TWI488632B - Wound care composition and wound care spray - Google Patents

Description

Wound care composition and wound care spray

The present invention relates to a material for wound treatment, and more particularly to a wound care composition and a wound care spray.

In general, when a wound appears on the skin and its integrity is destroyed, it will not function properly. If the wound is not handled properly, it may be infected by the pathogen and cause inflammation. Therefore, treating the wound in a proper and appropriate manner is important to avoid wound infection and promote wound healing.

Previously, many products have been developed that can be applied to wound care, and with the in-depth study of medical materials in recent years, how to further improve the convenience and efficacy of wound care materials in use has become a medical material. One of the main topics in development.

The role of wound dressings in curing or repairing skin damage is very important, but most of the products currently on the market are mostly film-like or powdery, which often causes inconvenience to the use of specific parts, and in terms of promoting and antibacterial effects. Not necessarily ideal. In addition, if the wound on the skin is exposed to the sun or other radiation (such as purple Damage such as outside lines may cause slower healing or sunburn problems in newborn skin, and may also have an effect on healing speed and aesthetics after healing. Therefore, in the development of wound care materials, in addition to the convenience of use, we must also consider how to provide better protection of the skin.

Based on the above, it is necessary to further develop a wound care material which is superior in terms of ease of use, promotion, antibacterial effect, and protectiveness.

The present invention provides a wound care composition and a wound care spray which can promote wound healing, avoid infection, enhance skin protection, and facilitate care.

The wound care composition provided by the present invention includes water-soluble chitosan, collagen, and alcohol compound; wherein, based on 100% of the total weight of the above composition The content of the above collagen is 0.05% to 1%.

In an embodiment of the invention, the water-soluble chitosan has a molecular weight in the range of 100 kDa to 150 kDa.

In an embodiment of the invention, the pH of the wound care composition is in the range of 6.0 to 7.0.

In one embodiment of the present invention, the water-soluble chitosan is contained in an amount of from 0.125% to 4% based on 100% of the total weight of the wound care composition.

In an embodiment of the invention, the alcohol compound is selected from the group consisting of At least one of the group consisting of alcohol, isopropanol, propanol, and butanol.

In an embodiment of the invention, the water-soluble chitosan has a degree of deacetylation of 80% or more.

In an embodiment of the present invention, the content of the alcohol compound is 15% to 50% based on 100% by total of the total weight of the wound care composition.

In an embodiment of the invention, the content ratio of the water-soluble chitosan to the collagen is from 2:1 to 10:1.

The wound care spray provided by the present invention comprises the wound care composition as described above and a volatile carrier, and the above volatile carrier comprises carbon dioxide gas. In an embodiment of the invention, the ratio of the amount of the carbon dioxide gas introduced to the volume of the composition solution is 1:1 to 1:2.

Based on the above, the wound care composition and the spray provided by the present invention can promote wound healing, quickly restore the wound, accelerate cell proliferation, improve blood circulation around the wound, and have an excellent effect on antibacterial and hemostasis, and can prevent In addition, the wound care composition and the spray provided by the present invention have an absorption effect on ultraviolet rays, thereby reducing the possibility of sunburn, and are also convenient in use, and are used for treating small wounds in daily life, Or it is quite effective to deal with burns and burns on the shallow surface of the skin.

The above described features and advantages of the invention will be apparent from the following description.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a flow chart showing a method of preparing a wound care composition and a spray according to an embodiment of the present invention.

Figure 2 shows the results of an antibacterial test of a composite formulation containing chitosan components with different degrees of deacetylation.

Figures 3(a) through 3(d) show the UV absorption test results for different composite formulations.

4 is a test result of a wound care spray in an animal experiment in accordance with an embodiment of the present invention.

The composition for wound care provided by the present invention includes water-soluble chitosan, collagen, and an alcohol compound; wherein the content of the collagen is 0.05% to 1% based on 100% by weight of the total composition.

The above water-soluble chitosan is, for example, a chitosan having a solubility in water of about 95% or more. The water-soluble chitosan is obtained, for example, by hydrolysis treatment (deacetylation) of chitin. The source or preparation manner of the water-soluble chitosan used in the wound care composition of the present invention is not particularly limited, and those skilled in the art can prepare it by itself or obtain a desired product from a commercial product. Water soluble chitosan material.

The above water-soluble chitosan can bring about a bacteriostatic effect, and the amount of addition can be adjusted according to the use. For example, the composition for wound treatment of the present invention is further prepared When spraying, the amount of chitosan added can be adjusted by considering the size of the nozzle and the pressure of the filling tank, so as to avoid the situation that the spray is blocked and the like is affected. Specifically, the content of the water-soluble chitosan is, for example, 0.125% to 4%, more preferably 0.25% to 2%, particularly preferably 0.5%, based on 100% of the total weight of the wound care composition. ~1.5%.

