CN113791065A - Test paper based on two-dimensional coding positioning and interpretation method - Google Patents
Test paper based on two-dimensional coding positioning and interpretation method Download PDFInfo
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- 238000012360 testing method Methods 0.000 title claims abstract description 107
- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 48
- 239000000758 substrate Substances 0.000 claims abstract description 16
- 238000012937 correction Methods 0.000 claims description 6
- 239000011159 matrix material Substances 0.000 claims description 6
- 230000001360 synchronised effect Effects 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 8
- 230000008569 process Effects 0.000 abstract description 8
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 210000002700 urine Anatomy 0.000 description 4
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000009535 clinical urine test Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 2
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- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
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- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06K—GRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
- G06K7/00—Methods or arrangements for sensing record carriers, e.g. for reading patterns
- G06K7/10—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation
- G06K7/10544—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation by scanning of the records by radiation in the optical part of the electromagnetic spectrum
- G06K7/10821—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation by scanning of the records by radiation in the optical part of the electromagnetic spectrum further details of bar or optical code scanning devices
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Abstract
The invention discloses a test paper based on two-dimensional coding positioning and an interpretation method, wherein the test paper comprises the following components: a test paper substrate; a positioning structure functional pattern based on two-dimensional codes is arranged on the test paper substrate, and at least one chemical reagent block for detecting a sample is arranged. In the using process, a coordinate system can be determined by utilizing the positioning structure functional pattern of the two-dimensional code, and the position of the chemical reagent block in the standard coordinate system is further identified; the interpretation result of the detection sample can be obtained by reading the RGB values of the corresponding positions. The test paper color block has a simple structure and is convenient to read.
Description
Technical Field
The invention relates to the field of two-dimensional code application and medical inspection analysis, in particular to test paper based on two-dimensional code positioning and an interpretation method.
Background
Currently, dry chemical test paper is a common method for medical clinical examination. The test results were obtained by manual colorimetry. The automatic analyzer and the semi-automatic analyzer use a color sensor to identify the color of the test paper after reaction and judge the inspection result, and strict precision requirements are provided for the size and the position of a color block of the test paper and the relative position relationship between the test paper block and the sensor in the identification process. The test patches are generally arranged in sequence by using a queue.
Patent document CN109900688A discloses a test paper arranged in a matrix, each test paper block is surrounded by a black frame of 1mm, after the image of the test paper block is read by a CCD camera sensor, 16 black frames are first found from the image file, and then the colors in the black frames are read, and if 16 black frames are not found, the identification fails.
For another example, when a certain household urine test APP is used for judging a test paper result, the test paper and a lens are required to be straight and parallel when photographing. If the position of the test paper is not correct, the accuracy of the interpretation result is affected.
However, how to provide a more convenient test strip and accurately and conveniently read the test result is a problem that practitioners of the art need to solve.
Disclosure of Invention
In view of the above problems, the present invention provides a test strip based on two-dimensional coding positioning and an interpretation method, which at least solve some of the above technical problems, the test strip has a simple structure, can be read accurately and conveniently, and solves the problems of complicated test strip color block structure and complicated reading process in the prior art.
In a first aspect, an embodiment of the present invention provides a test paper based on two-dimensional coding positioning, including:
a test paper substrate;
the test paper substrate is provided with a positioning structure functional pattern based on two-dimensional codes, and at least one chemical reagent block for detecting a sample is arranged.
In one embodiment, when the two-dimensional code is a matrix two-dimensional code, the positioning structure function pattern includes: a registration pattern, a synchronization pattern, an alignment pattern, and a white balance weight.
In one embodiment, the test paper substrate is further provided with a format data pattern; the format data pattern is used for storing at least one of the following information:
test paper model, test paper batch number or error correction code.
In one embodiment, when the two-dimensional code is a linearly stacked two-dimensional code, the positioning structure function pattern includes: a positioning pattern and a synchronization pattern.
