TWI383800B - Toona sinensis extract and extracting method thereof - Google Patents

Description

Citron extract and its extraction method

The present invention relates to a citron extract, in particular to a citron extract for use in regulating blood sugar.

Citron (alias 椿 or eucalyptus, scientific name Toona sinensis ) is a perennial deciduous hormonal plant native to southeast China, southwest to north China, and has been widely planted globally. There are few side branches, the bark is slightly white, the whole plant has a special smell, and its young leaves are edible and can be collected all year round, which is of great value.

Because the tree itself is beautiful, it is very suitable as a street tree or ornamental tree. Its wood can also be used to make furniture or building materials. In addition, since ancient times, there has been a saying that "food is good for chewing and not contaminated with miscellaneous diseases". In the traditional pharmacopoeia, there are also records of the benefits of eating cigarettes or using toon, including heat-clearing and detoxifying, stomach-smelling and hemostasis. Etc., both disclose the development potential of Shannon in medicine or health food. In addition, modern biological research has also shown through molecular or cellular experimental evidence that the use of camphor in cancer or tumor treatment is more effective, which further demonstrates the practical and economic benefits that can be provided by health care.

In addition to the treatments used in certain cancers, traditional practices or customary knowledge have been used to consume or drink cigarettes to achieve a blood sugar lowering practice. However, the general public only drink hot tea brewed with camphor tea or camphor tea powder. This kind of "Cinnamon Tea" is not only difficult to quantify in the proportion of toon ingredients, but also has no significant effect on lowering blood sugar, and even regulating or soothing the symptoms of metabolic syndrome (such as before the onset of diabetes symptoms) and its subsequent symptoms. The mechanism also lacks the support of any scientific theory. In addition, such conventional methods of using citron leaves have limited extraction of active ingredients for regulating blood sugar in citron, and it is difficult to further prepare them into health foods or pharmaceutical compositions.

Therefore, how to provide a citron extract which can be extracted by a specific preparation method to effectively obtain an active ingredient in citron leaves and has a good blood sugar regulating function has become one of important subjects.

In view of the above problems, it is an object of the present invention to provide a citron extract which can be extracted by a specific preparation method and which has a good blood sugar regulating function.

Another object of the present invention is to provide a process for preparing a camphor extract which can be used to prepare the above-described camphor extract.

To achieve the above object, a citron extract according to the present invention is prepared by the following steps comprising: placing a citron leaf in an aqueous alcohol-containing solution; and extracting to obtain an alcohol extraction mixture.

According to the citron extract of the present invention, in the preparation step, the citron leaf is a fragrant leaf powder or a fragrant leaf fragment. In one embodiment of the invention, the citron leaf is a fragrant green leaf powder.

According to the citron extract of the present invention, in the preparation step, the alcohol-containing aqueous solution has an alcohol content percentage by volume of 70% to 100%. In one embodiment of the invention, the alcohol-containing aqueous solution has an alcohol content volume percentage of 70%.

In one embodiment of the invention, the step of preparing the camphor extract of the present invention further comprises separating the alcohol extract mixture to obtain an alcohol extract and an alcohol extract.

In one embodiment of the invention, the step of preparing the camphor extract of the present invention further comprises drying or concentrating the alcohol extract.

In an embodiment of the present invention, the preparation step of the toon extract of the present invention further comprises taking an alcohol extract and extracting with water under high temperature and high pressure to obtain a water extraction mixture.

In one embodiment of the invention, the step of preparing the camphor extract of the present invention further comprises separating the water extraction mixture to obtain an aqueous extract.

In one embodiment of the present invention, the step of preparing the camphor extract of the present invention further comprises mixing the alcohol extract and the water extract.

According to the citron extract of the present invention, the mixing ratio of the alcohol extract and the water extract in the preparation step may be from 3:1 to 6:1. In one embodiment of the present invention, the volume ratio of the above-mentioned alcohol extract and water extract is 3:1.

In an embodiment of the present invention, the step of preparing the camphor extract of the present invention further comprises separately drying or concentrating the above-mentioned alcohol extract and water extract, and mixing.

According to the citron extract of the present invention, the ratio of the dried or concentrated alcohol extract and the dried or concentrated water extract in the preparation step is from 3:1 to 6:1. In one embodiment of the invention, the volume ratio of the dried or concentrated alcohol extract and the dried or concentrated water extract is 3:1.

A method of preparing a camphor extract according to the present invention comprises placing a vanilla leaf in an aqueous alcoholic solution; and extracting to obtain an alcohol extract mixture.

