1287534 九、發明說明： 【發明所屬之技術領域】 本發明係關於一種具有快速分解纖維質類有機資材之能力 之木徽菌株（sp·)。 【先前技術】 在作物永續栽培過程中使用生物製劑為現今栽培的新趨 勢，生物製劑使用的種類包含有幾大項，（1)促進植物生長，如菌 根菌、固氮菌、根圈有益微生物群。（2)病蟲害生物防治，如蘇力 菌、木黴菌、枯草桿菌。（3)改善作物生長環境，如溶磷菌。其功 用主要在促進植物生長、增加產量、減少病蟲害，其它功能尚包 含有產生植物賀爾蒙、誘發植物抗病反應、降低土壤酸化、減低 土壤鹽類累積、誘使其它有益微生物產生，這些生物製劑近年來 已有相當多的研究人員投入。生物製劑的定義如以廣泛的生物種 類區分，則其除包含作物本身外，尚包含與作物發生反應之各種 生物。如僅以微生物的製劑而言，則生物製劑只包含微生物體或 其相關代謝產物。微生物製劑使用在作物生長上的效益可粗分為 直接利益及間接利益二大類。直接利益微生物包括與亞科共生根 瘤菌（Rhizobium )，與森林作物共生之放線菌（Frankia )，促進 植物生長之固氮細菌、囊叢枝菌根菌（vesicualar-arbuscular mycorrhizal fungi)及外生菌根菌（ectomycorrhizal fungi)等五大 類，皆以能直接促進植物營養吸收方式促進生長；而間接利益微 生物則包括對植物病原菌、植物蟲害及雜草競爭之生物控制作 用，所以是屬於降低影響作物生長不良環境因子作用的間接機 制。無論其所採取的機制為何，這些微生物在實驗過程中均能顯 現促進作物生長潛能，能取代無機肥料及化學殺蟲劑，降低生產 1287534 成本及減少環境污染，、，& 資源遭受威脅的今天，物目前在各項農業 一般農業廢棄物均兼夏、_ ?九"〜、研發可能是一條明智之途。 理，將能轉化為農h、賴性，如抑微生物妥為處 源⑷。因此將農業廢 的自然法則，Μ也是，不僅合乎資源再利用 向之-。 h㈣如此龐大量有機廢棄物之重要方 微生物在堆肥化過 要角色。不同的堆積枒：二擔任有機物分解與堆肥 穩定化之重 堆肥潑酵。為達到最有==接種適當的微生物菌種，可以加逮 物菌種外，在堆積封：;=堆肥化作用，除了添加適當的微生 使微生物充分的活動，維持微生物最適宜之生長條件， -般堆肥化微生物菌種=祐亦能加強堆肥材料的發酵與分解。 肥化條件，於木屑、豆=，7以採用自然堆肥法，即依照堆 水份含量及通氣性等，===;當的材料大小、 于堆肥材料中，最後在腐:… 然界中微生物滋生 物菌種’故可做為下—抵堆肥製作時之微生物;== 堆肥材料發酵分解效率，針對不同有機物材料特性，施予、商2 微生物_ ’將是堆肥製作過程之重要步驟之_。 用微生物菌種的關鍵機制，應包括有筛選出適當的微利 &«㈣_$„_法與應驗堆肥材料中m 法等。 木黴菌（7Wd〇办rma sp.)在國外的研究上發現其除可拮抗 一些植物病原微生物如猝倒病菌sp)及立'枯絲 (施f減少作物死亡外’部份_尚可跟作= 根系共生JU«境環境’達聽護㈣、增進養份吸收及促進作 1287534 物生長、提高產量等效果，部份學者尚利用基因轉殖技術將抗病 基因導入植物體内而達到抗病功能。由於此菌有諸多優點，目前 已有國外的研究人員將此菌開發成生物防治用的生物製劑，常用 的議取以 T.virens、T.harzianum、T.atroviride 反 T.asperellum 為 ί， 應用的作物則以蔬菜、果樹、花卉及草皮為主。 本發明所篩選分離出具強纖維質類資材分解能力之木黴菌 株（sp· TCT111)，具有快速分解含稻殼之大宗有機 廢棄物之功能，可供稻殼堆肥醱酵添加使用。 【發明内容】 本發明係針對稻殼之理化特性，選出纖維性資材分解能力強 之木黴菌株（7>ie sp )，該菌株可於堆肥反應初期快速佔 正们資材以使含稻殼之資材在堆肥化過程中，能夠迅速充分 分解醱酵及腐熟。 菸醅本目的係提供一種能將含稻殼之有機堆肥材料分解 :-之黴菌株，該菌株之特徵係為：2〇。〇時於 ％ 萃取 快速二熟時呈暗綠色;氣生菌絲為白色;產 枝為二 =分生孢子柄寬度4〜4.5 一細長而微蠻；分 # ，分枝與瓶梗大部分對S，部分三個輪S;頂端瓶 8〜13〜J輪生；瓶梗彎曲’形狀呈安瓶形至近錐形，大小為 #面Γ/^33μηι;分生孢子呈球形或短觀形，表面平滑，部分 3^4，TO8.3,,m； 12〇c,6 本發明之另一目的係提供— 月27曰寄存於中華民國食品工 種木黴菌株，其係於民國93年5 業發展研究所菌種中心，其寄存 1287534 編號為BCRC 9_71之木黴菌株，或衍生自該木徽菌株的 體。 本發明之木黴菌株對稻殼堆肥材料配方具高分解能力，且可 以加速堆肥腐熟，減少堆肥化時程，降低堆肥製作成本。 【實施方式】 本發明的木黴菌株可分離自天然來源，例如：由土壌、作物 根系及各種稻殼堆肥成品，以2%水瓊脂（Water agar，W.A·)洋 茶平板法進行微生物分離，由土壤分離者稱取1克土壤置於1〇 毫升無菌水中經振盪後，取過濾液進行平板劃線分離，作物根系 分離者採根段分離法，將根段洗淨後切取〇·5公分小段後取5小 段置於W.A·中，各堆肥資材先細分成〇·5公分小段後取5小段置 於W.A·中，以上每種類資材5個培養皿，各處理於28它培養箱 中不照光培養三天後，挑取片段菌絲尖端移到馬鈴薯葡萄糖培養 基上’培養7天後觀察各菌絲生長形態，由其中挑取木黴菌菌絲 形態之菌株，進行二次純系分離後切取〇·5 cm2之菌絲塊置於裝5 cc無菌水之螺旋試管中保存。各分離菌株以pDA培養7天後，切 取菌絲塊，置入裝10克稻穀培養基之試管中培養，觀察各菌株 在其中菌絲纏繞及生長情形，並觀察其產孢情形，測定形態學性 狀，挑取能快速纏繞稻穀培養基及產孢的菌株。 經挑選出適用於稻殼堆肥的菌株經菌種中心鑑定係為 Trichoderma asperellum ( TCT111 ) Samules，Lieckfelds & Nirenberg，其菌落形態係為：20°C時於MA2 (含2%麥芽萃取物 之培養基）中生長快速（參考第一圖及第二圖），培養生菌絲白 色稀疏，產孢區域（conidiation)呈苞狀突起（pustulate )(參考第 三圖），產孢慢（5天才剛開始形成）。在顯微構造上：分生孢子 1287534 柄（conidiophore )寬度4〜4.5//m，細長而些微彎曲，分枝為重 複分枝（repeatedly branched )(參考第四圖、第五圖及第六圖）， 分枝與瓶梗（phialide)大部分對生，部份三個輪生，頂端瓶梗 2〜5個輪生；瓶梗彎曲，安瓶形（ampulliform )到近錐形 (subulate )，大小8〜13x 3〜3.3 a m ;分生孢子（conidia )近球形 或短橢圓形，大部分為平滑表面，少部分表面具不明顯疣狀，大 小3.2〜4·0χ 2.8〜3.5//m (參考第七圖及第八圖）。 本發明之木黴菌株可以是衍生自分離於天然來源之木黴菌 株的變異體，例如：這些菌株可以是通過UV照射、甲磺酸乙酯 處理、亞硝酸處理等得到的變異體。也可以是利用細胞融合或重 組DNA技術經基因工程改造產生的重組菌株。 以下實施例係用於進一步了解本發明之優點，並非用於限制 本發明之申請專利範圍。 