CN1533708A - Injure preventing agent for forcedly changing feather and method for forcedly changing feather - Google Patents
Injure preventing agent for forcedly changing feather and method for forcedly changing feather Download PDFInfo
- Publication number
- CN1533708A CN1533708A CNA200410030246XA CN200410030246A CN1533708A CN 1533708 A CN1533708 A CN 1533708A CN A200410030246X A CNA200410030246X A CN A200410030246XA CN 200410030246 A CN200410030246 A CN 200410030246A CN 1533708 A CN1533708 A CN 1533708A
- Authority
- CN
- China
- Prior art keywords
- lactic acid
- acid bacteria
- injury
- acidi lactici
- bacillus acidi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
To provide a forced molting method for carrying out effective forced molting without damaging poultry, to obtain a forced molting adjuvant therefor and a protective agent for protecting damages by a reduced feed and fasting in animals. The protective agent for a forced molting injury comprises lactic acid bacteria as an active ingredient. The forced molting method comprises a process for making poultry fast and a process for administering the protective agent for the forced molting injury to the poultry. The protective agent for an injury in hunger comprises lactic acid bacteria as an active ingredient. (C)2004,JPO&NCIPI.
Description
Technical field
The present invention relates to a kind of anti-injury agent, it can prevent that laying hen (layer) etc. from coming to harm because of hungry in the forced molting process.The present invention also relates to use above-mentioned anti-injury agent poultry to be carried out the method for forced molting.
Background technology
The laying hen of laying eggs is along with the ageing meeting loses egg laying performance gradually.And the egg that such chicken the gives birth to heavy cracky that both often becomes, this has just reduced its commercial value.In a period of time, stop feeding or stop feeding and thereby drinking water makes chicken stop to lay eggs, recover feeding again after waiting its feather to take off, chicken is recovered.This method is called forced molting, and is used widely in the industry of laying eggs, to improve the economic benefit that egg is produced.
In the laying hen that forced molting is handled, many individualities have shown the raising of egg laying performance and egg quality in a group, yet, also there are some individual, and may die or lose egg laying performance owing to feeding stops to be subjected to injury.This injury also can cause the protection of harmful bacteria is weakened, thereby causes the contaminated possibility of egg to increase.Therefore, need find a kind of can carry out effective forced molting, again can be to the way that damages such as poultry such as laying hens.
Known lactic acid bacteria, as Luo Shi Bacillus acidi lactici (Lactobacillus reuteri), can be applied to chicken and the propagation of salmonella is suppressed (JP-10-265395-A, JP-10-330274).But, people and do not know that lactic acid bacteria also can be and reduce the purpose that forced molting damaged and be applied to the chicken that grows up up to now.
Disclosure of the Invention
The purpose of this invention is to provide the anti-injury of a kind of forced molting agent, it can not damage poultry in the process of carrying out effective forced molting, and a kind of method of using above-mentioned anti-injury agent to carry out forced molting is provided.
For achieving the above object, the inventor has done big quantity research, the enteron aisle that discovery is handled the back poultry through forced molting has suffered the serious cellular damage that caused by hunger, and the above-mentioned damage that is caused by hunger in forced molting can significantly improve by taking a kind of anti-injury agent that contains lactic acid bacteria, thereby has finished the present invention.
According to the present invention, the anti-injury of forced molting agent is provided, described anti-injury agent contains the lactic acid bacteria as active component.
According to the present invention, a kind of method of forced molting also is provided, described method comprises the steps: to stop to the poultry feeding, allows described poultry take the anti-injury of forced molting above-mentioned agent.
The preferred embodiments of the invention
The anti-injury of forced molting of the present invention agent contains the lactic acid bacteria as active component.Described lactic acid bacteria preferably is that the form of viable bacteria exists when taking.
Lactic acid bacteria preferably comes from the laying hen that grows up, because such lactic acid bacteria can be than the intestinal walls that is easier to be attached to poultry, even when it is suspended in the running water of Gong taking, also survive easily.Because running water normally offers the chicken that grows up as drinking water, therefore,, sneaked at it and to be had superiority especially when drinking water is used even those are suspended in the lactic acid bacteria that running water also can survive.
