TWI701334B - Enzyme solution, enzyme-containing non-woven fabric, and methods of preparation and use thereof - Google Patents

Abstract

Disclosed is a enzyme solution comprising a nonionic surfactant, an enzyme, a normal-tempernature enzyme stabilizer, an isothiazolinone, a benzoisothiazolinone, sodium benzoate, a solubilizer, a pH adjuster, and water. In some embodiments, the enzyme solution also contains a chelating agent. The enzyme solution according to the present disclosure may be used to clean medical devices, and has good compatibility with non-woven fabrics comprising viscose fiber and terylene fiber. The enzyme solution dispersed in the non-woven fabric not only has good enzyme stability, but also has good mold-resistance and bacteria-resistance properties. Furthermore, the enzyme-containing non-woven fabric according to the present disclosure is less likely to cause damage to medical devices, and is less likely to cause corrosion to the metal or rubber components of the medical devices.

Description

Enzyme solution, enzyme-containing non-woven fabric, and preparation and use methods thereof [Cross-reference related applications]

This application claims the priority of Chinese Patent Application No. 201510023223.4 filed on January 16, 2015, the disclosure of which is incorporated herein by reference in its entirety.

This disclosure relates to the field of washing and disinfection, and particularly to enzyme solutions, enzyme-containing non-woven fabrics, and methods of preparation and use thereof.

Washing and disinfection (or disinfection) represents a key step in the treatment of medical devices after use. For heavily contaminated medical devices, pre-rinsing or pre-cleaning steps can significantly increase the success rate of subsequent washing and disinfection, extend the service life of medical devices, and reduce the risk of contamination. For example, in a hospital, after the doctor removes the endoscope from the patient’s body, he will immediately use a wet wipe or wet gauze containing a washing solution to wipe the contaminants on the outer surface of the endoscope. The processor completes pre-cleaning before removing the endoscope.

At present, most hospitals perform pre-cleaning of endoscopes by using traditional gauze soaked with a diluted enzyme solution. However, the diluted enzyme solution The enzymes in the product have relatively weak stability, which may lead to a decrease in the success rate of pre-cleaning. In addition, traditional gauze has relatively weak compatibility with enzyme solutions. After the enzyme solution is dispersed into the traditional gauze, the enzyme activity will rapidly decrease, which may also affect the success rate of the pre-cleaning.

It should be understood that various other embodiments can be envisaged, and those skilled in the art can modify the teachings of this patent specification without departing from the scope or spirit of this disclosure. Therefore, the following detailed description is not meant as a limitation.

Unless otherwise specified, all numbers used to indicate the size, quantity, and physical and chemical properties of features in this specification and the scope of the patent application should be understood as modified by the word "about" in all examples. Therefore, unless otherwise indicated to the contrary, the numerical parameters set forth in the foregoing specification and the appended patent scope are approximate values, which can be based on the desired characteristics obtained by those skilled in the art using the teachings disclosed herein. And after appropriate modification. The numerical range expressed by the endpoints includes all numbers included in the range and any range within the range. For example, 1 to 5 include 1, 1.1, 1.3, 1.5, 2, 2.75, 3, 3.80, 4, 5, and so on.

Enzyme solution

In some aspects, the present disclosure provides an enzyme solution, based on the total weight of the enzyme solution, which contains 0.1 to 10 wt.% of nonionic surfactant; 0.01 to 1.2 wt.% of enzyme; 0.03 to 15 wt.% of Normal temperature enzyme stabilizer; 0.75 to 6wt.% isothiazolinone; 0.1 to 1wt.% benzoisothiazolinone; 0.1 to 1wt.% sodium benzoate; 6.9 to 10wt.% Co-solvent; 0.15 to 0.5wt.% of pH adjuster; and 60 to 85wt.% of water; the enzyme solution has a pH of 6 to 10. In some aspects of the present disclosure, the enzyme solution contains 0.1 to 0.7 wt.% of sodium benzoate. In some aspects of the present disclosure, the enzyme solution contains 0.1 to 0.5 wt.% sodium benzoate. In some aspects of the present disclosure, the enzyme solution contains about 5 to 13% of the room temperature enzyme stabilizer. In some aspects, the enzyme solution of the present disclosure contains about 10 to 11% of the room temperature enzyme stabilizer.

Nonionic surfactant

In the enzyme solution according to the present disclosure, the non-ionic surfactant helps to reduce the surface tension of the enzyme solution and increase the wetting and cleansing ability of the enzyme solution.

Several suitable nonionic surfactants can be used in the enzyme solution of the present disclosure. According to some embodiments, the non-ionic surfactant comprises at least one component, which is selected from the group consisting of: (i) polymer, which is based on ethylene oxide, propylene oxide ), and at least one of butylene oxide; (ii) alkyl glucoside; (iii) fatty alcohol poly Fatty alcohol polyoxyethylene ether; and (iv) ammonium oxide.

According to certain embodiments, the polymer based on at least one of ethylene oxide, propylene oxide, and butylene oxide includes at least one component selected from ethylene oxide/butylene oxide copolymers and ethylene oxide/propylene oxide/ A group of butylene oxide copolymers. According to certain embodiments, EH-6 available from The Dow Chemical Company may be selected as the polymer based on at least one of ethylene oxide, propylene oxide, and butylene oxide.

According to certain embodiments, the alkyl glucoside has a molecular weight of 320 to 350. According to certain embodiments, the alkyl glucoside comprises at least one component selected from the group consisting of decyl glucoside, octyl glucoside, and dodecyl glucoside. Groups formed. According to certain embodiments, Glucopon 425N/HH available from BASF Corporation can be selected as the alkyl glucoside.

According to certain embodiments, the fatty alcohol polyoxyethylene ether has the general formula RO(CH ₂ CH ₂ O) _n H, where n=8-16. According to some embodiments, the fatty alcohol polyoxyethylene ether comprises at least one component, which is selected from the group consisting of: C7 to C9 fatty alcohol polyoxyethylene ether, C12 to C16 fatty alcohol polyoxyethylene ether, And C9 to C11 fatty alcohol polyoxyethylene ether. According to certain embodiments, A1058 available from Harcros Corporation can be selected as the fatty alcohol polyoxyethylene ether.

Based on the total weight of the enzyme solution, the amount of the nonionic surfactant is 0.1 to 10 wt.%. When the amount of the non-ionic surfactant is less than 0.1wt.% When the amount of the enzyme solution is greater than 10wt.%, the enzyme solution may suffer excessive foaming, resulting in a reduced cleaning ability of the enzyme solution.

