CN113143979A - Process for extracting champignon liquid - Google Patents

Process for extracting champignon liquid Download PDF

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CN113143979A
CN113143979A CN202110439751.3A CN202110439751A CN113143979A CN 113143979 A CN113143979 A CN 113143979A CN 202110439751 A CN202110439751 A CN 202110439751A CN 113143979 A CN113143979 A CN 113143979A
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bolete
supernatant
muscarinic
heating
ethanol
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桂红珍
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
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    • A61P25/22—Anxiolytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/30—Drugs for disorders of the nervous system for treating abuse or dependence
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    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A—HUMAN NECESSITIES
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps

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Abstract

The invention belongs to the technical field of extraction of muscarinic bacteria liquid, and particularly relates to a muscarinic bacteria liquid extraction process, aiming at the problems that the existing muscarinic bacteria liquid extraction mode is complex to operate and can not meet the use requirement, the following scheme is proposed, which comprises the following steps: s1: preparing a fungus; s2: weighing; s3: grinding; s4: heating; s5: loading into test tube, centrifuging, and mixing the supernatants; s6: heating and concentrating; s7: subpackaging and storing, preparing fresh bolete purchased in the last year in S1, sorting, cleaning, freezing and storing, taking out bolete when in use, unfreezing in a greenhouse, and then drying in the air. The method is convenient to operate, the extraction mode of the muscarinic bacterial liquid is simple, the extraction effect and efficiency of the muscarinic bacterial liquid are improved by crushing and mixing, the supernatant is extracted twice, waste is avoided, the purity of the muscarinic bacterial liquid can be improved by filtering twice, and the use requirement can be met.

