JP2011139682A - Device and method for measuring inactivation effect of floating-infective influenza virus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a device for inactivating floating-infective influenza virus, or to provide a method for quantitatively measuring the inactivation effect of a liquid agent or inactivating filter. <P>SOLUTION: The method comprises the following process: a chamber is injected with a defined quantity of floating-infective influenza virus; a defined volume of air is sampled from the chamber at defined time intervals by repeatedly conducting a defined number of cycles of the step 102 of introducing a defined volume of air into the chamber and the step 103 of sampling a defined volume of air exhausted from the chamber; such a treatment as to pass the defined volume of air thus sampled through a filter enabling the floating-infective influenza virus to be caught is conducted for each of the defined volume of air sampled at the defined time intervals; and by conducting the quantitative analysis of the infection titer of the floating-infective influenza virus caught in each of the filters; and the change with time of the infective titer of the floating-infective influenza virus in the case of the influenza-inactivating device's being installed in the chamber is measured. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

The present invention relates to an apparatus and method for quantitatively measuring the effect of inactivating a floating infectious influenza virus floating in the atmosphere (killing the proliferation function as a virus).

  In recent years, in response to outbreaks of swine-derived new influenza (pandemic H1N1) and highly pathogenic avian influenza (H5N1), as countermeasures against these influenza infections, masks, gargles, hand washing recommendations, specific vaccines, and therapeutic drugs Are being stockpiled and developed.

  These airborne infectious influenza viruses are concerned not only with normal droplet infection but also with air infection in a space with limited ventilation. For example, air infection has been reported from 1 out of 54 passengers in an air-conditioned plane to 39 passengers (Am J Epidemiol, 110: 1-6, 1979). Particularly in the influenza epidemic period, it is important for medical institutions such as examination rooms and hospital rooms to take measures against airborne influenza and airborne infection. In addition to medical institutions, it is important to take measures against airborne influenza and airborne infections in public spaces such as buildings and transportation facilities, and even in homes.

  Therefore, infectious influenza that floats in the environment that causes such droplets and air infections can be inactivated widely (used in this specification to kill the proliferation function as a virus) at home. If an apparatus and a method that can be developed can be developed, the invisible risk of droplets and air infection can be reduced, and it can be an extremely effective means for countermeasures against epidemic influenza.

  Therefore, in recent years, an apparatus that can be easily installed not only for business use but also for home use, which inactivates airborne infectious influenza virus, is being developed.

  The present applicant has already filed a utility model for a mechanism for improving the maintainability for home use, which is a device for decomposing and removing pollutants in the air using a photocatalyst, and has registered a utility model. (Patent Document 1 below).

  In addition, as a method for removing microorganisms including viruses, there is a method for irradiating microorganisms with particles such as ionized ions to sterilize them. Patent Document 2 listed below discloses an invention for a microorganism removal evaluation apparatus that measures and evaluates the ability to sterilize and remove microorganisms by this sterilization method.

  In this patent document 2, microorganisms are irradiated with particles such as ionized ions in a container, and then a predetermined volume of air is sucked from the container by an air sampler to lead to a sterilized physiological saline solution. A method of recovery is described.

Utility Model Registration No. 3150894 (No. 2009-1595) JP 2004-159508 A

  The present applicant has advanced an air purifier described in the above-mentioned Patent Document 1 based on an air purifier suitable for home use, and an air purifier with improved performance for decomposing and removing airborne infectious influenza virus in the air. It is being developed.

  However, a measurement device that can quantitatively measure the inactivation effect of a device that inactivates airborne infectious influenza virus (referred to as an inactivation device in this specification), including such an air purifier, It did not exist before.

  The microorganism removal evaluation apparatus described in Patent Document 2 is intended for a method of sterilizing a microorganism by irradiating particles such as ionized ions and the like. As described in Patent Document 1, floating infectivity is caused by a photocatalyst. It does not cover the method of oxidative degradation of influenza virus.

  Further, in Patent Document 2, since a predetermined volume of air is sucked per unit time by an air sampler, in order to collect the same constant volume of air at regular time intervals, the air sampler must be stopped each time. However, there is a problem that the operation is troublesome even if the apparatus is large. In addition, the container and the apparatus for sucking and collecting cannot be separated. For this reason, after air is guided to the sterilized physiological saline and the microorganisms are collected, it is not possible to move to the next cycle until the microorganism is replaced with a new sterilized physiological saline.

  The present invention has been made in view of such circumstances, so that the inactivation effect of the inactivation device or solution or the inactivation filter itself for inactivating the infectious influenza virus can be quantitatively measured by a simple method. It is a problem to be solved.

The first invention is
When an inactivation device that inactivates airborne infectious influenza virus is installed in the chamber, the method for measuring the inactivation effect of airborne infectious influenza virus by the inactivation device,
Inject a predetermined amount of airborne infectious influenza virus into the chamber,
An air introduction step for introducing a predetermined volume of air into the chamber;
A predetermined time interval is obtained by repeatedly performing each step consisting of an air sampling step for sampling a predetermined volume of air exhausted from the chamber in response to the predetermined volume of air being introduced into the chamber. To collect a predetermined volume of air from inside the chamber,
A process of passing the collected predetermined volume of air through a filter capable of capturing airborne infectious influenza virus is performed for each of the predetermined volume of air collected at predetermined time intervals,
Quantitative analysis of the infectious titer of airborne infectious influenza virus trapped in each filter to measure the time course of the infectious titer of airborne infectious influenza virus when the inactivation device is installed in the chamber It is characterized by that.

The second invention is the first invention,
By performing quantitative analysis of the infectious titer of airborne infectious influenza virus trapped in each filter for the case where the inactivation device is installed in the chamber and the case where the inactivation device is not installed, the inactivation device is placed in the chamber. Measuring the time course of the infectious titer of airborne infectious influenza virus when installed in the chamber and the time course of the infectious titer of airborne infectious influenza virus when the inactivation device is not installed in the chamber Features.

The third invention is the first invention or the second invention,
The inactivation device is an air cleaner that takes in outside air and discharges air that has passed through a virus inactivation filter including a photocatalytic filter to the outside.

4th invention is 1st invention or 2nd invention or 3rd invention,
Furthermore, we perform quantitative analysis of the number of floating infectious influenza virus particles trapped by the filter, and evaluate the performance of the inactivation device by combining the quantitative analysis result of the virus particle number with the quantitative analysis result of the infectious titer. It is characterized by doing.

A fifth invention is the first invention,
The inactivation device is a device that generates positive ions and negative ions by causing an ionization phenomenon in the atmosphere.

A sixth invention is the first invention,
An organism capable of being infected with airborne infectious influenza virus is allowed to remain in the chamber, and after a predetermined time has elapsed since the airborne infectious influenza virus was injected into the chamber, the organism was recovered from the chamber, and the organism was recovered. It is characterized by measuring the infectivity reduction effect of the floating infectious influenza virus when the inactivation device is installed.

