TWI277420B - Medicine using anemonin as active pharmaceutical ingredient for treating aseptic inflammation - Google Patents

Abstract

This invention reveals a medicine for treating aseptic inflammation. Wherein, the active pharmaceutical ingredient contains anemonin. Especially, it reveals the application of anemonin compound in treating aseptic inflammation. The active pharmaceutical ingredient in the medicine is extracted or separated from natural plant containing anemonin or the precursor thereof. It also provides the method for preparing the anemonin extraction solution. The medicine is prepared as oral medicament, injection, and external use. Particularly, it includes various preparations for external use capable of being absorbed through skin, like fluidextract, plaster, suppository, liniment, and paste etc.

Description

1277420 V. INSTRUCTION DESCRIPTION (1) FIELD OF THE INVENTION The present invention relates to inflammatory drugs, n; the field of medicine, in particular, -#, Λgkd*, especially white s:: and preparation methods of the drug; The application of sin in the treatment of aseptic inflammation is effective in the background of the invention. Two major categories, bacterial, two, bacterial inflammation J and "sterile # pay attention to, a large number of f can easily lead to the onset of acute illness, 21 Antibiotics as a right f bacterial infection drug such as Penicillium:: introduction bed, and aseptic inflammation in the = ^ was developed and used in the effective treatment of drugs, it is different 4 special effects or even In the international medical community, it is completely from the treatment of aseptic inflammation of si K to produce anti-sterile inflammation::; no effect = He-home pharmaceutical - including: cervical spondylosis, lumbar spondylosis 'periarthritis, tennis: n; Syndrome, rheumatoid arthritis, osteoarthritis, muscle cold bone cold:::= adenitis, polyneuritis, neurodermatitis, tendon sheath [waist two; bone necrosis, bronchial asthma partial: m y sense A disease of fecal inflammation. Modern medical research & Second, 疋 /, 匕 belongs to the surrounding soft tissue, inflammatory exudation, edema, and metabolite mrrr Shenjing sensory secret, from the vertebral vertebrate ^ \ 卜 injury itself and secondary a series of pathological changes caused by aseptic inflammation in the muscles, fascia 1 band, peripheral plexus,

Page 4 1277420 Five inventions description (2) The edema and adhesion of the sac and blood vessel wall compresses the ridge - ^^ root, which makes the complex symptoms of cervical and lumbar spondylosis difficult to eliminate, vertebral artery and nerve belt, tendons and muscle traction Many aseptic inflammations and 24 weeks of periarthritis, tennis elbow, myofascial syndrome, bone = °. cervical and lumbar spondylosis, the cause of shoulder, these aseptic inflammation caused by the physical fitness of the scale But the diseases that are still plaguing billions of people around the world are mostly chronic diseases. The invention outlines the various diseases and stubborns that are currently available for the treatment of aseptic effective inflammatory pain. For the treatment of aseptic inflammation, Jf Ming is a music that contains a compound whitehead, and its active ingredient is included. In particular, the compound can be obtained by chemical synthesis to obtain an effective activity into an eight system: Original. In the clinical treatment, it is proved that the common, multi-ample, and medicinal drugs for the pathology have many ailments of the inflammatory inflammatory disease, and the intractable pain of the physiology is difficult to cure and the body is removed by aseptic inflammation. The curative effect can eliminate edema and adhesion caused by aseptic inflammation and adhesion, as well as aseptic inflammation and water treatment to cure various aseptic and painful body disorders, so as to be efficient, rapid and safe. The white inflammation disease is non-toxic and non-irritating. The use of the present invention for the treatment of a non-toxic compound or a natural extracting compound, Pulsatilla chinensis, containing pulsatilla. The present invention also proposes a pharmaceutical form for treating aseptic inflammation in this dosage form, especially the external: active ingredient. Agent' provides optimal administration for the treatment of aseptic inflammation

1^77420 ·

The present invention also provides that the active ingredient in the formula is a natural medicine derived from aseptic inflammation, and the present invention simultaneously extracts the extract of the pulverizer. A method for preparing a natural medicinal plant for extracting a drug, in particular, a method for providing a raw material=a preparation of a pulverizer from the method provided, and converting into a thorn thorn by the present invention: w: containing a strong toxic side effect The original whitening production. DETAILED DESCRIPTION OF THE INVENTION The invention provides a therapeutically effective amount of a compound configuration: The medicament for treating aseptic inflammation includes pulsatilla, the compound having The following chemically and illegal compounds may be derived from any extract of natural Chinese herbal medicine containing edulis and protothecin. Suitable natural Chinese herbal medicine materials may include ranunculaceae and gramineous plants. Among them, 'Mulberry plants (conditions (3) this buckle-like plant) include • Ranunculus japonicus Thunb., Caltha palustris L., Ramin cuius ^ceieratus L., broken bowl flowers ( Hupehensis Lem·), Pulsatilla (Pzx/plus chinensis (Bge.) Regel), bamboo scent

5163-4131-Pf.ptd Page 6 1277420 V. INSTRUCTIONS (4) (Anemone raddeana Regel Ranunculus

Chinensis Regel ), Anemone altaica Fisch, etc., and chinensis osbeck containing pulsatilla, Unetiana Ievi et, and clematis: hexapetala H), Clematis Manshurica Rupr·, paniculata, Clematis cktsiandra Μΰχ/m, Clematis uncinata champ.e:c Benth), Clematis meyeniana Walp, and other nine species, and a variety of autumn peony that breaks the flower of the bowl (Anemone hupehensis Lem. var. japonic (Thunb.)

