strip, lateral flow chromatography immunoreaction test strip, etc.) with the specimen to be tested, and then compare the color plate and the test strip. The color to determine the test result.

However, the prior art usually judges the test result by visually comparing the color displayed by the contrast plate and the test piece. This method will not only cause a time-consuming and labor-intensive manual comparison process, but also easily cause visual colorimetric interpretation. The problem of error, although the use of image recognition methods has been developed to replace the above-mentioned visual colorimetric method for more accurate and objective interpretation of the test results, but because this method usually requires the user to take the colorimetric plate and the test piece by himself Therefore, problems such as image position deviation and skew, angle tilt, shaking, etc. often occur, which causes image interpretation errors and greatly affects the accuracy of the detection results.

Therefore, one of the objectives of the present invention is to provide a test piece detection device that uses mobile communication equipment to perform image positioning correction and test piece colorimetric correction to solve the above-mentioned problems.

According to one embodiment, the test strip detection device of the present invention includes a carrier, a test strip, a colorimetric plate, and a mobile communication device. The carrier has a containing groove structure. The test piece containing In the accommodating groove structure and reacting with a sample, at least one color detection block is generated. The colorimetric plate has a colorimetric window, a plurality of correction color blocks, and at least three first positioning patterns. The plurality of correction color blocks are formed on both sides of the colorimetric window. The at least three first positioning patterns are formed on the Around multiple correction color blocks. When the colorimetric plate covers the carrier, the colorimetric window is superimposed on the accommodating groove structure to expose the color detection block. The mobile communication device has an image capture unit and a processing unit. The image capture unit is used to capture an original image corresponding to the colorimetric plate that exposes the detected color block, and the processing unit is based on the original image Perform image coordinate correction on the images corresponding to at least three first positioning patterns to generate a positioning correction image, and perform colorimetric correction on the images corresponding to a plurality of correction color blocks in the positioning correction image and the detected color blocks to generate One test result.

In summary, compared with the prior art, the present invention uses the image capture function of the mobile communication device to capture images of the colorimetric plate and the test strip placed on the stage in real time, and uses the positioning of the colorimetric plate The pattern is corrected for the image coordinates. In this way, the test piece detection device provided by the present invention can not only obtain the detection result immediately without going through tedious detection procedures and lengthy waiting, but also can solve the problems mentioned in the prior art. The image position offset and skew, angle tilt, shaking and other problems caused by the user's own shooting greatly reduce the possibility of image interpretation errors, thereby effectively improving the detection accuracy of the test piece detection device.

The advantages and spirit of the present invention can be further understood from the following detailed description of the invention and the accompanying drawings.

10,100: Test piece detection device

12: Stage

14,102: Test strip

16,104: colorimetric plate

18: Mobile communication equipment

20: accommodating tank structure

22, 105: Detect color blocks

24,106: colorimetric window

26, 108: Correction of color blocks

28,110: the first positioning pattern

30: Image capture unit

32: processing unit

34: quick response code pattern

112: The second positioning pattern

114: Positioning color block

Figure 1 is a top view of a test strip detection device according to an embodiment of the present invention.

Figure 2 is a schematic diagram of the unfolding of the stage shown in Figure 1.

FIG. 3 is a top view of a test strip detection device according to another embodiment of the present invention.

Please refer to Figures 1 and 2. Figure 1 is a top view of a test strip detection device 10 according to an embodiment of the present invention, and Figure 2 is an expanded schematic view of a carrier 12 in Figure 1, as As shown in Figures 1 and 2, the test strip testing device 10 includes a carrier 12 (simply shown by a dashed line in Figure 1), a test strip 14, a colorimetric plate 16, and a mobile communication device 18 (It is briefly shown as a functional block diagram in Figure 1, and its related design is common in the prior art, so it will not be repeated here). The carrier 12 can preferably use model paper with a thickness of about 1 mm as the material (but not limited to this) and can have a accommodating groove structure 20 after assembly, because of its certain strength, low price, and design at the same time The upper part is integrally formed for the user to assemble.

The detection test strip 14 is housed in the accommodating groove structure 20 and generates at least one detection color block 22 after reacting with the sample (two are shown in the first figure, but not limited by this, the number of detection color blocks is The end depends on the actual detection application of the test strip detection device 10). In more detail, in this embodiment, the detection test strip 14 can preferably be a lateral flow chromatography immunoreaction test paper ( However, it is not limited to this). During the test, if the test result is positive, the test strip 14 will show the test consisting of two upper and lower C lines (control line) and T line (result lne) as shown in Figure 1. On the contrary, if the test result is negative, the test strip 14 will show a color block 22 consisting of a single C line, where the appearance of the C line indicates that the reagents and operations are normal and effective, and the T line is It will show different shades of color according to the concentration of fecal occult blood.