Lactic acid bacteria is the bond of homotype (homo) lactic acid bacteria and abnormal shape (hetero) lactic acid bacteria preferably, described homotype lactic acid bacteria refers to the bacterial classification that only glucose is changed into lactic acid, and special-shaped lactic acid bacteria refers to the bacterial classification that glucose is changed into lactic acid and other material such as acetic acid, this can make lactic acid bacteria extensively be distributed in intestines, and don't can damage enteron aisle.
The homotype lactic acid bacteria can comprise Lactobacillus casei (Lactobacillus casei), lactobacillus plantarum (L.plantarum), Lactobacillus acidophilus (L.acidophilus) and saliva Bacillus acidi lactici (L.salivarius), especially preferred saliva Bacillus acidi lactici.
Betabacterium can comprise Luo Shi Bacillus acidi lactici (L.reuteri) and fermentation lactobacillus (L.fermentum), especially preferred Luo Shi Bacillus acidi lactici.
The saliva Bacillus acidi lactici is preferably saliva Bacillus acidi lactici LH8 bacterial strain and [is preserved in the biological depositary institution-Independent Administrative Leged Industrial Technology Complex Inst of international monopoly, deposit number is FERM BP-08617, on February 13rd, 2004 (identical with the bacterium that on January 8th, 2003, deposit number was FERM P-19181)].The Luo Shi Bacillus acidi lactici is preferably Luo Shi Bacillus acidi lactici NHL2 bacterial strain and [is preserved in the biological depositary institution-Independent Administrative Leged Industrial Technology Complex Inst of international monopoly, deposit number is FERM BP-08618, on February 13rd, 2004 (identical with the bacterium that on January 8th, 2003, deposit number was FERMP-19182)].
Except lactic acid bacteria, anti-injury agent of the present invention also can comprise the compound sugar that can promote lactobacter growth in the intestines.Thereby described compound sugar can be those can not digested by digestive ferment but can be utilized the compound sugar that can promote lactic acid bacteria propagation by lactic acid bacteria.Especially, described compound sugar is galactolipin compound sugar, fructose oligosaccharides, wood sugar compound sugar or its mixture.
Total lactic acid bacteria of per 1 CFU can contain the 0-150mg compound sugar in the anti-injury agent of the present invention.Like this, the compound sugar in the above-mentioned anti-injury agent helps to improve the survival ability that has adhered to and settled down the lactic acid bacteria on small bowel, thereby strengthens the effectiveness of alkaline intestinal juice of neutralization and pancreatic juice, and promotes the propagation of lactic acid bacteria, and then suppresses the harmful bacteria in the large intestine.
The content of lactic acid bacteria can be adjusted to the degree to take than suitable dose without limits aptly in the anti-injury agent of the present invention, and this will be described below.
The taking dose of anti-injury agent of the present invention can be with respect to the every kg body weight of used poultry every day 10
5CFU-5 * 10
8Total lactic acid bacteria number of CFU.When described lactic acid bacteria contained homotype and special-shaped lactic acid bacteria simultaneously, preferably the content of each was with respect to the every kg body weight of poultry every day 10
5-10
8CFU.The ratio of homotype lactic acid bacteria and special-shaped lactic acid bacteria was not limited to 1: 1.
Processing object of this anti-injury agent is poultry such as laying hen, and especially chicken age was 400 days or chicken higher, that egg laying performance descends.
Can comprise by taking the injury that causes by forced molting that anti-injury agent of the present invention avoids, during forced molting owing to the hungry intestinal walls cells injury that causes.Be not limited to existing theory, be sure of that the mechanism of protective action is as described below.
Intestinal juice of poultry and pancreatic juice are alkaline, and wherein contained major protein enzyme is trypsase and chymotrypsin, and these two kinds of protease have the suitableeest alkaline pH value.When poultry is in starvation, the protease digestion in intestinal juice and the pancreatic juice albumen of the villus cell on the intestinal walls, thereby cause the meronecrosis on the intestinal walls and come off.
