TWI595235B - Method for evaluating rough skin - Google Patents

Description

Rough skin assessment method

The present invention relates to a method for evaluating rough skin.

The skin of the human body is often in contact with the outside world and is subject to various stimuli. Its representative stimulus is ultraviolet light. Even if ultraviolet rays do not cause a large change in the appearance of the skin, DNA lysis of skin cells and denaturation of collagen may be induced to cause skin aging. Ultraviolet light constantly exerts pressure on the skin. For women, daily routines such as face washing and makeup can also cause irritation to the skin. When the external stimulus of the skin accumulates, it is considered to be a state in which the skin is rough. Although the skin is rough, it refers to the symptoms of the moment, but the state in which the symptoms persist is called "rough skin."

"Rough skin" refers to a general term for a state in which dry, dry skin symptoms persist, and its most prominent clinical manifestation is scaly. There is a close relationship between this rough skin condition and the barrier function of the skin. It is known that the more severe the rough skin condition, the higher the transepidermal water evapotranspiration (hereinafter referred to as TEWL) as an objective indicator of the barrier function, as the physical/ The index of the rough state of the skin caused by the main factors of chemistry is measured by TEWL (Non-Patent Document 1: Nikko, Vol. 92, pp. 1001 - 1003, 1982).

The rough skin is considered to be ultraviolet rays, chemicals, and other various substances as described above. A variety of stimuli affect the internal environment of the skin, resulting in inhibition of the barrier function of the epidermis. When such a condition occurs on the skin, the following changes occur, and the concentration of prostaglandins and cytokine which affect cell proliferation/differentiation inside the skin increases (Non-Patent Document 2: J. Invest. Dermatol, Vol. 81, 519-523, 1983), the advancement of nucleic acid (DNA) synthesis function (Non-Patent Document 3: Journal of Clinical Investigation, Vol. 87, 1668-1673, 1991), TEWL rises closely related to barrier function (Non-patent literature) 4: J. Lipid. Res., Vol. 26, 418-427, 1985, Vol. 30, 323-333, 1989) and the like. However, there are few reports on the relevance of these phenomena, and the detailed mechanisms are not clear. Therefore, the indicators of rough skin have so far relied on my own declaration and visual judgment, and there are no clear indicators.

In addition, there are people with a potentially rough skin condition, but it is difficult to detect such a rough skin that is not apparent on the surface. One of the skin conditions of this potentially rough skin can also be called so-called sensitive skin.

The evaluation of the usefulness of the active ingredient in the external preparation for skin such as the cosmetics and the quasi drug is based on one screening by an in vitro test, two screening by an experiment using an animal, It is carried out in the order of use test of the human body. However, in the case where the mechanism such as rough skin is complicated and the main reason cannot be specified, it is very difficult to carry out an in vitro biological test focusing on the steps of participating in various mechanisms, and the final evaluation can only be performed on the human body with the degree of rough skin as an indicator. On the test, the indicators at this time are in any case mainly based on sensory evaluation and non-quantitative methods such as macroscopic observation and microscopic observation (microscopic observation).

In recent years, some proposals have been made to solve the above problems. A method of measuring the degree of coloration by contacting the skin with an alcohol solution of diphenylpicrylsulfonyl radical (DPPH) is proposed in Patent Document 1 (JP-A-H11-76168). patent A method of using a squamous cell carcinoma-associated antigen of the stratum corneum of the skin as an index is disclosed in Document 2 (International Publication No. 2006/098523). Further, Patent Document 3 (International Publication No. 98/40045) discloses a method in which the amount of change in the degree of order of the dermal collagen fiber bundle is used as an index. Further, a method of extracting pyrrolidone carboxylic acid from the skin with water and evaluating the amount of extraction with water is proposed in Patent Document 4 (JP-A-2010-71917).

The present inventors have discovered a new function of galectin-7 which has not been noticed in the past, and is a proposal for utilizing this function for the first time. Galectin refers to a generic term for identifying a protein that binds between galactose (β-galactoside structure) or a crosslinked sugar chain. Life phenomena such as participation, differentiation, morphogenesis, tumor metastasis, and apoptosis (cell death) were elucidated. Currently, galectin which is known as a mammal has 15 types of galectin-1 to -15. In the case of the human body, there is no galectin which is equivalent to -5, -6, -14, -15, and there are currently 10 kinds, and there is a possibility of further increase in the future. Galectin is roughly classified into three subgroups from its morphology. The first subtype has one part that binds to a sugar chain (sugar chain binding domain 2: a proto-type of a carbohydrate recognition domain (CRD)), and the second subtype is a sugar The strand-binding domain has a chimera-type of a structure that does not bind to a sugar chain and is linked to other domains, and the third subtype is a tandem-repeat formed by two sugar-chain-binding domains (tandem-repeat) -type). Proto-type and chimera-type have only one sugar chain-binding domain, but are bound by two molecules (forming a dimer), and substantially in engagement with (tandem-repeat-type) is the same as binding to two sugar chains.