Bowles et Steam), etc.; root of Imperata cylindrical in gramineous plants, Ranunculaceae Helleborus plant containing hairy ridge (), anemone (乂) plant of the anemone family Phytophthora (valence <3) plants, Ranunculus, Bratrachium, and the genus Aconitum scaposum var · vaginatum). In the previous public report of the present invention, the above-mentioned natural raw materials contained trichostatin or protothecin, which has been confirmed by clinical experiments to have a certain anti-cancer activity, and has an inhibitory effect on tumors, especially the treatment of fresh edulis externally, and the history is very It has a long-term use and is very effective. It has a good effect on many common diseases such as frozen shoulder, rheumatoid arthritis and sciatica. However, the original pulsatilla has strong irritancy and toxicity, such as making these plants contact with the skin for a long time.

5163-4131-Pf.ptd Page 7 1277420 V. Description of invention (5) — Causes inflammation, such as redness and irrigating 笙 茛 解 解 解 成为 成为 成为 成为 成为 & 为 为 为 为 为 为 为 为 为 为 为 为 为 为 为 为 为 为 为Polymeric Pulsatilla, part of the original: Rui: f white sinensis can be polymerized (mostly the raw material medicine produced during the process of placing, also contains Staphylococcus aureus, Streptococcus, diphtheria, :? η 2 For the clinical treatment of tumors and anti-bacterial anti-inflammatory; (2) for the anti-celery in the clinical use of rheumatism, activating blood and relieving pain in the long-term research of the inventors of the insults such as Welling; some of the original contains the original Pulsatilla Including the above-mentioned raw materials in use, all have a stimulating effect on the human body. No matter whether it is internal or external and poisoning symptoms, it causes noise: internal medicine can cause severe eosinophilic hematuria, proteinuria; diarrhea with the skin and even stimulate the kidney to produce this ingredient There is a strong anti-inflammation that triggers inflammation such as redness and blisters, but the solvent, and long-term placement (or heating m department = adding water or organic sulfuric acid to make the original pulsatilla two molecules Nie, or adding ether, acetone, for no Bacterial inflammation is intractable, and the above-mentioned side effects disappear, but it has the special effect of rapid healing, especially: the treatment of bic dysfunction Φ. The effect of dry transdermal administration is extremely effective and the chemical structure of the pain zone is used. In this process, 疋: μ疋 raw material in the original white stalks even if the original pulverizers have changed in two molecules, the prime. The results of this study are based on the irritating Pulsatilla main active ingredient for the treatment & a drug for non-functional inflammation, in which

5163-4131-Pf.ptd

Page 8

1277420 V. Description of invention (6) Pulsatilla can extract products, gp is one of the external rheumatoid joints according to medical inflammation including ·· § complication, rheumatic adenitis, multiple due to aseptic inflammation headache, stubborn bone extract In the case where the conventional analgesic drug of the present invention is also temporary, the drug of the present invention can be used for various drugs, without injection, introduction, no hospitalization, and wet application for three times to pass the pain in the affected part. Absorptive capacity, key, fascia, dysentery, rapid elimination of treatment for massage, treatment or use of extreme moxibustion, no Pulsatilla treatment mechanism for Pulsatilla, and annual deposition of wall bundles and = chemical synthesis products, or from = The classification of various types of inflammation of the extract of Pulsatillae, disease, lumbar spondylosis, scapulohumeral inflammation, osteoarthritis, myocarditis, neurodermatitis, sciatica caused by disease, 2, cancer pain, bronchial fever Three times, some only treated ^ 丨 性 osteitis, migraine 疋 have painless inflammation. In the past 'treatment of these diseases' or physical therapy such as massage, the 'serious person needs to provide the treatment of aseptic inflammatory diseases of the pulsatilla extract by hand. No infusion, no surgery, no needle only by external application, such as healing; The treatment of the drug of the present invention is targeted to transdermal administration, and the use of swelling, dispelling, and decomposing adhesions to remove multiple segments, bursae, nerve plexus, blood edema, and adhesion removal, loose natural drug substance in a very short time. The separation is provided. The sterility, tennis elbow, myofascial collateral cold, aseptic front tenosynovitis, lumbar muscle strain according to the present invention; foot root pain, migraine, partial asthma, rapid recovery using pulsatilla once, syndrome, technical air tube The cause of asthma is to take traction at the time of onset. Compared with the prior art, Pulsatilla is made, not to be massaged, and the extract is not transdermal. It is a very strong town of Pulsatilla. It is very strong in the muscle, the aseptic inflammation between the muscles has been #5化的刃

