TWI634912B - Washing and sterilizing method - Google Patents

Abstract

The present invention relates to a method for cleaning and sterilizing an object including a medical instrument, an animal medical device, a meat processing tool, and a cooking utensil, comprising: an alkaline electrolyzed water washing step using alkaline electrolyzed water for the first specific Ultrasonic cleaning or agitation washing of the object in time; and an acidic electrolyzed water washing step, after the alkaline electrolyzed water washing step, the object is treated with acidic electrolyzed water for a second specific time Wash or impregnate with stirring.

Description

Washing and sterilizing method

The present invention relates to a method of cleaning and sterilizing a subject attached to a medical instrument including a medical steel tool, an optical medical device, a medical synthetic resin device, an animal medical device, a meat processing tool, and a cooking utensil. . Specifically, the present invention relates to a method in which a blood-derived organic substance derived from a living body is attached to an object and is spread (infected) by a microorganism, a virus, and a Prion protein (PrP ^sc ) attached to the object. The amyloid protein is washed, and the organic matter adhering to the object is decomposed, and the propagation (aggregation) of the amyloid protein (PrP ^sc ) adhering to the object is suppressed, and the adhesion is suppressed. The microorganism is sterilized, and the virus attached to the object is not activated. More specifically, the present invention relates to an industrial oil which is attached to a medical device such as a ceramic or metal artificial joint, a metal intramedullary nail, a fixing pin, a fixing plate, a screw, or the like, and the like. Or the removal of organic matter, as well as the cleaning, disinfection, sterilization, sterilization, and virus inactivation of microorganisms attached to the surface. Further, in particular, the present invention relates to the formation of a hydroxyl radical which is irradiated with ultrasonic waves of water or alkaline electrolyzed water, and the cleaning, disinfection, sterilization, sterilization, and virus of the pollutants using the reaction. Activation, prion protein (PrP ^sc ) and transmission (infectious) amyloid transmission and aggregation inhibition methods.

A method for cleaning and sterilizing a camera or other peripheral device attached to an endoscope or an endoscope is disclosed in Patent Document 1 below. The cleaning and sterilizing method described in Patent Document 1 below is a method of dividing a diaphragm having electrodes on both sides. The undissolved alkaline electrolyzed water production tank is used as a washing tank, and the endoscope camera apparatus or the like inserted into the washing tank is pre-washed by ultrasonic cleaning with water, and then supplied to the electrolytic tank. The alkaline electrolyzed water produced by electrolysis of the sodium chloride aqueous solution is ultrasonically washed, and then the alkaline electrolyzed water in the alkaline electrolyzed water production tank is discharged, and the acidic electrolyzed water in the acidic electrolyzed water production tank is transferred to the alkali. The electrolytic water is sprayed and sterilized in the production tank, and after being washed with water, it is dried by applying warm air to the washing tank. In addition, in the following Patent Document 1, the endoscope camera device that has been ultrasonically washed with alkaline electrolyzed water is washed and washed with water, and then washed and disinfected with acidic electrolyzed water. .

In the present application, the term "washing" refers to the removal of contaminants adhering to an object from an object. In addition, the term "contaminant" refers to an organic substance such as blood, body fluid, fat, prion protein (PrP ^sc ), and a protein or cell tissue such as a spread (infectious) amyloid, a microorganism, a virus, or the like. Moreover, "disinfection" means that microorganisms or viruses adhering to an object are reduced. Further, "sterilization" refers to the decomposition and extinction of pathogenic microorganisms. Moreover, "sterilization" means that the pathogenic microorganisms adhering to the object are reduced to ^10-6 before the treatment. Further, "inactivation" refers to loss of viral infectivity, inhibition of transmission (infection) and aggregation of Prion protein (PrP ^sc ), spread (infectious) amyloid.

[Previous Technical Literature] [Patent Literature]

[Patent Document 1] Japanese Patent No. 4359284

The method of washing and sterilizing described in Patent Document 1 is effective in removing common protein contamination and Prion protein (PrP ^sc ), but causes the transmission of Prion (PrP ^sc ) and transmitted (infectious) amyloid to be lost. Sexuality and inhibition of the inactivation of cohesion cannot achieve sufficient effects. Further, in order to prevent the inability to re-aggregate and spread (infect) the pulverized protein (PrP ^sc ) and the spread (infected) amyloid protein after washing, sufficient effects cannot be exerted.

The present invention has been made in view of such a problem, and an object thereof is to provide a method for removing and decomposing protein contamination of prion protein (PrP ^sc ) and propagating (infectious) amyloid, and suppressing inclusion in a washing liquid. A virus in which the decomposed amyloid is re-aggregated and has a propagating state, so that it loses the force of adhesion to equipment or utensils, including medical instruments, animal medical devices, meat processing tools, and cooking utensils. Lyon protein and a method of invigorating (infectious) amyloid.