A citron extract according to the present invention, which is used for regulating blood sugar, is prepared by the following steps, comprising: placing a citron leaf in an aqueous solution containing an alcohol; and extracting to obtain an alcohol extraction mixture.

As described above, since the camphor extract according to the present invention uses an alcohol solution as an extraction solvent, the active ingredient in the camphor leaves can be effectively obtained. Furthermore, the citron extract obtained by the preparation step disclosed in the present invention can have a better blood sugar regulating ability and is suitable for further application in health foods and even medical fields.

Hereinafter, a citron extract according to the present invention will be described with reference to the related drawings, wherein the same elements will be described with the same reference numerals.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a flow chart showing the steps of preparing a citron extract according to a preferred embodiment of the present invention, and also a flow chart of the method for preparing a citron extract according to a preferred embodiment of the present invention. Referring to FIG. 1, a citron extract according to the present invention is prepared by the following steps, comprising: placing a citron leaf in an alcohol-containing aqueous solution (S10); and extracting to obtain an alcohol extraction mixture A1 ( S20).

In step S10, the citron leaf is a leaf collected from the citron (scientific name Toona sinensis ), and the type thereof may be, for example but not limited to, a fragrant leaf powder or a fragrant leaf shard. In this embodiment, the citron leaves are fragrant scorpion leaf pieces which are obtained by grinding, or mashing, or other means for achieving the same purpose. Because the fragrant leaf fragments or powder have a higher surface area to volume ratio than the intact citron leaves, the extraction efficiency can be effectively improved.

In this embodiment, the alcohol in the above alcohol-containing aqueous solution may be, for example but not limited to, methanol, or ethanol, or propanol, or isopropanol, or n-butanol, or isobutanol, or any combination thereof. The alcohol-containing aqueous solution is preferably an aqueous solution containing ethanol.

In addition, in order to effectively extract the active ingredient in the citron leaves, in the present embodiment, the alcohol content of the alcohol-containing aqueous solution accounts for 70% to 100% by volume of the total solution; preferably, the alcohol content accounts for the volume percentage of the total solution. It is 70%.

The method for extracting the citron leaves in the alcohol-containing aqueous solution is not particularly limited in practice, and is mainly for shaking, shaking, or oscillating, or any other similar means for the citron leaves in the aqueous solution containing alcohol to achieve the acquisition. The purpose of the alcohol extraction mixture A1 containing the citron active ingredient. It should be noted that whether it is shaking, shaking, or oscillating, or any other similar means, it can be performed manually, or semi-automatically, or automatically, and there is no particular limitation on the implementation. In the present embodiment, means suitable for achieving the above object may be, for example but not limited to, continuously or intermittently or periodically shaking or shaking a container containing an aqueous solution containing alcohol and citron leaves, or providing ultrasonic waves by machine or instrument. The citron leaves in the aqueous solution containing alcohol are oscillated over a period of time.

Therefore, the camphor leaves are placed in a specific proportion of an alcohol-containing water solution and extracted to obtain an alcohol extract mixture A1. The alcohol extraction mixture A1 obtained according to the present invention has a higher aroma active ingredient and a better blood sugar regulating ability than the conventional method of directly brewing the toon with hot water (for further explanation, please refer to Experimental Example 2).

Referring to FIG. 1 , in the present embodiment, after the step S20 of extracting the mixture A1 for alcohol extraction, a step of separating the alcohol extraction mixture A1 to obtain an alcohol extract A11 and an alcohol extract A12 is further included. (S30). In this step S30, the method for separating the alcohol extraction mixture A1 may be, for example, but not limited to, centrifuging the alcohol extraction mixture A1 to precipitate the alcohol extract A12 to be separated from the alcohol extract A11; or separating the alcohol by filtration. Extract A12 and alcohol extract A11. In the present embodiment, the alcohol extract A12 and the alcohol extract A11 are separated by centrifugation, the alcohol extract A11 is the supernatant, and the alcohol extract A12 is the residue deposited on the bottom or side wall of the vessel. In addition, the number of revolutions and the length of time used for centrifugation can be adjusted depending on the actual implementation, and is not particularly limited.

Referring to FIG. 1 , in the present embodiment, after the step S30 of obtaining the alcohol extract A11 and the alcohol extract A12, an alcohol extract A12 is further included, and the water is extracted under high temperature and high pressure to obtain a water extraction. The step of the mixture H1 (S40). Specifically, in this step, it is preferred to take an alcohol extract and extract it with water at a temperature of 121 ° C and a pressure of 1 kgw / cm ² for 10 to 30 minutes to obtain a water-extraction mixture H1.