The colorimetric plate 16 may have a colorimetric window 24, a plurality of correction color blocks 26, and at least three first positioning patterns 28 (three are shown in the first figure and can preferably form three sides of the colorimetric plate 16 respectively In the corner position, but not limited by this), a plurality of correction color patches 26 (eight shown in the first figure, but not limited by this) are formed on both sides of the colorimetric window 24, and the first positioning pattern 28 is It can be formed around a plurality of correction color blocks 26, whereby, as shown in Figure 1, when the colorimetric plate 16 covers the carrier 12, the colorimetric window 24 is superimposed on the accommodating groove structure 20 to expose Detected color blocks 22; more specifically, in this embodiment, the colorimetric plate 16 can be drawn by drawing software (such as PhotoShop, etc.) and made by printing, and the first positioning pattern 28 can be used as a coordinate correction circle In order to locate and correct the coordinates, a plurality of correction color blocks 26 can be symmetrical in two rows as shown in the first figure. The arrangement is set on both sides of the colorimetric window 24, and each row of correction color blocks 26 can contain four different grayscale values for correcting the difference between the detection color block 22 and its background value, so as to obtain the detection color block 22 The actual grayscale value is used for the subsequent interpretation of the fecal occult blood concentration.

The mobile communication device 18 may preferably be a smart phone and has an image capturing unit 30 (such as a mobile phone front or rear lens) and a processing unit 32 (such as a mobile phone microprocessor), and the image capturing unit 30 It can be used to capture the original image corresponding to the colorimetric plate 16 that exposes the detected color block 22, and the processing unit 30 can perform image coordinate correction based on the image corresponding to the first positioning pattern 28 in the original image to generate a positioning correction image , And can perform colorimetric calibration based on the images corresponding to the plurality of correction color patches 26 and the detected color patches 22 in the corrected positioning correction image to generate the corresponding detection results.

Through the above design, when the user wants to perform the detection operation of the test strip detection device 10, the user only needs to first load the detection test strip 14 that has touched or dropped into the sample to produce the detection color block 22 into the carrier 12 In the accommodating groove structure 20, the colorimetric plate 16 is covered on the stage 12 to expose the color detection block 22 (as shown in Figure 1).

Next, the user can use the image capture unit 30 of the mobile communication device 18 to capture the original image of the corresponding colorimetric plate 16 exposing the detected color block 22. At the same time, the processing unit 32 can be based on the original image The image coordinate correction corresponding to the image of the first positioning pattern 28 is performed to generate a corresponding positioning correction image, so as to avoid problems such as image position shift, skew, angle tilt, and shaking when the user uses the mobile communication device 18 to shoot. In short, the processing unit 32 can find the center position and radius of the coordinate correction circle of the first positioning pattern 28 in the original image (such as using the Hough circle conversion detection method, but not limited by this), and then determine three Whether the relative positions of the two coordinate correction circles are correct (if there is a situation where the radius difference of the three coordinate correction circles is too strong, the user is required to take another shot), and the coordinate correction is performed accordingly, so as to accurately locate a plurality of The relative position of the correction color block 26 and the detection color block 22 is used for subsequent image identification and comparison.

Finally, the processing unit 32 can target a plurality of correction color patches 26 and detect color patches 22 Perform colorimetric calibration to generate corresponding detection results. As long as it is an image recognition method that can be used to perform colorimetric calibration on a plurality of calibration color patches 26 and detection color patches 22 to obtain the detection density of detection color patches 22, All of them can be used in the present invention. For example, the processing unit 32 can convert the RGB value of the positioning correction image to the HSV color space, and use the regression line and the Otsu algorithm to obtain the detection result from the HSV data. The position of the color patch 22, and the four different grayscale values represented by each row of correction color patches 26 are used to correct the difference between the detected color patch 22 and its background value to obtain the actual grayscale value of the detected color patch 22, so In other words, the concentration of the sample can be calculated based on the linear relationship between the gray scale value and the concentration to smoothly determine the accurate fecal occult blood concentration, thereby completing the detection operation of the test strip detection device 10.

It should be noted that since the processing unit 32 can accurately compare the actual grayscale value of the detected color block 22, even if the sample concentration is zero, the processing unit 32 can still detect the actual grayscale value of the color block 22 Value judgement reads the test result of zero-concentration fecal occult blood. In addition, in order to increase the computing speed and facilitate storage of records, the processing unit 32 can upload the image to the server for cloud computing to perform colorimetric calibration to generate detection results, and receive the computing results returned by the server for storage. Enter the database in the relevant application to record the detection date and concentration for users to browse and use in the future. In addition, in practical applications, in order to further avoid detection errors and increase the operation speed, as shown in Figure 1, the colorimetric plate 16 may additionally have a Quick Response Code pattern 34, whereby the processing unit 32 Image decoding can be performed based on the image of the quick response code pattern 34 in the original image to quickly obtain the detection-related information (such as detection type and detection result data) of the detection test strip 14.