In order to solve the above problems, the cleaning and sterilizing method of the present invention is a method for cleaning and sterilizing an object including a medical device, an animal medical device, a meat processing tool, and a cooking utensil, comprising: an alkaline electrolytic water washing step And ultrasonic cleaning or stirring and washing the object in the first specific time by the alkaline electrolyzed water; and an acidic electrolysis water washing step, after the alkaline electrolysis water washing step, using the acidic electrolysis The object is immersed or stirred and washed in the second specific time.

According to the present invention, since the alkaline electrolyzed water and acidic electrolyzed water is suitable for cleaning respective particular time, it is possible to remove prion protein (PrP ^sc) and amyloid-like propagation (infection) of protein contamination, and It loses cohesion and prevents the spread of Prion (PrP ^sc ) and amyloid (infection). Moreover, according to the cleaning and sterilizing method of the present invention, even if the medical instrument, the animal medical device, the meat processing tool, and the cooking utensil as the object are not exposed to a high-temperature and high-pressure environment, a sufficient washing effect can be obtained. Since the transmission of Prion (PrP ^sc ) and amyloid (infection) is prevented, it can be used even for medical instruments, animal medical devices, meat processing tools, and cooking utensils that cannot withstand high temperature and high pressure.

Further, in the cleaning and sterilizing method of the present invention, it is also preferred that the first specific time ratio is The second specific time mentioned above is long.

In a preferred embodiment, the effect of ultrasonic cleaning or stirring washing by alkaline electrolyzed water can be surely ensured by sufficiently ensuring the first specific time, thereby allowing the use of Prion protein (PrP ^sc ) or the like. The spread (infectious) of the spread (infectious) amyloid is not activated, but inhibits aggregation. As a result, the time of immersion in the acidic electrolyzed water can be shortened, and the influence of the acidic electrolyzed water on the optical precision medical device or the influence of the chloride on the metal object can be reduced.

Further, in the cleaning and sterilizing method of the present invention, it is also preferred to stir the acidic electrolyzed water in the acidic electrolyzed water washing step.

In this preferred embodiment, the efficiency of washing sterilization and virus inactivation can be further improved by stirring the acidic electrolyzed water, and on the other hand, the Prion protein (PrP ^sc ) and the spread (infectious) amyloid can be lost. On the other hand, the time for immersing the object in the acidic electrolyzed water can be further shortened, and the influence of the acidic electrolyzed water on the optical precision medical device or the influence of the chloride on the metal object can be reduced.

Further, in the cleaning and sterilizing method of the present invention, preferably, the method further comprises: a water washing step of washing the object with water after the acidic electrolytic water washing step; and an ultraviolet irradiation step, wherein After the water washing step, the object is irradiated with ultraviolet rays.

In the preferred embodiment, after the acidic electrolytic water washing step, the acidic electrolyzed water or the product produced by the acidic electrolyzed water can be removed by the water washing step, thereby reducing the acid electrolyzed water to the optical precision medical treatment. The influence of the device or the influence of the chloride on the metal object, and the floating bacteria in the air can be prevented by the ultraviolet irradiation step, thereby maintaining the state after the washing.

Further preferably, the method further includes a water washing step of ultrasonically washing or agitating the object with water for a third specific time before the washing and sterilizing step of the present invention. .

In this preferred embodiment, the object can be ultrasonically washed or stirred and washed with water to shorten the time required for the subsequent alkaline electrolyzed water washing step and the acidic electrolyzed water washing step. Thereby, damage to the optical precision medical device caused by cavitation can be suppressed.

Further, in the cleaning and sterilizing method of the present invention, it is preferable that the alkaline electrolyzed water and the acidic electrolyzed water are obtained by electrolysis of an aqueous sodium chloride solution or an aqueous potassium chloride solution supplied to the electrolytic cell.

In this preferred embodiment, alkaline electrolyzed water and acidic electrolyzed water can be obtained by electrolysis of an aqueous solution of sodium chloride or potassium chloride, and alkaline electrolyzed water and acidic electrolyzed water having higher detergency can be obtained. Used for washing.

Further, when sodium carbonate or potassium carbonate is added to sodium chloride or potassium chloride in an aqueous solution of sodium chloride or potassium chloride for about 10 to 50%, and carbonic acid is produced by sodium carbonate and potassium carbonate. The buffer effect of the root ions is suppressed in the pH of the acidic electrolyzed water, and the pH value of the alkaline electrolyzed water is increased by the increase of the sodium ion or the potassium ion, and the whole body tends to the alkaline side, thereby suppressing the electrolysis. Chlorine gas is generated, so it is also preferable in terms of environmental protection.

According to the present invention, it is possible to provide a medical device which can remove protein contamination such as prion protein and spread (infectious) amyloid, and which can inactivate virus, prion protein and spread (infectious) amyloid, A method for cleaning and sterilizing medical devices, meat processing tools, and cooking utensils for animals.

Fig. 1 is a view showing the results of generation of radical species.