Enzyme

In the enzyme solution according to the present disclosure, the enzyme helps decompose proteins, sugars, fats, and cellulose in contaminants associated with medical devices.

According to some embodiments, the enzyme includes at least one component selected from the group consisting of proteolytic enzymes, amylolytic enzymes, lipolytic enzymes, and cellulolytic enzymes.

According to certain embodiments, Alcalase, Esperase, Everlase, Savinase 16XL, Liquanase, or Polarzyme available from Novozymes Corporation can be selected as the proteolytic enzyme. According to some embodiments, Properase available from Genencor Corporation can also be selected as the proteolytic enzyme.

According to certain embodiments, Stainzyme, Termamyl 300L, BAN 480L, or Duramyl available from Novozymes Corporation can be selected as the amylolytic enzyme.

According to certain embodiments, Lipolase available from Novozymes Corporation or Purafect available from Genencor Corporation can be selected as the lipolytic enzyme.

According to certain embodiments, Carezyme 4500 or Endolase available from Novozymes Corporation can be selected as the cellulolytic enzyme.

Based on the total weight of the enzyme solution, the amount of the enzyme is 0.01 to 1.2 wt.%. According to some embodiments, based on the total weight of the enzyme solution, the enzyme in the enzyme solution contains 0.05 to 0.6 wt.% of proteolytic enzyme, 0.01 to 0.2 wt.% of amylolytic enzyme, and 0.01 to 0.2 wt.% The lipolytic enzyme, and 0.01 to 0.2wt.% cellulolytic enzyme. When the amount of the enzyme is less than 0.01 wt.%, the cleansing ability of the enzyme solution may be reduced; and when the amount of the enzyme is more than 1.2 wt.%, the stability of the enzyme solution may be reduced.

Room temperature enzyme stabilizer

In the enzyme solution according to the present disclosure, the room temperature enzyme stabilizer helps maintain the activity of the enzyme at room temperature (10 to 25°C).

Several normal temperature enzyme stabilizers may be suitable for use in the enzyme solution according to the present disclosure. In some embodiments, the room temperature enzyme stabilizer includes at least one component selected from the group consisting of glycerin, propylene glycol, calcium chloride, magnesium chloride, and boric acid.

According to certain embodiments, the glycerin can be purchased from The Dow Chemical Company.

According to some embodiments, the propylene glycol, calcium chloride, magnesium chloride, and boric acid can all be purchased from Sinopharm Chemical Reagent Co., Ltd., China.

Based on the total weight of the enzyme solution, the room temperature enzyme stabilizer is present in an amount of 0.03 to 15 wt.%. According to some embodiments, based on the total weight of the enzyme solution, the room temperature enzyme stabilizer in the enzyme solution includes 5 to 10 wt.% of glycerin and/or propylene glycol, and 0.03 to 0.7 wt.% of calcium chloride and/ Or magnesium chloride. When the amount of the normal temperature enzyme stabilizer is less than 0.03wt.%, the stability of the enzyme in the enzyme solution under normal temperature (10 to 25°C) conditions may be reduced; and when the amount of the normal temperature enzyme stabilizer is more than 15wt. .%, the cleaning ability of the enzyme solution can be reduced.

Isothiazolinone, benzisothiazolinone, and sodium benzoate

In the enzyme solution according to the present disclosure, isothiazolinone, benzisothiazolinone, and sodium benzoate are used in combination. Including isothiazolinone, benzisothiazolinone, and sodium benzoate in the enzyme solution not only helps maintain the good stability of the enzyme in the enzyme solution under relatively high temperature (above 25°C) conditions, It also contributes to the good anti-fungal and anti-bacterial properties of the enzyme solution and the good compatibility of the enzyme solution with non-woven fabrics.

According to some embodiments, the isothiazolinone comprises at least one component selected from the group consisting of 5-chloro-2-methyl-4-isothiazolin-3-one (5-chloro-2-methyl-4- isothiazolin-3-one (CMI) and 2-methyl-4-isothiazolin-3-one (MI). According to certain embodiments, Nipagurad CG available from Clariant Corporation may be selected as the isothiazolinone.

According to certain embodiments, the benzisothiazolinone comprises 1,2-benzoisothiazolin-3-one (1,2-benzoisothiazolin-3-one). According to certain embodiments, BIT (Prexel GXL) available from Lonza Corporation can be selected as the isothiazolinone.

According to certain embodiments, the sodium benzoate has a molecular formula of C ₆ H ₅ CO ₂ Na. According to certain embodiments, the sodium benzoate can be purchased from Shanghai Shantou Guanghua Chemical Reagents Co., Ltd.

Based on the total weight of the enzyme solution, the enzyme solution includes 0.75 to 6 wt.% of isothiazolinone, 0.1 to 1 wt.% of benzisothiazolinone, and 0.1 to 1 wt.% of sodium benzoate. When the amount of any one of the isothiazolinone, the benzisothiazolinone, and the sodium benzoate is inconsistent with the above-mentioned range, the enzyme in the enzyme solution is heated at a relatively high temperature (above 25°C). The stability can be reduced, the antifungal or antibacterial properties of the enzyme solution can be reduced, and the compatibility of the enzyme solution with the non-woven fabric can also be reduced.

According to some embodiments, when the weight ratio of the isothiazolinone and the benzisothiazolinone is 3:1 to 30:1, and the weight ratio of the isothiazolinone and the sodium benzoate is 1:1 to At 30:1, the enzyme in the enzyme solution has particularly good stability under relatively high temperature (above 25°C) conditions, and the enzyme solution has particularly good antifungal and antibacterial properties.

Cosolvent

In the enzyme solution according to the present disclosure, the co-solvent helps to promote the dissolution of the components in the enzyme solution.

Several cosolvents are suitable for use in the enzyme solution of the present disclosure. According to some embodiments, the co-solvent includes at least one component selected from the group consisting of: xylene sulfonic acid, sodium xylene sulfonate, xylene sulfonic acid (xylene sulfuric acid), sodium xylene sulfate (sodium xylene sulfate), and alkyl amine oxide (alkyl amine oxide).

According to certain embodiments, SXS 93 available from Kuantum Corporation can be selected as the sodium xylene sulfonate.

Based on the total weight of the enzyme solution, the co-solvent is present in an amount of 6.9 to 10 wt.%. When the co-solvent is present in an amount less than 6.9 wt.%, some components in the enzyme solution may not be fully dissolved; and when the co-solvent is more than 10 wt.%, the activity of the enzyme in the enzyme solution Can be lowered.

pH adjuster

In the enzyme solution according to the present disclosure, the pH adjusting agent is used to adjust the pH value of the enzyme solution.