The seventh invention
When an inactivating agent that inactivates airborne infectious influenza virus is sprayed in the chamber, the method for measuring the inactivating effect of airborne infectious influenza virus by the inactivating agent,
Inject a predetermined amount of airborne infectious influenza virus into the chamber,
An air introduction step for introducing a predetermined volume of air into the chamber;
A predetermined time interval is obtained by repeatedly performing each step consisting of an air sampling step for sampling a predetermined volume of air exhausted from the chamber in response to the predetermined volume of air being introduced into the chamber. To collect a predetermined volume of air from inside the chamber,
A process of passing the collected predetermined volume of air through a filter capable of capturing airborne infectious influenza virus is performed for each of the predetermined volume of air collected at predetermined time intervals,
Quantitative analysis of the infectious titer of airborne infectious influenza virus trapped in each filter to measure the time course of the infectious titer of airborne infectious influenza virus when the inactivating agent is sprayed into the chamber It is characterized by that.

The eighth invention
When an inactivation device that inactivates airborne infectious influenza virus is installed in the chamber, the device for measuring the inactivation effect of airborne infectious influenza virus by the inactivation device,
A virus inlet provided in the chamber for injecting a predetermined amount of floating infectious influenza virus into the chamber;
An air inlet for introducing air into the chamber;
A predetermined volume of air introduction bag into which air is pushed into the chamber through the air introduction port;
An air exhaust port through which air is exhausted from the chamber as the predetermined volume of air is pushed into the chamber through the air inlet and introduced into the chamber;
An air sampling bag for collecting air exhausted through the air exhaust port;
A suction means for sucking a predetermined volume of air collected in the air collection bag through the suction passage;
A filter provided on the suction passage and capable of capturing a floating infectious influenza virus;
Collect a predetermined volume of air with a bag for collecting air at a predetermined time interval,
The collected predetermined volume of air is sucked by the suction means,
Quantitative analysis of the infectious titer of airborne infectious influenza virus trapped in each filter to measure the time course of the infectious titer of airborne infectious influenza virus when the inactivation device is installed in the chamber It is characterized by that.

The ninth invention
When an inactivating agent that inactivates airborne infectious influenza virus is sprayed in the chamber, the device for measuring the inactivating effect of airborne infectious influenza virus by the inactive agent,
A virus inlet provided in the chamber for injecting a predetermined amount of floating infectious influenza virus into the chamber;
An air inlet for introducing air into the chamber;
A predetermined volume of air introduction bag into which air is pushed into the chamber through the air introduction port;
An air exhaust port through which air is exhausted from the chamber as the predetermined volume of air is pushed into the chamber through the air inlet and introduced into the chamber;
An air sampling bag for collecting air exhausted through the air exhaust port;
A suction means for sucking a predetermined volume of air collected in the air collection bag through the suction passage;
A filter provided on the suction passage and capable of capturing a floating infectious influenza virus;
Collect a predetermined volume of air with a bag for collecting air at a predetermined time interval,
The collected predetermined volume of air is sucked by the suction means,
Quantitative analysis of the infectious titer of airborne infectious influenza virus trapped in each filter to measure the time course of the infectious titer of airborne infectious influenza virus when the inactivating agent is sprayed into the chamber It is characterized by that.

The tenth invention is
A method of measuring the inactivation effect of airborne infectious influenza virus by an inactivation filter that captures and inactivates airborne infectious influenza virus in introduced air or exhaust air,
Air containing airborne infectious influenza virus is inhaled from one side of the inactivation filter, exhausted from the other side of the inactivation filter,
Collect a predetermined volume of air exhausted from the other surface of the inactivation filter,
The collected volume of air is passed through a capture filter that can capture airborne influenza viruses,
The inactivation filter performance is evaluated by performing a quantitative analysis of the infectious titer or / and the number of virus particles of the suspended infectious influenza virus captured by the capture filter.

The eleventh invention is
A method of measuring the inactivation effect of airborne infectious influenza virus by an inactivation filter that captures and inactivates airborne infectious influenza virus in introduced air or exhaust air,
Air containing airborne infectious influenza virus is inhaled from one side of the inactivation filter, exhausted from the other side of the inactivation filter,
Introducing the air exhausted from the other surface of the inactivation filter into a chamber in which a living organism capable of infecting airborne infectious influenza virus is located;
After a predetermined time has elapsed since air containing airborne infectious influenza virus was introduced into the chamber, the life form was collected from the chamber, and the life form was inspected. It is characterized by measuring the infectivity reduction effect.

In a twelfth aspect based on the eleventh aspect,
Exhaust the air in the chamber from the outlet of the chamber;
The air exhausted from the chamber is passed through a capture filter capable of capturing airborne infectious influenza virus;
The inactivation filter performance is evaluated by performing a quantitative analysis of the infectious titer or / and the number of virus particles of the suspended infectious influenza virus captured by the capture filter.

The thirteenth invention
In a device that measures the inactivation effect of airborne infectious influenza virus by an inactivation filter that captures and inactivates airborne infectious influenza virus in the introduction air or exhaust air,
Air generating means for generating air containing airborne infectious influenza virus;
A suction passage communicating the air generating means and one surface of the inactivation filter;
A collection bag of a predetermined capacity provided on the other surface side of the inactivation filter, for collecting air exhausted from the other surface of the inactivation filter;
An exhaust passage communicating the other surface of the inactivation filter and the collection bag;
Inhalation / exhaust means for inhaling air containing airborne infectious influenza virus from one surface of the inactivation filter through the inhalation passage and exhausting from the other surface of the inactivation filter through the exhaust passage. ,
An air sampling bag for collecting air exhausted through the exhaust passage;
A suction means for sucking a predetermined volume of air collected in the air collection bag through the suction passage;
Provided on the suction passage, and provided with a capture filter capable of capturing floating infectious influenza virus,
The inactivation filter performance is evaluated by performing a quantitative analysis of the infectious titer or / and the number of virus particles of the suspended infectious influenza virus captured by the capture filter.

The fourteenth invention is
In a device that measures the inactivation effect of airborne infectious influenza virus by an inactivation filter that captures and inactivates airborne infectious influenza virus in the introduction air or exhaust air,
Air generating means for generating air containing airborne infectious influenza virus;
A suction passage communicating the air generating means and one surface of the inactivation filter;
A chamber that is provided on the other surface side of the inactivation filter and into which air exhausted from the other surface of the inactivation filter is introduced, in which a living organism that can be infected with airborne infectious influenza virus is located A chamber;
An exhaust passage communicating the other surface of the inactivation filter and the chamber;
Inhalation / exhaust means for inhaling air containing airborne infectious influenza virus from one surface of the inactivation filter through the inhalation passage and exhausting from the other surface of the inactivation filter through the exhaust passage. Is provided,
After a predetermined time has elapsed since air containing airborne infectious influenza virus was introduced into the chamber, the life form was collected from the chamber, and the life form was inspected. It is characterized by measuring the infectivity reduction effect.