Fig. 2A is a view showing the results confirmed by the RT-QUICK method in the case where a 5 mm stainless steel wire of a brain emulsion is not attached.

Fig. 2B is a graph showing the results confirmed by the RT-QUICK method in the case of attaching 2 μl of a 5 mm stainless steel wire of 0.01% brain emulsion.

Fig. 2C is a graph showing the results confirmed by the RT-QUICK method by attaching 2 μl of a 5 mm stainless steel wire to a 0.1% brain emulsion.

Fig. 2D is a graph showing the results confirmed by the RT-QUICK method by attaching 2 μl of a 5 mm stainless steel wire to 1% of a brain emulsion.

Fig. 2E is a graph showing the results confirmed by the RT-QUICK method in the case of attaching 2 μl of a 5 mm stainless steel wire to 10% of a brain emulsion.

Fig. 2F is a graph showing the results confirmed by the RT-QUICK method after the 5 mm stainless steel wire + washing step (3 min) to which 2 μl of 0.01% brain emulsion was attached.

Fig. 2G is a graph showing the results confirmed by the RT-QUICK method in the case of a 5 mm stainless steel wire + washing step (3 min) to which 2 μl of a 0.1% brain emulsion was attached.

Fig. 2H is a graph showing the results confirmed by the RT-QUICK method after attaching 2 μl of a 1% brain emulsion to a 5 mm stainless steel wire + washing step (3 min).

Fig. 2I is a graph showing the results confirmed by the RT-QUICK method by attaching 5 μm of a 5 mm brain emulsion to a 5 mm stainless steel wire + washing step (3 min).

Fig. 3A is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3B is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

FIG 3C are diagrams caused by the concentration of NaOH RT-QUICK (Instant Fast) method of human prion protein (PrP ^sc) does not activate the evaluation results of FIG.

Fig. 3D is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3E is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3F is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3G is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

FIG. 3H are diagrams caused by the concentration of NaOH RT-QUICK (Instant Fast) method of human prion protein (PrP ^sc) does not activate the evaluation results of FIG.

Fig. 3I is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3J is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3K is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3L is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3M is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3N is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3O is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3P is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3Q is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3R is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

Fig. 3S is a graph showing the results of evaluation of the inactivation of human prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method.

FIG 3T are diagrams caused by the concentration of NaOH RT-QUICK (Instant Fast) method of human prion protein (PrP ^sc) does not activate the evaluation results of FIG.

Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings. For the sake of easy understanding, the same components are denoted by the same reference numerals throughout the drawings, and the description thereof will not be repeated.

Prior to the description of the embodiments of the present invention, the spread (infection) of amyloid fibers such as Prion protein (PrP ^sc ) will be described. Proteins in living organisms exert their functionality by forming a unique three-dimensional structure.

The protein may employ the same amino acid component and the alpha structure and the beta structure which are arranged in a steric manner. When a protein functioning by the α structure changes to a β structure, its function is lost. The free energy of the β structure is lower than that of the α structure, and the protein of the α structure is stabilized by the β structure of a lower energy state. Therefore, a protein that functions as an alpha structure has a function of maintaining its structure. For example, a disulfide bond of a covalent bond obtained by coupling two groups of thiols is also one of them.

The formation of amyloid fibers, which are called causes of numerous diseases, will be described below. First, the alpha structure of a particular protein is converted to a beta structure (reaction 1). When the alpha structure is converted to the beta structure, the hydrophobic amino acid located in the hydrophobic structure within the molecule is exposed on the surface of the protein molecule (Reaction 2). By exposing the hydrophobic amino acid to the surface of the protein molecule, the entropy is increased, and the exposed hydrophobic amino acids undergo hydrophobic-hydrophobic interaction, dipolar-dipolar interaction, and electrostatic interaction with each other. By these interactions, the β strands containing the hydrophobic amino acid overlap each other in the vertical direction of the molecular axis to form a βsheet, and the βsheets are mutually interacted by hydrogen bonds or the like (reaction 3). Thus, the industry believes that amyloid precursor proteins will aggregate.

Further, the industry believes that when the amyloid precursor protein aggregates, it begins to be called amyloid fibrosis. The test results of hydrazine exchange revealed that the hydrogen bond of the bonded amyloid protein gradually became stronger, and the bond strength was equivalent to crystallization (reaction 4). Thus, the crystallized amyloid converts the alpha structure of the normal amyloid precursor protein into a beta structure. This is seeding, although it is usually a phenomenon of "PrP ^sc ", but it is different from the infection of bacteria.

The seed phenomenon of the crystallization reaction is a reversible reaction in a direction in which the free energy (G) is reduced, and is known as a Gibbs reaction. The following is its reaction formula.

