TWI677345B - Use of extract of artocarpus heterophyllus white core for regulating expression of cfos gene, csf1r gene, rank gene, syk gene, trap gene, apa1 gene, abca1 gene, il-6 gene, vcam1 gene, casp8 gene, ddc gene and asmtl protein gene, and improving menopausal - Google Patents

Abstract

The invention provides an extract of jackfruit white core for regulating CFOS gene, CSF1R gene, RANK gene, SYK gene, TRAP gene, APA1 gene, ABCA1 gene, IL-6 gene, VCAM1 gene, CASP8 gene, DDC gene, and ASMTL. Expression of protein genes, and uses for improving menopausal syndrome.

Description

The extract of jackfruit white core is used to regulate the CFOS gene, CSF1R gene, RANK gene, SYK gene, TRAP gene, APA1 gene, ABCA1 gene, IL-6 gene, VCAM1 gene, CASP8 gene, DDC gene, and ASMTL protein gene expression, and uses for improving menopause syndrome

The invention relates to an extract of jackfruit ( Artocarpus heterophyllus ) white core for regulating CFOS gene, CSF1R gene, RANK gene, SYK gene, TRAP gene, APA1 gene, ABCA1 gene, IL-6 gene, VCAM1 gene, CASP8 gene , DDC gene, and ASMTL protein gene expression, and the use of improving menopause syndrome.

Menopause is associated with the rapid decrease of circulating sexual hormones in women, and means that women's ovarian function gradually declines, eventually leading to a transition period of cessation of menstruation, and Postmenopause refers to the period after a woman's ovarian function is completely lost. Menopausal syndrome usually occurs in menopausal or postmenopausal women, and the symptoms of menopausal syndrome include hot flushes, osteoporosis, cardiovascular disease, and insomnia. ), Skin aging, anxiety, and depression.

At present, in the treatment of menopausal syndrome, hormone replacement therapy (HRT) is mostly used, and medicines containing one or more sex hormones are administered to women during or after menopause to supplement their insufficient sex hormones. However, long-term administration or excessive use of sex hormones may cause breast cancer and have side effects. Therefore, if we can develop medicines or food products that naturally have no side effects and can improve menopausal syndromes, it will benefit the broader ethnic groups in need and bring about a considerable breakthrough in the technology in this field.

In view of this, an object of the present invention is to provide an extract of white core of Artocarpus heterophyllus for preparing a CFOS gene, a colony stimulating factor 1 receptor (CSF1R) gene, a RANK gene, and a spleen. Spleen tyrosinase kinase (SYK) gene, tartrate resistant acid phosphatase (TRAP) gene, apolipoprotein A-1 (APA1) gene, ATP-binding cassette family A member 1 (ATP binding cassette subfamily A member 1, ABCA1) gene, interleukin-6 (IL-6) gene, vascular cell adhesion molecule 1, VCAM1 gene, apoptotic protease 8 ( caspase 8, CASP8) gene, DOPA decarboxylase (DDC) gene, and Acetylserotonin O-methyltransferase-like protein (ASMTL protein) gene Or food products.

Another object of the present invention is to provide an extract of jackfruit white core for preparing a medicinal or food product for improving menopause symptoms.

In an embodiment of the present invention, the jackfruit white core extract is prepared by extracting the jackfruit white core with water or alcohol as an extraction solvent.

In an embodiment of the present invention, the liquid-solid ratio of the extraction solvent to the jackfruit white core is between 2 and 10: 1 and 5.

In one embodiment of the present invention, the extraction temperature is between 50 ° C and 100 ° C.

In one embodiment of the present invention, the extraction time is between 0.5 and 3 hours.

In one embodiment of the invention, the pharmaceutical product comprises a pharmaceutically acceptable carrier.

In an embodiment of the invention, the menopausal syndrome includes osteoporosis, cardiovascular disease, hot flushes, and insomnia.