In a fifteenth aspect based on the fourteenth aspect,
An exhaust port provided in the chamber for exhausting air in the chamber;
An air sampling bag for collecting air exhausted through the exhaust port;
A suction means for sucking a predetermined volume of air collected in the air collection bag through the suction passage;
Provided on the suction passage, and provided with a capture filter capable of capturing floating infectious influenza virus,
The inactivation filter performance is evaluated by performing a quantitative analysis of the infectious titer or / and the number of virus particles of the suspended infectious influenza virus captured by the capture filter.

The first to sixth inventions and the eighth invention are intended for inactivation devices that inactivate airborne influenza viruses by installing and operating them in an environment where airborne influenza viruses exist. The present invention relates to a method or apparatus for measuring an effect. According to the first to sixth inventions and the eighth invention, it is possible to measure the change over time of the infectious titer of the floating infectious influenza virus when the inactivation apparatus is installed in the chamber.

In particular, according to the fourth invention, the performance of the inactivation apparatus can be evaluated by associating the quantitative analysis result of the infectious titer with the quantitative analysis result of the number of virus particles.

7th invention and 9th invention are the method or apparatus which measures the inactivation effect for the inactivating agent which inactivates airborne infectious influenza virus by spraying in the environment where airborne infectious influenza virus exists About. According to the seventh and ninth inventions, it is possible to measure the change over time of the infectious titer of the floating infectious influenza virus when the inactivating agent is sprayed into the chamber.

The tenth to fifteenth aspects of the present invention relate to a method or apparatus for measuring the inactivation effect of an inactivation filter that captures and inactivates airborne infectious influenza virus in introduced air or exhaust air. According to the tenth to fifteenth inventions, the performance of the inactivating filter can be evaluated.

FIGS. 1A and 1B are diagrams illustrating an example of the configuration of the measurement apparatus according to the first embodiment. FIG. 2A is a diagram showing a modification of the partial configuration of the measurement apparatus according to the first embodiment. FIGS. 2B and 2C are a part of the measurement apparatus according to the second embodiment. It is the figure which showed the modification of the structure, respectively. FIGS. 3A and 3B are flowcharts showing the procedure of the measurement method of the first embodiment, and FIGS. 3C and 3D are other procedures of the measurement method of the first embodiment. It is the flowchart which showed. FIG. 4 is a diagram illustrating an example of a measurement result according to the first embodiment. FIG. 5 is a diagram illustrating a device configuration example of the measurement device according to the second embodiment.

  Hereinafter, embodiments of an apparatus and a method for measuring the inactivation effect of airborne infectious influenza virus according to the present invention will be described with reference to the drawings.

  The measurement of the present invention is performed at a location that meets the standards for an experimental facility for infectious disease viruses.

(First embodiment)
FIGS. 1A and 1B show an example of the device configuration of the measurement apparatus according to the first embodiment.

As shown in FIG. 1 (a), the chamber 1 is hermetically sealed so as not to leak air except for the openings such as the virus inlet 2, the air inlet 3, and the air outlet 4, and the interior has a predetermined volume. It is the housing which becomes. For example, a cubic box can be used as the chamber 1.

In the chamber 1, an inactivation device 20 which is a measurement target is installed.

The inactivation device 20 to be measured is, for example, an air cleaner that takes in outside air and discharges air that has passed through a virus inactivation filter including a photocatalytic filter to the outside. Moreover, the inactivation apparatus 20 to be measured is an apparatus that generates positive ions and negative ions by causing an ionization phenomenon in the atmosphere, for example.

In the case where the chamber 1 is a casing configured by combining each side, for example, at least one side 1a of the chamber 1 functions as the loading / unloading port 1a of the inactivation device 20. At least one side 1a of the chamber 1 is freely detachable from the other side. After the inactivation device 20 is loaded from the loading / unloading port 1a and installed in the chamber 1, the loading / unloading port 1a of the chamber 1 is connected to the surrounding other sides 1b, 1c, 1d, and 1e, and the surrounding air Sealed to prevent leakage. In the case where the inactivation apparatus 20 is an apparatus that operates using an external AC power supply as a drive source, a hole is formed in the chamber 1 through which cords for connecting to the external AC power supply are passed. In this case, the periphery of the hole is sealed so as not to leak air. The chamber 1 may be a casing in which the sides are fixed to each other, and a loading / unloading exit 1a that can be freely opened and closed may be formed in a part thereof.

The chamber 1 is provided with a virus injection port 2 for injecting a predetermined amount of floating infectious influenza virus into the chamber 1. For example, when the floating infectious influenza virus used for measurement is stored in the state of a preservation solution, the preservation solution may be atomized and injected from the virus injection port 2. In this case, for example, the virus liquid can be injected by spraying means such as a spray. Further, for example, an ultrasonic type or jet type atomizing device can be used. For example, a commercially available ultrasonic spray generator can be connected to the virus injection port 2 so that the virus preservation solution is atomized and injected into the chamber 1 from the virus injection port 2. The virus inlet 2 is sealed so that no air leaks through the virus inlet 2 after virus injection.

The chamber 1 is provided with an air inlet 3 through which air is introduced into the chamber 1.

A predetermined volume of air introduction bag 5 for pushing air into the chamber 1 is connected to the air introduction port 3 through the air introduction port 3. As the air introduction bag 5, a bag capable of discharging a predetermined volume of air by being pushed in is used.

In the chamber 1, air of a predetermined volume in the air introduction bag 5 is exhausted from the chamber 1 as it is pushed into the chamber 1 through the air inlet 3 and introduced into the chamber 1. An exhaust port 4 is provided.

The air exhaust port 4 is connected to an air sampling bag 6 for collecting the air exhausted through the air exhaust port 4 through the sampling port 6a. The collection bag 6 is removed from the air exhaust port 4 after the air in the chamber 1 is collected.

Air is pushed into the shrunk air introduction bag 5 through the air exhaust port 4 and the chamber 1, and air is newly inflated.

The air exhaust port 4 is sealed so as not to cause air leakage through the air exhaust port 4 after the collection bag 6 from which the air in the chamber 1 has been collected is removed from the chamber 1.

As shown in the sectional view of FIG. 2A, it is possible to implement the air introduction bag 5 with a bellows structure and the air sampling bag 6 with a bellows structure. In this case, the air introduction bag 5 is provided with, for example, a one-way outside air intake valve 5 a that does not open when pushed into the chamber 1, and the chamber 1 has a one-way air discharge valve that opens only when pushed into the chamber 1. 1 g is provided. The air exhaust port 4 on the air sampling bag 6 side is provided with a one-way air introduction valve 1 h that opens only when air is introduced from the air introduction bag 5 into the chamber 1.

