TWI523603B - Method and equipment for solidification and fermentation of solid fermentation - Google Patents

Description

Solid-state fermenting fruit body cultivation and fusion method and equipment

The invention provides that the burdock mushroom fruit body can prolong the breeding time course, so as to be able to transfer higher triterpenoid value.

Regarding the breeding of burdock mushroom, in addition to the discovery in nature in the early stage, artificial cultivation methods have recently been adopted due to the demand of quantity, and the commonly used medium is mainly composed of wood chips, starch, sugar and gypsum powder to form a cultured substrate and For bottling culture, during the breeding process, the mixing of bacteria and the control of excessive temperature and the adjustment of illuminance are noted. The utensils used are provided with breathing, so that the air in the bottle can communicate with the outside, but The kinetic energy of communication is the essential respiration from the development of the fruiting body. The energy of the airflow is small, so the air exchange rate is not high, and the growth process is free from growth to solid growth. The mixed medium has a fast growth. The effect of fermentation is to be harvested before the medium is most effective. Otherwise, due to the rapid acidification of the medium, the fruiting bodies that have not been fully matured after acidification are maimed.

The invention provides an extended time course of the fruiting body, wherein during the satisfied period, the nutrient of the medium can be melted for a long period of time to form a triterpenoid component, so that the harvested burdock mushroom has a higher value of three mites. It is mainly cultivated in a low-temperature and low-oxygen environment. During the process, the air inside and outside the container is exchanged, and the culture medium is blended into a solid and low-acidity. Finally, the operation is carried out, and the nutrient of the medium is vigorously extracted according to the growth energy demand. So that the completed sub-entities accumulate more high-quality triterpenes for their main purpose.

Still another object of the present invention is to provide a forced airflow exchange arrangement that can utilize a conduit system via pressure The force modulation forces the exchange of airflow inside and outside the bottle.

A third object of the present invention is to use an artificial light beam to trigger its growth or excitability state in addition to temperature operation in a culture environment.

1‧‧‧Reflecting container

11‧‧‧ stopper

110‧‧‧through hole

12‧‧‧ breathable cotton room

13‧‧‧Airflow line

130‧‧‧Air compressor

2‧‧‧ medium

3‧‧‧ cultivation work

301‧‧‧Cell preparation work

302‧‧‧Study operation

31‧‧‧Low temperature maintenance operation

32‧‧‧ gas exchange operation

321‧‧‧ Temperature difference handover operation

322‧‧‧Pressure push operation

4‧‧‧Exciting work

41‧‧‧heating operation

42‧‧‧beam triggering operation

421‧‧‧ natural beam

422‧‧‧Artificial beam

423‧‧‧Artificial light source

5‧‧‧ Harvesting operations

50‧‧‧Mycelium

500‧‧‧Sub-entities

Figure 1 is a schematic diagram of the implementation of a cultured fruit container.

Figure 2 is a flow chart of the application method of the present invention.

Figure 3 is a time chart of the forced gas exchange of the present invention.

Figure 4 is a system diagram of the air compressor and the refractive container provided by the pressure pushing operation of the present invention.

Figure 5 is a schematic diagram of natural daylight waves.

The invention provides an cultivation method capable of prolonging the growth period of the fruiting body and having low temperature hibernation, and finally carrying out the process of stimulating and low temperature cultivation, which can fully blend the nutrient of the culture medium, so that the triterpenoids after the growth are higher. And the final excitement work, which will accumulate higher triterpenes with high energy, using low-acidity medium for breeding, and forcing manipulation of gas exchange inside and outside the container during the growth process. Solid particles can be fully integrated.

