TWI318117B - Preventive agent against damage from forced molting - Google Patents

Description

1318117. (1) 发明 发明 发明 发明 发明 发明 发明 发明 发明 发明 发明 发明 发明 发明 发明 发明 发明 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制 强制method. [Prior Art] The spawning rate of laying chickens will decrease with age, and the egg weight will be too large and easily broken, mostly eggs with low commercial prices. For such a chicken, the feed is given for a certain period of time or the feed or water supply is stopped to stop the spawning, and the chicken itself is regenerated after the feather is pulled out again. This treatment is called forced hair change and is a treatment method widely practiced by the egg industry to improve the economics of egg production. Most of the flocks that are forced to change feathers increase the rate of egg production and egg quality, but on the other hand, as the feed supply is stopped, there is damage, or death or inability to lay eggs. Further, the defensive function against the harmful bacteria causing the related obstacles is lowered, and the possibility of causing egg contamination is increased. Therefore, for poultry such as chickens, an effective forced feathering method that does not cause such an obstacle is expected. However, for chickens, lactic acid bacteria such as Lactobacillus reuteri are known for the purpose of inhibiting the proliferation of Salmonella (Japanese Unexamined Patent Publication No. Hei No. Hei. No. Hei. Bulletin 0274). However, for adult chickens, the method of administering lactic acid bacteria for the purpose of suppressing the obstruction of forced feathering is still unknown. -5 - 1318117 (2) SUMMARY OF THE INVENTION An object of the present invention is to provide a method for effectively forcibly changing feathers and forcibly changing feathers for poultry under the barrier of being disabled.

The inventors of the present invention have made various review results for the above-mentioned purposes, and found that there is a serious cell damage in the intestine of a poultry that has been subjected to forced feathering, and the damage caused by hunger when the feather is forcibly changed can be administered by a defensive agent containing lactic acid bacteria. And significantly improved. The present invention provides an agent for preventing forced feather damage by using lactic acid bacteria as an active ingredient. Further, the present invention provides a forced feather changing method comprising the step of fasting the poultry and the step of administering the above-mentioned agent for forcibly changing feather damage to the poultry.

[Embodiment] The preferred embodiment of the present invention is a medicinal agent for preventing feather damage, which comprises lactic acid bacteria as an active ingredient, and the lactic acid bacteria preferably have a form in which a living bacterium is administered to a subject. As the lactic acid bacteria, lactic acid bacteria derived from adult chickens are preferred because they are more likely to adhere to the intestinal tract wall and are suspended in tap water for administration. For the feeding of adult chickens, tap water is generally used as water for sputum, so the bacteria that are suspended in tap water for administration will not easily die, for addition to sputum water -6-.1318117 (7) Table 1 Intestinal tube fluff individual content bacteria Plexus necrosis detachment eosinophilic neutrophil macrophage lymphocytic leukocyte leukocyte 1 ++ — + ++ + + + + Example 2 + — + + + ++ + ++ 1 3 + + ++ + + + + 4 + + + + + + + 5 + — + + + + + + 1 4—h — ++ ++ + ++ + + Comparative Example 2 -H-+ — +++ +Hf + + + + 1 3 ++ — ++ +++ + + + + 4 ++ A++ ++ + + + ++ 5 +++ A +++ +++ + + + ++ • — ! Nothing slightly, + + :Multiple, + + + : The number of chickens that were killed or not recovered in the test area and the control area after 2 days from the forced moulting, the number of chickens killed in the evaluation was 201 and 282 respectively. Only (ρ<〇·〇〇1), and the number of chickens that died or were eliminated within 5 weeks, the test area was 1 〇 5, and the control area was 290 (ρ < 0.00 1). Further, the number of various bacterial plexuses in the feces, the ileum wall, and the cecum was measured from the time of fasting to the start of the feeding. The results are shown in Table 2, Table 3 and Table 4. Moreover, the sampling date in the table is not important, and the number of days is the number of days since the hunger strike. -11 - (8) .1318117 Table 2 Number of fecal neutralization bacteria (log CFU/g) Comparative Example 1 (number of individuals: 6) Example 1 (number of individuals: 6) Sampling 曰 3/29 4/17 4/24 3/29 4/17 4/24 (Re-giving (giving the feed (giving the feed (re-giving (2 days after giving the feed (before feeding the feed)) 9 days after the feed) 2 days after the feed) 9 days after the intestine Entero bacteriaceae 5.61±0.88 7.99+0.51 6_06±0.57 5.39±0.74 7.79±0.38 5_99±0.92 Clostridium perfringens 2.32 6.26 soil].]0 4.84+0.72 2.32 5.65±0.84 4.55±1.17 (C.perfringens) (1/6) (6/6) (6/6) (1/6) (6/6) (6/6) Salmonella ND ND ND ND ND ND Lactobacillus 8.67±0_37 6.74±0.80 8.72±0.34 8.80±0_19 8.65±0.19** 8.53±0.67** Total number of bacteria: 9.0010.30 9.44±0.17 9.35±0.46 8.95±0.19 9.2^0.17 9.04±0_33 Total number of bacteria in lactic acid bacteria (anaerobic) (%) 50.1117.8 0.5±0.5 31.5129.1 71.0115.0 27.5±14.3 51.4±42.5