實施例1·木徽菌株（7>/c/r沒心sp·，TCT111 )生長條件測試 及菌株特性 木黴菌（sp·，TCT111 )原菌種生長溫度在12〜36 °C，產孢適溫在24〜28°C，生長酸鹼值在5〜8，最適值在6·5〜7.5， 而利用此菌株培養於馬鈐薯葡萄糖洋菜平板培養基，於培養七天 後，以無菌水製成抱子懸浮液lx 1〇3 spore/ml，加入稻穀培養基 中培養兩〜三週，稻穀培養基係以未去殼之水稻穀粒以體積比3 : 1之比例加水後以120°C高壓滅菌40分鐘兩次，於木黴菌菌絲長 滿水稻穀粒後開使形成綠色孢子此即為量產之種菌，種菌培養成 熟時取出與消毒完全之稻穀培養基以三明治夾層培養法量產接 1287534 種菌種，三明治夾層培養法係以種菌+消毒完全之稻穀+種菌方 式培養，以此法進行接種源之後發酵，稻穀培養基後發酵成固體 菌種其孢子濃度可達lx 1〇12 spor*e/g，生產時間可在二十天内完 成，比其它菌株快10天以上。稻穀培養基所製備之固體菌種在 室溫下儲存活性可維持一年以上，如以低溫（-4°C或-30°C)儲存則 時間可更長。 實施例2.使用木黴菌製作堆肥的溫度效應 稻殼堆肥材料之各成分乾物重用量比例依序為稻殼 55-65%、米糠15-25%及油粕15-25%。將堆肥材料混合均勻後再 取用適量木黴菌TCT111，菌數約lx 1012 spore/ml，先加水稀釋 200倍成菌懸液，將菌稀釋液混入堆肥材料中，最後將堆肥材料 水份含量調整至60%，堆積高度維持約1.5〜2.0m，爾後立即進行 堆積製作，堆肥化期間約5〜7曰利用鏟裝機翻堆乙次，一直持續 到堆肥腐熟為止。下表一係為堆肥化過程的溫度效應，CK係為 未接種木黴菌之對照組，在有接種木黴菌的稻殼堆肥製作堆積第 2〜3曰，堆肥體溫度上升到60°C以上，最高可達到70°C，此高溫 期（>60°C)約維持10〜13曰，爾後溫度逐漸降低，約第31〜35曰， 堆肥溫度可降低至約46°C左右，約第36〜45日，堆肥溫度可降低 至約42°C左右。未接種木黴菌之稻殼堆肥，在堆肥製作堆積第 4〜6曰，堆肥體溫度上升到60°C以上，最高可達到67°C，約第 31〜35日，堆肥溫度可降低至約50°C左右，約第36〜45日，堆肥 溫度才降低至約41°C左右。顯示接種木黴菌處理之稻殼堆肥可以 提早5〜7日達到腐熟階段。 表一、接種木黴菌（TCT111)對稻殼堆肥溫度之影響 1287534 曰數 處理 0〜5 6〜10 11 〜15 16 〜20 21 〜25 26 〜30 31 〜35 36 〜45 接種TCT111 62 70 66 57 54 50 46 42 CK 60 67 65 55 56 52 50 44 單位（°c ) 實施例3·使用木黴菌對稻殼堆肥養分含量之影響 堆肥製作同實施例2.所述，腐熟之稻殼堆肥之養分含量分析 結果顯示下表二，（TCT111)接種之生物性稻殼堆肥氮含量約 1.63%、構含量約0.44%、卸含量約1.16°/。、鈣含量約1.89%、鎮 含量約0.88%、有機質含量約67·4%、鋅含量約55ppm、銅含量 約17ppm。未接菌之稻殼堆肥氮含量約1.56%、麟含量約0.38%、 鉀含量約1.13%、鈣含量約1.92%、鎂含量約0.87%、有機質含量 約68.1%、鋅含量約49ppm、銅含量約16ppm。顯然有接種木黴 菌之稻殼堆肥的氮、磷、鉀、鋅及銅等含量均略高於未接菌處理。 有接種木黴菌（TCT111)菌種於堆肥製作過程中，以及稻殼堆肥 等製成品中，均可分離出所添加之菌種，分離率約為lx 1〇4至1 X 105spore/g，顯示所添加之木黴菌菌種可在堆肥化過程及堆肥成 品中存活。 表二、接種木黴菌（TCT111)對稻殼堆肥養分含量之影響 養分 氮 磷 鉀 鈣 鎮 有機質 鋅 銅 處理 (%) mg/kg 接種TCT111 1.63 0.44 1.16 1.89 0.88 67.4 55 17 CK 1.56 0.38 1.13 1.92 0.87 68.1 49 16 綜合上述，本發明筛選出之木黴菌株對稻殼堆肥材料配方具 1287534 高分解能力，且可以加速堆肥腐熟，減少堆肥化時程，降低堆肥 製作成本，具有相當顯著的經濟效益。 其他實施態樣 本發明之實施方法已詳述於前述實施例中，任何熟悉本技術 領域之人士皆可依本發明之說明，在不背離本發明之精神與範圍 内視需要更動、修飾本發明，因此，其他實施態樣亦包含在本發 明之申請專利範圍中。1287534 IX. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a strain of wood emblem (sp·) having the ability to rapidly decompose fibrous organic materials. [Prior Art] The use of biological agents in the continuous cultivation of crops is a new trend in today's cultivation. The types of biological agents used include several major items. (1) Promoting plant growth, such as mycorrhizal fungi, nitrogen-fixing bacteria, and root ring beneficial. Microbiota. (2) Biological control of pests and diseases, such as sclerotium, trichoderma, and Bacillus subtilis. (3) Improve crop growth environment, such as phosphate solubilizing bacteria. Its function is mainly to promote plant growth, increase yield, reduce pests and diseases, and other functions include plant hormone production, induce plant disease resistance, reduce soil acidification, reduce soil salt accumulation, and induce other beneficial microorganisms. Formulations have been put into considerable research by researchers in recent years. The definition of a biological agent, if distinguished by a wide range of biological species, includes, in addition to the crop itself, various organisms that react with the crop. If only in the case of a microbial preparation, the biological preparation contains only the microorganism or its associated metabolite. The benefits of using microbial preparations on crop growth can be broadly divided into two categories: direct benefits and indirect benefits. Direct interest microbes include Rhizobium with subfamily Rhizobium, Frankia, which is symbiotic with forest crops, nitrogen-fixing bacteria that promote plant growth, vesicualar-arbuscular mycorrhizal fungi, and ectomycorrhizal fungi The five major types of bacteria (ectomycorrhizal fungi) promote growth by directly promoting plant nutrient absorption; while indirect microbes include biological control of plant pathogens, plant pests and weed competition, so it is a reduction in adverse growth of crops. An indirect mechanism of the role of environmental factors. Regardless of the mechanism adopted, these microorganisms can show the potential for promoting crop growth during the experiment, can replace inorganic fertilizers and chemical pesticides, reduce the cost of producing 1287534 and reduce environmental pollution, and the resources are threatened today. At present, the agricultural wastes in various agricultural fields are both summer and _ _ _, R & D may be a wise way. Rationally, it will be able to be transformed into a farmer's h, and if it is a microbial source (4). Therefore, the natural law of agricultural waste is also, not only, it is compatible with the recycling of resources. h (d) The importance of such a large amount of organic waste Microorganisms have a role in composting. Different piles of mash: two as the heavy decomposition of organic matter and composting. In order to achieve the most suitable == inoculation of appropriate microbial strains, it is possible to add bacteria to the bacteria, in the accumulation of seals:; = composting, in addition to adding appropriate micro-organisms to make the microbes fully active, maintaining the optimal growth conditions of the microorganisms , -Composting microbial strains = You can also strengthen the fermentation and decomposition of compost materials. Fertilization conditions, in wood chips, beans =, 7 to use natural composting method, that is, according to the moisture content of the heap and aeration, etc. ===; when the material size, in the compost material, and finally in the rot:... Microbial biotic strains can be used as microbes in the production of composting; == Fermentation and decomposition efficiency of composting materials, for different organic material properties, application, quotation 2 microbial _ 'will be an important step in the composting process _. The key mechanisms for the use of microbial strains should include the selection of appropriate micro-facilities &«(4)_$„_methods and m-methods for composting materials. Trichoderma (7Wd〇rma sp.) has been found in foreign studies. In addition to antagonizing some phytopathogenic microorganisms such as 猝 病 sp 及 及 及 及 及 及 及 立 枯 枯 枯 枯 枯 枯 枯 枯 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少 减少Absorption and promotion of 1287534 growth, increase yield, etc. Some scholars still use gene transfer technology to introduce disease resistance genes into plants to achieve disease resistance. Because of its many advantages, it has been studied abroad. The bacteria were developed into biological preparations for biological control. The commonly used ones were