TW200403081A - Method of estimating elimination of microorganisms and apparatus for estimating elimination of microorganisms - Google Patents

Abstract

It is intended to estimate the antimicrobial effect of a sterilization treatment whereby microorganisms are irradiated with particles. Microorganisms are introduced into the space in a container (8) and then irradiated with particles (7) for sterilization. After the completion of the irradiation with the particles (7), the microorganisms are collected into a collection container (6) and measured to thereby estimate the effect. The microorganisms being sterilized may be one or more members selected form the group consisting of bacteria, fungi, viruses and allergens. As the particles, use can be made of cations, anions, a gas containing a mixture of cations with anions, charged particles such as α - and β -rays, various plasmatic gas ions, ozone and radical particles, drug particles and so on.

Description

200403081 (1) · Description of the invention [Technical field to which the invention belongs] The present invention relates to a method for assessing the removal of microorganisms and a device for assessing the removal of microorganisms for evaluating the bactericidal effect of space plankton. [Previous technology]

In recent years, with the high air-tightness of the living environment, the requirement to remove planktonic microorganisms in the air harmful to the human body to live a healthy and fast life has gradually increased. In response to this demand, we have developed filters with various antibacterial agents. [Summary of the Invention] Problems to be Solved by the Invention However, because the above-mentioned filter attracts the air in the space and filters microorganisms in the air, after long-term use, the maintenance of the exchange filter is indispensable, and filtering The characteristics of the device are not sufficient, and satisfactory performance cannot be obtained, which is not enough as a way to remove microorganisms. Lu Therefore, the evaluation of the removal of planktonic microorganisms is carried out, and the air containing microorganisms is passed through the filter, and the number of microorganisms filtered by the filter is determined. In this method, the concentration of planktonic microorganisms in the target space cannot be measured. However, there is a method for removing microorganisms by sterilizing the microorganisms by irradiating particles such as ionized ions, and measuring the removal ability of the microorganisms by sterilizing treatment in this way has never been performed before. Here, the present invention is to provide microbes, especially viruses, with sterilization treatment particles, especially ionic particles formed by positive and negative ions. ½¾. 7-(2) (2) 200403081 shot on microorganisms, A method for evaluating the removal of microorganisms for evaluating its bactericidal effect and a device for evaluating the removal of microorganisms using the method are intended. Means for solving the problem In order to solve the above-mentioned problem, the present invention is a method for evaluating the removal of microorganisms by supplying microorganisms to the internal space of the container and simultaneously irradiating particles formed by positive and negative ions used for sterilizing the microorganisms. It adopts microorganisms, and it is characterized by the measurement of the adopted microorganisms. In addition, in the present invention, in order to evaluate the method for removing viruses, a virus as a microorganism is supplied to the inner space of the container, and particles for sterilizing the virus are irradiated. After the particles are irradiated, the virus is taken to measure the virus taken. According to this method, after the above particles are irradiated in the container's internal space, microorganisms are used to perform the measurement. Therefore, the ability to sterilize microorganisms by irradiating the particles can be evaluated, and various conditions for irradiating the particles can be quantitatively evaluated. Therefore, in the method for evaluating the removal of microorganisms, after the irradiation of the particles, the measurement of the microorganisms is performed, and further, the microorganisms are supplied under the same conditions as when the particles are irradiated to sterilize the microorganisms, and the particles are not irradiated, so that After the microorganisms have naturally decayed, the microorganisms are taken and the determination of the taken microorganisms is performed. That is, the invention irradiates the above-mentioned particles for a certain period of time to sterilize the microorganisms, and supplies the microorganisms under the same conditions as when the microorganisms are sterilized to treat the microorganisms for the same time as the particles, without irradiating the particles, so that the microorganisms are natural. After the decline, take the microorganisms and perform the measurement of (3) (3) 200403081 microorganisms. Therefore, 'to measure the microorganisms that were sterilized by irradiating the above-mentioned particles and the microorganisms that were naturally degraded without the relevant sterilization treatment, and compare the results. Based on the comparison of the ability of the microorganisms to be sterilized by irradiating the above-mentioned particles and the natural decay, A relative assessment can be made. The measurement of the microorganism is the measurement of the concentration of the microorganism, the measurement of the cell infection rate, or the measurement of the allergic reaction, and the removal of microorganisms can be evaluated. In addition, the measurement of the microorganisms collected as described above can be used to measure the change over time of the irradiation time of the particles. Based on this, the elapsed time of the ability to sterilize the microorganism can be quantitatively evaluated. In addition, for measuring the microorganisms collected as described above, the concentration dependence of the particles can also be measured. Based on this, the concentration dependence of the ability to sterilize the microorganisms can be quantitatively evaluated. The supply of microorganisms to the inner space of the container is a solution in which the microorganisms are dispersed and sprayed in a mist form. Accordingly, the microorganisms can be easily supplied into the container, and the microorganisms can be easily sterilized. Secondly, when the relevant microorganisms are sprayed in the form of a mist, they can be evaluated by the present invention. In addition, the above evaluation method can be used for cell culture of microorganisms, erythrocyte agglutination reaction of microorganisms, or allergic reactions of microorganisms. Accordingly, the activity or concentration of microorganisms can be evaluated. In addition, as the particles for sterilizing the microorganisms described above, any of the gas generated by air discharge, radiation irradiation in the air, and Lenard effect can be used. 200403081 ⑷ In addition, as the particles for sterilizing the microorganisms mentioned above, radiation, X-ray, 7-ray or electromagnetic wave can be used. In addition, as the particles for sterilizing the microorganism, positive and / or negative ions may be used. Here, the reason for the sterilization of microorganisms when using positive and negative ions as special particles for sterilization of microorganisms is as follows. That is, the ionization phenomenon such as electric discharge is caused in the air, so that when positive ions and negative ions occur, 最 + (Η20) η, which is the most stable ions, and 02— (Η20) η, which is the negative ions, are generated. _ When these ions are generated, hydrogen peroxide Η 202 or free radical · ΟΗ, which is an active source due to the chemical reaction, because the Η 202 or free radical · Η Η exhibits extremely strong activity, which can be sterilized to remove airborne particles. Microorganisms. As the particles for sterilizing the microorganisms, a gas mainly composed of either positive or negative ions can be used. In this case, according to the charge of the above-mentioned ions, the electric action on the microorganisms is to destroy the microbial cells or the surface proteins to obtain the effect of generating a bactericidal effect. In addition, the use of a medicament for sterilizing the microorganisms is also used. The medicine particles can be irradiated for sterilization treatment. In the case of a sterilization treatment using a chemical agent, the particles can be supplied in a simpler device as compared with the case of ionization or ozone. Therefore, the ability of related agents to sterilize microorganisms can also be evaluated. ~ In addition, as the microorganism to be subjected to the sterilization treatment, the microorganism may be a group of at least one or a combination of two or more selected from the group consisting of bacteria, fungi, viruses, and allergens. Based on this, various microorganisms can be the subject of the evaluation of the present invention -10- (5) (5) 200403081. When the microorganisms are supplied to the inner space of the container, the inner space of the container can be stirred from the lower side with respect to the microorganisms supplied in the container. According to this, the supply of microorganisms into the container can prevent the microorganisms from naturally precipitating due to their own weight and irradiate the particles to effectively perform sterilization. In addition, when it is agitated, it may be an evaluation target of the present invention. In addition, the present invention provides a device for realizing the above-mentioned evaluation of the removal of microorganisms, including an internal space capable of supplying microorganisms, a container for sterilizing the microorganisms, and a method for supplying microorganisms to the internal spaces of the containers. And the microorganisms removal means for supplying the particles for sterilizing the microorganisms in the inner space of the container, and after the microorganisms are sterilized by the microorganisms removing means, the microorganisms are taken by the microorganisms, and the microorganisms taken by the microorganisms are measured. The device used for evaluation is to evaluate the removal of microorganisms. According to the apparatus for evaluating the removal of microorganisms, after the microorganisms are irradiated with the above-mentioned particles for the sterilization of microorganisms by the above-mentioned microorganisms removing means, the microorganisms are taken by the above-mentioned microorganisms taking means, and the measured microorganisms are measured. Based on the measurement, the above-mentioned microorganisms removing means can be evaluated Ability to sterilize microorganisms. In addition, various conditions for sterilizing microorganisms by irradiating particles in accordance with the above-mentioned microorganism removal means can be quantitatively evaluated. As a specific form, the microbial supply means, the microbial removal means, and the microbial take means are connected to the air passage containing the microorganisms, and are arranged in order from the upstream side to the downstream side. According to this, a series of steps of microbial supply, microbial removal, and microbial collection can be smoothly performed. -11- (6) (6) 200403081 At this time, if a microbial supply means and a microbial take-up means are used to form a wind tunnel containing an air passage containing microorganisms, and the microbial removal means is arranged inside the wind tunnel, it can be contained in the restricted wind tunnel. Supply, removal and collection of air from microorganisms. The microorganism removing means and the microorganism taking means may be arranged in a region other than a region directly below the microorganism supplying means. According to such a configuration, since the gaseous