In one embodiment, the chemical reagent block is located in the two-dimensional encoded internal data field; or the chemical reagent blocks are arranged in the region outside the two-dimensional code according to a preset rule.
In a second aspect, an embodiment of the present invention further provides an interpretation method for test paper based on two-dimensional coding positioning, which is used for interpreting the test paper according to any one of the embodiments; the interpretation method comprises the following steps:
obtaining a whole image based on two-dimensional coding positioning test paper;
identifying two-dimension code information of the image through a two-dimension code identification terminal;
reading RGB values of the chemical reagent blocks according to the two-dimension code information;
and comparing the RGB numerical values with a corresponding judgment result threshold value table, and outputting an interpretation result of the test paper.
In one embodiment, identifying two-dimensional code information of the scanned image comprises:
when the two-dimensional code is a matrix two-dimensional code, identifying a positioning pattern, a synchronous pattern, an alignment pattern, a white balance block, a format data pattern and a chemical reagent block;
when the two-dimensional code is a linear stacked two-dimensional code, a positioning pattern, a synchronization pattern, and a chemical reagent patch are identified.
In one embodiment, further comprising:
after the test paper model or the test paper batch number corresponding to the format data pattern is identified, searching a corresponding judgment result threshold value table; and when the two-dimensional code scanning terminal does not have the judgment result threshold value table, downloading the corresponding judgment result threshold value table through an OTA.
The technical scheme provided by the embodiment of the invention has the beneficial effects that at least:
the embodiment of the invention provides test paper based on two-dimensional coding positioning and an interpretation method, wherein the test paper comprises the following components: a test paper substrate; a positioning structure functional pattern based on two-dimensional codes is arranged on the test paper substrate, and at least one chemical reagent block for detecting a sample is arranged. In the using process, a coordinate system can be determined by utilizing the positioning structure functional pattern of the two-dimensional code, and the position of the chemical reagent block in the standard coordinate system is further identified; the interpretation result of the detection sample can be obtained by reading the RGB values of the corresponding positions. The test paper color block has a simple structure and is convenient to read.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.
The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
fig. 1 is a schematic structural diagram of test paper based on two-dimensional coding positioning according to embodiment 1 of the present invention;
fig. 2 is a schematic structural diagram of test paper based on two-dimensional coding positioning according to embodiment 2 of the present invention;
fig. 3 is a schematic view of a positioning test paper structure of a linear stacked two-dimensional code according to embodiment 3 of the present invention;
fig. 4 is a method for interpreting test paper based on two-dimensional code positioning according to embodiment 4 of the present invention.
Fig. 5 is a schematic diagram of an RGB three-dimensional rectangular coordinate system according to an embodiment of the invention.
Detailed Description
Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
Example 1:
referring to fig. 1, the test paper based on two-dimensional coding positioning according to the embodiment of the present invention includes a test paper substrate 1, a positioning structure function pattern 2 based on two-dimensional coding is disposed on the test paper substrate 1, and at least one chemical reagent block 3 for detecting a sample is disposed. The positioning structure functional pattern 2 may be disposed on the test paper substrate 1 by printing, inkjet, or the like, and the chemical reagent block 3 may be disposed on the test paper substrate 1 by, for example, pasting.
The test paper is of a two-dimensional code structure, the two-dimensional code is a matrix type two-dimensional code, and the test paper is formed by arranging and combining a positioning structure functional pattern 2 and a chemical reagent block 3 according to a specific format. Wherein the position of the chemical reagent block 3 determines the recognition rule for reading the scanned two-dimensional code.
The positioning structure function pattern 2 element comprises a positioning structure and format data pattern 21;
the positioning structure includes: the positioning pattern 22, the synchronization pattern 23, the alignment pattern 24, and the white balance weight 25, the pattern shape and the arrangement method thereof directly transfer to the design of the two-dimensional code.
As shown in fig. 1, the synchronization pattern: the angle of the two-dimensional code can be conveniently determined and the distortion can be corrected by the aid of the two black and white lines. The alignment pattern is used for correcting the graph, only exists above the QR Code 2 version, consists of three small square nests with black and white alternated, and is convenient for determining the center and correcting the distortion.