Several pH adjusting agents can be suitably used in the enzyme solution of the present disclosure. According to certain embodiments, the pH adjusting agent comprises citric acid.

According to certain embodiments, the citric acid can be purchased from Sinopharm Chemical Reagent Co., Ltd., China.

Based on the total weight of the enzyme solution, the pH adjusting agent is present in an amount of 0.15 to 0.5 wt.%. When the pH adjuster is present in an amount outside the above range, The enzyme solution may become too acidic (pH value less than 6) or too alkaline (pH value greater than 10), resulting in a decrease in the activity of the enzyme in the enzyme solution.

According to some embodiments, the pH of the enzyme solution is in the range of 6-10. According to some embodiments, the pH of the enzyme solution is in the range of 7-9.

water

In the enzyme solution according to the present disclosure, water is used as a solvent.

According to certain embodiments, the water system is present in an amount of at least 60 wt.% based on the total weight of the enzyme solution. According to some embodiments, based on the total weight of the enzyme solution, the water system is present in an amount not greater than 85%. When water is present in an amount of less than 60wt.%, some components in the enzyme solution may not be fully dissolved, leading to the precipitation of some undesired components. When water is present in an amount of more than 85 wt.%, the components in the enzyme solution may be excessively diluted, thereby reducing the cleansing ability of the enzyme solution.

Chelating agent

Based on the total weight of the enzyme solution, the enzyme solution according to the present disclosure may also contain 0.2 to 0.5 wt.% chelating agent.

In the enzyme solution according to the present disclosure, the chelating agent can chelate metal ions that may be impurities in the enzyme solution, thereby increasing the cleaning ability of the enzyme solution.

Several chelating agents can be used as chelating agents in the enzyme solutions disclosed herein. According to some embodiments, the chelating agent includes at least one component, which is selected from the group consisting of ethylendiamine tetraacetic acid (EDTA) and diethylenetriamine pentaacetic acid.

According to certain embodiments, EDTA available from Sinopharm Chemical Reagent Co., Ltd., China can be selected as the ethylenediaminetetraacetic acid.

According to certain embodiments, Dissolvine D50 available from AKZO-Nobel Corporation can be selected as the diethylenetriaminepentaacetic acid.

Corrosion inhibitor

Based on the total weight of the enzyme solution, the enzyme solution according to the present disclosure may also contain 0.5 to 10 wt.% of an anticorrosive agent.

In the enzyme solution according to the present disclosure, the corrosion inhibitor helps delay or prevent the corrosion of the metal or rubber in contact with the enzyme solution.

Several corrosion inhibitors can be suitably used in the enzyme solution of the present disclosure. According to some embodiments, the corrosion inhibitor includes at least one component selected from the group consisting of borate, polyether, and higher alcohol.

According to certain embodiments, Crodacor BE-LQ-(AP) available from Croda Corporation can be selected as the borate.

Defoamer

Based on the total weight of the enzyme solution, the enzyme solution according to the present disclosure may also contain 0.1 to 0.3 wt.% defoamer.

In the enzyme solution according to the present disclosure, the antifoaming agent helps reduce or inhibit the foaming of the enzyme solution.

Several defoamers can be suitably used in the enzyme solution of the present disclosure. According to some embodiments, the antifoaming agent includes at least one component selected from the group consisting of silicone oil, polyether, and higher alcohol.

According to certain embodiments, DK1247 available from the Dow Corning Corporation can be selected as the defoamer.

Method of preparing enzyme solution

In some aspects, the present disclosure provides a method for preparing an enzyme solution, which includes the step of mixing the ingredients of the enzyme solution according to the present disclosure.

According to some embodiments, the method for preparing an enzyme solution includes the following steps: sequentially adding 6.9 to 10 wt.% of co-solvent, 0.1 to 10 wt.% of nonionic surfactant, and 0.75 to 6 wt.% of isothiazolinone , 0.1 to 1wt.% of benzisothiazolinone, 0.1 to 1wt.% of sodium benzoate, 0.03 to 15wt.% of room temperature enzyme stabilizer to 30 to 60wt.% of water to obtain the first solution; add 0.15 To 0.5wt.% of the pH adjuster to the first solution to obtain a second solution having a pH of 6 to 10; and adding 0.01 to 1.2wt.% of enzyme and 3 to 51wt.% of water to the first solution Two solutions to obtain the enzyme solution.

According to some embodiments, the method for preparing an enzyme solution includes the following steps: sequentially adding 6.9 to 10 wt.% of co-solvent, 0.1 to 10 wt.% of nonionic surfactant, and 0.75 to 6 wt.% of isothiazolinone , 0.1 to 1 wt.% benzisothiazolinone, 0.1 to 1 wt.% sodium benzoate, 0.03 to 15 wt.% room temperature enzyme stabilizer, 0.2 to 0.5 wt.% chelating agent, 0.5 to 10 wt.% Anti-corrosion agent, and 0.1 to 0.3wt.% defoamer to 30 to 60wt.% of water to obtain the first solution; add 0.15 to 0.5wt.% of pH adjuster to the first solution to obtain A second solution with a pH of 6 to 10; and adding 0.01 to 1.2 wt.% of enzyme and 3 to 51 wt.% of water to the second solution to obtain the enzyme solution.

For a detailed discussion of the components of the enzyme solution, please refer to the "Enzyme Solution" section of this patent specification.

Non-woven fabrics containing enzymes

In some aspects, the present disclosure provides an enzyme-containing non-woven fabric, which includes a non-woven fabric and an enzyme solution according to the present disclosure dispersed in the non-woven fabric, wherein the non-woven fabric contains 20 to 50 wt.% of viscose based on the total weight of the non-woven fabric Fiber and 50 to 80 wt.% polyester fiber; and the weight ratio of the enzyme solution and the non-woven fabric is 1.5:1 to 7:1.

For a detailed discussion of the enzyme solution, please refer to the "Enzyme Solution" section of this patent specification.

Several types of viscose fibers may be suitable for use in this disclosure. According to some embodiments, the viscose fiber can be prepared by the following method: alkalizing natural cellulose to obtain alkali Cellulose; reacting the alkalized cellulose with carbon disulfide to obtain cellulose xanthate; dissolving the cellulose xanthate in a diluted alkaline solution to obtain a viscous solution (viscose); and by The known wet spinning and post-processing procedures weave the viscous solution (viscose) into the viscose fiber.