particulate matter falls vertically below and around the mist released by the above-mentioned microorganism supply means, the above-mentioned microorganism removing means and the above-mentioned microorganism taking means are not polluted by the above-mentioned falling substances. , Which can improve the reliability of the evaluation device. In order to obtain this effect, it is important that the microorganism removal means and the microorganism removal means are not disposed vertically below the microorganism supply means, for example, the microorganism removal means and the microorganism removal means are arranged horizontally, or the microorganism supply means This effect can be obtained by a deviation position vertically below, or by disposing the above-mentioned microorganism removal means and the above-mentioned microorganism taking means in an oblique direction. In addition, in the present invention, it is possible to arrange a device for evaluating the removal of microorganisms in other containers that can cover the above-mentioned container on the outer side of the container. With such a device structure, the microorganisms leaked out of the above-mentioned container, or the particulate matter that is not gasified can be shielded by the above-mentioned other containers, and it is difficult to leak from the outside. In addition, regarding the apparatus for evaluating the removal of microorganisms, in the container internal space, the supplied microorganisms may be provided with a stirring means for stirring from below -12-(7) (7) 200403081. Accordingly, when the microorganisms are supplied into the container by the microorganism supply means described above, the microorganisms are prevented from naturally precipitating due to their own weight, and the sterilization means by the microorganism removal means can be effectively performed. In addition, the apparatus for evaluating the removal of microorganisms may be configured to supply microorganisms by the microorganism supply means, so that the solution in which the microorganisms are dispersed is atomized, and sprayed on the inner space of the container. In addition, in the apparatus for evaluating the removal of microorganisms, the particles used for sterilizing the microorganisms may be constituted by the release of gas generated by any of air discharge, radiation in the air, and Lener effect (L e n a r d e f f e c t). In addition, in the apparatus for evaluating the removal of microorganisms, the particles for sterilizing the microorganisms may be any of radiation, X-rays, 7-rays, or electromagnetic waves, and may be constructed by emitting these. In the apparatus for evaluating microorganism removal, the microorganism removal means may be configured to irradiate positive and / or negative ions as particles for sterilization treatment of microorganisms. In the apparatus for evaluating removal of microorganisms, the means for removing microorganisms may be constituted by irradiating pharmaceutical particles as particles for sterilization treatment of microorganisms. Embodiments of the Invention The following describes the embodiments of the present invention. <First Embodiment> First, an apparatus for evaluating the removal of microorganisms capable of carrying out the method of the present invention will be described. Fig. 1 is a schematic diagram showing the evaluation of an example of a device for removing microorganisms. 13- (8) (8) 200403081 A schematic diagram of a device for removing microorganisms. The apparatus 10 for evaluating the removal of microorganisms is provided with a container 8, a microorganism injection tube constituting a microorganism supply means 5, an ion generating device constituting a microorganism removal means 1, a microorganism acquisition tube 3 constituting a microorganism acquisition means, and a microorganism extractor 6. The container 8 has a structure in which the internal space blocks the outside air, so that microorganisms can exist in the internal space and the microorganism can be sterilized. In addition, the container 8 can be arbitrarily adjusted with the temperature or humidity of the internal space by an air conditioning system or the like not shown in the drawing, and the environment for the microorganisms can be arbitrarily set. In addition, as shown in FIG. 1, the thief 8 adopts a form in which the size in the local direction is larger than the size in the horizontal direction. Accordingly, since the volume of the space in the acquisition container 8 can be increased, the processing capacity of the apparatus 10 for evaluating microorganism removal can be increased. The microorganism injection tube 5 is provided at a predetermined position of the container 8. Via the microorganism injection tube 5, the microorganisms can be supplied to the internal space of the container 8, and the microorganisms can float in the internal space of the container 8. The microorganism injection pipe 5 is fed from a microorganism supply source (not shown in the figure). Therefore, the microorganisms are injected into the container 8 from the microorganisms injection port 5 a of the microorganisms injection tube 5 toward the container 8 ^ About the microorganisms injected into the container 8 from the microorganisms injection tube 5, the microorganisms can be injected into the body and the microorganisms can be dispersed The solution was sprayed into a container. The ion generator 1 irradiates ions -14- (9) (9) 200403081 which are particles for sterilization treatment of microorganisms. The ion generating device 1 is installed in the container 8 and faces the microorganisms injected into the container 8 through the microorganism injection port 5a, and the ions 7 are irradiated from the ion generating port 2. The ion generating device 1 is provided with an ion generating element inside thereof. When an AC voltage is applied between the electrodes of the ion generating element, ions 7 formed by positive ions and negative ions are generated due to an ionization phenomenon such as a discharge. Ions 7 generated by the discharge or the like of the related ion generating device 1 are not affected by the state of the gas pressure in the container 8. In addition, the intensity (concentration) of the ions 7 can be adjusted by adjusting the applied voltage to the ion generating element of the ion generating device 1. In the space inside the container 8, a microorganism collecting tube 3 for collecting microorganisms is provided. The take-up tube 3 is constituted by a portion arranged in a vertical direction along the height direction of the container 8 and a portion arranged in a horizontal direction of the container 8 as shown in FIG. 1. Next, a portion arranged along the horizontal direction of the collecting pipe 3 extends through the side surface of the container 8 to the outside of the container 8 and is connected to a microorganism collecting device 6 described later on the outside of the container 8. A microorganism collecting port 3 a is formed at the upper end of the collecting tube 3 in the vertical direction, and the microorganisms in the container 8 are taken into the collecting tube 3 through the collecting port 3 a. The microorganism collecting device 6 is arranged outside the container 8 and the collecting tube 3 constitutes a microorganism collecting device. The microorganisms collector 6 sucks the space in the container 8 through the microorganisms collection tube 3 ', and the microorganisms in the container 8 are taken into the collection tube 3 through the microorganisms collection port 3a and collected in the microorganisms collection device 6. -15-(10) (10) 200403081 The microorganism sampler 6 for collecting microorganisms can be constructed using a gas sampler. The microorganism extractor 6 may be configured to collect microorganisms through a solution foamer. In the apparatus 10 for evaluating the removal of microorganisms, as shown in Fig. 1, a stirrer 4 is provided below the container 8. The agitator 4 is a stirring means for the inner space of the agitating container 8, and it is possible to use a rotating fan to form an air current in the surrounding space to agitate the space. When the stirrer 4 is set to stir the space in the container 8, the microorganisms are prevented from naturally settling down due to their own weight, and the microorganisms can float in the effective existence range of the ions 7 irradiated by the ion generating device 1, which can effectively sterilize the ions deal with. In particular, when the microorganisms are heavy species, natural precipitation is liable to occur, and a stirrer 4 is provided to prevent natural precipitation and to effectively perform sterilization treatment of ions 7. In addition, when the present invention is implemented, it is not necessary to provide the agitator 4. However, when the agitator 4 is provided, the sterilization treatment of the ion 7 can be performed more effectively for the reasons described above. The method for evaluating the removal of microorganisms using the apparatus 10 for evaluating the removal of microorganisms described above can be carried out as described below. First, as described above, a predetermined amount of microorganisms are injected into the container 8 from the microorganism injection port 5. Next, the ion generator 1 is operated to irradiate the implanted microorganisms with ions 7 to sterilize the microorganisms. After a certain period of time, the ions 7 are irradiated, and then the microorganisms are collected by the microorganism collector 6. For the microorganisms taken, the number of bacteria can be determined. About the measurement of microorganisms> 16- (11) (11) 200403081 The number of bacteria can be cultured in a culture dish with a predetermined culture medium for a certain period of time. This allows accurate measurement of the number of microorganisms collected. In addition, for the measurement of the number of microorganisms, the microorganisms on the above-mentioned culture dish can be observed with a microscope. In this way, using the apparatus 10 for evaluating the removal of microorganisms described above, the microorganisms collected by the microorganisms collector 6 can be measured, and the ability to sterilize the microorganisms when the ions 7 are irradiated can be evaluated. In addition, with respect to the microorganism removal evaluation using the apparatus 10 for evaluating microorganism removal described above, the following measurement and evaluation can also be performed. First, as described above, a certain amount of microorganisms are injected into the container 8 and ions 7 are irradiated to perform a sterilization treatment for a certain period of time. After that, the microorganisms are collected by the microorganisms collector 6 to measure the number of the microorganisms. Next, the same amount of microorganisms were injected into the container 8 under the same conditions as the sterilization treatment of the above-mentioned irradiated ion 7. Second, without irradiating the ions 7, the microorganisms naturally decay after the same time as the above-mentioned irradiating ions 7. Thereafter, the microorganisms are collected by the microorganism extractor 6 and the number of bacteria of the collected microorganisms is measured. Secondly, compared with the number of microorganisms used after the above-mentioned sterilization treatment of irradiated ion 7, compared with the number of microorganisms taken after the natural decline, the ionic sterilization treatment ability of the ion 7 with respect to the natural decline can be compared Assessment. In addition, as for the microorganisms collected by the microorganism extractor 6 as described above, it is also possible to measure the change over time of the number of microorganisms -17-(12 (12) 200403081 In addition, when performing the above-mentioned microorganism measurement, the measurement may be performed with stirring by the mixer 4 and without stirring. In addition, when performing the above-mentioned microorganism measurement, the intensity of the ion 7 of the irradiated microorganism may be changed, and the measurement of the microorganisms taken at each intensity of the ion 7 may be performed. Based on this, it is possible to evaluate the sterilization treatment ability of microorganisms in response to the intensity of ion 7. <Second Embodiment> Next, a second embodiment of the apparatus for evaluating microorganism removal according to the present invention will be described with reference to FIG. FIG. 2 is a schematic configuration diagram of a device 20 for evaluating microorganism removal in a second embodiment of the device for evaluating microorganism removal. In the apparatus 20 for evaluating the removal of microorganisms shown in FIG. 2, a container 1 is provided. 