1277420 V. INSTRUCTIONS (7) Belt, muscle relaxation, adhesion of blood vessels to increase, and blood pressure, periorbital inflammation, humeral pain, migraine, strain, osteoarthritis, tenosynovitis, pain caused by the syndrome Light-stressed vaginal adhesions and dyskinesias are also based on the use of Pulsatilla, with or without the use of pharmaceutical methods, in this topic, topical agents, liniments, coatings using synthetic or natural drugs such as scientific publication pages The prescriptions, oral preparations, and pulsatilla to a large amount of blood expansion exudate to reduce the sterility and eliminate phlegm, muscle tendons, stomach pain, inflammation, aseptic multiple nerves, and the hands of 'elimination of stimulation The exercise may also include the selected type of the medicine provided by the aseptic inflammation. The invention may be made into a variety of materials, such as a translucent agent, which can be used as a raw material. The liquid is made into a metabolite that enters the muscles and causes the treasure to have fy σ, such as the shape of the sputum: after the circulation is improved, the muscle tension is stimulated and the sleeves of the muscles are compressed. The more cervical spondylosis, the knowledge of the sputum, the spleen spleen, the shoulder W syndrome, the sciatic arthritis, the muscle cold bone cold feet; muscle = inflammation, old bruises, pain, pain, treatment, fg _ wide-ranging disease The principle of sacral spondylosis, lumbar spondylosis, and muscle is completely the same. The swelling and pain caused by strong pulling of the muscles of the sacral sputum Μ 恢复 消除 恢复 恢复 恢复 恢复 恢复 恢复 , , , , , , 肿 肿 肿 肿 肿 肿 肿 肿 肿 肿 肿 肿 肿 肿 肿 肿The auxiliary agent, the auxiliary agent, the final dosage form of the drug, according to the conventional oral preparation, the injection and the external preparation, the skin absorption immersion agent and the plaster suppository dosage form. The preparation in the medicine can be directly extracted and separated. The pulverizer can also be extracted by an open method, Chinese herbal medicine chemistry" (1 9 77), 226 pure pulsatilla crystals are produced by injection from natural plants containing edgy ridges (the main ingredient is Pulsatilla)

1277420 V. INSTRUCTIONS (8) To play a role in the treatment of various diseases such as infinite inflammation. :: Steady, w < manifested in: Pulsatilla extract in a variety of strict production, feather stability, dish, mildew, rot and smell, its beneficial bacteria such as high temperature, low cure Will not decay. According to a preferred embodiment of the present invention, the household: the straight or the grass is the original material, and the "agent: amphoteric: disease oil,: vinegar or inorganic acid is used for leaching" The agent is leached to obtain the water, the vinegar or the inorganic acid to act on the water, the movement, any natural plant or Chinese herbal medicine in the sealing strip; = the master or its liquid is filtered, clarified and other refining processes to obtain the pulverizer Use the original: Phyllostachys pubescens including: ^ Breaking the bowl of flowers, Pulsatilla, bamboo scent, nine: ^, and from the clematis containing the white-headed sage: Clematis: n哉ϊ莲毛九:花花莲,毛蕊铁线^柱= ί禾本和破:花花的种秋秋牡丹; 物,哿莲 m分U毛毛茛的毛茛科铁子子属: Plants , choose the ear genus genus ... family, etc., you can also use traditional Chinese medicine decoction pieces, such as Baitou Cui::: middle head

11

I 5163-4131-Pf.ptd Page 11 1277420 V. INSTRUCTIONS (9) The organic solvent used includes ethanol, methanol, propylene glycol, and the like. The immersion of the cold-immersed glycerin, chloroform or the extracting agent under the temperature of the present invention means that the raw material drug is subjected to the usual hot dip method, i.e., the raw material drug can be used. The method of the preparation method provided by the present invention is carried out by the method of the preparation method provided by the present invention. One of the raw white materials in the raw material medicine is that it is converted into a pulverizer by polymerization: a structural change 'sterile,' Significant curative effect of ΐ ΐ 炎 仙 , , , , , , , , , , , , , , , , , , , , , , , , , , , iii ii ii ii ii ii ii ii ii ii ii ii ii ii ii ii ii ii ii ii ii ii ii ii ii ii After heating it to the boiling point, it is added to the original 枓乐继彬 to heat to the boiling point, and the sealing leaching is carried out for more than 6 months. According to the preparation method of the invention, the supporting part, such as fresh hair sound = straight, the effective selection of the raw material medicine can be selected. The proportion is added to the dip sword; after the root is crushed, it is put into the extraction tank, and the ϊΐ: ίί is converted into a pulsatilla dissolved in a solvent, and then expanded by 1 = male filtration and clarification, and a yellow transparent clarification method is obtained for detection). The white-headed feather can be passed through the conventional formula, such as tΠ'. The special feature of the preferred embodiment of the present invention lies in the fact that the original protothecin is converted into a disintegrating substance under the condition of 'the original #药与适(四)量The extracting agent together: sealing for a long time Process. Leaching agent selected as listed above, may Cloth hot methanol or methanol with cold methanol 30% degree Creek, vegetable oil, for example, may be hemp