In this case, when adhering to easily and settling down after the abnormal shape on small intestine and the large intestine wall and homotype lactic acid bacteria are taken, these bacteriums are attachable to intestinal walls justacrine lactic acid, thus the alkalescence of neutralized intestinal juice and pancreatic juice, so that prevent the protease digestion enterocyte.So also just prevent hungry period the intestinal walls cell necrosis and come off, thereby prevented during forced molting owing to the feeding minimizing or stop to damage.
Forced molting method of the present invention comprises the steps: to stop to take anti-injury agent of the present invention to poultry again to such as poultry feedings such as laying hens.
The time length that stops feeding having no particular limits, and can be adjusted to aptly to be fit to can well recover after the forced molting, can be 6-14 days usually.Preferably, fasting is carried out in the mode that stops feeding and continue to supply with drinking water simultaneously, but also can select to carry out for the mode of giving the feed that does not contain nutritional labeling.In the example of back, anti-injury agent of the present invention can be added in the drinking water, perhaps is added in the nonnutritive feed.
The duration of taking anti-injury agent also has no particular limits, can be before fasting, among and any one or more snippets time afterwards take.Preferably, before fasting, begin to take, and continue to take after the fasting phase finishes.For example, begin first three sky in fasting and just take, finish back three days up to the fasting phase.Can be different at the dose that stops this anti-injury agent between feeding period with the dose before stopping feeding.Whether contain compound sugar and analogous components thereof and their content, can change.
Forced molting of the present invention anti-injury agent and forced molting method make and carry out effective forced molting come true under the prerequisite that poultry is not damaged.And, also can expect to the growth inhibitory action of poultry intestinal toxic bacterium, thereby quantity, quality and the security of egg production all are improved.
Embodiment
Now the present invention is made more detailed description, but the present invention is not limited to described embodiment with embodiment.
Embodiment 1 and comparative example 1
(kind: Highline Laura) is closed in a pouity dwelling place that does not have a window, each pouity dwelling place can hold 60000 chickens and be equipped with two independently water supply pipes, 30000 chickens of every water pipe confession for 60000 laying hens.J115-3 (being produced by SHOWA SANGYO Co., Ltd) is used as feed and supplies.Reach that day of 447 days age from chicken, chicken is stopped feeding 9 days to carry out forced molting.
Wherein the water supply pipe of 30000 chickens of a confession is as embodiment 1.Begin a few days ago stopping feeding, Luo Shi Bacillus acidi lactici NHL2 bacterial strain (FERM BP-08618) (hereinafter referred to as NHL2) and saliva Bacillus acidi lactici LH8 bacterial strain (FERM BP-08617) (hereinafter referred to as LH8) are supplied with chicken in the mode that is contained in the drinking water, and the concentration of every kind of bacterium is 1 * 10
8The water that every chicken of CFU/ is every two hours supplied.During 9 days fasting, the concentration of NHL2 and every kind of bacterium of LH8 is 3.5 * 10 in institute's supplying drinking water
5CFU/ml.After fasting finishes first day supplies every two hours that the concentration of NHL2 and every kind of bacterium of LH8 is 1 * 10 in the drinking water
8CFU/ml.Another root in two water pipes is for the water pipes of other 30000 chickens embodiment 1 as a comparison, and the drinking water by this root water pipe is a water.