8. A microorganism injection tube constituting a microorganism supply means 5. An ion generating element constituting a microorganism removal means 1 2. A collection tube constituting a microorganism removal means 13 和 microbial taking device 6. That is, the microorganism injecting tube 15 of the microorganism supply means, the ion generating element 12 'of the microorganism removing means, and the taking tube 13 and the microorganism extractor 6 of the microorganism taking means are connected to the air passage containing the microorganisms, and are directed from the upstream side toward The downstream side is arranged in order. The internal space of the container 18 is a structure that blocks the outside air so that microorganisms can exist in the internal space and the microorganisms can be sterilized. As can be seen from Fig. 2, the container 18 has a shape in which the size in the height direction is smaller than the size in the horizontal direction. -18-(13) (13) 200403081 The microorganism injection tube 15 is connected to the outside of the container 18, and is connected to the microorganism sprayer 11 and the microorganisms are fed by the microorganism sprayer 11. Microbial nebulizer] 1 is to send microbial gas containing a certain concentration into the microbial injection tube 15 at a certain rate. Next, the microorganism-containing gas system fed into the microorganism injection tube 15 from the microorganism sprayer 11 is injected into the container 18 through the microorganism injection port 15a in the container 18. Regarding the supply of microorganisms into the container 18 by the microbial sprayer 11, the microbial monomers in the air can be sent to the microbial injection tube 15, or the solution of dispersed microorganisms can be misted, and the spray can be fed into the microbial injection tube 15 ° ions The generating element 12 is arranged on the bottom surface inside the container 18 other than the area vertically below the microorganism injection tube 15. The ion generating element 12 is an ion generator 12a arranged in a certain approximately flat shape, and generates ions 7 formed by positive ions and negative ions. The ions 7 generated by the ion generating element 12 process the microorganisms injected by the microbial injection tube 15. The ion generating element 12 is the same as the ion generating element provided in the ion generating device 1 shown in FIG. 1, and generates ions. The operation of 7 is the same as the description of the ion generating device 1. The microbial collection tube 1 3 for collecting microorganisms is arranged outside the area vertically below the microbial injection tube 15 and along the horizontal direction, one end forms a microbial collection port 1 3 a toward the container 18 and the other end is in the container The outside of 18 is connected to the microbial collector 6. The microorganism collecting device 6 arranged outside the container 1 S is a micro-biological (14) (14) 200403081 material collecting tube 13 to attract the space in the container 18, and the microorganisms in the container 18 are collected through the microorganism collecting port 13 a. It is taken into the collection tube 13 and collected in the microorganism collection device 6. As the microbial collector 6 for collecting microorganisms, a gas sampler can be used. The microorganism extractor 6 may be configured to collect microorganisms through a solution foamer. Using the apparatus 20 for evaluating microorganism removal described above, the method of the present invention can be carried out as described below. First, a certain amount of microorganisms are injected into the container 18 through the microorganisms injection port 15. Next, the ion generating element 12 is operated to irradiate the implanted microorganisms with ions 7 to sterilize the microorganisms. After a certain period of time, the ions 7 are irradiated, and then the microorganisms are collected by the microorganism collector 6. Next, the microorganisms collected by the microorganism collector 6 are measured. Regarding the determination of the microorganisms to be taken, the number of the microorganisms to be taken can be determined. As for measuring the number of microorganisms, the collected microorganisms can also be cultured in a Petri dish on a predetermined culture medium for a certain period of time. In addition, the measurement of the number of microorganisms may be observed using a microscope. In this way, the measurement is performed using the apparatus 20 for evaluating the removal of microorganisms, and the microorganisms collected by the microorganisms collector 6 can be used to evaluate the ability to sterilize the microorganisms when the ions 7 are irradiated. In addition, the device 20 for evaluating the removal of microorganisms can inject microorganisms into the container 18 through the microorganism injection port 15 and irradiate the ions 7 of the ion generating element 12 to sterilize the microorganisms. After that, the microorganisms are passed through the microorganisms. Take the mouth 1 3 a to take a series of treatments formed by microorganisms -20-(15) (15) 200403081 An approximate implementation path. Therefore, regarding the apparatus 20 for evaluating microorganism removal, it is not necessary to consider the natural degradation of microorganisms in the container 18, so that the evaluation of removing planktonic microorganisms in the air at a high concentration can be performed. In addition, as for the device 20 for evaluating the removal of microorganisms, the device can be miniaturized and can be evaluated in a confined space, so even harmful microorganisms can be evaluated. In addition, when the method of the present invention is performed using the apparatus 20 for evaluating microorganism removal, the following measurement and evaluation can be performed in the same manner as described when the apparatus 10 for evaluating microorganism removal shown in FIG. 1 is implemented. That is, the microorganisms supplied into the container 18 are naturally degraded without being irradiated with ions 7, and the ions 7 are irradiated for sterilization treatment, and the results of the measurement of the microorganisms collected by the collector 6 can be compared. In addition, as for the measurement of the collected microorganisms, the elapsed time from the start of irradiation of the ions 7 or the elapsed time for the microorganisms to naturally decay can also be measured over time. In addition, by changing the intensity of the ions 7 of the irradiated microorganisms, and measuring the microorganisms used for each intensity of the ions 7, the sterilization treatment ability of the microorganisms in accordance with the intensity of the ions 7 can be evaluated. In addition, the above description is described as an example of ions 7 formed by irradiating positive and negative ions of particles for sterilizing microorganisms. In addition, for particles used for sterilizing microorganisms, pharmaceutical particles can also be used. When using pharmaceutical particles, the ion generating device 1 of the evaluation microorganism removal device 10 shown in FIG. 1 or the evaluation microorganism removal device shown in FIG. 2 is used. 21-(16) (16) 200403081 The ion generating device 1 at 20 2. The present invention can be implemented by changing to a means for spraying medicine particles. When the above-mentioned pharmaceutical particles are used, as the medicine, alcohol or aldehyde medicines, antiviral agents, insecticides, and the like can be used. <Third embodiment> Next, a third embodiment of the apparatus for evaluating microorganism removal according to the present invention will be described with reference to Fig. 8. Fig. 8 is a schematic configuration diagram showing a third embodiment of the apparatus for evaluating the removal of microorganisms. Compared with the second embodiment, a wind tunnel and other containers are provided outside the closed inner container. That is, as shown in the figure, the apparatus 30 for evaluating microorganism removal of this type is provided with a container 18, a microorganism injection tube 15 constituting a microorganism supply means, an ion generating element 12 constituting a microorganism removal means, and a microorganism adopting means. Its taking tube 13 and the microorganism taking device 6. Next, inside the container 18, a space from the injection pipe 15 to the collection pipe 13 is provided with a wind tunnel 31. Inside the wind tunnel 31, an ion generating element 12 is arranged. The internal space of the container 18 is a structure that blocks outside air, and adopts a form in which the size in the height direction is smaller than the size in the horizontal direction. On the single side wall of the container 18, a gasket 32 is inserted through the injection tube 15 from the outside into the container. In addition, on the side wall opposite to the injection tube 15, a tube 13 is taken through the gasket 3 2 To export to a container. The microorganism injection tube 15 is connected to the outside of the container 18 and is connected to the microorganism sprayer 11 and the microorganisms are fed by the microorganism sprayer 11. The microbial sprayer 11 sends microbial gas containing a certain concentration into the microbial injection pipe 15 at a certain speed. Secondly, the microbe sprayer 11 feeds -22- (17) (17) 200403081 The microbe-containing gas system of the microbe injection tube 15 is directed to the microbe injection port 15a in the container] 8 and injected into the container 18. In addition, at this time, the microbial monomer can be contained in the air and sent into the microbial injection pipe 15 or the solution of dispersed microorganisms can be misted, and the spray can be sent into the microbial injection pipe 15-the wind tunnel 3 1 in the container 1 8 to form a circle The tube is arranged approximately horizontally, and its two ends are arranged toward the injection pipe 15 and the taking pipe 13. The ion generating element 12 is disposed on the bottom surface on the slightly upstream side in the wind tunnel 31 outside the vertical downward region of the microorganism injection tube 15. The ion generating element 12 is an ion generating electrode arranged in a certain approximately flat shape, generating positive ions and negative ions, and sterilizing the microorganisms injected by the microorganism injection tube 15. The ion generating element 12 is the same as the ion generating element provided in the ion generating device 1 shown in FIG. 1, and the operation of generating the ion 7 is the same as the description of the ion generating device 1. The microbial collection tube 1 3 for collecting microorganisms is arranged horizontally on the opposite side of the microbial injection tube 15. One end of the collection tube 1 3 a forms a microbial collection port 1 3 a toward the inside of the container 18, and the other end is outside the container 18. , Is connected to the microbial collector 6. The microbial extractor 6 stores the foaming liquid inside, and the microorganisms sinking in the foaming liquid take the end portion of the tube 13 to foam the taken-in air and recover it. Secondly, the spray test