TW200939956A - Agent for controlling post-harvest disease and method for controlling post-harvest disease - Google Patents

Abstract

The present invention provides an agent for controlling post-harvest disease and a method for controlling post-harvest disease. The present invention relates to an agent for controlling post-harvest disease and a method for controlling post-harvest disease using the same. This agent contains a liquid component as an active ingredient, the liquid component being obtained by mixing component (A), citric acid and ethanol, and heating the mixture until dissolved, then cooled.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an agent and a method for preventing postharvest disease caused by infection of agricultural products and fruits at the time of harvest. [Prior Art] In order to prevent spoilage of agricultural products after harvest, especially fruits, etc., it is known that a preparation such as sodium bicarbonate or the like is effective in a preparation for preventing post-harvest diseases. However, the scope thereof is limited to the post-harvest diseases specific to citrus green mold or green disease (Patent Documents 1 to 4). In addition, it has been reported that a volatile bactericidal component such as a mustard oil component or a hinokitiol also has an effect as a preparation for preventing post-harvest diseases (Non-Patent Documents 1 to 2). Further, conventional pesticides, for example, Benlate wettable powder, Topsin wettable powder, and the like are also used to prevent post-harvest fruit from being spoiled. However, these are pesticides that were not expected to be used after harvest. Imazalil, o-phenylphenol, o-phenyl group, which are used to prevent post-harvest diseases, are used as antifungal agents for fruit after harvest, such as spoilage. Phenol sodium, biphenyl (diphenyl), thiabendazole, etc. are considered to be poor because of their risk of carcinogenicity or fusiformity (Non-Patent Document 3). However, the attachment of such pesticides to fruits and the like is not good in food safety. Furthermore, although the damage to the damaged wounds produced at the time of harvesting is also related to the prevention of post-harvest diseases, there are problems in that the operation is complicated and the cost of the proposal is 321137, 3 200939956. Therefore, there is a need for a preparation which is effective for a variety of post-harvest diseases, has no problem from the viewpoint of food safety, and is inexpensive to prevent post-harvest diseases. [Patent Document 1] Japanese Laid-Open Patent Publication No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. No. Non-Patent Document 1] www.mfc.co.jp/wasaouro/wasaouro/index.html [Non-Patent Document 2] www.fukuoka.com/nid/ [Non-Patent Document 3] www.pref.aichi. jp/shokuhinkensa/ Noyaku/boukabi. html [Summary of the Invention] (Problems to be Solved by the Invention) An object of the present invention is to provide a preparation for preventing post-harvest diseases. Another object of the invention is to provide a method of preventing post-harvest diseases. (Method for Solving the Problem) The present inventors have found that citric acid has a south prevention effect on banana crown rot (Banana cr〇wn rot) and found that it can be further improved by coating citric acid with sorbitan fatty acid ester or the like. Prevent the effect. The present inventors further studied and found that when sorbitan fatty acid ester or the like is coated with citric acid to prevent the number of 1 m (especially (4)), the mixture is heated to a temperature at which the acid is melted and the water is obtained. The form of the product is significantly increased 321137 4 200939956 The effect of preventing banana crown rot and the like is completed, and thus the present invention has been completed. The present invention provides a preparation for preventing post-harvest diseases and a method for preventing post-harvest diseases as shown below. 1. A preparation for preventing post-harvest diseases, which contains the following liquid component as an active ingredient, and is selected from the group consisting of glycerin fatty acid ester, polyglycerin fatty acid ester, and sorbitan fatty acid vinegar (s〇) At least one component of a group consisting of rbitan fatty acid ester, s〇rMt〇1 fatty acid ester, and fatty acid triglyceride (A) a liquid component obtained by mixing with citric acid and an alcohol having 1 to 3 carbon atoms, heating, melting, and cooling. 2. The preparation for preventing post-harvest diseases according to the above 1, wherein the component (A) is used in an amount of 5 to 40 parts by mass, and the carbon number of 1 to 3 is used in an amount of 3 to 40 parts by mass based on 100 parts by mass of the citric acid. 3. The preparation for preventing post-harvest diseases according to the above 1 or 2, wherein the ingredient (A) is selected from the group consisting of glycerin fatty acid esters, polyglycerin fatty acid esters, and sorbitan fatty acid esters. At least one. 4. The preparation for preventing post-harvest diseases as described in any one of the above 1 to 3 wherein the alcohol having 1 to 3 carbon atoms is ethanol. A method for preventing a disease after harvesting, which comprises applying a preparation for preventing post-harvest diseases according to any one of items 1 to 4 above to a cut surface of a harvested fruit. 