201117820 六、發明說明： 【發明所屬之技術領域】 本發明係關於一種香椿萃取物，特別係關於一種應用 於調節血糖的香椿萃取物。 【先前技術] 夕 】名椿或捲樹’學名為⑼幻\?)，是一 .基芥笙从t生落葉性喬本植物，原生於中國東南、西南至 !白=農現已全球性地廣泛栽植。香椿侧枝少，樹皮 極具利用價值特殊氣味，且其嫩葉可食用又終年可採集， 樹種===樹型優美’十分適於作為行道樹或觀賞 用於製作家具或建材。此外，民間自古 就有流傳「舍用夭社 5用日椿，不染雜病」的說法，而在傳統藥血 中記載亦有記載食用香椿或將香椿入藥的各項好處，包ς 清熱解毒、健胃理氣及止血固精等，都透露了香椿在醫藥 學上或保健食品上的發展潛力。另外，現代生物學的研究 亦藉由分子或細胞實驗上的證據指出香椿在應用於癌症 或腫瘤治療方面頗有療效，更加彰顯了栽植香椿在健康保 健方面可提供的實用功效與經濟利益。 除應用於特定癌症的治療外，傳統作法或習知知識 有食用或制香椿以達成降低錄的作法H 取飲用時僅是單純地㈣水沖泡㈣㈣或香椿茶 飲用。此種「香椿茶」不僅在香椿成分比例上難以定量^ 201117820 其對降低血=’乃至於調節或舒缓代謝症候群（例如糖尿 病癥狀發Ο及其後續所引發之病症的功效亦不顯著， 更其作用機制亦缺乏任何科學理論的支持。另外，此種習 知之香椿葉利用方式對香#中調節血糖之活性成分的萃 取亦十/刀有限，難以進一步調製為健康食品或醫藥組合 物。 因此，如何提供一種香椿萃取物，其可藉由特定的製 備方法萃取，以有效地取得香椿葉中的活性成分，並具有 # 良好的血糖調節功能，已成為重要課題之一。 【發明内容】 有鑑於上述課題，本發明之目的為提供一種香椿萃取 物’其玎藉由特定的製備方法萃取，並具有良好的血糖調 節功能。 本發明之另一目的則為提供一種製備香椿萃取物之 方法，其可用於製備上述之香椿萃取物。 為達上述目的’依據本發明之一種香椿萃取物，是經 由下列步驟所製備，包含將一香椿葉置於一含醇之水溶液 中；以及萃取以得一醇萃取混合物。 依據本發明之香椿萃取物，在其製備步驟中，香椿葉 是為香椿葉粉末或香椿葉碎片。在本發明之一實施例中， 香椿葉是為一香椿嫩葉粉末。 依據本發明之香椿萃取物，在其製備步驟中，含醇之 水溶液的醇含量體積百分比為70%至100%。在本發明之 201117820 一實施例中，含醇之水溶液的醇含量體積百分比為70%。 在本發明之一實施例中，本發明之香椿萃取物的製備 步驟更包含分離醇萃取混合物，以得一醇萃取液以及一醇 萃取物。 在本發明之一實施例中，本發明之香椿萃取物的製備 步驟更包含乾燥或濃縮處理醇萃取液。 · 在本發明之一實施例中，:本發明之香椿萃取物的製備 鳙步驟更包含取醇萃取物，以永於高溫高壓下萃取以得〆水 卒取混合物。 在本發明之一實施例中，本發明之香椿萃取物的製備 步驟更包含分離水萃取混合物，以得一水萃取液。 在本發明之一實施例中，本發明之香椿萃取物的製備 步驟更包含混合醇萃取液及水萃取液。 依據本發明之香椿萃取物，其製備步驟中的醇萃取液 及水萃取液之混合比例可為3:1至6:1。在本發明之一實施 例中，上述之醇萃取液及水萃取液之體積混合比例為3:1。 在本發明之一實施例中，本發明之香椿萃取物的製備 步驟更包含分別乾燥或濃縮上述之醇萃取液及水萃取 液’並加以混合。 ： ； 也依據本發明之香椿萃取杨，其製備步驟中的經乾燥或 遭縮的醇萃取液及經乾燥或濃縮的水萃取液之混合比例 3 · 1 $ & 6:1。在本發明之一實施例中’上述之經乾燥或濃 鈿的醇萃取液及經乾燥或濃縮的水萃取液之體積混合比 例為3:1.。 201117820 依據本發明之一種製備香椿萃取物之方法，包含將一 香椿葉置於一含醇之水溶液中；以及萃取以得一醇萃取混 合物。 依據本發明之一種香椿萃取物，其是應用於調節血 ' 糖，且經由下列步驟所製備，包含將一香椿葉置於一含醇 之水溶液中；以及萃取以得一醇萃取混合物。 承上所述，因依據本發明之一種香椿萃取物係以醇溶 液作為萃取溶劑，得以有效地取得香椿葉中的活性成分。 • 再者，利用本發明所揭露之製備步驟所得之香椿萃取物可 具有較佳之血糖調節能力，適於進一步應用在保健食品甚 至醫藥領域。 【實施方式】 以下將參照相關圖式，說明依據本發明之一種香椿萃 取物，其中相同的元件將以相同的參照符號加以說明。 _ 圖1.為本發明較佳實施例之一種香椿萃取物的製備步 驟流程圖，同時亦為本發明較佳實施例之一種製備香椿萃 取物之方法的步驟流程圖。請參考圖1所示，依據本發明 之一種香椿萃取物，是經由下列步驟所製備，包含將一香 椿葉置於一含醇之水溶液中（S10);以及萃取以得一醇萃 取混合物A1 ( S20)。 於步驟S10中，香椿葉是由香椿（學名為 )上採集下來的葉片，其型式可例如但不限於一香 捲葉粉末或一香捲葉碎片。在本實施例中，香椿葉是為一 201117820 香椿葉碎片，其是由香椿嫩葉經研磨、或禱碎、或其他可 達成相同目的之手段所得。因香椿葉碎片或粉末比完整的 香椿葉片具有較高的表面積與體積比，可有效提高萃取效 率。 * 在本實施例中，上述之含醇之水溶液中的醇類可例如 但不限於曱醇、或乙醇、或丙醇、或異丙醇、或正丁醇、 或異丁醇、或其任一組合；而含醇之水溶液較佳是為一含 $ 乙醇之水溶液。 另外，為有效萃取香椿葉中之活性成分，在本實施例 中，含醇之水溶液的醇含量佔總溶液的體積百分比為70% 至100% ;較佳地，醇含量佔總溶液的體積百分比為70%。 於含醇之水溶液中萃取香椿葉的方法在實施上並無 特別限制，主要是對位於含醇之水溶液中的香椿葉進行搖 動、或晃動、或震盪、或其他任何類似之手段，以達成獲 取含有香椿活性成分之醇萃取混合物A1之目的。需注意 Φ 的是，不論是搖動、或晃動、或震盪、或其他任何類似之 手段，皆可以利用手動、或半自動化、或自動化的方式進 行，在實施上並無特別的限制。在本實施例中，適於達成 上述目的之手段可例如但不限於以機器或儀器持續、或間 歇、或週期性地搖動或晃動裝有含醇之水溶液及香椿葉的 容器、或提供超音波於一段時間内對含醇之水溶液中的香 椿葉進行震盪。 因此，將香椿葉置於具有特定比例的含醇之水溶液 中，並加以萃取，可得醇萃取混合物A卜與習知直接以熱 201117820 水沖泡香椿的作法相較，依據本發明所得之醇萃取混合物 A1具有較高之香椿活性成分及較佳之血糖調節能力（進一 .步說明請參照實驗例2 )。 凊參考圖1所示，在本實施例中，於萃取以得醇萃取 混合物A1之步驟S20後，更包含一分離醇萃取混合物 A1 ’以得一醇萃取液ΑΠ以及一醇萃取物A12的步驟 (S30)°於此步驟S3〇中，分離醇萃取混合物A1之方法 可例如但不限於將醇萃取混合物Ai進行離心，使醇萃取 # 物A12沈殿而與醇萃取液All分離；或是利用過據的方 式，來分離醇萃取物A12和醇萃取液All。在本實施例中， 係利用離心處理來分離醇萃取物A12和醇萃取液All，醇 萃取液All為上清液，而醇萃取物A12為沈澱於容器底部 或側壁之殘渣。另外，離心所使用之轉速及時間長短則可 視實際實施情況進行調整，並無特別限制。 請參考圖1所示，在本實施例中，於得到醇萃取液A11 以及醇萃取物A12之步驟S30後，更包含一取醇萃取物 ^ A12,以水於高溫高壓下萃取以得一水萃取混合物H1之步 驟（S40)。詳細而言，在此步驟中，較佳是取醇萃取物， 以水於溫度121°C且壓力為1 kgw/cm2之環境下作用10呈 30分鐘萃取以得一水萃取混合物H1。 請參考圖1所示，在本實施例中，於以水於高溫高壓 下萃取以得水萃取混合物H1的步驟S40後，更包含一分 離水萃取混合物H1，以得一水萃取液HU之步驟（S50) ° 於步驟S50中，分離水萃取混合物H1所使用之方法、及/ i 9 201117820 S3〇中之醇萃取品合 或設定條件、及/或器械皆可參考步騍 物μ的分離，於此不再贅的是，實施上仍亦 注意醇及水之間的差異而再進行適當調整201117820 VI. Description of the Invention: TECHNICAL FIELD OF THE INVENTION The present invention relates to a citron extract, and more particularly to a citron extract for use in regulating blood sugar. [Prior Art] Xi's name or volume tree 'scientific name (9) illusion \?), is a basal mustard from the t-deciduous hormonal plant, native to southeast China, southwest to white! Sexually widely planted. There are few side branches of the camphor, and the bark is very valuable for use, and its young leaves are edible and can be collected all year round. The tree species === beautiful tree shape is very suitable for use as a street tree or for making furniture or building materials. In addition, since ancient times, there has been a saying in the community that "the use of 夭 5 5 椿 椿 椿 椿 不 不 不 不 不 不 不 不 不 不 不 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统 传统, stomach and qi, and hemostasis, etc., all reveal the development potential of camphor in medicine or health food. In addition, modern biological research has also shown through molecular or cellular experimental evidence that the use of camphor in cancer or tumor treatment is more effective, and it further demonstrates the practical and economic benefits that can be provided in the health and wellness of planted camphor. In addition to the treatments used in specific cancers, traditional practices or customary knowledge are used to eat or make citron in order to achieve a reduced recording. H When drinking, simply drink (iv) water (4) (4) or citron tea. This kind of "Cinnamon Tea" is not only difficult to quantify in the proportion of toon ingredients. 