Further, the molecular weight of the water-soluble chitosan is preferably in the range of 100 kDa to 150 kDa, more preferably in the range of 115 kDa to 135 kDa, and particularly preferably in the range of 120 kDa to 130 kDa. By setting the molecular weight distribution of the water-soluble chitosan in the composition, the viscosity of the composition solution or the like can be appropriately adjusted according to the use.

Further, the degree of deacetylation of the above water-soluble chitosan is, for example, 80% or more, more preferably 85% to 90%, particularly preferably 90% to 95%. By using a water-soluble chitosan having a degree of deacetylation of 80% or more, a more desirable bacteriostatic effect is obtained.

The source of the above collagen is not particularly limited, and those skilled in the art can select it at their own discretion. For example, collagen extracted from pig skin, cowhide, fish skin or fish bone, fish scale, fin, or the like can be used, but is not limited thereto. The addition of collagen helps promote cell proliferation and is more conducive to wound repair.

In terms of cost and effect of addition, the content of the collagen is 0.05% to 1%, and more preferably 0.075% to 0.75%, based on 100% of the total weight of the wound care composition. 0.1%~0.4%. In general, the content of the above water-soluble chitosan and the above collagen is preferably 2:1 to 10:1, more preferably 3:1 to 8:1, and particularly preferably 4:1 to 6:1.

It is worth noting that by the composition of the wound care of the present invention When added with water-soluble chitosan and collagen, it can also absorb light for ultraviolet light in a specific wavelength range.

Generally, ultraviolet rays can be classified into UV-A (about 320 nm to 400 nm), UV-B (about 290 nm to 320 nm), and UV-C (about 100 nm to 290 nm) depending on the wavelength, wherein UV-B is for the human body. The most harmful, it is a risk factor for skin cancer, sunburn, peeling and so on. The present inventors have found through experiments that the wound care composition according to the embodiment of the present invention has an absorption effect on ultraviolet rays having a wavelength of about 240 nm to 320 nm, that is, it can absorb UV-B and some UV-C, This reduces the likelihood of sunburn and provides better protection against the skin (wound) surface.

Therefore, the wound care composition of the present invention is particularly suitable for wound treatment of burnt sunburn (for example, burn of 1-2 grade) in the superficial layer of the skin, in addition to avoiding the increase of heat energy on the burnt wound of the burnt sun. The increase in the rate of skin tissue necrosis can also prevent the formation of sunburn.

Further, the pH of the wound care composition is preferably in the range of 6.0 to 7.0 from the viewpoint of being more suitable for application to a body surface wound.

The content of the above alcohol compound is preferably from 15% to 50%, more preferably from 25% to 45%, particularly preferably from 35% to 40%, based on 100% of the total weight of the wound care composition. By using a volatile alcohol compound as a solvent, when the wound care composition is applied to a wound, the temperature of the skin surface can be lowered, thereby bringing a little refreshing feeling to the skin burnt by the skin surface. The heat pain caused by it is especially soothing.

Specifically, the above alcohol compound as a solvent is preferably selected from the group consisting of At least one of the group consisting of alcohol, isopropyl alcohol, propanol and butanol is not limited thereto, and those having ordinary knowledge in the art can select an appropriate one according to factors such as cost or added effect. Solvent composition. Among them, ethanol is preferably used from the viewpoint of further enhancing the bactericidal effect.

As described above, the wound care composition provided by the present invention has the effects of antibacterial, hemostasis, and rapid cell proliferation, and can not only reduce wound infection, but also has an absorption effect on ultraviolet rays, thereby reducing the possibility of sunburn, and thus application. It can promote rapid recovery of wounds and reduce the production of scar tissue.

In another aspect, the wound care spray of the present invention comprises the above wound care composition and a volatile carrier, and the volatile carrier comprises carbon dioxide gas.

Specifically, the carbon dioxide gas as a volatile carrier can be mixed with the wound care composition by filling the can to prepare a wound care spray. In more detail, a wound care dressing in the form of a spray can be made by passing a predetermined amount of carbon dioxide into a volume of wound care composition solution at a specific pressure. Further, the pressure is not particularly limited as long as the pressure of carbon dioxide can be introduced into the filling tank, and the ratio of the amount of carbon dioxide to the volume of the composition solution is, for example, 1:1 to 1. : 2, but it is not limited thereto, and those skilled in the art can adjust it according to requirements.