1277420 V. DESCRIPTION OF THE INVENTION (10) Oil, tea oil, peanut oil, etc. 'Ethanol, methanol or water is preferably used, and the drug substance can be extracted by one or more kinds of mixing. For example: according to the weight ratio, choose fresh 茛: ethanol ratio of about 1:1, take fresh hair, whole grass, root or only whole grass, crushed and put into the container, the moderate concentration, for example, about 4% - After heating with ethanol, pour it into the volume P containing fresh bristles, continue to heat, seal and store, so that the original pulsatilla in the edulis is fully polymerized into pulsatilla dissolved in the solvent, then pressed, slag, filtered, clarified, taken The yellow transparent clear liquid is the extract of Pulsatillae; the fresh buttercup can also be selected according to the weight ratio: the ratio of ethanol is about 1 ··1, the whole grass is taken from the whole grass, the root or only the whole grass is used, and the container is crushed and put into the container. Inside, use a cold soak method to directly pour a moderate concentration of, for example, 40% ethanol or a concentration of about 30% of methanol into the container of the freshly colored donkey.

Long-term release, so that the original pulsatilla in the edulis is fully polymerized into a pulsatilla dissolved in a solvent, then pressed, decomposed, transitioned, clarified, and the yellow transparent clarified liquid is obtained as a extract of Pulsatilla; or can be extracted using traditional Chinese medicine pieces. For example, if the traditional Chinese medicine Weilingxian decoction pieces, Pulsatilla decoction pieces, and the extract of Pulsatillae by ethanol hot dip or cold soaking method, the ratio of ethanol: the ratio of medicinal materials is about 3:1 'with a moderate concentration such as 40% Ethanol hot dip method or cold soak method for three to three months, once a day to mix, so that the Chinese medicine Wolverine or Pulsatilla can be fully dissolved in ethanol, filtered to clear the brown pulsatilla extract; If water is used as the solvent, the fresh ranunculus powder is finely calculated according to the weight ratio: water is about 1:1, and an appropriate amount of preservative (food grade) is added. The water is poured directly into the package by the cold soaking method or hot dip method. In a thin container, sealed and stored\The original Pulsatilla in the hairy hair is fully polymerized into Pulsatilla in the solvent, then pressed, slag, filtered, clarified, and the yellow transparent clear liquid is obtained.

5163-4131-Pf.ptd Page 13! 277420 Ming through the raw materials in the Pulsatilla «ΧΙΟ ArAr 1 * tune, so the grid is limited to the sterility of the Chinese herbal medicine compatibility, this case, the workers can choose to use from the above super The critical separation of the aseptic auxin is normalized according to the five, invention description (Η) Pulsatilla extract. It should be noted that the operation of the present invention is aimed at extracting the ointment and extracting the original extract of Pulsatilla, which is used in the preparation of the desired dosage form, and does not need to be strictly in accordance with the present invention in the preparation process. It is preferred that the stability of the extract of the solid and one or more of the hormones is not independently and stably utilized independently of the various effects. Long-term placement tests have shown that the sex is more than 5 years. According to the embodiment of the present invention, the method of production is selected, that is, the lysin compound is used as a raw material, and the extract is also extracted, and the extraction method is divided by the forced circulation process of the extraction tank, or the active ingredient fraction of the Chinese herbal medicine. The present invention also provides an adjuvant, if necessary, for the treatment of a therapeutically effective amount of the above-mentioned ▲ head, an agent, an injection, and an external preparation. Use a suitable leaching agent to completely polymerize the original pulsatilla and white pigment into a pulsatilla, and the concentration is required to treat the leaching agent and the drug substance. The extracted pulverizer or whitehead or chemical When mixing, the Chinese medicine of Pulsatilla chinensis and its chemical components are treated in various diseases. In the stable production of Pulsatilla, the Pulsatilla extract can be selected according to the actual conditions. Extraction by extraction, extraction techniques, or dynamic extraction techniques, or the use of a pharmaceutical composition for distilling inflammation, a clathrate thereof, and a pharmaceutically acceptable pharmaceutical method to prepare an oral external dosage form including transdermal absorption

1277420 V. Invention description (12) type of two π «agent, hard high agent, test agent, smear, coating agent and other agents get two: head 2:: 3 0 - 40% concentration of ethanol according to this The invention method is applied after the adsorption of the cloth 2: liquid: as a flow agent, can be used for rubbing the affected part or by the yarn, fresh leather is poor /... Right with ethanol as a preservative, the ethanol by weight ratio is directly fine: ethanol is about 3:1 The ratio will be 95% long when the id is filled into the container with a small hairy smiley powder, and the seal is smashed into heat: the child puts the original white-headed sage in the fresh hair and laughs. That is to pass the white-headed ointment ointment. It is extracted by the same method as the Uif ‘ 威 饮 、 , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , Ϊ二:1 every 2= or outside for the treatment of aseptic inflammatory disease liquid, about 1, pulsatilla = white f yin liquid or white-headed scented 25 ml white head now to Mnan, pike, only need to use every week Gauze absorbs about the pain area: Apply a J in: bacterial inflammation, various disease lesions or hours, three times - treatment? = product is about 100 (four) 2' each time there is an analgesic effect in four rooms = ί is as high as 9〇%' short-term application can be cured, no redness, efficacy, some patients - secondary use, safety range, toxicity m water , allergies, pain and other by-products to make a flow extract, plaster, plug: = cephalosporin liquid or extract can be formulated, pulsatilla can also be made: ^ agent, coating agent and other external use pills, oral liquid, Medicinal wine cellar. Tablets, injections, and the like, may also be formulated into a drug or a drug group provided by the present invention. The compound is compatible with adjuvant treatment = the intermediate withdrawal may include any sputum and ‘, 糸 & The traditional Chinese medicine can