△G=△E+△PV-△ST (Formula 1)

G: Free energy

S: Entropy

T: temperature

E:焓

P: pressure

V: volume

The driving force for crystallization is based on (Formula 1), but the entropy (s) per one particle is obtained by the following (Formula 2).

s=S/N= μ(T,P)/ T (Formula 2)

s: entropy per 1 particle

S: Entropy

N: total number of particles

δ: partial differential symbol

μ: chemical potential

As a result of the increase in the entropy of (Reaction 2) and the reaction of (Formula 2), the reaction proceeds in the direction in which the free energy of (Formula 1) is lowered, so that the driving force for βstranding and crystallization can be explained.

The reaction process in which the protein changes from the α structure to the β structure and is fibrotic is utilized in the reaction of the driving force shared by amyloid formation, and the process is promoted by seeding. That is, it can be said that the amyloid protein is infectious when the amyloid precursor protein is amyloidized by seeding.

Although the industry believes that amyloid other than Prion protein (PrP ^sc ) is not infectious in organisms, it has recently been reported that the cause of Parkinson's disease is infection with amyloid. Synuclein. Science 16 November 2012: Vol.338 no.6109 pp.949-953.

Compared with other amyloid proteins, Prion protein (PrP ^sc ) has a strong hydrogen bond and is difficult to decompose by protease. Therefore, it is considered that the Prion protein (PrP ^sc ) which adheres to food and invades from the digestive organs is not decomposed in the digestive organs and is absorbed into the body to become a seed, thereby causing infection. However, when other amyloid proteins do not decompose and invade into the body, for example, surgical instruments or non-disposable medical instruments from the time of surgery are more likely to be infected.

As a method effective for the inactivation of Prion protein (PrP ^sc ), there is a method of incinerating an object contaminated with Prion protein (PrP ^sc ) (previous method 1); and placing it in an autoclave at 134 ° C for 1 hour ( Previous method 2); boiled for 10 minutes at 100 ° C with 3% SDS (Sodium dodecyl sulfate, sodium dodecyl sulfate) solution (previous method 3); immersed in 7M guanidine hydrochloride solution for 2 hours at room temperature (previous Method 4); immersed in 3 M guanidine thiocyanate solution for 2 hours at room temperature (previous method 5); immersed in 3 M trichloroacetic acid solution for 2 hours at room temperature (previous method 6); at room temperature Immersed in 60% formic acid solution for 2 hours (previous method 7); immersed in 50% phenol solution for 2 hours at room temperature (previous method 8); immersed in 1% sodium hypochlorite solution for 2 hours at room temperature, and in the chamber It was immersed in 1N sodium hydroxide for 1 hour under temperature (previous method 9) and the like. However, no matter by what method, were unable to avoid the need to damage the appliance inactivate prion protein (PrP ^sc) of.

The cleaning and sterilizing method of the present embodiment is different from the methods of the prior methods 1 to 9, and is a medical device that cannot withstand high-pressure, high-temperature or high-concentration drugs, and can be used to realize prion protein ( The PrP ^sc ) and the spread (infected) amyloid are washed and removed, and the method of washing and sterilizing which causes the propagation (infection) activity caused by amyloidization is lost. Specifically, it includes an alkaline electrolyzed water washing step of ultrasonically washing or stirring the object such as a medical instrument, a meat processing tool, or a cooking utensil to be washed by alkaline electrolyzed water; And an acidic electrolytic water washing step, after the alkaline electrolytic water washing step, the object is immersed in acidic electrolyzed water and stirred. Further, if necessary, a water washing step may be included, which is performed before the alkaline electrolytic water washing step, using water (purified water, water suitable for drinking, distilled water, pure The object and the water passing through the filter are ultrasonically washed or stirred and washed.

In the first step (water washing step), the surface of the tap water (purified water, distilled water, pure water, water passing through the filter) may be subjected to ultrasonic irradiation. Shoot, or wash the object while stirring the tap water. The purpose of the first step is to improve the cleaning efficiency of the second step described below when a large amount of contaminants adhere to the object. When the adhesion of the contaminant on the object is relatively slight, the first step may be omitted.

The washing time in the first step (water washing step) is preferably from 1 to 3 minutes, more preferably from 3 to 5 minutes, and still more preferably from 5 to 10 minutes. There is a tendency that the washing effect is higher when the washing time in the first step is 3 minutes or longer. Further, there is a tendency that the cleaning efficiency in the first step is about 3 minutes, and the cleaning efficiency is higher. Moreover, it is preferable that the washing time of the first step is changed depending on the amount of the object and the degree of contamination of the object.

In the second step, ultrasonic irradiation is performed on the alkaline electrolyzed water, or the object is washed for 3 minutes while stirring the alkaline electrolyzed water. The purpose of this second step in that the contaminants adhering to the cleaning object is removed, leaving the prion protein (PrP ^sc), spread (infection) and amyloid inactivated virus. By generating ultrasonic radicals by ultrasonic irradiation in alkaline electrolyzed water, the cell wall of the microorganism as a contaminant, the inactivation of the prion protein (PrP ^sc ) and the spread (infectious) amyloid, and aggregation inhibition are realized. The virus is decomposed and inactivated, and organic matter such as fat from a living body as a pollutant is decomposed.

