TWI546109B - Method for extracting chloropyll - Google Patents

Description

Method for extracting chlorophyll

The present invention relates to a method of extracting chlorophyll.

Chlorophyll is a green pigment in photosynthesis membranes, which is the main component of light captured by photosynthesis. Chlorophyll has several kinds such as a, b, c, d and e. Plants that release oxygen during photosynthesis contain chlorophyll a; chlorophyll b is found in higher plants, green algae and Euglena; chlorophyll c is found in algae, dinoflagellates and brown algae, and chlorophyll d is present in red algae. The chlorophyll in the leaves of higher plants mainly includes chlorophyll a and chlorophyll b. The molecular structure of chlorophyll a is formed by four pyrrole rings through four methylene groups to form a cyclic structure called porphyrin. The center of the porphyrin ring is bonded to one magnesium atom and has a cyclopentanone (V). The propionic acid on the ring IV is esterified with chlorophyll, and the potassium salt formed after saponification is water-soluble. They are insoluble in water and soluble in organic solvents such as ethanol, acetone, diethyl ether, chloroform, etc., so organic solvents can be used to extract chlorophyll from plants.

It is an object of the present invention to provide a method of extracting chlorophyll.

An embodiment of the present invention provides a method for extracting chlorophyll, the method comprising the following steps: Step 1: pulverizing the plant leaf body with liquid nitrogen, the plant body body pulverizing size is 0.7 mm; Step 2: extracting chlorophyll by an organic solvent; Step 3: extracting the chlorophyll-containing liquid, filtering it by a filter paper, and then concentrating the liquid by a thickener; Step 4: collecting the concentrated liquid.

Preferably, in the first step, before the body of the plant leaves is pulverized, the plant leaf body is firstly stored at a low temperature, and dried to evaporate the water in the body of the plant leaf.

Preferably, in the step of pulverizing the plant leaves in the first step, liquid nitrogen is added and pulverized together with the plant leaf body.

Preferably, the organic solvent includes one or more of ethanol, acetone, dimethylformamide, and dimethylhydrazine.

Preferably, in the second step, the extract is placed in a dark room environment and extracted using a centrifuge.

Preferably, in the third step, the filter paper is one or more selected from the group consisting of a qualitative filter paper, a quantitative filter paper, an organic solvent quantitative filter paper, and a glass fiber filter paper.

Preferably, in the third step, the time of the filtering program is controlled so that the filtering program is completed within 24 hours.

Preferably, in the fourth step, the collected concentrated liquid is placed in a refrigerator for storage.

Preferably, in the fourth step, the temperature of the refrigerator is set to 4 degrees Celsius.

The drawings are included to provide a further understanding of the invention, and are incorporated in this specification and constitute a part of this specification. BRIEF DESCRIPTION OF THE DRAWINGS Exemplary embodiments of the invention. In the drawings: Figure 1 is a flow chart of the preservation of plant leaves in a preferred embodiment of the present invention; Figure 2 is a flow chart for extracting and detecting chlorophyll in a preferred embodiment of the present invention; Figure 3 is a comparison of absorption spectra of three organic solutions. Figure.

The embodiments of the present invention are described in detail below with reference to the accompanying drawings and embodiments.

Table 1 is a list of several chlorophyll species in nature. Chlorophyll is a photosynthetic pigment found in plants, algae and cyanobacteria, wherein the pigment is Chlorin. Chlorophyll a and chlorophyll b are ubiquitous in plants commonly found in life. In the present embodiment, terrestrial plants are selected as extracting articles, and the specific steps are as follows.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a flow chart showing the preservation of a plant leaf body in a preferred embodiment of the present invention. As shown in Figure 1 It is indicated that after the leaves of the plant are removed, the work of preserving the fresh water is first carried out, and finally the leaves of the plants are dried to evaporate excess water.

Figure 2 is a flow diagram of the extraction of chlorophyll in a preferred embodiment of the invention. As shown in Fig. 2, in step S1, the pulverizing action of the plant leaf body is performed. The plant leaf body is first obtained and the pretreatment shown in Figure 1 is performed. The body of the plant leaves subjected to the step 1 treatment was soaked with liquid nitrogen, and then the body of the plant leaves was ground into a fine powder having a size of 0.7 mm by means of a pulverizer. The fine powder before the extraction analysis should be stored in a dark and low temperature condition. The pulverizer can use a juice machine and a pulverizer for general foods, but there is a temperature problem during the pulverization process, which affects the experiment. Therefore, liquid nitrogen is added to the body of the plant to be pulverized in the pulverization process of the experiment.

In step two S2, a solution extraction operation of chlorophyll is performed. In the process of extracting chlorophyll, one or more of the following solutions may be used: ethanol, acetone, dimethyldiamine (DMF) and dimethyl hydrazine (DMSO), and the above various solutions are separately added to the ground fine powder. Then, the chlorophyll is extracted by a centrifuge, and in the extraction, the experimental condition is preferably a dark room.