T.virens, T.harzianum, T.atroviride and T.asperellum. The crops used were mainly vegetables, fruit trees, flowers and turf. The strain of Trichoderma sp. (sp. TCT111) having the ability to decompose strong fibrous materials can be isolated and screened, and has the function of rapidly decomposing bulk organic waste containing rice husk, and can be used for fermenting of rice husk compost. The present invention is directed to a physicochemical property of rice husks, and a strain of Trichoderma sp. (7>ie sp) having a high ability to decompose fibrous materials is selected, which can rapidly occupy the materials in the early stage of composting reaction so that the materials containing rice husks are During the composting process, the fermentation and decomposing can be fully and quickly decomposed. The purpose of the soot is to provide a mold strain capable of decomposing the organic compost material containing rice husk: the strain is characterized by: 2〇. % quick red green when extracted twice; aerial hyphae are white; branching is two = conidial stalk width 4~4.5 a slender and slightly savage; sub #, branch and bottle stem mostly to S, part Three rounds S; the top bottle is 8~13~J round; the bottle stem is curved in the shape of an ampoule to a nearly conical shape, and the size is #面Γ/^33μηι; the conidia are spherical or short-shaped, and the surface is smooth. Part 3^4, TO8.3,, m; 12〇c,6 Another object of the present invention is to provide a strain of Trichoderma spp., which is deposited in the Republic of China food industry, which was established in the Republic of China in 1993. Center for the species, which is registered as 1287534, a Trichoderma strain numbered BCRC 9_71, or derived from the wood The Trichoderma strain of the present invention has high decomposition ability to the rice husk composting material formula, and can accelerate compost maturity, reduce composting time course, and reduce composting production cost. [Embodiment] The Trichoderma strain of the present invention can be separated. From natural sources, for example: from soil mites, crop roots and various rice husk composting products, microbial separation is carried out by 2% water agar (WA a) tea plate method, and 1 gram of soil is weighed by soil separator. After shaking in milliliters of sterile water, the filtrate was taken for scribing and separation. The roots of the crops were separated and the roots were separated. The roots were washed and cut into 5 min sections and then placed in WA·. The composting materials are first subdivided into 〇·5 cm small sections and then placed in WA· in 5 small sections. Each of the above-mentioned materials is 5 culture dishes, and each treatment is carried out in 28 incubators. After three days of illuminating, the tip of the hyphae is picked. On the potato glucose medium, after 7 days of culture, the growth morphology of each mycelium was observed, and the strain of the mycelium mycelium was picked from the strain, and the second pure line was separated, and the mycelium of 〇·5 cm2 was cut and placed. Spiral tube 5 cc of sterile water for storage. After the isolated strains were cultured for 7 days in