In summary, the efficacy of the jackfruit white core extract of the present invention is that it can regulate the CFOS gene, CSF1R gene, RANK gene, SYK gene, TRAP gene, APA1 gene, ABCA1 gene, IL-6 gene, VCAM1 gene, CASP8 gene , DDC genes, and ASMTL protein genes, and by inhibiting osteoclast differentiation and bone resorption, promoting high density lipoprotein (HDL) production and cholesterol metabolism, reducing endothelial cell inflammation and Apoptosis, and increase the production of serotonin and melatonin, to achieve the effect of improving menopause syndromes (including osteoporosis, cardiovascular disease, hot flushes, and insomnia).

The embodiments of the present invention will be further described below. The examples listed below are intended to clarify the present invention, and are not intended to limit the scope of the present invention. As some changes and retouching can be done, the scope of protection of the present invention shall be determined by the scope of the attached patent application.

FIG. 1 is a cell staining diagram of the effectiveness of the jackfruit white core extract in inhibiting osteoclast differentiation.

FIG. 2 is a data chart of the efficacy of the jackfruit white core extract of the present invention in regulating the expression of genes related to osteoclast differentiation (including CFOS gene, CSF1R gene and RANK gene), where “***” indicates that 2 comparison, p <0.001.

FIG. 3 is a data chart of the efficacy of the jackfruit white core extract of the present invention in regulating the expression of genes (including SYK gene and TRAP gene) related to bone resorption of osteoclasts, where “***” indicates comparison with control group 2 , P <0.001.

FIG. 4 is a data chart of the efficacy of the extract of jackfruit white core according to the present invention in regulating the expression of genes (including APA1 gene and ABCA1 gene) related to HDL production and cholesterol metabolism, where “*” indicates that compared with the control group, p <0.05;"***" indicates p <0.001 compared with the control group.

FIG. 5 is a data chart showing the efficacy of the jackfruit white core extract of the present invention in regulating the expression of genes associated with endothelial cell inflammation and apoptosis (including IL-6 gene, VCAM1 gene and CASP8 gene), where “*” Compared with the control group, p <0.05;"***" means compared with the control group, p <0.001.

Fig. 6 is a data chart of the efficacy of the extract of jackfruit white core of the present invention in regulating the expression of genes related to tryptophan metabolism (including DDC gene and ASMTL gene), where "***" indicates comparison with the control group, p <0.001.

definition

The values used in this article are approximate. All experimental data are shown in the range of 20%, preferably in the range of 10%, and most preferably in the range of 5%.

As used herein, the term "menopause" means a period between the beginning of the decline of ovarian function and the cessation of menstruation.

As used herein, the term "menopausal syndrome" means symptoms that occur in female individuals during or after menopause, and include, but are not limited to: (1) physiological symptom, such as Hot flushes, vaginal dryness, fatigue, headaches, cardiovascular disease, insomnia, weight gain, skin aging And osteoporosis; (2) psychological symptoms, such as anxiety and depression; and (3) cognitive deficits, such as mental confusion, memory (memory ability), learning ability (recognition ability), and inability to cope.

According to one embodiment of the present invention, the jackfruit ( Artocarpus heterophyllus ) is aliased as jackfruit, wood jack, tree jack or melon, and is an evergreen tree of the genus Morraceae and Artocarpus . Jackfruit's pulp is a fruit with a sweet smell and is often used as a cooking material; seeds are also edible, but raw food may cause poisoning.

According to the present invention, pharmaceuticals can be manufactured into a dosage form suitable for parenterally or orally administration using techniques well known to those skilled in the art. This includes, but is not Limited to injections [eg, sterile aqueous solution or dispersion], sterile powders, tablets, troche, pills ), Capsules and the like.

According to the present invention, pharmaceuticals can be administered parenterally from a group selected from the group consisting of intraperitoneal injection, subcutaneous injection, intramuscular injection, Intravenous injection, sublingual administration, and transdermal administration.

According to the present invention, the pharmaceutical product may further include a pharmaceutically acceptable carrier that is widely used in pharmaceutical manufacturing technology. For example, the pharmaceutically acceptable carrier may include one or more agents selected from the group consisting of a solvent, a buffer, an emulsifier, a suspending agent, and a decomposer. ), Disintegrating agent, dispersing agent, binding agent, excipient, stabilizing agent, chelating agent, diluent , Gelling agent, preservative, wetting agent, lubricant, absorption delaying agent, liposome, and the like. The selection and quantity of these reagents fall within the professionalism and routine skills of those familiar with the technology.

