TWI379684B - - Google Patents

Description

1379684 24570pi f1 is the Chinese manual of No. 96119746. There is no slash correction. The present invention relates to a new Maitake extract and a composition for promoting sebum production comprising the same. . [Prior Art] The gland is attached to the skin and can be divided into an oily substance called a leather wall to form a non-permeable protective flaw on the surface of the epidermis. Sebum is formed from a mixture of lipids, which contains glycerol

0 Guang, Saki, aliphatic wax, free fatty acids, cholesterol and so on. In addition, sebum also has a moisturizing effect, which makes the epidermis soft, and at the same time, sebum plays an important role in enhancing skin strength. Lipid also protects against epidermal damage caused by UV rays, protects the skin 3 ^, and invades the allergens. Not only foreign matter and due to age and (4) _, etc., the sebum produces this force, which causes the skin's heterogeneity and strength to decrease. In particular, sebum produces resistance to scallops

Corrected the peak of the skin on August 24, 2010. The decline 'is also the skin makeup / field eye makeup, oleic acid and broadcast report) 4 as a promotion of sebum production called ingredients has been used by people like hot water extraction of Maitake extract (Special π bulletin, (4) No. 7 Bulletin and Special Opening Ceremony - No. 7 Bulletin) or 2457 pifi of water-containing alcohol extraction, revised from January 24, 2011 to No. 96191974 Chinese manual without scribe line correction (Special Kaiping 8-131133) Bulletin and special opening 2, _319192, etc., and extracts of fresh materials taken from water or aqueous baths have been known. The water-soluble dissolved extract can be (10) specially developed for the prevention of cancer. Japanese Patent Publication No. 1978978, anti-oxidant (Specially Open 2: 5 22_7 1 reported), antifungal agent (Japanese Unexamined Patent Publication No. Hei 2 No. Hei. No. Hei. No. Hei. SUMMARY OF THE INVENTION The object of the present invention is to provide a Maitake extract which is novel and more effective in promoting the production of sebum and a composition containing the same. y The present inventors have solved the above problems. After conducting a serious investigation and research, it was found that the dry (four) extract obtained by pure extraction has a strong effect of promoting the skin growth, and the present invention has been completed. That is, the present invention is as follows. (1) A maitake The extract is extracted from dry maitake with pure alcohol. (2) The water content of dry maitake used in the maitake extract described in (1) is 8 wt% or less (wt%) (3) The alcohol content of the pure alcohol used in the maitake extracts described in (1) or (2) is 99% or more by volume. (4) Contains any of (l)-(3)t The composition containing the Maitake mushroom extract to promote the production of sebum described in item i. (5) Record in (4) (6) The composition described in (4) is a pharmaceutical product. The present invention can effectively promote the production of sebum, and effectively improve 2457 〇pifl to the Chinese manual of No. 96119746. The above-mentioned and other objects, features and advantages of the present invention will become more apparent from the above-mentioned and other objects, features and advantages of the present invention as a result of the reduction of sebum on August 24, 2011. The embodiment will be described in detail below with reference to the accompanying drawings. [Embodiment] The present invention relates to a Maitake mushroom extract obtained by extracting from dry maitake with pure alcohol. , refers to the fungi of the genus Polyporaceae and Grifola, which include Griffola frondosa, Grifola albicans, porcine ((^咐um umbeuatus), sub-ash tree flower ( Grif〇ia gigantean), etc. The most ideal of the maitake of the present invention is Grif〇la frondosa. The maitake of the present invention may be a fruiting body or a mycelium, but ideally refers to a fruiting body. The present invention is dry. Dancing The raw material to be extracted, and the dry maitake is a maitake with a t content of 10% by weight or less (Wt%). Ideally, the moisture content is 8 wt% or less = the dance content is 7 wt% or less. The dry velvet is;; 任思的方邮Π阴干,加热赖, the fresh 舞茸 is taken off. ～~干干垛4) Refers to the powder (four) drying it. The most ideal dry maitake in the invention Pure alcohol means that in the day: =::=: think _ fine ==,; the alcohol, the most ideal pure alcohol refers to the wine 24570pifi revised the flood season August 24, the 96th 丨 6 6 6 6 6 丨_ _1 £ £ The essence content is alcohol with a capacity of 99.5% or more. The pure hydrazine extraction method of the dry material is carried out in the supplement (dry velvet), and is carried out at normal temperature or in the heating condition T. The stirring temperature for the fixed time is usually the temperature below the _ axis, but in the sealing capacity; The temperature below the corpse is also acceptable. The ideal extraction temperature is room temperature (room temperature). The extraction time is not particularly limited, and is, for example, about 5 minutes to 1 hour. The number of extractions can be performed 1 to 2 times. In the case of extracting, when the dry maitake is 100 parts by weight, the amount of pure and fine is 丨〇〇~丨〇〇〇 by weight, preferably 2〇〇~6〇〇, The most desirable is 300 to 5 parts by weight. After the completion of the sheet extraction treatment, it is filtered by a filter paper or a filter cloth or a separation means such as centrifugation to obtain a pure alcohol extract. The Maitake pure alcohol extract in the present invention may be in the above-described alcohol extract state. Alternatively, the pure alcohol extract may be an extract obtained by completely or partially removing alcohol from the extract using a usual method. That is to say, the pure muslim extract of the present invention may be an extract powder containing no alcohol or an extract having an alcohol content of 1 to 5 % by weight. Among them, an extract having an alcohol content of 10 to 15% by weight is preferable because of its good preservability. The pure alcohol extract is obtained in an amount of 2 to 10% by weight of the dry maitake, and it can be more certainly obtained by the amount of 3 to 5% (solid content) of the dry maitake weight. Pure alcohol extracts contain phospholipids and phytosteroids, but the active ingredients they contain are not yet certain. The present invention contains a composition which promotes sebum production by the above extract. 