Description

Process for extracting champignon liquid
Technical Field
The invention relates to the technical field of extraction of muscarinic bacterial liquid, in particular to a process for extracting muscarinic bacterial liquid.
Background
There are a large number of species in nature, such as: bolete, coprinus, collybia albuminosa, pyrocarbon-type fungi and the like all contain a muscarinic liquid, and actually the bolete is not a virulent strain and can be poisoned only by being processed immature, and the normal liver and kidney functions are not influenced after poisoning, so that neurotransmitter of the brain causes the unreal liver and kidney functions, and the neurotransmitter of the brain causes the unreal, and the venom is extracted from medicine to form pills to treat mental diseases such as poisoning, anxiety, depression and the like.
The existing extraction mode of the muscarinic bacterial liquid is complex to operate and can not meet the use requirement.
Disclosure of Invention
The invention aims to solve the defects that the existing extraction mode of the muscarinic liquid is complex to operate and can not meet the use requirement, and provides a muscarinic liquid extraction process.
In order to achieve the purpose, the invention adopts the following technical scheme:
a process for extracting a mushroom liquid comprises the following steps:
s1: preparing a fungus;
s2: weighing;
s3: grinding;
s4: heating;
s5: loading into test tube, centrifuging, and mixing the supernatants;
s6: heating and concentrating;
s7: subpackaging and storing.
Preferably, in S1, fresh bolete purchased in last year is prepared, sorted, washed, and then frozen for storage, and when in use, the bolete is taken out, placed in a greenhouse for thawing, and then dried.
Preferably, the weight of the mixture is in S2, and three different weight portions of bolete are prepared, wherein the weight of bolete is 70 g, 60 g and 50 g.
Preferably, in S3, the bolete is ground, and screened using a screen, and a plurality of screens and grinding are performed to obtain bolete powder, and the bolete powder is dissolved in ethanol to obtain a bolete solution.
Preferably, in S4, the bolete solution is divided into three parts, the three parts are heated by a microwave oven for 5 minutes, 10 minutes and 15 minutes, respectively, to obtain triple-cooked, quintuple-cooked and fully-cooked bolete solutions.
Preferably, in S5, after three bolete solutions are completely cooled, the bolete solutions are placed into three test tubes, the three test tubes are subjected to rotary centrifugation, the first supernatant is taken, ethanol is added again to be mixed, the centrifugation is performed again, the second supernatant is taken, the two taken supernatants are mixed, and then the mixture is filtered again by using a filter membrane.
Preferably, the S6 is to add three bolete supernatants into a distillation flask separately and heat them at 55-65 deg.C to achieve heating concentration and complete volatilization of ethanol.
Preferably, the S7 records the dose, calculates the concentration, dispenses it, and places it in a freezer for use.
Compared with the prior art, the invention has the advantages that:
the method is convenient to operate, the extraction mode of the muscarinic bacterial liquid is simple, the extraction effect and efficiency of the muscarinic bacterial liquid are improved by crushing and mixing, the supernatant is extracted twice, waste is avoided, the purity of the muscarinic bacterial liquid can be improved by filtering twice, and the use requirement can be met.
Drawings
FIG. 1 is a flow chart of a process for extracting a mushroom liquid according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example one
Referring to fig. 1, a process for extracting a mushroom liquid includes the following steps:
s1: preparing a fungus;
s2: weighing;
s3: grinding;
s4: heating;
s5: loading into test tube, centrifuging, and mixing the supernatants;
s6: heating and concentrating;
s7: subpackaging and storing.
In this embodiment, in S1, fresh bolete purchased in the last year is prepared, sorted, washed, and then frozen for storage, and when in use, bolete is taken out, thawed in a greenhouse, and then air-dried.
In this example, three portions of bolete were prepared by weighing in S2, and the weights of the bolete were 70 g, 60 g, and 50 g, respectively.
In this example, in S3, bolete is ground, and a sieve is used to perform screening, and a plurality of screens and grinding are performed to obtain bolete powder, and the bolete powder is dissolved in ethanol to obtain a bolete solution.
In this example, in S4, the bolete solution was divided into three portions, and the three portions were heated in a microwave oven for 5 minutes, 10 minutes and 15 minutes, respectively, to obtain triple-cooked, medium-cooked and medium-cooked bolete solutions.
In this example, in S5, three bolete solutions are placed into three test tubes after being completely cooled, the three test tubes are subjected to spin centrifugation, the first supernatant is taken, ethanol is added again to mix, centrifugation is performed again, the second supernatant is taken, and the two supernatants are mixed.
In this embodiment, S6 adds three bolete supernatants into a distillation flask separately, and heats them at 55-65 ℃, so as to achieve heating concentration and complete volatilization of ethanol (to avoid ethanol poisoning of mice during experiments), and three muscarinic bacteria liquids are obtained, and labeled as A, B, C three muscarinic bacteria liquids.
In this example, the dose was recorded and the concentration calculated at S7 was dispensed and then placed into a freezer for use.
In this example, three prepared A, B, C types of muscarinic bacterial liquids were subjected to a mouse experiment:
Figure BDA0003034577440000041
Figure BDA0003034577440000051
therefore, only the semi-immature bolete is processed to extract toxic muscarinic bacteria liquid.
Example two
A process for extracting a mushroom liquid comprises the following steps:
s1: preparing a fungus;
s2: weighing;
s3: grinding;
s4: heating;
s5: loading into test tube, centrifuging, and mixing the supernatants;
s6: heating and concentrating;
s7: subpackaging and storing.
In this embodiment, in S1, fresh chaxingqing purchased in the last year is prepared, sorted, washed, and then frozen for storage, and when in use, the chaxingqing is taken out, thawed in a greenhouse, and then dried.
In this example, weighing was performed in S2 to prepare for the weight of the blue-hand, which was 68 g each.
In this example, the chaenomeles speciosa is ground in S3, and screened with a screen, and a plurality of screens and grinding are performed to obtain chaenomeles speciosa powder, and the chaenomeles speciosa powder is dissolved in ethanol and mixed to obtain a chaenomeles speciosa solution.
In this example, the Okinawa was heated in S4 for 5 minutes in a microwave oven to prepare a half-cooked Okinawa solution.
In this example, after the light blue solution in S5 is completely cooled, the light blue solution is placed in a test tube, the test tube is spun and centrifuged, the first supernatant is taken, ethanol water is added again to mix the first supernatant, the second supernatant is centrifuged again, and the second supernatant is taken and mixed with the first supernatant.
In this embodiment, in S6, the supernatant of radix isatidis is added into a distillation flask and heated at 55-65 ℃ to achieve concentration and complete volatilization of ethanol, so as to obtain a muscarinic bacterial solution.