The washing time in the second step (alkaline electrolyzed water washing step) is preferably from 1 to 3 minutes, more preferably from 3 to 5 minutes, still more preferably from 5 to 20 minutes. There is a tendency that the washing effect is higher when the washing time in the second step is 3 minutes or longer. Further, there is a tendency that the cleaning efficiency in the second step is about 3 minutes, and the cleaning efficiency is higher. Moreover, it is preferable that the washing time of the second step is changed depending on the amount of the object to be cleaned and the degree of contamination of the object.

As the pH of the alkaline electrolyzed water, the pH is preferably about 11.5 to 12. Since the inhibitory effect of the transmission (infection) and aggregation of the Prion protein (PrP ^sc ) and the spread (infectious) amyloid depends on the concentration of the hydroxide ion, by using such alkaline electrolyzed water, Prion protein (PrP ^sc ) and the spread (infectious) of amyloid-producing (infectious) inactivation and aggregation inhibition, the washing effect is further improved, and the washing is removed by decomposition of organic matter from the living body. The effect is improved.

Further, before the second step (alkaline electrolyzed water washing step), an aqueous solution of sodium chloride or potassium chloride is added to an electrolysis device separated by a separator to perform electrolysis, and an acidic solution (acidic electrolysis) is obtained on the anode side. Water), and an alkaline solution (alkaline electrolyzed water) was obtained on the cathode side.

After the alkaline electrolyzed water used in the second step is discharged from the washing tank, in the next third step, the water impregnated with the object (the purified water may be suitable) Ultrasonic irradiation is carried out with drinking water, distilled water, pure water, water passing through the filter, or the object is preferably washed for 1 minute while stirring the water. The purpose of the third step is to prevent re-adhesion of residual contaminants or decomposed contaminants after the second step, so that the residual contaminants that cannot be removed in the first and second steps are peeled off and removed from the object by the object. The water is washed with alkaline electrolyzed water. Furthermore, the third step may or may not be performed as appropriate.

Thereafter, in the fourth step (acidic electrolytic water washing step), the acidic electrolyzed water is stirred, and it is preferably washed for 3 minutes or more depending on the type of the object and the amount of the contaminated deposit. The purpose of the fourth step is to achieve washing, disinfection, sterilization, sterilization, propagation inactivation of prion protein (PrP ^sc ) and spread (infectious) amyloid, aggregation inhibition by stirring acidic electrolyzed water. The virus is not activated to sufficiently obtain the washing, disinfecting, and sterilizing effects of bacteria such as acid-fast bacteria and spore-forming bacteria which are strongly resistant to external influences. HOCl (molecular hypochlorous acid) in acidic electrolyzed water penetrates the cell wall and plasma membrane of the bacteria, and the enzyme or nucleic acid inside the bacteria is oxidized, so that the cells are extinct.

The washing time in the fourth step (acidic water washing step) is preferably from 1 to 3 minutes, more preferably from 3 to 5 minutes, still more preferably from 5 to 10 minutes. There is a tendency that the washing effect is higher by the washing time in the fourth step of 3 minutes or longer. Further, the cleaning time in the fourth step can be about 3 minutes, and the cleaning can be performed efficiently.

As the pH of the acidic electrolyzed water, the pH is preferably about 2.5. There is a tendency to further improve the washing effect by using such acidic electrolyzed water. In order to consider the working environment and suppress the amount of chlorine gas generated, the pH is preferably 2.5 to 3.5.

Thereafter, in the fifth step (water washing step), tap water impregnated with the object (the purified water may be water suitable for drinking, distilled water, pure water, water passing through the filter) Ultrasonic irradiation is performed, or the object is washed for 1 minute while stirring the tap water. The purpose of the fifth step is to remove and remove the acidic electrolyzed water or the decomposition residue used in the fourth step from the object, thereby improving the cleanliness of the object. In the case of a metal object, it is preferable to set the time of the fifth step to be longer than the fourth step.

Then, in the sixth step (ultraviolet irradiation step), the object is dried by supplying clean air such as a filter, and the object is irradiated with ultraviolet rays. By the sixth step, the floating bacteria mixed in the air can be prevented, and the state after washing can be maintained.

In the case of the present embodiment, it is preferred that the time for performing the second step (alkaline electrolyzed water washing step) is longer than the time for performing the fourth step (acidic electrolyzed water washing step). For example, the washing with alkaline electrolyzed water is preferably between 3 and 20 minutes depending on the amount of the raw material, the amount of the object to be washed, and the amount of the attached contaminant, and is washed by the acidic electrolyzed water. The amount of the raw material, the amount of the object, and the amount of the attached contaminant is preferably between 1 minute and 20 minutes. In this way, the effect of ultrasonic cleaning by alkaline electrolyzed water and agitation washing with acidic electrolyzed water can be surely achieved, and the Prion protein (PrP ^sc ) and the spread (infectious) amyloid protein are not activated. Prevent its spread (infection) and coagulation.