Galectin-7 was detected from the stratified epithelium of mammals, and galectin-7 was detected from basal layer to stratum corneum in keratinocytes. The stratified epithelium is distributed in the epidermis, esophageal epithelium, squamous epithelial tracheal epithelium, external cervical epithelium, hypopharyngeal to larynx epithelium, anal epithelium, and the like. Since the expression of galectin-7 in keratinocytes is not affected by the concentration of Ca ²⁺ , it is known that it is not controlled by the degree of keratinization (Non-Patent Document 5: Differetiation, 1998, 63, 159-168) . On the other hand, the expression in fibroblasts was not confirmed. Further, it is also known that galectin-7 is weakly reduced by retinoic acid (Non-Patent Document 6: Developmental Biology 168, 259-271 (1995)).

It has been reported that galectin-7 is induced by p53 (cancer suppressor gene) and designated as PIG1 (P53-induced gene 1), which is involved in apoptosis. The activation of galectin-7 is enhanced by the activation of caspase-3 and the cleavage of poly(ADP-ribose) polymerase (Non-Patent Document 7: J Biol Chem. 2002 Feb 1; 277(5): 3487-97.Epub 2001 Nov 8.). It is known that when keratinocytes are irradiated with UVB, the expression of galectin-7 is sharply increased, and apoptosis of keratinocytes is caused by the JNK-BaX pathway (Non-Patent Document 8: Proc Natl Acad Sci US A. 1999) Sep 28; 96(20): 11329-34).

Further, in the patent document 5 (JP-A-2006-182744), a screening method of a galectin-7 expression promoting substance and a moisturizing action by a composition for applying a galectin-7 component are disclosed. And skin symptoms improve. Further, an external preparation containing one or two or more types of galectin has been disclosed to prevent and improve aging symptoms such as wrinkles and elasticity of the skin and wound healing effects, as disclosed in Japanese Laid-Open Patent Publication No. Hei 9-216833. .

[Previous Technical Literature] [Patent Literature]

[Patent Document 1] Japanese Patent Laid-Open No. Hei 11-76168

[Patent Document 2] International Publication No. 2006/098523

[Patent Document 3] International Publication No. 98/40045

[Patent Document 4] Japanese Patent Laid-Open Publication No. 2010-71917

[Patent Document 5] Japanese Patent Laid-Open Publication No. 2006-182744

[Patent Document 6] Japanese Patent Laid-Open Publication No. Hei 9-216833

[Non-patent literature]

[Non-Patent Document 1] Nikko, Vol. 92, pp. 1001 - 1003, 1982

[Non-Patent Document 2] J. Invest. Dermatol, Vol. 81, 519-523, 1983

[Non-Patent Document 3] Journal of Clinical Investigation, Vol. 87, 1668-1673, 1991

[Non-Patent Document 4] J. Lipid. Res., Vol. 26, 418-427, 1985, Vol. 30, 323-333, 1989

[Non-Patent Document 5] Differetiation, 1998, 63, 159-168

[Non-Patent Document 6] Developmental Biology 168, 259-271 (1995)

[Non-Patent Document 7] J Biol Chem. 2002 Feb 1; 277(5): 3487-97. Epub 2001 Nov 8.

[Non-Patent Document 8] Proc Natl Acad Sci U S A. 1999 Sep 28; 96(20): 11329-34

As described above, an objective evaluation method for evaluating the degree of rough skin is expected. The present invention is a method for non-surgically extracting skin without applying irritation to the skin, and the object of the present invention is to provide a simple and reliable assessment of whether it is rough skin. A new assessment method for biochemical indicators.

The present invention has the following constitution.

(1) A method for evaluating the degree of rough skin, which is an amount of expression of galectin-7 in the stratum corneum of the skin.

(2) A method for evaluating the degree of rough skin, which compares the expression amount of galectin-7 in the stratum corneum of the skin with the expression amount of galectin-7 in the skin of the normal site measured in advance or simultaneously. .