In the last day of fasting phase, from embodiment 1 and comparative example 1, respectively slaughter 5 chickens.Detect the composition in the ileum, thereby measure the quantity of exfoliate stomach tissue, the state to flora and terminal ileum villus cell carries out histochemistry's mensuration simultaneously.The results are shown in Table 1
Table 1
Enteron aisle | Enteron aisle fine hair | ||||||||
Sample number | Content | Flora | Downright bad | Peel off | Eosinocyte | Neutrophil leucocyte | Macrophage | Lymphocyte | |
Embodiment 1 | ????1 | ????++ | ????- | ????+ | ????++ | ????+ | ????+ | ????+ | ????+ |
????2 | ????+ | ????- | ????+ | ????+ | ????+ | ????++ | ????+ | ????++ | |
????3 | ????++ | ????+ | ????+ | ????++ | ????+ | ????+ | ????+ | ????+ | |
????4 | ????+ | ????+ | ????+ | ????+ | ????+ | ????+ | ????+ | ????+ | |
????5 | ????+ | ????- | ????+ | ????+ | ????+ | ????+ | ????+ | ????+ | |
The comparative example 1 | ????1 | ????++ | ????- | ????++ | ????++ | ????+ | ????++ | ????+ | ????+ |
????2 | ????+++ | ????- | ????+++ | ????+++ | ????+ | ????+ | ????+ | ????+ | |
????3 | ????++ | ????- | ????++ | ????+++ | ????+ | ????+ | ????+ | ????+ | |
????4 | ????++ | ????- | ????++ | ????++ | ????+ | ????+ | ????+ | ????++ | |
????5 | ????+++ | ????- | ????+++ | ????+++ | ????+ | ????+ | ????+ | ????++ |
-: do not measure; +: trace; ++: a large amount of; +++: is very a large amount of
In the test in time fortnight that forced molting begins, because it is dead or do not have the possibility that can revert to laying hen and the number of slaughtered chicken, be respectively 201 and 282 (p<0.001) in test group and control group, these numerals are not included as testing goal and the chicken slaughtered.The number of dead or slaughtered chicken is 105 in test group, and is 290 (P<0.001) in control group in subsequent five week.
From before the beginning fasting after recover feeding, to ight soil, the number of each flora is measured in ileum wall and the caecum wall.The result sees Table 2,3,4 respectively.In these tables, unless otherwise indicated, the numerical value on date refers to from the fate of beginning fasting calculating in this hurdle of Date of Sampling.
Table 2: flora number in the ight soil (log CFU/g)
Comparative example 1 (6 samples) | Embodiment 1 (6 samples) | |||||
Date of Sampling (M/D) | 3/29 (before recovering feeding) | 4/17 (recovering feeding after 2 days) | 4/24 (recovering feeding after 9 days) | 3/29 (before recovering feeding) | 4/17 (recovering feeding after 2 days) | 4/24 (recovering feeding after 9 days) |
Enterobacteria | ????5.61±0.88 | ????7.99±0.51 | ????6.06±0.57 | ????5.39±0.74 | ????7.79±0.38 | ????5.99±0.92 |
C.perfringens | ????2.32(1/6) | ????626±1.10(6/6) | ????4.84±0.72(6/6) | ????2.32(1/6) | ????5.65±0.84(6/6) | ????4.55±1.17(6/6) |
Salmonella | ????ND | ????ND | ????ND | ????ND | ????ND | ????ND |
Bacillus acidi lactici | ????8.67±0.37 | ????6.74±0.80 | ????8.72±0.34 | ????8.80±0.19 | ????8.65±0.19 ** | ????8.53±0.67 ** |
Bacteria total amount | ????9.00±0.30 | ????9.44±0.17 | ????9.35±0.46 | ????8.95±0.19 | ????9.27±0.17 | ????9.04±0.33 |
Bacillus acidi lactici/bacteria total amount (anaerobic) (%) | ????50.1±17.8 | ????0.5±0.5 | ????31.5±29.1 | ????71.0±15.0 | ????27.5±14.3 | ????51.4±42.5 |
**p<0.01
(/): be detected the number of samples of bacterium/be examined the altogether total number of sample