According to the present invention, the pharmaceutically acceptable carrier includes a solvent selected from the group consisting of water, normal saline, phosphate buffered saline (PBS), Aqueous solution containing alcohol and combinations thereof.

According to the present invention, the composition can be regarded as a food additive, which can be added by conventional methods during the preparation of raw materials, or added during the process of food production, and formulated with any kind of edible material. Food products for human and non-human animals.

According to the present invention, the types of food products include, but are not limited to, beverages, fermented foods, bakery products, health foods, and dietary supplements.

Example 1. Preparation of extract of jackfruit white core

First, prepare the white core of jackfruit and clean it for subsequent extraction. Next, take the washed jackfruit white core and homogenize it, and then extract the homogenate with water (2 ~ 10: 1 ~ 5 liquid-solid ratio) at 50 ~ 100 ℃ for 0.5 ~ 3 hours. After that, it was cooled to room temperature and filtered through a 400 mesh filter, and then the filtered product was concentrated under reduced pressure at 45 to 70 ° C. to obtain an extract of jackfruit white core.

Example 2. Evaluation of the effectiveness of jackfruit white core extract in improving osteoporosis 2.1 Inhibition of osteoclast differentiation

First, peripheral blood (PB) was provided by a healthy donor, and then 20 mL of peripheral blood was diluted with 15 mL of PBS. Next, 15 mL of Ficoll (GE Healthcare) was added to a centrifuge tube having a volume of 50 mL, and then 35 mL of diluted peripheral blood was added to the polysucrose layer, and centrifuged at 400 g for 40 minutes. After that, peripheral blood mononuclear cells (PBMCs) were removed and washed twice with PBS.

Then, peripheral blood mononuclear cells were divided into two groups, including a control group and an experimental group. 10% fetal bovine serum (FBS) (Gibco), 1X penicillin / streptomycin (Gibco), 40ng / mL human nuclear factor κ-B ligand receptor activator (receptor activator) of nuclear factor kappa-B ligand (RANKL) (Peprotech, 310-01) and 25ng / mL human monocyte colony stimulating factor (M-CSF) (Peprotech, 300-25) α-MEM ( (Gibco, 12000-022) was used as osteoclast differentiation medium to culture cells of each group for 14 days, in which cells in the experimental group were replaced every 3 days with osteoblast differentiation medium supplemented with 0.25 mg / mL jackfruit white core extract The cells in the control group were replaced with osteoclast differentiation medium every 3 days.

After 14 days of culture, a large number of multinucleated osteoclasts were produced, followed by 1 drop of Actin Green ^TM 488 ReadyProbes ^® reagent (Thermo, R37110) and Hurst 33342 (Hoechst 33342) (Thermo, 62249) (1: 20,000-fold dilution) The cells were stained for 15 minutes, and then the osteoclasts were observed with a fluorescent microscope. The results of this experiment are shown in Figure 1.

FIG. 1 is a cell staining diagram of the effectiveness of the jackfruit white core extract in inhibiting osteoclast differentiation. As can be seen from FIG. 1, compared with the control group, the cells in the experimental group were mostly undifferentiated monocytes, while the cells in the control group had a multi-nucleus structure with the characteristics of osteoclasts. The result of this experiment It is shown that the extract of jackfruit white core of the present invention can achieve the effect of improving osteoporosis by inhibiting osteoclast differentiation.

2.2 Analysis of gene expression related to osteoclast differentiation and bone resorption

First, the peripheral blood mononuclear cells according to Example 2.1 were divided into three groups, including two control groups (that is, control group 1 and control group 2) and one experimental group. Control group 1 was cultured with α-MEM (Gibco, 12000-022) supplemented with 10% fetal bovine serum (FBS) (Gibco), 1X penicillin / streptomycin (Gibco), and control group 2 And the experimental group was supplemented with 10% fetal bovine serum (FBS) (Gibco), 1X penicillin / streptomycin (Gibco), 40ng / mL human nuclear factor κ-B ligand receptor activator (receptor activator of nuclear factor kappa-B ligand, RANKL) (Peprotech, 310-01) and 25ng / mL human monocyte colony stimulating factor (M-CSF) (Peprotech, 300-25) α -MEM (Gibco, 12000-022). All three groups of cells were cultured on 6-well plates with a concentration of ¹⁰⁶ cells / well. The cells in the experimental group were additionally added with an extract of 0.25 mg / mL jackfruit white core. After 24 hours of culture, the cell cultures of each group were collected. And used for gene expression analysis.