Referring to FIG. 1 , in the present embodiment, after the step S40 of extracting the mixture H1 with water under high temperature and high pressure, the step of extracting the mixture H1 with water is further included to obtain a water extract H11. (S50). In the step S50, the method for separating the water extraction mixture H1, and/or the setting conditions, and/or the device may refer to the separation of the alcohol extraction mixture A1 in the step S30, and details are not described herein again. It should be noted that the implementation still pays attention to the difference between alcohol and water and then make appropriate adjustments.

Referring to FIG. 1, in the present embodiment, after the step S50 of obtaining the aqueous extract H11, a step (S60) of separately drying or concentrating the alcohol extract A11 and the water extract H11 and mixing them is further included. By treatment in step S60, a dried or concentrated alcoholic extract and a dried or concentrated aqueous extract are obtained. In step S60, the method of drying or concentrating the alcohol extract A11 and the water extract H11 may be, for example but not limited to, evaporation, or lyophilization, or spray-drying, heating. Concentrated, or any combination thereof. In the present embodiment, the alcohol extract A11 and the water extract H11 were treated by freeze drying. However, it is particularly emphasized that the drying or concentration treatment of the alcohol extract A11 and the water extract H11 does not have to be completely dried or the liquid contained therein is completely removed, which may be adjusted according to actual needs, in the sense that This is because the proportion of the liquid in the extract is reduced in the overall proportion of the solid-liquid mixture. In other words, the dried or concentrated alcoholic extract and the dried or concentrated aqueous extract may be present in various forms, such as a dried powder or crystallization, or an incompletely dried powder, or a viscous liquid, or a concentrated alcoholic extract A11. And water extract H11.

Further, in order to make the camphor extract of the present invention have a better action activity, the mixing ratio of the dried or concentrated alcohol extract and the dried or concentrated water extract may be 3:1 to 6 in step S60: 1. In this embodiment, the mixing ratio is 3:1. After mixing, a citron extract M having a better blending effect of the active ingredients of the citron is obtained.

However, it should be emphasized again that although the best extract of the citron extract of the present invention can be obtained through the above steps S10 to S60 (for further explanation, please refer to Experimental Example 2), it is not limited to the steps of the citron extract of the present invention. It was completed. On the contrary, after the steps S10 and S20 are performed, the steps S30 to S60 disclosed in the preferred embodiment of the present invention are performed in the middle or after the completion, or from the step S30 to the step S60, or after the process is completed. The resulting extract, or mixture, or mixture, or any combination thereof, in one or several steps is also encompassed within the scope of the extracts of the present invention. The following will be exemplified.

Referring to FIG. 1 , in another aspect of the embodiment, after the step S30 of obtaining the alcohol extract A11 and the alcohol extract A12, the step of drying or concentrating the alcohol extract A11 may be further included (S41). ). In the step S41, the drying or concentration treatment is the same as the drying or concentration treatment described in the step S60, and details are not described herein again. The dried or concentrated alcohol extract can also be obtained after this step S41. However, although it is the same as the dried or concentrated alcohol extract obtained in the step S60, it does not need to be mixed with other extracts or extracts, and the tea powder which is similar to the conventional hot water brewing is preferably active. .

Similarly, referring to FIG. 1 , in another aspect of the embodiment, after the step S50 of obtaining the water extract H11, the step of further mixing the alcohol extract A11 and the water extract H11 (S61) . After the step S61, a mixed extract can be obtained, which is also a kind of the extract of the camphor extract obtained according to the present invention, and also has the active effect of the hot tea brewed with the known hot water. Of course, in order to increase the activity of the extract of the camphor extract in the mixed extract, in the present embodiment, the mixing ratio of the alcohol extract A11 and the water extract H11 may be from 3:1 to 6:1, and preferably. The mixing ratio is 3:1.

In summary, a citron extract according to the present invention uses an alcohol solution as an extraction solvent to effectively obtain an active ingredient in citron leaves. Furthermore, the citron extract obtained by the preparation step disclosed in the present invention can have a better blood sugar regulating ability and is suitable for further application in health foods and even medical fields. Further, in the examples, the obtained products are mixed in a specific ratio after the extraction, and the effect of the active ingredients in the camphor extract can also be increased.