systems of the container 18, the microbial sprayer 11 and the collector 6 are all covered by other containers 35. In addition, in this embodiment, the mist system -23 · (18) (18) 200403081 discharged from the injection port 15 a is sprayed into the inside of the wind tunnel 31, and there is some space between the injection port 15 a and the wind tunnel 31. In a small space, unused water drops fall on the container 18. In addition, the gas released from the injection port 15a has a certain speed depending on the spray, and at this speed, passes through the wind tunnel 31 at the direction shown by the arrow in Fig. 8. According to this mechanism, the particles formed by the ions emitted from the discharge electrodes of the ion generating element 12 react with the microorganisms, and when they reach the collector 6, the ion removal effect of the microorganisms can be confirmed. In addition, the method for evaluating microorganisms is not particularly limited, and all evaluation methods such as evaluation of agar medium, evaluation of cell culture, erythrocyte agglutination reaction, allergic reaction of organisms or cells, and microscopic observation can be used. In addition, in the mist containing microorganisms, the components that have not been vaporized and rapidly fall into a droplet shape or the components that have not been taken are accumulated in the wind tunnel 31. Although the container 18 contained therein has a structure that is difficult to leak out, However, because the outer side is covered with other containers 35, it is less likely to affect humans and the like located outside. Therefore, even if the wind tunnel 31 and the container 18 are not completely closed, the incidence of accidents such as biohazard can be greatly reduced. In addition, due to the shielding effect of the other container 35, unnecessary contamination can be prevented from being mixed in from the outside, and an effect of improving the accuracy of evaluation can be obtained. <Fourth Embodiment> Fig. 9 is a schematic diagram showing a device for evaluating the removal of planktonic microorganisms. In the evaluation device shown in the third embodiment, a particle discharge device is replaced by a needle discharge device 40. By. -24-(19) (19) 200403081 That is, this embodiment replaces the ion generating element 12 of the third embodiment, and replaces the needle discharge device 40. The needle-type discharge device 40 is located in the wind tunnel 31, and is composed of a needle-type discharge electrode 40a arranged on the upstream side region and an anti-plate electrode 40b arranged on the opposite side. Since the other configurations are the same as those of the third embodiment, the description is omitted. In this embodiment, when a positive or negative high voltage of about several kV is applied to the needle electrode 40a, a discharge is caused around the tip portion of the needle, and a gas mainly composed of positive or negative charged ions as ionic components is released. . According to the above composition ', a gas mainly composed of positive or negative ions is emitted, and the mist containing microorganisms emitted from the microbial injection tube 15 is irradiated to sterilize and remove microorganisms. Therefore, the microorganism removal evaluation test can be performed. In addition, in the present embodiment, the released particles 7 are not limited to ions. Free radicals, ozone, or other particles with bactericidal effects can be used. <Fifth Embodiment> Figure 10 shows the evaluation of plankton virus removal. The schematic diagram of the device. In this embodiment, in the evaluation device shown in the third embodiment, the particle emission portion formed by the ion generating element 12 is replaced by a radical emission mechanism of an ultraviolet lamp and a catalyst. That is, this embodiment mode replaces the ion generating element 12 of the third embodiment mode, and is provided with a radical emission mechanism 50. The free-radical emission mechanism 50 is located in the upstream side area in the wind tunnel 31, and is composed of an ultraviolet lamp 50a arranged at the center and a catalyst 50b arranged on the opposite side. Since -25- (20) (20) 200403081 is the same as the third embodiment, the description is omitted. The catalyst 5 Ob is composed of materials including platinum, gold, titanium oxide, and the like. 'Because of the radiation energy radiated by the ultraviolet lamp 50a, the energy is used to generate free radicals, which has the effect of releasing the generated free radicals into space. . In addition, the catalyst 50b is not limited to those containing materials such as platinum, gold, titanium oxide, and the like. Of course, as long as an active gas can be emitted, the same removal evaluation test can be realized. In addition, in this embodiment, the device for removing the catalyst 5 Ob can also be evaluated. At this time, the photons 7 of the fine particles emitted from the outside light 50a can sterilize the microorganisms existing in the space. In addition, the "lamp 50a" shows the radiation generated by this example. As the radiation, the light has an energy of 5eV to 20eV. "It has excellent sterilization performance. The radiation containing this light is applicable to this evaluation device, and the evaluation result obtained can be expected. In addition, as the above-mentioned radiation, an X-ray or r-ray-emitting element may be disposed at the position of the ultraviolet lamp 50a, and a test can be performed in the same manner. In addition, the 'appraisal method and evaluation device are effective methods of using the above-mentioned test of radiation. Of course, other particles can also be used, such as infrared rays and other hot rays and visible light, which can be evaluated according to the present invention. At this time, in principle, it can be considered that the sterilization performance is controlled by heat. When a strong hotline and visible light are required, it is desirable to provide a heat release mechanism or a light shielding plate. In addition, when using radiation, electromagnetic waves of 2 G Η z to 200 G 电磁 z can be sterilized. At this time, a catalyst is not necessarily required, and the constituent elements of microorganisms can absorb the energy of electromagnetic waves, and the microorganisms can be destroyed to a molecular level. because

-26- (21) (21) 200403081 As the required electromagnetic wave, for example, an electromagnetic wave of about 2.45 GHz which is easily absorbed by water can be used. For example, an electromagnetic wave of about 2 GHz to a short wavelength side can be used. In addition, in the frequency above 2GHz, it is considered that it is easy to be a candidate for electromagnetic waves in a higher frequency range absorbed by the constituent microbial elements such as proteins. Under the current situation of high-frequency components, as a possible frequency, 200 GHz may be considered as possible. Used for sterilization frequency. In addition, in the device shown in FIG. 10, an electromagnetic wave emitting element, an optical wave guide element such as an optical fiber, an electric heater, etc. may be provided at the position of the ultraviolet lamp 50a. <Target Microorganism> In addition, the sterilization target system to be performed in the present invention includes fungi, bacteria, viruses, and allergen-inducing substances (proteins, etc.). Secondly, in the practice of the present invention, monomers of these fungi, bacteria, viruses, and allergens can be used, or any combination thereof can be used. In addition, since the virus enters the range of general microorganisms, in the present invention, the effect of suppressing its proliferation is expressed by the words of sterilization or removal. In general, inactivating sentences are often used for the purpose of inhibiting the proliferation of viruses. Therefore, for viruses, in this specification, inactivating sentences can also be used instead of sterilizing or removing sentences. In the same way, regarding the allergen substance, in the present invention, the effect of suppressing the induction of an allergic reaction such as the human body is expressed by the words of sterilization or removal. Generally, the above effects can be replaced by inactivated sentences. In this specification, -27- (22) (22) 200403081 can also be used to replace the sentence of sterilization or removal of allergen substances. [Mode] Example [Example 1j] Example 1 'was implemented under the following conditions. As for the evaluation of the removal of microorganisms, an apparatus i 0 for evaluating the removal of microorganisms as shown in FIG. 1 was used. The container 8 of the apparatus 10 for evaluating the removal of microorganisms is one having a size of 2.0 m ', 2.5 m in width, and 2.7 m in height using an internal space. Secondly, the temperature of the ambient air inside the container 8 is 25 ° C, and the relative humidity is 42%. The internal space of the container 8 is stirred by the mixer 4. The stirring system with the mixer 4 was performed with an air volume of 4 m3 / min. Use of coliform bacteria as microorganisms. Regarding the supply of this coliform in the container 8, it is supplied to the microorganism injection port 5a in a mist form. Next, the coliforms are dispersed in the container 8 at a concentration of about 500 to 1,500 / m3. The collector 6 is configured using a Biotest Hyton RCS gas sampler. When collecting microorganisms with a gas sampler, the collection was performed at 40 L / min for 4 minutes. Next, the ions 7 formed by the positive ions and the negative ions generated by the ion generating device 1 are irradiated. In this Example 1, various ion concentrations are changed. The ion concentration is the number within a space of 10 cm from the ion sending part of the ion generating device 1 (ion generating port -28- (23) (23) 200403081 2). Next, after the coliforms are supplied into the container under the above-mentioned conditions, the ions 7 are irradiated at a certain ion concentration for one hour, and then the coliforms are collected by the above-mentioned gas sampler, and the number of the coliforms collected is measured. Next, the ion concentration of various ions 7 was changed, and the relevant measurement was repeated for each ion concentration. FIG. 3 shows the measurement results of Example 1. FIG. In Fig. 3, the horizontal axis corresponds to the ion concentration (number / cm3) of ion 7 in logarithm. In addition, in FIG. 3, the vertical axis system corresponds to the residual rate of plankton (%). The residual rate of phytoplankton is the percentage of the number of residual bacteria that have not been sterilized after irradiation with ion 7. As a result shown in FIG. 3, when the concentration of positive and negative ions emitted from the ion generating device i was increased, it was confirmed that the residual rate of airborne bacteria in the air was reduced. In addition, if the number of positive and negative ions is more than 10,000 / cm3, it is also confirmed that the residual rate decreases rapidly. Secondly, "the general indoor ion concentration is 500 to 1,500 pcs / cm3" as a general standard for effective microorganism removal effect, and it is considered that it is appropriate to send a positive ion concentration of more than 10 thousand pcs / cm. [Example 2;) Example 2 'was performed under the following conditions. As for the evaluation of the removal of microorganisms, the apparatus 10 for evaluating the removal of microorganisms shown in Fig. 1 was used. The container 8 of the apparatus 10 for evaluating the removal of microorganisms is the one having an internal space of 2.0 m in length and 2.5 in width and 2.7 in height. (24) (24) 200403081 Secondly, the temperature of the ambient air inside the container 8 is 251, and the relative humidity is 42%. The internal space of the container 8 is stirred by the mixer 4. The stirring system with the mixer 4 was performed with an air volume of 4rn3 / min.