201117820 It is not effective in reducing blood = 'but even regulating or soothing metabolic syndrome (such as the symptoms of diabetes and its subsequent symptoms). The mechanism of action is also lacking in any scientific theory. In addition, the use of this known method of using citron leaves is limited to the extraction of active ingredients for regulating blood sugar in Xiang#, which is difficult to further prepare into a health food or pharmaceutical composition. How to provide a citron extract which can be extracted by a specific preparation method to effectively obtain an active ingredient in citron leaves, and has a good blood sugar regulating function, and has become one of important subjects. In view of the above problems, an object of the present invention is to provide a citron extract which is extracted by a specific preparation method and which has a good blood sugar regulating function. Another object of the present invention is to provide a method for preparing a citron extract. It can be used to prepare the above-mentioned citron extract. For the above purpose, a kind according to the invention The camphor extract is prepared by the following steps, comprising: placing a vanilla leaf in an alcohol-containing aqueous solution; and extracting to obtain an alcohol extract mixture. According to the present invention, the camphor extract, in the preparation step thereof, the camphor leaf It is a fragrant leaf powder or a fragrant leaf fragment. In one embodiment of the present invention, the citron leaf is a fragrant green leaf powder. The citron extract according to the present invention, the alcohol content volume of the alcohol-containing aqueous solution in the preparation step thereof The percentage is from 70% to 100%. In an embodiment of 201117820 of the present invention, the volume percentage of the alcohol content of the alcohol-containing aqueous solution is 70%. In one embodiment of the present invention, the preparation step of the toon extract of the present invention is further The separation alcohol extraction mixture is included to obtain an alcohol extract and an alcohol extract. In one embodiment of the present invention, the preparation step of the toon extract of the present invention further comprises drying or concentrating the alcohol extract. In one embodiment, the preparation step of the toon extract of the present invention further comprises extracting an alcohol extract, and extracting it for a long time under high temperature and high pressure to obtain a mixture of water and water. In one embodiment of the present invention, the step of preparing the camphor extract of the present invention further comprises separating the water extract mixture to obtain an aqueous extract. In one embodiment of the present invention, the camphor extract of the present invention The preparation step further comprises mixing the alcohol extract and the water extract. According to the present invention, the mixing ratio of the alcohol extract and the water extract in the preparation step may be from 3:1 to 6:1. In one embodiment, the volume ratio of the above-mentioned alcohol extract and water extract is 3: 1. In one embodiment of the present invention, the preparation step of the camphor extract of the present invention further comprises separately drying or concentrating the above. The alcohol extract and the water extract are 'mixed together. : . . . The extract of the dried or condensed alcohol extract and the dried or concentrated water extract in the preparation step is also extracted according to the present invention. · 1 $ & 6:1. In one embodiment of the invention, the volume mixing ratio of the above dried or concentrated alcohol extract and the dried or concentrated aqueous extract is 3:1. 