TWI458430B - Post-harvest disease controlling agent - Google Patents
Post-harvest disease controlling agent Download PDFInfo
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- TWI458430B TWI458430B TW096112281A TW96112281A TWI458430B TW I458430 B TWI458430 B TW I458430B TW 096112281 A TW096112281 A TW 096112281A TW 96112281 A TW96112281 A TW 96112281A TW I458430 B TWI458430 B TW I458430B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Description
本發明係關於採收後病害防除劑。The present invention relates to a disease control agent after harvesting.
為了防止採收後之農作物(尤其是果實等)腐敗而使用的採收後病害防除劑，以往已知碳酸氫鈉等鹼性鹽類可發揮效果。然而，其範圍僅限於柑橘之綠黴病或青黴病等特定之採收後病害(專利文獻1至4)。另外，有報告指出芥子油成分或β－檜酚酮(Hinokithiol)等揮發性殺菌成分亦具有採收後病害防除劑之效果(非專利文獻1至2)。再者，以往之農藥例如免賴得可濕性粉劑(Benlate wettable powder)、多保淨可濕性粉劑(Topsin wettable powder)等，亦被使用於防止採收後果實之腐敗。然而，此等為農藥，原本並不期望作為採收後使用者。In order to prevent post-harvest disease control agents used for the prevention of spoilage of crops (especially fruits, etc.) after harvesting, it has been conventionally known that alkaline salts such as sodium hydrogencarbonate can exert effects. However, the scope is limited to specific post-harvest diseases such as citrus green mold or penicillium (Patent Documents 1 to 4). In addition, it has been reported that a volatile bactericidal component such as a mustard oil component or a β-nonanolone has an effect of a post-harvest disease control agent (Non-Patent Documents 1 to 2). Further, conventional pesticides such as Benlate wettable powder and Topsin wettable powder are also used to prevent spoilage of fruits after harvesting. However, these are pesticides that were not originally intended as a post-harvest user.
現在所使用之採收後病害防除劑，以採收後之果實腐敗等所使用的防黴劑而言，有如依滅列(Imazalil)、鄰苯基酚、鄰苯基酚鈉、聯苯(Diphenyl)、噻苯咪唑(thiabendazal)等，但因可能有致癌性、致畸形性等危險性，故視為不佳(非專利文獻3)。The post-harvest disease control agent used today, such as the antifungal agent used for fruit spoilage after harvesting, is like Imazalil, o-phenylphenol, o-phenylphenol sodium, biphenyl ( Diphenyl), thiabendazal, etc., but may be considered to be poor because of the risk of carcinogenicity or teratogenicity (Non-Patent Document 3).
然而，使此等農藥附著於果實等，在食物安全上為不佳。此外，將採收時所產生之損傷傷口以熱進行烘燒亦與採收後病害之防除有關，但有作業繁雜且提高成本之問題。However, attaching such pesticides to fruits and the like is not good in food safety. In addition, the burning of the damaged wounds generated during harvesting is also related to the prevention of the diseases after harvesting, but there are problems in that the work is complicated and the cost is increased.
所以，需求一種對於多種採收後病害有效、從食品安全性之觀點而言亦無問題、而且廉價的採收後病害防除劑。Therefore, there is a need for a post-harvest disease control agent which is effective for a variety of post-harvest diseases, has no problem from the viewpoint of food safety, and is inexpensive.
[專利文獻1]日本特開昭50－111230號公報[專利文獻2]日本特開昭51－63932號公報[專利文獻3]日本專利第1168037號[專利文獻4]日本專利第1210679號[Patent Document 1] Japanese Laid-Open Patent Publication No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No.
[非專利文獻1]www.mfc.co.jp/wasaouro/wasaouro/index.html[非專利文獻2]www.fukuoka.com/nid/[非專利文獻3]www.pref.aichi.jp/shokuhinkensa/noyaku/boukabi.html[Non-Patent Document 1] www.mfc.co.jp/wasaouro/wasaouro/index.html [Non-Patent Document 2] www.fukuoka.com/nid/[Non-Patent Document 3] www.pref.aichi.jp/shokuhinkensa /noyaku/boukabi.html
本發明之目的係提供採收後病害防除劑，更詳細言之，係提供如香蕉冠腐病(Banana crown rot)、芒果蒂腐病(Mango stem－end rot)、木瓜蒂腐病(Papaya stem－end rot)等從採收時所產生之傷口引起感染的病害之防除劑。The object of the present invention is to provide a disease-preventing agent after harvesting, and more particularly to provide, for example, banana crown rot, Mango stem-end rot, papaya rot (Papaya stem) -end rot) A preventive agent for diseases that cause infection from wounds produced during harvesting.