According to the Bohr effect, a large amount of hydrogen ions (low pH environment) or carbon dioxide will reduce the affinity of hemoglobin to oxygen, which causes hemoglobin to release oxygen. More specifically, when carbon dioxide reacts with water, Hydrogen ions are released, causing the pH to decrease, resulting in a decrease in the oxygenation index of hemoglobin hemoglobin. When the oxygenation rate decreases, the oxygen bound to the hemoglobin is released, causing the partial pressure of oxygen to rise. When the oxygen in the blood rises, the cells around the blood vessels can more easily use oxygen to promote cell metabolism and improve blood circulation. Therefore, the use of carbon dioxide gas as a volatile carrier can further promote cell metabolism, improve blood circulation, and thereby shorten wound healing time.

Further, the volatile carrier may further include air or nitrogen as a carrier without affecting the effect of the spray, but is not limited thereto. Hereinafter, a method applicable to the preparation of the wound care composition and the spray of the present invention will be further described with reference to FIG.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a schematic flow chart showing a method for preparing a wound care composition and a spray according to an embodiment of the present invention. As shown in Fig. 1, first, a water-soluble chitosan solution and a collagen solution can be separately formulated.

Next, the water-soluble chitosan solution and the collagen solution obtained above are mixed and stirred using an alcohol compound as a solvent to obtain a composite formulation (that is, a wound care composition). The above composite formulation can be applied directly to wound care, or indirectly to wound treatment by attachment to a suitable dressing such as gauze, cotton pad, or the like.

Thereafter, when a wound care material in the form of a spray is to be prepared, a gas filling can be further performed, and a gas molecule (including carbon dioxide) as a carrier is introduced into the composite formulation to prepare a spray.

Further, the wound care spray of the present invention is gradually solidified after being sprayed on a wound in a liquid state, and forms a film on the wound and the peripheral skin. The formed film also has a protective effect on the skin, and since it contains water-soluble chitosan and collagen, it has an absorption effect on ultraviolet rays, thereby reducing the possibility of sunburn.

As described above, the wound care spray of the present invention is obtained by filling a can with a gas containing carbon dioxide, which can improve blood circulation around the wound during application and effectively promote wound healing. Further, in practical use, the wound care composition and the spray of the present invention are also sufficiently convenient.

Hereinafter, several experimental examples will be presented to further elaborate the wound care composition and spray of the present invention. It should be noted that the data results of the following experimental examples are merely illustrative of possible preparation methods or compositions of wound care compositions and sprays of the embodiments of the present invention, and test results after various treatments or experiments. It is not intended to limit the scope of the invention.

[Experimental Example 1] Antibacterial ability test (change in chitosan deacetylation degree)

In order to test the degree of deacetylation of chitosan, the degree of antibacterial effect was affected, and the antibacterial ability test was performed. In this experiment, the sample was continuously diluted, and the minimum inhibitory concentration (MIC) of the sample was confirmed by a paper disk method. In this experiment, the antibacterial ability test was performed on Staphylococcus aureus.

Experimental procedure: 1. Preparation of samples: Chitosan (manufactured by BIOCHINA Co., Ltd.) was treated according to each group of treatment conditions in Table 1 below, and each group of refined chitosan (de-acetylation degree 52.44 to 90.62, Water soluble) 5g added 500mL electrolyzed water, Each was formulated into a 1% aqueous solution of chitosan. 1 g of fish scale collagen peptide (protein) was taken, and 500 mL of electrolyzed water was added to prepare a 0.2% fish scale collagen peptide (protein) aqueous solution. The aqueous solution of the fish scale collagen peptide (protein) was slowly added to each group of chitosan aqueous solution to be completely mixed during high-speed stirring, and samples 1 to 6 in Table 1 below were obtained.

2. Antibacterial detection: After diluting each of the solutions of samples 1 to 6 by electrolyzed water for 2, 4, 8, 16, and 32 times, the solution of sample 1 to 6 and each group of different diluted solutions are passed through the ring. The minimum inhibitory concentration (MIC) was confirmed by paper ingot method, and the experimental results are shown in Table 1 below.

3. Using this method, the qualitative antibacterial state of the chitosan concentration in the solution is 1%, 0.5%, 0.25%, 0.125%, 0.0625% and 0.03125%, respectively, when the ring-shaped paper ingot is produced. When the fungus ring is determined, the solution has an antibacterial ability, and when the antibacterial concentration range of the sample solution in the experiment can reach 0.03125%, the sample solution is judged to be an effective antibacterial substance, and the sample can be reused for subsequent quantitative antibacterial experiments.

