TWI712692B - The extraction method of Abel fruit extract used to regulate the metabolism of the digestive system in the body - Google Patents

Abstract

The present invention relates to an extraction method of Abel fruit extract used to regulate the metabolism of the digestive system in the body. It also relates to a mixture of Abel fruit extracts. The method mainly first weighs 10 grams of Abel fruit powder and puts it in 100 One milliliter of distilled water was once again immersed in deionized pure water for a period of time, and vacuum extraction was carried out in an environment of 30-50 degrees Celsius. After cooling, 228 grams of protease was added to maintain the temperature at 37 degrees Celsius. After acting for a period of time, the enzyme reaction is stopped by heating to obtain comprehensive plant enzymes such as mono- and dianthracene compounds. Then filter the above-mentioned liquid after the protease reaction is completed to filter out undissolved impurities, and the filtrate is concentrated under reduced pressure and then heat sterilized by the pasteurizing method. After heat sterilization, it is rapidly cooled for secondary sterilization. At the time, the liquid Able fruit extract was obtained, and the liquid Able fruit extract was stored at 4 degrees Celsius for a specific time. The aforementioned liquid Able fruit extract is mixed with 0-10% by weight of excipients and freeze-dried to obtain a powdered Able fruit extract, which is then applied to the powdered Able Appropriate amount of edible materials are added to the fruit extract to prepare soft sweets, tea bags or foods containing the fruit extract of Abel, which can be used to prevent and/or resist carbohydrates and/or in humans or animals. The purpose of lipodystrophy.

Description

The extraction method of Abel fruit extract used to regulate the metabolism of the digestive system in the body

The present invention relates to an extraction method of Abel fruit extract used to regulate the metabolism of the digestive system in the body. It also relates to a mixture of Abel fruit extracts, especially one that can be used to prevent and/or resist in humans or animals. Abele fruit extracts and mixtures of carbohydrates and/or fat metabolism disorders can prevent and reduce functional constipation.

With the influence of people's diet, mental and psychological, and social factors, the incidence of constipation tends to increase. Constipation can affect people of all ages. Among them, there are more women than men, and more elderly than young and middle-aged people. The prevalence rate in the population is as high as 27%. It increases with age, reaching 28-50% after 60 years of age. Moreover, many patients have less than three bowel movements per week, and in severe cases, they only defecate once for 2-4 weeks.

People with frequent constipation, such as long-term use of laxatives, will cause drug dependence, and will not be able to defecate without laxatives. Drugs can only relieve temporary difficulties, but it is difficult to eradicate them for a long time. There are countless herbal diets in Chinese medicine, and dietary conditioning is also effective for constipation. Such as cassia seed porridge, shouwu porridge, mulberry porridge, sweet potato porridge, etc. However, eating these foods requires time-consuming cooking and also requires moderate consumption. However, because ordinary people cannot accurately understand the amount of food needed when eating, they often cause excessive amounts. side effect.

Abel (Cassia fistula) is a very common plant in India, also known as golden shower. It has laxative properties. In Ayurvedic medicine, it is used to combat various diseases, such as hematemesis, itching, leukoplakia and diabetes, antifungal and antibacterial activities. The fruit also has antioxidant properties, and it is also often used in the Chinese Materia Medica. Used to cure diseases. According to traditional Chinese medicine, the root taste is bitter and cool in nature. It is used for astringent, strong, antipyretic, diarrhea, and also for heartache. The leaves are used for skin diseases, ringworm, rheumatism, and stroke. The flower is used for constipation and as an antipyretic. The fruit is bitter and cold in nature. It has the functions of controlling fever, eliminating phlegm, killing insects, and clearing meridians. It is used for bone steaming, tuberculosis fever, gastritis (stomach, duodenum, acute and chronic gastritis, gastrointestinal neurosis, etc.), and also improves constipation, hyperacidity, and loss of appetite. This product is also an anti-pain and laxative, but after a long decoction (more than 8 hours), it has no diarrhea effect, but has astringent effect.

<The technical problem to be solved> In view of the above-mentioned people's long-term dependence on drugs to solve the difficulties of digestive system constipation and the defects to be improved, the inventor hopes to provide a breakthrough food to enhance the practical effect. After testing, integrating his years of professional technical knowledge and practical experience in related product design, production and marketing, and the results of research and design, he finally developed an extraction method of Able fruit extract to regulate the metabolism of the digestive system in the body. To prevent and reduce functional constipation.