In this example, the dose was recorded and the concentration calculated at S7 was dispensed and then placed into a freezer for use.
EXAMPLE III
A process for extracting a mushroom liquid comprises the following steps:
s1: preparing a fungus;
s2: weighing;
s3: grinding;
s4: heating;
s5: loading into test tube, centrifuging, and mixing the supernatants;
s6: heating and concentrating;
s7: subpackaging and storing.
In this example, in S1, fresh limacin purchased in the last year is prepared, sorted, washed, and then frozen for storage, and when used, the limacin is taken out, thawed in a greenhouse, and then dried.
In this example, in S2, the weight of the limestone bacteria was 49 g, and the weight of the limestone bacteria was prepared by weighing.
In this example, in S3, the grifola frondosa is ground, and screened with a screen, and a plurality of the screened and ground grifola frondosa powders are extracted, dissolved in 100ml of ethanol, and mixed to obtain a grifola frondosa solution.
In this example, in S4, the solution of the limescale bacteria was heated in a microwave oven for 7 minutes to prepare a half-cooked limescale bacteria solution.
In this example, after the limescale bacteria solution is completely cooled in S5, the limescale bacteria solution is placed in a test tube, the test tube is spun and centrifuged, the first supernatant is taken, ethanol water is added again to mix the first supernatant, the second supernatant is centrifuged again, and the second supernatant is taken and mixed with the first supernatant.
In this embodiment, the supernatant of the limacin is added into a distillation flask and heated at 55-65 ℃ to achieve heating concentration and complete volatilization of ethanol, so as to obtain the muscarinic bacteria liquid.
In this example, the dose was recorded and the concentration calculated at S7 was dispensed and then placed into a freezer for use.
Example four
A process for extracting a mushroom liquid comprises the following steps:
s1: preparing a fungus;
s2: weighing;
s3: grinding;
s4: heating;
s5: loading into test tube, centrifuging, and mixing the supernatants;
s6: heating and concentrating;
s7: subpackaging and storing.
In this embodiment, in S1, fresh pyrocarbon bacteria purchased in the last year are prepared, sorted, washed, and then frozen for storage, and when in use, the pyrocarbon bacteria are taken out, placed in a greenhouse for thawing, and then air-dried.
In this example, pyrocarbon was prepared by weighing in S2, and the weight of the pyrocarbon was 55 g.
In this example, in S3, charcoal was uniformly ground, a screen was used to screen, a plurality of charcoal fungus powders were screened and ground, and 50ml of ethanol was added to dissolve and mix them uniformly to obtain a charcoal fungus solution.
In this example, in S4, the pyrocarbon bacterium solution was heated in a microwave oven for 6 minutes to prepare a semi-crude pyrocarbon bacterium solution.
In this embodiment, after the pyrocarbon-containing bacterial solution is completely cooled in S5, the tube is placed in a test tube, the test tube is spun and centrifuged, the first supernatant is taken, ethanol water is added again to mix the first supernatant, the second supernatant is centrifuged again, the second supernatant is taken, and the two supernatants are mixed.
In this embodiment, the supernatant of the pyrocarbon bacterium is added into a distillation flask and heated at 57-60 ℃ to achieve heating concentration and complete volatilization of ethanol, thereby obtaining the muscarinic bacteria liquid.
In this example, the dose was recorded and the concentration calculated at S7 was dispensed and then placed into a freezer for use.
EXAMPLE five
A process for extracting a mushroom liquid comprises the following steps:
s1: preparing a fungus;
s2: weighing;
s3: grinding;
s4: heating;
s5: loading into test tube, centrifuging, and mixing the supernatants;
s6: heating and concentrating;
s7: subpackaging and storing.
In this example, in S1, fresh termitomyces albuminosus purchased in the last year is prepared, sorted, washed, frozen and stored, and when in use, the termitomyces albuminosus is taken out, thawed in a greenhouse, and then dried.
In this example, termitomyces albuminosus was weighed in S2, and the weight of termitomyces albuminosus was 50 g.
In this example, in S3, the charcoal was uniformly ground, and the powder was screened using a screen, and a plurality of screens and grindings were performed to obtain a termitomyces albuminosus powder, which was dissolved in 40ml of ethanol water and mixed uniformly to obtain a termitomyces albuminosus solution.
In this example, in S4, the termitomyces albuminosus solution was heated in a microwave oven for 7 minutes to prepare a half-cooked termitomyces albuminosus solution.
In this example, in S5, after the termitomyces albuminosus solution is completely cooled, the termitomyces albuminosus solution is placed in a test tube, the test tube is rotated and centrifuged, the first supernatant is taken, ethanol water is added again to mix the first supernatant, the second supernatant is taken again, and the supernatants taken twice are mixed.
In this embodiment, the supernatant of Collybia albuminosa is added into a distillation flask and heated at 55-65 ℃ to achieve heating concentration and complete volatilization of ethanol, so as to obtain the muscarinic bacteria liquid.
In this example, the dose was recorded and the concentration calculated at S7 was dispensed and then placed into a freezer for use.
EXAMPLE six
S1: preparing a fungus;
s2: weighing;
s3: grinding;
s4: heating;
s5: loading into test tube, centrifuging, and mixing the supernatants;
s6: heating and concentrating;
s7: subpackaging and storing.
In this embodiment, in S1, a fresh dry powder of coprinus atramentarius purchased in the last year is prepared, sorted, washed, and then frozen for storage, and when in use, the dry powder of coprinus atramentarius is taken out, placed in a greenhouse for thawing, and then dried.
In this example, in S2, a dry coprinus fungus powder was prepared, and the weight of the dry coprinus fungus powder was 250 mg.
In this example, in S3, charcoal is uniformly ground, and screened with a screen, and a plurality of screens and grinds are performed to obtain dry powder of coprinus atramentarius, and 5ml of ethanol water and ethanol are added to dissolve and mix uniformly, so as to obtain a dry powder solution of coprinus atramentarius.
In this example, in S4, the dry powder solution of coprinus atramentarius was heated in a microwave oven for 6 minutes to prepare a semi-immature dry powder solution of coprinus atramentarius.
In this embodiment, after the coprinus fungus dry powder solution is completely cooled in S5, the coprinus fungus dry powder solution is placed in a test tube, the test tube is rotated and centrifuged, the first supernatant is taken, ethanol water and ethanol are added again to mix, centrifugation is performed again, the second supernatant is taken, and the supernatants obtained twice are mixed.
In this embodiment, S6 adds the supernatant of the dry powder of coprinus atramentarius into a distillation flask and heats the supernatant at 57-62 ℃ to achieve heating concentration and complete volatilization of ethanol to achieve heating concentration, thereby obtaining the muscarinic bacteria liquid.
In this example, the dose was recorded and the concentration calculated at S7 was dispensed and then placed into a freezer for use.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (8)