According to this configuration, fresh air outside the chamber 1 can enter the air introduction bag 5 without causing contaminated air in the chamber 1 to enter.

Further, after the air introduction bag 5 is pushed in and contracted, the air introduction bag 5 is removed from the air introduction port 3, and the air introduction bag 5 is newly filled with a compressor or the like, and again, the air introduction port 3. It is also possible to mount and connect to.

Further, it is possible to directly connect a discharge port of an air introduction means such as a compressor to the air introduction port 3 so as to send a predetermined volume of air into the chamber 1.

In addition, a manual open / close valve and shutter are provided in the air exhaust port 4 of the chamber 1, and when the air is discharged to the air introduction bag 5, the air exhaust port 4 is opened, and the air sampling bag 6 is removed from the chamber 1. After that, the air exhaust port 4 can be closed and sealed.

On the other hand, FIG. 1 (b) shows a state where the air sampling bag 6 shown in FIG. 1 (a) is removed from the chamber 1 and attached to the suction device 11. The suction device 11 includes a suction passage 7 and suction means 8.

A suction passage 7 is connected to the collection port 6 a of the air collection bag 6.

A suction means 8 is connected to the suction passage 7 to suck a predetermined volume of air collected in the air collection bag 6 through the suction passage 7. For example, a water flow pump is used as the suction means 8, and the suction port of the water flow pump is connected to the suction passage 7.

A filter case 9 is provided on the suction passage 7. The filter case 9 is configured to be freely detached from the suction passage 7 and attached to the suction passage 7.

The filter case 9 houses a capture filter 10 that can capture airborne infectious influenza virus in the air sucked through the suction passage 7.

FIGS. 3A and 3B show a procedure of the measurement method in a flowchart.

(Virus injection step)
First, a predetermined amount of floating infectious influenza virus is injected into the chamber 1 from the virus injection port 2 (step 101).

(Air introduction step)
Next, a predetermined volume of air is introduced into the chamber 1 from the air inlet 3 (step 102).

(Air sampling step)
As a predetermined volume of air is introduced into the chamber 1, a predetermined volume of air exhausted from the air exhaust port 4 of the chamber 1 is collected by the air collection bag 6 (step 103).

Steps 102 and 103 of the above (air introduction step) and (air sampling step) are repeated a predetermined number of cycles. Thus, a predetermined volume of air is collected in the air collection bag 6 from the chamber 1 at predetermined time intervals. In this case, the time intervals for collecting air may be equal intervals or unequal intervals.

In this case, the air collection bag 6 may be reused after the collected air is sucked.

(Removal of air sampling bag 6)
When a predetermined volume of air is collected in the air collection bag 6, it is removed from the chamber 1 (step 104).

(Capture virus in the filter for capture)
A suction device 11 shown in FIG. 1B is attached to the air sampling bag 6 removed from the chamber 1 to suck air. As a result, the suction air passes through the capture filter 10, and the floating infectious influenza virus in the air collection bag 6 is captured by the capture filter 10. The trapping filter 10 is collected from the filter case 9 each time the air in one air sampling bag 6 is sucked and passed through the filter 10. A new capturing filter 10 is accommodated in the filter case 9. (Step 105).

(Installation of air sampling bag 6)
The air collection bag 6 from which the air has been sucked is attached to the chamber 1 again (step 106). Thereafter, the procedure returns to step 102 and the same process is repeated a predetermined number of cycles.

In this way, the floating infectious influenza virus contained in each of the predetermined volumes of air collected at predetermined time intervals is captured by each capture filter 10.

(Quantitative analysis)
In parallel with the processing of steps 101 to 106 described above, or after the completion of the processing of all cycles of steps 101 to 106 described above, the floating infectious influenza virus is collected from the capture filter 10. Then, using the collected floating infectious influenza virus as a sample, quantitative analysis of the infectious titer is performed. By performing this quantitative analysis on each capture filter 10, the change over time of the infectious titer of the floating infectious influenza virus when the inactivation device 20 is installed in the chamber 1 is measured. Further, in order to evaluate the performance of the inactivation apparatus 20, in addition to the quantitative analysis of the infectious titer, the quantitative analysis of the number of virus particles by the quantitative RT-PCR method is performed in parallel (step 201).

3 (a) and 3 (b), the same air collection bag 6 is reused. However, when the time interval of air collection is short or when it is desired to improve the cycle time, FIG. The procedure may be as in d), and a new bag may be replaced each time air is collected by the air collection bag 6.

As shown in FIG. 3C, the processes of steps 101, 102, 103, and 104 are performed in the same manner as in FIG. The following process is performed after the process of step 104 is completed.

(Installation of new air sampling bag 6)
When the air collection bag 6 from which the air has been sucked is removed from the chamber 1 (step 104), another new air collection bag 6 is attached to the chamber 1 (step 105 '). Thereafter, the procedure returns to step 102 and the same process is repeated a predetermined number of cycles.

(Capture virus in the filter for capture)
On the other hand, the air collection bag 6 removed from the chamber 1 is equipped with a suction device 11 shown in FIG. As a result, the suctioned air passes through the capture filter 10 and the floating infectious influenza virus in the air collection bag 6 is captured by the capture filter 10 (step 201 ′).

(Quantitative analysis)
Next, the floating infectious influenza virus is recovered from the capture filter 10. Then, using the collected floating infectious influenza virus as a sample, quantitative analysis of the infectious titer is performed. By performing this quantitative analysis on each capture filter 10, the change over time of the infectious titer of the floating infectious influenza virus when the inactivation device 20 is installed in the chamber 1 is measured. Further, in order to evaluate the performance of the inactivation apparatus 20, in addition to the quantitative analysis of the infectious titer, the quantitative analysis of the number of virus particles by the quantitative RT-PCR method is performed in parallel (step 202 ′).

Example 1
An example of the above-described first embodiment will be described.

(Measurement target)
As an inactivation device 20 to be measured, O.D. T.A. A SC125-TB air cleaner manufactured by Company A was used. This air cleaner is an air cleaner that takes in outside air and discharges air that has passed through a photocatalytic filter to the outside.

A housing of the air cleaner contains a photocatalytic filter in which a porous ceramic body is used as a base material and an ultraviolet responsive photocatalytic layer such as titanium dioxide is formed on the surface thereof. In addition, an ultraviolet lamp is housed in the housing for irradiating the photocatalyst with ultraviolet rays to excite the photocatalyst and oxidatively decompose the floating infectious influenza virus. The housing also houses a suction fan for taking in the outside air and allowing the air to pass through the photocatalytic filter to be discharged outside. In addition, a drive control device and the like for driving and controlling the air cleaner are incorporated in the housing.