<Comparing the effects of the prior art> The main purpose of the method of the present invention is to make edible food by extracting the extracts from the fruit of Abel to prevent and reduce functional constipation.

The second purpose of the method of the present invention is to make edible foods by extracting the extracts from the Arbel fruit, which is convenient to eat and carry, does not require cooking, and does not cause side effects.

In order to enable your reviewers to better understand the features and efficacy of the method of the present invention, the figures are combined and detailed in the following.

Please refer to the first figure. The present invention relates to an extraction method of Able fruit extracts used to regulate the metabolism of the digestive system in the body. It also relates to a mixture of Able fruit extracts. After washing and draining (11), drying (12), grinding (13) and other steps, the fruit is made into a crude extract of Able fruit powder (14), and then weigh 10 grams of Able fruit powder (14) and place it at 100 One milliliter of distilled water is then immersed in deionized pure water (15) for 0.5 to 1 hour, and then subjected to vacuum decompression extraction (16), that is, vacuum is drawn in an environment of 30 to 50 degrees Celsius (pressure For 10~200 Torr (torr)) for 30 minutes to 6 hours. After cooling (17) to 37 degrees Celsius, add 228 grams of protease (18) (which can be papain, trypsin, or bromelain), keep it at 37 degrees Celsius for 1-12 hours, then stop the enzyme by heating React (19) to obtain comprehensive plant enzymes such as mono- and dianthracene compounds (20). Then filter (21) the above-mentioned liquid after the protease reaction is completed to filter out undissolved impurities, and concentrate the filtrate under reduced pressure (22) and heat it to 65-75 degrees Celsius for sterilization by pasteurizing (23) , And maintain this temperature for 10-50 minutes and then rapidly cool (24) to 4-7 degrees Celsius for the second sterilization, obtain the liquid Abel fruit extract (25), this liquid Abel fruit extract (25) The solution will be stored at 4 degrees Celsius and will only be stored for 3-10 days, up to 16 days. The aforementioned liquid Able fruit extract (25) is mixed with 0-10% by weight of excipients (26), and then freeze-dried (27) to obtain a powdered Able fruit extract (28), and The optimal ratio of the aforementioned Abele fruit powder (14), pure water (15) and protease (18) is 1:10:22.8.

The aforementioned drying (12) can be carried out in the sun or by drying, the aforementioned excipient (26) can be starch, corn starch, maltodextrin, lactose, dextrin, or cellulose, and the aforementioned protease (18) can be It is papain, trypsin, or bromelain. In addition, 228 grams of protease (18) was added to carry out the enzymatic reaction. Enzymatic reaction (also known as enzyme catalysis or enzymatic reaction) refers to a chemical reaction catalyzed by a special kind of protein called enzyme. Because the rate of non-catalytic reactions is extremely slow, the catalysis of biochemical reactions in cells is extremely important. The mechanism of enzymatic reaction is similar in principle to other types of chemical catalysis. Enzymes reduce the energy requirement to reach the highest energy transition state by providing alternative reaction routes and methods to stabilize intermediate products. The decrease in activation energy (Ea) increases the number of reactant molecules that have enough to reach activation energy and form products.

In the present invention, the powdered Abel fruit extract (28) obtained above is added with an appropriate amount of water, gelatin, glucose, sucrose, citric acid, and appropriate flavors and sweeteners at a weight percentage of 0.02%-3%. A soft candy containing Abel fruit extract is prepared. Or add appropriate amount of sugar, spices and sweeteners to make tea bags containing Abel fruit extract.

The following are the experimental data of the Abel fruit extract obtained in the present invention. The effects are as described above, and are explained as follows:

Experimental example 1, as shown in the second figure, is a comparison between the Abel fruit extract extracted by the method of the present invention and a commercially available laxative to compare whether to increase the number of stools in rats. The system uses 20kg/ml of Arbol fruit extract and 20kg/ml of laxative to feed the rats once a day, and then filter the rat feces with a sieve every two days to calculate the number of feces and continue On the 14th, it can effectively increase the number of stools in rats. The results are expressed as mean ± standard deviation.

Experimental example two, as shown in the third figure, is an experiment to compare whether the Able fruit extract extracted by the method of the present invention and the commercially available laxatives increase the weight of rat feces. 20 kg/ml of Arbol fruit extract and 20 kg/ml of laxative were given to rats by feeding once a day, and then the stool weight was weighed every two days for 14 days. It can effectively increase the weight of rat feces, and the results are expressed as mean ± standard deviation.