Regarding the cultivation method of artificially cultivating the burdock mushroom, a refracting container 1 (as shown in Fig. 1) is arranged, and the medium 2 is placed on the bottom, and the surface of the medium 2 is used for the cultivation of the mycelium 50, and the mycelium 50 is grown. After the fruit body 500 is formed, a stopper 11 is disposed in the mouth of the refractive container 1 in the process, and the stopper 11 is passed through the through hole 110 to prevent external sudden air or bacteria from entering the refractive container 1 through the filtration of the air permeable cotton chamber 12. Internally, during the growth process, the mycelium 50 or the fruit body 500 exchanges the air inside the container with the outside world. Its exchange operation pushes the air according to its own combined energy, so that the inside and outside air are exchanged, but the rate is not good. Therefore, the fruit body 500 inside the refractive container 1 cannot obtain a comprehensive combination, so that the fruit body 500 or the mycelium 50 inside the refractive container 1 can be comprehensively compounded.

The air inside and outside the forced refraction vessel 1 of the present invention can be fully exchanged, and the culture used Nutrient 2 can be made with a low acidity component, and the formulated formula can be one of the following:

1. Burdock wood chips 77%. Bran 18%. Corn flour 3%. Sucrose 1%. Gypsum powder 1%

2. Bagasse 50%. Burdock wood shavings 48%. Soybean flour 1%. Gypsum powder 1%

3. Cottonseed hull 44%. Burdock wood shavings 44%. Bran 5%. Corn flour 5%. Sucrose 1%. Gypsum powder 1%

4. Corn cob 45%. Burdock wood 45%. Bran 8%. Soybean powder 1%. Gypsum powder 1%

5. Wheat 55%. Corn 10%. Coix seed 9%. Peptide 3%. Peptone 5%. Peanut powder 16%. Sucrose 1%. Gypsum powder 1%

6. Germinated wheat 45%. Sorghum 45%. Peptide 4%. Peptone 6%.

7. Germinated wheat 41%. Corn 42%. Black beans 5%. Peptide 3%. Peptone 4%. Bran 3%. Sucrose 1%. Peanut powder 1%.

8. Wheat 42%. Oats 31%. Coix seed 15%. Peptide 4%. Peptone 6%. Soybean powder 2% or more 8 kinds.

In addition, under the operation of inhibiting acidity, the same agent for adding acid can be added, such as gypsum, but it is not suitable to use the compound product. The above eight kinds of formulas are in the form of silt, and the components thereof can also be used as granules of hydrazine. For the solid matter, such as wheat or coix seed, it can delay the rate of fermentation. Any natural ingredient that can delay the fermentation rate of the medium is the medium of the object, which can prolong the fermentation rate and provide the same The growth process of the fruiting body can be extended, and the nutrient of the fully blended medium can be converted into triterpenes in an extended time course, so that the triterpenoids of the harvesting fruit body are more valuable.

Regarding the method for carrying out the present invention, (see FIG. 2), a medium having a low acidity is prepared in advance, and a medium preparation operation 301 is prepared for the refracting container to perform a germination operation 302 to fertilize the fruit body, which controls growth. The time course is about 90 days, and the process is carried out by low temperature and low oxygen. When the fruiting body is developed, the cultivation operation is carried out. 3, the cultivation operation 3 performs low temperature maintenance operation 31 and gas exchange in addition to the low oxygen environment. Operation 32.

The gas exchange operation 32 can be operated in two ways, wherein the air inside the refraction vessel 1 can be fully exchanged with the outside by means of the temperature difference delivery operation 321, or the pressure push operation 322 can be performed mechanically. Clearly allow the air inside and outside the container to reach full communication.

The time course of the cultivation operation 3 is maintained for about 30 days, and the operation 4 is performed. The operation of the lengthening operation 4 can be performed by the placement of the temperature increasing operation 41 or the intervention of the light beam triggering operation 42 but for the auxiliary beam triggering operation 42. It can be achieved by using the natural beam 421 and the artificial beam 422. In operation 4, the harvesting operation can be carried out after the catalytic excitation is maintained for 30 days.