本發明係提供以下所示之採收後病害防除劑。The present invention provides the post-harvest disease control agent shown below.
1.一種採收後病害防除劑，其含有由下述A群及B群所成群組中選出之至少1種成分：(A)甘油脂肪酸酯、山梨糖醇酐脂肪酸酯、山梨糖醇脂肪酸酯、或中鏈脂肪酸三酸甘油酯；(B)有機酸、或磷酸。A post-harvest disease control agent comprising at least one component selected from the group consisting of Group A and Group B: (A) glycerin fatty acid ester, sorbitan fatty acid ester, sorbose An alcohol fatty acid ester, or a medium chain fatty acid triglyceride; (B) an organic acid, or a phosphoric acid.
2.如上述1所記載之採收後病害防除劑，其中含有A群之至少1種成分及B群之至少1種成分者。2. The post-harvest disease control agent according to the above 1, wherein at least one component of the group A and at least one component of the group B are contained.
3.如上述1或2所記載之採收後病害防除劑，其中含有5至90質量份之A群成分及10至95質量份之B群成分者。3. The post-harvest disease controlling agent according to the above 1 or 2, which contains 5 to 90 parts by mass of the Group A component and 10 to 95 parts by mass of the Group B component.
4.如上述1至3中任一項所記載之採收後病害防除劑，其中，B群成分係己二酸、抗壞血酸、褐藻酸、檸檬酸、葡萄糖酸、琥珀酸、醋酸、酒石酸、乳酸、阿魏酸(ferulic acid)、富馬酸、丙酸、蘋果酸、或磷酸者。4. The post-harvest disease control agent according to any one of the above 1 to 3, wherein the component B is adipic acid, ascorbic acid, alginic acid, citric acid, gluconic acid, succinic acid, acetic acid, tartaric acid, lactic acid , ferulic acid, fumaric acid, propionic acid, malic acid, or phosphoric acid.
5.一種採收後病害防除劑，係將上述1至4中任一項所記載之採收後病害防除劑以水或植物油稀釋2至100倍而成者。A post-harvest disease control agent which is obtained by diluting the post-harvest disease control agent according to any one of the above 1 to 4 by water or vegetable oil by 2 to 100 times.
本發明之採收後病害防除劑，對於人體為安全，對於自然環境不會增加負擔，可具經濟性地且有效地防止採收後之果實腐敗。The post-harvest disease control agent of the present invention is safe for the human body and does not increase the burden on the natural environment, and can economically and effectively prevent the fruit from being corrupted after harvesting.
本發明之特徵係以可作為食品添加物利用的對於人或動物之安全性高的成分作為必須成分使用。The present invention is characterized in that a component which is highly safe for humans or animals which can be used as a food additive is used as an essential component.
本發明之A群成分係甘油脂肪酸酯、山梨糖醇酐脂肪酸酯、山梨糖醇脂肪酸酯、或中鏈脂肪酸三酸甘油酯。The group A component of the present invention is a glycerin fatty acid ester, a sorbitan fatty acid ester, a sorbitol fatty acid ester, or a medium chain fatty acid triglyceride.
A成分之甘油脂肪酸酯可為單酯、二酯、三酯中之任一者，成分脂肪酸之碳原子數以8至20為較佳，以12至18為更佳。甘油脂肪酸酯之具體例，可列舉如甘油單油酸酯(OL－100)、甘油單．二油酸酯(OL－200)、甘油二．三油酸酯(OL－95)等。The glycerin fatty acid ester of the component A may be any of a monoester, a diester or a triester, and the fatty acid of the component fatty acid is preferably from 8 to 20, more preferably from 12 to 18. Specific examples of the glycerin fatty acid esters include glycerol monooleate (OL-100) and glycerin alone. Dioleate (OL-200), glycerol II. Trioleate (OL-95) and the like.