1379684 ' 24570pifl - For the 96th test No. 6 Chinese manual without a slash correction This correction date is August 24, 2011. The composition of the invention promotes sebum production and keeps the skin epidermis moist, giving the skin a moisturizing and soft skin. At the same time, it can also enhance the strength of the skin. Moreover, it also protects the epidermis from ultraviolet rays. Protects the skin from foreign bodies and allergic sources. Accordingly, the present invention contains a composition which promotes sebum production by the above extract, and may be, for example, a cosmetic. The composition of the present invention is a cosmetic containing a carrier, an excipient, an additive, and the like which are conventionally used in cosmetics, and can be used as a skin care agent for preventing dryness of the skin, keeping the skin delicate and the skin soft, improving the dryness of the skin, and the like. Product use. It can also be applied to cleansing the face (such as face cream, foam, cream, etc.), skin type '% (makeup, beauty liquid, etc.), skin care (lotion, moisturizing cypress), mask, oil, massage, Make-up base (powder cream, sunscreen lotion, etc.), topical make-up (lipstick, eye shadow, eyeliner, etc.), bath (soap, body wash, bath, etc.), sunscreen lotion, etc. · Cosmetics such as hair growth (hair growth, hair growth, etc.). Among these cosmetics, the amount of maitake extract that can be effectively used is as follows: • In the range of 〇_1~99.9 wt〇/〇, it is ideally 疋1 to 99 wt%. between. In the remainder, the carriers, excipients or additives are used in combination with each other. The extract ' of the present invention has symptoms of improving skin P early damage or skin diseases caused by a decrease in sebum production. Therefore, the extract of the present invention may also be a pharmaceutical for external use, which comprises a composition composed of the above extract and having a function of promoting sebum production. The extract of the present invention may, for example, be a pharmaceutical for external use, which contains a carrier, an excipient and an additive which are conventionally used in the Chinese Patent Specification No. 1379684, 24570 pifl, August 24, 1989. Due to the reduction of sebum, bowel skin disorder or skin disorders such as dry skin, sebaceous dermatitis, anaesthetic dermatitis, etc. The form of the drug includes: =, 'storage suspension, lotion, cream, ointment, gel, and the like. In these external preparations: 乂, if the extract is used to convert the effective amount of Maitake extract, between 〇 1~Q, ^' wt%, it is desirable to mix between 1 and 99 wt%. In the remaining *8, the commonly used carriers, excipients or additives are mutually formulated. In cosmetics or pharmaceuticals, commonly used carriers, shaping or additives, etc., can be, for example, solvents, vegetable oils (for example, almond 4 = 蓖 vegetable oil, cocoa butter, coconut oil, corn oil, brocade Seed oil, flaxseed, edible oil from eucalyptus oil, palm oil, peanut oil, poppy seed oil, vegetable oil, sesame oil, soybean oil, sunflower oil and tea oil, mineral oil, fatty oil, liquid = oil, buffer Agents, preservatives, humectants, chelating agents, antioxidants, stabilizers, emulsifiers, suspending agents, gels, ointment bases, sachet bases, penetrants, fragrances, and skin care agents. The above solvent is, for example, water, alcohol, polyethylene glycol, propylene glycol, glycerin, liquid polydidecyloxyne, and a mixture thereof, but is also limited thereto. The buffer of the present invention is, for example, citric acid, acetic acid, tartaric acid, lactic acid, hydrazine hydrogen, ethylenediamine, or a mixture thereof, but is not limited thereto. ^ The above-mentioned carrier, such as glycerin, propylene glycol, pentanediol, sorbitol * chylo urea, 13 - butane diol (13 butylene gly C 〇 13 BG), soy solid = and their present compounds, but It is not limited to this. The above-mentioned preservatives' are, for example, sodium EDTA, citric acid, and their 1379684 '24570pifl - are the Chinese manual of No. 96119746, and the mixture is not slashed, and the correction date is August 24, 2011, etc., but is not limited thereto. The above antioxidants are, for example, butylated hydroxyanisole 'BHA, vitamin C and its quinone conductor, vitamin E and its inducer, cysteine and mixtures thereof, but It is not limited to this. The above emulsifiers are, for example, natural gums (for example, locust bean gum), tragacanth, sheet gum, natural phospholipids (for example, soybean squamosa); polysorbate 80 inducer; lanolin; lanolin alcohol; A sorbitan ester; a monoglyceride; a fatty alcohol (eg, 'behenyl alcohol); a fatty acid ester (eg, tris(octanoic acid/kuric acid) glycerol vinegar), similar to a monostearic acid (SE)-like fatty acid Triglycerides and mixtures thereof, but are not limited thereto. The above suspending agent is, for example, cellulose and an inducer thereof (for example, carboxymethyl cellulose 'hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxypropyl fluorenyl cellulose, etc.), horn gum, locust bean Gum, gum arabic, tragacanth gum, and mixtures thereof, but are not limited thereto. The above gel base and tackifying component are, for example, liquid paraffin oil, polyethylene, fatty oil, colloidal enamel, colloidal aluminum, zinc soap, glycerin, propylene glycol, tragacanth, and styrene vinyl polymer (carb 〇). Xyvinyl p〇iymer), magnesium aluminum alumite, hydrophilic polymer (for example, cellulose, carboxyethyl cellulose, hydroxyethyl cellulose, and cellulose inducer such as cellulose inducer), water swellable hydrophilicity Colloids, carrageenan, hyaluronic acid salts (for example, hyaluronic acid gels optionally containing sodium chloride), alginic acid esters (eg, alginic acid propylene glycol vinegar), and mixtures thereof, etc., but not Limited to this. The above ointment bases, for example, beeswax, paraffin, alcohol, cetacein, 1379684 24570pi f1 for the 96119746 revision date August 24, 2011, 99.5%) 'after about 18 hours, at 2〇t: The extraction was carried out while stirring at a temperature. Then, the residual/checking method is removed by centrifugation, and the obtained supernatant clear solution is filtered with a filter paper, and then the alcohol in the filtrate is removed by means of bursting and removing, thereby obtaining the pure gynosolic alcohol extract. (production: 43.2 g, solid content: 37.2 g, alcohol: 6.0 g). [Experimental example] The effect of pure alcohol extract of Maitake mushroom on promoting sebum production (Method)