pDA, the mycelial masses were excised, placed in a test tube containing 10 g of rice medium, and the mycelial entanglement and growth of each strain were observed, and the sporulation was observed to determine the morphological traits. , picking strains that can quickly entangle rice culture and sporulation. The strains selected for rice husk composting were identified by Trichoderma asperellum ( TCT111 ) Samules, Lieckfelds & Nirenberg, and the colony morphology was: at 2 ° C in MA2 (containing 2% malt extract) The medium grows rapidly (refer to the first and second figures), the cultured hyphae are white and sparse, and the conidiation is pustulate (refer to the third figure), and the sporulation is slow (5 days just after Started to form). In the microscopic structure: conidia 1287534 stalk (conidiophore) width 4~4.5//m, slender and slightly curved, branches are repeatedly branched (refer to the fourth, fifth and sixth figures) ), the branches and the phialide are mostly opposite, some three rounds, the top bottle stems 2 to 5 rounds; the stems are curved, the ampulliform is subducted, Size 8~13x 3~3.3 am; conidia (conidia) nearly spherical or short elliptical, mostly smooth surface, a small part of the surface is not obvious braided, size 3.2~4·0χ 2.8~3.5//m ( Refer to Figure 7 and Figure 8). The Trichoderma strain of the present invention may be a variant derived from a Trichoderma strain isolated from a natural source, and for example, these strains may be variants obtained by UV irradiation, ethyl methanesulfonate treatment, nitrous acid treatment or the like. It may also be a recombinant strain produced by genetic engineering using cell fusion or recombinant DNA techniques. The following examples are intended to further understand the advantages of the present invention and are not intended to limit the scope of the invention. Example 1 · Mud-flavor strain (7>/c/r unintentional sp·, TCT111) growth condition test and strain characteristics Trichoderma sp. (TCT111) original strain growth temperature is 12~36 °C, sporulation The temperature is between 24 and 28 ° C, the growth pH is between 5 and 8, and the optimum value is between 6.5 and 7.5. The strain is cultured in the squash culture medium of the horse yam, and after seven days of cultivation, it is made of sterile water. The suspension of the stalks lx 1〇3 spore/ml was added to the rice medium for two to three weeks, and the rice medium was autoclaved at 120 ° C with water in an un-shelled rice grain at a ratio of 3:1 by volume. Two times in 40 minutes, after the mycelium of Trichoderma is overgrown with rice grains, it forms green spores, which is the inoculum of mass production. When the inoculum is mature, the rice medium is taken out and disinfected, and the seed culture is harvested by sandwich culture. The strain and the sandwich sandwich culture method are cultured by the inoculum + disinfected rice + inoculum. After the inoculation source is fermented by this method, the rice medium is fermented into a solid strain and the spore concentration can reach lx 1〇12 spor*e/ g, production time can be completed within twenty days, than It fast strain more than 10 