J uses coliform as a microorganism. Regarding the supply of this coliform in the container 8, it is supplied to the microorganism injection port 5a in a mist form. Secondly, the coliform strain $ is dispersed in the container 8 at a concentration of about 1,000 pieces / m3. The collector 6 is configured using a B i 〇 t e s t H y t ο n R C S gas sampler. When collecting microorganisms with a gas sampler, the collection was performed at 40 L / φ per minute for 4 minutes. Next, the ions 7 irradiated to the ion generating device 1 are sent out, and the ions 7 not irradiated to the ion generating device 1 are not sent out to cause natural decay, and are collected by the above-mentioned gas sampler. When the ions were sent out, the ion concentration was within a space of 10 cm from the ion sending portion, and the positive and negative ions were 50,000 / cm. . Next, when the above-mentioned ions are being sent out and the ions are not being sent out, the above-mentioned gas sampler is used to collect coliforms every 15 minutes, and the number of bacteria of the collected coliforms is measured in spring. Fig. 4 shows the measurement results of Example 2 and shows the change with time of the residual rate (%) of the planktonic bacteria. In FIG. 4, the horizontal axis system corresponds to the elapsed time, and the vertical axis system corresponds to the phytoplankton residual rate (%) as in FIG. 3. When the ion is not sent out, the residual bacterial residue that naturally degenerates after 1 hour _ is 80%. On the other hand, when the ion was sent out, the residual rate of bacteria after 1 hour-10%. Regarding the above tests, -30- (25) (25) 200403081 is roughly a standard for judging the effect of removing microorganisms. If the accuracy of measuring microorganisms and the accuracy of concentration determination are considered, the residual rate of natural degradation is considered. When there is a difference of 10%, it is intentional difference. In addition, if the accuracy of the test is considered, the natural _

Test conditions A • ΛΓν, which is a decay rate of 50% or more after 1 hour, is suitable. FIG. 5 shows photographs of coliforms taken 15 minutes after the ion release and the non-ion release. FIG. 5 (a) is a person who performs ion emission, and FIG. 5 (b) is a person who does not perform ion emission. In addition, regarding the photography of the coliforms shown in FIG. 5, the coliforms taken in each of the above cases were cultured on an agar medium at 34 ° C and 100% RH for 48 hours, and then photographed. In addition, in FIG. 5, the size of the petri dish is 9 cm. When ion delivery was performed, as shown in Fig. 5 (a), no colonies of coliforms were observed. On the other hand, when ion delivery was not performed, as shown in FIG. 5 (b), colonies of coliform bacteria were observed. It can be seen from the results shown in FIG. 5 that sterilization is possible with ions. _ [Example 3] Example 3 was implemented under the following conditions. As for the evaluation of the removal of microorganisms, the apparatus 10 for evaluating the removal of microorganisms shown in Fig. 1 was used. The container 8 of the apparatus 10 for evaluating the removal of microorganisms is the one using internal space. The dimensions are 2.0 m in length, 2.5 m in width, and 2.7 m in height. Second, the temperature of the ambient air in the container 8 is 25 ° C, and the relative humidity is 42%. In addition, in the third embodiment, as described in the invention described below, stirring is performed in the container 8 -31-(26) · (26) · 200403081 Comparison of mixing and not stirring. The space in the stirring container 8 is a mixer 4 with an air volume of 4in3. / min. Stir. As a microorganism, Cladosporium, a fungus, was used. When the black mold is supplied into the container 8, the black mold is supplied from the microorganism injection port 5a in a mist state. Next, the concentration of black mold was dispersed in the container 8 at about 1,000 pieces / m3. The collector 6 is configured using a B i 〇 t e s t H y t ο n R C S gas sampler. When the microorganism is collected by a gas sampler, the collection is performed at 40 L / min for 4 minutes. Next, with the agitator 4 and without the agitator 4, the above-mentioned gas sampler was used to collect airborne bacteria in the air every 15 minutes, and the number of bacteria was measured. Fig. 6 shows the measurement results of Example 3, and shows the change with time of the residual rate (%) of planktonic fungi in the air that naturally decays with or without stirring. In FIG. 6, the horizontal axis system corresponds to the elapsed time, and the vertical axis system corresponds to the phytoplankton residual rate (%) as in FIG. 3. φ Without stirring, after 45 minutes, the residual rate of bacteria whose detection limit is 12%. On the other hand, during the stirring, the residual rate of bacteria was 80% because of natural decay after 1 hour. From the above results, the addition of stirring means can suppress the spontaneous fall of bacteria, and can easily evaluate the removal of planktonic microorganisms. In particular, when the mass of bacteria is large, _ stirring is effective. -[Example 4] -32- (27) (27) 200403081 The fourth example was implemented under the following conditions. As for the evaluation of the removal of microorganisms, the apparatus 10 for evaluating the removal of microorganisms shown in Fig. 1 was used. The device 8 for evaluating the removal of microorganisms is a container 8 having a size of 2.0 m in length, 2.5 m in width, and 2,7 m in height. Secondly, the temperature of the ambient air inside the container 8 is 25 ° C, and the relative humidity is 42%. The internal space of the container 8 is stirred by the mixer 4. The stirring system with the mixer 4 was performed with an air volume of 4 m3 / min. As a microorganism, Cladosporium, a fungus, was used. When supplying the black mold to the container 8, the microorganism injection port 5a is supplied in a mist form. Secondly, the concentration of black mold was dispersed in the container 8 at about 1,000 / m3. The collector 6 is configured using a Biotest Hy ton RCS gas sampler. When collecting microorganisms with a gas sampler, the collection was performed at 40 L / min for 4 minutes. Next, the ions 7 irradiated with the ion generating device 1 are sent out, and the ions 7 not irradiated with the ion generating device 1 are not sent out. The ions are naturally decayed, and are collected by the above-mentioned gas sampler. When the ions are sent out, the space between the ion concentration and the space 1 ocni of the ion sending section is 50,000 positive and negative ions, respectively. Next, when the ions were sent out and the ions were not sent out, black mold was collected every 15 minutes with the above-mentioned gas sampler, and the number of bacteria was measured. Fig. 7 shows the measurement results of Example 4 and shows the change with time of the residual rate (%) of planktonic bacteria. In FIG. 7, the horizontal axis corresponds to the elapsed time, and the vertical -33- (28) (28) 200403081 axis corresponds to the phytoplankton survival rate (%) similarly to FIG. 3. When the ion is not sent out, the residual rate of naturally decaying bacteria after 1 hour is 75%. On the other hand, when the ion was sent out, the residual bacteria rate after 10 hours had passed was 10%. Regarding the above measurement, as a general standard for judging whether the effect of removing microorganisms is effective, if the accuracy of measuring microorganisms and the accuracy of measuring the concentration are considered, a difference of 10% from the residual rate of natural decline is considered to be an intentional difference. In addition, if the accuracy of the test is taken into consideration, the natural decay when the ions are not sent out, and the test conditions for a bacteria residual rate of 50% or more after 1 hour are suitable. [Example 5] Example 5 was implemented under the following conditions. As for the evaluation of the removal of microorganisms, the apparatus 20 for evaluating the removal of microorganisms shown in Fig. 2 was used. The container 18 of the apparatus 20 for evaluating the removal of microorganisms was formed by using a quadrangular column having an inner space of 8 cm in size and 30 cm in length. Secondly, the temperature of the ambient air system in the container 8 is 25 ° C and the relative humidity is 50%. The polio treatment system uses polio lentivirus (p 〇 1 i 〇 v i 1 * us). Secondly, tens of thousands of polio virus aqueous solutions dispersed with CC were mixed with air into a mist form, and supplied at a rate of 0.1 cc / min and a wind speed of i.6 iri / sec to the container 18 through the injection port 15 a. Inside. In addition, 'irradiation of ions 7 to the above-mentioned virus by sterilization treatment is from the ion sending part of the ion generating element 12] space of 0 cm, so that positive -34- (29) (29) 200403081 negative ions are 100,000 / cm3. In addition, the poliomyelitis virus obtained after the sterilization treatment by irradiating the above-mentioned ions 7 was collected in the collector 6 by a solution foamer to capture the virus. Next, the poliomyelitis virus sterilized by irradiating the ion 7 was collected on the collector 6, and when the number of bacteria was measured, the virus removal rate was 78%. [Example 6] Example 6 was performed under the following conditions. FIG. 8 is a schematic structural diagram of a device for evaluating the removal of planktonic viruses in this embodiment, and FIG. 1 is a graph showing the frequency of cellular infection of influenza virus based on ion concentration, and FIG. 12 is a diagram of kerax virus based on ion concentration ((: OxsakU virus) cell infection frequency chart, Figure 13 is a graph showing the frequency of cell-based poliovirus infection. Figure 14 is a mass spectrum of positive and negative ions generated by ion generating elements. Figure 1 5 It is a flow chart of the evaluation test for comparison when the ion generating element is not operated and the ion generating element is operated. In this embodiment, as shown in the flowchart of FIG. 15, after making a solution containing microorganisms, the test device is used to spray The solution is taken in the space and its air is taken. In addition, after spraying, the sprayed air containing microorganisms is subjected to the steps of releasing particles having bactericidal effect and making them act. In addition, when the particles are emitted and not emitted. Test. The solution used in the above method, for example, the control method, erythrocyte agglutination reaction, etc., for microbial concentration Degree measurement or evaluation of the degree of activity, etc., to evaluate the effect of sterilization or inactivation 'by comparing the effect of the particles with and without the effect, we can confirm the effect of fixed particles. 