In this embodiment, the genes used to analyze the differentiation related to osteoclasts include the CFOS gene, the colony stimulating factor 1 receptor (CSF1R) gene, and the RANK gene; Genes include spleen tyrosinase kinase (SYK) gene and tartrate resistant acid phosphatase (TRAP) gene.

The RNA extraction kit (Geneaid) was used to extract RNA from each group of cell cultures obtained above. Each group whereby RNA obtained and taken to 2,000ng SuperScript ^® III reverse transcriptase ^{(SuperScript ® III Reverse Transcriptase) (} Invitrogen) The extracted RNA was reverse transcribed to cDNA. Next, cDNA was used as a template, and primer pairs [including CFOS, CSF1R, RANK, SYK, TRAP, and RPLP0 (as internal control)] were used to amplify the target genes. Their nucleotide sequences are shown in Table 1 below]. The KAPA CYBR FAST qPCR Kit (2x) (KAPA Biosystems) was used in the StepOne Plus Real Time PCR system (ABI) for quantitative real-time PCR. polymerase chain reaction (hereinafter referred to as quantitative real-time PCR), in order to amplify and quantify the target gene. The melting curve of the PCR product was confirmed during the quantitative real-time PCR reaction.

Relative expression levels of the target gene is derived from equations 2 ^- _△△ ^Ct, using RPLP0 gene (as an internal control) and the cycle threshold level gene by standard deviation and the relative fold change is calculated, wherein △ Ct = Ct _{of target genes / reference gene} -Ct _RPLP0, △△ Ct = △ Ct _{gene of interest} - △ Ct _{reference gene} fold change = 2 ^- _△△ ^Ct _average. The target gene expression in the control group 1 was used as the comparison benchmark of 1. The statistically significant difference between the groups was determined by the single-tailed Student's t-test. The results of this example are shown in FIGS. 2 and 3.

Figure 2 is a data chart of the efficacy of the jackfruit white core extract of the present invention in regulating the expression of genes related to osteoclast differentiation (including CFOS gene, CSF1R gene and RANK gene); Figure 3 is the jackfruit white core extract of the present invention A data map of the efficacy of substances in regulating the expression of genes (including SYK gene and TRAP gene) related to bone resorption of osteoclasts. It can be seen from FIG. 2 that whether the CFOS gene, the CSF1R gene, or the RANK gene is compared with the control group 1, the relative expression of genes related to osteoclast differentiation is measured in the control group 2, which indicates that Osteo-erosion cells can be differentiated. Compared with the control group 2, the experimental group measured that the relative expression of genes related to osteo-osteocyte differentiation decreased. This indicates that the extract of jackfruit white core can indeed inhibit bone erosion. Cell Differentiation. It can be seen from FIG. 3 that, in terms of the TRAP gene, compared with the control group 1, the relative expression of genes related to bone resorption of the osteoclasts measured by the control group 2 has been increased, which indicates that the osteoclasts can carry bone. Absorption, whether it is SYK gene or TRAP gene, compared with control group 2, the experimental group measured a significant reduction in the relative expression of genes related to bone resorption of osteoclasts, which indicates that jackfruit white core extract It can indeed inhibit bone erosion by bone erosion cells. The results of this experiment show that the extract of jackfruit white core of the present invention can achieve the effect of improving osteoporosis by negatively regulating the expression of genes related to osteoclast differentiation and bone resorption.

Example 3. Evaluation of the effectiveness of jackfruit white core extract on cardiovascular health

In this example, the applicant explores whether the extract of jackfruit white core can achieve the effects of cardiovascular health by regulating the expression of genes related to HDL production and cholesterol metabolism.