201117820 A method of preparing a citron extract according to the present invention, comprising: placing a citron leaf in an aqueous alcoholic solution; and extracting to obtain an alcohol extraction mixture. A citron extract according to the present invention is for use in modulating blood sugar, and is prepared by the following steps, comprising: placing a fragrant citron leaf in an aqueous alcoholic solution; and extracting to obtain an alcohol extraction mixture. As described above, since the camphor extract according to the present invention uses an alcohol solution as an extraction solvent, the active ingredient in the leaves of the camphor tree can be efficiently obtained. Further, the citron extract obtained by the preparation step disclosed in the present invention can have a better blood sugar regulating ability and is suitable for further application in health foods and even medicine. [Embodiment] Hereinafter, a citron extract according to the present invention will be described with reference to the related drawings, wherein the same elements will be described with the same reference numerals. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a flow chart showing the preparation of a citron extract according to a preferred embodiment of the present invention, and is also a flow chart of the method for preparing a citron extract in accordance with a preferred embodiment of the present invention. Referring to FIG. 1, a citron extract according to the present invention is prepared by the following steps, comprising: placing a fragrant cilantro leaf in an aqueous alcohol solution (S10); and extracting to obtain an alcohol extraction mixture A1 ( S20). In step S10, the camphor leaves are leaves collected from the camphor (scientific name), and the pattern may be, for example but not limited to, a scented leaf powder or a scented leaf fragment. In this embodiment, the citron leaves are a 201117820 fragrant shard leaf shard which is obtained by grinding, or praying, or other means for achieving the same purpose. Because the fragrant leaf fragments or powder have a higher surface area to volume ratio than the intact citron leaves, the extraction efficiency can be effectively improved. * In this embodiment, the alcohol in the above alcohol-containing aqueous solution may be, for example but not limited to, decyl alcohol, or ethanol, or propanol, or isopropanol, or n-butanol, or isobutanol, or any A combination; and the aqueous alcohol-containing solution is preferably an aqueous solution containing $ ethanol. In addition, in order to effectively extract the active ingredient in the citron leaves, in the present embodiment, the alcohol content of the alcohol-containing aqueous solution accounts for 70% to 100% by volume of the total solution; preferably, the alcohol content accounts for the volume percentage of the total solution. It is 70%. The method for extracting the citron leaves in the alcohol-containing aqueous solution is not particularly limited in practice, and is mainly for shaking, shaking, or oscillating, or any other similar means for the citron leaves in the aqueous solution containing alcohol to achieve the acquisition. The purpose of the alcohol extraction mixture A1 containing the citron active ingredient. It should be noted that Φ, whether it is shaking, shaking, or oscillating, or any other similar means, can be performed manually, or semi-automatically, or automatically, and there is no particular limitation on the implementation. In the present embodiment, means suitable for achieving the above object may be, for example but not limited to, continuously or intermittently or periodically shaking or shaking a container containing an aqueous solution containing alcohol and citron leaves, or providing ultrasonic waves by machine or instrument. The citron leaves in the aqueous solution containing alcohol are oscillated over a period of time. Therefore, the citron leaves are placed in a specific ratio of the alcohol-containing aqueous solution and extracted, and the alcohol extraction mixture A can be obtained. Compared with the conventional method of directly brewing the citron with hot 201117820 water, the alcohol obtained according to the present invention is compared. The extraction mixture A1 has a higher aroma active ingredient and a better blood sugar regulating ability (for the first step, please refer to Experimental Example 2). Referring to FIG. 1 , in the present embodiment, after the step S20 of extracting the alcohol extraction mixture A1, the step of further separating the alcohol extraction mixture A1 ′ to obtain an alcohol extract hydrazine and an alcohol extract A12 is further included. (S30) ° In this step S3, the method of separating the alcohol extraction mixture A1 may be, for example, but not limited to, centrifuging the alcohol extraction mixture Ai to separate the alcohol extraction from the alcohol extract All; or According to the manner, the alcohol extract A12 and the alcohol extract All are separated. In the present embodiment, the alcohol extract A12 and the alcohol