1. In vitro method U guinea pig sebaceous gland cell culture method 1-M guinea pig sebaceous gland cell isolation Male golden guinea pig (5 weeks old, purchased from Japan SLC company) the ear and right ear of the ear shell was removed 'and soaked In a DMEM (Dulbecco's modified Eagle's medium) culture solution (manufactured by Invitrogen Co., Ltd.) to which penicillin and streptomycin sulfate were added, and left at 4 ° C for about 1 hour. Then, it was washed with a phosphate buffer (PBS(-)). Next, the hair around the ear shell was removed, and the ear shell was cut into pieces of 5 x 5 mm 2 and allowed to stand at 4 ° C for 13.5 hours in a 2.4 unit/ml Dispase solution (manufactured by Contract Alcohol Co., Ltd.). After treatment with Dispase solution, the epidermis of the ear shell was peeled off by the recording, and the dermis with sebaceous glands was immersed in Sebocyte growth (SG) medium [6% (v/v) calf serum (manufactured by Nichirei Bioscience) / 2% (v/v) human serum (produced by ICN Biomedical LTD / 68 68 mM L-Glutamine / 100 Units / ml penicillin and l 〇〇 pg / ml streptomycin / DMEM / F-12) under the microscope Separation of sebaceous gland cells. 11 1379684 24570pi f1 Revision date August 24, 2011 is the Chinese manual No. 96191974. No slash correction. 1-1-2 Tissues of guinea pig sebaceous gland cells. Mouse 3T3 cells (purchased from Physicochemical Research and Development) Bank) After treatment with mitomycin for 4 hours, the cells were seeded at -10 mm cells in a cell size of 1×103 cells/cm 2 and cultured into a monolayer as a supporting cell layer for culturing guinea pig sebocytes. The isolated sebaceous glands were placed on the support layer cells and cultured at 37 ° C '5% C 〇 2 until the cells were increased to near intensity. The cell culture medium was supplemented with EGF (10 ng/ml). SGmedium 'Change culture around 2 days Subculture of 1-3 guinea pig sebaceous gland cells The mouse 3T3 cells supporting the cell layer were removed with 〇.〇2〇/0(w/v;) EDTA/PBS(·) solution, and then proliferated. The guinea pig sebaceous gland cells grown into tissue pieces were exfoliated with 〇.25% (w/v) trypsin/〇.〇2% (w/v) EDTA/PBS(-) solution, and subcultured. None of the subcultured cells used in the experiment were more than 3 generations. 1-2 Treatment method The day after the addition of the mouse sebocytes to the 35 mm culture dish, with or without the addition of temsin (10 nM) (Sigma) -In the case of Aldrich, the maitake extract (100, 200 and 400 pg/ml) obtained in Example 1 and the extract containing Brazilian mushrooms (1〇〇, 2〇〇 and 400 pg) were used. /ml) of culture medium [6% calf serum/2% human serum / 0.68 mML-valine / l〇〇units / ml penicillin G / 100pg / ml streptomycin / DMEM / F-12] on the cells The treatment was carried out. After 3 days, the same treatment was carried out, and then the culture was continued for another 3 days. The cells used in the whole experiment were subcultured for no more than 3 passages.