First, HepG2 cells were cultured in a 6-well culture plate in a DMEM medium (Gibco, 12100-038) supplemented with FBS (Gibco, 10437-028) and penicillin / streptomycin (Gibco, 15140122) at a cell concentration of 1 × 10 ⁵ cells / well, followed by incubation at 37 ° C for 24 hours, and the medium was removed.

Thereafter, the cultured cells were divided into two groups, including a control group and an experimental group. The extract of jackfruit white core obtained in Example 1 was diluted with a medium to a diluent having a concentration of 0.25 mg / ml, and then added to the cells of the experimental group, and the cells of the control group were added to the medium. Next, the cell cultures of each group were collected and used for gene expression analysis.

In this embodiment, genes used to analyze HDL production and cholesterol metabolism include apolipoprotein A-1 (APA1) gene and ATP binding cassette subfamily A member 1 ABCA1) gene.

RNA extraction was performed on each of the cell cultures obtained in the RNA extraction kit. 2,000 ng of each group of RNA thus obtained was reverse transcribed into cDNA using SuperScript ^® III reverse transcriptase. Next, use cDNA as a template and use primer pairs [including APA1, ABCA1, and β-actin (as an internal control group) to amplify the target genes. Their nucleotide sequences are shown in Table 2 below], use the KAPA CYBR FAST qPCR kit (2x) to perform quantitative real-time PCR in the StepOne Plus real-time PCR system to amplify and quantify the target gene. The melting curve of the PCR product was confirmed during the quantitative real-time PCR reaction.

Relative expression levels of the target gene is derived from equations 2 ^- _△△ ^Ct, and the use of β- actin gene (as an internal control group) and the reference gene threshold cycle and to calculate the relative standard deviation by fold change, wherein △ Ct = Ct _{target gene / gene} - Ct _{reference [beta] -actin,} △△ Ct = △ Ct _{gene of interest} - △ Ct _{reference gene} fold change = 2 ^- _△△ ^Ct _average. The target gene expression in the control group was used as the comparison benchmark of 1. Statistically significant differences between groups were determined by the one-tailed Stuart's t-test. The results of this example are shown in FIG. 4.

FIG. 4 is a data chart of the efficacy of the jackfruit white core extract of the present invention in regulating the expression of genes related to HDL production and cholesterol metabolism (including APA1 gene and ABCA1 gene). It can be seen from FIG. 4 that whether it is APA1 gene or ABCA1 Compared with the control group, the relative expression of genes related to HDL production and cholesterol metabolism in the experimental group was significantly improved. Results of this example It has been shown that the extract of jackfruit white core of the present invention can achieve cardiovascular health care effects by positively regulating the expression of genes related to HDL production and cholesterol metabolism.

Example 4. Evaluation of the effectiveness of jackfruit white core extract on improving hot flashes

In this embodiment, the applicant explores whether the extract of jackfruit white core can achieve the effect of improving hot flashes by regulating the expression of genes related to the inflammation response and apoptosis of endothelial cells.

First, HUVEC cells were cultured in a 6-well culture plate in Medium 200 medium (Gibco, M-200-500) supplemented with LSGS (Gibco, S-003-10) and penicillin / streptomycin (Gibco, 15140122). The concentration was 1 × 10 ⁵ cells / well, followed by incubation at 37 ° C. for 24 hours, and the medium was removed.

Thereafter, the cultured cells were divided into two groups, including a control group and an experimental group. The jackfruit white core extract obtained in Example 1 was diluted with a medium to a diluent having a concentration of 0.25 mg / mL, and then added to the cells of the experimental group, and the cells of the control group were added to the medium. After 48 hours of effect, cell cultures from each group were collected and used for gene expression analysis.

In this embodiment, the genes used to analyze the inflammation response of endothelial cells include the interleukin-6 (IL-6) gene and the vascular cell adhesion molecule 1, VCAM1 gene; and Endothelial cell apoptosis-related genes are caspase 8, CASP8 gene.