It can be seen from the experimental results in Table 1 that the antibacterial range of Samples 1 to 3 increases with the increase of the degree of deacetylation, but when the degree of deacetylation of the sample reaches about 80% or more (ie, samples 3 to 6), the antibacterial effects were similar (the antibacterial effect was obtained after 32 times dilution) and would not increase.

2 is an experimental result of a ring-shaped paper ingot method of a solution of Sample 6 and a solution diluted by different multiples. It can be seen from Fig. 2 that the solution of the sample 6 can produce an antibacterial ring around the annular paper ingots, whether it is a stock solution or diluted 2 to 32 times, so the sample solution does have antibacterial ability.

As can be seen from the experimental examples, by using a water-soluble chitosan having a degree of deacetylation of 80% or more, a more desirable bacteriostatic effect is obtained.

[Experimental Example 2] Antibacterial ability test (variation of chitosan content)

The content of chitosan will affect the solution state of the composition (such as viscosity, etc.), and when it is desired to make a wound care material in the form of a spray, it is also necessary to consider whether the addition amount of chitosan does not cause the nozzle to block. At the same time, it has sufficient antibacterial ability.

In this experiment, the effect of the change in chitosan content in the sample on the antibacterial ability was mainly tested according to the method of JIS L1902.

Experimental procedure: Refer to the JIS L1902 standard method and its experimental procedures.

1.) In the following experimental results (Table 2), 2.0 E+02 means 200; 1.3 E+04 means 13000, and so on.

2.) Calculation instructions: bacteriostatic activity value = (logMb-logMa) - (logMc'-logMo) bactericidal value = logMa-logMc' (Ma = control group initial bacterial concentration, Mb = control group after 18 hours of culture) The concentration of the bacterial solution, Mo = initial bacterial concentration in the experimental group, and Mc = the concentration of the bacterial liquid after the experimental group was cultured for 18 hours.

3.) When the bacteriostatic activity value is 2.0 or more, it indicates that the sample has an antibacterial effect; When the sterilization value is 0 or more, it indicates that the sample has a bacteriostatic effect.

Sample A: 0.5% chitosan/collagen complex

(1L mixture contains 500mL of 95% alcohol and 500mL of aqueous solution, wherein the chitosan solid content is 5g, collagen is 1g, and the following sample components are deduced by analogy. (The ratio of chitosan to collagen is maintained at 5/1. ))

Sample B: 1% chitosan/collagen complex

Sample C: 2% chitosan/collagen complex

Sample D: 4% chitosan/collagen complex

The experimental results are shown in Table 2 below.

From the experimental results in Table 2, it is known that samples containing higher concentrations of chitosan (such as samples C and D) have obvious antibacterial effects against Staphylococcus aureus, but samples containing higher concentrations of chitosan tend to block the spray cans. Nozzles, while samples containing lower concentrations of chitosan (such as samples A, B) are poor in bactericidal properties, but their bactericidal value is also greater than 0, and also has certain antibacterial properties.

[Experimental Example 3] UV absorption effect test

In order to test the effect of the combination of chitosan and collagen on the absorption of ultraviolet rays, the UV absorption spectra of different formulations were compared in this experimental example.

Experimental procedure: 1. Preparation of sample a. A sample containing only chitosan (a solution containing 1% chitosan, the same chitosan from the previous experimental example was also used in this experimental example) b. Only added A sample of collagen (a solution containing 0.2% collagen, in this experimental example, the same source as the previous experimental example (fish scale collagen peptide (protein))) was used. c. Chitosan/collagen was added at the same time. A sample of protein (a solution containing 1% chitosan solution and 0.2% collagen solution) 2. The degree of absorption of UV in the range of about 200 nm to 400 nm was measured by a UV meter using a UV meter.

The experimental results are shown in Figures 3(a) to 3(d), respectively.

As shown in Fig. 3(a) to Fig. 3(d), the control group (Fig. 3(a)) did not have UV absorption, but the sample with only chitosan added (Fig. 3(b)) was not obvious. UV absorption. A sample in which only collagen was added (Fig. 3(c)) had absorption at a wavelength of around 280 nm (this is a UV absorption characteristic of the protein). However, the experimental group (Fig. 3(d)) to which chitosan and collagen were simultaneously added had absorption in the vicinity of 240 nm to 320 nm.

It can be seen from the present experimental examples that the composite formulation containing both chitosan and collagen does have UV absorption characteristics. It is speculated that this property may be caused by the interaction between chitosan and collagen, so samples containing only chitosan or collagen alone do not have UV absorption properties.