The two solutions of ethanol and acetone are relatively easy to volatilize, and it is very convenient in experiment. Dimethyldiamine and dimethyl hydrazine are harmful to the human body, and it is needed for the extraction of dimethyldiamine and dimethyl hydrazine. Pay attention to the volatilized gas, so additional equipment is needed to discharge the solution gas. The vacuum oven and the exhaust cabinet are mainly used, and the volatilized gas is discharged by the exhaust cabinet. If it is necessary to accelerate the volatilization gas, the vacuum oven can also be used to accelerate the gas discharge by heating and vacuuming.

In the third step S3, the chlorophyll filtration and concentration operation are performed. The extracted liquid, preferably within 24 hours, is filtered by a screening procedure to remove fine particles of the plant leaf body, mainly through filter paper to filter out unwanted portions. Different filter papers have different effects, roughly Divided into qualitative filter paper, quantitative filter paper, organic solution quantitative filter paper and glass fiber filter paper. Filter paper can be selected according to different needs.

To filter out good liquids, use a thickener to concentrate the liquid, but be careful to avoid excessive draining.

In step S4, a save operation is performed. The filtered solution should be placed in a freezer at an optimum temperature of 4 degrees Celsius. The chlorophyll a and chlorophyll b should be detected as soon as possible. If the solution cannot be detected immediately, the solution can be stored at minus 20 degrees Celsius and stored for up to 14 days. It can be stored at minus 70 degrees Celsius and can be stored for up to three times. Months.

A visible light spectrometer can be used to detect the absorption intensity of the chlorophyll a and chlorophyll b wavelengths extracted with different solutions. Figure 3 is a comparison chart of absorption spectra of three solutions, and Table 2 is a graph showing calculation results of chlorophyll concentration in different organic solutions. Where a is a DMF solution, b is an acetone solution, and c is a DMSO solution. As can be seen from the figure, for the chlorophyll extracted from spinach, the characteristic absorption peak of chlorophyll a is near 662 nm, and the peak of absorption of chlorophyll b characteristic is 455 nm. The chlorophyll a concentration, chlorophyll b concentration and total chlorophyll concentration can be calculated by known formulas. The concentration of chlorophyll in the DMF solution was the highest in the three solutions, and the chlorophyll extraction rate was the highest.

Embodiments of the present invention are mainly directed to a portion of a battery front operation, when extracting and manufacturing a battery The chlorophyll is needed, but after the battery is made of chlorophyll, it can be detected by the instrument, and the chlorophyll changes in the battery after the action. For example, the measurement of the PH/ORP (oxidation reduction potential) value detects whether a toxic substance compound is generated during the use of the battery.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes can be made to the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and scope of the present invention are intended to be included within the scope of the present invention.

Claims (9)

A method for extracting chlorophyll, wherein the method comprises the following steps: Step 1: pulverizing the plant leaf body with liquid nitrogen, the plant body is pulverized to a size of 0.7 mm; Step 2: extracting chlorophyll by an organic solvent; Step 3: The chlorophyll-containing liquid is extracted, filtered by a filter paper, and then concentrated by a concentrator; Step 4: The concentrated liquid is collected. The method for extracting chlorophyll according to claim 1, wherein in the step 1, before the body of the plant leaf is pulverized, the plant leaf body is firstly stored at a low temperature, and dried to evaporate water in the body of the plant leaf. The method of extracting chlorophyll according to claim 1, wherein the organic solvent comprises one or more of ethanol, acetone, dimethylformamide, and dimethylhydrazine. The method for extracting chlorophyll according to claim 1, wherein in the second step, the extract is placed in a dark room environment, and the extraction is performed using a centrifuge. The method of extracting chlorophyll according to claim 1, wherein in the step 3, the filter paper is one or more selected from the group consisting of a qualitative filter paper, a quantitative filter paper, an organic solvent quantitative filter paper, and a glass fiber filter paper. The method for extracting chlorophyll according to claim 1, wherein in the third step, the time of the filtering program is controlled so that the filtering program is completed within 24 hours. The method for extracting chlorophyll according to claim 1, wherein in the step 4, the collected concentrated liquid is placed in a refrigerator for storage. The method for extracting chlorophyll according to claim 7, wherein: in the step four, the refrigerator is The temperature is set to 4 degrees Celsius. A method for extracting chlorophyll, wherein the method comprises the following steps: Step 1: pulverizing the plant leaf body, wherein the plant leaf body is pulverized to a size of 0.7 mm; Step 2: extracting chlorophyll by an organic solvent; Step 3: extracting is good The chlorophyll liquid is filtered by filter paper and then concentrated by a concentrator; Step 4: The concentrated liquid is collected.