S 12 1379684 2457〇pifl Revised date August 24, 2011 is No. 96119746 Chinese manual No scribe correction 1-3 Oil red 染色 Dyeing method contains 0.3% oil red 0 (Sigma-Aldrich) isopropanol The (isopiOpanol) solution and the double distilled water were mixed at a ratio of 3:2 (v:v), and placed in a sealed ultrasonic cell pulverizing apparatus for ultrasonic treatment for 15 minutes. It was then placed at room temperature for 1 minute and filtered. The oil red 〇 of the filtered supernatant solution was used as a staining solution. After the cells were washed with pBs(_), a fixing solution [4〇/0 polyformic acid (Paraformaldehyde)/PBS(-)] was added and fixed at room temperature for 1 hour. After fixing, the cells were washed with hot water, and the oil red sputum staining solution was added and stained at 37 C for 15 minutes. After the staining, the cells were washed with running water to clean the formation of fat droplets by an optical microscope. Also, the cells stained with oil redO were photographed as digital images under optical microscopy.

For example, the quantitative software Lumina Vision Ver2.2.2 (manufactured by MITANI CORPORATION) quantifies the proportion of fat droplets accumulated in cells in three different places in each treatment, and the quantification is expressed in units of artifacts. Quantification of endothelial lipid content of φ 1-4 cells 1) Sample preparation The drug-treated cells were washed twice with PBS (·), and a solution of 0.25% trypsin/0.02% EDTA/PBS(-) The recovered, frozen recovered cell suspension is subjected to ultrasonic treatment to pulverize the cells. 2) Determination of the amount of triglyceride using TGII (Roche Diagnostics) to measure the amount of triglyceride (TGs), the main component of sebum, that is, the cell pulverization sample in the above "1)" 13 1379684 24570pifl is No. 96119746 Chinese manual no slash correction This correction date is added to the product on August 24, 101. [1.65 IU/ml glycerolkinase/6 IU/ml glycerol-3 -phosphate oxydase/catalase/2.95 μιηοΐ/ml disodium adenosin-5' -triphosphate, trihydrate (ATP) yO.65 μιηοΐ/mlsodium N-(3 , 5-dimeriioxyphenyl)-N'-succinylethylenediamine (DOSE)], allowed to react at 37 ° C for 10 minutes to remove free glycerol. Immediately thereafter, add the treatment solution 2 [0.55 IU/ml lipoproteinlipase/peroxidase/0.65 μιηοΐ/ml 4-aminoantipyrin (4-AA)], and let it be at 37. . The reaction was carried out for 10 minutes. After the reaction was terminated, the absorbance was measured at 590 nm. The amount of TGs was calculated by the absorbance of the triolein standard solution (0.6 mg/ml). Further, the content of DNA in the pulverized sample was measured by the 3,5-diaminobenzoic acid dihydrochloride (DΑΒΑ) method, and the content of TGs in 1 gg DNA was calculated. 2. In vivo experimental method The male guinea pig (purchased from SLC, Japan) (the treatment group, n=l in each group) of the ear shell (left ear) was coated with Maitake extract and contains Brazilian mushroom extract. Solution (95% ethanol / 5% glycerol), once a day, 50 μg each time, the same guinea pig's right ear was coated with a solution containing no Maitake extract and containing Brazilian mushroom extract (95 % ethanol / 5% glycerol) (control group, each group η = 1). After 2 weeks, the ear shell portion of the dolphins was made into a section of 'Kangjie' and subjected to oil recj 〇 staining. Under the optical microscope, the tissue slice of the ear shell portion stained with oil redO is photographed, and the image is analyzed and quantified. Lum II = 1379684 24570pifl Corrected date August 24, 2011 is No. 96119746 The line corrects the proportion of the drop quantitative 'the quantification is expressed in units of portraits. 3. Statistical processing The Fisher multivariate dispersion analysis method was used to perform the verification of significant differences between treatments. (Result) 1. In vitro experiment