6. The method for preventing post-harvest diseases as described in the above 5, wherein the target disease is banana crown rot, Mango stem-end rot, or wood 5 321137 , 200939956 瓜蒂腐病 (Papaya stem-end) Rot). (Effects of the Invention) The preparations and methods for preventing post-harvest diseases of the present invention are safe for the human body, do not increase the burden on the natural environment, and can economically and effectively prevent fruit spoilage after harvest. [Embodiment] The present invention is characterized in that a component which can be used as a food additive and which is highly safe for humans and animals is used as an essential component. The component (A) of the present invention is a glycerin fatty acid ester, a polyglycerin fatty acid ester, a sorbitan fatty acid ester, a sorbitol fatty acid ester, or a three-chain fatty acid glycerin. The glycerin fatty acid ester of the component A may be any of a monoester, a diester or a triester, and the constituent fatty acid preferably has a carbon number of 8 to 20, more preferably 12 to 18. Specific examples of the glycerin fatty acid ester include glycerol monooleate (manufactured by Riken Vitamin Co., Ltd.: trade name: Rikemal OL-100), and glycerol mono-dioleate (manufactured by Riken Vitamin Co., Ltd.: trade name Poem OL-200), glycerol dioleate (manufactured by Riken Vitamin Co., Ltd.: trade name OL-95). The polyglycerol fatty acid ester of the component A is an esterified product of polyglycerol and a fatty acid obtained by polymerizing 2 to 10 or so glycerol molecules, and the constituent fatty acid has a carbon number of 8 to 20, preferably 12 to 18 For better. Specific examples of the polyglycerin fatty acid ester include diglycerin/laurate, diglycerin/stearic acid, diglycerin/oleic acid vinegar, triglycerin/mono-dioleic acid vinegar, and tetraglycerin/barium stearate. Detox, glycerol/lauric acid vinegar, decaglycerin/stearic acid vinegar, decaglycerin/oleic acid 6 321137 200939956 ester, polyglycerol/phthalic acid ester, polyglycerol/polyricinoleate, and the like. The sorbitan fatty acid ester of the component A may be any of a monoester, a diester, a triester or a tetraester, and the constituent fatty acid preferably has a carbon number of 8 to 20, more preferably 12 to 18. Specific examples thereof include sorbitan laurate (manufactured by Riken Vitamin Co., Ltd.: trade name Rikemal L-250A) and sorbitan oleate (manufactured by Riken Vitamin Co., Ltd.: trade name Poem 0-80V ), sorbitan trioleate (manufactured by Riken Vitamin Co., Ltd.: trade name Rikemal OR-85). The sorbitol fatty acid ester of the component A may be any of a monoester, a diester, a triester, a tetraester, a pentaester or a hexaester, and the carbon number of the constituent fatty acid is preferably from 8 to 20. 12 to 18 is better. Specific examples thereof include sorbitan mono- or dilaurate (manufactured by Riken Vitamin Co., Ltd.: trade name Rikemal L-300), sorbitol mono- or distearate (Rituhide Vitamin Co., Ltd.) System: the product name is Rikemal S-300W) and so on. The carbon number of the chain fatty acid among the three medium-chain fatty acid glyceride components of the component A is preferably from 8 to 12, more preferably from 8 to 10. Specific examples thereof include a C8 alone (manufactured by Riken Vitamin Co., Ltd.: trade name Actor M-2), and a mixture of C8 and CIO (for example, 60:40 (manufactured by Riken Vitamin Co., Ltd.: trade name Actor M) -1)) and so on. The citric acid used in the present invention may be a monohydrate or an acid needle'. However, when an acid anhydride is used, it is preferred to use water in an amount sufficient to form a monohydrate. If the monohydrate is heated, it will melt. Citric acid is not harmful to humans and animals and is used as a food additive. The preparation for preventing post-harvest diseases of the present invention contains the following liquid component as 7321137 200939956 as an active ingredient: it will be selected from the group consisting of glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, sorbitol fatty acid ester And a liquid component obtained by mixing at least one component (A) in a group consisting of three medium-chain fatty acid glycerides, and citric acid and an alcohol having 1 to 3 carbon atoms, and heating and melting, followed by cooling. The mixing order of the component (A), citric acid and the alcohol having 1 to 3 carbon atoms is arbitrary, but after mixing, it is necessary to heat until the citric acid is melted. Heating may be carried out as long as the melting temperature of citric acid is 100 ° C or higher. Usually, it is heated to 100 ° (: to 105 ° C). When the mixture as a whole has melted and becomes watery, the heating is stopped and cooled. The water-like product thus obtained is