days. The solid strain prepared by the rice medium can be stored for more than one year at room temperature, and can be stored for a longer period of time at a low temperature (-4 ° C or -30 ° C). Example 2. Temperature effect of composting using Trichoderma The ratio of dry weight of each component of rice husk composting material was 55-65% of rice husk, 15-25% of rice bran and 15-25% of oil mash. Mix the compost material evenly and then use the appropriate amount of Trichoderma TCT111. The number of bacteria is about lx 1012 spore/ml. Diluted with water to dilute 200 times to form a bacterial suspension. The bacteria dilution is mixed into the compost material, and finally the moisture content of the compost material is adjusted. Up to 60%, the stacking height is maintained at about 1.5 to 2.0 m, and immediately after the accumulation, the stacking process is performed at about 5 to 7 Torr during the composting period, and the pile is doubled by the shovel loader until the compost is ripe. Table 1 below shows the temperature effect of the composting process, and CK is the control group that is not inoculated with Trichoderma. In the rice husk composted with Trichoderma, the accumulation of the second to third 曰, the temperature of the compost body rises above 60 °C. Up to 70 ° C, this high temperature period (> 60 ° C) is maintained for about 10 to 13 曰, then the temperature gradually decreases, about 31 to 35 曰, the composting temperature can be reduced to about 46 ° C, about 36 ~45 days, the compost temperature can be reduced to about 42 °C. The rice husk compost that is not inoculated with Trichoderma is deposited in composting for 4~6曰, the temperature of the composting body rises above 60°C, and the highest can reach 67°C. About 31~35 days, the composting temperature can be reduced to about 50%. Around °C, about 36~45, the compost temperature dropped to about 41 °C. It can be shown that the rice husk compost treated with Trichoderma can reach the maturity stage 5 to 7 days earlier. Table 1, the effect of inoculating Trichoderma (TCT111) on rice husk composting temperature 1287534 曰 number processing 0~5 6~10 11 〜15 16 〜20 21 〜25 26 〜30 31 〜35 36 〜45 Inoculation TCT111 62 70 66 57 54 50 46 42 CK 60 67 65 55 56 52 52 44 Units (°c ) Example 3: Effect of using Trichoderma on nutrient content of rice husk compost Composting was carried out as described in Example 2. The nutrient of composted rice husk compost The results of the content analysis showed that the nitrogen content of the bio-rice compost inoculated with (TCT111) was about 1.63%, the content of the structure was about 0.44%, and the unloading content was about 1.16°/. The calcium content is about 1.89%, the town content is about 0.88%, the organic matter content is about 67. 