The temperature difference handoff operation 321 of the above-mentioned cultivation operation 3 has an operating frequency as shown in FIG. 3, which can be modulated between 15 and 25 degrees (in accordance with the practice of the present invention, the indoor temperature is maintained at 25 degrees) The curve of the test) is cyclically alternated for 5 to 10 days. At room temperature of 25 degrees, the inside of the container also reaches 25 degrees with the effect of heat balance, and then the indoor air is cooled and lowered. In the process of 15 degrees, since the inside of the container is closed, its temperature drop rate is slow, so the air with higher external density will enter the inside of the bottle and exchange with it, and the residual carbon dioxide will be pushed during the exchange process. Oxygen is added. In the same process, when the external air is raised, the internal air is closed and the heating rate is slow. Therefore, the dense air can be exchanged by the physical phenomenon of the temperature difference. The path of the exchange is as shown in Fig. 1. The through hole 110 is shown in and out. The effect of the forced exchange is that the air inside the container can be fully supplied to provide the comprehensive requirement of the internal fruit body, so the exchange of air is cultivated by the oyster mushroom, especially in the closed bottle. In the medium environment, it is a mandatory and conditional mandatory operation that enables the development of the fruiting body to be more complete.

Please refer to FIG. 4 again, wherein the operation method of the forced pressure pushing operation 322 (please be shown in FIG. 2) can be used to guide the majority of the refractive containers via the distributed air flow line 13 by the air compressor 130. 1. The gas flow line 13 is electrically connected to the inside of the refractive container 1 via the through hole 110 provided in the stopper 11 of the refractive container 1, and the stopper 11 itself is refracted in addition to the closed shape or the other way of opening the guide hole. The internal pressure of the container 1 changes the air to enter and exit from the portion of the stopper 11, wherein the air compressor 130 generates a pressurized air pressure, and the temperature can be 15 to 25 degrees as described above (the growth temperature is preferably 25 degrees). Between, by the pressure of the air compressor 130, it can input external air into the external fresh air via the path of the differential conduction inside the refractive container 1, and when the air compressor 130 generates pressure into the internal input of the refractive container 1, it The gas remaining inside is internally squeezed out, and a forced manner of exchanging the air inside the refractive container 1 with the outside is obtained, and the duty cycle of the air compressor 130 can be simulated as shown in FIG.

In addition, in the operation mode of the exciting operation 4, in addition to the manner of introducing the external natural light beam 421, the beam triggering operation 42 can further utilize the mode of the artificial light beam 422, and the light wave generated by the artificial light beam 422 is as shown in FIG. It shows that it can simulate the natural light, but in the wavelength range of about 550~780, it has obvious catalytic effect on plant growth, and an artificial light beam 422 is used to refract. The light beam that occurs in the space of the container 1, which will pass through the refractive volume The refraction of the device 1 enters the interior of the refractive container 1, and the illumination frequency of the artificial light source 423 can mimic the time course of nature day and night, and the waveform of the artificial light source 423 can be modulated by the help of other levels such as layers. The light wave, such as the morning sun is warm white or the warm white, the wave behavior of the light wave can be changed, and even the color temperature of the evening can be modulated by the logic circuit to change the artificial light source 423. The light beam can simulate the undulating wave of the sun light in the morning faint. According to this, the space for the growth of the oyster mushroom is in the dark area, and the artificial light source 423 can be modulated by other modulation methods to change the light wave pattern in the dark. With the intervention of the artificial light beam 422, it can be closedly cultured in a closed space such as a basement, and with the aid of the program, it can control the operation of the air compressor 130 to achieve fully automated cultivation operation, and The temperature rise operation of the demanding operation 4 is 41. It is determined by the temperature conditions of the geographical environment. If it is in the cold zone, the internal temperature can be increased by artificial electric heating. Li ambient temperature met, then it can be directly brought into the space natural hot temperature of the culture, growth of fruiting bodies is triggered.

The invention utilizes a low-acid medium and a low-temperature and low-oxygen culture method, so that the child sports period can be prolonged, and then the excitatory operation is carried out afterwards, and in the process of prolonging and agitation of the mycelium and the child sports period, The nutrient of the culture medium is fully absorbed and transformed into high-priced triterpenoids, and the fermentation rate of the medium is slow, which can support the extension of the growth period of the fruit body of the burdock mushroom, and the environment of low temperature and low oxygen during the growth process. In addition, the gas exchange inside and outside the compulsory exchange container allows the air inside the container to be fully filled to meet the compounding needs of the oyster mushroom entity. It is a brand new fruiting body cultivation method. It is fortunate to give a clear understanding and to give invention patents as soon as possible.