The present invention also discloses a citron extract for regulating blood sugar, and the citron extract is prepared by the following steps, comprising: placing a citron leaf in an aqueous solution containing alcohol; and extracting to obtain an alcohol extraction mixture. .

In addition, the present invention further discloses a citron extract which is applied to increase the activity of peroxisome proliferators activated receptor-gamma (PPAR-γ), and the citron extract is prepared through the following steps. , comprising placing a citron leaf in an aqueous solution containing alcohol; and extracting to obtain an alcohol extraction mixture. The means, and/or manner, and/or the solution, and/or the apparatus used in the preparation method of the above two kinds of toona sinensis extracts, and the means and/or manners described in the above-exemplified examples, and / or the solution, and / or the device is the same, and will not be described here.

In the prior art, it is known that the activation of peroxisome proliferator-activated receptor-γ has the effect of enhancing the sensitivity of cells to insulin, and can thereby reduce blood glucose concentration by machine. The extract of the citronella disclosed in the present invention has the ability to enhance the activity of the peroxisome proliferator-activated receptor-γ (for further explanation, please refer to Experimental Example 2), and therefore, the camphor extract of the present invention has a modulation into a health food or The potential of the beverage, and depending on the selected extract form, such as a dry powder or extract, can be solidified or processed in a solid or liquid form as desired in accordance with the teachings of the art in the art. In addition, a suitable supplement/agent, and/or flavoring agent, and/or buffer, and/or stabilizer, and/or the like may be optionally included to determine the health food or beverage in the camphor extract. Other characteristics than the active efficacy. The health food or drink obtained according to the present invention not only has better activity than the known citron tea, but also facilitates commercialization and popularization. For example, in one non-limiting embodiment of the invention, when the citron extract of the present invention is formulated into an oral liquid preparation, the stabilizer used is tragacanth, and/or arabic. Gum arabic and / or gelatin, as the buffer used is sodium citrate.

The invention further discloses a pharmaceutical composition for regulating blood sugar of a camphor extract comprising an effective amount of a camphor extract selected from any of the various extracts of the above embodiments. Furthermore, the pharmaceutical composition may further comprise a pharmaceutically acceptable carrier. Wherein, the term "carrier" includes any carrier conventional in the art, and may be, for example but not limited to, water, or physiological saline, or glycerin, or an organic solvent, or a stabilizer, or a chelate. Mixtures, or diluents, or preservatives, or emulsifiers, or suspensions, or gels, or liposomes, and the like. Additionally, in one embodiment of the invention, the pharmaceutical composition further comprises an excipient. Accordingly, the pharmaceutical composition is prepared as an oral solid preparation of the type comprising a troche, or a coated lozenge, or granules, or a powder, or a capsule, or the like.

Furthermore, in addition to being suitable for oral administration, the pharmaceutical compositions disclosed herein can also be prepared by parenteral, injection or topical administration according to conventional techniques in the art of the present invention. Pharmaceutical composition.

Several experimental examples will be enumerated below to illustrate the preparation steps of the camphor extract according to the preferred embodiment of the present invention and the effect of increasing the activity of the peroxisome proliferator-activated receptor-gamma.

Experimental Example 1. Preparation steps of Toona sinensis extract

A preparation step of the camphor extract of the present invention will be described below by way of an experimental example.

One kilogram of the fragrant green leaf pieces was soaked in 10 liters of 70% ethanol solution, and the fragrant sage leaf pieces were shaken at 150 rpm for 10 hours at room temperature to obtain an alcohol extraction mixture A1. After extracting the mixture A1 at 10,000 x g for about 20 minutes, the alcohol extract A11 (supernatant) and the alcohol extract A12 (fragrant green leaf residue) were separated. The centrifuged alcohol extract A12 was further added to 4 liters of water, and extracted at a temperature of 121 ° C and a pressure of 1 kgw / cm ² for 20 minutes to obtain a water extraction mixture H1, and then the mixture was extracted with 10,000 X g of centrifugal water. The aqueous extract H11 (supernatant) was separated by about 20 minutes. Then, the alcohol extract A11 and the water extract H11 are separately subjected to freeze-drying treatment to obtain a dried alcohol extract and a dried aqueous extract. Finally, the above dried alcohol extract and the dried aqueous extract were mixed in a ratio of 3:1 to form a mixed camphor extract M1.