extract All are separated by centrifugation, the alcohol extract All is the supernatant, and the alcohol extract A12 is the residue deposited on the bottom or side wall of the vessel. In addition, the number of revolutions and the length of time used for centrifugation can be adjusted depending on the actual implementation, and is not particularly limited. Referring to FIG. 1 , in the present embodiment, after the step S30 of obtaining the alcohol extract A11 and the alcohol extract A12, an alcohol extract A 12 is further taken, and the water is extracted under high temperature and high pressure to obtain a water. The step of extracting the mixture H1 (S40). Specifically, in this step, it is preferred to take an alcohol extract, extract it with water at a temperature of 121 ° C and a pressure of 1 kgw / cm 2 for 30 minutes to obtain a water-extraction mixture H1. Referring to FIG. 1 , in the embodiment, after the step S40 of extracting the mixture H1 with water under high temperature and high pressure, the step of extracting the water mixture H1 is further included to obtain the water extract HU. (S50) ° In step S50, the method used to separate the water extraction mixture H1, and the alcohol extraction product or setting conditions in the / i 9 201117820 S3〇, and/or the device can be referred to the separation of the step μ. What is no longer embarrassing here is that the implementation still pays attention to the difference between alcohol and water and then makes appropriate adjustments.
請參考圖！所示，在本實施例中，於得到水萃取液HU 的步驟S50後，更包含-分別乾燥或濃％醇萃取液An及 水萃取液H11，並加以混合之步驟（S6〇)。藉由步驟s6〇Please refer to the picture! As shown in the present embodiment, after the step S50 of obtaining the aqueous extract HU, the step (S6〇) of separately drying or enriching the alcohol extract An and the water extract H11, respectively, and mixing them is carried out. By step s6〇
處理，可得一經乾燥或濃縮的醇萃取液及一經乾燥或濃縮 的水萃取液。於步驟S60中，乾燥或濃縮^理醇萃取液 All及水萃取液H11之方法可例如但不限於蒸發處理 (evaporation)、或冷凍乾燥處理（lyophiHzaiion)、或喷 霧乾燥處理（spray-drying)、加熱濃縮、或其任一組合|<3 在本實施例中，是以冷凍乾燥處理醇萃取液All及水萃取 液H11。然而，需特別強調的是，對醇萃取液All及水萃 取液Η11之乾燥或濃縮處理並非一定要完全乾燥或將所含 之液體完全去除，可視實際需要有所調整’就意義上雨 言’乃是在將萃取液中的液體比例於此固液相混合物中所 佔之整體比例降低。換言之，·經乾燥或濃縮的醇萃取液及 經乾燥或濃縮的水萃取液可以不同形式存在’例如乾燥之 粉末或結晶、或未完全乾燥之粉末、或稍狀液體、或濃縮 之醇萃取液All及水萃取液H11 ° 此外，為使本發明之香椿萃取物具有更好的作用活 性，於步驟S60中’經乾燥或濃縮的醇萃取液及經乾燥或 濃縮的水萃取液的混合比例可為3:1至6:1。.在本實施例 中，混合比例為3:1。經混合後可得一具有較佳之香椿活 201117820 性成分利用1 力效之混合的香椿萃取物M。 m土 1再_人強§周的是’雖經由上述步驟S1G至S60 非發明之香椿萃取物（進-步說明請參照實驗 本發明之香椿萃取物必得經過此些步驟 才得以完成。反之，D +匕她 /、要在步驟S10及步驟S20執行後，Treatment may result in a dried or concentrated alcoholic extract and a dried or concentrated aqueous extract. In step S60, the method of drying or concentrating the alcohol extract All and the water extract H11 may be, for example but not limited to, evaporation, or freeze drying (lyophiHzaiion), or spray-drying. And heating and concentration, or any combination thereof|<3 In this embodiment, the alcohol extract All and the water extract H11 are treated by freeze-drying. However, it is important to emphasize that the drying or concentration treatment of the alcohol extract All and the water extract Η11 does not have to be completely dried or the liquid contained therein is completely removed, which may be adjusted according to actual needs. This is because the proportion of the liquid in the extract is reduced in the overall proportion of the solid-liquid mixture. In other words, the dried or concentrated alcoholic extract and the dried or concentrated aqueous extract may be present in different forms, such as a dry powder or crystallization, or an incompletely dried powder, or a slightly liquid, or a concentrated alcoholic extract. All and the water extract H11 ° In addition, in order to make the toon extract of the present invention have a better action activity, the mixing ratio of the dried or concentrated alcohol extract and the dried or concentrated water extract may be in step S60. It is 3:1 to 6:1. In this embodiment, the mixing ratio is 3:1. After mixing, a scented extract M which has a better blend of the scent of the scent. m soil 1 again _ human strong § week is 'although the non-invented citron extract through the above steps S1G to S60 (for the step-by-step description, please refer to the experiment of the present invention, the citron extract must be completed through these steps. Conversely, D +匕 her/, after execution in step S10 and step S20,