Table 3: the number of flora on the ileum wall (log CFU/g)
Comparative example 1 (5 samples) | Embodiment 1 (5 samples) | |||||||
Date of Sampling (M/D) | 3/29 (in the past) | 4/9 (3 days) | 4/12 (6 days) | 4/15 (9 days) | 3/29 (in the past) | 4/9 (3 days) | 4/12 (6 days) | 4/15 (9 days) |
Enterobacteria | ??2.82±0.16 ??(3/5) | ????4.01±0.88 ????(4/5) | ????4.07±1.67 | ????3.58±1.48 | ????4.12±0.62 ????(4/5) | ????4.40±0.69 ????(4/5) | ????4.67±1.54 ????(4/5) | ????6.17±1.03 * |
C.perfringens | ??ND | ????ND | ????ND | ????ND | ????ND | ????ND | ????ND | ????ND |
Salmonella | ??ND | ????ND | ????ND | ????ND | ????ND | ????ND | ????ND | ????ND |
Bacillus acidi lactici | ??6.94±0.41 ??(5/5) | ????2.30(1/5) | ????ND | ????ND | ????6.70±0.46 ????(5/5) | ????3.78±1.38 ????(2/5) | ????ND | ????ND |
Bacteria total amount | ??7.21±0.44 | ????6.94±0.30 ????(3/5) | ????5.81±0.95 | ????6.07±0.56 | ????6.93±0.56 | ????6.88±0.52 ????(3/5) | ????6.12±1.01 | ????6.30±0.94 |
Bacillus acidi lactici/bacteria total amount (anaerobic) (%) | ??65.3±39.3 ??(5/5) | ????0.00 ????(1/5) | ????(0/5) | ????(0/5) | ????71.1±40.1 ????(5/5) | ????1.5 ????(1/5) | ????(0/5) | ????(0/5) |
*p<0.05
(/): be detected the number of samples of bacterium/be examined the altogether total number of sample
Table 4: the number of flora on the caecum wall (log CFU/g)
Comparative example 1 (5 samples) | Embodiment 1 (5 samples) | |||||||
Date of Sampling (M/D) | 3/29 (in the past) | 4/9 (3 days) | 4/12 (6 days) | 4/15 (9 days) | 3/29 (in the past) | 4/9 (3 days) | 4/12 (6 days) | 4/15 (9 days) |
Enterobacteria | ??4.63±1.67 | ??7.51±1.47 | ??7.24±0.86 | ??7.88±0.31 | ??5.16±1.25 | ??6.58±1.54 | ????7.80±0.44 | ????7.66±0.75 |
C.perfringens | ??ND | ??3.24(1/5) | ??2.73(1/5) | ??3.35±0.45 ??(3/5) | ??ND | ??3.03(1/5) | ????ND | ????3.67(1/5) |
Salmonella | ??ND | ??ND | ??ND | ??ND | ??ND | ??ND | ????ND | ????ND |
Bacillus acidi lactici | ??8.28±0.60 | ??5.12±0.87 | ??4.02±1.18 ??(3/5) | ??4.06±1.50 ??(2/5) | ??8.59±0.5 | ??5.85±0.53 ??(3/4) | ????4.83±1.38 ????(3/5) | ????5.86(1/5) |
Bacteria total amount | ??9.01±0.71 | ??9.09±0.27 | ??9.07±0.39 | ??9.07±0.23 | ??9.41±0.25 | ??9.45±0.12 | ????9.09±0.19 | ????9.52±0.18 |
Bacillus acidi lactici/bacteria total amount (anaerobic) (%) | ??23.7±15.8 | ??0.04±0.05 | ??0.01±0.02 ??(3/5) | ??0.01±0.01 ??(2/5) | ??21.4±21.09 ??(5/5) | ??0.05±0.04 ??(3/4) | ????0.26±0.43 ????(3/5) | ????0.02 ????(1/5) |
(/): be detected the number of samples of bacterium/be examined the altogether total number of sample
Embodiment 2
(kind: Highline Julia) closed in a pouity dwelling place that does not have a window, each pouity dwelling place can hold 60000 chickens for 60000 laying hens.J115-3 (being produced by SHOWA SANGYO Co., Ltd) is used as feed supply.Reach that day of 435 days age from chicken, chicken is stopped feeding 7 days to carry out forced molting.
Begin a few days ago in fasting, NHL2 bacterial strain and LH8 bacterial strain are supplied with chicken in the mode that is contained in the drinking water, and the concentration of every kind of bacterium is 1 * 10
8The water that every chicken of CFU/ is every two hours supplied, during this fasting of 7 days, 3.5 * 10
5The NHL2 of CFU/ml and 3.5 * 10
5The compound sugar of the LH8 of CFU/ml and 56 micrograms/ml is supplied with in the mode of drinking water, and described compound sugar is a kind of composition, and its composition sees Table 5.