**p<0.01, (/): number of individuals detected by bacteria / total number of assays - 12 - • 1318117 Ο) Table 3 Number of bacteria in the ileum wall (l〇gCFU/g) Comparative Example 1 (number of individuals: 5) Example 1 (number of individuals: 5) Sampling day 3/29 4/9 4/12 4/15 3/29 4/9 4/12 4/15 (front) (3曰) (6曰) (9曰) 湔) (3曰) (6曰) (9曰) Intestinal bacteria 2_82 soil 4.01 soil 4.07 soil 3.58 soil 4.12 ± 4.40 soil 4.67 soil 6.17 soil (Entero- 0.16 0.88 1.67 1.48 0.62 0.69 1.54 1.03* bacteriaceae) ( 3/5) (4/5) (4/5) (4/5) (4/5) (3/5) Clostridium perfringens ND ND ND ND ND ND ND ND (C.perfringens) Salmonella ND ND ND ND ND ND ND ND (Salmonella) lactic acid bacteria 6.94 soil 2.30 ND ND 6.70+ 3.78 soil ND ND (Lactobacillus) 0.41 (1/5) 0.46 1.38 (5/5) (5/5) (2/5) Total bacterial count 7.21 Soil 6.94 soil 5.81 ± 6.07 soil 6.93 soil 6.88 soil 6.12 soil 6.30 soil 0.44 0.30 0.95 0.56 0.56 0.52 1.01 0.94 (3/5) (3/5) Lactic acid bacteria total bacteria number 65.3 soil 0.00 7.11 ± 1.5 (anaerobic) (%) 39.3 (1/5) (0/5) (0/5) 40.1 (1/5) (0/5) (0/5) (5/5) (5/5)

P<0_01, (/): number of individuals detected by bacteria/total number of measurements-13- (10) • 1318117 Table 4 Number of bacteria in the cecal wall (log CFU/g) Comparative example 1 (number of individuals: 5) Example 1 (number of individuals: 5) Sampling 曰 3/29 4/9 4/12 4/15 3/29 4/9 4/12 4/15 (front) (3曰) (6曰) (9曰) (前) (3曰) (6曰) (9曰) Intestinal bacteria 4.63 soil 7.51 soil 7.24 soil 7.88 soil 5.16 soil 6.58 soil 7.80 ± 7.66 soil (Entero- 1.67 1.47 0.86 0.31 1.25 1.54 0.44 0.75 bacteriaceae) Clostridium perfringens ND 3.24 2.73 3.35 soil ND 3.03 ND 3.67 (C.perfringens) (1/5) (1/5) 0.45 (1/5) (1/5) (3/5) Salmonella ND ND ND ND ND ND ND ND ( Salmonella) lactic acid bacteria 8.28 soil 5.12 soil 4.02 soil 4.06 soil 8.59 soil 5.85 soil 4.83 soil 5.86 (Lactobacillus) 0.60 0.87 1.18 1.50 0.5 0.53 1.38 (1/5) (3/5) (2/5) (3/4) (3 /5) Total number of bacteria 9.01 soil 9_09 soil 9.07 soil 9.07 soil 9.41 ± 9.45 soil 9_09 soil 9.52 soil 0.71 0.27 0.39 0.23 0.25 0.12 0.19 0.18 total bacterial count 23.7 soil 0.04 soil 0.01 ± 0.01 soil 21.4 soil 0.05 soil 0_26 ± 0.02 (anaerobic) (%) 15.8 0.05 0.02 0 .01 21.0 0.04 0.43 (1/5) (3/5) (2/5) (5/5) (3/4) (3/5)

