TWI703326B - 藉由測定精子獲能以鑑定雄性生殖力狀態 - Google Patents
藉由測定精子獲能以鑑定雄性生殖力狀態 Download PDFInfo
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- TWI703326B TWI703326B TW104130832A TW104130832A TWI703326B TW I703326 B TWI703326 B TW I703326B TW 104130832 A TW104130832 A TW 104130832A TW 104130832 A TW104130832 A TW 104130832A TW I703326 B TWI703326 B TW I703326B
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
- G01N2800/367—Infertility, e.g. sperm disorder, ovulatory dysfunction
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Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462051536P | 2014-09-17 | 2014-09-17 | |
| US201462051533P | 2014-09-17 | 2014-09-17 | |
| US62/051,536 | 2014-09-17 | ||
| US62/051,533 | 2014-09-17 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW201629488A TW201629488A (zh) | 2016-08-16 |
| TWI703326B true TWI703326B (zh) | 2020-09-01 |
Family
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Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
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| TW104130832A TWI703326B (zh) | 2014-09-17 | 2015-09-17 | 藉由測定精子獲能以鑑定雄性生殖力狀態 |
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| TW109126397A TWI777212B (zh) | 2014-09-17 | 2015-09-17 | 藉由測定精子獲能以鑑定雄性生殖力狀態 |
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| TWI777212B (zh) * | 2014-09-17 | 2022-09-11 | 美國康奈爾大學 | 藉由測定精子獲能以鑑定雄性生殖力狀態 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2016379379B2 (en) * | 2015-12-23 | 2022-12-15 | Androvia Life Sciences LLC | A method to identify an approach for achieving mammalian fertilization and time period for insemination |
| JP6941616B2 (ja) * | 2016-02-17 | 2021-09-29 | アンドロヴィア ライフサイエンシーズ， エルエルシー | 男性の受精能状態を決定するための方法および試験キット |
| EP3873363A4 (en) * | 2018-11-02 | 2022-11-23 | Androvia Lifesciences, Llc | IDENTIFICATION OF MALE FERTILITY STATUS BY DETERMINATION OF SPERM CAPACITY AND COMPANION COLLECTION KIT |
| WO2020223515A1 (en) * | 2019-04-30 | 2020-11-05 | Androvia Lifesciences, Llc | Use of cap-score tm in identification of a reproductive approach in men suffering from varicocele |
| CN113237942B (zh) * | 2021-05-07 | 2023-09-26 | 上海科技大学 | 在微量细胞中检测多种微量元素的方法及应用 |
| TW202311743A (zh) | 2021-07-26 | 2023-03-16 | 日商電化股份有限公司 | 包含人類化抗體之試劑 |
| AU2024303117A1 (en) * | 2023-06-12 | 2026-01-15 | Colossal Biosciences Inc. | Laser-assisted in vitro fertilization |
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| US20100248302A1 (en) * | 2003-07-23 | 2010-09-30 | Travis Alexander J | Method of Determining Sperm Capacitation |
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| JP2651249B2 (ja) * | 1989-09-27 | 1997-09-10 | 扶桑薬品工業 株式会社 | 精子の受精能試験具および試験法 |
| WO1998000440A1 (en) | 1996-07-03 | 1998-01-08 | Zygam, Inc. | Immunocontraceptive compositions containing sperm antigens, and methods of use |
| NZ506887A (en) | 1998-03-30 | 2003-12-19 | Bioshaf Ltd | Flow cytometer analysis of cells and body fluids for fertility testing |
| CA2533542C (en) | 2003-07-23 | 2013-06-11 | Cornell Research Foundation, Inc. | Method of determining sperm capacitation |
| WO2009131654A2 (en) * | 2008-04-21 | 2009-10-29 | North Carolina State University | Inhibition and dispersion of bacterial biofilms with imidazole-triazole derivatives |
| US9759728B2 (en) | 2013-09-05 | 2017-09-12 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Methods for determining human sperm quality |
| WO2016044392A1 (en) * | 2014-09-17 | 2016-03-24 | Cornell University | Identifying status of male fertility by determining sperm capacitation |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100248302A1 (en) * | 2003-07-23 | 2010-09-30 | Travis Alexander J | Method of Determining Sperm Capacitation |
Non-Patent Citations (2)
| Title |
|---|
| Neri, Q. V., et al. "Testing the effect of cryopreservation on a biomarker-based assay of sperm function: toward generating a standard for semen samples with known fertility." Fertility and Sterility 100.3 (2013): S451. * |
| Vairo, L., et al. "A novel, biomarker-based assay to screen for dysfunctional spermatozoa." Fertility and Sterility 100.3 (2013): S224-S225. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI777212B (zh) * | 2014-09-17 | 2022-09-11 | 美國康奈爾大學 | 藉由測定精子獲能以鑑定雄性生殖力狀態 |
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