(1) Cellular groups (low-differentiated) that were not treated with insulin, Maitake extract promoted the formation of intracellular fat droplets (Fig. 1B, 400 ng/ml; compared with Fig. 1A). At the same time, it was confirmed that the promotion of fat droplet formation by Maitake extract is a concentration-dependent relationship (Fig. 2). On the other hand, Brazilian mushrooms faintly promoted the formation of intracellular fat droplets (Fig. lc, 4〇〇^g/ml; compared with Fig. 1A and Fig. 2), and its promotion effect was significantly higher than that of Maitake extract. The ground is weak (ρ < 0.001). (2) Insulin-treated cells (differentiated), Maitake extract also promoted the formation of intracellular fat droplets in a concentration-dependent manner (Fig. 1; compared with Fig. ID and Fig. 2). On the other hand, the Brazilian mill extract under the same conditions promoted the formation of intracellular fat droplets (Fig. 1F; compared with Fig. 1D and Fig. 2) but it was significantly more effective than Maitake extract. The ground is weak (p < 0.001). (3) The concentration of Maitake extract increased the amount of intracellular triglyceride in the cell population without insulin treatment (Fig. 3). However, under the same conditions, the Brazilian mushroom extract did not alter the intracellular triglyceride content (Figure 3). On the other hand, the insulin-treated cells, the concentration of Maitake extract, increased the intracellular triglyceride content in the same concentration (Fig. 15 1379684 24570pifl is No. 96119746 Chinese manual no underline correction this revision period 101 years 8 Month 24曰3). However, the Brazilian mill extract also increased the intracellular triglyceride content, but at the concentration of 400 pg/mi, the increased triglyceride content was significantly lower than that of Maitake extract. (image 3). 2· In vivo Experiment (1) The following table shows the changes in body weight before and after treatment with Maitake Extract and Brazilian Mushroom Extract. Maitake Extract Brazil Mushroom Extract Concentration - 1% 2% 4% 1% 2% 4% Flat Guinea Pig 1 Guinea Pig 2 Guinea Pig 3 Guinea Pig 4 Guinea Pig 5 Guinea Pig 6 Pre-buried (a) 42.3 g 47.9 g 45.2 g 47.3 g 46.8 g 46.3 g after treatment (b) 84.1 g 92.0 g 81.0 g 92.0 g 85.0 g 86.0 g ^*jt (ba) 41.8 g 44.1 g 35.8 g 44.7 g 38.2 g 39.7 g (2) Maitake extract at 1%, At the concentrations of 2% and 4%, the sebum accumulation in the sebaceous gland tissue was promoted by 1.6 times, 3 times, and 1.3 times, respectively (Fig. 4 and Fig. 6A). (3) 1% of the Brazilian mushroom extract inhibited sebum accumulation in sebaceous gland tissue while other concentrations (2% and 4%) showed no significant change in cortical accumulation (Fig. 5 and Fig. 6A). (Discussion) In in vivo and in vitro experiments, it was apparent that the Maitake extract has an effect of promoting sebum production. At the same time, the fact that the promotion of Maitake extract is significantly stronger than that of Brazilian mushrooms has been confirmed. In other words, the maitake extract is a substance that strongly promotes the production of sebum, and has a certain effect not only on the maintenance of dry skin, but also on the symptoms of skin diseases caused by a decrease in sebaceous gland function such as skin dryness. 16 1379684 - 24570pi f1 Revision date August 24, 2011 is the Chinese manual No. 96191974 No-line correction [Example 2] The promotion of triglyceride (TGs)-specific production of pure alcohol extract of Maitake ( Method) 1. Culture of guinea pig sebaceous gland cells The guinea pig sebaceous gland cells were seeded in a 35 mm culture dish at a cell number of 2.35 x 104 cells/cm 2 , and the maitake extract obtained in Example 1 (100, 200, and 400 pg/ Ml) alone or in combination with insulin (1〇nM)® adipose cell culture medium (SBCM) [DMEM/F-12/6°/〇(v/v) calf serum/2% (v/v) /0.68 mM L-valine / 100 units / ml penicillin G / 100 pg / ml streptomycin sulfate culture medium 3 曰. After 3 weeks, the same preparation of the same Maitake extract or the addition of mesin (10 nM) to the lipocyte culture medium (SBCM) for another 3 days. The control group was a lipocyte cultured in a culture medium (SBCM) containing no Maitake extract and insulin. 2. Analysis of sebum composition • The adipose gland cells cultured with broth (SBCM) containing or without Maitake extract were washed twice with PBS (_) for 25.25% (w/v) trypsin/0.02 %(w/v) EDTA/PBS (-) solution was used to recover cells. The recovered cell suspension was placed in ice water and subjected to ultrasonic treatment for 5 minutes (200 W, 6 seconds) using a sealed ultrasonic cell (Bioruptor's COSMO. Bio Inc.) to prepare a sebum composition analysis material. The material was transferred to a degreased Spitz tube and a solution of methanol (2: hydrazine; v: v) was added to extract the lipid. The extracted lipid was analyzed by an automatic thin layer chromatography instrument (manufactured by TLc MATRON Co.). The analysis method is that the extraction method 17 1379684 24570pifl is the Chinese manual of No. 96191974. There is no slash correction. The correction date is August 24, 2011. After the mass point is in the thin layer of the rod, a solution of oxime: sterol (35: 35) is added. 1 development (1.5 cm); 2 developments (8 cm) with benzene:formaldehyde:acetic acid (50:20:0.7); further expansion with hexane:benzene (35:35) (U cm ). Then, the lipid separated on the rod-shaped thin layer was directly detected by a Flame Ionization Detector's HD at room temperature at a rate of 30 sec/rod. In addition, the composition of each lipid was analyzed by a liquid chromatograph using a known amount of a standard lipid control, such as 3-palmitoglyceride (corresponding to TG of Figure 7); palmitate (corresponding to FFA of Figure 7) Cholesterol (corresponding to Cho of Fig. 7); palm citrate cholesterol (corresponding to ChoE of Fig. 7) (produced by Do〇san Serdary Research Laboratories); and palmitic acid (corresponding to Wax E of Fig. 7) (Nu- Produced by Chek PreP Inc.) Further, each lipid was quantitatively calculated using cholesteric acetate (2 pg) (manufactured by Doosan Serdary Research Laboratories) as the peak area of the internal standard. (Results) Based on the results of the cortical composition analysis, it was confirmed that the Maitake extract (100, 200, and 400 pg/ml) firstly increased the content of the triglyceride in the cortical component in a concentration-dependent manner (Fig. 7). In addition, Maitake extract increased the content of cholesterol ester (ChoE) and free fatty acid (FFA) at a concentration of 400 pg/ml. Their content is 2.2% and 5.3% in the total fat content, respectively, and is significantly lower than the 81.5% triglyceride. [Example 2] The following is a product containing a Maitake mushroom skin cream (as a beauty product) 1379684