(3) A method for evaluating sensitive skin, which compares the expression amount of galectin-7 in the stratum corneum of the skin with the expression amount of galectin-7 in the skin of the normal portion measured in advance or simultaneously.

(4) The evaluation method according to (1) or (2), comprising: a step of collecting the stratum corneum of the skin, and measuring the expression amount of galectin-7 in the stratum corneum cells collected in the collecting step. The measurement step is a comparison step of comparing the expression amount of galectin-7 measured in the measurement step with the expression amount of galectin-7 in the stratum corneum of healthy normal skin.

(5) The evaluation method according to (4), wherein the step of collecting the stratum corneum of the skin is performed by a tape peeling method.

According to the evaluation method of the present invention, the degree of rough skin can be evaluated by measuring the expression amount of galectin-7 in the stratum corneum. In addition, the amount of galectin-7 expressed as an indicator can easily detect potentially rough skin. Further, since the evaluation method of the present invention collects the evaluation sample by a simple operation method such as a tape peeling method, the burden on the subject is small, and anyone can easily evaluate it. Further, since it is a biochemical test method, the same result can be obtained regardless of the measurement. Therefore, it is not necessary to consult with an expert as in the past.

Further, the effects of cosmetics and the like can be objectively evaluated by the method of the present invention.

Fig. 1 is a graph showing the amount of expression of galectin-7 in each part of the human body.

Fig. 2 is a graph showing the amount of expression of galectin-7 in a forced rough skin model produced by SDS.

Fig. 3 is a graph showing the amount of expression of galectin-7 in a forced rough skin model made of acetone.

Fig. 4 is a graph showing the expression and frequency of galectin-7 of 608 healthy and normal skin.

Fig. 5 is a graph confirming the correlation between galectin-7 and TEWL.

Fig. 6 is a graph showing changes in the expression amount of galectin-7 before and after 14 days of use of a moisturizing cream.

Fig. 7 is a graph showing changes in the TEWL value and the water content of the stratum corneum before and after 14 days of use of a moisturizing cream.

Hereinafter, the present invention will be described in detail.

The evaluation method of the present invention is characterized by the amount of galectin-7 present in the stratum corneum of the skin as an index.

The rough skin in the present invention refers to a skin which continuously exhibits symptoms of scales in a dry, dry state. In addition, sensitive skin refers to a skin that has no obvious skin lesions but is prone to adverse and harmful reactions, and is called a skin with potentially rough skin. And it is less resistant to external irritations than healthy normal skin. It can also be said to be a skin that is prone to skin problems. On the other hand, healthy normal skin means not showing as above The healthy and normal skin of the rough skin and sensitive skin. It is known that the TEWL is increased in sensitive skin, and the high-frequency conductivity (the water content of the stratum corneum) is lowered. The skin, known as sensitive skin, sometimes causes dermatitis and rough skin.

The stratum corneum is the outermost layer of the skin and has the function of protecting the skin from foreign bodies and irritation from outside the body.

The site to be evaluated in the present invention may include any portion as long as it is a site in which the stratum corneum layer is obtained, but the main site includes a face, a neck, and an upper arm. According to the conventional method, the stratum corneum of the skin from the above-mentioned site is obtained. However, as described above, since the method of surgically removing the skin or the like imposes a burden on the user, a method of easily obtaining a stratum corneum such as tape peeling or scratching is preferred.

The amount of galectin-7 present in each sample thus prepared can be measured by a conventionally known method. For example, a method such as an enzyme immunoassay, a radioimmunoassay, or a Western blot method based on the reaction of an antibody against galectin-7 can be used. Such a measurement method is commercially available, and a Galectin 7 Human ELISA Kit manufactured by Abcam and an ELISA Kit manufactured by R&D Systems Inc. can be exemplified as a representative enzyme immunoassay kit for galectin-7.

From each of the samples, the galectin-7 was prepared by a biochemical method known per se, such as a freeze-thaw method, a sonication method, a homogenization method, or the like. And measured quickly after extraction.