Several types of polyester fibers may be suitable for use in this disclosure. According to some embodiments, the polyester fiber can be prepared by the following method: esterification or conversion of pure terephthalic acid (PTA) or dimethyl terephthalate (DMT) and ethylene glycol (EG) Esterification reaction and subsequent polycondensation reaction (polycondensation reaction) to obtain polyethylene phthalate (PET); and by known spinning and post-processing procedures to weave the polyethylene phthalate into poly Ester fiber.

When the amount of the viscose fiber or the polyester fiber is inconsistent with the above range, the enzyme in the enzyme solution can be absorbed by the non-woven fabric, reducing the compatibility of the enzyme solution with the non-woven fabric.

When the weight ratio of the enzyme solution to the non-woven fabric is less than 1.5:1, the cleansing ability of the enzyme solution in the enzyme-containing non-woven fabric may be reduced; and when the weight ratio of the enzyme solution to the non-woven fabric is greater than 7:1, it The maximum load of the enzyme solution in the non-woven fabric can be exceeded.

Method for preparing non-woven fabric containing enzyme

In some aspects, the present disclosure provides a method for preparing an enzyme-containing non-woven fabric, the method includes a step of allowing the enzyme solution according to the present disclosure to be dispersed into the non-woven fabric, and the weight ratio of the enzyme solution to the non-woven fabric is 1.5:1 to 7: 1.

For a detailed discussion of the enzyme solution, please refer to the "Enzyme Solution" section of this patent specification.

For a detailed discussion of the non-woven fabric, please refer to the "Enzyme-containing non-woven fabric" section of this patent specification.

Use of non-woven fabrics containing enzymes

In some aspects, the present disclosure provides the use of the enzyme-containing non-woven fabric according to the present disclosure to clean medical devices.

For the purpose of cleaning medical devices, the enzyme-containing non-woven fabric according to the present disclosure can be used to contact medical devices.

The following embodiments are intended to describe the present disclosure in an illustrative rather than restrictive manner.

Example 1 is an enzyme solution, based on the total weight of the enzyme solution, which contains 0.1 to 10wt.% of nonionic surfactant; 0.01 to 1.2wt.% of enzyme; 0.03 to 15wt.% of room temperature enzyme stabilizer; 0.75 to 6wt.% isothiazolinone (isothiazolinone); 0.1 to 1wt.% benzisothiazolinone; 0.1 to 1wt.% sodium benzoate; 6.9 to 10wt.% co-solvent; 0.15 to 0.5wt. % Of the pH adjuster; and 60 to 85wt.% of water; the enzyme solution has a pH of 6 to 10.

Embodiment 2 is the enzyme solution of embodiment 1, wherein the nonionic surfactant contains at least one component, which is selected from the group consisting of: (i) polymer, which is based on ethylene oxide and propylene oxide And at least one of butylene oxide; (ii) alkyl glucoside; (iii) fatty alcohol polyoxyethylene ether; and (iv) ammonium oxide.

Embodiment 3 is the enzyme solution of embodiment 1 or 2, wherein the enzyme contains at least one component selected from the group consisting of proteolytic enzymes, amylolytic enzymes, lipolytic enzymes, and cellulolytic enzymes.

Embodiment 4 is the enzyme solution of embodiment 3, wherein based on the total weight of the enzyme solution, the amount of the proteolytic enzyme is 0.05 to 0.3 wt.%, and the amount of the amylolytic enzyme is 0.01 to 0.1 wt.%, and The amount of the cellulolytic enzyme is 0.01 to 0.1 wt.%.

Embodiment 5 is the enzyme solution of any one of embodiments 1 to 4, wherein the isothiazolinone contains at least one component, which is selected from 5-chloro-2-methyl-4-isothiazolin-3 -Ketone and 2-methyl-4-isothiazolin-3-one.

Embodiment 6 is the enzyme solution of any one of Embodiments 1 to 5, wherein the benzisothiazolinone comprises 1,2-benzisothiazolin-3-one.

Embodiment 7 is the enzyme solution of any one of Embodiments 1 to 6, wherein the weight ratio of the isothiazolinone and the benzisothiazolinone is 3:1 to 30:1, and the isothiazolinone The weight ratio of the sodium benzoate is 1:1 to 30:1.

Embodiment 8 is the enzyme solution of any one of embodiments 1 to 7, wherein the room temperature enzyme stabilizer contains at least one component, which is selected from the group consisting of glycerin, propylene glycol, calcium chloride, and magnesium chloride , And boric acid.

Embodiment 9 is the enzyme solution of embodiment 8, wherein based on the total weight of the enzyme solution, the amount of the glycerin and/or propylene glycol is 5 to 10 wt.%, and the amount of calcium chloride and/or magnesium chloride is 0.03 to 0.7wt.%.

Embodiment 10 is the enzyme solution of any one of embodiments 1 to 9, wherein the cosolvent contains at least one component, which is selected from the group consisting of xylene sulfonic acid, sodium xylene sulfonate, Xylene sulfuric acid, sodium xylene sulfate, and alkylamine oxides.

Embodiment 11 is the enzyme solution of any one of embodiments 1 to 10, wherein the pH adjusting agent includes citric acid.

Embodiment 12 is the enzyme solution of any one of embodiments 1 to 11, wherein based on the total weight of the enzyme solution, the enzyme solution further contains 0.2 to 0.5 wt.% of a chelating agent.

Embodiment 13 is the enzyme solution of embodiment 12, wherein the chelating agent contains at least one component, which is selected from the group consisting of ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid.

Embodiment 14 is the enzyme solution of any one of embodiments 1 to 13, wherein based on the total weight of the enzyme solution, the enzyme solution further contains 0.5 to 10 wt.% of corrosion inhibitor.

Embodiment 15 is the enzyme solution of embodiment 14, wherein the corrosion inhibitor includes at least one component selected from the group consisting of borate, silicate, and polyaspartic acid.

Embodiment 16 is the enzyme solution of any one of embodiments 1 to 15, wherein based on the total weight of the enzyme solution, the enzyme solution further contains 0.1 to 0.3 wt.% of antifoaming agent.

Embodiment 17 is the enzyme solution of embodiment 16, wherein the defoaming agent contains at least one component selected from the group consisting of silicone oil, polyether, and higher alcohol.

Embodiment 18 is the enzyme solution of any one of embodiments 1 to 17, wherein the enzyme solution has a pH value of 7-9.