35- (30) (30) 200403081 For the concentration of particles or the time of action of particles, the degree of sterilization or inactivation can be investigated in terms of irradiation time dependence or particle concentration dependence. This embodiment uses an apparatus 30 for evaluating the removal of microorganisms as shown in Fig. 8. Ion generating element 12 It is a flat surface discharge element with a length of 37mm and a width of 15mm. The positive and negative high voltages are applied between the electrodes, causing the surface of the surface electrode to discharge. One end of an acrylic cylindrical container 31 having an inner diameter of 55 mm and a length of 200 mm. One side of the above-built container 1 is equipped with a virus liquid sprayer 11 and the other side is equipped with a virus recovery device. 6. Influenza is inoculated into the allantoic membrane cavity of developing eggs, and cultured in an incubator, then the allantoic fluid is taken as the test virus solution. The 10 in 1 test The venom is discharged from a glass sprayer (viral sprayer 1 1) and connected to one end of the container 18. The other end of the container 18 is connected to a glass dust detector (taker 6) containing 10 ml of PBS (—). The sprayer The discharge pressure of the compressed air from the gas sampler is adjusted to 0.4 8 hPa with the gauge pressure, and the test virus is sprayed into the wind tunnel 31 in the container 18 from the injection port. The spray volume is 3.0 ml (spray flow rate lm) / minx Spray time 30 mi η) ○ At this time, the ion generating element 12 is controlled to be in a non-operating state, and the ion concentrations are compared with 200,000 / cm3, 100,000 / cm3, and 50,000 / cm3. -36- (31) (31) 200403081 The dust tester sucks the air in the test device at a suction flow rate of 10 L per minute for 30 minutes. The P B S (—) of the air heat in the capture test device attracted by the dust measuring device is a g-type test solution, which has passed the influenza virus, and is measured using a control method of main darby canine kidney (MDCK) cells. In addition, the Quesha virus and polio virus were measured by a control method against Hela cells. In addition, the so-called control method is to inject a virus-containing liquid into the cell in a way that confirms that the cell is infected with the virus. It can investigate the activity of the virus, that is, the frequency of virus infection or the ability of the virus to multiply in the cell. method. The ion concentration is from one side of the cylindrical wind tunnel 31 provided with the ion generating element 12 as described above, and a fan (not shown) is used to make the wind flow at a wind speed of 4m / sec, and the ion is at a distance from the other side. An air ion counter (product number 83-1001B) manufactured by DAN Scientific was set at 10 cm of the generating element, and the ion concentration in the space was measured. The space ambient temperature is 25t and the relative humidity is 60% R Η. The ion generation system was confirmed to have a temperature of 0 ° C and a relative humidity of 10% to a temperature of 40 ° C and a relative humidity of 90%. In addition, the above-mentioned blower is used for confirming the ion concentration. In the actual evaluation of microbial removal, the blower is not used inside the circular wind tunnel 31, and the wind generated by the spray of the sprayer 11 is set. As shown in Figure 11, when the frequency of cell infection of influenza virus when the ion generating element is not operating is 100%, 50,000, 10, and 200,000 (c / m: 3) ions occur. Significantly reduced the frequency of cell infection to 3.8%, 2.6%, and 0.5%. It was confirmed that increasing the ion concentration could improve the performance of removing influenza virus -37- (32) (32) 200403081. In addition, as shown in FIG. 12, when the frequency of cytotoxicity of the Quesacci virus when the ion generating element is not operated is 100%, when 50,000, 500,000 and 200,000 (pieces / cm3) ions occur, the Decrease the frequency of cell infection to 3.3%, 2.6%, and 1.1%. It was confirmed that increasing the ion concentration can improve the performance of removing the sakiz virus. In addition, as shown in Fig. 13, when the frequency of poliomyelitis virus cell infection when the ion generating element is not operated is 100%, when 50,000, 100,000, and 200,000 (pieces / cm3) ions are generated, the frequency of cell infection is greatly reduced. To 1.0%, 0.5% and 0.4%, it was confirmed that the performance of removing polio virus was improved due to the increase in ion concentration. The composition of the generated ions is shown in Fig. 14. The positive ions are plasma-discharged to ionize water molecules in the air to generate hydrogen ions Η +. The water molecules and hydrogen ions in the air are clustered due to the solvent and energy. In addition, the negative ion is a plasma discharge that ionizes oxygen molecules or water molecules' in the air to generate oxygen ions 02—clustering water molecules and oxygen ions in the air due to the solvent and energy. The positive and negative ions sent out of the space surround the virus floating in the air, and on the surface of the virus, the active source of hydrogen peroxide Η2 02 or free radicals · 0H is generated by the chemical reaction of positive and negative ions, which destroys the protein and kills it. According to this method, viruses in the air can be effectively sterilized. In addition, the method of investigating the activity level of the virus may be a method using a red blood cell agglutination reaction. A red blood cell agglutination reaction system injects a solution containing a virus into a solution containing chicken blood, for example, and observes the blood agglutination. When the virus is used, the presence of the erythrocyte agglutinin on the surface of the virus acts on most of the red blood cells, causing the agglutination of the red blood cells to confirm the existence of the virus. In addition, the method of investigating the virus concentration is to dilute the virus to various concentrations with an aqueous solution to confirm whether or not a red blood cell agglutination reaction has occurred. In contrast, the active virus concentration, that is, the virus concentration having the infectivity of active red blood cell agglutinin. [Example 7] FIG. 16 is a schematic diagram of an apparatus for evaluating the removal of planktonic pathogenic bacteria. Figure 17 is a graph showing the change over time of the concentration of Staphylococcus in the air at an ion concentration of 200,000 / cm3. The ion generator was the same as that used in Example 6. Fig. 18 is a graph showing the change over time of the concentration of staphylococcus in the air in the ion generating element and the ultraviolet-type ozone generating device. The space environment temperature is 25 ° C and the relative humidity is 60% PH. The ion generation system was confirmed to have a temperature of 0 ° C and a relative humidity of 10% to a temperature of 4CTC and a relative humidity of 90%. In order to detect the removal effect of the ion-generating element on Staphylococcus aureus existing in a certain space, this test uses the same basic structure as that shown in FIG. That is, the container 8 is an acrylic plate made of rain-resistant end of a 1 m X 1 m X 1 m FRP container using a space of 1 m 3. In this container, an ion generating element 1 is installed in the air blowing outlet above the air blower with an air volume of 2m3 / min. In addition, in order to float the sprayed bacteria for a long period of time, four 15 cm square axial fans (a X ia) -f 1 〇wfa η 4 were installed at the four corners of the container 8 with the wind direction -39- (34) (34 ) 200403081. One end of the acrylic plate portion of the container 8 was provided with an injection tube 5 for spraying bacterial liquid, and this was used as a test device. The test strains were inoculated and preserved in trypticase soy agar medium (BBL) and cultured at 35 ° C for 24 hours. This bacterium was diluted with sterilized physiological saline solution, adjusted and washed, and then used as a test bacterium. 10 ml of the test bacteria solution was put into a glass sprayer and connected to one end of the test device. The other end of the container 18 is connected to a glass dust tester containing 100 ml of a sterilized physiological saline solution. The discharge pressure of the compressed air from the gas sampler in the sprayer was adjusted to 0.48 hPa with the gauge pressure, and the spray test was performed from the spray port. The spray summer was 1 · 〇ιώ1 (spray flow rate 0.1ml / m i η X spray time 10 m i η). While spraying the bacterial solution, the axial fan 4 was operated until the end of the test, and continuous operation was performed. At the end of the spray, the air in the container 8 was sucked and collected for 10 minutes by a suction flow rate of 10 L per minute with a dust measuring device. Take this as 0 minutes. When the ion generating element 1 is operated, the ion generating element and the fan are operated simultaneously. After the start of the operation, after a certain period of time, 100 L of air in the trap container was sucked in the same manner as in the case of 0 minutes. The ion generation concentration is 200,000 / cm3. In addition, when the ion generating element 1 is not operated (natural decay), the ion generating element is not operated, and only the fan 4 is operated. At each time, it attracts and captures Air in container. In addition, in order to perform a comparison experiment with ozone, a UV-type ozone generator (0ZθΙΝ—1, SEN Special Light Source Co., Ltd.) was used to generate an ozone generation amount of -40-(35) the same as that of the ion generating element. (35) 200403081 1 · 6 3 7 gg / h (2 2 cC, 17% RH) for testing. The sterilized physiological salt solution of the air trapped in the container attracted by the dust tester was used as a test solution. The sterilized physiological salt solution was used to dilute it step by step. The original solution and each diluted solution were applied to tryptic casein soy agar medium (BB L). Incubate at 35 ° C for 48 hours. After incubation, the number of colonies on the medium was counted, indicating the number of bacteria that attracted air. As shown in Fig. 17, compared with the case where the ion generating element was not operated, after 30 minutes, it was confirmed that the concentration of plankton was reduced to about 1/10. Furthermore, no phytoplankton was detected after 60 minutes. As shown in Fig. 18, when the ion generating element was compared with the UV-type ozone generating device, it was confirmed that the concentration of plankton was reduced to about 1/10 after 60 minutes. Accordingly, the bactericidal effect of Staphylococcus, a representative bacterium of nosocomial infection, was confirmed in accordance with the effect described in Example 6.