Since the expression of galectin-7 in the stratum corneum is significantly higher than that of healthy and normal skin, the amount of galectin-7 in the stratum corneum can be easily evaluated. The accumulation of pressure or the skin is rough skin. The stratum corneum used in the present invention can be collected by the following simple method, that is, only the surface layer portion of the stratum corneum is collected with a keratin tape as in the tape peeling method. And this The invention can measure the expression amount of galectin-7 without stimulating the skin with ultraviolet rays, chemicals or the like. Therefore, it is possible to evaluate whether the user's skin is rough or not without burdening the user. In particular, it is possible to easily select a cosmetic or chemical rejuvenating agent that is more suitable for the skin of the user, thereby avoiding skin problems such as skin inflammation, side effects, and further exerting the skin care effect of the cosmetic.

The evaluation method of the present invention comprises the step of collecting the stratum corneum, the measuring step of measuring the expression amount of galectin-7 in the stratum corneum collected in the above-mentioned collecting step, and the galectin measured in the aforementioned measuring step. A comparison step of comparing the amount of expression of -7 with the amount of expression of galectin-7 in the stratum corneum of healthy normal skin. The evaluation method will be described below.

First, the expression amount of galectin-7 measured as described above was compared with the expression amount of galectin-7 in the stratum corneum of the healthy skin of the subject itself. Further, the expression amount of galectin-7 in the stratum corneum of a certain evaluation target site of the subject is measured, and the measured amount of galectin-7 measured is the same as that of a person with healthy normal skin. The amount of expression of galectin-7 in the stratum corneum of the subject site was compared. The expression level of galectin-7 in the stratum corneum of a person with healthy normal skin can be more objectively evaluated by using the average value of data of a plurality of people with healthy normal skin. More specifically, the distribution of the expression level of galectin-7 by a randomly extracted subject sets the distribution of healthy normal skin.

As a result of such comparison, in the case where the measured amount of galectin-7 was significantly greater than that of galectin-7 in healthy normal skin, it was evaluated that the accumulation of pressure was large, even if rough skin was not present. It can also be judged as a possibility of being sensitive skin. This healthy and normal skin sample can be measured from galactose The skin collected from the human body of glycoside-7 can also be collected from the skin of a person different from the person who measures galectin-7.

In addition, the expression level of galectin-7 in the stratum corneum of a certain evaluation target site of the subject is significantly larger than that of the galectin-7 in the stratum corneum of the same evaluation target site of a person with healthy normal skin. In the case of the amount of performance, it is also estimated that the accumulation of pressure is large. Moreover, even if the surface is not rough skin, it is evaluated as having sensitive skin when the value is remarkably high.

That is, in the case where it is evaluated that the pressure accumulation is high, the degree of pressure accumulation can be evaluated based on the degree of expression of the measured galectin-7. When the measured amount of galectin-7 measured was significantly higher than that of galectin-7 in healthy normal skin, the skin was evaluated to strongly exhibit the properties of rough skin. Moreover, compared with healthy normal skin, the resistance to external stimuli is remarkably low, and it is judged that it is highly likely to cause skin problems. In addition, when the amount of galectin-7 measured is only slightly increased compared with the amount of galectin-7 in healthy normal skin, the skin is more stressed than normal normal skin, The resistance to external stimuli is low, and although the possibility of skin problems is low, it can be evaluated as having the possibility of being mildly sensitive.

The specific embodiments are described below, but the present invention is not limited to the following embodiments.

[Examples] 1. Determination of the expression of galectin-7 in various parts of the human body <subject>

Samples were taken from the cheeks, the medial forearm, and the buttocks of 35 males with healthy and normal skin (including those with previous atopic dermatitis (AD)).

<Stromal layer sample extraction method, protein quantification>

The stratum corneum was collected by adhering a stratum corneum test piece (ASAHIBIOMED) to the subject's cheeks, arms, and buttocks. From the stratum corneum test piece, use the glass bead method (the method of placing the analyte in the container, the glass bead with a diameter of about 2 mm and the extract for shaking extraction) using 500 μl of T-PER extraction buffer (Tissue Protein Extraction) Reagent) The product of the stratum corneum protein was produced by Thermo Scientific (product # 78510). The amount of protein in each sample was measured using a Pierce BCA Protein Assay Kit (Thermo Scientific #23225). At the time of measurement, 200 μl of a reaction solution of Pierce BCA protein Assay Kit was added to 10 μl of the aforementioned stratum corneum protein extract, and after incubation at 60 ° C for 30 minutes, the absorbance at 562 nm was measured. At the same time, a calibration curve was drawn using BSA, and the amount of protein was calculated from the value of the absorbance.