RNA extraction was performed on each of the cell cultures obtained in the RNA extraction kit. 2,000 ng of each group of RNA thus obtained was reverse transcribed into cDNA using SuperScript ^® III reverse transcriptase. Next, use cDNA as a template and use primer pairs [including IL-6, VCAM1, CASP8, and β-actin (as an internal control group) to amplify the target gene. Their nucleotide sequences are shown below Table 3]. The KAPA CYBR FAST qPCR kit (2x) was used for quantitative real-time PCR in the StepOne Plus real-time PCR system to amplify and quantify the target gene. The melting curve of the PCR product was confirmed during the quantitative real-time PCR reaction.

Relative expression levels of the target gene is derived from equations 2 ^- _△△ ^Ct, and the use of β- actin gene (as an internal control group) and the reference gene threshold cycle and to calculate the relative standard deviation by fold change, wherein △ Ct = Ct _{target gene / gene} - Ct _{reference [beta] -actin,} △△ Ct = △ Ct _{gene of interest} - △ Ct _{reference gene} fold change = 2 ^- _△△ ^Ct _average. The target gene expression in the control group was used as the comparison benchmark of 1. Statistically significant differences between groups were determined by the one-tailed Stuart's t-test. The results of this example are shown in FIG. 5.

FIG. 5 is a data chart showing the efficacy of the jackfruit white core extract of the present invention in regulating the expression of genes associated with endothelial cell inflammation and apoptosis (including IL-6 gene, VCAM1 gene and CASP8 gene), as can be seen from FIG. 5 Regardless of the IL-6 gene, VCAM1 gene or CASP8 gene, compared with the control group, the relative expression of genes related to the inflammation response and apoptosis of endothelial cells was significantly reduced in the experimental group. The results of this example show that the extract of jackfruit white core of the present invention can achieve the effect of improving hot flashes by negatively regulating the expression of genes related to the inflammation response of endothelial cells and apoptosis.

Example 5. Evaluation of the effectiveness of jackfruit white core extract in improving insomnia

In this example, the applicant explores whether the extract of jackfruit white core can achieve the effect of improving insomnia by regulating the expression of genes related to tryptophan metabolism.

First, SH-SY5Y cells were cultured in a 6-well culture plate in a DMEM medium (Gibco, 12100-038) supplemented with FBS (Gibco, 10437-028) and penicillin / streptomycin (Gibco, 15140122) at a cell concentration of 1 × 10 ⁵ cells / well, followed by incubation at 37 ° C for 24 hours, and the medium was removed.

Thereafter, the cultured cells were divided into two groups, including a control group and an experimental group. The jackfruit white core extract obtained in Example 1 was diluted with a medium to a diluent having a concentration of 0.25 mg / mL, and then added to the cells of the experimental group, and the cells of the control group were added to the medium. After 48 hours of effect, cell cultures from each group were collected and used for gene expression analysis.

In this embodiment, genes used to analyze tryptophan metabolism include the DOPA decarboxylase (DDC) gene and Acetylserotonin O-methyltransferase-like protein, ASMTL protein) gene.

RNA extraction was performed on each of the cell cultures obtained in the RNA extraction kit. 2,000 ng of each group of RNA thus obtained was reverse transcribed into cDNA using SuperScript ^® III reverse transcriptase. Next, use cDNA as a template and use primer pairs for amplification of the target gene [including DDC, ASMTL, and GAPDH (as an internal control group), their nucleotide sequences are shown in Table 4 below], immediately in StepOne Plus In the PCR system, the KAPA CYBR FAST qPCR kit (2x) was used for quantitative real-time PCR, and the target genes were amplified and quantified. The melting curve of the PCR product was confirmed during the quantitative real-time PCR reaction.

Relative expression levels of the target gene is derived from equations 2 ^- _△△ ^Ct, and the use of β- actin gene (as an internal control group) and the reference gene threshold cycle and to calculate the relative standard deviation by fold change, wherein △ Ct = Ct _{target gene / reference gene} -Ct _GAPDH, △△ Ct = △ Ct _{gene of interest} - △ Ct _{reference gene} fold change = 2 ^- _△△ ^Ct _average. The target gene expression in the control group was used as the comparison benchmark of 1. Statistically significant differences between groups were determined by the one-tailed Stuart's t-test. The results of this example are shown in FIG. 6.