[Example 8J FIG. 19 is a graph showing the change over time of the concentration of planktonic bacteria (B a c m s) in the air at an ion concentration of 200,000 / cm3. The same ion generating apparatus was used as in Example 6. Fig. 20 is a graph showing the change over time of the concentration of planktonic bacillus in the air of an ion generating element and an ultraviolet-type ozone generating device. In order to detect the removal effect of the ion-generating element on the planktonic bacillus existing in a certain space, in this test, an acrylic plate was installed at both ends of a FRP container of 1 π x lmxlm in a space of 1 m 3. Inside this container 'is the air blower outlet above the fan with an air volume of Sm3 / • 41-(36) (36) 200403081, with an ion generating element. In addition, in order to float the sprayed bacteria for a long period of time, four 15 cm square axial flow fans 4 are installed at the four corners of the container, and the wind direction is upward. One end of the acrylic plate portion of this container 8 is provided with an injection tube 15 for spraying bacterial liquid, and this is used as a test device. The test bacterial strains were inoculated with a medium for basal spore formation (Japanese Antibiotics Standard, June 30, 1987, Ministry of Health and Welfare Notice No. 117), and cultured at 35 ° C for 7 days. After the bacteria were washed with a sterilized physiological saline solution, it was heat-treated at 65 ° C for 30 minutes, and the formation of spores was confirmed under a microscope. The latter was washed with a sterile physiological saline solution and diluted with the latter, and used as spores. Put the test bacteria solution 1] into a glass sprayer and connect it to one end of the test device. The other end is connected to a glass-made dust side setter with 100 m] of sterilized physiological saline solution. The discharge pressure of the compressed air from the gas sampler in the sprayer was adjusted to 0.48 h Pa by the gauge pressure, and the test bacteria were sprayed from the spray port. The spraying amount was 1.0m1 (spraying flow rate 0.11 / 1/1) 11 &gt; &lt; spraying time 10min.). While spraying the bacterial solution, the axial flow fan 4 was operated until the end of the test, and the continuous operation was performed. When the spraying is finished, the air in the container is sucked and collected for 10 minutes by a suction flow of 10 L per minute with a dust measuring device. This is 0 minutes. When the ion generating element 1 is operated, the 'ion generating element operates simultaneously with the fan. After the start of the operation, after a certain period of time, 100 L of air in the trap container was sucked in the same manner as in the case of 0 minutes. The ion concentration was 200,000 / cm3. In addition, when the ion generating element 1 is not operated (natural decay) 値 -42-(37) (37) 200403081 The ion generating element is not operated, and only the fan 4 is operated. At each time, it attracts and collects Air in container. The space environment temperature is 25 ° C and the relative humidity is 60% PH. The ion generation system was confirmed to have a temperature of 0 ° C, a relative humidity of 10% to a temperature of 40%, and a range of relative humidity of 90%. In order to perform a comparison experiment with ozone, a UV-type ozone generator (OZ51N-1, SEN Special Light Source Co., Ltd.) was used to generate an ozone generation amount of 1.637 mg / h (22 °) which is the same as the ozone generation amount of the ion generating element. C, 17% RH). The sterilized physiological saline solution in the air trapped in the container attracted by the dust measuring device is a test solution, and the sterilized physiological saline solution is used for stage dilution. Incubate at 3 5 ° C for 4 8 hours. After incubation, the number of colonies on the medium was counted, indicating the number of bacteria that attracted air. As shown in Fig. 19, it was confirmed that the concentration of phytoplankton was reduced to about 1 / 10th after 30 minutes compared with the case where the ion generating element was not operated. Furthermore, no phytoplankton was detected after 120 minutes. As shown in Fig. 20, when the 'ion generating element was compared with the ultraviolet-type ozone generating device, it was confirmed that the concentration of plankton was reduced by about half after 60 minutes. Accordingly, the bactericidal effect of Bacillus spp. Which formed heat-resistant spores was confirmed by the action described in Example 6. Anthrax and Bacillus are the same genus, and their effects can be expected. [Embodiment 9] -43-(38) (38) 200403081 Fig. 21 is a cross-sectional view showing an air-conditioning apparatus disposed in the air outlet of the ion generating element. Fig. 22 is a graph showing the frequency of cellular infection of plankton virus in the air after 60 minutes with respect to the ion ejection amount in a space of 27 L. Fig. 23 is a graph showing changes over time in the frequency of virus cell infection in the air when a positive and negative ion of 5400,000 / cm3 is supplied in a space of 30 m3 in volume per minute. In the test shown in Figure 22, in order to detect the effect of ion ejection on the frequency of cell infection of plankton influenza virus in the space, the 27L space used in this test was 30cmx30cmx30cm. A virus spray device and a recovery device are installed at both ends of the chlorinated olefin container. In this container, an ion generating element is mounted on the air blowing port portion 'above the blower fan. In addition, in order to achieve the purpose of floating the sprayed virus for a long time, the axial direction of the axial flow fan is set upward. Influenza virus A (HINI) A / PR8 / 34: ATCC VR-95 was inoculated into the allantoic membrane cavity of developing eggs, cultured in an incubator, and allantoic fluid was taken as a test virus fluid. Put the test virus solution of 10 m 1 into a glass sprayer and connect it to one end of the test device. The other end is connected to a glass dust tester that puts 100ml of sterilized physiological salt solution. The compression from the gas sampler in the sprayer The discharge pressure of the air is adjusted to 0.48hPa by the gauge pressure, and the test bacteria are sprayed from the spray port. The spray amount is 3.0 ml (spray flow rate (him) / min X spray time 30n] in). While spraying the virus solution The axial flow fan 4 is operated until the end of the test, and the continuous operation is performed. When the spraying is finished, the air in the container is dust-measured with a suction flow of -44-(39) (39) 200403081 for 10 minutes per minute. Suction and capture for 30 minutes. This is 0 minutes. One hour after the start of operation, 100 L of air in the trap container was sucked in the same manner as 0 minutes. PBS sucked by the dust tester | PBS (- ) Is the test solution, influenza The virus was measured using the control method of MDCK cells. The input voltage of the ion generating element was adjusted to adjust the ion ejection amount. The positive and negative ion ejection amounts were 0 / cm3. When the cell infection frequency was 100%, 270,000 were confirmed. The frequency of cell infections with an ion ejection amount of more than / cm3 · min has been rapidly reduced, and it has been confirmed that the positive and negative ion ejection amounts of 270,000 / cm3 or more have the effect of reducing the virus infection ability. Furthermore, in order to confirm that it is actually used in residence The effect of the environmental space is shown in the test shown in FIG. 23. In a space volume of 30 m3, an air conditioning device 60 as shown in FIG. 21 is installed, and the state of the dust collection filter 64b and the deodorizing filter 64a is removed, so that the ion Residual rate of plankton virus in the space when the generating element 65 is operating. Here, when the structure of the air-conditioning apparatus 60 shown in FIG. 21 is described, the air-conditioning apparatus 60 forms an air intake port in front of the indoor apparatus 6 62. An air outlet 63 is formed above the device 61. The air inlet 62 is provided with a deodorizing filter 64a and a collection filter 64b. An ion generating device 67 formed by the ion generating element 65 and the high-voltage power source 66 is provided. Second, the air fan 68 inside the device draws the air sucked in by the air suction port 62 through the air blowing port 63 to the outside. At this time, Driven by the ion generating device 67, the ionized air -45-(40) (40) 200403081 is released. The air conditioning device configured as described above is provided with an ion generating element 65 on the blowing air path, and the air sucked in by the suction port 62. When discharged from the air outlet 63, the air is allowed to contain ions, and the ions can be released into the space. Therefore, 'is not only the ions added to the inhaled air, but ions can be added to the entire space. The virus concentration measurement was performed in the same manner as in the test performed in FIG. 22. 5.4 million ions / cm3 of positive and negative ions were supplied in one minute. Understand that the frequency of cellular infections per hour is reduced to 1/10. In this way, regarding the volume of an air-conditioning apparatus actually used in a living environment, it is understood that the effect of virus inactivation in the air can be evaluated. The positive and negative ions sent out of the space surround the virus floating in the air. On the surface of the virus, the hydrogen peroxide H2O2 or the free radical · OH 'which is the active source due to the chemical reaction of the positive and negative ions destroys the protein and kills it. According to this method, viruses in the air can be effectively sterilized. In this embodiment, the ion system indicates both positive and negative ions. Regarding the ion concentration, each ion concentration is approximately equal, and the average radon is recorded. In addition, in all of the above embodiments, as the particle release method, the method using the Lenar effect can be used, even if the liquid generates ejection or vibration, using physical separation and the generation of particles with electric charges. The effect of the present invention is obtained. In addition, as the particles, positive ion, negative ion, mixed gas of positive and negative ions, charged particles such as α-rays and / 3 rays other than the above, various kinds of ionized gas particles, free radical particles, and pharmaceutical particles may be used. The same effects as the present invention are obtained. -46-(41) (41) 200403081 The effect of the invention is as explained above. According to the present invention, the microorganisms are floated in a certain space. For the microorganisms, the particles used for sterilizing the microorganisms are irradiated with ions and the like. Microorganisms are measured to achieve the effect of measuring the bactericidal treatment ability of the particles on the microorganisms. [Brief description of the drawings] Fig. 1 is a schematic configuration diagram showing the first embodiment of the apparatus for evaluating microorganism removal according to the present invention. Fig. 2 is a schematic configuration diagram showing a second embodiment of the apparatus for evaluating microorganism removal according to the present