TWI235671B - Method of estimating elimination of microorganisms and apparatus for estimating elimination of microorganisms - Google Patents

Abstract

It is intended to estimate the antimicrobial effect of a sterilization treatment whereby microorganisms are irradiated with particles. Microorganisms are introduced into the space in a container (8) and then irradiated with particles (7) for sterilization. After the completion of the irradiation with the particles (7), the microorganisms are collected into a collection container (6) and measured to thereby estimate the effect. The microorganisms being sterilized may be one or more members selected form the group consisting of bacteria, fungi, viruses and allergens. As the particles, use can be made of cations, anions, a gas containing a mixture of cations with anions, charged particles such as alpha- and beta-rays, various plasmatic gas ions, ozone and radical particles, drug particles and so on.

Description

1235671 (1) 发明 Description of the invention [Technical field to which the invention belongs] The present invention relates to a method for evaluating the removal of microorganisms and a device for evaluating the removal of microorganisms for evaluating the sterilization effect of space planktonic microorganisms [Previous technology] In recent years, with the The high airtightness of the living environment removes planktonic microorganisms in the air, which is harmful to the human body, and the requirements for a healthy and fast life are gradually increasing. In response to this demand, we have developed filters with various antibacterial agents. [Summary of the Invention] Problems to be Solved by the Invention However, because the above-mentioned filter attracts the air in the space and filters microorganisms in the air, after long-term use, the maintenance of the exchange filter is indispensable, and filtering The characteristics of the device are not sufficient, and satisfactory performance cannot be obtained, which is not enough as a way to remove microorganisms. Therefore, with regard to the usual evaluation of the removal of planktonic microorganisms, air containing microorganisms was passed through the filter, and the number of microorganisms filtered by the filter was measured. In this method, the concentration of planktonic microorganisms in the target space cannot be measured. However, there is a method for removing microorganisms by sterilizing the microorganisms by irradiating particles such as ionized ions, and measuring the removal ability of the microorganisms by sterilizing treatment in this way has never been performed before. Here, the present invention aims to provide microbes, especially viruses, with sterilizing treatment particles, especially ionic particles formed by positive and negative ions. • Ί (2) 1235671 is shot on microorganisms to evaluate their bactericidal effect. A method for evaluating microorganism removal and a device for evaluating microorganism removal using the method are intended. Means for solving the problem In order to solve the above-mentioned problem, the present invention is a method for evaluating the removal of microorganisms by supplying microorganisms to the internal space of the container, and simultaneously irradiating particles formed by positive and negative ions used for sterilizing the microorganisms. After the particles are irradiated, It is characterized by 'taken microorganisms' to carry out the determination of the taken microorganisms. In addition, "the present invention is a method for evaluating virus removal, supplying viruses as microorganisms to the container internal space" and irradiating particles for sterilizing the virus. After the particles are irradiated, the virus is collected and the virus is measured. According to this method, after the above particles are irradiated in the container's internal space, microorganisms are used to perform the measurement. Therefore, the ability to sterilize microorganisms by irradiating the particles can be evaluated, and various conditions for irradiating the particles can be quantitatively evaluated. Therefore, in the method for evaluating the removal of microorganisms, after the irradiation of the particles, the measurement of the microorganisms is performed, and further, the microorganisms are supplied under the same conditions as when the particles are irradiated to sterilize the microorganisms, and the particles are not irradiated, so that After the microorganisms have naturally decayed, the microorganisms are taken and the determination of the taken microorganisms is performed. That is, the invention irradiates the above-mentioned particles for a certain period of time to sterilize the microorganisms, and supplies the microorganisms under the same conditions as when the microorganisms are sterilized, to irradiate the particles for the same time as to irradiate the particles to make the microorganisms natural After the decline, the microorganisms were taken and the determination of the taken -8- (3) 1235671 microorganisms was performed. Therefore, the microorganisms that were sterilized by irradiating the above-mentioned particles and the microorganisms that were naturally degraded without the relevant sterilization treatment were measured, and the results were compared. Based on the comparison between the ability of the microorganisms that were irradiated with the above-mentioned particles to sterilize and the natural decay, A relative assessment can be made. The measurement of the microorganism is the measurement of the concentration of the microorganism, the measurement of the cell infection rate, or the measurement of the allergic reaction, and the removal of microorganisms can be evaluated. In addition, the measurement of the microorganisms collected as described above can be used to measure the change over time of the irradiation time of the particles. Based on this, the elapsed time of the ability to sterilize the microorganism can be quantitatively evaluated. In addition, for measuring the microorganisms collected as described above, the concentration dependence of the particles can also be measured. Based on this, the concentration dependence of the ability to sterilize the microorganisms can be quantitatively evaluated. The supply of microorganisms to the inner space of the container is a solution in which the microorganisms are dispersed and sprayed in a mist form. Accordingly, the microorganisms can be easily supplied into the container, and the microorganisms can be easily sterilized. Secondly, when the relevant microorganisms are sprayed in the form of a mist, they can be evaluated by the present invention. In addition, the above evaluation method can be used for cell culture of microorganisms, erythrocyte agglutination reaction of microorganisms, or allergic reactions of microorganisms. Accordingly, the activity or concentration of microorganisms can be evaluated. In addition, as the particles for sterilizing the microorganisms described above, any kind of gas generated by air discharge, radiation irradiation in the air, and Lenard effect can be used. (4) 1235671 In addition, as the particles for sterilizing the microorganisms described above, radiation, X-rays, 7-rays, or electromagnetic waves can be used. In addition, as the particles for sterilizing the microorganism, positive and / or negative ions may be used. Here, the reason why the sterilizing microorganisms can be sterilized by using positive and negative ions as special particles for sterilizing microorganisms is as follows. That is, the ionization phenomenon caused by discharge in the air causes positive and negative ions to generate H + (H20) η, which is the most stable, and zero plant (Η20) η, which is the negative ion. When these ions are generated, hydrogen peroxide Η 202 or radical · 0 活性, which is an active source due to a chemical reaction, because the Η 202 or free radical · 0 Η exhibits extremely strong activity, which can be sterilized to remove airborne microorganisms in the air. 〇 In addition, as the particles for sterilizing the microorganisms, a gas mainly composed of either positive or negative ions can be used. In this case, according to the charge of the above ions, the electric effect on the microorganism is the destruction of microbial cells or the destruction of surface proteins, and the effect of generating bactericidal effect can be obtained.

C In addition, the sterilization treatment may be performed by irradiating the medicament particles by using a medicament for sterilizing the microorganisms. In the case of a sterilization treatment using a chemical agent, the particles can be supplied in a simpler device as compared with the case of ionization or ozone. Therefore, the ability of related agents to sterilize microorganisms can also be evaluated. In addition, as the microorganism to be subjected to the sterilization treatment, the microorganism may be at least one kind or a combination of two or more members selected from the group consisting of bacteria, fungi, viruses, and allergens, and various microorganisms may be used as addition to the present invention. -(5) 1235671 Subjects to be evaluated. When the microorganisms are supplied to the inner space of the container, the inner space of the container can be stirred from the lower side with respect to the microorganisms supplied in the container. Accordingly, the supply of the microorganisms into the container can prevent the microorganisms from naturally precipitating due to the weight of the microorganisms, and can effectively sterilize by irradiating the particles. In addition, when stirring, it can also be the evaluation target of the present invention. In addition, the present invention provides a device for realizing the above-mentioned evaluation of microorganism removal, comprising an internal space capable of supplying microorganisms, a container for sterilizing the microorganism, and a microorganism supply means for supplying microorganisms to the internal space of the container, And the microorganisms removal means for supplying the particles for sterilizing the microorganisms in the above container internal space, and after the microorganisms are sterilized by the microorganisms removing means, the microorganisms are taken by the microorganisms and the microorganisms are measured by the microorganisms Apparatus for evaluation to evaluate the removal of microorganisms. According to the apparatus for evaluating the removal of microorganisms, the above-mentioned microorganism removal means irradiates the above-mentioned particles for sterilization of the microorganisms, and then the microorganisms are taken by the above-mentioned microorganisms taking means, and the measured microorganisms are measured. Based on the measurement, the above-mentioned microorganisms removing means can be evaluated Ability to sterilize microorganisms. In addition, various conditions for sterilizing microorganisms by irradiating particles in accordance with the above-mentioned microorganism removal means can be quantitatively evaluated. As a specific form, the microbial supply means, the microbial removal means, and the microbial take means are connected to the air passage containing the microorganisms, and are arranged in order from the upstream side to the downstream side. According to this, a series of steps of microbial supply, microbial removal, and microbial collection can be performed smoothly. At this time, if a microbial supply means and a microbial take-up means are used to form a wind tunnel containing an air passage containing microorganisms, and the microbial removal means is arranged inside the