A成分之山梨糖醇酐脂肪酸酯可為單酯、二酯、三酯、四酯中之任一者，成分脂肪酸之碳原子數以8至20為較佳，以12至18為更佳。其具體例可列舉如山梨糖醇酐月桂酸酯(L-250A、L-300)、山梨糖醇酐油酸酯(O-80)、山梨糖醇酐三油酸酯(OR-85)等。The sorbitan fatty acid ester of the component A may be any of a monoester, a diester, a triester or a tetraester, and the fatty acid of the component fatty acid is preferably from 8 to 20, more preferably from 12 to 18. . Specific examples thereof include sorbitan laurate (L-250A, L-300), sorbitan oleate (O-80), sorbitan trioleate (OR-85), and the like. .
A成分之山梨糖醇脂肪酸酯可為單酯、二酯、三酯、四酯中之任一者，成分脂肪酸之碳原子數以8至20為較佳，以12至18為更佳。其具體例可列舉如山梨糖醇之單、二或三月桂酸酯、山梨糖醇之單或二油酸酯等。The sorbitan fatty acid ester of the component A may be any of a monoester, a diester, a triester or a tetraester, and the constituent fatty acid preferably has 8 to 20 carbon atoms, more preferably 12 to 18 carbon atoms. Specific examples thereof include mono-, di- or trilaurate of sorbitol, mono or dioleate of sorbitol, and the like.
A成分之中鏈脂肪酸三酸甘油酯之成分中鏈脂肪酸之碳原子數以8至12較佳，以8至10為更佳。其具體例可列舉如C8單獨(M-2)、C8與C10之混合物(例如，60：40(M-1))等。The component of the chain fatty acid triglyceride in the component A has a carbon number of from 8 to 12, more preferably from 8 to 10. Specific examples thereof include C8 alone (M-2), a mixture of C8 and C10 (for example, 60:40 (M-1)), and the like.
本發明之B成分之有機酸，只要是對於人或動物不會造成有害影響者即無特別限制，但以可作為食品添加物使用之有機酸為較佳。較佳具體例可列舉如己二酸、抗壞血酸、褐藻酸、檸檬酸、葡萄糖酸、琥珀酸、醋酸、酒石酸、乳酸、阿魏酸、富馬酸、丙酸、蘋果酸等。The organic acid of the component B of the present invention is not particularly limited as long as it does not cause harmful effects to humans or animals, but an organic acid which can be used as a food additive is preferred. Preferred examples thereof include adipic acid, ascorbic acid, alginic acid, citric acid, gluconic acid, succinic acid, acetic acid, tartaric acid, lactic acid, ferulic acid, fumaric acid, propionic acid, malic acid and the like.
本發明之B成分以檸檬酸、琥珀酸、酒石酸、乳酸、蘋果酸、磷酸為特佳，以檸檬酸、琥珀酸、蘋果酸、磷酸為最佳。The component B of the present invention is particularly preferably citric acid, succinic acid, tartaric acid, lactic acid, malic acid or phosphoric acid, and preferably citric acid, succinic acid, malic acid or phosphoric acid.
本發明之採收後病害防除劑含有A成分與B成分中之至少1種，但較佳為含有A成分之至少1種及B成分之至少1種。The post-harvest disease control agent of the present invention contains at least one of the A component and the B component, but preferably contains at least one of the A component and at least one of the B component.
具體之採收後病害防除劑之較佳基本組成，例如可列舉如以下者：1.甘油脂肪酸酯＋有機酸2.山梨糖醇酐脂肪酸酯＋有機酸3.中鏈脂肪酸三酸甘油酯＋有機酸4.甘油脂肪酸酯＋山梨糖醇酐脂肪酸酯＋有機酸5.中鏈脂肪酸三酸甘油酯＋山梨糖醇酐脂肪酸酯＋有機酸6.甘油脂肪酸酯＋中鏈脂肪酸三酸甘油酯＋有機酸7.甘油脂肪酸酯＋山梨糖醇酐脂肪酸酯＋中鏈脂肪酸三酸甘油酯＋有機酸Specific preferred components of the post-harvest disease control agent include, for example, the following: 1. glycerin fatty acid ester + organic acid 2. sorbitan fatty acid ester + organic acid 3. medium chain fatty acid triglyceride Ester + organic acid 4. Glycerol fatty acid ester + sorbitan fatty acid ester + organic acid 5. Medium chain fatty acid triglyceride + sorbitan fatty acid ester + organic acid 6. Glycerol fatty acid ester + medium chain Fatty acid triglyceride + organic acid 7. Glycerol fatty acid ester + sorbitan fatty acid ester + medium chain fatty acid triglyceride + organic acid
當本發明之採收後病害防除劑含有成分A與成分B時，以成分A與成分B之合計作為100質量份，成分A之含量以5至90質量份為較佳，又以10至60質量份為更佳，尤以20至40質量份為最佳；殘餘部分為B成分。When the post-harvest disease control agent of the present invention contains the component A and the component B, the total of the component A and the component B is 100 parts by mass, and the component A is preferably 5 to 90 parts by mass, and further preferably 10 to 60. The mass fraction is more preferably, particularly preferably 20 to 40 parts by mass; the remainder is the component B.