stored in the refrigerator (4 ° C). Regarding the use ratio of the component (A), citric acid, and the alcohol having 1 to 3 carbon atoms, the component (A) is preferably 5 to 100 parts by mass based on 100 parts by mass of the citric acid. More preferably 5 to 50 parts by mass, most preferably 5 to 20 parts by mass; the alcohol having 1 to 3 carbon atoms is preferably 3 to 40 parts by mass, more preferably 5 to 30 parts by mass, most preferably 5 to 20 parts by mass. When the amount of the component (A) and the citric acid is outside the above range, the product becomes easy to be solidified. The alcohol having 1 to 3 carbon atoms is used to make the citric acid and the component (A) easily mixed, and is heated. During the process, it evaporates slowly and is almost completely removed from the product. However, when ethanol is used as the alcohol having 1 to 3 carbon atoms, it is not required to be completely removed. In addition, the alcohol having 1 to 3 carbon atoms can be enumerated. Such as sterol, ethanol, and propanol. However, from the use as a post-harvest fruit, etc., to prevent post-harvest diseases. 8 321137 200939956 From the viewpoint of preparation, it is preferable to use a preparation for preventing post-harvest diseases of the present invention which is safe for use as a food-based safe, but it is also possible to add 100 parts by mass of the preparation. It is used to mix and mix water, 1 () ' 000 vegetable oil (such as coconut oil, soybean oil, etc.), etc., in a mass ratio (more preferably 100 to 10,000 parts by mass). ο The preparation for preventing post-harvest diseases of the present invention is used for preventing diseases caused by damage caused by fruits such as bananas, mangoes, papayas, oranges, avocados, and the like, and the specific diseases are caused by diseases. The banana crown rot, the mango rot, the papaya rot, the avocado rot, the fragrant preparation of the invention, the preparation for preventing post-harvest diseases can be diluted in the above-mentioned solvent and desired for the target plant without diluting the demand. ° According to the method of dicing (such as brush coating, spray coating) which is usually produced when the fruit is cut at the time of harvest or after harvesting, it is attached: ' 糸 is appropriately determined, but generally the prevention of the present invention : 5〇〇g/ More preferably, it is about 50 to 150 g/m2. (Examples) The present invention will be more specifically described below by way of examples. "When it is specifically indicated, it is f parts." *Other Example 1 The sorbitan fatty acid ester (sorbitol monolaurate, 5 parts) was dissolved in about 8%, and ethanol (2 parts) was added thereto, and 柃 = (10 parts) was added thereto, and the mixture was heated to 1 〇 5. 〇 and fully disturbed to make the whole melt. After removing the ethanol at '80 C·', a uniform liquid (waterwheel 321137 9 200939956) was prepared, and then cooled to room temperature. This liquid is watery even if it is cooled, and does not solidify at 4 °C. Example 2 5 parts of polyglycerin fatty acid ester (AG-7520: triglycerol mono-dioleate) was dissolved in 20 parts of ethanol with respect to 80 parts of citric acid, and heated at 100 ° C until the citric acid was dissolved. . Then, it was slowly cooled to room temperature. A mixture of citric acid and polyglycerin fatty acid ester which is also watery at room temperature is obtained. This liquid (watery) does not lose its flow even in the refrigerator (4 ° C). © Comparative Example 1 The sorbitan fatty acid ester (sorbitan laurate, 5 parts) was dissolved in 20 parts of ethanol at 50 ° C, and citric acid (80 parts) was added thereto at 50°. Coating was carried out while heating under C. The ethanol was removed by evaporation (warm air: about 60 ° C). The sorbitan sorbitan fatty acid ester coating agent is a powder having a slight moisture content. Comparative Example 2 5 parts of the polyglycerin fatty acid ester was dissolved in 20 parts of ethanol, 80 parts of citric acid was added thereto, and after sufficiently stirring, ethanol was removed by warm air (about 60 ° C) to obtain a coating agent. The coating agent is a slightly moisture-soluble powder. Test Example K Prevention effect of banana crown rot) Test method A commercially available banana was cut for each piece, and a new cut surface was made in the crown. Then, the preparations of Examples 1 and 2 were coated with 0. lg per 10 cm 2 of the cut surface, and the preparations of Comparative Examples 1 and 2 were combined with distilled water of the same quality 10 321 137 200939956, and coated for every 10 cm 2 of the cut surface. Lg as a control. Next, the crown rot cultured in the PDA medium was cut into a square having a length of about 5 mm from the entire PDA medium, and the mycelium surface was attached to the square medium in a manner of contacting the crown of the banana. Further, the area not treated with the drug is also inoculated. In each area, 5 bananas are used each. After the inoculation, the distilled water was dispersed to sufficiently wet the inoculated surface and the whole fruit, and stored in a wet room at 25 °C. After 7 days, the degree of occurrence of crown rot was observed. The extent of the disease is assessed based on the following assessment criteria. The results are shown in Table 0: No onset 0. 