wind tunnel, it can be contained in the restricted wind tunnel. Supply, removal and collection of air from microorganisms. The microorganism removing means and the microorganism taking means may be arranged in a region other than a region directly below the microorganism supplying means. According to such a configuration, since the gaseous particulate matter falls vertically below and around the mist released by the above-mentioned microorganism supply means, the above-mentioned microorganism removing means and the above-mentioned microorganism taking means are not polluted by the above-mentioned falling substances. , Which can improve the reliability of the evaluation device. In order to obtain this effect, it is important that the microorganism removal means and the microorganism removal means are not disposed vertically below the microorganism supply means, for example, the microorganism removal means and the microorganism removal means are arranged horizontally, or the microorganism supply means This effect can be obtained by a deviation position vertically below, or by disposing the above-mentioned microorganism removal means and the above-mentioned microorganism taking means in an oblique direction. In addition, in the present invention, it is possible to arrange a device for evaluating the removal of microorganisms in other containers that can cover the above-mentioned container on the outer side of the container. With such a device structure, the microorganisms leaked out of the above-mentioned container, or the particulate matter that is not gasified can be shielded by the above-mentioned other containers, and it is difficult to leak from the outside. In addition, the apparatus for evaluating the removal of microorganisms may be provided with a stirring means for stirring the microorganisms supplied from below in the internal space of the container. -12 · (7) 1235671 Accordingly, when the microorganisms are supplied into the container by the microorganism supply means described above, the microorganisms are prevented from naturally precipitating due to their own weight, and the sterilization means by the microorganism removal means can be effectively performed. In addition, the apparatus for evaluating the removal of microorganisms may be configured to supply microorganisms by the microorganism supply means, so that the solution in which the microorganisms are dispersed is atomized, and sprayed on the inner space of the container. In addition, in the apparatus for evaluating the removal of microorganisms, the particles used for sterilizing the microorganisms may be constituted by the release of gas generated by any of air discharge, radiation in the air, and Lener effect (L e n a r d e f f e c t). In addition, in the apparatus for evaluating the removal of microorganisms, particles for sterilizing the microorganisms may be any of radiation, X-rays, r-rays, or electromagnetic waves, and may be constructed by emitting these. In the apparatus for evaluating microorganism removal, the microorganism removal means may be configured to irradiate positive and / or negative ions as particles for sterilization treatment of microorganisms. In the apparatus for evaluating removal of microorganisms, the means for removing microorganisms may be constituted by irradiating pharmaceutical particles as particles for sterilization treatment of microorganisms. Embodiments of the Invention The following describes the embodiments of the present invention. <First Embodiment> First, an apparatus for evaluating the removal of microorganisms capable of carrying out the method of the present invention will be described. Fig. 1 is a schematic diagram showing the evaluation of an example of a device for removing microorganisms. (8) 1235671 A schematic diagram of a device 10 for removing microorganisms. The apparatus 10 for evaluating the removal of microorganisms is provided with a container 8, a microorganism injection tube constituting a microorganism supply means 5, an ion generating device constituting a microorganism removal means 1, a microorganism acquisition tube 3 constituting a microorganism acquisition means, and a microorganism extractor 6. The container 8 has a structure in which the internal space blocks the outside air, so that microorganisms can exist in the internal space and the microorganism can be sterilized. In addition, the container 8 can be arbitrarily adjusted with the temperature or humidity of the internal space by an air conditioning system or the like not shown in the drawing, and the environment for the microorganisms can be arbitrarily set. In addition, as shown in FIG. 1, the container 8 has a shape in which the size in the height direction is larger than the size in the horizontal direction. Accordingly, since the volume of the space in the acquisition container 8 can be increased, the processing capacity of the apparatus 10 for evaluating microorganism removal can be increased. The microorganism injection tube 5 is provided at a predetermined position of the container 8. Via the microorganism injection tube 5, the microorganisms can be supplied to the internal space of the container 8, and the microorganisms can float in the internal space of the container 8. The microorganism injection tube 5 is fed with microorganisms from a microorganism supply source not particularly shown in Fig. 1. Therefore, the microorganisms are injected into the container 8 from the microorganisms injection port 5a in the container 8 through the microorganisms injection pipe 5. Regarding the injecting of microorganisms into the container 8 from the microorganism injecting tube 5, a microbial monomer may be injected, or a solution in which the microorganisms are dispersed may be sprayed into the container. The ion generator 1 irradiates ions -14-(9) 1235671, which are particles for sterilizing microorganisms. The ion generating device 1 is installed in the container 8 and faces the microorganisms injected into the container 8 through the microorganism injection port 5a, and ions 7 are irradiated from the ion generating port 2. The ion generating device 1 is provided with an ion generating element inside thereof. When an AC voltage is applied between the electrodes of the ion generating element, ions 7 formed by positive ions and negative ions are generated due to an ionization phenomenon such as a discharge. Ions 7 generated by the discharge or the like of the related ion generating device 1 are not affected by the state of the gas pressure in the container 8. In addition, the intensity (concentration) of the ions 7 can be adjusted by adjusting the applied voltage to the ion generating element of the ion generating device 1. In the space inside the container 8, a microorganism collecting tube 3 for collecting microorganisms is provided. The take-up tube 3 is constituted by a portion arranged in a vertical direction along the height direction of the container 8 and a portion arranged in a horizontal direction of the container 8 as shown in FIG. 1. Next, a portion provided along the horizontal direction of the collecting tube 3 penetrates the side of the container 8 and extends to the outside of the container 8 and is connected to a microorganism collecting device 6 described later on the outside of the container 8. A microorganism collecting port 3a is formed at the upper end in the vertical direction of the collecting tube 3, and microorganisms in the container 8 are taken into the collecting tube 3 through the collecting port 3a. The microorganism collecting device 6 is arranged outside the container 8 and the collecting tube 3 constitutes a microorganism collecting device. The microorganisms collector 6 is adapted to suck the space in the container 8 through the microorganisms collection tube 3, and the microorganisms in the container 8 are taken into the collection tube 3 through the microorganisms collection port 3a and collected in the microorganisms collection device 6. -15-(10) 1235671 Contains a microbial sampler for collecting microorganisms. About microorganism collection The foamer is composed of microorganisms. A stirrer 4 is provided below the apparatus 10 for evaluating the removal of microorganisms. As the means for agitating the space of the agitating portion, a person who agitates the space by rotating air flow can be used. The stirrer 4 is provided to stir the contents of the container 8 naturally due to its own weight, which can effectively sterilize the ions 7 irradiated by the biological device 1. In particular, microorganisms are of a heavy quality, and a stirrer 4 is provided to prevent sterilization of the natural sedimentation 7. In addition, when the present invention is carried out, it is not always necessary to provide a stirrer 4, and the sterilization treatment can be performed for the reasons described above. The method using the above apparatus for evaluating microorganism removal can be carried out as described below. First, the injection port 5 injects a certain amount of microorganisms into the container 8 and the device 1 operates' to sterilize the photos of the injected microorganisms. Irradiate ions 7 6 for a certain period of time to take microorganisms. For the microorganisms taken, the bacteria container 6 can be measured, the gas extractor 6 can be used, or the solution can be used. As shown in Figure 1, when the internal fan of the container 4 is a stirring container 8 to make the surrounding space form a gas space, To prevent micro-organisms from floating in the ion generation range, when it can be effectively carried out, natural sinking is likely to occur. Ion can be effectively provided with a stirrer 4, but it is more effective to evaluate the ions 7 and 10. . Next, the ions are irradiated with ions 7, and the microorganisms are collected by the microorganisms. About the determination of microorganisms • 16- (11) 1235671 The number of microorganisms can also be cultured in a Petri dish with a predetermined culture medium and cultured for a certain period of time. This allows accurate measurement of the number of microorganisms collected. In addition, for the measurement of the number of microorganisms, the microorganisms on the above-mentioned culture dish can be observed with a microscope. In this way, using the apparatus 10 for evaluating the removal of microorganisms, the microorganisms collected by the microorganisms collector 6 can be measured, and the ability to sterilize the microorganisms when the ions 7 are irradiated can be evaluated. In addition, with respect to the microorganism removal evaluation using the apparatus 10 for evaluating microorganism removal described above, the following measurement and evaluation can also be performed. First, as described above, a certain amount of microorganisms are injected into the container 8 and ions 7 are irradiated to perform a sterilization treatment for a certain period of time. After that, the microorganisms are collected by the microorganisms collector 6 to measure the number of microorganisms of the collected microorganisms. Next, the same amount of microorganisms were injected into the container 8 under the same conditions as the sterilization treatment of the above-mentioned irradiated ion 7. Second, without irradiating the ions 7, the microorganisms naturally decay after the same time as the above-mentioned irradiating ions 7. Thereafter, the microorganisms are collected by the microorganism extractor 6 and the number of bacteria of the collected microorganisms is measured. Secondly, compared with the bacterial counts of the microorganisms taken after the above-mentioned sterilization treatment with ion 7 irradiation, compared with the bacterial counts of the microorganisms taken after the natural decay, the bacterial sterilization treatment capacity of the ion 7 with respect to the natural decay can be compared Assessment. In addition, regarding the measurement of the microorganisms collected by the microorganism extractor 6 described above, it is also possible to measure the change over time of the number of microorganisms with respect to the elapsed time from the start of irradiation with ions 7 or the elapsed time for the microorganisms to naturally decay. 