!277420 V. Description of invention (13) 5 From spatholobus, big blood vine, mountain branch, roadside gold, etc. It should be understood that the present invention has the use of Pulsatilla and the use of Pulsatilla chinensis, and at the same time, by the polymerization of the effective cephalosporin, the stimuli are improved from the original pulsatilla to the whitehead, especially to the skin. The treatment of aseptic inflammation is cervical spondylosis, lumbar spondylosis, shoulder pain, old bruises, aseptic solid stomach pain, cholecystitis, etc. The invention is based on the treatment of aseptic inflammation by various kinds of aseptic inflammation containing Pulsatilla. (In pain 1, there is indeed an acute toxicity and long-term toxicity test using the present invention as an active agent, and the most priming poisoning or death of guinea pigs during intraperitoneal administration; dermal administration to animals fails to cause motion 4 Zhou's failure to cause guinea pigs, body weight, kidney function, internal organs and skin animal test results can also be obtained from tea, Yanwujia, Yemenmengen, iron chopsticks, the main feature is the private > Ingredients in the treatment of aseptic inflammation promote the original structure contained in the raw materials, become the ginseng, due to the transfer of Mp;, eliminates the original Pulsatilla sex, through the external number #, bottom ^ pr / D treatment of the best dose of various diseases, such as Western medicine, rheumatoid arthritis, sciatic prostatitis, bronchial asthma, The stubborn "itchy" has obvious curative effect, that is, the research and application of the drug has achieved the right, and the bed test proves that the effect of the medicine of the invention is remarkable, and the effect of the muscles of the muscles is relieved, the swelling and the pain are relieved. The results were obtained from the extract of Pulsatilla chinensis. The large tolerated dose was 266 g/kg, and 10% of the surface area of the unexposed body was used as the poisoning or death of the administered area. The continuous skin gave the visceral coefficient, blood picture, liver function, Abnormal and pathological changes. The drug of the present invention is non-irritating,

5163-4131-Pf.Ptd Page 16 1277420 V. Description of invention (14) Non-sensitizing substances, non-irritating to the skin, no allergic reaction, no damage to the skin pathology, so it does not hinder; skin wound = make The specific embodiments and functions of the present invention are described by way of example, but the implementation of the present invention should be based on the scope of the claims, and any solution based on the idea of the present invention, that is, using various means, It is within the scope of the present invention to change the chemical structure of the protochrome, and to polymerize the sputum into white: different forms of the drug containing pulsatilla or related ingredients for the treatment of sputum, ..., sputum inflammation. Example of ear application Example 1 Take fresh buttercup whole grass and root or only whole grass from April to September, put 100 kg of fine powder into a ceramic or plastic bucket, heat 40% ethanol to boiling point, then pour It is sealed in a container with fresh bristles for more than half a year, so that the original pulsatilla can be fully polymerized: whitehead in solvent, pressed juice, decomposed, transitioned, clarified, and taken yellow transparent clarified liquid to obtain Pulsatilla extract, routine method test, The active ingredient of the extract contains about 20% of the pulverizer content. It is formulated with ethanol to contain 12. 5 mg of pulverulent per 25 liters of extract, which is made into an external preparation for external treatment. Or use ethanol cold dipping method k for more than one year after the long-term extraction of Pulsatilla extract 0

Face 5163-4131-Pf.ptd Page 17 1277420

Example 2 Flower, 2: Plant: + Γ fresh 茛, horseshoe leaves, stone dragon 芮, break the bowl of flowers, nine sections of Fu Pu, break the bowl of flowers, fragrant, return to garlic, use only single - middle grass, mining: history The whole grass and roots of the mixed autumn peony or the extract of Pulsatillae; the glycol extracting method or the cold soaking long-time rubbing agent (the external coating agent can also be prepared by the method of extracting these plants V or the third embodiment) Chinese herbal medicine Weilingxian decoction tablets, or Clematis and white-head extracting method to obtain the extract of Pulsatillae, according to the ratio of ethanol: decoction pieces, 3: ', i, with 40% concentration of ethanol by heating and leaching method In the month, stir once a day to dissolve the pulsatilla in the Chinese medicine Clematis or Pulsatillae in ethanol, and clarify by filtration to obtain the brown pulverizer extract. It can also be concentrated and refined and granulated to form a concentrated granule of traditional Chinese medicine. Example 4 3 乙醇 kg of ethanol with a concentration of 40%, and cold immersion method with ethanol at room temperature to clarify the whitehead contained in the Chinese medicine Clematis, and then extract the pulverized pulverizer into a concentrated granule of traditional Chinese medicine. 'King, Chinese medicine Willing Xian Pie Tablets 100 immersed for six months, once every time , Weng Su is fully dissolved in ethanol, the filtrate can also be concentrated and refined and granulated. This example can also be used to mix Pulsatilla or Chinese herbal medicine Qilingxian and Baitou Weng Decoction. 'Phosphorus extraction with ethanol at room temperature and cold soaking method liquid.