5: Signs of mild onset 1: Can confirm the onset 2: The lesion spreads to the crown as a whole 3: The lesion has exceeded the crown and reached the fruit [Table 1] Formulation Results Example 1 0 0 0 0. 5 0 Example 2 0 0 0 0 0 Comparative Example 1 1 1 0. 5 1 1 Comparative Example 2 1 0. 5 0. 5 0· 5 0 No treatment 3 3 3 3 3 It is understood that the preparation of the examples has an excellent preventive effect. Test Example 2 (Test for phytotoxicity of bananas) 11 321137 200939956 Test method Commercially available bananas were divided into one group for every three strips, and a new cut section was made for the crown. Then, the coatings of Examples 1 and 2 were coated with 0. lg per 10 cm 2 of the cut surface. Further, the preparations of Comparative Examples 1 and 2 were exemplified by distilled water of the same quality, and 〇·ig was applied per 1 cm 2 of the cut surface. The coating system is carried out in the crown and fruit portions. The area where the fruit was not coated with anything was set as a control. In each area, 5 bananas are used each. After treatment, it was stored in a 25 °C room, and after 7 days, the presence or absence of phytotoxicity and its extent were evaluated according to the following evaluation criteria. The results are shown in Table 2. 〇: no phytotoxicity 0.5: slight change in the coated surface of the drug 1: the coating site of the drug has changed color 2: the coating site and the surrounding area have changed color 3: the discoloration is obvious [Table 2] Preparation results - implementation Example 1 0 0 0. 5 0 ---_ 0 Example 2 0 0 0 0 ---- 0 Comparative Example 1 "0. 5 1. 0 0. 5 1.0 〇.^~ Comparative Example 2 0· 5 0.5 0.5 1.0 —-^ 0 5 No treatment 0 0 __ 0 0 --- 0 , it can be seen that the preparation of the application example hardly causes phytotoxicity. Example 3 321137 12 200939956 In Example 2, 90 parts by mass of citric anhydride In place of citric acid, 10 parts by mass of water was added thereto, and ethanol and polyglycerin fatty acid vinegar were added thereto, and heat treatment was carried out in the same manner. A water-like product similar to that of Example 2 was obtained. Comparative Example 3 In Example 1, The heat treatment was carried out in the same manner except that malic acid, phosphoric acid, or lactic acid was used instead of citric acid, but malic acid, phosphoric acid, or milk or acid did not dissolve, and the same water-like formation as in Example 2 could not be obtained.

Test Example 3 Using the preparations of Example 3 and Comparative Example 3, the prevention effect against banana crown rot was examined in the same manner as in Test Example 1. The preparation of Example 3 obtained the same preventive effect as the preparation of Example 2, but the preparation (3 types) of Comparative Example 3 was only able to obtain the same preventive effect as the preparation of Comparative Example 2. [Simple diagram description] No Q [Explanation of main component symbols] None 13 321137

Claims (1)

200939956 VII. Patent application scope: 1. A preparation for preventing post-harvest diseases, which comprises the following liquid component as an active ingredient: it is selected from the group consisting of glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, and sorbus a liquid obtained by mixing a sugar alcohol fatty acid ester and a medium medium chain fatty acid glyceride, at least one component w) with citric acid and an alcohol having 1 to 3 carbon atoms, and heating, melting, and cooling Ingredients. 2. The preparation for preventing post-harvest diseases according to the scope of the patent application, wherein 5 to 1 part by mass of the component (A) and 3 to 40 parts by mass of the carbon number are used with respect to 100 parts by mass of citric acid. ! To 3 alcohol. 3. In the case of applying for the post-harvest disease of the scope of the patent or the second item, the 'component' (A) is selected from the group consisting of glycerin fatty acid esters, polyglycerol fatty acid esters and sorbitan fatty acid esters. At least one of the groups. 4. A preparation for preventing post-harvest diseases as claimed in any of the scope of the patent application, wherein the alcohol having an amount of 1 to 3 is ethanol. 5. Two methods for preventing post-harvest diseases, which are methods for applying the anti-harvest disease of the application scope of the patent range i 3 = :: to the disease prevention post-harvest disease of item I == disease. It has no crown rot, mango rot, or papaya rot. 321137 14 200939956 * IV. Designated representative map: There is no schema in this case (1) The representative representative map of this case is: (). (2) A brief description of the symbol of the representative figure: 5. If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention: This case does not represent the chemical formula 321137