17- (12 ) 1235671 In addition, when performing the above microorganism measurement, the mixer 4 is also used for stirring, and the measurement is performed without stirring. In addition, when the above-mentioned microorganism measurement is performed, the ion 7 strength of the microorganism is also measured, and the measurement of each of the strong microorganisms of the ion 7 is performed. Based on this, it is possible to evaluate the sterilization treatment ability of the corresponding ion 7 strong substance. <Second Embodiment> Next, a second embodiment of the evaluation microdevice according to the present invention will be described with reference to FIG. FIG. 2 is a device 20 for evaluating the removal of microorganisms according to the implementation type 2 of the device 20 for evaluating the removal of microorganisms. The device 20 is similar to the device 20 for evaluating the removal of microorganisms shown in FIG. 2. 18. 12. The take-up tube 13 and the microbial take-up device 6 constituting the micro-biology. That is, the micro-organism injecting tube 15 and the micro-organism removing means 12 'and the micro-organism taking means χ 3 and the micro-organism are connected to the air path containing the micro-organisms, and are constituted by the upstream side facing downward. Gu Yi 18 is that the internal space is to block the outside air from the existence of organisms in its internal space and can sterilize the microorganism. It is known that the container 18 adopts a height-type size smaller than the level. The structure diagram of the device for removing micro-organisms can be used to change the irradiation intensity. The container is provided with a capacity of 15, which constitutes a means for taking micro-organisms, a biological supply hand, and an ion-generating element collector. Dimensions in the direction from Fig. 2 -18- (13) 1235671 The microbial injection tube 15 is connected to the outside of the container 18 and is connected to the microbial sprayer 11 to feed the microorganisms from the microbial sprayer 11. The microbial sprayer 11 sends a microbial gas containing a certain concentration into the microbial injection pipe 15 at a certain speed. Next, the microorganism-containing gas system fed into the microorganism injection tube 15 from the microorganism sprayer 11 is injected into the container 18 through the microorganism injection port 15a in the container 18. Regarding the supply of microorganisms into the container 18 by the microbial sprayer 11, the microbial monomers in the air can be sent to the microbial injection tube 15, or the solution of dispersed microorganisms can be misted, and the spray can be sent to the microbial injection tube 15 °. Ion generation The element 12 is disposed on the bottom surface inside the container 18 other than the region directly below the microorganism injection tube 15. The ion generating element 12 is an ion generator 12a arranged in a certain approximately flat shape, and generates ions 7 formed by positive ions and negative ions. The ions 7 generated by the ion generating element 12 process the microorganisms injected by the microbial injection tube 15. The ion generating element 12 is the same as the ion generating element provided in the ion generating device i shown in FIG. 1 and generates ions 7 The operation is the same as that described for the ion generating device 1. The microbial collection tube 1 3 for collecting microorganisms is arranged outside the area vertically below the microbial injection tube 15. Along the horizontal direction, one end forms a microbial collection port 13 a toward the container 18, and the other end is in the container 18. The outside is connected to the microbe extractor 6. The microbial collector 6 arranged outside the container 1 8 is via the micro-biology -19 · (14) 1235671 material collection tube 13 to attract the space in the container 18, and the container 8 organisms are taken in through the microorganism collection port 13 a. Take inside the tube 13 and the microbial taker 6. As the microbial collector 6 for collecting microorganisms, a sampler can be used. The microorganism extractor 6 may be configured to collect microorganisms through a solution. Using the apparatus 20 for evaluating microorganism removal described above, the present invention can be implemented as described below. First, the microorganism is injected into the container 18 from the microorganism injection port 15. Next, the ion generating element 12 is irradiated with the ions 7 to the injected microorganisms to kill the microorganisms. After a certain period of time, the ion 7 is irradiated, and the microorganism is taken by the microorganism extractor 6. Then, the microorganism taken by the microorganism taker 6 is measured to determine the number of microorganisms taken. The selected microorganisms are cultured on a predetermined culture medium, and then cultured for a certain period of time. In addition, the measurement of the number of microorganisms can also be performed by observation with a microscope. Thus, the measurement uses the device 20 for evaluating the removal of microorganisms, and the microorganisms collected by the collector 6 can be used to evaluate the sterilization treatment ability of the object when the ion 7 is irradiated. In addition, the device 20 for evaluating the removal of microorganisms can be injected into the container 18 by the microorganism injection port 15 and the ions 7 of the photo-generating element 12 to sterilize the microorganisms. A series of micro-organisms formed by micro-organisms are taken into a certain operation by the method of taking a gas bubbler, and the micro-organisms are treated by bacteria. turn off . Regarding the dish, in addition to the micro-organisms in the micro-sheng according to the treatment of the ions after the -20, (15) 1235671, one of the approximate implementation pathways. Therefore, the apparatus 20 'for evaluating the removal of microorganisms does not need to consider the natural degradation of microorganisms in the container 18, and therefore, it is possible to perform the evaluation for removing high-concentration airborne microorganisms. In addition, the device 20 'for evaluating the removal of microorganisms can be miniaturized and can be evaluated in a confined space, so even harmful microorganisms can be evaluated. In addition, when the method of the present invention is performed using the apparatus 20 for evaluating microorganism removal, the following measurement and evaluation can be performed in the same manner as described when the apparatus 10 for evaluating microorganism removal shown in FIG. 1 is implemented. That is, the microorganisms supplied into the container 18 are naturally degraded without being irradiated with ions 7, and the ions 7 are irradiated for sterilization treatment, and the results of the measurement of the microorganisms collected by the collector 6 can be compared. In addition, as for the measurement of the collected microorganisms, the elapsed time from the start of irradiation of the ions 7 or the elapsed time for the microorganisms to naturally decay can also be measured over time. In addition, by changing the intensity of the ions 7 of the irradiated microorganisms, and measuring the microorganisms used for each intensity of the ions 7, the sterilization treatment ability of the microorganisms in accordance with the intensity of the ions 7 can be evaluated. In addition, the above description is described as an example of ions 7 formed by irradiating positive and negative ions of particles for sterilizing microorganisms. In addition, for particles used for sterilizing microorganisms, pharmaceutical particles can also be used. When using pharmaceutical particles, change the ion generating device 1 of the evaluation microorganism removal device 10 shown in FIG. 1 or the evaluation microorganism removal device shown in FIG. 2 • 21-(16) 1235671 Change the ion generating device 12 at 20 to The present invention can be implemented by means for spraying the medicine particles. When the above-mentioned pharmaceutical particles are used, as the medicine, alcohol or aldehyde medicines, antiviral agents, insecticides, and the like can be used. <Third embodiment> Next, a third embodiment of the apparatus for evaluating microorganism removal according to the present invention will be described with reference to Fig. 8. Fig. 8 is a schematic configuration diagram showing a third embodiment of the apparatus for evaluating the removal of microorganisms. Compared with the second embodiment, a wind tunnel and other containers are provided outside the closed inner container. That is, as shown in the figure, the apparatus 30 for evaluating microorganism removal of this type is provided with a container 18, a microorganism injection tube 15 constituting a microorganism supply means, an ion generating element 12 constituting a microorganism removal means, and Take the tube 13 and the microorganism taker 6. Next, inside the container 18, a space from the injection pipe 15 to the collection pipe 13 is provided with a wind tunnel 31. Inside the wind tunnel 31, an ion generating element 12 is arranged. The internal space of the container 18 is a structure that blocks outside air, and adopts a form in which the size in the height direction is smaller than the size in the horizontal direction. On the single side wall of the container 18, a gasket 32 is passed through, and the injection pipe 15 is led out from the outside into the container. In addition, on the side wall opposite to the injection pipe 15, a pipe 13 is led through the gasket 32 and led out. Into the container. The microorganism injection tube 15 is connected to the outside of the container 18 and is connected to the microorganism sprayer 11 and the microorganisms are fed by the microorganism sprayer 11. The microbial sprayer 11 sends a microbial gas containing a certain concentration into the microbial injection pipe 15 at a certain rate. Secondly, the microbe sprayer 11 feeds -22- (17) 1235671 The microbe-containing gas system of the microbe injection tube 15 is injected into the container 18 from the microbe injection port 15a in the container 18. In addition, at this time, the microbial monomer may be contained in the air and sent to the microbial injection pipe 15, or the solution in which the microorganisms are dispersed may be misted and sprayed into the microbial injection pipe 15. The wind tunnel 31 in the container 18 is formed in a cylindrical shape and is arranged approximately horizontally, and its two ends are arranged toward the injection pipe 15 and the taking pipe 13. The ion generating element 12 is disposed on the bottom surface on the slightly upstream side in the wind tunnel 31 outside the vertical downward region of the microorganism injection tube 15. The ion generating element 12 is an ion generating electrode arranged in a certain approximately flat shape, generating positive ions and negative ions, and sterilizing the microorganisms injected by the microorganism injection tube 15. The ion generating element 12 