Further, in the present embodiment, the preferred embodiment includes the steps of: water used in the first step, the third step, and the fifth step, and alkaline electrolyzed water used in the second step, and the fourth step The acidic electrolyzed water used in the mixture is mixed, and the mixed waste liquid is electrolyzed. Thereby, sterilization of the bacteria washed out from the object and inactivation of the virus, and inactivation of the prion protein and the spread (infectious) amyloid and aggregation inhibition can be expected.

In addition, in other steps than the fourth step (acidic electrolytic water washing step), depending on the degree of contamination of the object and the degree of adhesion of the contaminant, or depending on the object such as the endoscope camera or the endoscope body For the type, ultrasonic cleaning or agitation washing may be selected, or both of the cleaning methods may be selected. However, cavitation caused by ultrasonic cleaning may cause concern about optical precision medical instruments such as metal objects, endoscopes, and endoscope cameras. Therefore, in the case of selecting ultrasonic cleaning, it is preferable to intermittently or continuously illuminate the ultrasonic waves in accordance with the shape of the object to be washed, the adhesion strength of the surface and the internal cavity contaminants. Further, the irradiation time of the ultrasonic wave is preferably 10 to 30% of the cleaning time of the first step, depending on the shape of the object and the cleaning conditions specified by the object.

Here, in the other steps than the fourth step (acidic electrolysis water washing step), the washing is performed by only the agitation washing without ultrasonic cleaning, and the ultrasonic cleaning is performed simultaneously. The washing and washing were carried out while stirring, and the time required for removing the bacteria from the object was evaluated. Specifically, a plurality of test bodies in which Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and nontuberculous mycobacteria are attached to the same extent are prepared, and each test body is washed by washing only with stirring, and At the same time, ultrasonic cleaning and stirring washing were performed to wash, and the time required for each bacteria to be removed to a practical level was evaluated. The evaluation results are shown in Table 1. The time is the time required for each of the second step and the fourth step. The first step is 3 minutes, and the third and fifth steps are 1 minute.

It is confirmed that in the other steps than the fourth step (acidic electrolysis water washing step), the ultrasonic cleaning and the stirring are simultaneously washed and washed (with ultrasonic waves). Compared with the case where the ultrasonic cleaning is not performed and the cleaning is performed only by the agitation washing (no ultrasonic waves), the time required for each step can be substantially shortened.

Further, comparative information of radical species generated by a mixed solution of ultrapure water, sodium chloride aqueous solution, and alkaline electrolyzed water irradiated with ultrasonic waves is shown in Fig. 1. Specifically, the results are as follows: (a) a mixed solution of 800 μL of ultrapure water and 200 μL of 890 mM DMPO solution, (b) a mixed solution of 800 μL of a 4% aqueous sodium chloride solution and 200 μL of a 890 mM DMPO solution, (c) 800 μL of alkaline electrolyzed water (pH = 11.6) and 200 μL of 890 mM DMPO solution were mixed into a microtube of 1500 μL and irradiated for 10 minutes for comparison. Base species.

As shown in Fig. 1, it was confirmed that a radical species was formed in all the mixed solutions irradiated with ultrasonic waves, but it was confirmed that a large number of radical species were formed particularly in the mixed solution of alkaline electrolyzed water. Among the radical species, hydroxyl radicals are particularly fast in reaction and are known as intermediates for promoting oxidation reactions. Further, it is known that a hydroxyl radical causes a protein modification or an enzyme inactivation reaction. Therefore, as in the second step, in the step of washing with alkaline electrolyzed water, it is possible to obtain a higher washing effect, prion protein and spread (infection by irradiation of ultrasonic waves in alkaline electrolyzed water). Inactivation of amylopectin and inhibition of aggregation.

(Example)

In the example, the needle to which the prion protein (PrP ^sc ) was attached was washed according to the above-described cleaning and sterilizing method, and then the stainless steel needle was inserted into the brain parenchyma of the mouse, and the survival until the onset of death was measured. time. As a control, the unwashed needle was inserted into the brain parenchyma of the mouse, and the survival time until the onset of death was measured. The washing-up prion protein (infection) verification procedure used in this example is described for each step.

(Step 1) exposing the stainless steel needle (diameter 0.2mm) onset of the disease in the brain prion protein (PrP ^sc) (Fukuoka-1 strains LD50 / g = 107.9) of 50% brain homogenate for 10 minutes, and dried at room temperature 1 hour.

(Step 2) The prion protein (PrP ^sc )-contaminated needle prepared in the step 1 was inoculated directly into the mouse brain (depth 5 mm, 3 seconds) without washing.

(Step 3) prepared in Step 1 of the prion protein (PrP ^sc) tap water contaminated needle ultrasonic cleaning for 20 minutes, and inoculated directly into the brain of mice (the depth of 5mm, 3 seconds).