Positioning the pattern: the two-dimensional code is composed of three black-white large square nests which are respectively positioned at the upper left corner, the upper right corner and the lower left corner of the two-dimensional code, and the purpose is to determine the size and the position of the two-dimensional code.
The format data pattern 21, as shown in fig. 1, includes two sets of the same, which can record test strip ID information such as: test paper model, batch number and/or error correction code, etc. Each test paper model or test paper batch number corresponds to a group of chemical reagent blocks, and each group of chemical reagent blocks has different quantity or different arrangement sequence. According to the information of the format data pattern 21, the terminal scanning device can realize the scanning and identification of various two-dimensional codes. The error correction code may help the QR code reader detect and correct errors in the QR two-dimensional code.
The white balance block 25 contains information of a white balance correction block (position coordinates and side length information of a block center point) in the test paper, and is mainly used for eliminating the influence of color temperature of a light source on color identification.
As shown in fig. 1, the chemical reagent block 3 is located in the data field between the positioning pattern 22 and the alignment pattern 24, is used for detecting different components of the sample, can be composed of a corresponding number of reagent blocks according to different numbers of components of the detection object, and can be randomly arranged on the test paper substrate. The number and the size of the color blocks of the reagent are related to the detection principle of a detected sample; for example, the pregnancy test paper only needs two reagent color blocks; for another example, the urine test paper has 14 detection indexes, and then 14 reagent color blocks are needed. The size of the reagent color block is determined by the reaction principle of the components in the sample and the reagent color block, for example, the reagent color block which is easy to react and easy to change color can be adopted. The reagent patch size can be increased when it is desired to increase the reaction contact area.
The position and function of the reagent color block on the test paper are described by the following two data tables:
TABLE 1 color lump position table of certain type of test paper
| Number of color block | Center point coordinate X | Center of a shipPoint coordinate Y | Length of side in X direction | Length of side in Y direction |
| 1 | X1 | Y1 | lX1 | lY1 |
| 2 | X2 | Y2 | lX2 | lY2 |
| … | ||||
| N | XN | YN | 1XN | 1YN |
TABLE 2 result threshold judging table for batch test paper of a certain type
In the data field, reagent color blocks are attached for detecting different components of the sample: taking the example of the color blocks of the dry chemical test paper for detecting different components in urine, the detectable items include protein, glucose, ketone bodies, calcium, leucocytes, creatinine, occult blood, bilirubin, urobilinogen, nitrite, specific gravity, pH value, vitamin C, microalbumin, etc., and 14 reagent blocks are correspondingly required. When the arrangement order and position of the 14 reagent patches are defined, then the arrangement order and position are also referred to during the reading process.
When the urine test paper made of the test paper is put into urine or the urine is dripped on the urine, corresponding color change can be generated, and an interpretation result can be obtained by scanning the two-dimensional code pattern of the test paper. Such as the reagent block in which the Protein (PRO) is detected: PH indicator protein error principle. The reagent block contains bromophenol blue, a citric acid buffer system and a surfactant, wherein the pH threshold of the bromophenol blue is 3.0-4.6, anions can be generated at the pH of 3.2, a color reaction can be generated after the bromophenol blue is combined with protein with cations, the reagent block is suitable for detecting albumin, weak positive is realized when the concentration of globulin is high, and an interpretation result can be obtained through color comparison. The process of color comparison can be implemented with reference to the prior art.
The test paper based on two-dimensional coding positioning provided by the embodiment of the invention can determine the coordinate axis and coordinate scale of a test paper block by setting the characteristic pattern, can determine the position of each color block, can describe the characteristics of the test paper block including but not limited to the model number, the version number, the batch number and the like of the test paper by setting the characteristic code at a specific position, and can correct the chromatic aberration caused by different light sources by the set color correction block. Test paper blocks with different sizes can be placed at any position on the test paper substrate through the color block position table, and finally, a test result can be obtained through the judgment result table.