3‧‧‧ cultivation work

301‧‧‧Cell preparation work

302‧‧‧Study operation

31‧‧‧Low temperature maintenance operation

32‧‧‧ gas exchange operation

321‧‧‧ Temperature difference handover operation

322‧‧‧Pressure push operation

4‧‧‧Exciting work

41‧‧‧heating operation

42‧‧‧beam triggering operation

421‧‧‧ natural beam

422‧‧‧Artificial beam

5‧‧‧ Harvesting operations

Claims (10)

A solid-state fermenting fruiting body cultivation and infusion method, in particular, providing a bovine mushroom fruiting body to prolong the breeding time course, so as to be able to transfer higher triterpenoid value, including: preparing a medium for preparing a low acid value medium a planting operation, placing the above low acid value medium in a container, and then performing the mycelium cultivation operation in a low temperature and low oxygen environment space, and the process of the cultivation operation simultaneously forces the gas exchange operation; The long operation is the temperature-increasing operation mode that can be involved in the beam triggering, and the temperature difference hand-over operation is alternately performed with a periodicity of 5 to 10 days in a certain temperature range. The solid-state fermenting fruit body cultivation and fusion method according to claim 1, wherein the low-acid component of the medium may be one of the following groups: A. Burdock wood chips 77%, bran 18%, corn flour 3%. 1% sucrose, 1% gypsum powder; B. 50% sugarcane bagasse, 48% burdock wood, 1% soybean powder, 1% gypsum powder; C. 44% cotton seed hull, 44% burdock wood, 5% bran Corn flour 5%, sucrose 1%, gypsum powder 1%; D. corn cob 45%, burdock wood 45%, bran 8%, soybean powder 1%, gypsum powder 1%; E. wheat 55%, corn 10%, 9% of coix seed, 3% of peptide, 5% of peptone, 16% of peanut powder, 1% of sucrose, 1% of gypsum powder; F. 45% of germinated wheat, 45% of sorghum, 4% of peptide, and 6% of peptone; G. Germinated wheat 41%, corn 42%, black beans 5%, peptide 3%, peptone 4%, bran 3%, sucrose 1%, peanut powder 1%; H. wheat 42%, oats 31%, coix seed 15 %, peptide 4%, peptone 6%, soy flour 2%. The solid-state fermenting fruit body cultivation and fusion method according to claim 1, wherein the gas exchange operation of the cultivation operation is to pressurize the air in the external cultivation space of the container by using a pressure pushing operation, and then conduct the catheter through the conduit. The interior of the container is pushed up. The solid-state fermenting fruit body cultivation and fusion method according to claim 1, wherein the beam triggering operation is a natural beam or an artificial beam. The solid-state fermenting fruit body cultivation and fusion method according to claim 4, wherein the artificial beam system can generate light waves for simulating natural light, and the illumination frequency can be modulated by the time and day of nature day and night. Light waves that are needed for the morning faint to facilitate cultivation in a closed environment, And, through the program's auxiliary and air compressor, fully automated cultivation operation. The method of claim 1, wherein the time course of the cultivation operation is 30 days. The solid-state fermenting fruit body cultivation and fusion method according to claim 1, wherein the time course of the excitatory operation is 30 days. The utility model relates to a device for cultivating and transferring a solid-state fermenting fruit body, comprising: a plurality of refractive containers, which are provided with a cork, the cork is opened by the through hole, and the through hole of the cork is provided with a gas permeable cotton chamber to avoid external sudden air. Or bacteria enter the interior of the refractor vessel; a low-temperature, low-oxygen space with refrigeration equipment is provided for the cultivation operation; a facility required for a gas exchange operation. The apparatus for cultivating and transferring a solid-state fermenting fruit body according to claim 8 wherein the gas exchange operation facility is an air compressor disposed in the space and is electrically connected to the inside of the refractive container. The apparatus for cultivating a fusion of the solid-state fermenting fruit body according to claim 8 of the patent application, further comprising an artificial light beam.