This air cleaner is characterized in that the particles of the active layer of titanium oxide constituting the photocatalyst layer are fine and highly active. That is, the specific surface area of titanium oxide is 18.58 m <2> / g, which is very high compared to the specific surface area of a general air cleaner (for example, 6.65 m <2> / g). Therefore, it is thought that the ability to capture airborne infectious influenza virus with a photocatalytic filter and oxidatively decompose and inactivate it is very high.

During the measurement, the air purifier 20 was kept operating with an AC power supply, and the photocatalyst was excited and air was taken in and out by the suction fan.

(Chamber 1, air introduction bag 5, air sampling bag 6)
The length of one side of the chamber 1 was 91 cm, and the chamber 1 was configured as a cubic housing having a volume of 91 cm × 91 cm × 91 cm = 753.57 L (about 0.75 cubic rice).

  The capacities of the air introduction bag 5 and the air sampling bag 6 were 40 L (liters), respectively. Thereby, 40 L of air in the chamber 1 can be sampled in one air sampling cycle.

(Virus injection amount)
10 ml of the virus solution of the floating infectious influenza virus A / PR / 8/34 (H1N1) strain was sprayed from the virus injection port 2 into the chamber 1. The whole amount of the virus solution was sprayed over about 1 minute. The virus titer (infection titer) at the start of spraying was 4.0 × 10 8 PFU / 10 ml.
(Air sampling time interval)
At the time of virus injection completion (at the end of virus spraying), 5 minutes after virus injection completion, 15 minutes after virus injection completion, and 30 minutes after virus injection completion, 40 L of air from inside chamber 1 Collected.

(Capture filter 10)
Using the capture filter 10 with a pore size of 0.45 μm, airborne infectious influenza virus was aspirated and captured from 40 L of collected air, and collected as a sample for quantitative analysis. An experiment was conducted in comparison with a trapping filter having a pore size of 0.2 μm, and a pore size of 0.45 μm was optimal in terms of suction speed and recovery rate.

  A membrane filter (0.45 μm cellulose acetate membrane φ47 mm) was used as the capturing filter 10. When the virus solution was dropped onto the membrane filter and allowed to permeate the culture solution to collect infectious virus, about 10% of the dropped virus could be collected. As a result, it was confirmed that if the membrane filter is the capturing filter 10, infectious virus can be efficiently recovered from the collected air.

(Quantitative analysis)
Quantitative analysis was performed for both infection titer and virus particle count.

  Immediately after aspirating the collected air, the capture filter 10 is cut in half, and one is placed in the RNA extraction lysate to serve as a sample for analysis of the number of virus particles and the other is used for analysis of infectious titer To make a sample, it was suspended in 2 ml of cell culture medium.

(Infectious titer analysis)
Virus captured by half of the capture filter 10 was suspended in the cell culture medium, serially diluted, inoculated into MDCK cells, and agarose was overlaid. The plaques thus formed were stained, the number of plaques was counted, and the infectious virus titer (unit: PFU) in 40 L of collected air was calculated (quantification by plaque method).

(Analysis of the number of virus particles)
The virus captured by half of the capture filter 10 is put into an RNA extraction lysate, the RNA of the virus is extracted, and the number of RNA copies in 40 L of collected air is obtained by RT-PCR and quantitative PCR methods targeting HA. (Unit: number of copies) was calculated.

  In addition, according to the experiment, it was confirmed that the infectious virus titer can be detected even if the RNA copy number is below the detection sensitivity. Therefore, the employed infectious titer measurement method is superior to the nucleic acid detection method. Thus, the infectivity measurement sensitivity is higher than the genome measurement sensitivity, and is considered to be optimal for the measurement of airborne infectious influenza virus.

(Contrast)
In order to compare the inactivation effect when the air cleaner 20 is installed in the chamber 1 with reference to the case where the air cleaner 20 is not installed, the air cleaner 20 is installed when the air cleaner 20 is installed. In both cases where 20 was not installed, measurement processing was performed according to the procedure shown in FIGS. 3 (a), 3 (b) or 3 (c), (d). However, when the air cleaner 20 was not installed in the chamber 1, a small fan was installed to convect the air in the chamber 1.

(Measurement result)
FIG. 4 shows the change over time (solid line) in the infectious titer of airborne infectious influenza virus when the air cleaner 20 is installed in the chamber 1, and the case where the air cleaner 20 is not installed in the chamber 1. The change over time (broken line) of the infectious titer of airborne infectious influenza virus is shown in comparison.

In FIG. 4, the horizontal axis is the elapsed time (minutes), and the vertical axis is the infectious titer (PFU) of the floating infectious influenza virus. Each plot point is at the time of virus injection completion (horizontal axis “0 minutes”), 5 minutes after virus injection completion (horizontal axis “5 minutes”), and 15 minutes after virus injection completion (horizontal axis “15 minutes”). ", Corresponding to 30 minutes after the completion of virus injection (horizontal axis" 30 minutes ").

As is clear from FIG. 4, when the air cleaner 20 is not installed in the chamber 1 (broken line), the infectious titer is sufficiently high even after 30 minutes, and the floating infectious influenza in the chamber 1 Although the virus has not been inactivated, when the air cleaner 20 is installed in the chamber 1 (solid line), the infectious titer has already reached the detection limit and has floated in the chamber 1 after 5 minutes. It can be seen that the infectious influenza virus has been dramatically inactivated.

From the above, it was found that the air cleaner 20 to be measured has a very high effect of inactivating the airborne infectious influenza virus in the air.

On the other hand, in the analysis result of the number of virus particles, a detection result of about 10,000 copies / 40 L was obtained after 5 minutes. This means that there are approximately 190,000 virus particles in the chamber 1 when the number of virus particles including both inactivated and non-inactivated viruses has elapsed for 5 minutes. Therefore, it can be evaluated that the air cleaner 20 to be measured has not only the ability to capture and remove viruses but also the ability to decompose and kill viruses.

4 shows the change over time of the infectious titer of the floating infectious influenza virus when the air cleaner 20 is installed in the chamber 1, and the floating when the air cleaner 20 is not installed in the chamber 1. The change over time of the infectious titer of infectious influenza virus is measured and compared with each other. Similarly, the change over time in the number of virus particles when the air cleaner 20 is installed in the chamber 1 and the air cleaning If the machine 20 is not installed in the chamber 1, the time-dependent change in the number of virus particles may be measured and compared.

(Example 2)
In the first embodiment, the air purifier has been described as an example of the inactivation device 20.

However, the inactivation device 20 can naturally be applied to a device which generates an ionization phenomenon in the atmosphere to generate positive ions and negative ions.