FIG. 6 is a data chart of the efficacy of the extract of jackfruit white core according to the present invention in regulating the expression of genes related to tryptophan metabolism (including DDC gene and ASMTL gene). It can be seen from FIG. 6 that both the DDC gene and the ASMTL gene Compared with the control group, the relative expression of genes related to tryptophan metabolism measured in the experimental group was significantly improved. The results of this example show that the extract of jackfruit white core of the present invention can increase the production of serotonin and melatonin by positively regulating the expression of genes related to tryptophan metabolism, thereby achieving the effect of improving insomnia.

In summary, the extract of jackfruit white core of the present invention can indeed regulate the CFOS gene, CSF1R gene, RANK gene, SYK gene, TRAP gene, APA1 gene, ABCA1 gene, IL-6 gene, VCAM1 gene, CASP8 gene, DDC gene And the expression of ASMTL protein genes, and achieve improvement by inhibiting osteoclast differentiation and bone resorption, promoting HDL production and cholesterol metabolism, reducing endothelial cell inflammation and apoptosis, and increasing serotonin and melatonin production. Effects of menopausal syndromes (including osteoporosis, cardiovascular disease, hot flushes, and insomnia).

The above description is exemplary only, and not restrictive. Any equivalent modification or change made without departing from the spirit and scope of the present invention shall be included in the scope of the attached patent application.

<110> Dajiang Biomedical Co., Ltd.

<120> Jackfruit white core extract is used to regulate CFOS gene, CSF1R gene, RANK gene, SYK gene, TRAP gene, APA1 gene, ABCA1 gene, IL-6 gene, VCAM1 gene, CASP8 gene, DDC gene, and ASMTL protein Gene expression and use for improving menopause syndrome

<130> 107B0158-I1

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Claims (11)

An extract of white core of Artocarpus heterophyllus is used to prepare a gene that regulates CFOS gene, colony stimulating factor 1 receptor (CSF1R) gene, RANK gene, and spleen tyrosinase kinase (SYK) ) Gene, tartrate resistant acid phosphatase (TRAP) gene, apolipoprotein A-1 (APA1) gene, ATP binding cassette subfamily A member 1, ABCA1) gene, interleukin-6 (IL-6) gene, vascular cell adhesion molecule 1 (VCAM1) gene, apoptotic protease 8 (caspase 8, CASP8) gene, DOPA decarboxylase (DOPA decarboxylase, DDC) gene and the expression amount of Acetylserotonin O-methyltransferase-like protein (ASMTL protein) gene for pharmaceutical use, wherein the jackfruit white core is extracted The jackfruit white core was extracted by using water as an extraction solvent. The use as described in item 1 of the patent application range, wherein the liquid-solid ratio of the extraction solvent to the jackfruit white core is between 2 ~ 10: 1 ~ 5. The use according to item 1 of the patent application range, wherein the extraction temperature is between 50 ° C and 100 ° C. The use according to item 1 of the patent application range, wherein the extraction time is between 0.5 and 3 hours. The use according to item 1 of the patent application scope, wherein the pharmaceutical product comprises a pharmaceutically acceptable carrier. An extract of jackfruit ( Artocarpus heterophyllus ) white core is used for preparing a medicine for improving menopause syndrome, wherein the extract of jackfruit white core is obtained by extracting the jackfruit white core with water as an extraction solvent. The use as described in item 6 of the scope of patent application, wherein the liquid-solid ratio of the extraction solvent to the jackfruit white core is between 2 ~ 10: 1 ~ 5. The use according to item 6 of the patent application range, wherein the extraction temperature is between 50 ° C and 100 ° C. The use according to item 6 of the patent application, wherein the extraction time is between 0.5 and 3 hours. The use according to item 6 of the scope of patent application, wherein the amenorrhea syndrome includes osteoporosis, cardiovascular disease, hot flushes, and insomnia. The use as described in claim 6 of the scope of patent application, wherein the pharmaceutical product comprises a pharmaceutically acceptable carrier.