TWI232112B - Process of preparing porcine dressing - Google Patents

Abstract

This invention related to a process of preparing dressing from healthy adult porcine skin. Firstly, raw porcine hide has to be cleaned and subdermal fat tissue has to be removed. Then, the hairs and hair roots were cut and scraped completely. The clean surface was treated with heptane, octane or nonane, and disinfectant before skin grafting. Then, the skin-grafts were put into a sterilizing solution composing of sodium hypochlorite or hydrogen peroxide and irradiated with Co-60. This is a simple, effective and low-cost process that can be produce a porcine dressing for further fabrication into medical devices.

Description

1232112 _Case No. 881Π733_ Year and month revision_ V. Description of the invention (1) (1), development background: Pigskin has been used as a biological dressing for more than 20 years. Generally, pigskin dressings are considered to heal wounds With positive help, it can reduce the loss of water, electrolytes and proteins, reduce the pain of patients' wounds, and the pigskin dressing with a complete skin structure can prevent the invasion of foreign bacteria. The United States is the earliest country to have such products, such as pig skin dressings produced by the company, and Japanese companies have similar products, such as dry pig skin dressings made by Dapeng Co., Ltd. However, these products have hair root residues, which results in poor adhesion of the dressing, and it is easy to cause sticking to the wound, and can irritate the patient's wound and cause pain. There are two sources of domestic pig skin dressings for domestic medicine: Scald Center) make and rely on imported products. Domestic handmade pigskin dressings are cumbersome and of varying quality and cannot be mass produced. Foreign products are expensive, and they were imported urgently in the event of major casualties, such as the burns and burns of Golden Gate soldiers. Regarding medicinal pigskin dressing, it has been used abroad for several years. The domestic Taiwan Pig Research Institute also proposed an invention patent on pig skin dressing in the Republic of China in 1975 (Announcement No. 1 1 5 9 7 2), and China also has related patents (CN 1 0 6 0 6 1 1 A ). As for the patent filed by the Taiwan Pig Research Institute in 1975, the dressing made by this patent method can maintain the basic structure of pigskin (complete cuticle, epithelium and dermis) and enter clinical experimental research. (41st General Scientific Meeting, Surgical Association, Repub 1 icof C hina), but the finished product will still have hairy roots, and it is not easy to mass produce and supply patients. Patents in mainland China,

1232112 _Case No. 881Π733_ 年月 日 __ V. Description of the invention (2) The chemical method (aqueous solution of sodium sulfide and calcium hydroxide) is used to remove hair roots from pig skin, strengthen the hardness of pig skin, and increase the skin This method is simple to make, but treating pigskin with sodium sulfide solution requires extremely high pH (pH > 11). The removed pigskin needs a lot of water to wash to reduce p Η value, and the sodium sulfide solution is a strong oxidant. Will destroy the original structure and upper epidermal layer of pigskin collagen, reduce the effectiveness of pigskin dressings against the invasion of foreign bacteria. In view of this, we have invented an improved manufacturing method of fresh pigskin dressings, which is low cost and the resulting The finished dressing is free of medicaments and hair roots, and can retain a complete full cortex structure. This method is beneficial to the mass production of dressing products in China, so as to facilitate the use of people in the country of supply.

C) Detailed description of the invention: 1. Description of the method steps of the invention (1), pre-treatment (a) Take pig skins that are fresh and normal in appearance, without skin diseases and trauma, and clean and remove subcutaneous fat and muscle tissue. Fix it on the leather bed and cut off the long hair with electric scissors. (b) Scrub the surface of the raw pork skin with heptane, octane, or octane, and then shave the hair off with a razor blade.

(c) Spray the liquid bacteriostatic agent containing iodine ions or silver ions or quaternary ammonium uniformly on the surface of pig skin, and then scrape off the remaining hair roots on the surface. (d) Spray 75% alcohol-soluble water on the surface of pigskin before taking the skin

Page 6 1232112 _Case No. 881Π733_ Year Month Date__ V. Description of the invention (3) Liquid, after drying, spray with sterilized liquid paraffin. (2) Take the skin (a) Adjust the scale of the skin picker (Z i ra mer) to an appropriate thickness (8/1 Ο Ο 0 ～ 3 Ο / 1 Ο Ο 0 inch) and width (2 to 4 British pair), to take the skin. (b) Remove the pigskin, rinse it with sterile water until it is bloodless, and soak it in a solution containing (Κ Ο 1〜1% wt sodium hypochlorite (NaCIO) or 0.1% ~ 5% wt hydrogen peroxide (H202) 2 0 ~ 120 minutes. Rinse with sterile water until the pigskin has no odor.

(3) Packaging The pig skin dressing was immersed in 15% glycerol for 2 to 6 hours. The pigskin dressing was placed on the sterile gauze with the mesh facing down, rolled up flatly, placed in a sterilized centrifuge tube, sealed, and irradiated with cobalt 60 rays (2 5 KG y, 3 to 5 hours) for sterilization. Store in a refrigerator at -20 to -30 ° C. 2. The biological dressing prepared by the method of the present invention has the following advantages:

(1) The pig skin dressing prepared by the method of the present invention has a complete structural cortex, including a complete cuticle, epithelium and dermis, which can reduce the loss of water, electrolytes and proteins, and prevent the invasion of foreign bacteria. (2) The pigskin dressing prepared by the method of the present invention does not leave hair roots, reduces the phenomenon of wound adhesion, and reduces the pain of patients. (3) The method of the present invention uses only a small amount of medicine and a large amount of sterile water