24570pifl is the Chinese manual of No. 96119746 without a slash correction. The date of amendment is August 24, 2011. Formulation amount (wt%) Maitake extract 2.0 Olive oil 10.0 Triglyceride (octanoic acid / sunflower acid) ester 10.0 Vitamin E 1.0 Refined water 77.0 Total 100.0 [Example 3] The following '疋 contains Maitake extract skin cream ( Formulated as a pharmaceutical). Ingredient name (wt%) Maitake extract 10.0 撖 油 10.0 glycerol tris(octanoic acid / sunflower acid) 酉 10.0 Vitamin E 10 purified water 69.0 total 100.0 [Example 4] Maitake extract for patients with dry skin The effect of the action was as shown in Example 2, and a dry skin disease patient was subjected to experimental treatment with a skin cream containing 2% by weight of Maitake extract to observe the effect. Test method Test subjects: This test is for the elderly living facilities (G〇〇dWell) 19 1379684 24570pifl for the 96th 丨 丨 6 6 6 丨 丨 雠 雠 雠 雠 雠 雠 雠 患有 患有 患有 患有 患有 患有 患有 患有 患有 患有 患有 患有 患有 患有 患有 患有 患有 患有Skin disease, 12 patients with desquamation (76-97 years old, including 6 males and 6 females). During the implementation period: December 2, 2007 to January 2007 匕 = method: divided into paragraphs based on desquamation (symptoms of exfoliation of the skin) (3 · · severe cases; 2: moderate symptoms; 1: light After the evaluation of the skin of the test subject, the application of the velvet extract on the skin of the testee

Skin cream. The application site includes: a right lower wrist, a lower left wrist, a lower right leg, and a lower left leg, and is evaluated on this portion. Results The skin was not tested by the tester 1 week after the start of the smear test == Jing, degree (3: severe; 2: middle disease; ι: outside, β break # degree. 0' means