<Measurement of Galectin-7 in the stratum corneum>

Similarly, the amount of galectin-7 was measured using a ELISA kit (galectin-7: DY1339E) manufactured by R&D Systems Co., Ltd. from the stratum corneum protein extract extracted from the tape. First, in a 96-well microtiter plate (#3590) manufactured by Costar, the immobilized antibody which is a primary antibody was immobilized at a concentration specified by a kit at 20 ° C overnight. Next, the plate was sealed with 1% BSA at 37 ° C for 1 hour, and the plate was washed with 0.01% Tween-PBS, and 100 μl of each of the standard samples and samples which became calibration curves were used, and incubated at 25 ° C for 2 hours. After washing the plate with 0.01% Tween-PBS, the antibody was incubated for 2 hours at 25 ° C using the specified concentration of the kit. Further, after washing the plate with 0.01% Tween-PBS, Streptavidin-HRP was incubated at 25 ° C for 30 minutes using the specified concentration of the kit. Finally, after washing the plate with 0.01% Tween-PBS, the chromogenic substrate TMB was dissolved. The solution (Promega, G7431) was added at 100 μl/well, and after the specified time of the kit, 100 μl of the stop solution (0.5 M sulfuric acid) was added, and the absorbance at 450 nm was measured to calculate the amount of expression.

<Result>

The measurement results are shown in Fig. 1.

As shown in Fig. 1, it can be confirmed that the expression of galectin-7 is remarkably higher in the cheeks exposed to external stimuli than in the arms and buttocks (buttocks) where the so-called rough skin is not usually present. From the test results, galectin-7 is very useful as an indicator of rough skin and sensitive skin.

2. Changes in the expression of galectin-7 in artificial rough skin model <Production of rough skin model by SDS> <subject>

Fourteen women with healthy normal skin were tested as subjects.

A 10% solution of a surfactant (sodium dodecyl sulfate, SDS below) was applied to the inner side of the left forearm and rubbed back and forth 100 times. Rinse with hot water (35 ° C, 1 L) and wash off the surfactant. As a control, the inner side of the right forearm was also washed with hot water (35 ° C, 1 L). This operation was repeated 4 times a day, and each operation was repeated in a state of being separated by more than 1 hour. This process was continued for 2 days, and a rough skin model was artificially produced.

<horny collection>

Four stratum corneum layers of each portion which were not subjected to SDS washing and subjected to SDS washing were collected in the depth direction. The date of harvesting the stratum corneum was collected on days 4, 8, and 12 before and after washing.

<Spline layer sample extraction method, protein quantification, determination of galectin-7>

The measurement was carried out in the same manner as in the above 1.

<Result>

The individual measurement results of the subjects are shown in Fig. 2. It was confirmed that the measurement results of galectin-7 on the 8th to 12th day after the rough skin preparation treatment were remarkably increased.

<Production of rough skin model by acetone> <subject>

Three women with healthy normal skin were tested as subjects.

The glass funnel (3 cm in diameter) opened up and down was fixed with a rubber band, and a mixture of 2 ml of acetone (WAKO grade) / diethyl ether (WAKO grade) was poured into a funnel with a pipetman and placed on a shaker. Both arms were treated for 20 minutes.

Then, the acetone/diethyl ether mixture in the funnel was removed with a dropper, and tap water was poured therein, and a two-arm treatment (degreasing treatment) was placed on the shaker for 5 minutes. On the next day, the same operation was performed in the same portion to perform degreasing treatment again to produce dry skin. The site treated with tap water alone was used as a control.

<horny collection>

Four cuticles of acetone-free, acetone-treated fraction were collected in the depth direction. The date of collection was collected on the day before acetone treatment, and on days 1, 5, 8, 12, 16, and 20 after acetone treatment.

<Spline layer sample extraction method, protein quantification, determination of galectin-7>

The operation was carried out in the same manner as in the above test method.

<Result>

The individual measurement results of the subjects are shown in Fig. 3. It was confirmed that the measurement results of galectin-7 on the 8th to 12th day after the rough skin preparation treatment were remarkably increased.

From the results of the above rough skin model test, it was confirmed that with the roughness The production of skin, galectin-7 rises.

3. Determination of galectin-7 in healthy and normal skin <subject>

The cheek samples of 608 males with healthy and normal skin (including those with atopic dermatitis) were collected to confirm the distribution of galectin-7. In addition, TEWL, which is an evaluation index of conventional rough skin, was measured for 35 subjects, and it was confirmed whether there was any correlation with the measurement result of galectin-7.