=自，明較佳實施例所揭示之步驟咖至步驟_進行 、=兀成後、或取自步驟咖至步驟S6G進行中或完成後 亚再加以-紐個㈣處理所狀萃取液、或混合液、或 =合物或其任-組合’ Μ蓋於本發明所揭示之香捲萃取 物的範圍内。以下將例舉以茲說明。 請參考圖1所示，在本實施例之另-態樣中，於得到 辱萃取液AU以及醇萃取物Α12之步驟謂後，可更包含 —乾燥或濃縮處理醇萃取液All的步驟（S41)。於步驟 S41中，乾$呆或濃縮處理的方式是與步驟S6〇中所述之乾 紐或濃縮處理方式相同，於此不再贅述。經此步驟S4l後 同樣可得之經乾燥或濃縮的醇萃取液。然而，其雖與步驟 S60中所得之經乾烯或濃縮的醇萃取液相同，但卻不需與 其他萃取物或萃取液混合，而同樣具有較習知熱水沖泡的 香椿茶為佳之活性功效。 同樣地，請參考圖1所示，在本實施例之又一態樣中， 於得到水萃取液ΗΠ的步驟·S5〇後，更包含一混合醇萃取 液All及水萃取液H11之步驟（s61)。經步驟S61處理後 可得一混合的萃取液’其亦為依據本發明所得之香椿萃取 物的一種態樣，益同樣具有較習知熱水沖泡的香椿茶為佳 201117820 之活性功效。當然，若為提高混合的萃取液中之香椿萃取 物的活性功效，在本實施例中，醇萃取液All及水萃取液 H11之混合比例可為3:1至6.:1，而較佳的混合比例為3:1。 細上所述，因依據本發明之一種香椿萃取物係以醇溶 液作為萃取溶劑，以有效地取得香椿葉中的活性成分。再 者，利用本發明所揭露之製備步驟所得之香椿萃取物可具 有車父佳之血糖調節能力，適於進一步應用在保健食品甚至 籲醫藥領域。另外，於實施例中，在萃取後按照特定比例將 所得的產物混合，還可增加香椿萃取物中活性成分之效 果。 本發明亦揭露之一種香椿萃取物，係應用於調節血 糖，且此香椿萃取物是經由下列步驟所製備，包含將一香 椿葉置於一含醇之水溶液中；以及萃取以得一醇萃取混合 物。 此外，本發明又揭露一種香椿萃取物，應用於增加過 鲁氧化小體增殖激活受體-T ( peroxisome proliferators activated receptor-gamma，PPAR-γ )之活性，且此香椿萃取 物是經由下列步驟所製備，包含將一香椿葉置於一含醇之 水溶液中；以及萃取以得一醇萃取混合物。由於上述兩種 香椿萃取物之製備方法中所使用的手段、及/或方式、及/ 或溶液、及/或器械皆與前述所例舉之實施例中說明的手 段、及/或方式、及/或溶液、·及/或器械相同，.於此不再贅 t ijji 〇 * ·· 在習知技術中，已知過氧化小體增殖激活受體_τ的 201117820 活化具有可增強細胞對胰島素的敏感度之功效，並可藉此 機轉降低血糖濃度。而本發明所揭露之香椿萃取物則證實 具有提高過氡化小體增殖激活受體-τ活性之能力（進一 步說明請參照實驗例2)，因此，本發明之香椿萃取物具有 調製成為保健食品或飲品之潛力，並可依據所選用之萃取 物態樣，例如乾燥粉末或萃取液，以本發明領域中之習知 技術依據所需對萃取物進行固體或液體的調製或加工。另 外，還可選擇性地包含適當的添加物/劑、及/或調味劑、 鲁 及/或緩衝劑、及/或安定劑、及/或其他類似物，進而決定 保健食品或飲品在香椿萃取物活性功效外的其他特徵。據 此所得之保健食品或飲品，不僅具有較習知之香椿茶為佳 的活性功效，更利於商品化及推廣使用。舉例來說，在本 發明之一非限制性實施例中，在將本發明之香椿萃取物調 製成為一口服液體狀製品時，所選用之安定劑為黃耆膠 (tragacanth)、及/或阿拉伯膠（gumarabic)及/或明膠， $ 至於選用之缓衝劑為檸檬酸鈉。 Φ 本發明另揭露一種用於調節血糖之香椿萃取物之醫 藥組合物，包含一有效量之香捲萃取物，其是選自於上述 實施例之各態樣中之任一香椿萃取物。此外，所述之醫藥 組合物可更包含一醫藥學上可接受之載劑。其中，所述之 「載劑」一詞包含任何本發明技術領域中習知的載劑，而 可例如但不限於水、或生理食鹽水、或甘油、或有機溶劑、 或安定劑、或螯合劑、或稀釋劑、或防腐劑、或乳化劑、 或懸浮劑、或凝膠劑、或脂質體等。另外，在本發明之一 13 201117820 實施例中，所述之醫藥組合物更包含一賦形劑。據此，此 醫藥組合物得以製備成為一口服固體狀製劑，其類型包括 鍵劑、或經包覆的鼓劑、或顆粒、或粉末、或膠囊、或其 他相似者。 再者，除了適合於口服投予之外，本發明所揭露之醫 藥組合物亦可依據本發明領域中之習知技術製備成為透 過非經腸胃道、或注射、或局部使用等途徑進行投予之醫 • 藥組合物。 以下將列舉數個實驗例以說明依本發明較佳實施例 之香椿萃取物的製備步驟及其提高過氧化小體增殖激活 受體-7活性之功效。 實驗例1.香椿萃取物之製備步驟 下述將以一實驗例說明本發明之香椿萃取物之一種 製備步驟。 取一公斤的香椿嫩葉碎片浸泡於10公升的70%乙醇 • 溶液中，再於室溫下以150rpm震盪萃取香椿嫩葉碎片10 小時，以得醇萃取混合物A1 k以10,000 Xg離心醇萃取混 合物A1約20分鐘後，可將醇萃取液All (上清液）及醇 萃取物A12 (香椿嫩葉殘渣）’分離。將離心分離後之醇萃 取物A12再加入4公升的水，於溫度121°C且壓力為1 kgw/cm2之環境下萃取20分鐘，可得水萃取混合物H1， 再以10,000 Xg離心水萃取混合物H1約20分鐘，而藉此 分離出水萃取液H11 (上清液）。然後，分別對醇萃取液 All及水萃取液H11進行冷凍乾燥處理，得到經乾燥的醇 201117820 萃取液及經乾燥的水萃取液。最後，將上述之經乾燥的醇 萃取液及經乾燥的水萃取液依照3:1的比例混合而成為混 合的香椿萃取物Ml。 實驗例2.香椿萃取物提高過氧化小體增殖激活受體活 性之試驗 (一）細胞處理 預先於12孔培養皿（l2_well plate)中依細胞培養之 習知技術培養人類肝癌細胞株（HepaG2細胞）。其後，每 . 孔中置入溶液A及溶液B。其中，溶液A是為含有〇4 # g 載體 pG51uc 及 0.2 載體 pBPPARr 之 175 //1 不含 胎牛血清之伊格爾式基本成分培養基（FBSfreeDMEM)， 而/谷液B則疋為含有4 脂質體2000 (】ip0fectamine 2000)之ι75 不含胎牛血清之伊格爾式基本成分培養 基。將溶液A及溶液β混合後靜置40分鐘（溶液a及溶 液B在混合前是採個別配製之方式混合後之溶液a及 φ /谷液B ’分別加入位於12孔培養皿中的細胞，並持續培養 24小時，再依習知方式將培養基更換為正常之伊格爾式基 本成分培養棊，並再培養24小時。然後，分別在培養皿 中的不同孔内加入皮利目同溶液（pioglitazone，一種藥物， 可作為過氧化小體增殖激活受體-7的結合物，具有活化 過氧化小體增殖激活受體之功效，可促進體内血糖代 謝作用，從而降低血糖濃度）、或經熱水沖泡之香椿茶乾 燥物（其是先以1〇〇它的熱水沖泡香椿茶30分鐘後，離心 取上清液，再經冷凍乾燥所得者）、或經乾燥或濃縮的醇 15 201117820 萃取液之溶液、或經乾燥或濃縮的水萃取液之溶液、或混 合的香椿萃取物Ml之溶液:，待於細胞培養箱内培養24 小時後進行冷光分析。 . (二） 冷光分析（Luminescence analysis) 由於轉染（transfect)細胞所用之載體是以表現螢火 蟲螢光素酶之基因作為報導基因（reporter gene)，並其表 現量受到過氧化小體增殖激活受體-γ活性調控。因此， •利用本發明領域中之習知技術分析螢火蟲螢光素酶 (firefly luciferase )的表現量可判定細胞内過氧化小體增 殖激活受體-7之活性強弱。 據此，分別於培養皿的各孔内加入250 的細胞溶 解缓衝液（cell lysis buffer)。待細胞充分溶解後，分別在 各孔中取出10 #1的細胞溶解液加入螢火蟲螢光素酶基質 (firefly luciferase substrate)中。混合並待反應一段時間 後，利用Mini-Lumat LB 9506測定儀偵測螢火蟲螢光素酶 鲁作用後所表現之冷光值；之後，再加入水母螢光素酶基質 (Renilla luciferase substrate )偵測水母螢光素酶作用後所 表現之冷光值。最後，以螢火蟲螢光素酶作用後所表現之 冷光測定值除以水母營光素酶作用後所表現之冷光測定 值與空白控制組（未加入任何藥劑、或萃取液、或萃取物） 之冷光測定值及正控制組（加入皮利_達到濃度為〇. 1 从g/ml)之冷光測定值進行比:較’即可得知各香捲萃取液 或萃取物活化過氧化小體增殖激活受體_r的強弱差異。 (三） 結果分析 ； 16 201117820 將上述加入經熱水沖泡之香椿茶乾燥物'及經乾燥或 濃縮的醇萃取液之溶液、及經乾燥或濃縮的水萃取液之溶 液、及混合的香椿萃取物Ml之溶液之各組分別進行與過 氧化小體增殖激活受體-T之活性有關的冷光測試。 圖2是為以不同濃度之混合的香椿萃取物Ml之溶液 處理後所表現的冷光強度’圖3是為以不同漠度之經乾燥 或濃縮的水萃取液之溶液處理後所表現的冷光強度，圖4 是為以不同濃度之經乾燥或濃縮的醇萃取液之溶液處理=, from the steps disclosed in the preferred embodiment, to the step _ proceed, = after the formation, or from the step coffee to the step S6G in progress or after the completion of the sub-reaction - New (four) treatment of the extract, or The mixed solution, or the compound or any combination thereof, is covered within the scope of the fragrant extract disclosed herein. The following will be exemplified. Referring to FIG. 1 , in another aspect of the embodiment, after the step of obtaining the aspirating extract AU and the alcohol extract Α12, the step of drying or concentrating the alcohol extract All may be further included (S41). ). In the step S41, the manner of the dry or staying process is the same as the method of the dry or concentrated process described in the step S6, and will not be described again. The dried or concentrated alcohol extract is also obtained after this step S4l. However, although it is the same as the dry olefin or concentrated alcohol extract obtained in the step S60, it