Subject 1 Examination and application 曰2006/12/4 Right lower wrist Left lower wrist Right lower leg Left lower leg 2006/12/6 2 1 2 2 2006/12/8 1 1 1 1 2006/12/11 1 1 1 1 1 0 1 1 Subject 2 Examination and application 曰2007/1/17 Right lower 婉1 Left lower wrist Right lower leg Left lower leg 2007/1/19 1 2 1 2007/1/22 1 1 1 1 2006/12/11 0 0 1 1 0 0 1 1

S 20 1379684

245/Upitl is the Chinese manual of No. 96119746. There is no slash correction. The date of revision is August 2, 2010. The testee 3 The examination, the application, the right lower wrist, the lower left wrist, the lower right leg, the lower left leg 2006/12/6 2 2 2 2 2006/12 /11 2 2 1 2 2006/12/13 1 0 0 0 Subject 4 Examination · Smear 曰 Right lower wrist Left lower wrist Right lower leg Left lower leg 2006/12/15 1 1 1 1 2006/12/18 0 1 0 0 2006/12/20 0 1 0 0 2006/12/22 0 0 0 0 Subject 5 Examination · Smear 曰 Right lower wrist Left lower wrist Right lower leg Left lower leg 2007/1/5 2 2 3 2 2007/1/8 1 1 2 2 2007/1/10 0 0 2 2 2007/1/12 0 0 2 2 The testee 6 examined and smeared 曰 right lower wrist left lower wrist right lower leg left lower leg 2006/12/18 0 1 3 2 2006/12 /11 0 0 2 1 2006/12/13 0 0 1 1 Subject 7 Examination · Smear 曰 Right lower wrist Left lower wrist Right lower leg Left lower leg 2006/12/18 1 1 1 1 2006/12/20 0 0 1 1 2006/12/20 0 0 1 1 2006/12/22 0 0 0 1 21 1379684 24!)/Upiil is the Chinese manual of No. 96119746. There is no slash correction. The date of this amendment is August 201. The testee 8 is inspected and smeared.曰 right lower wrist left lower wrist right lower leg left lower leg 2006/12/29 2 3 3 3 2006/12/30 1 2 2 2 2007/1/4 1 0 2 1 Testee 9 Inspected and smeared 曰 Right lower wrist left lower wrist Right lower leg left lower leg 2007/1/5 0 0 2 3 2007/1/8 0 0 2 1 2007/1/10 0 0 2 1 2007/1/12 0 0 1 1 Subject 10 Examination · Smear 曰 Right lower wrist Left lower wrist Right lower leg Left lower leg 2006/12/20 1 1 2 2 2006/ 12/22 1 0 2 2 2006/12/25 1 0 1 1 2006/12/26 1 0 1 1 2006/12/27 1 0 1 1 Subject 11 Examination and application 曰 Right lower wrist left lower wrist Right lower leg lower left Leg 2006/12/4 1 1 2 1 2006/12/6 0 1 1 1 2006/12/8 0 1 1 1 2006/12/26 0 1 0 0 Testee 12 Inspected and smeared 曰 right lower wrist left lower wrist Right lower leg left lower leg 2006/12/18 0 1 2 1 2006/12/20 0 0 1 1 2006/12/22 0 0 1 1 2006/12/25 0 1 0 0

As shown in the above diagnostic evaluation degree, the skin condition of the subjects 1 to 12