5163-4131-Pf.ptd Page 18 1277420

Example 5: Using water as a solvent, fresh buttercup powder, finely pulverized with 0 kg, and a concentration of 9 5% of ethanol, 33 f kg, directly poured into a very fine container containing fresh glutinous rice powder, and thoroughly stirred, sealed, heated or at room temperature. Store 8 - 丨 for more than 2 months, so that the original white in the fresh edulis, feathers are fully polymerized into pulsatilla, and depending on the consistency of the extract, add Vaseline to make it evenly, that is, it becomes a white-headed ointment. Example 7

According to the "Chinese herbal medicine (1 997) published by Science Press, the method described in the first... The whole body of the sage is steam distilled, and the extract of the distillate is purified by ethanol and refined with ethanol to make a crystal of pulsatilla ( Or purchased), it-steps are made into various dosage forms such as external preparations, oral preparations, injections, suppositories, liniments, etc. Example 8 Using the extract of Pulsatilla obtained in Example 1, it is formulated into about 12.5 per 1 ml of Pulsatilla containing 25 ml. The milligram of the liquid is soaked in a non-woven fabric to make a wet tissue with an area of 100 cm2.

Example 9 In K, the mixture was mixed into each topical treatment time for every 2 5 treatments. The powder of white pulverizer was weighed and added to the fresh hair. The ointment containing 1205 mg of pulverizer was added for 5-6 hours.

5163-4131-Pf.ptd

1277420

V. INSTRUCTIONS (17) Pharmacodynamic test The external application agent of Example 1 was used (trade name test: 1. The formaldehyde method analgesic test Guanyin dew was performed on the following 18122 g mice 80, male and female, random, and Medium concentration group, low concentration group (according to 丨, , and. high concentration 75 ml / 25 ml, 6.25 mg / 25 ml to give ^ 克 ^ 5 ml, 8. ίΒ ί 9 R i / 1 gauge circuit ), Du Lengding, and grams of kg) control group (administer an equal amount of 25% ethanol). In addition to Du Leng

The animals in the group were administered intraperitoneally, and the other groups were smeared with drugs on the left and right hind legs. 15 minutes after the drug, each mouse was injected subcutaneously with 2.5% formaldehyde 〇·〇3 m 1 ' and the drug was applied once more, and then the number of times of sputum hind foot in the mouse within 5 minutes was observed. 0 Result: High concentration of Guanyinlu There was a significant analgesic effect on the pain caused by formic acid. The number of right hind legs in the mice was significantly reduced. Compared with the control group, ρ < 0 · , see Table 1.

5163-4131-Pf.ptd

Page 20 1277420 V. Description of invention (18) Table 1 Inhibition of Guanyin Dew on lameness reaction (^=ST>) Number of animals in the group 舔P. 錾P value rate control group 16 15.68 Soil 4.13 ～s ---------- High concentration group 16 9_13 Soil 6.20 < 0.05 42 Medium concentration group 16 12.44 ± 7,20 > 0.05 21 Low concentration group 16 13.79 ± 5.33 > 0.05 12 Du coldding group 16 0_00 ± 0.00 < 0.001 100 I. Hot plate method Analgesic test using 55 °C hot plate to screen out the pain response positive = gram 'randomly divided into 4 groups: Guanyin high concentration group ^ sound, heavy cold Ding, and, control group, The method of administration and the amount of the drug, the formaldehyde method, the H, the sputum and the left and right feet were applied to the drug, and the sputum was in the abdomen 55. . On the hot plate, measure separately, 30 60, 9 〇 "Li, put the small gas in the result: Guanyin Luyan μ, rat pain reaction stagnation time., mouse pain response latency ^ = degree on the hot plate pain The obvious analgesic effect, increased, compared with the control group ^, prolonged 'the percentage of pain threshold increase in mice is obviously 'ρ < 〇·05, the results are shown in Table 2 and Table 3 〇1ϋ^ 5163-4131-Pf.ptd 21 pages 1277420' V. Description of invention (19) Table 2 Effect of Guanyin dew on latency of pain response (ISO) Number of animals in the group of pain response latent latency (seconds) After drug premedication 3 points after drug dispensing 9 〇 对照组 control group 16 29.33 ± 4.12 30.13 ± 5.19 29.69 ± 7.33 29.21 ± 8.18 high volatility group 16 28.46 ± 3.49 31.33 ± 7.21 45.49 ± 9.47 47.34 ± 10.29 P > 0.05 < 0.05 < 0.05 low concentration group 16 30.12 ± 5.03 31.78±6.32 35.26±7.79 36.30±9.74 P >0.05 >0.05 >0.05 Du Lengding group 16 30.08±4.92 49.8615.23 54.29±9.27 58.31±11.23 P <0.01 <0.001 <0.001 Table 3 Guanyinlu Effect on the percentage increase of pain mites - Number of animals in the group - Percentage increase in pain threshold (%) 30 points after drug administration After 60 minutes of medicine, 90 points of the control group, 16 3 1 0, high concentration group, 16 10 60 66 ί, agronomic group, 16 6 17 21, dultion group, 16 66 80 94 3. Anti-rat foot swelling, 40 rats, 200 -250 g, both male and female, randomly divided into 4 groups: high concentration group, low concentration group (12.5 mg / 25 ml, 6. 25 mg / 2 5 m