Example 19 is a method of preparing an enzyme solution, which includes a step of mixing the ingredients of the enzyme solution of any one of Examples 1 to 18.

Embodiment 20 is the method of embodiment 19, which includes the following steps: sequentially adding 6.9 to 10 wt.% of cosolvent, 0.1 to 10 wt.% of nonionic surfactant, and 0.75 to 6 wt.% of isothiazolinone , 0.1 to 1wt.% of benzisothiazolinone, 0.1 to 1wt.% of sodium benzoate, 0.03 to 15wt.% of room temperature enzyme stabilizer to 30 to 60wt.% of water to obtain the first solution; add 0.15 To 0.5wt.% of the pH adjuster to the first solution to obtain a second solution having a pH of 6 to 10; and adding 0.01 to 1.2wt.% of enzyme and 3 to 51wt.% of water to the first solution Two solutions to obtain the enzyme solution.

Example 21 is the method of Example 19, which includes the following steps: sequential addition of 6.9 to 10 wt.% of co-solvent, 0.1 to 10 wt.% of nonionic surfactant, and 0.75 to 6 wt.% of isothiazolinone , 0.1 to 1 wt.% benzisothiazolinone, 0.1 to 1 wt.% sodium benzoate, 0.03 to 15 wt.% room temperature enzyme stabilizer, 0.2 to 0.5 wt.% chelating agent, 0.5 to 10 wt.% Anti-corrosion agent, and 0.1 to 0.3wt.% defoamer to 30 to 60wt.% of water to obtain the first solution; add 0.15 to 0.5wt.% of pH adjuster to the first solution to obtain PH 6 to 10 的 second solution; and adding 0.01 to 1.2 wt.% of enzyme and 3 to 51 wt.% of water to the second solution to obtain the enzyme solution.

Embodiment 22 is an enzyme-containing non-woven fabric, which comprises a non-woven fabric and the enzyme solution of any one of embodiments 1 to 18 dispersed in the non-woven fabric, wherein the non-woven fabric contains 20 to 50 wt.% based on the total weight of the non-woven fabric Viscose fiber and 50 to 80 wt.% polyester fiber; and the weight ratio of the enzyme solution and the non-woven fabric is 1.5:1 to 7:1.

Example 23 is a method for preparing an enzyme-containing non-woven fabric, which includes a step of allowing the enzyme solution as in any one of Examples 1 to 18 to be dispersed into a non-woven fabric, wherein the non-woven fabric contains 20 to 50 wt based on the total weight of the non-woven fabric. % Of viscose fiber and 50 to 80wt.% of polyester fiber; and the weight ratio of the enzyme solution and the non-woven fabric is 1.5:1 to 7:1.

Example 24 is the use of the enzyme-containing non-woven fabric of Example 22 in a clean medical device.

Instance

The examples and comparative examples provided below are intended to help understand the present invention, but should not be regarded as limiting the scope of the present invention. All parts and percentages are by weight unless otherwise specified.

The raw materials used in the examples of the present invention and the comparative examples are shown in Table 1 below.

Preparation of enzyme solution

Under the conditions of normal temperature (10 to 25°C) and normal pressure (approximately 1 atmosphere pressure), add the ingredients of the enzyme solution to a glass or stainless steel container based on the total weight of the enzyme solution, and mix the ingredients according to the following steps Obtain the enzyme solution: add 6.9 to 10wt.% of cosolvent, 0.1 to 10wt.% of non-ionic surfactant, 0.75 to 6wt.% of isothiazolinone, and 0.1 to 1wt.% of benzisothiazole. Linone, 0.1 to 1wt.% of sodium benzoate, 0.03 to 15wt.% of room temperature enzyme stabilizer to 30 to 60wt.% of water to obtain the first solution; add 0.15 to 0.5wt.% of pH adjuster to the The first solution to obtain a second solution having a pH value of 6 to 10; and 0.01 to 1.2 wt.% of enzyme and 3 to 51 wt.% of water are added to the second solution to obtain the enzyme solution.

In addition, the first solution may further include 0.2 to 0.5 wt.% chelating agent, 0.5 to 10 wt.% corrosion inhibitor, and 0.1 to 0.3 wt.% defoaming agent based on the total weight of the enzyme solution as needed.

Preparation of non-woven fabrics containing enzymes

Under the conditions of normal temperature (10 to 25°C) and normal pressure (about 1 atmospheric pressure), disperse the enzyme solution to the non-woven fabric in a weight ratio of 1.5:1 to 7:1 to obtain the enzyme-containing The non-woven fabric.

According to some embodiments, the enzyme solution can be dispersed into the non-woven fabric by suitable means such as spraying and soaking to obtain the enzyme-containing non-woven fabric.

In this disclosure, the cleansing ability of the enzyme solution is evaluated according to the "Enzyme solution cleansing ability test".

In this disclosure, the enzyme activity in the enzyme-containing non-woven fabric is evaluated according to the "enzyme activity test".

In this disclosure, the compatibility of the non-woven fabric and the enzyme solution in the enzyme-containing non-woven fabric is evaluated in accordance with the "Enzyme Solution and Non-woven Fabric Compatibility Test".

In this disclosure, the enzyme stability in the non-woven fabric containing enzymes is evaluated in accordance with the "Enzyme Stability Test".

In this disclosure, the anti-fungal and anti-bacterial ability of the enzyme-containing non-woven fabric is evaluated according to the "microbial challenge test".

In this disclosure, the degree of damage of the enzyme-containing non-woven fabric to the medical device is assessed according to the "endoscope damage test".

In this disclosure, the corrosiveness of the enzyme solution in the enzyme-containing non-woven fabric is evaluated according to the "corrosion test".

Cleansing ability test of enzyme solution

One of the X-OMAT BT medical X-ray films available from Kodak Corporation (the X-OMAT BT medical X-ray film has the model of XBT-1 and has gelatin on its surface) is woven into a test piece, the test piece It has a length of 8.0±1.0mm and a width of 3.0±1.0mm.

Adjust the constant temperature water bath to the specified temperature (37 to 40°C) and maintain the temperature.

Use a 100ml beaker to take 80ml of the enzyme solution to be tested.

Place the beaker containing the enzyme solution to be tested in a constant temperature water bath and incubate until the system reaches a stable temperature.

Place the test piece close to the mouth of the beaker and put it into the constant temperature enzyme solution to be tested, and start timing.

Was visually observed and recorded on the gelatin coating of the test piece begins to dissolve the gelatin coating times t ₁ and a time to completely dissolve t _2.