Page 7 1232112 _ Case No. 88 Π 7733_ Year Month Amendment _ V. Description of the invention (4) Rinse, the finished product has almost no drug residue. (4) The biological dressing made by the present invention has no damage to the natural structure and can maintain good adhesion. (5) The method of the present invention has a simple process and can produce pigskin dressings of different thicknesses depending on the medical conditions. (6) The present invention belongs to a method for manufacturing a temporary biological dressing in medical equipment. The finished product can be used for burns, burns, and wound dressing and covering. (3) Detailed description of the diagram * The first picture is a slice of the whole cortex tissue structure of raw pig skin. Pig skin with normal fresh appearance, without skin disease and trauma, was stained with Η & E after tissue sectioning. This picture shows the complete structure of the whole cortex of pig skin when the tissue section is enlarged by 20 ×, A: cuticle; B: epithelial layer; C: dermal layer; I): fat layer; E: hair follicle tissue. The second figure is a slice of the whole cortex tissue structure of 0.01 6 inch thick pigskin dressing. The fresh pig skin dressing obtained by the manufacturing method of the present invention is subjected to H & E staining after tissue sectioning. This picture shows that when the tissue section is enlarged at 40X, it can be clearly seen that the pigskin dressing still retains the complete full cortex structure, A: cuticle; B. epithelium; C: dermis. The third figure is a section of the whole cortex tissue structure of a pig skin dressing with a thickness of 0.030 inches. In the case where the fresh pigskin dressing obtained by the manufacturing method of the present invention is subjected to tissue slicing, Η & E staining is performed. This picture shows that when the tissue section is enlarged by 40X, it can be clearly seen that the pigskin dressing still retains the complete full cortex structure, A: cuticle;

1232112 _ Case No. 88117733_ Year Month Amendment _ V. Description of the invention (5) B: epithelial layer; C: dermal layer. (IV) Example: Firstly, the fresh pig skin raw material 5 with normal appearance, no skin disease and trauma, and its tissue structure is shown in the section diagram of Fig. 1. After cleaning, remove excess fat and muscle tissue, fix it on the leather bed, and cut off long hair with electric scissors. Brush the surface of pigskin with straight chain octane (C8H18), after dissolving the grease on the surface of pigskin, the roots will be erected, and then the hairs on the skin will be shaved off with a razor in the direction opposite to the hair. Then spray P o v i d o n e I o o d i n e on the pigskin, scrape the hair roots on the surface in the direction of reverse hair and along the hair. Spray the 75% alcoholic alcohol solution before taking the skin. After drying, spray the sterilized liquid paraffin. Adjust the scale of the peeling machine to 0.016 inches and 4 inches wide for peeling. Remove the pigskin and rinse it with sterile water until it is bloodless. Soak in 0.05% by weight sodium hypochlorite (NaC 10) aqueous solution for 30 minutes to remove the fishy smell of pigskin, and then rinse with sterile water until the pigskin has no odor. Figure 2 and Figure 3 are pig skins obtained according to the present invention The tissue section of the dressing can be seen from the section and its structure is very complete. Soak the pigskin dressing in 15% glycerol for 2-6 hours. The pigskin dressing was placed on the sterile gauze with the mesh facing down, rolled up flatly, placed in a sterilized 50 n] L centrifuge tube, sealed, and irradiated with cobalt 60 rays (25 KGy, 3 ~ 5 hours) . The finished product is stored in a refrigerator at -20 to -30 ° C. The pig skin dressing prepared by the present invention does not have any residual medicine and hair roots, and has a complete skin layer with a complete structure, which is suitable for burn, burn, and wound wound covering and dressing.

1232112 Case No. 88117733 _ Month Revision Amendment of the drawing briefly The first picture, the complete structure of the entire cortex of pigskin. The second picture, the detailed structure of the whole skin layer of a pig skin dressing of 0.016 inches The third picture, the detailed structure of the whole skin layer of a pig skin dressing of 0.016 inches

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Claims (1)

1232112 / -l · ^!, Gu Shen 丨 Case No. 88Π773ί Scope of patent application A method for manufacturing a fresh pig skin dressing, comprising the following steps: (1) taking fresh pig skin with normal appearance, without skin disease and trauma, and washing it to remove fat, moon II meat tissue and hair; (2) geng After removing the grease on the surface of pigskin with alkane, octane or nonane, shave the razor blades of upright hair, and then spray the bactericide on the surface of pig skin, and then remove the remaining hair root with a blade; (3) After the ulcer is sterilized with alcohol, it is rented with liquid stone trowel to remove the surface layer of pig skin with the required volume and thickness using a skin machine, and rinsed with g-free water to blood-free water; (4) Soak the removed pig skin in (KOI ~ 1% wt sodium hypochlorite (NaCIO) aqueous solution or 0,1¾ ~ 5% wt hydrogen peroxide (fi2 02) for 20 ~ 120 minutes, then rinse with sterile water until the pig skin is free. Peculiar smell; (5) the obtained pigskin is obliquely packaged and then irradiated with cobalt 60 rays to eliminate g. The manufacturing method such as the scope of the patent application] item, wherein the proposed agent may be an iodine-containing bacteriostatic agent, Liquid bactericidal preparation containing silver ion inhibitor or western ammonium ... '' A pigskin dressing made by the first method of patent application, which has no hairy roots and remains whole Cortical complete structure and sterile contaminated pigskin dressing. Page 11