1. A process for extracting a mushroom liquid is characterized by comprising the following steps:
s1: preparing a fungus;
s2: weighing;
s3: grinding;
s4: heating;
s5: loading into test tube, centrifuging, and mixing the supernatants;
s6: heating and concentrating;
s7: subpackaging and storing.
2. The extraction process of a poisonous mushroom liquid as claimed in claim 1, wherein the fresh bolete purchased last year is prepared in S1, sorted, washed, and then frozen for storage, and when in use, the bolete is taken out, placed in a greenhouse for thawing, and then dried.
3. The process of claim 1, wherein the step of S2 comprises weighing three different weight portions of Boletus edulis, wherein the weight portions of Boletus edulis are 70 g, 60 g and 50 g.
4. The extraction process of claim 1, wherein in step S3, the Boletus edulis is ground, screened by using a screen, and a plurality of the screened and ground Boletus edulis powders are dissolved in ethanol to obtain a Boletus edulis solution.
5. The extraction process of a poisonous mushroom liquid as claimed in claim 1, wherein the bolete solution is divided into three parts in S4, and the three parts are heated by microwave oven for 5 minutes, 10 minutes and 15 minutes to obtain triple-cooked, quintuple-cooked and complete-cooked bolete solutions.
6. The process of claim 1, wherein the step of S5 comprises the steps of cooling three bolete solutions completely, loading the bolete solutions into three test tubes, centrifuging the three test tubes, collecting the first supernatant, mixing the first supernatant with ethanol again, centrifuging the second supernatant again, collecting the second supernatant, mixing the two supernatants, and filtering the mixture again with a filter membrane.
7. The extraction process of a poisonous mushroom liquid as claimed in claim 1, wherein the S6 is obtained by heating three bolete supernatants separately in a distillation flask at 55-65 deg.C to achieve concentration and complete volatilization of ethanol.
8. The process of claim 1, wherein the S7 is recorded and the concentration is calculated and dispensed, and then placed in a freezing chamber for further use.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114903923A (en) * 2022-05-26 2022-08-16 桂红珍 Application of boletus extract in preparation of medicines for treating depression

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101744209A (en) * 2009-12-14 2010-06-23 楚雄宏桂绿色食品有限公司 Porcini essential oil, essential oil seasoning and preparing method thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101744209A (en) * 2009-12-14 2010-06-23 楚雄宏桂绿色食品有限公司 Porcini essential oil, essential oil seasoning and preparing method thereof

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* Cited by examiner, † Cited by third party
Title
无: "牛肝菌为什么要煮15分钟", 《HTTPS://WWW.LDSHIJIE.COM/C/31537/》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114903923A (en) * 2022-05-26 2022-08-16 桂红珍 Application of boletus extract in preparation of medicines for treating depression
CN114903923B (en) * 2022-05-26 2023-08-25 桂红珍 Application of boletus extract in preparation of medicines for treating depression

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