本發明之採收後病害防除劑中，亦可含有除了成分A及/或成分B以外之其他具有界面活性功能之食品添加物(例如聚甘油脂肪酸酯等)等。The post-harvest disease control agent of the present invention may further contain a food additive (for example, a polyglycerin fatty acid ester or the like) having an interface activity function other than the component A and/or the component B.
本發明之採收後病害防除劑雖然可直接以原樣使用，但通常較理想為相對於該病害防除劑100質量份，添加較佳為30至10,000質量份、更佳為100至10,000質量份之水、植物油(例如椰子油、大豆油等)等，經攪拌、混合後使用。The post-harvest disease control agent of the present invention can be used as it is, but it is usually preferably added in an amount of from 30 to 10,000 parts by mass, more preferably from 100 to 10,000 parts by mass, per 100 parts by mass of the disease controlling agent. Water, vegetable oil (such as coconut oil, soybean oil, etc.), etc., are stirred and mixed and used.
本發明之採收後病害防除劑之對象病害，可列舉如香蕉冠腐病、芒果蒂腐病、木瓜蒂腐病等從採收時所產生之傷口引起感染的病害。The target diseases of the post-harvest disease control agent of the present invention include diseases such as banana crown rot, mango rot, and papaya rot, which cause infection from wounds generated at the time of harvesting.
本發明之採收後病害防除劑可稀釋於上述之溶媒或不經稀釋，藉由適當之方法(例如刷毛塗佈、噴霧塗佈)使附著於對象植物之期望部位。雖然其量可適當決定，但通常A成分及B成分之適當合計量以0.1至1,000g/m2 為較佳，以1至100 g/m2 左右為更佳。The post-harvest disease control agent of the present invention can be diluted to the above-mentioned solvent or undiluted, and adhered to a desired portion of the target plant by an appropriate method (for example, brush coating or spray coating). Although the amount thereof can be appropriately determined, a suitable total amount of the component A and the component B is preferably 0.1 to 1,000 g/m 2 , more preferably about 1 to 100 g/m 2 .
以下，以實施例更具體說明本發明。使用之成分(A)係如下述所示。Hereinafter, the present invention will be more specifically described by way of examples. The component (A) used is as follows.
Actor M－1：甘油三辛酸酯(Tricaprylin)(70質量%)/甘油三癸酸酯(tricaprin)(30質量%)Actor M－2：甘油三辛酸酯Rikemal L－250A：山梨糖醇酐月桂酸酯Rikemal L－300：山梨糖醇酐月桂酸酯Rikemal OL－100：甘油單油酸酯以上皆為理研維他命股份公司製品。Actor M-1: Tricaprylin (70% by mass) / triclorin (30% by mass) Actor M-2: Triacylglycerol Rikemal L-250A: Sorbitol Anhydride laurate Rikemal L-300: sorbitan laurate Rikemal OL-100: Glycerol monooleate is a product of Riken Vitamin Co., Ltd.
將Rikemal L－300與檸檬酸以質量比1：3混合。使用時，將此混合物1質量份以水1質量份揉合(knead)。Rikemal L-300 was mixed with citric acid at a mass ratio of 1:3. In use, 1 part by mass of this mixture was kneaded in 1 part by mass of water.
將Actor M－2與琥珀酸以質量比2：3混合。使用時，將此混合物1質量份以水2質量份揉合。Mix Actor M-2 with succinic acid at a mass ratio of 2:3. In use, 1 part by mass of this mixture was kneaded in 2 parts by mass of water.