Experimental example three, as shown in the fourth figure, is a soft candy made from the Abel fruit extract extracted by the method of the present invention to test the consumption of testers of different ages to promote defecation. The Able fruit extract is made into soft candies and given to people aged 10-20, 20-30, 30-40, 40-50 once a day, and count the daily consumption that can promote defecation After that, it is converted into daily consumption, and the statistical time is 3 weeks. Furthermore, the consumption data of soft candies made from the Abel fruit extract that promotes defecation for testers of different ages was obtained, and the results were expressed as mean±standard deviation.

Experimental Example 4, as shown in the fifth figure, was made into soft candy from the Abel fruit extract extracted by the method of the present invention to effectively improve bowel pain for testers of different ages. The Able fruit extract is made into soft candies, which are given once a day to people aged 10-20, 20-30, 30-40, and 40-50 (n=50) in questionnaires Count the degree of somatosensory sensation of defecation pain, with 100 as the highest and 0 as the lowest. The statistical time is three weeks. It can be clearly seen that after three weeks of consumption, it can effectively improve the body sensation of bowel pain, and the results are expressed as an average value.

Experimental example five, as shown in the sixth figure, is an experiment conducted by using the Able fruit extract extracted by the method of the present invention to effectively reduce the hardness of feces in different age groups. The Able extract fruit extracts are made into soft sweets, which are given to 10-20 years old, 20-30 years old, 30-40 years old, and 40-50 years old people (n=50) in a questionnaire once a day. Count the degree of fecal hardness and somatosensory, with 100 as the highest and 0 as the lowest. The statistical time is three weeks. It can be clearly seen that after three weeks of consumption, it can effectively reduce the degree of fecal hardness and somatosensory, and the result is expressed as an average value.

In summary, the extraction method of the Abel fruit extract used in the present invention to regulate the metabolism of the digestive system in the body. The extract is processed into food and after consumption, it can effectively increase the amount of defecation, reduce the degree of painful defecation and The fecal hardness and somatosensory effect is a very novel, progressive, and industrially applicable invention. It has actually met the requirements for invention patents. A patent application is filed in accordance with the law. I hope that the examiner can examine it in detail. , And granting the patent for this case as soon as possible is indeed a virtue.

Only the above are only the preferred embodiments of the present invention. When they cannot be used to limit the scope of the present invention, all equivalent changes and modifications made in accordance with the scope of the patent application of the present invention shall still belong to the patent of the present invention. Covered.

(11)‧‧‧Cleaning and draining (12)‧‧‧Drying (13)‧‧‧Grinding (14)‧‧‧Able fruit powder (15)‧‧‧Pure water (16)‧‧‧Vacuum reduction Pressure extraction (17) ‧ ‧ cooling (18) ‧ ‧ protease (19) ‧ ‧ heating to stop the enzyme reaction (20) ‧ ‧ obtaining mono- and dianthracene compounds and other comprehensive plant enzymes (21) ‧ ‧ filtering (22)‧‧‧Concentration under reduced pressure (23)‧‧‧Low-temperature sterilization (24)‧‧‧Quick cooling (25)‧‧‧Liquid Abel Fruit Extract (26)‧‧‧Excipients (27) ‧‧‧Freeze drying process (28)‧‧‧Powdered Abel Fruit Extract

The first figure is the flow block diagram of the extraction method of Abel fruit extract of the present invention. The second figure is an experiment comparing whether the Abele fruit extract extracted by the method of the present invention and the commercially available laxative increase the number of stools in rats. The third figure is an experiment comparing whether the Abel fruit extract extracted by the method of the present invention and the commercially available laxative increase the weight of rat feces. The fourth figure is a graph showing the consumption data of soft candies made from the Abel fruit extract extracted by the method of the present invention to promote defecation in testers of different ages. The fifth graph is a data graph showing how soft sweets made from the Abel fruit extract extracted by the method of the present invention can effectively improve the somatosensory sensation of defecation pain for testers of different ages. The sixth figure is a data diagram of experiments conducted on soft candies made from the Abel fruit extract extracted by the method of the present invention to effectively reduce the degree of fecal hardness and somatosensory in testers of different ages.