does not need to be mixed with other extracts or extracts, and the citron tea which is similar to the conventional hot water brewing is preferably active. efficacy. Similarly, referring to FIG. 1 , in another aspect of the embodiment, after the step S5 of obtaining the water extract enthalpy, the step of mixing the alcohol extract All and the water extract H11 is further included ( S61). After the treatment in the step S61, a mixed extract is obtained, which is also a kind of the extract of the camphor extract obtained according to the present invention, and the same has the activity of the hot tea brewed with the known hot water. Of course, in order to increase the activity of the extract of the camphor extract in the mixed extract, in this embodiment, the mixing ratio of the alcohol extract All and the water extract H11 may be from 3:1 to 6.:1, and preferably. The mixing ratio is 3:1. As described above, a citron extract according to the present invention is an alcohol solution as an extraction solvent to effectively obtain an active ingredient in citron leaves. Further, the citron extract obtained by the preparation step disclosed in the present invention can have the blood sugar regulating ability of the car father, and is suitable for further application in the health food or even the medical field. Further, in the examples, the obtained product may be mixed in a specific ratio after the extraction, and the effect of the active ingredient in the camphor extract may also be increased. The present invention also discloses a citron extract for regulating blood sugar, and the citron extract is prepared by the following steps, comprising: placing a citron leaf in an aqueous solution containing alcohol; and extracting to obtain an alcohol extraction mixture. . In addition, the present invention further discloses a citron extract which is used for increasing the activity of peroxisome proliferators activated receptor-gamma (PPAR-γ), and the citron extract is subjected to the following steps. The preparation comprises: placing a citron leaf in an aqueous solution containing alcohol; and extracting to obtain an alcohol extraction mixture. The means, and/or manner, and/or the solution, and/or the apparatus used in the preparation method of the above two kinds of toona sinensis extracts are the same as those described in the above-exemplified examples, and/or / or the solution, · and / or the same instrument, this is no longer 赘t ijji 〇 * · · In the prior art, it is known that the peroxisome proliferator-activated receptor _τ 201117820 activation has enhanced cell-to-insulin The sensitivity of the effect, and can be used to reduce blood sugar levels. However, the extract of the camphor extract disclosed in the present invention has the ability to enhance the activity of the overactive body to activate the receptor-τ activity (for further explanation, refer to Experimental Example 2), and therefore, the camphor extract of the present invention has a modulation into a health food. Or the potential of the beverage, and depending on the selected extract form, such as a dry powder or extract, the extract may be solid or liquidly prepared or processed in accordance with conventional techniques in the art. In addition, optionally, suitable additives/agents, and/or flavoring agents, agents and/or buffers, and/or stabilizers, and/or the like may be optionally included to determine the health food or drink in the toon extract. Other characteristics than the activity of the substance. According to this, the health food or drink obtained has better activity than the well-known fragrant tea, and is more conducive to commercialization and popularization. For example, in one non-limiting embodiment of the invention, when the citron extract of the present invention is formulated into an oral liquid preparation, the stabilizer used is tragacanth, and/or arabic. Gumarabic and / or gelatin, $ As for the buffer used is sodium citrate. Φ The present invention further discloses a pharmaceutical composition for regulating blood sugar of a camphor extract comprising an effective amount of a fragrant extract selected from any of the various embodiments of the above embodiments. Furthermore, the pharmaceutical composition may further comprise a pharmaceutically acceptable carrier. Wherein the term "carrier" includes any carrier known in the art, and may be, for example but not limited to, water, or physiological saline, or glycerin, or an organic solvent, or a stabilizer, or a chelate. Mixtures, or diluents, or preservatives, or emulsifiers, or suspensions, or gels, or