Experimental Example 2. Increasing the Peroxisome Activity of Peroxidase Promoting Receptor-γ Activity by Toona sinensis Extract

(a) cell processing

Human hepatoma cell lines (HepaG2 cells) were cultured in advance in a 12-well plate according to a conventional technique of cell culture. Thereafter, solution A and solution B were placed in each well. Among them, solution A is 175 μl of egg-free basic medium (FBS free DMEM) containing no fetal bovine serum containing 0.4 μg of carrier pG5luc and 0.2 μg of carrier pBPPARγ, and solution B is containing 4 μg of liposome 2000 (lipofectamine). 2000) 175 μl of Eagle's basic ingredient medium without fetal bovine serum. The solution A and the solution B were mixed and allowed to stand for 40 minutes (solution A and solution B were individually prepared before mixing). The mixed solution A and solution B were separately added to the cells in a 12-well culture dish, and cultured for 24 hours, and then the medium was changed to a normal Eagle-based basic medium by a conventional method, and cultured for another 24 hours. . Then, a piric acid solution (pioglitazone, a drug that acts as a conjugate of peroxisome proliferator-activated receptor-γ) with activated peroxisome proliferator-activated receptor-γ is added to each well in the culture dish. The effect can promote the metabolism of blood sugar in the body, thereby lowering the blood sugar concentration), or the dried tea of the camphor tea which is brewed by hot water (the first is to brew the camphor tea with hot water of 100 ° C for 30 minutes, then centrifuge to take the supernatant. , or lyophilized, or a solution of the dried or concentrated alcohol extract, or a solution of the dried or concentrated aqueous extract, or a mixed solution of the camphor extract M1, to be cultured in a cell culture incubator Cold light analysis was performed after 24 hours.

(2) Luminescence analysis

The vector used for transfecting cells is a reporter gene expressing a firefly luciferase gene, and its expression is regulated by peroxisome proliferator-activated receptor-γ activity. Therefore, by analyzing the expression amount of firefly luciferase by the conventional technique in the field of the present invention, the activity of intracellular peroxisome proliferator-activated receptor-γ can be determined.

Accordingly, 250 μl of a cell lysis buffer was added to each well of the culture dish. After the cells were sufficiently dissolved, 10 μl of the cell lysate was taken out from each well and added to a firefly luciferase substrate. After mixing and reacting for a period of time, the luminescence value of the firefly luciferase was detected by a Mini-Lumat LB 9506 meter; then, the merion luciferase substrate was added to detect the jellyfish. The luminescence value exhibited by phototinase. Finally, the luminescence measurement value obtained by the action of firefly luciferase is divided by the luminescence measurement value expressed by the jellyfish luciferase and the blank control group (without adding any agent, extract, or extract) The cold light measurement value and the luminescence measurement value of the positive control group (with the addition of pirinone to a concentration of 0.1 μg/ml) were compared, and it was found that each citron extract or extract activated the peroxisome proliferator-activated receptor-γ. Strong and weak differences.

(III) Analysis of results

Adding the above-mentioned solution of the dried hot tea brewed camphor tea, and the dried or concentrated alcohol extract, and the dried or concentrated water extract solution, and the mixed camphor extract M1 solution Cold light tests related to the activity of peroxisome proliferator-activated receptor-gamma were performed separately.

Figure 2 is the luminescence intensity exhibited by the solution of the mixed extract of the camphor extract M1 at different concentrations, and Figure 3 is the luminescence intensity exhibited by the solution of the dried or concentrated aqueous extract at different concentrations, Figure 4 is a graph showing the luminescence intensity of a solution of a dried or concentrated alcohol extract at different concentrations, and Figure 5 is a graph showing the treatment of dried citron tea with different concentrations of hot water. Cold light intensity. Referring also to Figures 2 to 5, it is apparent that the camphor extract according to the present invention, whether it is a mixed camphor extract M1 (Figure 2), or a dried or concentrated alcohol extract (Figure 3), or dried Or the concentrated water extract (Fig. 4) has a higher activity of the peroxisome proliferator-activated receptor-γ activation than the hot water brewed citron tea dry (Fig. 5). If examined individually, the mixed camphor extract M1 in Figure 2 means that the dried alcohol extract and the dried water extract are mixed in a ratio of 3:1, which activates the peroxisome proliferator-activated receptor- γ is the best, about 110% of the positive control group. The dried or concentrated aqueous extract in Figure 3 activates the peroxisome proliferator-activated receptor-gamma with increasing dose, up to about 40% of the positive control group (with 0.1 μM piricone). effect. The dried or concentrated alcohol extract in Figure 4 activates the peroxisome proliferator-activated receptor-γ, which is better than the dried or concentrated aqueous extract, which is equivalent to about 60% of the positive control group. . As shown in Fig. 5, as for the dried citron tea which is conventionally brewed with hot water, it is only equivalent to about 40% of the activation effect of the positive control group. It is worth mentioning that the mixed camphor extract M1 in Fig. 2 has better effects than the dried or concentrated alcohol extract (Fig. 3) and the dried or concentrated water extract (Fig. 4), respectively.