Calculate the flushing time t, t=t ₂ -t ₁ .

If the washing time t is less than 8 minutes, it shows that the washing ability of the tested enzyme solution is acceptable.

Enzyme activity test

The test was performed using the Gallery instrument available from Thermo Fisher Corporation and according to the standard test method "Proteolytic enzyme activity test in mixed samples" provided by Novozymes Corporation.

Compatibility test of enzyme solution and non-woven fabric

Test the enzyme activity in the newly prepared enzyme solution and record the activity as a ₁ .

Mix the enzyme solution and the non-woven fabric in a weight ratio of 1.5:1 to 7:1 to obtain an enzyme-containing non-woven fabric, let the fabric stand for two weeks, then squeeze the enzyme solution from the cloth and test the enzyme in the extruded enzyme solution Activity, and record the activity as a ₂ .

Suppose X ₁ = (a1-a2)/a1×100%.

If X ₁ <5%, it indicates that the enzyme solution in the enzyme-containing non-woven fabric has good compatibility with the non-woven fabric.

Enzyme stability test

Enzyme stability test includes "Enzyme stability test under normal temperature (10-25℃) conditions" and "Enzyme stability test under relatively high temperature (above 25℃) conditions".

Enzyme stability test under normal temperature (10 to 25℃)

Test the enzyme activity in the newly prepared enzyme solution and record the activity as a ₁ .

Mix the enzyme solution with the non-woven fabric in a weight ratio of 1.5:1 to 7:1 to obtain a non-woven fabric containing enzymes.

Let the cloth stand for 3 months under the conditions of "25°C and 30 to 80% humidity".

The enzyme solution was extruded from the cloth and the enzyme activity in the extruded enzyme solution was tested, and the activity was recorded as a ₃ .

Assume that X ₂ =(a1-a3)/a1×100%.

If X ₂ <15%, it shows that the enzyme solution in the enzyme-containing non-woven fabric has good enzyme stability at room temperature (20-25°C).

Enzyme stability test under relatively high temperature (above 25°C): Test the enzyme activity in the newly prepared enzyme solution and record the activity as a ₁ .

Mix the enzyme solution with the non-woven fabric in a weight ratio of 1.5:1 to 7:1 to obtain a non-woven fabric containing enzymes.

Let the cloth stand for 2 weeks under the conditions of "54°C and 75% humidity".

The enzyme solution was extruded from the cloth and the enzyme activity in the extruded enzyme solution was tested, and the activity was recorded as a ₄ .

Assume X ₃ = (a1-a4)/a1×100%.

If X ₂ <25%, it indicates that the enzyme solution in the enzyme-containing non-woven fabric has good enzyme stability under relatively high temperature (above 25°C) conditions.

Microbiological challenge test

Perform the microbiological challenge test according to the method of GB-15979-2002: Use an electronic scale to weigh 50 grams of enzyme-containing non-woven fabric.

Choose mold suspension ( Aspergillus niger , Penicillium citrinum , Gliocladium virens , Chaetomium globosum and Aureobaidsium pullulans ) or bacterial suspension ( E.coli , Staphylococcus aureus , Bacillus megaterium , Pseudomonas fluorescens and Bacillus ).

To 1ml of the test bacterial suspension was injected into the mold or the enzyme solution and stirred with a sterile glass rod was homogeneous, to obtain samples microorganism solution having a concentration of about mold ¹⁰⁵ to 10 ⁶ / ml in or bacteria.

Let the above-mentioned microbial solution sample stand in a constant temperature incubator at a specific temperature for 28 days (the cultivation temperature of mold is 28±1°C, and the cultivation temperature of bacteria is 36±1°C).

Every seven days, perform a count or plate streaking of the survival of molds or bacteria. If there is no colony growth, it indicates that the enzyme solution has passed the challenge test against mold or bacteria, and if there is colony growth, it indicates that the enzyme solution has failed the challenge test against mold or bacteria, and there is a risk of mold or bacteria growing in it.

Endoscope damage test

Use enzyme-containing non-woven fabric to rub the outer surface of the endoscope 100 times at "100N, 10cm/s". If the outer surface of the endoscope has no obvious scratches after being rubbed by visual inspection, it shows that the non-woven fabric containing enzymes is less likely to cause damage to the medical device.

Corrosion test

According to the requirements of the "Technical Standards for Disinfection-2002" formulated by the Ministry of Health of China, the corrosion test is performed: grinding a piece of metal sheet (stainless steel sheet) or rubber sheet (styrene butadiene) Diene rubber), wash the sheet and weigh the sheet, and record the weight as a ₅ .

Put the metal sheet or the rubber sheet into 200ml of the enzyme solution to be tested, soak the sheet for 72 hours, and then remove the sheet.

First rinse the metal sheet or rubber sheet with water, then clean the surface of the sheet with a brush or other soft tools (if any corrosion products are present on the surface of the sheet, they must be removed from the sheet), and then weighed For heavy sheets, record the weight as a ₄ .

Suppose X ₄ = (a5-a4).

If X ₄ =0, it shows that the enzyme solution does not corrode the metal sheet or the rubber sheet, and therefore passes the corrosion test for the metal sheet or the rubber sheet.

If X ₄ >0, it shows that the enzyme solution corrodes the metal sheet or the rubber sheet, and therefore does not pass the corrosion test for the metal sheet or the rubber sheet.

Examples 1 to 13 and Comparative Examples 1 to 7

The enzyme solution and the enzyme-containing non-woven fabric are prepared by the above method according to the ingredients and amounts listed in Table 2a, Table 2b, and Table 2c (the amounts listed in Table 2a, Table 2b, and Table 2c are all Calculated by weight percentage).

"Enzyme solution cleansing ability test", "Enzyme solution and non-woven compatibility test", "Enzyme stability test", "Microbiological challenge test", "Endoscope damage "Hazard Test" and "Corrosion Test" are performed according to the above methods respectively. The results are shown in Table 3, Table 4, Table 5, Table 6, Table 7, and Table 8, respectively.

According to Table 2a, Table 2b, Table 2c, and Table 3, the enzyme solutions in the enzyme-containing non-woven fabrics provided in Examples 1 to 13 have good cleaning ability.

According to Table 2a, Table 2b, Table 2c, and Table 4, the following may be known.

Among the enzyme-containing non-woven fabrics provided in Examples 1 to 13, the non-woven fabric contains an appropriate amount of viscose fiber and polyester fiber, and the enzyme solution contains 0.75 to 6 wt.% isothiazolinone and 0.1 to 1 wt.% benzo Isothiazolinone and 0.1 to 1 wt.% sodium benzoate, so the non-woven fabric has good compatibility with enzymes.