(Step 4) The prion protein (PrP ^sc )-contaminated needle prepared in the step 1 was ultrasonically washed with an alkaline electrolyte for 20 minutes, and directly inoculated into the mouse brain (depth of 5 mm, 3 seconds).

(Step 5) The prion protein (PrP ^sc )-contaminated needle was placed in acidic electrolyzed water, washed while stirring for 10 minutes, and directly inoculated into the mouse brain (depth 5 mm, 3 seconds).

In the sample group 1, the steps 1 and 2 were carried out, and the prion protein (PrP ^sc )-infected needle was not washed and inoculated into the mouse brain. After the specimen in group 2, step 1 and step 3, and the tap water of prion protein (PrP ^sc) contaminated needle ultrasonic cleaning for 20 minutes, the needle inoculated into the brain of mice. In the sample group 3, steps 1, 3, and 4 were carried out, and the Prion (PrP ^sc )-stained needle was ultrasonically washed with tap water for 20 minutes, and ultrasonic cleaning was performed for 20 minutes using alkaline electrolyzed water. The needle was inoculated into the brain of the mouse. In the sample group 4, steps 1, 3, 4, and 5 were carried out, and the Prion (PrP ^sc )-stained needle was ultrasonically washed with tap water for 20 minutes, and then subjected to ultrasonication for 20 minutes using alkaline electrolyzed water. After washing, it was immersed in acidic electrolyzed water for 10 minutes, and then the needle was inoculated into the mouse brain. The respective washing effects are shown in Table 2.

According to the results of the sample group 3, it was found that three of the four mice were not affected by the washing of the needle and the ultrasonic wave and the washing of the alkaline electrolyzed water and the ultrasonic wave by the Prion protein (PrP ^sc ). . According to the results of the sample group 4, it was found that the alkaline electrolyzed water attached to the needle of the Prion protein (PrP ^sc ) was washed with ultrasonic waves and stirred with acidic electrolyzed water, and none of the five mice were observed. Onset.

Further, it is assumed that the prion protein (PrP ^sc ) contained in the washing solution after the needle is attached to the prion protein (PrP ^sc ) is subjected to an infection test of Prion protein (PrP ^sc ). The Prion protein (PrP ^sc ) which is detached from the needle by ultrasonic washing or immersion and stirring is mixed into the solution, but the presence or absence of the seeding activity is not clear. Therefore, the infectivity of the Prion protein (PrP ^sc ) contained in the drain after washing was verified.

(Comparative experiment) 10 ml of physiological saline after washing 6 prion protein (PrP ^sc ) contaminated needles was centrifuged and concentrated at 10,000 g for 1 h, dissolved in 1 mL, and 0.02 mL was directly inoculated into the mouse brain (depth) 5mm, 3 seconds).

(Step 6) 10 mL of alkaline electrolyzed water, which was washed with 6 prion protein (PrP ^sc ) contaminated needles used in the step 4, was centrifuged at 10,000 g for 1 h, and then dissolved to 1 mL, and taken directly at 0.02 mL. Inoculated in the brain of mice (depth 5 mm, 3 seconds).

(Step 7) 10 mL of acidic electrolyzed water which was washed with 6 prion protein (PrP ^sc ) contaminated needles used in the step 5 was centrifuged and concentrated at 10,000 g for 1 hour, and then dissolved into 1 mL, and 0.02 mL was directly inoculated. Mouse brain (depth 5mm, 3 seconds).

The sample group 5 was carried out (comparative experiment). In the sample group 6, steps 1 and 6 are performed, and in the sample group 7, steps 1 and 7 are performed. Table 3 shows the results of an infection evaluation test using a waste liquid after washing a needle contaminated with Prion protein (PrP ^sc ).

In this way, the alkaline electrolyzed water and the acidic electrolyzed water discharged by washing the needle to which the Prion protein (PrP ^sc ) was attached were applied to the needle, and the cells were inoculated into the mouse brain, respectively, and the infection was confirmed. The rate is reduced.

Such a result reveals that the trait is attached to the needle by the prion protein (PrP ^sc) of a difference, the surface of the release from the needle, in addition by exposure to acidic electrolyzed water and alkaline electrolyzed water, prion protein (PrP ^sc The infectivity may change.

The infection of the Prion protein (PrP ^sc ) refers to Seeding. The decrease in the free energy caused by the decrease in the entropy caused by the molecular linkage accompanying the polymerization will advance the Seeding by the Prion protein (PrP ^sc ) molecule from the α structure to the β structure.

The presence of a charged amino acid in the amyloidogenic core of amyloidogenic protein forming amyloid is surrounded by the hydrophobic environment of the protein in a natural state, but when it changes from an alpha structure to a beta structure, It becomes a hydrophilic environment and undergoes electrostatic interaction to produce amyloidization (βsheetization), which progresses to fibrosis. However, it is known that Asp (Aspartate, aspartic acid) and Glu (Glutamic acid, glutamic acid) lose negative charge due to positively charged hydrogen ions in an acidic, especially hydrogen-rich, strongly acidic environment. (Nature 1996 382: 180-182).