(Example 3)
Inhabiting organisms that can be infected with airborne infectious influenza virus, such as mice, are placed in the chamber 1, and an inactivation device 20 such as an air purifier is installed. It is possible to carry out measurement.

In this case, a mouse-adapted virus strain, for example, a mouse-adapted influenza virus A / PR8 / 34 strain is injected from the virus injection port 2. On the other hand, a plurality of mice are placed in the chamber 1. Then, after a predetermined time has elapsed since the floating infectious influenza virus was injected into the chamber 1, the mouse is taken out of the chamber 1 and collected. Next, tests such as mortality due to mouse infection, presence or absence of mine production, and immunopathological analysis of lungs are performed. From these test results, the infectivity reduction effect of the floating infectious influenza virus when the inactivation device 20 is installed is measured. The infectivity reduction effect of the floating infectious influenza virus when the inactivation device 20 is not installed may also be measured, and the two measurement results may be compared.

In addition, it is desirable to carry out this measurement in equipment that conforms to the specifications of infected animal laboratories (P3 specifications), for example, in a safety cabinet.

Example 4
In the above description, it is assumed that the inactivation effect of the inactivation device 20 such as an air purifier is measured.

  However, when an inactivating agent that inactivates airborne infectious influenza virus is sprayed using the same measuring device and measuring method, it is also possible to measure the effect of inactivating the infectious influenza virus by this inactivating agent. Is possible.

  In this case, the floating infectious influenza virus is sprayed into the chamber 1 from the virus injection port 2 as in the first embodiment. On the other hand, before or after virus injection, an inactivating agent that inactivates the floating infectious influenza virus is sprayed from the virus injection port 2. Note that a port for injecting the inactivating agent may be provided separately from the virus injection port 2. Thereafter, the processing after step 102 shown in FIG. 3 is performed in the same manner, and the change over time in the infectious titer of the floating infectious influenza virus is measured. It is also possible to measure the change over time in the infectious titer when the inactivating agent is not sprayed, and to compare both measurement results.

(Second Embodiment)
In the first embodiment, an inactivation device 20 such as an air purifier installed indoors or an inactivation agent sprayed indoors is assumed. However, it is also possible to measure the inactivation effect of the filter provided in the indoor air introduction path or the outdoor air exhaust path.

  Here, the air introduction path to the room or the air exhaust path to the outside is, for example, an air intake of a building of a general household room, a factory, a building, a ventilation fan, an exhaust duct of an indoor or automobile air conditioner, etc. is there. The filter is a filter that captures and inactivates at least the suspended infectious influenza virus in the introduction air or the exhaust air, and may or may not have a photocatalytic layer (referred to herein as an inactivation filter). Therefore, the inactivation filter includes a photocatalytic filter that can obtain an oxidative decomposition effect by the photocatalyst.

FIG. 5 shows a configuration of a measuring apparatus according to the second embodiment.

The inactivation filter 41 to be measured according to the embodiment is configured in a plate shape and has a front surface and a back surface. The inactivation filter 41 is configured as an inactivation filter unit 40 including a fan 42 (for example, an electric fan) forcing air into the inactivation filter 41 and a frame 43 that holds the periphery of the inactivation filter 41. It is assumed that the product has been commercialized so that it can be mounted in the above-described outdoor air exhaust path. One surface 40 a of the inactivating filter unit 40 corresponds to the surface of the inactivating filter 41, and the other surface 40 b of the inactivating filter unit 40 corresponds to the back surface of the inactivating filter 41.

The air generation means 31 generates air containing airborne infectious influenza virus. For example, when the floating infectious influenza virus used for measurement is stored in the state of a preservation solution, a device that makes the preservation solution into an atomized state is used. For example, an ultrasonic atomizer can be used. For example, a commercially available ultrasonic spray generator (registered trademark “nebulizer”) can be used.

The air generating means 31 and the one surface 40 a of the inactivating filter unit 40 are communicated with each other through the suction passage 32.

A chamber 33 is provided on the other surface 40 b side of the inactivation filter unit 40. The other surface 40 b of the inactivating filter unit 40 and the chamber 33 are communicated with each other by an exhaust passage 34.

In the chamber 33, a living organism that can be infected with airborne infectious influenza virus, such as a mouse, is placed. Accordingly, the air generated by the air generating means 31 includes a mouse conditioned virus strain, for example, a mouse conditioned influenza virus A / PR8 / 34 strain. On the other hand, a plurality of mice are placed in the chamber 31. In addition, it is desirable to perform the measurement by this measuring device in the equipment that conforms to the specification (P3 specification) of the infected animal laboratory, for example, in a safety cabinet.

The chamber 33 is provided with an exhaust port 33 a for exhausting the air in the chamber 33.

An air sampling bag 6 similar to that shown in FIG. 1 is connected to the exhaust port 33a.

The air sampling bag 6 removed from the chamber 33 is attached to the same suction device 11 as shown in FIG. 1B, and air is sucked.

The fan 42 constituting the inactivated filter unit 40 sucks air containing floating infectious influenza virus generated by the air generating means 31 from one surface 40a of the inactivated filter unit 40 through the suction passage 32, and this The suction / exhaust means is configured to exhaust air from the other surface 40 b of the inactivation filter 40 through the exhaust passage 34 and introduce the exhaust gas into the chamber 33. If the inactivation filter unit 40 does not include the fan 42, the same suction / exhaust means is separately provided on the one surface 40a side or the other surface 40b side of the inactivation filter unit 40 to force Further, the inactivation filter 41 is configured to take in air.

Next, the procedure of the measurement method performed by the measurement apparatus described above will be described. As in the first embodiment, it is necessary to appropriately seal each part so that no leakage of air containing viruses occurs during measurement.

First, the fan which comprises the inactivation filter unit 40 is operated.

Next, the air generating means 31 is operated. Thereby, the air containing the floating infectious influenza virus generated by the air generating means 31 is sucked from the one surface 40 a of the inactivated filter unit 40 through the suction passage 32. Then, air is exhausted from the other surface 40 b of the inactivating filter 40 through the exhaust passage 34 and introduced into the chamber 33.

Air is exhausted from the exhaust port 33 a of the chamber 33 and collected by the air collection bag 6.

After the air generation means 31 starts generating air containing airborne infectious influenza virus, the mouse is taken out of the chamber 33 and collected after a predetermined time has elapsed. Next, tests such as mortality due to mouse infection, presence or absence of mine production, and immunopathological analysis of lungs are performed. From these test results, the infectivity reduction effect of the floating infectious influenza virus when the inactivated filter unit 40 is installed is measured. The infectivity reduction effect of the floating infectious influenza virus when the inactivating filter unit 40 is not installed may also be measured, and the two measurement results may be compared. When the inactivating filter unit 40 is not installed, it is desirable to install only the fan 42 at the corresponding location so that air containing the floating infectious influenza virus is forcibly introduced into the chamber 33.