(/): The number of individuals detected by the bacteria / the total number of measurements - 14 - 13, 18117. (11) Example 2 60000 chickens (Hy-line jeria), at 6〇0〇〇 In the windowless house of the ridge, the Π 1 5 - 3 (Showa Industry Co., Ltd.) feed is given for feeding. When the chicken arrived at 4 3 5 days, it performed a 7-day hunger strike and forced the feathers. Two days before the onset of the hunger strike, Lactobacillus laurelii NHL2 and LH8 were added to the water to a concentration of 1 X 10 8 CFU per unit for 2 hours of water supply. Further, during 7 days of the hunger period, 3.5 x 105 CFU/ml of NHL2, 3.5 x 105 CFU/ml of LH8, and 56//g/ml of oligosaccharide having the composition shown in Table 5 were administered. Table 5 Ingredient name Standard content (% by mass) Moisture 1. 1 Stachyose 12.0 Raffinose 4.0 Maltose 3.5 Wheat tooth trisaccharide 4.5 Maltotetraose 25.0 Sucrose 20.0 Other sugars 29.7 Determination of feces from pre-fasting to feeding The number of various bacterial clusters in the middle, ileal wall and cecum. The results are shown in Tables 6 and 7. Moreover, the sampling date in Table -15- 1318117 (12) is not important, and the number of days is the number of days since the hunger strike. Table 6 Number of bacteria in feces (log CFU/g) Sampling day (number of individuals: 5) 9/26 湔) 10/8 (3 后 after feeding) Enteric bacteria 5.08±0.61 6.08±0.76 (Enterobacteriaceae) Clostridium 3.39+1.28 3.13±0.71 (C.perfringens) (4/5) (3/5) Salmonella ND ND (Salmonella) Lactic acid bacteria 8.79+0.42 9.00±0.13 L. lactis NHL2 ND 8.09±0.29 Lactobacillus saliva LH8 ND 8.11±0.31 Total bacterial count 9.12+0.42 9.24±0.10 Total bacterial count of lactic acid bacteria (anaerobic) (%) 47.816.5 58.9±16.1 NHL2/total lactic acid bacteria (%) — 14.6±8.3 LH8/total lactic acid bacteria (%) — 14.4± 7.4

(/): Number of individuals detected by bacteria / total number of measurements - 16 - (13) 1318117 Table 7 Number of bacteria in ileum and cecum (log CFU/g) Date of ileal cecum sampling (number of individuals: 5) 9/26 (前) 10/4(6 曰) 9/26(前前) 10/4(6 曰) Enterobacteria ND 5.15+0.64 3.60±0.05 6.40+1.14 (Enterobacteriaceae) (2/5) (4/5) Clip Clostridium ND ND ND 2.84 (C. perfringens) (1/5) Salmonella ND ND ND ND (Salmonella) Lactic acid bacteria 6.42 ± 0.55 4.12 8.79 ± 0.42 3.77 ± 0.64 (1/5) (3/5) Lactobacillus NHL2 ND ND ND ND Lactobacillus saliva LH8 ND 4.03 ND 4.23 (1/5) (1/5) Total number of bacteria 6.96 ± 0.42 6.39 ± 1.54 9.41 ± 0.20 9.59 + 0.10 (4/5) Total number of bacteria in lactic acid bacteria (anaxia) ) (%) 36_5±18.4 46.9 27.4±11.9 0.0±0.0 NHL2/total lactic acid bacteria (%) (4/5) (1/5) (3/5) LH8/total lactic acid bacteria (%) one — — — one 82.0 — 64.5 (1/5) (1/5)

(/): Number of individuals detected by bacteria / total number of measurements -17 - 1318117 (14) Reference Example 1 Various lactic acid bacteria of the lactic acid bacteria shown in Table 8 were suspended in tap water (when the suspension concentration of each example was 0 time) The concentration of the viable bacteria in the suspension was determined by a general method at room temperature. The results are shown in Table 8. Of the five strains tested, three were from L. johnsonii and L. Johns onii, which were disclosed in Japanese Laid-Open Patent Publication No. Hei. No. Hei. Examples of lactic acid bacteria in adult chickens of NHL2 and LH8.

Table 8 Hours of bacteria (pcs/ml) L.reuteri L.reuteri L.johnsonii NHL2 LH8 from chicks from chicks from chicks from adult chickens from adult chickens 0 6.4xl05 6.0x10s 1. 0x1 Ο5 9.5x1〇10 6_0xl〇】〇 1 4.2x1〇5 1.0x105 4·0χ104 One— 2 5.2x1〇4 2.2x104 3,2χ104 One—3 _ 4.4χ1010 2.2x1〇丨0 4 6.8x103 8.0x1 Ο2 7.8x103 One—8 2.0x102 0.0 1 ·0χ1 Ο3 one — 24 0.0 0.0 0.0 3.0x1〇10 2.0x10'° -18-

Claims (1)

1318117 Picking up, applying for patent coverage No. 93 1 0410 Patent application No. 1 Patent application Revision of the Chinese patent application on September 16, 1998. Amendment of a kind of defense against forced damage to feathers, characterized by containing Lactobacillus Salivarius, registration number: BCRC 9 1 025 1 ), lactic acid bacteria with Lactobacillus reuteri (registered number: BCRC 910250) as an active ingredient. 2. The agent according to claim 1, wherein the lactic acid bacteria proliferating oligosaccharide containing stachyose, raffinose, maltose, maltotriose, maltotetraose, sucrose and other sugars .