5163-4131-Pf.ptd Page 22 1277420 V. Description of invention (20) liter), Du Lengding group (25 mg/kg), skin relaxing group, control group (administer an equal amount of 25% ethanol). The animals in the skin-releasing group were given 〇·025% skin relaxing ointment, and the other groups of animals were applied with drugs on the left and right hind legs. Apply 1% carrageenan 1 1 m 1 to the right hind foot of each rat and then apply the drug to the right hind foot. The circumference of each foot was measured at 2, 3, 4, and 5 hours after administration, respectively, and the difference in circumference between the two feet was used as the degree of swelling. The rats were given a drug to the right hind paw after each measurement. RESULTS: High concentration of Guanyin dew had a significant inhibitory effect on paw swelling in rats, compared with the control group 'P< 〇 · 〇 5. See Table 4. Table 4: Inhibition of foot swelling by the Guanyin dew (the number of animals in the appropriate group) - swelling degree (cm) 2 hours 3 hours 4 hours 5 hours control group 10 1.44 ± 0.21 1.48 ± 0.13 1.47 ± 0.19 1.45 ± 0.13 high concentration Group 10 1.24±0.23 1.01±0.17 0.97±0.21 0.92±0.18 p value&gt;0.05 &lt;0.05 &lt;0.05 &lt;0.05 Low Degree of Agriculture 10 1.37±0.19 1.36±0.21 1.3S±0.24 1.40+0.14 ρ value> ;0.05 &gt;0.05 &gt;0.05 &gt;0.05 Widely relaxing ·Group 10 0.91 ±〇.2〇0.92±0.13 0.90+0.18 0.92±0.24 ρ value &lt;0.01 &lt;0.01 &lt;0.01 &lt;0.01

4. Tests of guinea pig in vitro and intestinal muscles 4 guinea pigs, 300_350 g, 2 males and 2 females. Immediately after sacrifice, the ileum was taken out and placed in oxygenated cold Tyrode's solution. After rinsing, the intestines were cut into

5163-4131-Pf.ptd Page 23 1277420, V. Description of invention (21) 2-2. 5 cm intestine segment. The two intestine segments are threaded at both ends, one end is attached to the venting hook, placed in a constant temperature Mail's bath, and the other end is attached to a tension transducer to connect to the recorder. The contraction and relaxation of the intestinal muscles were recorded after the addition of Guanyin, acetylcholine (1 〇 _9) and atropine (0 · 0 5%). Results · The high concentration of Guanyin dew has a significant relaxing effect on the intestinal muscles under the excitatory state of acetylcholine.

5163-4131-Pf.ptd Page 24

Claims (1)