Among the enzyme-containing non-woven fabrics provided in Comparative Example 1, the non-woven fabric only contains bamboo fibers, and the enzyme solution contains neither benzisothiazolinone nor sodium benzoate, so the non-woven fabric is relatively incompatible with enzymes.

Among the enzyme-containing non-woven fabrics provided in Comparative Example 2, the non-woven fabric only contains viscose fibers, so the non-woven fabric is relatively incompatible with enzymes.

In the enzyme-containing non-woven fabric provided in Comparative Example 3, although the non-woven fabric contains an appropriate amount of viscose fiber and polyester fiber, the enzyme solution does not contain an appropriate amount of isothiazolinone, benzisothiazolinone, and sodium benzoate. Therefore, non-woven fabrics are less compatible with enzymes.

According to Table 2a, Table 2b, Table 2c, and Table 4, the following may be known.

In the enzyme-containing non-woven fabrics provided in Examples 1 to 13, the enzyme solution contains 0.03 to 15 wt.% of room temperature enzyme stabilizer, so the enzyme solution dispersed in the non-woven fabric has good performance at room temperature (10 to 25°C). Enzyme stability.

In the enzyme-containing non-woven fabrics provided in Examples 1 to 13, the enzyme solution contains 0.75 to 6 wt.% isothiazolinone, 0.1 to 1 wt.% benzisothiazolinone, and 0.1 to 1 wt.% benzene Sodium formate, so the enzyme solution dispersed in the non-woven fabric has good enzyme stability under relatively high temperature (above 25°C).

In the enzyme-containing non-woven fabric provided in Comparative Example 1, the enzyme solution contains 0.3wt.% isothiazolinone, without benzisothiazolinone or sodium benzoate, Therefore, the enzyme solution dispersed in the non-woven fabric does not have sufficient enzyme stability under relatively high temperature (above 25°C) conditions.

In the enzyme-containing non-woven fabric provided in Comparative Example 4, the enzyme solution contains 0.5wt.% isothiazolinone, 0.1wt.% benzisothiazolinone, and 0.5wt.% sodium benzoate, isothiazole The amount of linone is insufficient, so the enzyme solution dispersed in the non-woven fabric does not have sufficient enzyme stability under relatively high temperature (above 25°C) conditions.

In the enzyme-containing non-woven fabric provided in Comparative Example 5, the enzyme solution contained 0.5wt.% isothiazolinone, 1wt.% benzisothiazolinone, and 0.5wt.% sodium benzoate, isothiazoline The amount of ketone is insufficient, so the enzyme solution dispersed in the non-woven fabric does not have sufficient enzyme stability under relatively high temperature (above 25°C) conditions.

In the enzyme-containing non-woven fabric provided in Comparative Example 6, the enzyme solution contained 1.5 wt.% isothiazolinone and 0.1 wt.% benzisothiazolinone, and did not contain sodium benzoate, so the enzyme solution was dispersed in the non-woven fabric The enzyme solution does not have sufficient enzyme stability under relatively high temperature (above 25°C) conditions.

In the enzyme-containing non-woven fabric provided in Comparative Example 7, the enzyme solution contained 1.5wt.% of isothiazolinone and 0.5wt.% of sodium benzoate, and did not contain benzisothiazolinone, so it was dispersed in the non-woven fabric. The enzyme solution does not have sufficient enzyme stability under relatively high temperature (above 25°C) conditions.

According to Table 2a, Table 2b, Table 2c, and Table 6, the following may be known.

In the enzyme-containing non-woven fabrics provided in Examples 1 to 13, the enzyme solution contains 0.75 to 6 wt.% isothiazolinone, 0.1 to 1 wt.% benzisothiazolinone, and 0.1 to 1 wt.% benzene Sodium formate, so the enzyme solution dispersed in the non-woven fabric has good anti-fungal and anti-bacterial properties.

In the non-woven fabric containing enzymes provided in Comparative Example 3, the enzyme solution contains 0.3wt.% of isothiazolinone and does not contain benzisothiazolinone or sodium benzoate. Therefore, the enzyme solution dispersed in the non-woven fabric does not have enough The anti-fungal and anti-bacterial properties.

In the enzyme-containing non-woven fabric provided in Comparative Example 4, the enzyme solution contains 0.5wt.% isothiazolinone, 0.1wt.% benzisothiazolinone, and 0.5wt.% sodium benzoate, isothiazole The amount of linone is insufficient, so the enzyme solution dispersed in the non-woven fabric does not have sufficient anti-fungal and anti-bacterial properties.

In the enzyme-containing non-woven fabric provided in Comparative Example 5, the enzyme solution contained 0.5wt.% isothiazolinone, 1wt.% benzisothiazolinone, and 0.5wt.% sodium benzoate, isothiazoline The amount of ketone is insufficient, so the enzyme solution dispersed in the non-woven fabric does not have sufficient anti-fungal and anti-bacterial properties.

In the enzyme-containing non-woven fabric provided in Comparative Example 6, the enzyme solution contained 1.5 wt.% isothiazolinone and 0.1 wt.% benzisothiazolinone, and did not contain sodium benzoate, so the enzyme solution was dispersed in the non-woven fabric The enzyme solution does not have sufficient anti-fungal and anti-bacterial properties.

In the enzyme-containing non-woven fabric provided in Comparative Example 7, the enzyme solution contained 1.5wt.% of isothiazolinone and 0.5wt.% of sodium benzoate, and did not contain benzisothiazolinone, so it was dispersed in the non-woven fabric. The enzyme solution does not have sufficient anti-fungal and anti-bacterial properties.

According to Table 2a, Table 2b, and Table 7, the enzyme-containing non-woven fabrics provided in Examples 1 to 9 are less likely to cause damage to medical devices.

According to Table 2a, Table 2b, Table 2c, and Table 8, the enzyme-containing non-woven fabrics provided in Examples 1 to 13 are less likely to cause corrosion to the metal or rubber components of medical devices.

In summary, the enzyme solution according to the present disclosure has good cleaning ability. In addition, the enzyme solution according to the present disclosure contains an appropriate amount of isothiazolinone, benzisothiazolinone, and sodium benzoate, so the enzyme solution has good compatibility with non-woven fabrics containing viscose fibers and polyester fibers. The enzyme solution dispersed in the non-woven fabric not only has good enzyme stability, but also has good anti-fungal and anti-bacterial properties. In addition, According to the present disclosure, the enzyme-containing non-woven fabric is less likely to cause damage to the medical device, and less likely to cause corrosion of the metal or rubber components of the medical device.