4%, the zinc content is about 55 ppm, and the copper content is about 17 ppm. The uninoculated rice husk compost has a nitrogen content of about 1.56%, a lining content of about 0.38%, a potassium content of about 1.13%, a calcium content of about 1.92%, a magnesium content of about 0.87%, an organic matter content of about 68.1%, a zinc content of about 49 ppm, and a copper content. About 16 ppm. Obviously, the content of nitrogen, phosphorus, potassium, zinc and copper in the rice husk composted with Trichoderma sp. was slightly higher than that of the untreated bacteria. The strains of Trichoderma inoculated (TCT111) can be isolated from the compost preparation process and the finished products such as rice husk compost. The isolation rate is about lx 1〇4 to 1 X 105spore/g. The added Trichoderma species can survive in the composting process and in the finished compost. Table 2: Effect of Trichoderma inoculation (TCT111) on nutrient content of rice husk compost Nutrients Nitrogen, Phosphorus, Potassium and Calcium Town Organic Matter Zinc Copper Treatment (%) mg/kg Inoculation TCT111 1.63 0.44 1.16 1.89 0.88 67.4 55 17 CK 1.56 0.38 1.13 1.92 0.87 68.1 49 16 In summary, the Trichoderma strains screened by the present invention have a high decomposition ability for the rice husk composting material formula 1287534, and can accelerate compost maturity, reduce the composting time course, and reduce the composting production cost, and have considerable economic benefits. Other embodiments of the present invention have been described in detail in the foregoing embodiments, and those skilled in the art can change and modify the present invention as needed without departing from the spirit and scope of the invention. Therefore, other embodiments are also included in the scope of the patent application of the present invention.
12 1287534 【圖式簡单說明】 第一圖係為本發明之木黴菌株TCT111在20°c時於含2%麥 芽萃取物之培養基中生長第五天的菌落形態圖。 第二圖係為本發明之木黴菌株TCT111在20°C時於含2%麥 芽萃取物之培養基中生長第七天的菌落形態圖。 第三圖係為本發明之木黴菌株TCT111之產孢區域外觀形態 圖。（在63X放大倍率下呈現苞狀突起） 第四圖係為本發明之木黴菌株TCT111之分生孢子柄及分枝 在200X放大倍率下之外觀形態圖。 第五圖係為本發明之木黴菌株TCT111之分生孢子柄及分枝 在200X放大倍率下之外觀形態圖。 第六圖係為本發明之木黴菌株TCT111之分生孢子柄及分枝 在1000X放大倍率下之外觀形態圖。 第七圖係為本發明之木黴菌株TCT111之分生孢子在1000X 放大倍率下之外觀形態圖。 第八圖係為本發明之木黴菌株TCT111之分生孢子在2000X 放大倍率下之外觀形態圖。12 1287534 BRIEF DESCRIPTION OF THE DRAWINGS The first figure is a colony morphology diagram of the Trichoderma strain TCT111 of the present invention grown on a fifth day in a medium containing 2% malt extract at 20 °C. The second figure is a colony morphology map of the Trichoderma strain TCT111 of the present invention grown on a seventh day in a medium containing 2% malt extract at 20 °C. The third figure is an appearance pattern of the sporulation region of the Trichoderma strain TCT111 of the present invention. (The ridges are present at 63X magnification.) The fourth figure is the appearance of the conidial stalks and branches of the Trichoderma strain TCT111 of the present invention at 200X magnification. The fifth figure is the appearance pattern of the conidial stalk and branches of the Trichoderma strain TCT111 of the present invention at 200X magnification. Fig. 6 is a view showing the appearance of the conidial stalk and branches of the Trichoderma strain TCT111 of the present invention at 1000X magnification. The seventh figure is an appearance pattern of the conidia of the Trichoderma strain TCT111 of the present invention at 1000X magnification. The eighth figure is an appearance pattern of the conidia of the Trichoderma strain TCT111 of the present invention at 2000X magnification.