In summary, compared with the prior art, the present invention uses the image capture function of the mobile communication device to capture images of the colorimetric plate and the test strip placed on the stage in real time, and uses the positioning of the colorimetric plate The pattern is corrected for the image coordinates. In this way, the test piece detection device provided by the present invention can not only obtain the detection result immediately without going through tedious detection procedures and lengthy waiting, but also can solve the problems mentioned in the prior art. The image position offset and skew, angle tilt, shaking and other problems caused by the user’s own shooting greatly reduce the possibility of image interpretation errors, thereby effectively improving the inspection of the test piece. The detection accuracy of the measuring device.

It is worth mentioning that the design of the colorimetric plate may not be limited to the above-mentioned embodiment. For example, please refer to Fig. 3, which is a top view of a test strip inspection device 100 according to another embodiment of the present invention. The elements mentioned in this embodiment and the above-mentioned embodiments have the same number, which represents the same or similar structure and function, and the related description is not repeated here. As shown in FIG. 3, the test strip detection device 100 includes a carrier 12 (indicated by a dashed line in FIG. 3 ), a test strip 102, a colorimetric plate 104, and a mobile communication device 18. The detection test strip 102 is placed on the stage 12 and produces corresponding detection color blocks 105 after reacting with the specimen (ten are shown in Figure 3, but it is not limited by this. The number of detection color blocks is the end It depends on the actual detection application of the test strip detection device 100). In more detail, in this embodiment, the detection test strip 102 can be ten urine test strips (such as leukocyte esterase, nitrite, and urobilin). , Protein, pH, occult blood reaction, specific gravity, ketones, bilirubin, and glucose testing), the urine test strip is a simple and convenient test method, it can assist doctors in diagnosis and disease tracking, the urine test strip is quick and easy to use. The price is cheap, and the test can be done with only a few urine samples, and different shades of color can be presented through different detection color blocks 105 according to the concentration content of different items in the urine sample.

The colorimetric plate 104 may have a colorimetric window 106, a plurality of correction color patches 108, and at least three first positioning patterns 110 (three are shown in Figure 3, but not limited to this), and a plurality of correction color patches 108 (The fifty-five calibration color patches 108 located in the dotted area are shown in Figure 3, but are not limited to this) are formed on both sides of the colorimetric window 106, and the first positioning pattern 110 can be formed on a plurality of calibration Around the color block 108, as shown in Figure 3, the color comparison plate 104 can be superimposed on the carrier 12 to expose the color detection color block 105 through the color comparison window 106; more specifically, here In an embodiment, the colorimetric plate 104 can be drawn by drawing software (such as PhotoShop, etc.) and made by printing. The first positioning pattern 110 can be a coordinate correction circle for positioning and correcting coordinates, and a plurality of correction color blocks 108 can be As shown in Figure 3, the ten rows are arranged on both sides of the colorimetric window 104. Each row of calibration color patches 108 may contain different grayscale values corresponding to each urine test to correct and detect the color patches 105. The difference between the background value and the background value, so as to obtain the actual detection color block 105 The international grayscale value is used for the interpretation of the subsequent urine test results.

It should be noted that, in this embodiment, in order to further improve the accuracy of positioning correction, as shown in Figure 3, the colorimetric plate 104 may additionally have at least one second positioning pattern 112 (shown in Figure 3) And the three first positioning patterns 110 are respectively formed on the four corners of the colorimetric plate 104, but not limited to this), the shape of the first positioning pattern 110 (such as a circle) is different from the second positioning pattern 112 The shape (such as a square). In addition, the colorimetric plate 104 may additionally have a plurality of positioning color blocks 114 (the thirty-three positioning color blocks 114 located in the dotted area are shown in Figure 3, but are not limited to this), and a plurality of positioning colors The block 114 is formed around the plurality of calibration color blocks 108, whereby the processing unit 32 can more accurately locate the positions of the plurality of calibration color blocks 108 according to the images corresponding to the plurality of positioning color blocks 114 in the original image.

Through the above-mentioned design, when the user wants to perform the detection operation of the test strip detection device 100, the user only needs to first load the test strip 102 that has touched or dropped into the sample to produce the detection color block 105 into the carrier 12 The detection color block 105 is exposed through the colorimetric window 106 (as shown in FIG. 3).