將Actor M－1與蘋果酸以質量比3：3混合。使用時，將此混合物1質量份以水3質量份揉合。Mix Actor M-1 with malic acid at a mass ratio of 3:3. In use, 1 part by mass of this mixture was kneaded in 3 parts by mass of water.
將Rikemal L－300與Rikemal OL－100與檸檬酸以質量比1：1：3混合。使用時，將此混合物1質量份以水1質量份揉合。Rikemal L-300 was mixed with Rikemal OL-100 and citric acid at a mass ratio of 1:1:3. In use, 1 part by mass of this mixture was kneaded with 1 part by mass of water.
單獨使用Rikemal L－300。Use the Rikemal L-300 alone.
單獨使用檸檬酸。使用時，相對於檸檬酸1質量份以水3質量份揉合。Citric acid is used alone. In use, it was kneaded with 3 parts by mass of water with respect to 1 part by mass of citric acid.
單獨使用Actor M－1。Use Actor M-1 alone.
單獨使用Rikemal OL－100。Use Rikemal OL-100 alone.
單獨使用蘋果酸。Use malic acid alone.
削下市售香蕉之冠部，製作新的損傷面。在該損傷面上以採收後病害防除劑之有效成分量為10g/m2 塗佈實施例1至9之採收後病害防除劑。將無處理區設為對照組。然後，將用PDA培養基培養之Lasiodiplodia theobromae (黑腐病菌)以整個培養基貼附於切口上，保持於27℃之濕室(moist chamber)使其發病。7日後調查發病程度，從發病程度求得防除效價。採收後病害防除劑對於香蕉果梗部Lasiodiplodia theobromae 之生長與果梗部黑變之效果示於表1。Cut off the crown of the commercially available banana and make a new damaged surface. The post-harvest disease control agents of Examples 1 to 9 were applied to the damaged surface with the active ingredient amount of the post-harvest disease control agent of 10 g/m 2 . The no treatment zone was set as the control group. Then, Lasiodiplodia theobromae (black rot) cultured in PDA medium was attached to the incision with the entire medium, and kept in a moisture chamber at 27 ° C to cause disease. After 7 days, the incidence of the disease was investigated, and the titer was determined from the degree of the disease. The effect of the disease control agent after harvesting on the growth of the banana fruit stem portion Lasiodiplodia theobromae and the blackening of the fruit stem was shown in Table 1.
果實之變化 0：果梗無變色1：果梗之一部分為褐色2：果梗之一部分為黑色3：果實之一部分為黑色 Fruit change 0: Fruit stem without discoloration 1: Part of the fruit stem is brown 2: One part of the fruit stem is black 3: One part of the fruit is black
於損傷面上之菌絲生長 0：無菌絲生長1：僅可確認微量之生長2：可確認菌絲之生長3：菌絲茂盛地生長 Mycelial growth on the damaged surface 0: Sterile silk growth 1: Only trace growth can be confirmed 2: Growth of hyphae can be confirmed 3: Mycelium grows lushly
發病程度＝(0×n 0 ＋1×n 1 ＋2×n 2 ＋3×n 3 )/3N n0 ：程度為0之果實數n1 ：程度為1之果實數n2 ：程度為2之果實數n3 ：程度為3之果實數N：全果實數 The degree of disease = (0 × n 0 +1 × n 1 + 2 × n 2 + 3 × n 3 ) / 3N n 0 : the number of fruits with a degree of 0 n 1 : the number of fruits with a degree of 1 n 2 : the number of fruits with a degree of 2 n 3 : number of fruits of degree 3: total number of fruits
防除效價(%)＝(1－處理區之發病程度/無處理區之發病程度)×100Controlling the potency (%) = (1 - the degree of disease in the treatment area / the degree of disease in the no treatment area) × 100
削下市售香蕉之冠部，製作新的損傷面。在該損傷面上以採收後病害防除劑之有效成分量為10g/m2 塗佈實施例1、5及6之製劑。將無處理區設為對照組。然後，將用PDA培養基培養之表2及表3所示病原菌以整個培養基貼附於切口上，保持於25℃之濕室使其發病。7日後調查發病程度。對於香蕉果梗部因冠腐病相關病原菌而造成之果梗部黑變的效果示於表2，對於香蕉果梗部之冠腐病相關病原菌的生長抑制效果示於表3。Cut off the crown of the commercially available banana and make a new damaged surface. On the damaged surface, the preparations of Examples 1, 5 and 6 were applied in an amount of 10 g/m 2 of the active ingredient of the post-harvest disease control agent. The no treatment zone was set as the control group. Then, the pathogens shown in Tables 2 and 3 cultured in the PDA medium were attached to the incision with the entire medium, and kept in a humid chamber at 25 ° C to cause disease. The extent of the disease was investigated after 7 days. The effect of the blackening of the fruit stems caused by the pathogens related to the crown rot of banana was shown in Table 2. The growth inhibitory effect on the pathogens related to the crown rot of the banana fruit stem was shown in Table 3.