Because two tables are adopted to represent the detection method and the result of test paper blocks with different models, when a test paper block with a new model is developed, software of a detection instrument is not required to be modified, and the two tables can be downloaded on the OTA network, so that one machine has multiple purposes, and the upgrading and optimization can be realized at any time.
Example 2:
the difference from the above example 1 is: the chemical reagent blocks 3 are located outside the two-dimensional code area, as shown in fig. 2, the two-dimensional code is located at the top of the test paper, and the plurality of chemical reagent blocks 3 are located at the bottom of the test paper; the origin and the scale of the coordinate system of the test paper block are determined by scanning the two-dimensional code, then the positions of the chemical reagent blocks 3 below are determined, and the interpretation result of the detection sample is obtained by reading the RGB values of the corresponding positions.
Example 3:
as shown in fig. 3, the positioning test paper based on the linear stacked two-dimensional code is rectangular, and is relatively simple and clear, and only has a positioning pattern and a synchronization pattern. Two adjacent sides of the rectangle are formed by black lines and are positioning patterns; the other two adjacent sides of the rectangle are formed by black and white alternate straight lines and are synchronous patterns; the inside of the rectangle is provided with a plurality of test paper color blocks.
Example 4:
reading the test strip of any one of examples 1-3 above; referring to fig. 4, the interpretation method includes:
s10, obtaining a whole image of the positioning test paper based on the two-dimensional code;
for example, a color image of the whole test paper picture can be acquired by a CCD camera, wherein the two-dimensional code is identified by a relevant positioning pattern, a synchronization pattern and an alignment pattern (as shown in fig. 1 and 3) or a standard two-dimensional code area (as shown in fig. 2) for interpretation in step S20; for color recognition, each chemical reagent patch on the test strip has digital information in RGB color space for interpretation in step S30.
S20, identifying the two-dimension code information of the image through a two-dimension code identification terminal; in the step, the two-dimension code picture can be identified or the two-dimension code scanning terminal can directly read.
S30, reading RGB values of the chemical reagent blocks according to the two-dimensional code information; in the step, the positioning pattern and the synchronization pattern are recognized according to S20, coordinates of each chemical reagent block in the image are accurately obtained through coordinate positioning and segmentation, and then RGB values of the chemical reagent blocks are read.
And S40, comparing the RGB values with a judgment result threshold value table, and outputting an interpretation result of the test paper.
The comparison process, as shown in fig. 5, first introduces a three-dimensional rectangular coordinate system, and the three coordinate axes represent R, G, B numbers respectively. Since this value is limited to a maximum of 255, the value range forms a cube in the spatial space. The color values in any of the RGB color systems correspond to a point within the cube.
And (3) interpretation process: the spatial distances of the three-dimensional vectors between the reagent patches with a certain sequence number and the threshold values are calculated, and as shown in table 2, the RGB information of the current test strip patch is (R, G, B), and the RGB information of the threshold value is (R)ij,Gij,Bij)(1<i<N, N is the number of color blocks, 1<j<m, m are threshold numbers, which are respectively the first and the fourth columns in the table 2);
wherein, the distance of the color space is calculated as follows:
Li＝Min{L1，L2,....Lm}
on the principle that the closer the distance, the closer the color, i.e. LiThe smallest of these is the result of the interpretation. The result corresponding to the threshold (see table 2) is the final interpretation result. The same applies to test paper blocks with other serial numbers.
For example: for example, the RGB information obtained when the serial number of the color block of the current test paper is 1 is (R, G, B), and the RGB information of the threshold value is respectively: (R)11，G11，B11)、(R12，G12，B12)、(R13，G13，B13)、(R14，G14，B14) I.e. there are 4 thresholds. Then, the color space distances between (R, G, B) and the 4 thresholds are judged, and the 'result' corresponding to the minimum color space distance is selected as the final judgment result.