is the same as the ion generating element provided in the ion generating device 1 shown in FIG. 1, and the operation of generating the ion 7 is the same as the description of the ion generating device 1. The microbial collection tube 1 3 for collecting microorganisms is arranged horizontally on the opposite side of the microbial injection tube 15. One end of the collection tube 1 3 a forms a microbial collection port 1 3 a toward the inside of the container 18, and the other end is outside the container 18. , Is connected to the microbial collector 6. The microbial extractor 6 stores the foaming liquid inside, and the microorganisms sinking in the foaming liquid take the end portion of the tube 13 to foam the taken-in air and recover it. Secondly, the spray test systems of the container 18, the microbial sprayer 11 and the collector 6 are all covered by other containers 35. In addition, in this embodiment, the mist system -23 · (18) 1235671 released from the injection port 15a is sprayed inside the wind tunnel 31, and there is a little space between the injection port 15a and the wind tunnel 31. The water drops fell on the container 18. In addition, the gas released from the injection port 15a has a certain speed depending on the spray, and at this speed, passes through the wind tunnel 31 at the direction shown by the arrow in Fig. 8. According to this mechanism, the particles formed by the ions emitted from the discharge electrodes of the ion generating element 12 react with the microorganisms, and when they reach the collector 6, the ion removal effect of the microorganisms can be confirmed. In addition, the method for evaluating microorganisms is not particularly limited, and all evaluation methods such as evaluation of agar medium, evaluation of cell culture, erythrocyte agglutination reaction, allergic reaction of organisms or cells, and microscopic observation can be used. In addition, in the mist containing microorganisms, the components that have not been vaporized and rapidly dropped into a droplet shape or the unused microorganism components are accumulated in the wind tunnel 3 1 '. Although the container 18 contained therein has a structure that is difficult to leak out, However, because the outer side is covered with other containers 35, it is less likely to affect humans and the like located outside. Therefore, even if the wind tunnel 31 and the container 18 are not completely closed, the incidence of accidents such as biohazard can be greatly reduced. In addition, the shielding effect 'of the other container 35 can prevent unwanted contamination from being mixed in from the outside, so that an effect of improving the accuracy of evaluation can be obtained. <Fourth implementation type> Fig. 9 is a schematic configuration diagram showing a device for evaluating the removal of planktonic microorganisms. In the evaluation device shown in the third sinus application type, the particle discharge part is replaced with a needle discharge device. . -24 · (19) 1235671 That is, ‘this embodiment replaces the ion generating element 12 of the third embodiment, and a needle discharge device 40 is installed. The needle-type discharge device 40 is located in the wind tunnel 31 and is composed of a needle-type discharge electrode 40a arranged on the upstream side region and an anti-plate electrode 40b arranged on the opposite side. Since the other configuration is the same as that of the third embodiment, 5 its explanation is omitted. · In this embodiment, a few kV is applied to the needle electrode 40a. · When a positive or negative high voltage is applied, a discharge is caused around the tip of the needle, and the positive or negative charged ions that are ionic components are mainly emitted. Of gas. · Based on the above composition, a positive or negative ion-based gas is emitted, and the microbial-containing mist emitted by the microbial injection tube 15 is irradiated to sterilize and remove microorganisms. Therefore, the microorganism removal evaluation test can be performed. In addition, in the present embodiment, the released particles 7 are not limited to ionomers. Free radicals, ozone, or other particles with bactericidal effects can be used. <Fifth Embodiment> φ Figure 10 represents evaluation A schematic structural diagram of a device for removing plankton virus. In this embodiment, in the evaluation device shown in the third embodiment, the particle emission portion formed by the ion generating element 12 is replaced with a radical emission mechanism of an ultraviolet lamp and a catalyst. That is, the present embodiment replaces the ion generating element 12 of the third embodiment, and is provided with a radical emission mechanism 50. The free radical emission mechanism 50 is located in the upstream side region of the wind tunnel 31, and is composed of an ultraviolet lamp 50a arranged at the center and a catalyst 50b arranged on the opposite side of the ultraviolet lamp 50a. Since • 25 · (20) 1235671 is the same as the third embodiment, the description is omitted. The catalyst 5 Ob is made of a material including platinum, gold, titanium oxide, and the like. Because of the radiation energy radiated from the ultraviolet lamp 50a, the energy is used to generate free radicals, which has the effect of releasing the generated free radicals into space. In addition, the catalyst 50b is not limited to those containing materials such as platinum, gold, titanium oxide, and the like. Of course, as long as an active gas can be emitted, the same removal evaluation test can be realized. In addition, in this embodiment, the device for removing the catalyst 5 Ob can also be evaluated. At this time, the photons 7 of the fine particles emitted from the outside light 50a can sterilize the microorganisms existing in the space. In addition, the lamp 50a uses this example to show that it emits radiation. As its radiation, it has light with an energy of 5eV to 20eV, which has excellent sterilization performance. The radiation containing this light is suitable for this evaluation device, and the evaluation result obtained can be expected. In addition, as the above-mentioned radiation, an X-ray or 7-ray-emitting element may be disposed at the position of the ultraviolet lamp 50a, and the test can be performed in the same manner. In addition, 'the evaluation method and the evaluation device are effective using the above-mentioned test using the radiation. Of course, other particles can also be used, such as infrared rays and other hot rays and visible light, which can be evaluated according to the present invention. At this time, in principle, it can be considered that the sterilization performance is controlled by heat. When a strong hotline and visible light are required, it is desirable to provide a heat release mechanism or a light shielding plate. In addition, when radiation is used, electromagnetic waves from 2 GHz to 200 GHz can be sterilized. At this time, a catalyst is not necessarily required, and the constituent elements of microorganisms can absorb the energy of electromagnetic waves, and the microorganisms can be destroyed to a molecular level. Because -26- (21) 1235671, the required electromagnetic wave 'can easily be, for example, an electromagnetic wave of about 2.45 GHz, and for example, an electromagnetic wave of 2 GHz can be used. In addition, in the frequency above 2GHz, it is considered that it is easy to be an electromagnetic high-frequency element of a higher frequency range absorbed by the microbial element. As a possible frequency, 120 GHz is a frequency that can be used for sterilization. In addition, in the device shown in FIG. 10, in the ultraviolet light; the device can be provided with an electromagnetic wave emitting element or an optical waveguide such as an optical fiber. <Target microorganism> In addition, the bacteria, bacteria, viruses, and allergen-inducing substances (proteins, etc.) to be subjected to the sterilization treatment in the present invention can be used in the practice of the present invention, and monomers of these fungi, bacteria, and disease substances can be used, Use plurals in combination. In addition, the virus is incorporated into the invention of general microorganisms, and the effect of suppressing its proliferation is sterilized or removed. In general, the effect of inhibiting the proliferation of the virus is to use inactivated words for viruses. In this specification, inactivated words can also be used for sterilization or removal. In addition, similarly, regarding the effect of allergen substances on inducing allergic reactions in the body and the like, the general effect of sterilization or removal is replaced by the statement that the above-mentioned effect can be inactivated, and the right-to-short-wavelength proteins that are not absorbed in this report The wave is a candidate, and it can be considered that the 50a position element and the electric heating system include fungi. Secondly, the scope of drugs and allergens is expressed in this sentence, so the sentence is replaced by the sentence of suppression in the Ming. In the book, also -27- (22) 1235671 can use inactivated sentences to take a _ to replace or remove the ffl of the allergen substance

Sentence C [Embodiment] Example [Example 1] As Example 1 ', the following conditions were implemented. As for the evaluation of the removal of microorganisms, an apparatus i 0 for evaluating the removal of microorganisms as shown in FIG. 1 was used. The container 8 containing 10% of the microorganisms to be evaluated for this evaluation is the one having an internal space of 2.0 m in length and 2.5 m in height and 2.7 m in height. Secondly, the temperature of the ambient air inside the container 8 is 25 ° C, and the relative humidity is 42%. The internal space of the container 8 is stirred by the mixer 4. The stirring system with the mixer 4 was performed with an air volume of 4 m3 / min. Use of coliform bacteria as microorganisms. Regarding the supply of this coliform in the container 8, the microorganism injection port 5a is supplied in a mist form. Secondly, the coliforms are dispersed in the container 8 at a concentration of about 500 to 1,500 / m 3. The collector 6 is configured using a Biotest Hyton RCS gas sampler. When collecting microorganisms with a gas sampler, the collection was performed at 40 L / min for 4 minutes. Next, the ions 7 formed by the positive ions and the negative ions generated by the ion generating device 1 are irradiated. In this embodiment 1, various ion concentrations are changed, and each ion concentration is irradiated with the ions 7 for one hour to perform a sterilization treatment. The ion concentration is the distance of 10 cm from the ion sending part of the ion generating device 1 (ion generating port -28-(23) 1235671 2). Next, "coelenteritis was supplied to Guyi: Neihou" under the above-mentioned conditions, and the ions 7 were irradiated with the ion concentration for 1 hour, and then the coliform bacteria were collected by the above-mentioned gas sampler, and the number of the coliform bacteria was measured. Next, the ion concentration of various ions 7 is changed, and the measurement of each ion concentration is repeated. FIG. 3 shows the measurement results of Example 1. FIG. In FIG. 3, it corresponds to the ion concentration of ions 7 expressed in logarithm (number / cm: In addition, in FIG. 3, the vertical axis system corresponds to the phytoplankton residual rate (%). The phytoplankton residual rate is after the ion 7 is irradiated. The number of remaining bacteria that have not been sterilized is expressed in terms of ratio. As shown in FIG. 3, when the ion concentration