liposomes, and the like. Further, in one embodiment of the present invention, 13 201117820, the pharmaceutical composition further comprises an excipient. Accordingly, the pharmaceutical composition is prepared as an oral solid preparation of a type comprising a key, or a coated drum, or a granule, or a powder, or a capsule, or the like. Furthermore, in addition to being suitable for oral administration, the pharmaceutical compositions disclosed herein can also be prepared by parenteral, injection or topical administration according to conventional techniques in the art of the present invention. Medical • Pharmaceutical composition. Several experimental examples will be enumerated below to illustrate the preparation steps of the camphor extract according to the preferred embodiment of the present invention and the effect of increasing the activity of the peroxisome proliferator-activated receptor-7. Experimental Example 1. Preparation procedure of Toona sinensis extract A preparation step of the toon extract of the present invention will be described below by way of an experimental example. One kilogram of the leaves of the fragrant green leaves were soaked in 10 liters of 70% ethanol solution, and then the leaves of the fragrant leaves were extracted at 150 rpm for 10 hours at room temperature to obtain an alcohol extraction mixture A1 k to extract the mixture A1 at 10,000 Xg. After 20 minutes, the alcohol extract All (supernatant) and the alcohol extract A12 (fragrant green leaf residue) were separated. The centrifuged alcohol extract A12 was further added to 4 liters of water, and extracted at a temperature of 121 ° C and a pressure of 1 kgw / cm 2 for 20 minutes to obtain a water extraction mixture H1, and then the mixture was extracted with 10,000 X g of centrifugal water. H1 was about 20 minutes, and the aqueous extract H11 (supernatant) was separated therefrom. Then, the alcohol extract All and the water extract H11 were freeze-dried to obtain a dried alcohol 201117820 extract and a dried water extract. Finally, the above dried alcohol extract and the dried aqueous extract were mixed in a ratio of 3:1 to become a mixed camphor extract M1. Experimental Example 2. Test for Enhancing Peroxisome Proliferation-Activated Receptor Activity by Toona sinensis Extract (I) Cell Treatment Human hepatoma cell line (HepaG2 cells) was cultured in a well-known technique of cell culture in a 12-well culture dish (12-well plate). ). Thereafter, solution A and solution B were placed in each well. Among them, solution A is 175 //1 containing the 〇4 #g vector pG51uc and 0.2 carrier pBPPARr, which is contained in the egg-shaped basic component medium (FBSfreeDMEM) without fetal bovine serum, and / glutamate B is contained in 4 lipids. ι 75 of the body 2000 (] ip0fectamine 2000) contains the Eagle's basic ingredient medium of fetal bovine serum. The solution A and the solution β were mixed and allowed to stand for 40 minutes (the solution a and the solution B were separately mixed and the solutions a and φ / the solution B were added to the cells in the 12-well culture dish, respectively). The culture was continued for 24 hours, and the medium was changed to the normal Eagle-like basic ingredient culture medium in a conventional manner, and cultured for another 24 hours. Then, the solution was added to the different wells in the culture dish. Pioglitazone, a drug that acts as a conjugate of peroxisome proliferator-activated receptor-7, has the effect of activating peroxisome proliferator-activated receptors, promotes blood glucose metabolism in the body, thereby lowering blood glucose levels, or Hot water brewed citron tea dry matter (which is obtained by first brewing the citron tea with 1 Torr of hot water for 30 minutes, centrifuging the supernatant, and then freeze-drying), or drying or concentrating the alcohol 15 201117820 A solution of the extract, or a solution of the dried or concentrated aqueous extract, or a solution of the mixed camphor extract M1: after 24 hours of incubation in a cell culture incubator for cold light analysis. (2) Cold Luminescence analysis The vector used for transfecting cells is a reporter gene that expresses firefly luciferase, and its expression is activated by peroxisome proliferator-activated receptor-γ activity. Therefore, the expression of firefly luciferase can be determined by the conventional techniques in the field of the present invention to determine the activity of intracellular peroxisome proliferator-activated receptor-7. Add 250 cell lysis buffer to each well of the culture dish. After the cells are fully