Alternatively, inactivation filters with different specifications may be sequentially replaced to perform similar mouse inspections and measurements based on the inspection results, and compare the infectivity reduction effects of inactivation filters with different specifications. Thereby, the evaluation which contrasted the inactivation filter from which each specification differs can be performed.

While the mouse is carried out and collected, the air collection bag 6 is removed from the chamber 33 after a predetermined time has elapsed since the air generation means 31 started to generate air containing floating infectious influenza virus.

A suction device 11 shown in FIG. 1B is attached to the air sampling bag 6 removed from the chamber 1 to suck air. As a result, the suction air passes through the capture filter 10, and the floating infectious influenza virus in the air collection bag 6 is captured by the capture filter 10.

Next, airborne infectious influenza virus is collected from the capture filter 10, and the infectious titer and the number of virus particles are quantitatively analyzed using the collected airborne infectious influenza virus as a sample. Thereby, the infectious titer and the number of virus particles of the floating infectious influenza virus when the inactivating filter unit 40 is installed are measured. Only the infectious titer of airborne infectious influenza virus may be measured, or only the number of virus particles may be measured.

  The performance of the inactivated filter unit 40 can be evaluated by comparing the test result of the mouse with the measurement result of the infectious titer of the floating infectious influenza virus and / or the number of virus particles thus obtained.

  In addition, the inactivation filter unit 40 is removed after a predetermined time from the start of generation of air containing airborne infectious influenza virus by the air generating means 31, the airborne infectious influenza virus is recovered from the inactivated filter, and is similarly suspended. The infectious influenza virus infection titer or / and the number of virus particles may be measured. Then, the infectious titer measured based on the floating infectious influenza virus collected from the inactivation filter 41 and / or the measurement result of the number of virus particles is added to the mouse test result and the floating infectivity collected from the capture filter 10. The performance of the inactivated filter unit 40 can be evaluated by comparing the infectious titer measured based on the influenza virus and / or the measurement result of the number of virus particles.

  In the measuring apparatus shown in FIG. 5, the air collection bag 6 is connected to the exhaust port 33a of the chamber 33. Instead of connecting the air collection bag 6, the trapping filter 10 is provided at the exhaust port 33a. Implementation is also possible. Thus, the floating infectious influenza virus can be collected from the capture filter 10 without performing the step of sucking air from the air collection bag 6.

  Further, in the measuring apparatus shown in FIG. 5, the air that has passed through the inactivating filter unit 40 is introduced into the chamber 33 and introduced into the air sampling bag 6, but as shown in FIG. It is also possible to perform measurement based on the test result of the mouse in the chamber 33 without providing the air collection bag 6. Further, as shown in FIG. 2C, it is also possible to perform measurement based on the collected air of the air collection bag 6 without providing the chamber 33.

1, 33 chamber, 2 virus injection port, 3 air introduction port, 4 air exhaust port, 5 air introduction bag, 5 air collection bag, 10 trapping filter, 20 inactivation device, 31 air generation means, 32 suction passage 34 Exhaust passage, 40 Deactivation filter unit, 41 Deactivation filter

Claims (15)