emitted from the ion generator 1 is increased, it is confirmed that the residual rate of airborne bacteria in the air is reduced. If the number of positive and negative ions is 10,000 When the rate is above / cm3, the residual rate is also confirmed to be extremely low. Secondly, the general indoor ion concentration is 500 to 1,500 /, as a general standard for effective removal of microorganisms, it is considered to send positive and negative ion concentrations above / / cm3. It is appropriate. [Example 2] As Example 2, it was implemented under the following conditions. The micro-removal evaluation was performed using the apparatus for evaluating microorganism removal shown in Fig. 1. The container 8 for evaluating the micro-removal device 10 was used. The internal dimensions are 2.0m in length, 2.5m in width, and 2.7m in height. Be sure to take the measurement and change the horizontal axis) °. In addition to the positive and negative values, the rate of rapid decline is between cm3 and 10,000 creatures, and between -29 and (24) 1235671. Second, the ambient air temperature inside the container 8 is 251, and the relative humidity is 42%. The internal space of the container 8 is stirred by the mixer 4. The stirring with the mixer 4 was performed with an air volume of 4 m 3 / in. Use of coliform bacteria as microorganisms. Regarding the supply of this coliform in the container 8, the microorganism injection port 5a is supplied in a mist form. Next, the coliforms were dispersed in the container 8 at a concentration of about 1,000 pieces / m3. In addition, the collector 6 is configured using a Biotest Hyton RCS gas sampler. When the microorganism was collected by the gas sampler, it was collected at 40 L / min 'for 4 minutes. Next, the ions 7 irradiated with the ion generating device 1 are sent out, and the ions 7 not irradiated with the ion generating device 1 are not sent out. The ions are naturally decayed, and they are collected by the above-mentioned gas sampler. When the ions are sent out, the ion concentration is a distance of 10 cm from the ion sending part, and the positive and negative ions are 50,000 / cm3, respectively. Next, when the above-mentioned ion delivery and non-ion delivery are performed respectively, coliform bacteria are collected every 15 minutes by the above-mentioned gas sampler, and the number of bacteria of the collected coliform bacteria is measured. Fig. 4 shows the measurement results of Example 2 and shows the change with time of the residual rate (%) of the planktonic bacteria. In FIG. 4, the horizontal axis system corresponds to the elapsed time, and the vertical axis system corresponds to the phytoplankton residual rate (%) as in FIG. 3. When the ion is not sent out, the residual bacteria decay rate after natural lapse of 1 hour is 80%. On the other hand, when the ion was sent out, the residual bacteria rate after 10 hours had passed was 10%. Regarding the above measurement, -30 · (25) 1235671 is a general standard for judging the effect of removing microorganisms. If the accuracy of the microorganisms and the accuracy of concentration measurement are considered, it is considered that the residual rate of natural decay is 10%. Differences are intentional. In addition, if the accuracy of the test is considered, the natural decay when the ions are not sent out, and the test conditions of 50% or more of residual bacteria rate after 1 hour are suitable. / Figure 5 shows the photos of coliforms taken 15 minutes after the ion release and non-ion release ~. FIG. 5 (a) is a person who performs ion emission, and FIG. 5 (b) is a person who does not perform ion emission. In addition, regarding the photography of the coliforms shown in FIG. 5, the coliforms taken in each of the above cases were cultured on an agar medium at 34 ° C and 10.0% RH for 48 hours, and then photographed. In addition, in FIG. 5, the size of the petri dish is 9 cm. ~ When ion delivery was performed, as shown in Fig. 5 (a), no colony formation of coliform bacteria was observed. On the other hand, when ion delivery was not performed, as shown in FIG. 5 (b), colonies of coliform bacteria were observed. It can be seen from the results shown in FIG. 5 that sterilization is possible with ions. &lt; 1 [Example 3] Example 3 was implemented under the following conditions. As for the evaluation of the removal of microorganisms, an apparatus for evaluating the removal of microorganisms shown in FIG. 1 was used. The container 8 of the apparatus for evaluating the removal of microorganisms is a container having a size of 2.0m in length, 2.5m in width, and 2.7m in height. Second, the ambient air temperature inside the container 8 is 25 ° C, and the relative humidity is 42%. In addition, in the third embodiment, as described in the invention below, stirring is performed in the container 8. • 31-(26) 1235671 泮 In comparison with non-stirring, the space in the stirring container 8 is stirred with a mixer 4 with an air volume of 4 m3 / min. As a microorganism, Cladosporium, a fungus, was used. When the black mold is supplied into the container 8, the black mold is supplied from the microorganism injection port 5a in a mist state. Next, the concentration of black mold was dispersed in the container 8 at about 1,000 pieces / m 3. ^ The collector 6 is constructed using a Biotest Hyton RCS gas sampler. When collecting microorganisms with a gas sampler, the collection was performed at 40 L / φ per minute for 4 minutes. Next, with the agitator 4 and without the agitator 4, the above-mentioned gas sampler was used to collect airborne bacteria in the air every 15 minutes, and the number of bacteria was measured. ‘FIG. 6 shows the measurement results of Example 3, and shows the change with time of the residual rate (%) of planktonic fungi in the air that naturally decays with or without stirring. In FIG. 6, the horizontal axis system corresponds to the elapsed time, and the vertical axis system corresponds to the phytoplankton residual rate (%) as in FIG. 3. · When stirring is not performed, after 45 minutes, the residual rate of bacteria whose detection limit is 12%. On the other hand, during the stirring, the residual rate of bacteria was 80% because of natural decay after 1 hour. ^ From the above results, adding agitation means can suppress the natural fall of bacteria, and it is easy to evaluate and remove planktonic microorganisms. In particular, when the mass of bacteria is large, stirring is effective. [Example 4;] -32- (27) 1235671 As Example 4, it was implemented under the following conditions. Regarding the microbial removal delta evaluation, the apparatus 10 for evaluating microbial removal shown in Fig. 1 was used. The container 8 of the apparatus 10 for evaluating the removal of microorganisms is a person using an internal space with a size of 2.0 m in length, 2.5 m in width, and a height of a blade. Secondly, the temperature of the ambient air in the container 8 is 25 ° C, and the relative humidity is 42%. The internal space of the container 8 is stirred by the mixer 4. The stirring system with the mixer 4 was performed with an air volume of 4 m 3 / m i η. Cladosporium is used as a microorganism. When supplying the black mold to the container 8, the microorganism injection port 5a is supplied in a mist form. Secondly, the concentration of black mold was dispersed in the container 8 at about 15 000 pieces / m3. The collector 6 is configured using a Biotest Hyton RCS gas sampler. When collecting microorganisms with a gas sampler, the collection was performed at 40 L / min for 4 minutes. Next, "the ions 7 irradiated to the ion generating device 1 are sent out, and the ions 7 not irradiated to the ion generating device 1 are not sent out, so that they naturally decay." When the ions are sent out, the ion concentration is a distance of 10 cm from the ion sending part, and the positive and negative ions are 50,000 / cm3, respectively. Next, when the ions are sent out and the ions are not sent out, a black mold is taken every 15 minutes with the gas sampler described above, and the number of bacteria taken is measured. Fig. 7 shows the measurement results of Example 4 and shows the change with time of the residual rate (%) of planktonic bacteria. In Fig. 7, the horizontal axis corresponds to the elapsed time, and the vertical axis of 33- (28) 1235671 corresponds to the residual rate of phytoplankton (%) in the same manner as in Fig. 3. ° When the ion is not sent, the natural decay rate after 1 hour has elapsed. The residual rate of bacteria was 75%. On the other hand, the residual bacteria rate after 5 hours passed when the ion was fed out was 10%. Regarding the above measurement, as a general standard for judging whether the effect of removing microorganisms is effective, if the accuracy of measuring microorganisms and the accuracy of measuring the concentration are considered, a difference of 10% from the residual rate of natural decline is considered to be an intentional difference. In addition, if the accuracy of the test is taken into consideration, the natural decay when the ions are not sent out, and the test conditions for a bacteria residual rate of 50% or more after 1 hour are suitable. [Example 5] Example 5 was implemented under the following conditions. As for the evaluation of the removal of microorganisms, the apparatus 20 for evaluating the removal of microorganisms shown in Fig. 2 was used. The container 18 of the apparatus 20 for evaluating the removal of microorganisms was formed by using a quadrangular column with a bottom edge size of 8cxn and a length of 30 cm. Secondly, the temperature of the ambient air system in the container 8 is 25 ° C and the relative humidity is 50%. Poliovirus (P 0 1 i 0 v) · r u S) is used as the treatment microorganism. Secondly, an aqueous solution in which tens of thousands of polioviruses are dispersed per cc is mixed with air to form a mist, and it is supplied to the injection port 1 5 a at a rate of 0 · 1 cc / mi η and a wind speed of 1 · 6 m / sec. Inside the container 18. In addition, regarding the space in which the above-mentioned virus was irradiated with ions 7 and the ions were sent from the ion generating element 12 in a sterilizing treatment] space, the positive -34-(29) 1235671 negative ions were 100,000 / cm3, respectively. In addition, the poliomyelitis virus after the sterilization treatment by irradiating the above-mentioned ions 7 was collected in the collector 6 by a solution foamer to capture the virus. Next, the poliomyelitis virus sterilized by irradiating the ion 7 was collected on the collector 6, and when the number of bacteria was measured, the virus removal rate was 78%. [Example 6] Example 6 was performed under the following conditions. FIG. 8 is a schematic configuration diagram of a device for evaluating the removal of planktonic viruses in this embodiment, and FIG. 11 is a graph showing the frequency of cellular infection of influenza virus based on ion concentration, and FIG. 12 is a diagram of kerax virus based on ion concentration (Coxsakie virus) cell infection frequency chart, Figure 13 shows the ion infection-based polio virus frequency chart. Fig. 14 is a mass spectrum chart showing positive ions and negative ions generated by the ion generating element. Fig. 15 is a flowchart of an evaluation test for comparison when the ion generating element is not operated and when the ion generating element is operated. In this embodiment, as shown in the flowchart of Fig. 15, after preparing a solution containing a microorganism, the test device is used to spray the solution into a space to take in air. In addition, after spraying, the sprayed microbe-containing air is subjected to a step of releasing particles having a bactericidal effect and causing them to act. In addition, tests were performed when the particles were discharged and when the particles were not discharged. The solution used in the above method is, for example, the control method, the red blood cell agglutination reaction, etc., to evaluate the concentration of microorganisms or the activity, etc., to evaluate the effect of sterilization or inactivation, and to compare the effect of particles with and without them This confirms the effect of -35- (30) 1235671 fixed particles. In addition, by changing the concentration of particles or the action time of particles, it is possible to investigate the irradiation time dependence or particle concentration dependence on the degree of sterilization or inactivation. This embodiment uses an apparatus 30 for evaluating the removal of microorganisms as shown in FIG. The ion generating element 12 is a flat surface discharge element having a length of 37 mm and a width of 15 mm. The application of positive and negative high voltages between the electrodes causes surface discharge on the surface of the electrode part. The discharge plasma at atmospheric pressure generates positive and negative ions. The ion generating element is fixed at one end of an acrylic cylindrical container 31 having an inner diameter of 55 mm and a length of 200 mm. A virus sprayer 11 is installed on one side of the container 18 or more, and the other side is provided with The pick-up device 6 is used for virus liquid recovery. Influenza was inoculated into the allantoic membrane cavity of a developing egg, and cultured in an incubator, then the allantoic fluid was taken as the test virus fluid. 