Table 5
Component | Standard content (wt%) |
Water | ????1.1 |
Stachyose | ????12.0 |
Gossypose | ????4.0 |
Maltose | ????3.5 |
Maltotriose | ????4.5 |
Maltotetraose | ????25.0 |
Sucrose | ????20.0 |
Other sugar | ????29.7 |
From before the beginning fasting after recover feeding, to ight soil, the number of each flora is measured in ileum wall and the caecum wall.The result sees Table 6,7 respectively.In these tables, unless otherwise indicated, the numerical value on the date in this hurdle of Date of Sampling refers to from the fate of fasting calculating.
Table 6: the number of flora in the ight soil (log CFU/g)
Date of Sampling (M/D) (5 samples) | 9/26 (in the past) | 10/8 (behind the feeding 3 days) |
Enterobacteria | ????5.08±0.61 | ????6.08±0.76 |
C.perfringens | ????3.39±1.28(4/5) | ????3.13±0.71(3/5) |
Salmonella | ????ND | ????ND |
Bacillus acidi lactici Luo Shi Bacillus acidi lactici NHL2 saliva Bacillus acidi lactici LH8 | ????8.79±0.42 ????ND ????ND | ????9.00±0.13 ????8.09±0.29 ????8.11±0.31 |
Bacteria total amount | ????9.12±0.42 | ????9.24±0.10 |
Bacillus acidi lactici/bacteria total amount (anaerobic) is NHL2/ Bacillus acidi lactici total amount (%) LH8/ Bacillus acidi lactici total amount (%) (%) | ????47.8±6.5 ????- ????- | ????58.9±16.1 ????14.6±8.3 ????14.4±7.4 |
Table 7: the number of flora in ileum and the caecum (log CFU/g)
Ileum | Caecum | |||
Date of Sampling (M/D) (5 samples) | 9/26 (in the past) | 10/4 (6 days) | 9/26 (in the past) | 10/4 (6 days) |
Enterobacteria | ????ND | ????5.15±0.64(2/5) | ????3.60±0.05(4/5) | ????6.40±1.14 |
C.perfringens | ????ND | ????ND | ????ND | ????2.84(1/5) |
Salmonella | ????ND | ????ND | ????ND | ????ND |
Bacillus acidi lactici Luo Shi Bacillus acidi lactici NHL2 saliva Bacillus acidi lactici LH8 | ????6.42±0.55 ????ND ????ND | ????4.12(1/5) ????ND ????4.03(1/5) | ????8.79±0.42 ????ND ????ND | ????3.77±0.64(3/5) ????ND ????4.23(1/5) |
Bacteria total amount | ????6.96±0.42(4/5) | ????6.39±1.54 | ????9.41±0.20 | ????9.59±0.10 |
Bacillus acidi lactici/bacteria total amount (anaerobic) is NHL2/ Bacillus acidi lactici total amount (%) LH8/ Bacillus acidi lactici total amount (%) (%) | ????36.5±18.4(4/5) ????- ????- | ????46.9(1/5) ????- ????82.0(1/5) | ????27.4±11.9 ????- ????- | ????0.0±0.0(3/5) ????- ????64.5(1/5) |
(/): be detected the number of samples of bacterium/be examined the altogether number of sample
With reference to embodiment 1
Each lactic acid bacteria strains in the Lactobacillus in the table 8 all is suspended in (concentration of every kind of suspension is 0 hour concentration) in the running water.Suspension remains under the room temperature state, and measures the number of viable bacteria in the suspension frequently with conventional method.The results are shown in Table 8.In five determined bacterial strains, two is Luo Shi Bacillus acidi lactici (L.reuteri), one of them is Johnson's Bacillus acidi lactici (L.johnsonii), these bacterial strains all derive from chicken, and open in JP-10-265395-A and JP-10-330274-A, two remaining bacterial strains are the NHL2 and the LH8 lactic acid bacteria strains that derive from adult chicken used among each embodiment.