The above is intended to be illustrative only and not limiting. Any equivalent modifications or alterations to the spirit and scope of the invention are intended to be included in the scope of the appended claims.

A1. . . Alcohol extraction mixture

A11. . . Alcohol extract

A12. . . Alcohol extract

H1. . . Water extraction mixture

H11. . . Water extract

M1. . . Mixed toon extract

S10~S61. . . step

1 is a flow chart showing the steps of preparing a camphor extract according to a preferred embodiment of the present invention;

Figures 2 to 5 are graphs showing the luminescence intensity data exhibited after treatment with various doses of the various extracts of the present scent and the dried citron tea of the present invention.

S10~S61. . . step

Claims (19)

A citron extract obtained by the following steps: placing a fragrant scented leaf in an aqueous alcohol solution; extracting to obtain an alcohol extraction mixture; separating the alcohol extraction mixture to obtain an alcohol extract and an alcohol The extract; and the alcohol extract is extracted with water at a high temperature and high pressure to obtain a water-extraction mixture. The citron extract according to claim 1, wherein the citron leaf is a fragrant leaf powder or a fragrant leaf fragment. The citron extract according to claim 1, wherein the alcohol-containing aqueous solution has an alcohol content percentage by volume of 70% to 100%. The citron extract according to claim 1, further comprising: separating the water extraction mixture to obtain an aqueous extract. The citron extract according to claim 4, further comprising: mixing the alcohol extract and the water extract. The citron extract according to claim 5, wherein the alcohol extract and the water extract are mixed in a ratio of from 3:1 to 6:1. The citron extract according to claim 4, further comprising: separately drying or concentrating the alcohol extract and the water extract, and mixing. The citron extract according to claim 7, wherein the dried or concentrated alcohol extract and the dried or concentrated aqueous extract are mixed in a ratio of from 3:1 to 6:1. A citron extract for use in regulating blood sugar, and prepared by the following steps, comprising: placing a fragrant scented leaf in an aqueous solution containing alcohol; extracting to obtain an alcohol extraction mixture; separating the alcohol extraction mixture to obtain a An alcohol extract and an alcohol extract; and the alcohol extract is extracted with water at a high temperature and high pressure to obtain a water-extraction mixture. A camphor extract for increasing the activity of a proliferator-activated receptor- gamma (PPAR-γ), which is prepared by the following steps, comprising: placing a vanilla leaf in an aqueous solution containing alcohol; extracting An alcohol extraction mixture is obtained; the alcohol extraction mixture is separated to obtain an alcohol extract and an alcohol extract; and the alcohol extract is taken and extracted with water under high temperature and high pressure to obtain a water-extraction mixture. A pharmaceutical composition for regulating blood sugar of a camphor extract, comprising: an effective amount of a camphor extract selected from any one of items 1 to 8 of the patent application. A method for preparing a citron extract, comprising the steps of: placing a fragrant cilantro leaf in an aqueous alcohol solution; extracting to obtain an alcohol extraction mixture; separating the alcohol extraction mixture to obtain an alcohol extract and an alcohol extraction And taking the alcohol extract, extracting with water under high temperature and high pressure to obtain a water extraction mixture. The method of claim 12, wherein the citron leaf is a fragrant leaf powder or a fragrant leaf fragment. The method of claim 12, wherein the alcohol-containing aqueous solution has an alcohol content percentage by volume of from 70% to 100%. The method of claim 12, further comprising: separating the water extraction mixture to obtain an aqueous extract. The method of claim 15, further comprising: mixing the alcohol extract and the water extract. The method of claim 16, wherein the alcohol extract and the aqueous extract are mixed in a ratio of from 3:1 to 6:1. The method of claim 15, further comprising: separately drying or concentrating the alcohol extract and the aqueous extract, and mixing. The method of claim 18, wherein the dried or concentrated alcohol extract and the dried or concentrated aqueous extract are in a ratio of from 3:1 to 6:1.