10 ml of the test virus solution was put out of a glass sprayer (virus solution sprayer 11) and connected to one end of a container 18. The other end of the container 18 is connected to a glass dust detector (taker 6) containing 10 ml of PBS (-). The discharge pressure of the compressed air from the gas sampler in the sprayer was adjusted to 0.48 hPa by the gauge pressure, and the virus was sprayed into the wind tunnel 31 in the container 18 through the injection port. The spray volume was 3.0 ml (spray flow rate: 0.1 μm1 / minx spray time: 3 Om in). At this time, the ion generating element 12 was controlled to be in a non-operation state, and the ion concentration was 200,000 / cm3, 100,000 / cm3. And 50,000 pieces / cm3. -36- (31) 1235671 The dust tester sucks the air in the test device at a suction flow rate of 10L per minute for 30 minutes. The PBS (-) in the air in the capture test device attracted by the dust tester is the test solution. Influenza viruses are measured using the control method of main darby canine kidney (MDCK) cells. In addition, the Quesha virus and polio virus were measured by a control method against Hela cells. In addition, the so-called control method is to inject a virus-containing liquid into the cell in a way that confirms that the cell is infected with the virus. It can investigate the activity of the virus, that is, the frequency of virus infection or the ability of the virus to multiply in the cell. method. The ion concentration is from one side of the cylindrical wind tunnel 31 provided with the ion generating element 12 as described above, and a fan (not shown) is used to make the wind flow at a wind speed of 4m / sec, and the ion is at a distance from the other side. An air ion counter (product number 83-1001B) manufactured by DAN Scientific was set at 10 cm of the generating element, and the ion concentration in the space was measured. The space ambient temperature is 25t and the relative humidity is 60% RH. The ion generation system was confirmed to have a temperature of 0 ° C and a relative humidity of 10% to a temperature of 40 ° C and a relative humidity of 90%. In addition, the above-mentioned blower is used to confirm the ion concentration. In the actual evaluation of microbial removal, the blower is not used, and the wind generated by the spray of the sprayer 11 is set inside the circular wind tunnel 31. As shown in Figure 11, when the frequency of cellular infection of influenza virus when the ion generating element is not operating is 100%, when 50,000, 100,000, and 200,000 (pieces / cm3) ions occur, the cell infection is greatly reduced. Frequency to 3.8%, 2.6%, and 0.5%. It was confirmed that the increase in ion concentration can improve the performance of removing epidemic -37 · (32) 1235671 influenza virus. In addition, as shown in FIG. 12, when the frequency of Sachs virus cell infection is 100% when the ion generating element is not operated, when 50,000 and 10.2 million (pieces / cm3) ions occur, the frequency of cell infection is greatly reduced to '2.6. % And 1.1%. It was confirmed that the increase in ion concentration can improve the performance of removing parvovirus. In addition, as shown in FIG. 13, when the frequency of cell infection of poliovirus is 100% when the ion generating element is not operated, when 50,000 and 200,000 (pieces / cm3) ions are generated, the frequency of cell infection is greatly reduced by 1.0. %, 0.5% and 0.4%, it was confirmed that the performance of poliovirus can be improved due to the increase in ion concentration. The composition of the generated ions is shown in Figure 14. The positive ions are discharged by isoelectric discharge to ionize water molecules in the air to generate hydrogen ions Η +. The water molecules and hydrogen ions in the air are chstering due to the dissolved energy. It is a plasma discharge that ionizes oxygen molecules in the air to produce oxygen ions. Plant 0, which causes water and oxygen ions in the air to cluster (clustering) due to the solvent and energy. The positive and negative ions sent out of the space surround the virus floating in the air, and the toxic surface ’is caused by the chemical reaction of the positive and negative ions to generate an active source of peroxy Η 2 02 or free radicals · OH, which destroys the protein and kills it. This method effectively sterilizes and removes viruses in the air. As a method for investigating the activity of the virus, erythrocyte coagulation can be used. The erythrocyte agglutination reaction is a method of injecting a solution containing a virus into a blood-containing solution and observing the blood's agglutination. Use gram of virus in the presence of virus and 3.3% of gram of sand to remove ionic media and 100,000. Or water molecules can cause chickens to collect pathogens. -38 · (33) 1235671 The red blood cell agglutinin on the surface of the virus acts on most red blood cells, causing the red blood cells to agglutinate and confirm the existence of the virus. In addition, the method of investigating the virus concentration is to dilute the virus to various concentrations with an aqueous solution to confirm whether a erythrocyte agglutination reaction occurs. In contrast, it is also possible to investigate the active virus concentration, that is, the virus concentration having the infectivity of active erythrocyte agglutinin. [Example 7] Fig. 16 is a schematic diagram of a device for evaluating the removal of planktonic pathogenic bacteria. Figure 17 is a graph showing the change over time of the concentration of Staphylococcus in the air at an ion concentration of 200,000 / cm3. The ion generator was the same as that used in Example 6. Fig. 18 is a graph showing the change over time of the concentration of staphylococcus in the air in the ion generating element and the ultraviolet ozone generating device. The space environment temperature is 25 ° C and the relative humidity is 60% PH. The ion generation system was confirmed to have a temperature of 0 ° C, a relative humidity of 10%, a temperature of 40 ° C, and a relative humidity of 90%. Lu In order to detect the removal effect of staphylococcus aureus existing in a certain space by the ion generating element, this experiment uses the same basic structure as shown in FIG. 1. That is, the container 8 is an acrylic plate made of acrylic resin at the rain end of a 1 m X 1 m X 1 m FRP container using a space of 1 m3. Inside this container, ions are installed on the air blowing outlet above the blower with an air volume of 2m3 / min.

Generating element 1. In addition, in order to float the sprayed bacteria for a long period of time, four 15 cm square axial-flow fans (axi a) -flow fans 4 are installed at the four corners of the container 8 with the wind direction (34) 1235671. One end of the acrylic plate portion of the container 8 was provided with an injection tube 5 for spraying bacterial liquid, and this was used as a test device. The test strains were inoculated and preserved in trypticase soy agar medium (BBL ′ Trypticase soy agar) and cultured at 35 ° C for 24 hours. This bacterium was diluted with sterilized physiological saline solution, adjusted and washed, and then used as a test bacterium. 10 ml of the test bacterial solution was put into a glass sprayer and connected to one end of the test device. The other end of the container 18 is connected to a glass dust tester containing 100 ml of a sterilized physiological saline solution. The discharge pressure of the compressed air from the gas sampler in the sprayer was adjusted to 0.48 hPa with the gauge pressure, and the test bacteria were sprayed from the spray port. The spray amount was 1.0 μm (the spray flow rate was 0.1 ml / min X the spray time was 10 min). While spraying the bacterial solution, the axial fan 4 was operated until the end of the test, and continuous operation was performed. When the spraying is finished, the air in the container 8 is sucked and collected for 10 minutes by a suction flow rate of 10 L per minute with a dust measuring device. Take this as 0 minutes. When the ion generating element 1 is operated, the ion generating element and the fan are operated simultaneously. After the start of the operation, after a certain period of time, 100 L of air in the trough trap was sucked in the same way as 0 minutes. The ion generation concentration is 200,000 / cm3. In addition, when the ion generating element 1 is not operated (natural decay), the ion generating element is not operated, and only the fan 4 is operated, and each time it is attracted to capture Collect the air inside the container. In addition, in order to perform a comparison experiment with ozone, a UV-type ozone generator (OZ51N-1, SEN Special Light Source Co., Ltd.) is used, and the ozone generation amount is the same as the ozone generation amount of the ion generating element -40- (35) 1235671 1.63 7mg / h (22t:, 17% RH) for testing. The sterilized physiological salt solution of the air trapped in the container attracted by the dust tester was used as a test solution, and the sterilized physiological salt solution was used to dilute it step by step. 