When an inactivation device that inactivates airborne infectious influenza virus is installed in the chamber, the method for measuring the inactivation effect of airborne infectious influenza virus by the inactivation device,
Inject a predetermined amount of airborne infectious influenza virus into the chamber,
An air introduction step for introducing a predetermined volume of air into the chamber;
A predetermined time interval is obtained by repeatedly performing each step consisting of an air sampling step for sampling a predetermined volume of air exhausted from the chamber in response to the predetermined volume of air being introduced into the chamber. To collect a predetermined volume of air from inside the chamber,
A process of passing the collected predetermined volume of air through a filter capable of capturing airborne infectious influenza virus is performed for each of the predetermined volume of air collected at predetermined time intervals,
Quantitative analysis of the infectious titer of airborne infectious influenza virus trapped in each filter to measure the time course of the infectious titer of airborne infectious influenza virus when the inactivation device is installed in the chamber The measuring method of the inactivation effect of the floating infectious influenza virus characterized by the above-mentioned. By performing quantitative analysis of the infectious titer of airborne infectious influenza virus trapped in each filter for the case where the inactivation device is installed in the chamber and the case where the inactivation device is not installed, the inactivation device is placed in the chamber. Measuring the time course of the infectious titer of airborne infectious influenza virus when installed in the chamber and the time course of the infectious titer of airborne infectious influenza virus when the inactivation device is not installed in the chamber The method for measuring the effect of inactivating the airborne infectious influenza virus according to claim 1. The inactivation device is an air purifier that takes in outside air and discharges air that has passed through a virus inactivation filter including a photocatalytic filter to the outside. Measurement method. Furthermore, we perform quantitative analysis of the number of floating infectious influenza virus particles trapped by the filter, and evaluate the performance of the inactivation device by combining the quantitative analysis result of the virus particle number with the quantitative analysis result of the infectious titer. The method for measuring the effect of inactivating the floating infectious influenza virus according to claim 1, 2 or 3. 2. The method for measuring the inactivation effect of airborne infectious influenza virus according to claim 1, wherein the inactivation apparatus is an apparatus which generates an ionization phenomenon in the atmosphere to generate positive ions and negative ions. An organism capable of being infected with airborne infectious influenza virus is allowed to remain in the chamber, and after a predetermined time has elapsed since the airborne infectious influenza virus was injected into the chamber, the organism was recovered from the chamber, and the organism was recovered. The method for measuring the effect of inactivating the infectious influenza virus according to claim 1, wherein the effect of reducing the infectivity of the infectious influenza virus when the inactivation device is installed is measured. When an inactivating agent that inactivates airborne infectious influenza virus is sprayed in the chamber, the method for measuring the inactivating effect of airborne infectious influenza virus by the inactivating agent,
Inject a predetermined amount of airborne infectious influenza virus into the chamber,
An air introduction step for introducing a predetermined volume of air into the chamber;
A predetermined time interval is obtained by repeatedly performing each step consisting of an air sampling step for sampling a predetermined volume of air exhausted from the chamber in response to the predetermined volume of air being introduced into the chamber. To collect a predetermined volume of air from inside the chamber,
A process of passing the collected predetermined volume of air through a filter capable of capturing airborne infectious influenza virus is performed for each of the predetermined volume of air collected at predetermined time intervals,
Quantitative analysis of the infectious titer of airborne infectious influenza virus trapped in each filter to measure the time course of the infectious titer of airborne infectious influenza virus when the inactivating agent is sprayed into the chamber The measuring method of the inactivation effect of the floating infectious influenza virus characterized by the above-mentioned. When an inactivation device that inactivates airborne infectious influenza virus is installed in the chamber, the device for measuring the inactivation effect of airborne infectious influenza virus by the inactivation device,
A virus inlet provided in the chamber for injecting a predetermined amount of floating infectious influenza virus into the chamber;
An air inlet for introducing air into the chamber;
A predetermined volume of air introduction bag into which air is pushed into the chamber through the air introduction port;
An air exhaust port through which air is exhausted from the chamber as the predetermined volume of air is pushed into the chamber through the air inlet and introduced into the chamber;
An air sampling bag for collecting air exhausted through the air exhaust port;
A suction means for sucking a predetermined volume of air collected in the air collection bag through the suction passage;
A filter provided on the suction passage and capable of capturing a floating infectious influenza virus;
Collect a predetermined volume of air with a bag for collecting air at a predetermined time interval,
The collected predetermined volume of air is sucked by the suction means,
Quantitative analysis of the infectious titer of airborne infectious influenza virus trapped in each filter to measure the time course of the infectious titer of airborne infectious influenza virus when the inactivation device is installed in the chamber A measuring device for inactivating effect of airborne infectious influenza virus, characterized in that. When an inactivating agent that inactivates airborne infectious influenza virus is sprayed in the chamber, the device for measuring the inactivating effect of airborne infectious influenza virus by the inactive agent,
A virus inlet provided in the chamber for injecting a predetermined amount of floating infectious influenza virus into the chamber;
An air inlet for introducing air into the chamber;
A predetermined volume of air introduction bag into which air is pushed into the chamber through the air introduction port;
An air exhaust port through which air is exhausted from the chamber as the predetermined volume of air is pushed into the chamber through the air inlet and introduced into the chamber;
An air sampling bag for collecting air exhausted through the air exhaust port;
A suction means for sucking a predetermined volume of air collected in the air collection bag through the suction passage;
A filter provided on the suction passage and capable of capturing a floating infectious influenza virus;
Collect a predetermined volume of air with a bag for collecting air at a predetermined time interval,
The collected predetermined volume of air is sucked by the suction means,
Quantitative analysis of the infectious titer of airborne influenza virus trapped in each filter to measure changes over time in the infectious titer of airborne infectious influenza virus when an inactivating agent is installed in the chamber A measuring device for inactivating effect of airborne infectious influenza virus, characterized in that. A method of measuring the inactivation effect of airborne infectious influenza virus by an inactivation filter that captures and inactivates airborne infectious influenza virus in introduced air or exhaust air,
Air containing airborne infectious influenza virus is inhaled from one side of the inactivation filter, exhausted from the other side of the inactivation filter,
Collect a predetermined volume of air exhausted from the other surface of the inactivation filter,
The collected volume of air is passed through a capture filter that can capture airborne influenza viruses,
A floating infectious influenza virus characterized by evaluating the performance of the inactivating filter by quantitatively analyzing the infectious titer or / and the number of virus particles of the floating infectious influenza virus captured by the capture filter. Measurement method of inactivation effect. A method of measuring the inactivation effect of airborne infectious influenza virus by an inactivation filter that captures and inactivates airborne infectious influenza virus in introduced air or exhaust air,
Air containing airborne infectious influenza virus is inhaled from one side of the inactivation filter, exhausted from the other side of the inactivation filter,
Introducing the air exhausted from the other surface of the inactivation filter into a chamber in which a living organism capable of infecting airborne infectious influenza virus is located;
After a predetermined time has elapsed since air containing airborne infectious influenza virus was introduced into the chamber, the life form was collected from the chamber, and the life form was inspected. A method for measuring the inactivation effect of airborne infectious influenza virus characterized by measuring the effect of reducing infectivity. Exhaust the air in the chamber from the outlet of the chamber;
The air exhausted from the chamber is passed through a capture filter capable of capturing airborne infectious influenza virus;
12. The performance of the inactivation filter is evaluated by performing a quantitative analysis of an infectious titer of floating infectious influenza virus captured by the capture filter and / or the number of virus particles. A method for measuring the inactivation effect of airborne infectious influenza virus. In a device that measures the inactivation effect of airborne infectious influenza virus by an inactivation filter that captures and inactivates airborne infectious influenza virus in the introduction air or exhaust air,
Air generating means for generating air containing airborne infectious influenza virus;
A suction passage communicating the air generating means and one surface of the inactivation filter;
A collection bag of a predetermined capacity provided on the other surface side of the inactivation filter, for collecting air exhausted from the other surface of the inactivation filter;
An exhaust passage communicating the other surface of the inactivation filter and the collection bag;
Inhalation / exhaust means for inhaling air containing airborne infectious influenza virus from one surface of the inactivation filter through the inhalation passage and exhausting from the other surface of the inactivation filter through the exhaust passage. ,
An air sampling bag for collecting air exhausted through the exhaust passage;
A suction means for sucking a predetermined volume of air collected in the air collection bag through the suction passage;
Provided on the suction passage, and provided with a capture filter capable of capturing floating infectious influenza virus,
A floating infectious influenza virus characterized by evaluating the performance of the inactivating filter by quantitatively analyzing the infectious titer or / and the number of virus particles of the floating infectious influenza virus captured by the capture filter. Inactivation effect measuring device. In a device that measures the inactivation effect of airborne infectious influenza virus by an inactivation filter that captures and inactivates airborne infectious influenza virus in the introduction air or exhaust air,
Air generating means for generating air containing airborne infectious influenza virus;
A suction passage communicating the air generating means and one surface of the inactivation filter;
A chamber that is provided on the other surface side of the inactivation filter and into which air exhausted from the other surface of the inactivation filter is introduced, in which a living organism that can be infected with airborne infectious influenza virus is located A chamber;
An exhaust passage communicating the other surface of the inactivation filter and the chamber;
Inhalation / exhaust means for inhaling air containing airborne infectious influenza virus from one surface of the inactivation filter through the inhalation passage and exhausting from the other surface of the inactivation filter through the exhaust passage. Is provided,
After a predetermined time has elapsed since air containing airborne infectious influenza virus was introduced into the chamber, the life form was collected from the chamber, and the life form was inspected. A measuring device for inactivating effect of airborne infectious influenza virus characterized by measuring infectivity reduction effect. An exhaust port provided in the chamber for exhausting air in the chamber;
An air sampling bag for collecting air exhausted through the exhaust port;
A suction means for sucking a predetermined volume of air collected in the air collection bag through the suction passage;
Provided on the suction passage, and provided with a capture filter capable of capturing floating infectious influenza virus,
15. The performance of the inactivation filter is evaluated by performing a quantitative analysis of the infectious titer or / and the number of virus particles of airborne infectious influenza virus captured by the capture filter. Measuring device for inactivation effect of airborne infectious influenza virus.

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