[Experimental Example 4] Animal Experimental Test

In order to further confirm the water-soluble chitosan and collagen egg of the present invention The actual efficacy of Baizhi spray was tested in this experiment on mature SD male rats, and compared with commercial products, it was confirmed whether it had better effect on wound healing.

Experimental steps:

(1) Mature SD male rats were administered by subcutaneous injection of 3.0 ml of a tranquilizer Rompun (trade name, manufactured by BAYER) and an anesthetic Zoletil (trade name, manufactured by Virbac) per kg;

(2) After the coma of the rat is confirmed, the hair of the back of the rat is shaved with an electric razor, and the initial temperature of the body surface is measured;

(3) A 2 second burn test was performed with a copper block at 90 ° C, and immediately after the sample was sprayed on the wound, the body surface temperature was measured again. By this step, the 1~2 grade of the back of the rat was burnt and sunburned (the opening was not opened).

(4) The chest and abdomen of the rat were wound with a commercially available bandage (made by 3M Company) to prevent the rat from biting the wound during the experiment, causing bacterial infection and damage to the shape of the wound.

(5) One day of observation and re-medication, the wound recovery was observed on the seventh day and the body temperature at the wound was measured.

Control group 1: Aloe vera water (commercial product: the product name is Aloe Vera Spray Water, manufactured by Guangyuanliang Co., Ltd.)

Control group 2: Nitrogen-filled compound formulation (ingredients containing 1% chitosan/0.2% collagen)

Experimental group: carbon dioxide gas-filled composite formula (ingredients containing 1% chitosan Sugar / 0.2% collagen)

The experimental results are shown in Figure 4. The black dashed box in the figure shows the location of the relatively obvious wound or scar tissue in the photo.

As shown in Fig. 4, there was no difference between the groups on the first day of the experiment, and there was no particularly obvious open wound on the back surface of the SD rats, but in fact, the cells which were burnt by the copper block in the body belonged to the shallow layer. Burnt burns.

On the 7th day, it was obvious that the wound healing of the control group 1 was poor, and there was still a large area of the unhealed area. However, although the wound healing of the control group 2 was better than that of the control group 1, there were still at least two deeper areas. Scar tissue. In contrast, the wound healing of the experimental group using the carbon dioxide gas-filled composite formulation was apparently better, almost restored without leaving deep scar tissue, and the scar portion was soft and prone to dislocation.

As is apparent from the present experimental examples, the spray containing the water-soluble chitosan and collagen of the present invention has a better effect on wound healing than the commercially available product.

In summary, the wound care composition provided by the present invention has the effects of antibacterial, hemostasis, and rapid cell proliferation, and can not only reduce the infection of the wound, but also absorb the ultraviolet light, thereby reducing the possibility of sunburn. After application, the wound can be quickly restored and the scar tissue can be reduced. In addition, the wound care spray of the present invention is obtained by filling a can with a gas containing carbon dioxide, and can improve blood circulation around the wound during application, and is effective. Promote wound healing. The wound care composition and spray of the present invention are also sufficiently convenient in use. Sexuality can meet the needs of health care such as hospitals and home care.

Although the present invention has been disclosed in the above embodiments, it is not intended to limit the present invention, and any one of ordinary skill in the art can make some changes and refinements without departing from the spirit and scope of the present invention. The scope of the invention is defined by the scope of the appended claims.

Claims (9)

A wound care composition comprising: a water-soluble chitosan having a degree of deacetylation of 80% or more; collagen; and an alcohol compound; wherein the collagen is 100% by weight based on the total weight of the above composition The protein content is 0.05% to 1%. The wound care composition according to claim 1, wherein the water-soluble chitosan has a molecular weight of from 100 kDa to 150 kDa. The wound care composition according to claim 1, wherein the pH value is in the range of 6.0 to 7.0. The composition for wound care according to claim 1, wherein the content of the water-soluble chitosan is 0.125% to 4% based on 100% of the total weight of the composition. The wound care composition according to claim 1, wherein the alcohol compound is at least one selected from the group consisting of ethanol, isopropanol, propanol and butanol. The wound care composition according to claim 1, wherein the content of the alcohol compound is 15% to 50% based on 100% by weight of the total composition. The wound care composition according to claim 1, wherein the content ratio of the water-soluble chitosan to the collagen is from 2:1 to 10:1. A wound care composition comprising: the wound care composition according to any one of claims 1 to 7; and a volatile carrier, wherein the volatile carrier is carbon dioxide gas. The wound care spray according to claim 8, wherein the ratio of the amount of the carbon dioxide gas to the volume of the composition solution is 1:1 to 1:2.