Table 8
Bacterium number (cells/ml) | |||||
Hour | Derive from the Luo Shi Bacillus acidi lactici of chicken | Derive from the Luo Shi Bacillus acidi lactici of chicken | Derive from Johnson's Bacillus acidi lactici of chicken | Derive from the Luo Shi Bacillus acidi lactici of the chicken that grows up | Derive from the saliva Bacillus acidi lactici of chicken |
????0 | ????6.4×10 5 | ????6.0×10 5 | ????1.0×10 5 | ????9.5×10 10 | ????6.0×10 10 |
????1 | ????4.2×10 5 | ????1.0×10 5 | ????4.0×10 4 | ????- | ????- |
????2 | ????5.2×10 4 | ????2.2×10 4 | ????3.2×10 4 | ????- | ????- |
????3 | ????- | ????- | ????- | ????4.4×10 10 | ????2.2×10 10 |
????4 | ????6.8×10 3 | ????8.0×10 2 | ????7.8×10 3 | ????- | ????- |
????8 | ????2.0×10 2 | ????0.0 | ????1.0×10 3 | ????- | ????- |
????24 | ????0.0 | ????0.0 | ????0.0 | ????3.0×10 10 | ????2.0×10 10 |
Claims (7)
1. the anti-injury of forced molting agent, it contains the lactic acid bacteria as active component.
2. anti-injury agent according to claim 1, wherein said originating in lactic acid bacterium is in adult laying hen.
3. anti-injury agent according to claim 1 and 2, wherein said lactic acid bacteria comprises homotype lactic acid bacteria and special-shaped lactic acid bacteria.
4. anti-injury agent according to claim 3, wherein said homotype lactic acid bacteria is the saliva Bacillus acidi lactici, described special-shaped lactic acid bacteria is the Luo Shi Bacillus acidi lactici.
5. anti-injury agent according to claim 4, wherein said saliva Bacillus acidi lactici are saliva Bacillus acidi lactici LH8 bacterial strain (FERM BP-08617), and described Luo Shi Bacillus acidi lactici is a Luo Shi Bacillus acidi lactici NHL2 bacterial strain (FERM BP-08618).
6. anti-injury agent according to claim 1 wherein also contains the compound sugar that can promote lactobacter growth in the intestines.
7. the method for a forced molting, it may further comprise the steps: poultry is stopped feeding, take the anti-injury of the described forced molting of claim 1 agent for above-mentioned poultry.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2003041557A JP2004250365A (en) | 2003-02-19 | 2003-02-19 | Protective agent for forced molting injury, forced molting method and protective agent against injury in hunger |
JP41557/03 | 2003-02-19 | ||
JP41557/2003 | 2003-02-19 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1533708A true CN1533708A (en) | 2004-10-06 |
CN100482095C CN100482095C (en) | 2009-04-29 |
Family
ID=33025106
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB200410030246XA Expired - Fee Related CN100482095C (en) | 2003-02-19 | 2004-02-19 | Injure preventing agent for forcedly changing feather and method for forcedly changing feather |
Country Status (4)
Country | Link |
---|---|
JP (1) | JP2004250365A (en) |
CN (1) | CN100482095C (en) |
BR (1) | BRPI0400562B1 (en) |
TW (1) | TWI318117B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007236238A (en) * | 2006-03-07 | 2007-09-20 | Bio Tec Japan:Kk | Bread and method for producing bread |
JP2012180304A (en) * | 2011-03-01 | 2012-09-20 | Biseibutsu Kagaku Kenkyusho:Kk | Drink for controlling campylobacter infection of chicken |
-
2003
- 2003-02-19 JP JP2003041557A patent/JP2004250365A/en active Pending
-
2004
- 2004-02-18 BR BRPI0400562-7A patent/BRPI0400562B1/en not_active IP Right Cessation
- 2004-02-19 TW TW093104101A patent/TWI318117B/en not_active IP Right Cessation
- 2004-02-19 CN CNB200410030246XA patent/CN100482095C/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
TWI318117B (en) | 2009-12-11 |
BRPI0400562B1 (en) | 2014-07-08 |
JP2004250365A (en) | 2004-09-09 |
BRPI0400562A (en) | 2004-12-07 |
TW200501972A (en) | 2005-01-16 |