And the color can be converted into other color modes, such as HSL and HSV color modes for interpretation. For example, when the test paper of embodiment 1 is read, when the two-dimensional code is a matrix-type two-dimensional code, in S20, the two-dimensional code information of the image is identified through the two-dimensional code identification terminal, and the positioning pattern, the synchronization pattern, the alignment pattern, the white balance block, the format data pattern, and the chemical reagent block are identified; after the test paper model or the test paper batch number corresponding to the format data pattern is identified, searching a corresponding judgment result threshold value table; and when the two-dimensional code scanning terminal does not have the judgment result threshold value table, the corresponding judgment result threshold value table can be downloaded through the OTA.
For another example: when the two-dimensional code is a linearly stacked two-dimensional code, S20 may identify the positioning pattern, the synchronization pattern, and the chemical reagent patch.
According to the test paper interpretation method based on two-dimensional code positioning, provided by the embodiment of the invention, the characteristic pattern is identified by scanning the two-dimensional code, and the position of a coordinate axis is calculated; the image file is transformed by rotation, translation, alignment and the like, and the image file is normalized; then, the ID format code is identified, whether a model test paper block judgment result threshold table exists in the two-dimensional code identification terminal or not is judged, and if not, the corresponding judgment result threshold table is downloaded; finally, extracting color block color data according to the position and the size specified by the color block position table, solving the color space coordinate of each color block according to the existing algorithm, and solving the inspection result according to the judgment result threshold value table; and finally sorting the result, and outputting the final inspection result according to the agreed format.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (8)
1. The test paper based on two-dimensional coding location is characterized by comprising:
a test paper substrate;
the test paper substrate is provided with a positioning structure functional pattern based on two-dimensional codes, and at least one chemical reagent block for detecting a sample is arranged.
2. The test paper based on two-dimensional code positioning of claim 1, wherein when the two-dimensional code is a matrix two-dimensional code, the positioning structure function pattern comprises: a registration pattern, a synchronization pattern, an alignment pattern, and a white balance weight.
3. The test paper based on two-dimensional coding positioning as claimed in claim 2, characterized in that a format data pattern is further provided on the test paper substrate; the format data pattern is used for storing at least one of the following information:
test paper model, test paper batch number or error correction code.
4. The test paper based on two-dimensional code positioning of claim 1, wherein when the two-dimensional code is a linear stacked two-dimensional code, the positioning structure function pattern comprises: a positioning pattern and a synchronization pattern.
5. The test paper based on two-dimensional code positioning as claimed in claim 1, characterized in that the chemical reagent block is located in the internal data field of the two-dimensional code; or the chemical reagent blocks are arranged in the region outside the two-dimensional code according to a preset rule.
6. A method for interpreting a test strip positioned based on two-dimensional codes, which is characterized by interpreting the test strip of any one of claims 1-5; the interpretation method comprises the following steps:
obtaining a whole image based on two-dimensional coding positioning test paper;
identifying two-dimension code information of the image through a two-dimension code identification terminal;
reading RGB values of the chemical reagent blocks according to the two-dimension code information;
and comparing the RGB numerical values with a corresponding judgment result threshold value table, and outputting an interpretation result of the test paper.
7. The interpretation method of claim 6, wherein identifying the two-dimensional code information of the scanned image comprises:
when the two-dimensional code is a matrix two-dimensional code, identifying a positioning pattern, a synchronous pattern, an alignment pattern, a white balance block, a format data pattern and a chemical reagent block;
when the two-dimensional code is a linear stacked two-dimensional code, a positioning pattern, a synchronization pattern, and a chemical reagent patch are identified.
8. The interpretation method of test paper based on two-dimensional coding positioning according to claim 7, further comprising:
after the test paper model or the test paper batch number corresponding to the format data pattern is identified, searching a corresponding judgment result threshold value table; and when the two-dimensional code scanning terminal does not have the judgment result threshold value table, downloading the corresponding judgment result threshold value table through an OTA.
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