削下市售木瓜之果梗部分，製作新的損傷面。在該損傷面上以採收後病害防除劑之有效成分量為15g/m2 塗佈實施例1、4至6之製劑。將無處理區設為對照組。然後，將用PDA培養基培養之Lasiodiplodia theobromae 以整個培養基貼附於切口上，保持於25℃之濕室使其發病。7日後調查發病程度(果實之腐敗)。Cut off the fruit stalk portion of the commercially available papaya and make a new damaged surface. The preparations of Examples 1, 4 to 6 were applied to the damaged surface in an amount of 15 g/m 2 of the active ingredient of the post-harvest disease control agent. The no treatment zone was set as the control group. Then, Lasiodiplodia theobromae cultured in PDA medium was attached to the incision with the entire medium, and kept in a humid chamber at 25 ° C to cause disease. The extent of the disease (corruption of the fruit) was investigated after 7 days.
削下市售芒果之果梗部分，製作新的損傷面。在該損傷面上以採收後病害防除劑之有效成分量為15g/m2 塗佈實施例1、4至6之製劑。將無處理區設為對照組。Cut off the fruit stalk portion of the commercially available mango and make a new damaged surface. The preparations of Examples 1, 4 to 6 were applied to the damaged surface in an amount of 15 g/m 2 of the active ingredient of the post-harvest disease control agent. The no treatment zone was set as the control group.
然後，將用PDA培養基培養之Lasiodiplodia theobromae 以整個培養基貼附於切口上，保持於25℃之濕室，使其發病。7日後調查發病程度(果實之腐敗)。Then, Lasiodiplodia theobromae cultured in PDA medium was attached to the incision with the entire medium, and kept in a humid chamber at 25 ° C to cause disease. The extent of the disease (corruption of the fruit) was investigated after 7 days.
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| PCT/JP2007/057850 WO2007117003A1 (en) | 2006-04-07 | 2007-04-09 | Post-harvest disease controlling agent |
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| JP2017165654A (en) * | 2014-07-31 | 2017-09-21 | 国立研究開発法人理化学研究所 | Post harvest disease control agent and post harvest disease control method |
| ES2705378A1 (en) * | 2017-09-22 | 2019-03-22 | Decco Worldwide Post Harvest Holdings B V | METHOD FOR THE TREATMENT AND CONTROL OF POSTHARVEST PHYSITISTICS OF FRUITS THROUGH EDIBLE COATINGS. (Machine-translation by Google Translate, not legally binding) |
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| WO2001043549A2 (en) * | 1999-11-24 | 2001-06-21 | 3M Innovative Properties Company | Fruit, vegetable, and seed disinfectants |
| JP2002294286A (en) * | 2001-03-29 | 2002-10-09 | Teepol Ltd | Bactericidal detergent composition |
| JP2002370908A (en) * | 2001-06-12 | 2002-12-24 | Osaka Institute Of Environmental Life Science Inc | Preservative |
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| JP2866395B2 (en) * | 1989-08-04 | 1999-03-08 | 株式会社中埜酢店 | Sterilizing composition |
| JPH0953093A (en) * | 1995-08-10 | 1997-02-25 | Lion Corp | Detergent composition suitable for vegetable, fruit and the like |
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| WO2001043549A2 (en) * | 1999-11-24 | 2001-06-21 | 3M Innovative Properties Company | Fruit, vegetable, and seed disinfectants |
| JP2002294286A (en) * | 2001-03-29 | 2002-10-09 | Teepol Ltd | Bactericidal detergent composition |
| JP2002370908A (en) * | 2001-06-12 | 2002-12-24 | Osaka Institute Of Environmental Life Science Inc | Preservative |
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| TW200808175A (en) | 2008-02-16 |
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