'Incubate at 3 5 ° C for 4 8 hours. After incubation, the number of colonies on the medium was counted, indicating the number of bacteria that attracted air. As shown in Fig. 17, compared with the case where the ion generating element was not operated, it was confirmed that the concentration of phytoplankton had been reduced to about 1 / 10th after 30 minutes. Furthermore, no phytoplankton was detected after 60 minutes. As shown in Fig. 18, after comparing the ion generating element with the ultraviolet type ozone generating device, it was confirmed that the concentration of plankton was reduced to about 1/10 after 60 minutes. Accordingly, the bactericidal effect of Staphylococcus, a representative bacterium of nosocomial infection, was confirmed in accordance with the effect described in Example 6. [Example 8] Fig. 19 is a graph showing the change over time of the concentration of Bacillus in the air at an ion concentration of 200,000 / cm3. The same ion generating apparatus was used as in Example 6. Fig. 20 is a graph showing the change over time of the concentration of planktonic bacillus in the air of an ion generating element and an ultraviolet-type ozone generating device. In order to test the removal effect of the ion-generating element on the planktonic spores existing in a certain space, an acrylic plate was installed at both ends of a 1 m X lm X lm FRp container in a space of 1 m 3. . Inside this container, the air blowing outlet -41-(36) 1235671 above the air blower with an air volume of 8m3 / min is equipped with an ion generating element. In addition, in order to float the sprayed bacteria for a long period of time, four 15 cm square axial fans 4 are installed at the four corners of the container, with the wind direction upward. One end of the acrylic plate portion of the container 8 was provided with an injection tube 15 for spraying bacterial liquid, and this was used as a test device. The test bacterial strains were inoculated with a medium for basal spore formation (Japanese Antibiotics Standard, June 30, 1987, Ministry of Health and Welfare Notice No. 117), and cultured at 35 ° C for 7 days. After the bacteria were washed with a sterilized physiological saline solution, heat treatment was performed at 65 ° C 'for 30 minutes, and spore formation was confirmed under a microscope. The latter was washed with a sterile physiological saline solution and diluted with the latter, and used as spores. The test bacteria solution of 10m] was put into a glass sprayer and connected to one end of the test device. The other end is connected to a glass dust tester containing 100 ml of a sterile physiological saline solution. The discharge pressure of the compressed air from the gas sampler in the sprayer was adjusted to 0.48hPa with the gauge pressure, and the test bacteria were sprayed by spraying. The spray amount is 1.0 π) 1 (spray flow rate of 0.1m) / minx spray time 10m. While spraying the bacterial solution, the axial flow fan 4 was operated until the end of the test, and the continuous operation was performed. At the end of spraying ', the air in the container is sucked and collected for 10 minutes by a suction flow of 10 L per minute with a dust measuring device. This is 0 minutes. When the ion generating element 1 is operated, the ion generating element and the fan are operated simultaneously. After the start of the operation, after a certain period of time, 100 L of air in the trap container was sucked in the same manner as 0 minutes. The ion concentration was 200,000 / cm3. In addition, when the ion generating element 1 is not operated (natural decay) 42 42 · (37) 1235671 The ion generating element is not operated, and only the fan 4 is operated, and the air in the trap container is attracted every time. . The space environment temperature is 25 t and the relative humidity is 60% PH. The ion generation system was confirmed to have a temperature of 0 ° C, a relative humidity of 10%, and a temperature of 40 ° C, and a relative humidity of 90%. In order to perform a comparison experiment with ozone, a UV-type ozone generator (OZ5 1N-1, SEN Special Light Source Co., Ltd.) was used to generate an ozone generation amount of 1.637 mg / h (22 °) equal to the ozone generation amount of the ion generating element. C, 17% RH). The sterilized physiological saline solution in the air trapped in the container attracted by the dust measuring device is a test solution, and the sterilized physiological saline solution is used for stage dilution, and the original solution and each diluted solution are smeared on tryptic casein soybean agar medium (BBL). Incubate at 35 ° C for 48 hours. After incubation, the number of colonies on the medium was counted, indicating the number of bacteria that attracted air. As shown in Fig. 19, it was confirmed that the concentration of phytoplankton was reduced to about 1/10 of that after 30 minutes, as compared with when the ion generating element was not operated. Further, after 120 minutes elapsed ', no phytoplankton was detected. As shown in Fig. 20, when the ion generating element was compared with the ultraviolet type ozone generating device, it was confirmed that the concentration of plankton was reduced by about half after 60 minutes. Accordingly, the bactericidal effect of Bacillus spp. Which formed heat-resistant spores was confirmed by the action described in Example 6. Anthrax and Bacillus are the same genus, and their effects can be expected. [Embodiment 9] -43-(38) 1235671 Fig. 21 is a cross-sectional view showing an air-conditioning apparatus disposed in an air path of an ion generating element blow-out port. Fig. 22 is a graph showing the frequency of cellular infection of plankton virus in the air after 60 minutes with respect to the ion ejection amount in a space of 27L volume. Fig. 23 is a graph showing changes over time in the frequency of virus cell infection in the air when 540,000 positive / negative ions are supplied in a space of 30 m3 in capacity per minute. In the test shown in Figure 22, in order to detect the effect of ion ejection on the frequency of cell infection of plankton influenza virus in the space, the 27L space used in this test is 30cmx30cmx 30cm of chlorinated olefin. A virus spray device and a recovery device are installed at both ends of the container. In this container, an ion generating element is installed in the air blowing port above the blower. In addition, in order to achieve the purpose of floating the sprayed virus for a long time, the axial direction of the axial flow fan is set upward. Influenza virus A (HI NI) A / PR8 / 34: ATCC VR-95 is inoculated into the allantoic membrane cavity of developing eggs, cultured in an incubator, and allantoic fluid is taken as the test virus fluid . Put the test virus solution of ΙΟηαΙ into a glass sprayer and connect it to one end of the test device. The other end is connected to a glass dust tester containing 100 m 1 of sterile physiological salt solution. The sprayer comes from a gas sampler The discharge pressure of the compressed air was adjusted to 0.48hPa with the gauge pressure, and the test bacteria were sprayed from the spray port. The spray volume was 3.0ml (spray flow rate 0.1ml / minx spray time 30min). While spraying the virus solution, the axial flow Fan 4 is operated until the end of the test, and continuous operation is performed. At the end of the spray, the air in the container is dust-measured by a dust detector at a rate of 44-39 (39) 1235671 for 10 minutes per minute to attract and capture for 30 minutes. This is 0 minutes. 1 hour after the start of the operation, 100L of air in the trap container is sucked in the same manner as 0 minutes. The suction bow of the dust tester | The PBS (-) of the air in the capture test device is the test solution, and it is popular Cold virus Measured by the control method of MDCK cells. The input voltage of the ion generating element was adjusted to adjust the ion ejection amount. When the positive and negative ion ejection amounts were 0 / cm3 · min and the cell infection frequency was 100%, it was confirmed that 270,000 / cm3 · The frequency of cell infections with an ion ejection amount of above minus rapidly decreased, and it was confirmed that the positive and negative ion ejection amounts were 270,000 / cm3 or more, respectively, which had the effect of reducing virus infection. Furthermore, in order to confirm the actual use in living environment space The effect is shown in the test shown in FIG. 23. The air conditioning device 60 shown in FIG. 21 is installed in a space volume of 30 m3, and the dust collecting filter 64b and the deodorizing filter 64a are removed, so that the ion generating element 65 Residual rate of plankton virus in the space during operation. Here, when the structure of the air-conditioning apparatus 60 shown in FIG. 21 is described, the air-conditioning apparatus 60 is formed with an air intake port 62 ′ device 61 in front of the indoor device 61. An air outlet 63 is formed above the air inlet 62. A deodorizing filter 64a and a dust collection filter 6 "are provided in the air inlet 62, and an ion generator is provided near the air outlet 63. The ion generating device 67 formed by the element 65 and the high-voltage power source 66. Second, the air fan 68 inside the device exhausts the air sucked in by the air suction port 62 through the air blowing port 63 to the outside. At this time, the ion generating device 67 When driven, it releases ionized air -45- (40) 1235671 The air conditioning device configured as above, is equipped with an ion generating element 65 on the blowing air path, and the air sucked in by the suction port 62 is discharged through the blowout port 63. The air contains ions and can release ions into the space. Therefore, ions can be added not only to the inhaled air but also to the entire space. The virus concentration measurement was performed in the same manner as in the test performed in FIG. 22. 5.4 million ions / cm3 of positive and negative ions were supplied in one minute. Understand that the frequency of cellular infections per hour is reduced to 1/10. In this way, regarding the volume of an air-conditioning apparatus actually used in a living environment, it is understood that the effect of virus inactivation in the air can be evaluated. The positive and negative ions sent out of the space surround the virus floating in the air. On the surface of the virus, the active source of hydrogen peroxide H202 or free radical · OH is generated by the chemical reaction of the positive and negative ions, which destroys the protein and kills it. According to this method, viruses in the air can be effectively sterilized. In addition, in the present embodiment, the ion system indicates both positive and negative ions. Regarding the ion concentration, each ion concentration is approximately equal, and the average radon is recorded. In addition, in all the above-mentioned embodiments, as the particle release method, the Lenar effect is used, and even if the liquid is ejected or vibrated, physical separation is used to generate the particles with electric charges. The effect of the present invention is obtained. In addition, as the particles, positive ions, negative ions, a mixed gas of positive and negative ions, charged particles such as α-rays and / 3-rays other than the above, or particles of various ionized gas particles, radicals, and pharmaceutical particles, etc. The same effects as the present invention are obtained. -46- (41) 1235671 The effect of the invention is as explained above. According to the present invention, a microorganism is allowed to float in a certain space. After the microorganism is irradiated with ions, the germicidal treatment of the microorganism / particles used, then the microorganism is taken. The measurement is carried out to achieve an evaluation test-to determine the effectiveness of the above-mentioned particles on the sterilization treatment ability of microorganisms. &lt; Brief description of the drawings &gt; Fig. 1 is a schematic configuration diagram showing a first embodiment of the apparatus for evaluating microorganism removal according to the present invention. Fig. 2 is a schematic configuration diagram showing a second embodiment of the apparatus for evaluating microorganism removal according to the present invention. &lt; Fig. 3 shows the measurement results of Example 1, and the measurement results of the microorganisms used in the sterilization treatment when the ion concentration was changed. Fig. 4 shows the measurement results of Example 2 regarding the results of measurement of microorganisms taken with and without ions being sent. 0 FIG. 5 is a photograph obtained by photographing the microorganisms taken in Example 2. FIG. Fig. 5 (a) is a photograph of the microorganisms taken when the ion is sent out, and Fig. 5 (b) is a photograph of the microorganisms taken when the ion is not taken out. Fig. 6 shows the measurement results of Example 3, the measurement results of the microorganisms taken in the stirred container and when not stirred. Fig. 7 shows the measurement results of Example 4 regarding the measurement results of the microorganisms taken when the ions were sent and when the ions were not sent. -47-(42)! 