(11)‧‧‧Wash and drain

(12)‧‧‧Drying

(13)‧‧‧Grinding

(14)‧‧‧Able Fruit Powder

(15)‧‧‧Pure water

(16)‧‧‧Vacuum decompression extraction

(17)‧‧‧Cooling

(18)‧‧‧Protease

(19)‧‧‧Heat to stop the enzyme reaction

(20)‧‧‧Obtained comprehensive plant enzymes such as mono- and dianthracene compounds

(21)‧‧‧Filter

(22)‧‧‧Decompression Concentration

(23)‧‧‧Low temperature sterilization

(24)‧‧‧Quick cooling

(25)‧‧‧Liquid Abel Fruit Extract

(26)‧‧‧Excipients

(27)‧‧‧Perform freeze-drying treatment

(28)‧‧‧Powdered Abel Fruit Extract

Claims (10)

A method for extracting the Abele fruit extract to regulate the metabolism of the digestive system in the body. The main method is as follows: First, the Abele fruit is washed, drained, dried, and ground into a crude extract of Abele fruit powder. , Weigh 10 grams of Abele fruit powder and place it in 100 milliliters of distilled water and then soak in deionized pure water for 0.5 to 1 hour. After vacuum extraction, add 228 grams of protease Carry out the enzyme reaction, and then stop the enzyme reaction by heating to obtain a comprehensive plant enzyme liquid such as mono- and dianthracene compounds, and then filter the comprehensive plant enzyme liquid obtained after the protease reaction is completed to filter out undissolved impurities. The filtrate of the integrated plant enzyme liquid obtained after the filtration of the protease reaction is completed under reduced pressure and concentrated, then heated by the Baroque low-temperature sterilization method for sterilization, and maintained at this temperature for a period of time, and then rapidly cooled for the second sterilization to obtain the liquid Abel Fruit extract. This liquid Able fruit extract will be stored in a low temperature state. The liquid Able fruit extract is mixed with 0-10% by weight of excipients for freeze-drying to obtain a powdered Able Boehler fruit extract, and the optimal ratio of the aforementioned Boehler fruit powder, pure water and protease is 1:10:22.8. As described in item 1 of the scope of patent application, the extraction method of Abel fruit extract used to regulate the metabolism of the digestive system in the body, in which the step of vacuum decompression extraction is to draw a vacuum in an environment of 30-50 degrees Celsius with a pressure of 10 ~200 Torr (torr) for 30 minutes to 6 hours, after cooling to 37 degrees Celsius, then add 228 grams of protease. As described in the first item of the scope of patent application, the extraction method of Abel fruit extract for regulating the metabolism of the digestive system in the body, wherein the protease can be papain, trypsin or bromelain. As described in item 1 of the scope of patent application, the extraction method of Abel fruit extract used to regulate the metabolism of the digestive system in the body, wherein a protease is added to carry out an enzymatic reaction, and the enzymatic reaction is maintained at 37 degrees Celsius for 1-12 hours. As described in the first item of the scope of patent application, the extraction method of Abel fruit extract used to regulate the metabolism of the digestive system in the body, wherein the pasteurization method is heated to 65 to 75 degrees Celsius for sterilization, and the temperature is maintained for 10~ After 50 minutes, rapid cooling is performed. As described in the first item of the scope of patent application, the extraction method of Abel fruit extract used to regulate the metabolism of the digestive system in the body, wherein the temperature of the pasteurized heat sterilization followed by rapid cooling is 4-7 degrees Celsius. As described in the first item of the scope of patent application, the extraction method of Abel fruit extract used to regulate the metabolism of the digestive system in the body, wherein the excipient can be starch, corn starch, maltodextrin, lactose, dextrin or fiber Vegetarian. As described in the first item of the scope of patent application, the extraction method of Able fruit extract used to regulate the metabolism of the digestive system in the body, wherein the liquid Able fruit extract obtained after the second sterilization will be stored in Celsius Store in a low temperature of 4 degrees for only 3 to 10 days, up to 16 days. As described in the first item of the scope of patent application, the extraction method of Able fruit extract used to regulate the metabolism of the digestive system in the body, wherein the obtained powdered Able fruit extract is added at a weight percentage of 0.02%-3% Appropriate amount of water, gelatin, glucose, sucrose, citric acid, and appropriate flavors and sweeteners to prepare soft candies containing Abel fruit extract. As described in the first item of the scope of patent application, the extraction method of Able fruit extract to regulate the metabolism of the digestive system in the body, wherein the obtained powdered Able fruit extract is added with appropriate amounts of sugar, spices and sweetness To prepare a tea bag containing Abel fruit extract.

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