dissolved, remove the 10 #1 cell lysate into each well and add the firefly luciferase substrate. Mixing and reacting for a period of time, using the Mini-Lumat LB 9506 meter to detect the luminescence value of the firefly luciferase action; then adding the serotonin luciferase substrate The luminescence value measured by the action of the jellyfish luciferase. Finally, the luminescence measured by the firefly luciferase The cold light measurement value and the positive control group (added to the Pili_Achieve concentration) 1 From the measured value of the cold light of g/ml): the difference between the strength of each of the extracts or the extract activated peroxidase-activated receptor _r can be known. (3) Analysis of results; 201117820 Adding the above solution of the hot tea brewed citron tea dried product and the dried or concentrated alcohol extract solution, and the dried or concentrated water extract solution, and the mixed citron extract M1 solution The group performed a luminescence test related to the activity of the peroxisome proliferator-activated receptor-T, respectively. Figure 2 is the luminescence intensity exhibited after treatment with a solution of mixed concentrations of Toona sinensis extract M1 at different concentrations. Figure 3 is the luminescence intensity exhibited after treatment with a solution of dried or concentrated aqueous extracts of different indifference. Figure 4 is a solution of different concentrations of dried or concentrated alcohol extract
後所表現的冷光強度 一-▼.，.…% 7JC 沖泡之香椿茶乾燥物處理後所表現的冷光強度。請同昧參_ 考圖2至圖5，顯然地，依據本發明之香椿萃取物，不认 是混合的香椿萃取物Ml (圖2)、或經乾燥或濃縮的醇^ 取液（圖3)、或經乾燥或濃縮的水萃取液（圖4)皆具有 較熱水沖泡之香椿茶乾燥物（圖5)為佳的過氧化小體择 殖激活受體-r活化功效。若個別檢視之，圖2中混人^ 香椿萃取物Ml係指經乾燥的醇萃取液及經乾燥的水萃取 液依照3:1的比例混合而成，其活化過氧化小體增殖激、、 文體-7"的功效最佳，約相當於正控制組的11〇%。圖3 經乾燥或濃縮的水萃取液隨著劑量的提升而活化過& 小體增殖激活受體’最高約相當於絲制組（加乳 二μ之皮利酮）之桃以上的致果。❿圖4中經 曲1 縮的醇萃取液活化過氧化小體增殖激活受體1之功= 又較經乾燥或濃縮的水萃取液為佳，^目 ^則 6。%的效果。如圖5所示，至於習知以熱水沖泡= Γ ^ 1 201117820 乾燥物，則僅相當於正控制組之約40%左右的活化效果。 值得一提的是，圖2中混合後的香椿萃取物Ml則具有分 別比經乾燥或濃縮的醇萃取液（圖3)及經乾燥或濃縮的 水萃取液（圖4 )較佳的效果。 以上所述僅為舉例性，而非為限制性者。任何未脫離 本發明之精神與範疇，而對其進行之等效修改或變更，均 應包含於後府之申請專利範圍中。 【圖式簡單說明】 圖1係本發明較佳實施例之一種香椿萃取物的製備步 驟流程圖；以及 圖2至圖5係以不同劑量之本發明之各香椿萃取物及 習知之香椿茶乾燥物處理後所表現的冷光強度數據圖。 【主要元件符號說明】 • A1 :醇萃取混合物 All :醇萃取液 A12 :醇萃取物 H1 :水萃取混合物 H11 :水萃取液 ：The intensity of the cold light after the one-▼.,....% 7JC The luminescence intensity of the brewed tea after drying. Please refer to Fig. 2 to Fig. 5, obviously, the camphor extract according to the present invention is not considered to be a mixed camphor extract M1 (Fig. 2), or a dried or concentrated alcohol extract (Fig. 3). ), or the dried or concentrated water extract (Fig. 4) has a better effect on the activation of the peroxisome-activated receptor-r than the dried tea (Fig. 5). If examined individually, the mixed extract of M. sinensis in Figure 2 means that the dried alcohol extract and the dried water extract are mixed in a ratio of 3:1, and the activated peroxidized body is proliferated, Stylistic -7" works best, about 11% of the positive control group. Figure 3 The dried or concentrated aqueous extract is activated with increasing dose & the small body proliferator-activated receptor' is about the highest value of the peach-like group (with the milk of the second μ pirinone). . The activity of the peroxisome proliferator-activated receptor 1 activated by the transesterified alcohol extract in Fig. 4 is better than that of the dried or concentrated aqueous extract. %Effect. As shown in Fig. 5, as for the dry matter of hot water brewing = Γ ^ 1 201117820, it is only equivalent to about 40% of the activation effect of the positive control group. It is worth mentioning that the mixed camphor extract Ml in Fig. 2 has better effects than the dried or concentrated alcohol extract (Fig. 3) and the dried or concentrated water extract (Fig. 4). The above is intended to be illustrative only and not limiting. Any equivalent modifications or alterations to the spirit and scope of the present invention are intended to be included in the scope of the patent application. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart showing the preparation steps of a toon extract according to a preferred embodiment of the present invention; and FIG. 2 to FIG. 5 are various doses of the various extracts of the present invention and the dried camphor tea. A graph of the luminescence intensity data presented after the treatment. [Main component symbol description] • A1: alcohol extraction mixture All: alcohol extract A12: alcohol extract H1: water extraction mixture H11: water extract:
Ml:混合的香椿萃取物 ： S10〜S61 :步驟 18Ml: Mixed Toon Extract: S10~S61: Step 18