Next, the user can use the image capture unit 30 of the mobile communication device 18 to capture the original image of the corresponding colorimetric plate 104 that exposes the detected color patch 105. At the same time, the processing unit 32 can be based on the original image Perform image coordinate correction corresponding to the images of the first positioning pattern 110 and the second positioning pattern 112 to generate corresponding positioning correction images, so as to avoid image position shift, skew, angle tilt, etc. when the user uses the mobile communication device 18 to shoot. Problems such as shaking. In short, the processing unit 32 can find the center position and radius of the coordinate correction circle of the first positioning pattern 110 in the original image (if the Hough circle conversion detection method is used, but not limited to this) and the second positioning pattern 112 The center of the outline of the coordinate calibration square (if the Canny edge detection method is used, but it is not limited), and then determine whether the relative position of the coordinate calibration circle and the coordinate calibration square is correct (if there is a positioning pattern difference, the detection is too strong In the case of modification, the user is required to re-shoot), and the coordinate correction is performed accordingly, and the position of the boundary of the color block 114 is captured and positioned according to the corrected new coordinates, so as to accurately locate a plurality of correction color blocks 108 and detect The relative position of the color block 105 is used for subsequent image identification and comparison.

Finally, the processing unit 32 can perform colorimetric calibration for the plurality of correction color blocks 108 and the detection color block 105 to generate corresponding detection results, and as long as it can be used for the plurality of correction color blocks 108 and the detection color block 105 The method of performing colorimetric calibration to obtain the detection density of the detection color patch 105 can be adopted in the present invention. For example, the processing unit 32 can convert the RGB value of the positioning correction image to the HSV color space, and From the HSV data, the position of the detected color block 105 is obtained by regression line and Otsu's algorithm, etc., and the different grayscale values represented by each row of correction color blocks 108 are used to correct each detected color block 105 and its background value Obtain the actual gray-scale value of the color block 105 detected by the difference between, so that the concentration of the sample can be calculated based on the linear relationship between the gray-scale value and the concentration, and the concentration of each urine test can be judged smoothly, thereby completing the test piece test The detection operation of the device 100. As for the related description of other designs of the test strip detection device 100 (such as quick response code design, cloud computing design, etc.), it can be deduced by analogy with the above-mentioned embodiment, and will not be repeated here.

The foregoing descriptions are only preferred embodiments of the present invention, and all equivalent changes and modifications made in accordance with the scope of the patent application of the present invention shall fall within the scope of the present invention.

10: Test piece detection device

12: Stage

14: Test strip

16: Colorimetric plate

18: Mobile communication equipment

22: Detect color blocks

24: colorimetric window

26: Correction of color blocks

28: The first positioning pattern

30: Image capture unit

32: processing unit

34: quick response code pattern

Claims (6)

A test strip detection device, comprising: a carrier with a containing groove structure; a detection test strip, which is accommodated in the containing groove structure and reacts with a sample to produce at least one detection color block ; A colorimetric plate, which has a colorimetric window, a plurality of correction color blocks, and at least three first positioning patterns, the plurality of correction color blocks are formed on both sides of the colorimetric window, the at least three first positioning patterns Formed around the plurality of calibration color blocks, when the colorimetric plate covers the carrier, the colorimetric window is superimposed on the accommodating groove structure to expose the color detection color block; and a mobile communication device , It has an image capture unit and a processing unit. The image capture unit is used to capture one of the original images corresponding to the colorimetric plate exposing the detected color block, and the processing unit searches for the at least three of the original images. The center position and radius of the coordinate correction circle of the first positioning pattern, and then the image coordinate correction is performed according to the result of judging whether the relative position of each coordinate correction circle to each other is correct to generate a positioning correction image, and the pair in the correction image is corrected according to the positioning A plurality of calibrated color patches and the image of the detected color patch should be colorimetrically calibrated to generate a detection result. The test strip detection device according to claim 1, wherein the processing unit performs colorimetric calibration in a cloud computing manner to generate the detection result. The test piece detection device according to claim 1, wherein the colorimetric plate further has a plurality of positioning color blocks, the plurality of positioning color blocks are formed around the plurality of correction color blocks, and the processing unit is based on the original image The positioning of the plurality of calibration color blocks is performed corresponding to the images of the plurality of color positioning blocks. The test strip detection device according to claim 1, wherein the at least three first positioning patterns are respectively formed on three corner positions of the colorimetric plate. The test strip detection device according to claim 1, wherein the colorimetric plate further has at least one second positioning pattern, the at least one second positioning pattern is formed around the plurality of calibration color blocks, and the at least three first positioning patterns The shape of the pattern is different from the shape of the at least one second positioning pattern. The test piece detection device according to claim 1, wherein the colorimetric plate further has a quick response code pattern, and the processing unit performs image decoding according to the image corresponding to the quick response code pattern in the original image to obtain the detection test Information about the detection of the film.

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