235671 Fig. 8 is a schematic diagram showing the third embodiment and the sixth embodiment of the apparatus for evaluating the removal of microorganisms. Fig. 9 is a schematic configuration diagram showing a fourth embodiment of the apparatus for evaluating the removal of planktonic microorganisms. FIG. 10 is a schematic configuration diagram showing a fifth embodiment of the apparatus for evaluating the removal of plankton virus. Fig. 11 is a graph showing the frequency of cellular infection of influenza virus according to the ion concentration of Example 6. FIG. 12 is a graph showing the frequency of cell infection of the Quexach virus according to the ion concentration of Example 6. FIG. Fig. 13 is a graph showing the frequency of cell infection of polio virus according to the ion concentration of Example 6. FIG. 14 is a mass spectrum chart showing positive ions and negative ions generated by the ion generating element of Example 6. FIG. FIG. 15 is a flowchart showing an evaluation test of Example 6. FIG. Fig. 16 is a schematic diagram of a device for evaluating the removal of planktonic pathogenic bacteria in Example 7. Fig. 17 is a graph showing the change over time of the concentration of Staphylococcus in the air at an ion concentration of 200,000 particles / cm3 in Example 7. Fig. 18 is a graph showing the change over time of the concentration of staphylococcus in the air in the ion generating element and the ultraviolet-type ozone generating device of Example 7; Fig. 19 is a graph showing the change over time of the concentration of Bacillus sp. (Baci) us in the air at an ion concentration of 200,000 / cm3 in Example 8. • 48-(43) 1235671 Fig. 20 is a graph showing the change over time of the concentration of planktonic bacillus in the air in the ion generating element and the ultraviolet-type ozone generating device of Example 8. Fig. 21 is a cross-sectional view showing an air-conditioning apparatus provided in an air path of an ion generating element blow-out port in Example 9. / FIG. 22 is a graph showing the frequency of cell infection of floating virus in the air according to the amount of ion ejection in Example 9. Fig. 23 is a graph showing changes over time in the frequency of infection of virus cells in the air according to the amount of ion ejection in Example 9. Φ Symbol description 1 Ion generator 2 Ion generator □ 3 Microbial collection tube 3a Microbial collection □ 4 Mixer 5 Microbial injection tube 5a Microbial injection □ 6 Microbial collector 7 ik 丄 8 Container 10 Evaluation device for microorganism removal 11 Microbial sprayer 12 Ion Generating element 12a Ion generating electrode 49 · (44) (44) 1235671 13 Microbial collection tube 13a Microbial collection port 15 Microbial injection tube 15a Microbial injection port 18 Container 20 Device for evaluating microorganism removal 3 1 Wind tunnel 35 Container

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Claims (1)

1235671 ⑴ Pick up, apply for patent scope 1 · A method for evaluating the removal of microorganisms, which is characterized by supplying microorganisms to the internal space of the container and simultaneously irradiating particles formed by positive and negative ions used for sterilizing the microorganisms. After the particles are irradiated, Take microorganisms' for the determination of the taken microorganisms. 2-A method for evaluating the removal of microorganisms, characterized in that a virus as a microorganism is supplied to the inner space of the container, and particles used for sterilizing the microorganism are irradiated. After the particles are irradiated, the microorganisms are taken and the measured microorganisms are measured. . 3. The method of evaluating microorganism removal as described in item 1 or 2 of the scope of patent application, wherein after the particle is irradiated, the measurement of the microorganism is performed, and further, the same conditions as when irradiating the particle to sterilize the microorganism Next, the microorganisms are supplied, and the particles are not irradiated. After the microorganisms naturally decay, the microorganisms are collected and the measured microorganisms are measured. 4 · If the method for assessing the removal of microorganisms is set out in item 1 or 2 of the scope of the patent application, wherein the determination is a determination of the concentration of the microorganism or a determination of the activity. 5 · If the method for evaluating microorganism removal in the first or second scope of the patent application is applied, the measurement of the microorganisms used is to further measure the change over time according to the time of irradiation of the particle. 6 · If the method for assessing the removal of microorganisms is set out in item 1 or 2 of the scope of the patent application, wherein the determination of the microorganisms used is to further determine the concentration dependence of the removal performance of the particles. 7. The method for removing microorganisms in accordance with item 1 or item 2 of the scope of the patent application -51 · (2) (2) 1235671, wherein the supply of microorganisms to the internal space of the container is to make the solution of dispersed microorganisms fog And spray. 8. The method for removing microorganisms as described in item 1 or 2 of the scope of the patent application, wherein the method for evaluating the microorganisms is the use of cell culture of microorganisms, erythrocyte agglutination reaction of microorganisms, or allergic reaction of microorganisms. 9 · The method for evaluating the removal of microorganisms according to item 2 of the scope of the patent application, wherein the particles used to sterilize the microorganisms are particles generated by any of air discharge, radiation in the air, and Lenard effect. . 10 · The method for evaluating the removal of microorganisms according to item 2 of the scope of the patent application, wherein the particles used for sterilizing the microorganisms are any of radiation, X-rays, 7 rays and electromagnetic waves. 1 1 · The method for evaluating the removal of microorganisms according to item 2 of the scope of the patent application ′ wherein the particles for sterilizing the microorganisms are pharmaceutical particles. 1 2 · The method for evaluating the removal of microorganisms according to item 1 of the scope of the patent application, wherein the microorganism is one or a combination of two or more selected from the group consisting of bacteria, fungi, viruses, and allergens. 1 3. If the method for evaluating microorganism removal is described in item 1 or 2 of the scope of the patent application, regarding the space inside the container, when supplying microorganisms, for the microorganisms supplied in the container, the inside of the container is stirred from the lower side. space. 14. An apparatus for evaluating the removal of microorganisms, comprising an internal space capable of supplying microorganisms and a sterilizing treatment for the microorganisms ►52- (3) 1235671 container, and supplying microorganisms to the container, and the container In the internal space, the biological removal means of the particles formed by the negative ions is used for the biological taking measures of the microorganisms, and the measurement is used for the evaluation. 15. A kind of container for evaluating the microorganisms that can be used to supply the bacteria as viruses, and a means for supplying micro-organisms, and a method for removing microorganisms using particles in the container. After the microorganisms are sterilized, take the The microorganisms are taken by means of 16. The device for removing the scope of the patent application, wherein the microorganisms and the means of taking the microorganisms are arranged side by side to the downstream side. 1 7 · The device for removing the scope of the patent application, wherein the microorganisms are interposed between the microorganisms, and the microorganism removing hand is arranged on the inside. 18 · The device for removing the scope of the patent application 5, wherein the microorganisms are arranged in the microorganism supply. The microorganism supply means for the internal space of the device is provided with a means for removing positive microorganisms for sterilizing microorganisms, and a device for removing microorganisms by means of the microorganisms after the micro-sterilization treatment. The device is characterized by having an internal space. And the micro-organism kills the microorganism in the space of the micro-biological part of the container internal space, and supplies the germicidal treatment microorganism and the microorganism-removing microorganism-removing microorganism-receiving method to measure the microorganisms for evaluation. The assessment means for supplying microorganisms in item 14 or item 15 and the means for removing microorganisms have air passages for microorganisms. The wind tunnel in the air passages for assessing supply means for microorganisms in item 14 or 15 above and the means for taking the microorganisms, the wind tunnel. Paragraph. The evaluation microorganisms of item 14 or item 15 and the area other than the vertical direction below the microorganisms' removal method. • 53- (4) 1235671 1 9. The device to be removed as described in item 14 of the scope of patent application, in which other containers are located outside the container. 2 〇 As described in item 14 of the scope of patent application, the device 5 is a stirring means used in the internal space of the container. 21 · The device as claimed in item 14 of the patent application scope or removed, wherein the microorganism-dispersed solution of the microorganisms is supplied in a mist form and sprayed on. 22. If the patent application scope item 14 or removed device, which sterilizes the micro-born air discharge, radiation in the air, the gas generated by the effect) is released and constitutes 23. If the patent application scope item 15 is set, Among them, the microorganisms are sterilized by light, r-rays, and electromagnetic waves. 24. For example, the scope of application for patent No. 15 is set, wherein the microorganisms removal means is composed of pharmaceutical particles of irradiation particles. 5 The evaluation microorganism of item 15 is provided with an evaluation microorganism space which can cover the container according to item 15 of the container, and the microorganism supply means for stirring the inside of the evaluation microorganism of item 15 is to make the internal space of the container constitute: The particle used for the evaluation of microorganisms in item 15 is composed of either one and the Lenar effect (the evaluation of removal of microorganisms by Lenard 〇, the radiation of any one, the evaluation of removal of microorganisms by X 〇 is used to kill the microorganism-54. -