invention. Fig. 3 shows the measurement results of Example 1, and the measurement results of the microorganisms used in the sterilization treatment when the ion concentration was changed. Fig. 4 shows the measurement results of Example 2 regarding the results of measurement of microorganisms taken with and without ions being sent. Fig. 5 is a photograph obtained by photographing the microorganisms taken in Example 2. Fig. 5 (a) is a photograph of the microorganisms taken when the ion is sent out, and Fig. 5 (b) is a photograph of the microorganisms taken when the ion is not taken out. Fig. 6 shows the measurement results of Example 3, the measurement results of the microorganisms taken in the stirred container and when not stirred. -Fig. 7 shows the measurement results of Example 4 regarding the measurement results of the microorganisms taken with and without the ions being sent. -47- (42) (42) 200403081 Fig. 8 is a schematic diagram showing a third embodiment and a sixth embodiment of an apparatus for evaluating microorganism removal. Fig. 9 is a schematic configuration diagram showing a fourth embodiment of the apparatus for evaluating the removal of planktonic microorganisms. FIG. 10 is a schematic configuration diagram showing a fifth embodiment of the apparatus for evaluating the removal of plankton virus. FIG. 11 is a graph showing the frequency of cell infection of influenza virus according to the ion concentration of Example 6. FIG. FIG. 12 is a graph showing the frequency of cell infection of the Quexach virus according to the ion concentration of Example 6. FIG. Fig. 13 is a graph showing the frequency of cell infection of polio virus according to the ion concentration of Example 6. FIG. 14 is a mass spectrum chart showing positive ions and negative ions generated by the ion generating element of Example 6. FIG. FIG. 15 is a flowchart showing an evaluation test in Example 6. FIG. FIG. 16 is a schematic diagram of an apparatus for evaluating the removal of planktonic pathogenic bacteria in Example 7. FIG. Fig. 17 is a graph showing the change over time of the concentration of Staphylococcus in the air at an ion concentration of 200,000 particles / cm3 in Example 7. Fig. 18 is a graph showing the change over time of the concentration of staphylococcus in the air in the ion generating element and the ultraviolet-type ozone generating device of Example 7; FIG. 19 is a graph showing the change over time of the concentration of Bacillus bacillus (Bacil) us in the air at an ion concentration of 200,000 cells / cm3 in Example 8. -48-(43) (43) 200403081 Fig. 20 is a graph showing the change over time of the concentration of planktonic bacteria in the air in the ion generating element and the ultraviolet-type ozone generating device of Example 8. FIG. 21 is a cross-sectional view showing an air-conditioning apparatus provided in an air path of an ion generating element blowout port in Example 9. FIG. Fig. 22 is a graph showing the frequency of cell infection of plankton virus in air according to the amount of ion ejection in Example 9. Fig. 23 is a graph showing changes over time in the frequency of infection of virus cells in the air according to the amount of ion ejection in Example 9. Reference symbols 1 Ion generator 2 Ion generator □ 3 Microbial collection tube 3a Microbial collection □ 4 Mixer 5 Microbial injection tube 5a Microbial injection □ 6 Microbial collector 7 ψ Pine 8 Container 10 Evaluation device for microorganism removal 11 Microbial sprayer 12 Ion Generating element 12a Ion-generating electrode-49 · (44) '(44)' 200403081 13 Microbial collection tube 13a Microbial collection port 15 Microbial injection tube β 15a Microbial injection port 18 Container 20 Device for evaluating microorganism removal 3 1 Wind tunnel 35 Container #

-50-

Claims (1)

200403081 ⑴ Pickup, patent application scope 1 · A method for evaluating the removal of microorganisms, which is characterized by supplying microorganisms to the internal space of the container and irradiating particles formed by positive and negative ions used for sterilizing the microorganisms. After the particles are irradiated, Microorganisms were taken and the measurement of the microorganisms taken was performed. 2 · A method for evaluating the removal of microorganisms, characterized in that a virus as a microorganism is supplied to the inner space of the container, and particles used for sterilizing the microorganism are irradiated. . 3. If the method of evaluating microorganism removal in item 1 or 2 of the scope of the patent application 'wherein the particle is irradiated, the measurement of the microorganism is performed at the same time' and further, the same conditions as when irradiating the particle to sterilize the microorganism Next, the microorganisms are supplied, and the particles are not irradiated. After the microorganisms naturally decay, the microorganisms are taken, and the measured microorganisms are measured. 4. The method for assessing the removal of microorganisms according to item i or item 2 of the scope of the patent application, wherein the determination is a determination of the concentration of the microorganism or a determination of the activity. 5. The method for evaluating microorganism removal according to item 1 or 2 of the scope of the patent application, wherein the measurement of the microorganisms used is to further measure the change over time according to the time of irradiation of the particle. 6. If the method for evaluating microorganism removal is described in item 1 or 2 of the scope of the patent application, the determination of the microorganisms used is to further determine the concentration dependence of the removal performance of the particles. 7 · If the method for evaluating microorganisms in item 1 or 2 of the scope of patent application is -51-(2) (2) 200403081, the method for supplying microorganisms to the internal space of the container is to fog the solution of dispersed microorganisms And spray. 8 · If the method for removing microorganisms is evaluated in item 1 or 2 of the scope of the patent application, wherein the microorganisms are evaluated using cell culture of microorganisms, erythrocyte agglutination reaction of microorganisms, or allergic reaction of microorganisms. 9 · If the method of evaluating microorganism removal according to item 2 of the patent application 'in which the particles used for sterilizing the microorganisms are discharged from any kind of air', particles generated by irradiation with radiation in the air and Lenardeffect . 10. The method for evaluating the removal of microorganisms according to item 2 of the scope of the patent application, wherein the particles used for sterilizing the microorganisms are any of radiation, X-rays, r-rays, and electromagnetic waves. 11. The method for evaluating the removal of microorganisms according to item 2 of the scope of the patent application, wherein the particles for sterilizing the microorganisms are pharmaceutical particles. 1 2 · The method for evaluating the removal of microorganisms according to item 1 of the scope of the patent application, wherein the microorganism is one or a combination of two or more selected from the group consisting of bacteria, fungi, viruses, and allergens. 13. As for the method for evaluating microorganism removal in item 1 or 2 of the scope of the patent application, regarding the space inside the container, when supplying microorganisms, for the microorganisms supplied in the container, the internal space of the container is stirred from the lower side. . 14. · A device for evaluating the removal of microorganisms, comprising an internal space capable of supplying microorganisms and a sterilizing treatment for the microorganisms -52- (3) (3) 200403081 container, and a space for supplying microorganisms to the container Microbial supply means' and a microorganism removal means for simultaneously supplying particles formed by positive and negative ions for sterilizing the microorganisms in the space inside the container, and after the microorganisms are sterilized by the microorganisms removing means, the microorganisms of the microorganisms are taken Measures are taken to evaluate the microorganisms taken by the microorganisms. 15 · An apparatus for evaluating the removal of microorganisms, comprising an internal space capable of supplying a virus as a microorganism, a container for sterilizing the microorganism, and a microorganism supply means for supplying the microorganism to the internal space of the container, and The container internal space is provided with a microorganism removing means for sterilizing the microorganism particles, and after the microorganism removing means is used for the sterilizing treatment of the microorganism, the microorganism adopting the microorganism adopting means is used to measure the microorganisms adopting the microorganism adopting means for evaluation. . 16 · If the device for evaluating microorganism removal in item i4 or item 15 of the scope of patent application, the microorganism supply means, the microorganism removal means, and the microorganism adoption means are located in the air path containing the microorganisms, from the upper side toward the On the downstream side, they are arranged in order. 1 7 · If the assessment microorganisms in item i 4 or item 5 of the scope of application for patents are based on the microorganisms @S 2 device, where the microorganism supply means and the microorganisms adopt means, there is a wind tunnel forming an air path containing microorganisms, the wind tunnel The microorganism removal means is arranged inside. 18 · If the device for assessing microorganism removal in item 14 or item 15 of the scope of the patent application, the microorganism removal means and the microorganism taking means are arranged in a region other than vertically below the microorganism supply means. -53- (4) (4) 200403081 1 9 · If the device for evaluating microorganism removal in item i4 or item 15 of the scope of patent application, the other side of the container is equipped with another container that can cover the container. 20. The device for assessing microorganism removal, such as the scope of application for item No. 14 or No. 15, wherein the inner space of the container is provided with a stirring means for stirring the inner space. 2 1 · If the device for evaluating microorganism removal in item i4 or item 15 of the scope of patent application, the microorganism supply by the microorganism supply means is to make the solution of dispersed microorganisms into a mist, and spray the solution in the internal space of the container Make up. 22. The device for evaluating microorganism removal, such as the scope of application for patent item No. 14 or No. 15, wherein the particles used for sterilizing the microorganisms are discharged from any kind of air, irradiated with radiation in the air, and the Lenard effect effect). 23. The device for evaluating microorganism removal according to item 15 of the scope of the patent application, wherein the microorganisms are sterilized by radiation, X-rays, 7-rays or electromagnetic waves. 24. The apparatus for evaluating microorganism removal according to item 15 of the scope of the patent application, wherein the microorganism removal means is constituted by irradiating pharmaceutical particles as germicidal particles for the microorganisms. -54-