1 . An external preparation for treating aseptic inflammation, wherein the therapeutically active ingredient is pulsatilla' and the pulverizer liquid for preparing the preparation contains 1.25 mg of pulverizer per 25 ml of the solution. The external preparation for treating aseptic inflammation according to the above aspect of the invention, wherein the pulverizer liquid is derived from an extract of any natural plant or Chinese herbal medicine containing pulsatilla or protothecin or edulis. 3. The external preparation for treating aseptic inflammation according to claim 2, wherein the natural plant or Chinese herbal medicine comprises: Ranunculaceous plant T (Ranunculus japonicus Thunb.), horseshoe Leaf Q Ca〗 tha pa 』usiris L·), Rantinciilus sceieratus L·, Anemone hupehensis Lem., Pulsatilla chinensis (Bge. Regel), Anemone raddeana Regel ), returning to garlic (Rmwncuhis Chinensis Regd), Anemone a 1 taica Fisch, selected from Clematis chinensis osbeck, Clematis flnetiana levi et Van, cotton clematis (Clematix hexapetala) Pall), Clematis Manshurica Rupr., Clemtis paniculata, m H Ciematis fiorida, Gienmtis ci^siandrst Maxim, Clematis Uncinata champ, ex Benth ), Clematis meyeniana Ψαΐρ ) Clematis and a variety of autumn peony that breaks the flower of the bowl ( Anemone hupehen Sis Lem. var. japonic (Thanh ) 5163-4131-PF2.ptc Page 25 1277420 Μ% 90115998 Bowles et Steam ); gramineous plant Rhizoma chinensis (r 〇〇t〇f hiperata cylindrical), Phyllostachys pubescens (Rammctdaceae SeUeboms) plant; and Anemone family of anemone ()獐 细 属 ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( 4. The external clothing pair for treating aseptic inflammation as described in claim 2, wherein the whitehead feather liquid is made of decyl alcohol, ethanol or water &gt; and k; The external preparation for treating aseptic inflammation according to the invention of claim 1, wherein the pulverizer liquid is ethanol of pulsatilla The external preparation for treating aseptic inflammation according to the invention of claim 4, wherein the pulverizer liquid is derived from an extract of ethanol to natural herbal Chinese herbal medicine. A system 7] as claimed in claim 6 The treatment described in the item Exogenous preparation of sexual inflammation, wherein the pulverulent liquid is from 30 to 50% of ethanol for immersion and is heated to the boiling point, and then added to the natural plant or Chinese herbal medicine to continue heating to the boiling point, and the sputum is sealed for more than 6 months. And the external preparation for treating aseptic inflammation as described in claim 6 wherein the white pulverizer liquid is from 30 to 50% ethanol as a leaching agent. An extract prepared by cold soaking for more than 8 months. 9 · Aseptic treatment of aseptic inflammation as described in Shen Qing Patent Range Nos. 1-8 12?7420 _η --------_ Case No. 901159^ 曰 Amendment VI, the scope of application for patents, hard ointment, ointment,:: preparation 'is a perfusal excrement, towel, suppository , liniments and coating agents. 4 1 0 . As described in claim 9 of the scope of patent application, it is a wet tissue which is made of a non-woven fabric soaking liquid and g=inflamed outside the area of 1 cm 2 : a wet wipe, mother. The disease caused by the innocuous inflammation of the external cervical spondylosis and the lumbar vertebrae, including the sputum of the spleen Inflammation, periarthritis, tennis money, myofascial syndrome, rheumatic sputum sputum, muscle cold bone cold, aseptic prostatitis, multiple inflammation, fire, neurodermatitis, tenosynovitis, lumbar muscle strain; due to aseptic disease ^ 'Sitting's neuralgia, heel pain, migraine, head-solid stomach pain, cancer pain, bronchial asthma; early aseptic femoral necrosis; and other diseases that are medically derogatory and belong to aseptic inflammation. 2 . The preparation method of the external preparation for treating aseptic inflammation as described in the scope of claims ir to 3, which is made by impregnating each of the 25 ml of the white-headed H. The wet tissue of 25 ml of the drug solution was adsorbed on the 0 cm2 administration area. I3. The method for treating aseptic inflammation according to claim 12, wherein the Pulsatilla sulphate liquid is treated with methanol, B, or water as a extracting agent, and contains whitehead, Or the natural plant or Chinese herbal medicine of Pulsatilla or edulis is prepared by leaching and collecting the extract of Pulsatilla in the sealing strip: the soaking package is cold soaked and hot dip. 1 4 · Aseptic treatment of aseptic inflammation as described in claim 3 Page 27 i27742 〇External T-patent —— _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ (4) The method for preparing the coating agent 2 for treating aseptic inflammation according to the third aspect of the patent application, wherein the ethanol is immersed to the point I ΐ using 3 〇 5 〇% of the wave After the boiling point, add natural plants or Chinese herbal medicines to continue heating! 6 Loose leaching for 6 months or more to make the pulverizer liquid. A method for producing a sputum for treating aseptic inflammation according to the above-mentioned claim 1, wherein the glucocorticol solution is prepared by using ethanol. The preparation method for the treatment of aseptic inflammation as described in claim 13 of the patent application scope, wherein the pulverizer is extracted with ethanol and the pulverizer liquid described in the night. The other four kinds of snoring diseases, such as the white preparation method for treating aseptic bismuth, as described in Item 1-3 of the scope of the patent application, will contain 12.5 mg of whitehead I 9 and Yusu liquid per 25 ml to prepare a topical liniment or Coating agent. For the treatment of aseptic inflammation of alcohol, ethanol or:::: 2, the pulverizer liquid is any Tiantie II using hyperthyroidism. Method for preparing a therapeutic external preparation as described in claim 19, which is prepared by containing a pulverizer or a pulverizer or a hair immersion extract , 1 ^ extract of innocuous inflammation, cold soak for 8 months to i + make / (a) Chen of the ethanol for # 1U months or more to make the Pulsatilla liquid. Shu Han Page 28 1277420 _ Case No. 90 Π 5998 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 50% of ethanol was used as a leaching agent, and after heating to the boiling point, natural plants or Chinese herbal medicines were added to continue to heat to the boiling point, and the pulverized liquid was prepared by sealing leaching for more than 6 months. A method for producing an external preparation for treating aseptic inflammation according to the invention of claim 18, wherein the pulverizer is prepared by using ethanol to prepare the pulverizer. The method for producing an external preparation for treating aseptic inflammation according to the above-mentioned claim, wherein the extract of the pulverizer is prepared into the pulmonale liquid by using ethanol. 5163-4131-PF2.ptc Page 29