CN100482095C (en) | 2009-04-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Bergh | Bacteria associated with early life stages of halibut, Hippoglossus hippoglossus L., inhibit growth of a pathogenic Vibrio sp. | |
Mohammadian et al. | Synbiotic effects of β-glucan, mannan oligosaccharide and Lactobacillus casei on growth performance, intestine enzymes activities, immune-hematological parameters and immune-related gene expression in common carp, Cyprinus carpio: An experimental infection with Aeromonas hydrophila | |
Munir et al. | Dietary prebiotics and probiotics influence growth performance, nutrient digestibility and the expression of immune regulatory genes in snakehead (Channa striata) fingerlings | |
Mead et al. | Some observations on the caecal micro‐flora of the chick during the first two weeks of life | |
Taoka et al. | Use of live and dead probiotic cells in tilapia Oreochromis niloticus | |
McIntosh et al. | Culture-independent characterization of the bacterial populations associated with cod (Gadus morhua L.) and live feed at an experimental hatchery facility using denaturing gradient gel electrophoresis | |
Mahdhi et al. | Probiotic properties of Brevibacillus brevis and its influence on sea bass (Dicentrarchus labrax) larval rearing | |
Nisbet et al. | Maintenance of the biological efficacy in chicks of a cecal competitive-exclusion culture against Salmonella by continuous-flow fermentation | |
SK76596A3 (en) | Agent for inhibition of pets colonization with salmonella, a process for the production and use thereof | |
Yang et al. | Probiotic Psychrobacter sp. improved the autochthonous microbial diversity along the gastrointestinal tract of grouper Epinephelus coioides | |
KR101781211B1 (en) | A composition comprised of novel Lactobacillus salivarius KCTC18458P or the culture medium thereof | |
EP3478082A1 (en) | Composition for treatment and/or nutrition of poultry | |
CA2987013C (en) | Probiotic or prebiotic, method for producing same, microbial preparation, health food, and medicine | |
BEDBURY et al. | Cecal microflora of turkeys fed low or high fiber diets: enumeration, identification, and determination of cellulolytic activity | |
Salas-Leiva et al. | Characterization of the intestinal microbiota of wild-caught and farmed fine flounder (Paralichthys adspersus) | |
EP0381700A1 (en) | Method for inhibiting the growth of salmonella. | |
Panase et al. | Influences of Bacillus subtilis and fructooligosaccharide on growth performances, immune responses, and disease resistance of Nile tilapia, Oreochromis niloticus | |
Dutta et al. | Susceptibility of fecal streptococci of poultry origin to nine growth-promoting agents | |
WO1993003742A1 (en) | ANTIBIOTIC RESISTANT STRAIN OF $i(LACTOBACILLUS ACIDOPHILUS) | |
CN1533708A (en) | Injure preventing agent for forcedly changing feather and method for forcedly changing feather | |
Tarnecki et al. | Microbiota of common snook Centropomus undecimalis larvae exhibiting high mortality. | |
Li et al. | Clostridium butyricum improves the digestive enzyme activity, antioxidant and immunity related genes expression and intestinal microbiota of Litopenaeus vannamei fed a replacing fishmeal with cottonseed protein concentrate (CPC) diet | |
WO2012067341A1 (en) | Novel probiotic bacillus sp. strain | |
EP0946108B1 (en) | A method for producing a food additive, food additive, and the use of it | |
US20240000865A1 (en) | Bacillus subtilis strain with strong inhibition of enteropathogenic and foodborne pathogenic bacteria |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20160330 Address after: Tokyo, Japan Patentee after: ASAHI CALPIS WELLNESS CO., LTD. Address before: Tokyo, Japan, Japan Patentee before: Calpis Co., Ltd. |
|
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090429 Termination date: 20180219 |
|
CF01 | Termination of patent right due to non-payment of annual fee |