In the alkaline environment of OH ^- rich, the negatively charged OH ^- ion electrons surround the amyloid-forming amino acid molecule, and the charge of the surface of the positively charged amino acid changes and loses the bonding function. Therefore, the polymerization of Prion protein (PrP ^sc ) is hindered.

Therefore, in an environment where the residual chlorine concentration in the alkaline electrolyzed water, the acidic electrolyzed water, and the acidic electrolyzed water is 20 to 80 ppm, the Seeding of the Prion protein (PrP ^sc ) is not generated, and the infectivity is lost. The results confirmed by the RT-QUICK method are shown in Fig. 2. The prion protein (PrP ^sc ) was attached to a stainless steel wire, and the washing effect was confirmed by an RT-QUICK method. Fig. 2A is a view showing the result of the RT-QUICK method for a steel wire to which no substance is adsorbed. 2B to E are views showing the results of the RT-QUICK method on the steel wire to which the homogenization is adsorbed. 2F to 1I are diagrams showing the results of the RT-QUICK method of the steel wire to which the brain homogenate is adsorbed after being washed by the washing and sterilizing method of the present embodiment.

Further, the evaluation results of the inactivation of human Prion protein (PrP ^sc ) by the NaOH concentration of the RT-QUICK method are shown in Figs. 3A to T. In Figs. 3A to T, "sCJD" is "sporadic Creutfeldt Jakob disease". The vertical axis of the table indicates the ThT (Thioflavin T) fluorescein intensity, and the horizontal axis indicates the number of cycles. Brain Homogenate is a brain lotion. “0N NaOH” means “0 equivalent sodium hydroxide”, “0.01N NaOH” means “0.01 equivalent sodium hydroxide pH 11.3”, “0.1N NaOH” means “0.1 equivalent sodium hydroxide pH 12.6”, “1N NaOH” "It means "1 equivalent of sodium hydroxide pH 13.2". "SD50/g Brain" means "SD50 is Standard Deviation, 50% lethal dose".

In the case of bacteria: in the acidic electrolyzed water environment containing hydrogen-rich ions (H+) of residual chlorine, the bond equilibrium of the negatively charged phospholipids of the cell membrane forming the pathogenic bacteria is destroyed by the oxidizing ability, resulting in destruction of the cell membrane. The internal cytoplasm flows out, making the bacteria unable to sustain life. The bacteria are killed and lose their infectivity.

Table 4 shows the bactericidal effect when washing with alkaline electrolyzed water and acidic electrolyzed water obtained by adding sodium chloride and performing electrolysis.

The oxidation reaction caused by H+ acts on the cell wall of the virus, DNA (Deoxyribonucleic acid), RNA (Ribonucleic acid, ribonucleic acid), so that the virus is not activated.

Claims (5)

A method for cleaning and sterilizing, comprising a method for cleaning and sterilizing a medical device, an animal medical device, a meat processing tool, and a cooking utensil, comprising: an alkaline electrolytic water washing step using alkaline electrolyzed water Ultrasonic cleaning or agitation washing of the object in the first specific time; acidic acid water washing step, after the alkaline electrolyzed water washing step, using acidic electrolyzed water in the second specific time The object is subjected to stirring washing or immersing; a water washing step of washing the object with water after the acidic electrolytic water washing step; and an ultraviolet irradiation step, after the water washing step, The object is irradiated with ultraviolet rays. A method for cleaning and sterilizing, comprising a method for cleaning and sterilizing a medical device, an animal medical device, a meat processing tool, and a cooking utensil, comprising: an alkaline electrolytic water washing step using alkaline electrolyzed water Ultrasonic washing or stirring washing of the object in the first specific time; and an acidic electrolyzed water washing step, after the alkaline electrolyzed water washing step, using the acidic electrolyzed water in the second specific time And stirring or immersing the object; and further comprising a water washing step of using the water to perform the object in the third specific time before the alkaline electrolyzed water washing step Wash or wash with sonic waves. A method for cleaning and sterilizing, comprising a method for cleaning and sterilizing a medical device, an animal medical device, a meat processing tool, and a cooking utensil, comprising: an alkaline electrolytic water washing step using alkaline electrolyzed water In the first specific time The object is subjected to ultrasonic cleaning or agitation washing; and an acidic electrolyzed water washing step, after the alkaline electrolyzed water washing step, the object is stirred and washed in the second specific time by the acidic electrolyzed water. The above-mentioned alkaline electrolyzed water and the above-mentioned acidic electrolyzed water are obtained by electrolysis of an aqueous solution of sodium chloride or an aqueous solution of potassium chloride supplied to the electrolytic cell. The method of washing and sterilizing according to any one of claims 1 to 3, wherein the first specific time is longer than the second specific time. The method of washing and sterilizing according to any one of claims 1 to 3, wherein the acidic electrolyzed water is stirred in the acidic electrolytic water washing step.