S 22 1379684 24570pifl Revised date August 24, 2011 For the Chinese manual No. 96191974, there is no slash correction. The state is improved after 1 week (7 曰) after application. In addition, the improvement effect of the skin cream applied with the velvet extract can be observed after 1 to 3 days of application. Fig. 8 is a photograph of the subject 5 before and after the treatment with the mascara extract cream. The dry skin improvement effect of the skin cream after applying the maitake extract can be confirmed from these photographs. Based on the above results, it was confirmed that a skin cream containing 2 wt% of Maitake extract can have a therapeutic effect on dry skin disease. The extract of the present invention has an action of promoting sebum production, and can be used as a cosmetic or a medicine to effectively treat or ameliorate skin abnormalities, disorders, or skin diseases caused by a decrease in sebum. While the present invention has been described in its preferred embodiments, the present invention is not intended to limit the invention, and the present invention may be modified and modified without departing from the spirit and scope of the invention. The scope of protection is subject to the definition of the scope of the patent application. [Simple description of the diagram] Figure 1 shows the effect of Maitake extract and Brazilian mushroom extract on the formation of fat droplets in guinea pig adipose cells, which shows the formation of fat droplets of adipose gland cells by Maitake extract and Brazilian mushroom extract. The effect of the photo. Fig. 2 is a graph showing the dynamics of the amount of sebum in the murine adipose gland cells treated with Maitake extract and Brazilian mushroom extract, which shows a graph of the amount of sebum in the lipocytes after treatment with Maitake extract and Brazilian mushroom extract. Among them, '** and ***, and the untreated cells (C) were significantly different (p < 0.01 and 0.001); ##和###, and after the insulin 23 1379684 24570pifl correction period 101 years 8 Month 24曰 is the No. 119746 Chinese manual without a scribe line: 1:; Significant differences in physiology (p < 〇〇 1, and 〇〇〇 1). Fig. 3 is a graph showing the effect of Maitake extract and Brazilian mushroom extract on the amount of TG in guinea pig adipose cells, which shows a graph showing the effect of Maitake extract and Brazilian mushroom extract on TG production in sebaceous gland cells. Among them, ** and ***, and untreated cells (C) were significantly different (p < 0.05 and 〇. 〇〇 1); #, ##和###' and significantly treated by insulin-treated cells Sexual differences (p<〇.〇5, 0.01 and o.ooi). Fig. 4 is a graph showing the effect of Maitake extract on sebum accumulation in guinea pig sebaceous glands using Oil red 〇 staining, which shows a photograph of the effect of Maitake extract on sebum accumulation in the serotonin gland. Among them, the black ellipse in the tissue section refers to the sebaceous gland, which is represented by (▲); the side of the velvet extract is coated; ▲: sebaceous gland. Fig. 5 is a graph showing the evaluation of the effect of the Brazilian mushroom extract on the sebum accumulation of the guinea pig sebaceous gland using the Oil red Ο staining method, which shows a photograph showing the effect of the Brazilian mushroom extract on the sebum accumulation of the sebaceous gland. Among them, the black ellipse in the tissue section refers to the sebaceous gland, which is represented by (▲); *: the side of the extract of the Brazilian mushroom extract; ▲: the sebaceous gland. Lutu 6A and FIG. 6B show the dynamics of sebum content in guinea pig sebaceous gland tissue after treatment with Maitake extract and Brazilian mill extract, which shows a graph of sebum content in lip gland cells after treatment with Maitake extract and Brazilian mushroom extract. . Here, Fig. 6A is a treatment of Maitake extract, and Fig. 6B is a treatment of a mushroom extract of Brazil, and ** and *** are ρ < 〇.〇1 and 0.001. Figure 7 is a graph showing the analysis of the composition of sebum in the guinea pig sebaceous gland tissue after treatment with Maitake extract, which shows the guinea pig sebaceous gland group treated with Maitake extract.

S 24 1379684 24570pi f1 is the Chinese manual of No. 96丨19746. There is no slash correction. This correction date is the chart of the analysis of the sebum composition in the woven fabric. ChoE, cholesterol ester (corresponding to the standard lipid used as palmitic acid cholesterol); WaxE 'wax ester; (corresponding to standard lipid and palmitic acid cholesterol used); TG, triglyceride (as standard lipid and used brown Acid cholesterol corresponds to) FFA, free fatty acid (corresponding to standard palmitate and palmitate cholesterol used) Ch〇, cholesterol (corresponding to standard lipids as used cholesterol).

The quantitative dispersive analysis method was used for statistical analysis, and the difference between the treatment groups was used to identify the differences. * and *** are shown in the cultured (10) rat sebaceous gland cells treated with sputum (the sera of the county are compared after the sputum is taken. The composition is shown in Figure 8. The symptoms are improved (No. 5 is tested, respectively) :: Extraction No. 5 test before and after treatment of skin cream; [Key component symbol description]

25

Claims (1)

1379684 Amendment date August 24, 24, 24, pipifl, No. 961, 1974, Chinese manual, no underline correction. 7. Scope of application: 1. A sebum production promoting substance, which uses alcohol with a capacity of 98% or more in terms of capacity. ～1000 parts by weight, obtained by extracting 1 part by weight of a fruit body of dry maiko (Grif〇la fr〇nd〇sa) having a water content of 6 wt% or less. 2. A cosmetic product comprising a sebum production promoting substance as described in claim 1 of the patent application. 3. A pharmaceutical product 'containing a sebum production promoting substance as described in claim 1 of the patent application. A method for producing a sebum production-promoting substance, comprising: using 400 to 1000 parts by weight of alcohol having an alcohol content of 98% or more in terms of capacity, and a fruiting body of dry maitake (Grifolafrondosa) having a water content of 6 wt% or less 1 part by weight for extraction. A method for producing a cosmetic for promoting sebum production, comprising: using 400 to 1000 parts by weight of alcohol having an alcohol content of 98% or more calculated by volume, and a fruiting body 100 of dried Maitake with a water content of 6 wt% or less The parts by weight are extracted. 6. A method for producing a pharmaceutical product for promoting sebum production, comprising: using 400 to 1000 parts by weight of alcohol having an alcohol content of 98% or more calculated by volume, and a dry Maitake mushroom having a water content of 6 wt% or less 100 parts by weight of the solid was extracted.