<Stromal layer sample extraction method, protein quantification>

In the same manner as above, a stratum corneum test piece (manufactured by ASAHIBIOMED Co., Ltd.) was adhered to the subject's cheek to collect the stratum corneum.

<Measurement of Galectin-7 in the stratum corneum>

In the same manner as in the above, the amount of galectin-7 was measured using an ELISA kit (galectin-7: DY1339E) manufactured by R&D Systems Co., Ltd. from the stratum corneum protein extract extracted from the tape.

<TEWL value>

TEWL used Delfin, manufactured by Keystone Corporation, to analyze the average of two separate measurements for analysis.

<Result>

The results of the measurement of 608 galectin-7 are shown in Table 1 and Figure 4.

According to Fig. 4, it is shown that 80% of the galectin-7 of the cheek skin of healthy normal skin is within 435 pg/μg. And from the subject’s listening survey, It has been confirmed that a person who has atopic dermatitis in the past, a person who has a feeling of self-feeling and a rough skin, and the like, mostly exhibits galectin-7 of 500 pg/μg or more. Therefore, for example, a measured value of galectin-7 of 500 pg/μg can be used as an index of rough skin.

In addition, the correlation with TEWL is shown in Figure 5.

It can be confirmed that there is a certain correlation between galectin-7 and TEWL.

Judging from the above test results, the measurement of galectin-7 is useful as an indicator of rough skin and can detect potential rough skin that cannot be detected by TEWL.

4. Changes in galectin-7 with improved rough skin <Test content>

Since a person with a high expression level of galectin-7 has a tendency to have a rough skin, a moisturizing cream is applied to a person having a high expression level of galectin-7 (FDR manufactured by Fongi Co., Ltd.) The cream confirmed that the rough skin was improved, the galectin-7 was lowered, and the usefulness of galectin-7 as a rough skin index was confirmed.

<subject>

Ten subjects with a high expression level of galectin-7 (average about 1400 pg/μg protein) were used as subjects.

<Continuous use of cream>

The subject was allowed to apply a moisturizing cream (FDR cream manufactured by Frangipani Co., Ltd.) to the moisturizing cosmetics for daily use in the morning and evening for 14 days.

<Stromal layer sample extraction method, protein quantification>

In the same manner as in the above, the stratum corneum test piece (manufactured by ASAHIBIOMED Co., Ltd.) was used to collect the stratum corneum of the cheeks before and after the continuous use of the moisturizing cream. Same as above 1. Thus, the amount of protein in the stratum corneum was determined.

<Measurement of Galectin-7 in the stratum corneum>

In the same manner as in the above, the keratin layer protein extract extracted from the tape was measured for the amount of galectin-7 by using an ELISA kit (galectin-7: DY1339E) manufactured by R&D Systems.

<TEWL value>

TEWL used Delfin manufactured by Keystone Co., Ltd., and measured the cheeks after washing and drying twice before and after continuous use of the moisturizing cream, and used the average value for analysis.

<The cuticle water content value>

In the stratum corneum water content, the Skicon-200EX manufactured by I.B.S. was used, and the cheeks after washing and drying were measured five times before and after the moisturizing cream was continuously used, and the average value was used for the analysis.

<Result>

The expression level of galectin-7 before and after the continuous use of the moisturizing cream is as shown in Fig. 6, and the TEWL value and the cuticle moisture content are shown in Fig. 7.

The performance of galectin-7 was significantly reduced by continuous use of a 14-day moisturizing cream. On the other hand, the water content of the stratum corneum increased significantly, indicating that the rough skin was improved. There is some reduction in TEWL values, indicating a trend toward improved rough skin.

From the above, it is known that the expression amount of galectin-7 is effective as an indicator of rough skin.

Claims (3)

A method for evaluating the degree of rough skin, which is an evaluation of the expression level of galectin-7 in the stratum corneum of the skin collected by a tape peeling method or a scraping method, and is evaluated when the expression amount is 500 pg/μg or more of total protein. For rough skin. The evaluation method according to claim 1, which comprises the steps of: collecting the stratum corneum of the skin by a tape stripping method or a scraping method, and measuring the galectin in the stratum corneum cells collected in the foregoing collecting step- The measurement step of the expression amount of 7 is a comparison step of comparing the expression amount of galectin-7 measured in the measurement step with the reference value, using 500 pg/μg total protein as a reference value. The evaluation method according to claim 2, wherein the step of collecting the stratum corneum of the skin is performed by a tape peeling method.