Although the detailed description mentioned above contains many specific details for illustrative purposes, those with ordinary knowledge in the technical field will understand that many changes, alterations, substitutions, and alterations to the details are as claimed in the scope of the patent application. The scope of the invention. Therefore, the present disclosure described in the detailed description is presented without imposing any restrictions on the claimed invention. The proper scope of the present invention should be determined by the scope of the following patent applications and the appropriate legal equivalents. The full text of all references cited is incorporated herein by reference.

Claims (21)

An enzyme solution, based on the total weight of the enzyme solution, which contains: 0.1 to 10wt.% of nonionic surfactant; 0.01 to 1.2wt.% of enzyme; 0.03 to 15wt.% of room temperature enzyme stabilizer; 0.75 to 6wt.% isothiazolinone; 0.1 to 1wt.% benzoisothiazolinone; 0.1 to 1wt.% sodium benzoate; 6.9 to 10wt.% co-solvent; 0.15 to 0.5wt.% % Of pH adjuster; and 60 to 85wt.% of water; the enzyme solution has a pH of 6 to 10. The enzyme solution of claim 1, wherein the nonionic surfactant comprises at least one component, which is selected from the group consisting of: (i) polymers based on ethylene oxide and propylene oxide (propylene oxide) or at least one of butylene oxide; (ii) alkyl glucoside; (iii) fatty alcohol polyoxyethylene ether; and (iv) ) Ammonium oxide. The enzyme solution of claim 1, wherein the enzyme comprises at least one component selected from the group consisting of proteolytic enzymes, amylolytic enzymes, lipolytic enzymes, and cellulolytic enzymes. The enzyme solution of claim 1, wherein the room temperature enzyme stabilizer contains at least one component, which is selected from the group consisting of glycerin, propylene glycol, calcium chloride, magnesium chloride, and boric acid. The enzyme solution of claim 1, wherein the isothiazolinone contains at least one component, which is It is selected from the group consisting of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one. The enzyme solution of claim 1, wherein the benzisothiazolinone comprises 1,2-benzisothiazolin-3-one. The enzyme solution of claim 1, wherein the weight ratio of the isothiazolinone and the benzisothiazolinone is 3:1 to 30:1, and the weight ratio of the isothiazolinone and the sodium benzoate is 1: 1 to 30:1. The enzyme solution of claim 1, wherein the co-solvent contains at least one component, which is selected from the group consisting of xylene sulfonic acid, sodium xylene sulfonate, xylene sulfuric acid, sodium xylene sulfate, and Alkylamine oxide. The enzyme solution of claim 1, wherein the pH adjusting agent contains citric acid. According to the enzyme solution of claim 1, wherein based on the total weight of the enzyme solution, the enzyme solution further contains 0.2 to 0.5 wt.% of a chelating agent. The enzyme solution of claim 10, wherein the chelating agent comprises at least one component, which is selected from the group consisting of ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid. According to the enzyme solution of claim 1, wherein based on the total weight of the enzyme solution, the enzyme solution further contains 0.5 to 10 wt.% of corrosion inhibitor. The enzyme solution of claim 12, wherein the corrosion inhibitor comprises at least one component selected from the group consisting of borate, polyether, higher alcohol, silicate, and polyaspartic acid group. According to the enzyme solution of claim 1, wherein based on the total weight of the enzyme solution, the enzyme solution further contains 0.1 to 0.3 wt.% of antifoaming agent. The enzyme solution of claim 14, wherein the defoaming agent comprises at least one component selected from the group consisting of silicone oil, polyether, and higher alcohol. A method of preparing an enzyme solution, which comprises a step of mixing the ingredients of the enzyme solution according to any one of claims 1 to 15. The method of claim 16, wherein based on the total weight of the enzyme solution, the method comprises the following steps: sequentially adding 6.9 to 10wt.% of co-solvent, 0.1 to 10wt.% of nonionic surfactant, 0.75 to 6wt. % Isothiazolinone, 0.1 to 1 wt.% benzisothiazolinone, 0.1 to 1 wt.% sodium benzoate, 0.03 to 15 wt.% room temperature enzyme stabilizer to 30 to 60 wt.% water to obtain The first solution; add 0.15 to 0.5wt.% of the pH adjuster to the first solution to obtain a second solution having a pH of 6 to 10; and add 0.01 to 1.2wt.% of enzymes and 3 to 51wt. % Water into the second solution to obtain the enzyme solution. The method of claim 16, wherein based on the total weight of the enzyme solution, the method comprises the following steps: sequentially adding 6.9 to 10wt.% of co-solvent, 0.1 to 10wt.% of nonionic surfactant, 0.75 to 6wt. % Isothiazolinone, 0.1 to 1 wt.% benzisothiazolinone, 0.1 to 1 wt.% sodium benzoate, 0.03 to 15 wt.% room temperature enzyme stabilizer, 0.2 to 0.5 wt.% chelating agent, 0.5 to 10wt.% of anti-corrosion agent and 0.1 to 0.3wt.% of defoamer to 30 to 60wt.% of water to obtain the first solution; add 0.15 to 0.5wt.% of pH adjuster to the first Solution to obtain a second solution having a pH value of 6 to 10; and adding 0.01 to 1.2 wt.% of enzyme and 3 to 51 wt.% of water to the second solution to obtain the enzyme solution. An enzyme-containing non-woven fabric, which comprises a non-woven fabric and the enzyme solution according to any one of claims 1 to 15 dispersed in the non-woven fabric; wherein, based on the total weight of the enzyme solution, the non-woven fabric contains 20 to 50 wt.% of adhesive Glue fiber and 50 to 80 wt.% polyester fiber (terylene fiber); and the weight ratio of the enzyme solution and the non-woven fabric is 1.5:1 to 7:1. A method of preparing an enzyme-containing non-woven fabric, which comprises a step of allowing the enzyme solution as claimed in any one of claims 1 to 15 to be dispersed into a non-woven fabric, wherein the non-woven fabric contains 20 to 50 wt.% of adhesive based on the total weight of the non-woven fabric Glue fiber and 50 to 80 wt.% polyester fiber; and the weight ratio of the enzyme solution and the non-woven fabric is 1.5:1 to 7:1. A use of the enzyme-containing non-woven fabric in a clean medical device as in claim 19.