TW470749B - Propolis extract with improved water-solubility - Google Patents

Propolis extract with improved water-solubility Download PDF

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TW470749B
TW470749B TW86103834A TW86103834A TW470749B TW 470749 B TW470749 B TW 470749B TW 86103834 A TW86103834 A TW 86103834A TW 86103834 A TW86103834 A TW 86103834A TW 470749 B TW470749 B TW 470749B
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propolis
weight
parts
extract
propolis extract
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TW86103834A
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Chinese (zh)
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Hajime Aga
Takashi Shibuya
Shoichi Hamada
Satoshi Iritani
Toshio Miyake
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Hayashibara Biochem Lab
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Abstract

Disclosed are a propolis extract which is prepared by adjusting the pH of propolis to 5.5-7.0 to improve the water-solubility of the effective ingredients of propolis, a composition containing the propolis extract, and a method for improving the water-solubility of propolis extracts.

Description

470749 A7 一 B7 五、發明説明（1 ) 本發明之背景 本發明之領域 本發明係關於水溶性改良的蜂膠萃取物，詳而言之， 本案係關於蜂膠有效成份之水溶性藉由將蜂膠萃取物之 pH調爲5. 5 — 7而獲改良的蜂膠萃取物，其製備及其 •用途，還關於供改良蜂膠萃取物之水溶性的方法。 習知技藝之說明 如Propolis in Natural Therapeutics ( 1 9 8 3 )，二次修正版（Librairie Maloine S. A. Editeur， Paris， France出版）、Fragrance Journal， No. (請先閱讀背面之注意事項再填寫本頁) 8 3， ρρ. 2 0 — 2 8 及pp. 3 6 -4 3 (1 9 8 7 及 Ap i do logie， Vol. 2 2 ， pp . 15 5 — 16 2 ( ，νβ 1 9 9 1 )所述者，蜂膠爲樹脂狀的產物，其係由蜜蜂儲 存於蜂窩中，含有樹脂、蜂蠟、精油、花粉及黃酮類化合 物，且長久以來已被用於各種民間用藥。 經濟部中央標準局員工消费合作社印製 蜂膠之主要活性爲防腐活性、抗氧化活性、抗發炎活 性、局部麻木性、抑制病毒生長的活性、免疫控制活性及 巨噬細胞活化活性，乃係已知者，且蜂膠之主要成份亦已 知爲黃酮類化合物、芳族羧酸類化合物、及芳族醛類化合 物。 在本發明之前，本發明之發明人已對於蜂膠之有效成 份進行研究。結果發現了 一有效成份，3 — [4 _羥基— 3 ，5 —二（3 —甲基—2 —丁烯基）苯基]-2 —丙烯 本紙張尺度適用中國國家標準（CNS ) Α4規格（210 X 297公釐）-4 - 470749 Α7 Β7 五、發明説明（2 ) 酸，別名爲「 Ar tep i 1 1 i n C」（在下文中稱爲「物質C」 )，其呈現出防腐活性、毛髮生長活性及抗腫瘤活性，且 因而完成與物質C之用途有關之發明：例如，日本專利公 開第256 ，177/94號揭示含有物質c爲有效成份 之防腐劑，日本專利公開第3 1 2 ’ 9 1 8 / 9 5號揭示 含有物質C爲有效成份之毛髮恢復劑，而日本專利公開第 1 9 1 ，0 1 5 / 9 5號則揭示含有物質C爲有效成份之 抗腫瘤劑。 如前所述，本案之發明人已證實物質C乃蜂膠中的重 要物質之一。 由於蜂膠係黑棕色的塊狀產物且由於其主要係由不溶 於水之疏水性產物所組成，因此無法輕易地使用，而通常 係以液態蜂膠萃取物的形式或是蜂膠氣味的形式使用，彼 等形式係藉由將蜂膠溶解於高濃度之易溶於水有機溶劑的 溶液中，且用該溶液予以萃取所得，該溶劑可爲，例如， 丙酮、乙酸及低級醇類，諸如，甲醇、乙醇、異丙醇等。 一般而言，以乙醇最佳。 經濟部中央標隼局負工消費.合作杜印製 (請先閲讀背面之注意事項再填寫本頁) 然而如是之萃取物在用於食品（諸如健康食品）、抗 易感受性疾病之藥劑（諸如易感受性疾病之治療及/或預 防劑）、以及化粧品（諸如，皮膚細緻劑及皮膚增白劑） 時，具有下列之缺點，因而使得蜂膠在應用於各領域時有 所困難。 (1 )蜂膠萃取物之成份（諸如，樹脂及蠟）易於結晶化 。在用唾液稀釋蜂膠萃取物時，其成份易於結晶化 本紙张尺度適用中國國家標準（CNS ) Α4規格（2丨Ο X 297公釐）-5 - 470749 A7 B7 五、發明説明（3) ，這會強烈抑制彼等成份爲活體所吸收及利用。當 用水稀釋蜂膠萃取物時，蜂膠之成份會大量結晶化 ，使得處理變得非常困難； (2 )蜂膠萃取物之顔色未能令人滿意且萃取物易於變爲 深棕色或不透明；以及 (3 )蜂膠萃取物之氣味及味道難令人滿意，亦即，其具 有泥土味且有刺激或刺鼻的味道。 爲了克服此等習知蜂膠萃取物之缺點，繼續在易溶於 水之有機溶劑之濃度上進行研究。 結果，本發明之發明人在日本專利公開第3 1 6， 9 6 8/9 3號中完成且揭示純化的蜂膠萃取物，當其在 3 1 0 nm及6 6 0 nm之吸收轉化爲在2 w /w %濃 度（基於乾固體）之蜂膠萃取物之吸收時，其具有 A310nm/Aeet)nm大於4，0 0 0的吸收比，該「基於 乾固體」一詞在下文中簡稱爲「d. s. b.」，而前述 純化的蜂膠萃取物可稱爲「蜂膠萃取物」；且所獲得之蜂 經濟部中央標準局貝Η消費合作社印製 (請先閱讀背面之注意事項再填寫本頁) 膠萃取物係藉由將蜂膠之有效成份溶於濃度爲3 0 - 5 5 v/v%之易溶於水的無機溶劑，而以其級份的形式獲得 〇 在進一步研究蜂膠萃取物的過程中，發明人發現當依 習知之方法，以水稀釋蜂膠萃出物物時，蜂膠萃取物部分 變得不溶且變成含有不溶之蜂膠成份的白色懸浮液。更詳 盡之研究顯示，大部分的蜂膠有效成份，尤指物質C,皆 本紙張尺度適用中國國家標準（CNS ) Α4規格（210 X 297公釐）-6 - 470743 A7 B7 五、發明説明（4 ) 變得不溶。 進一步之研究還顯示蜂膠之有效成份易溶於濃度相對 較高或中等之溶於水無機溶劑，但是彼等在以水稀釋時， 幾乎不溶或實質上不溶於無機溶劑，如此會造成蜂膠有效 成份之活性的呈現遭到隱蔽的一系列缺點。 發明之總論 本發明之目標在於提供具有改良之水溶性、生物活性 及令人滿意之氣味及味道的蜂膠萃取物，以及其製備與用 途。爲得到如是之蜂膠萃取物，本發明之發明人對於蜂膠 萃取物作了深入之研究。 結果，本發明之發明人發現，利用添加p Η控制劑， 將蜂膠之pH 由一般的約ρΗ5調整爲5. 5或更高（ 較佳爲5. 5 — 7. 0,更好爲5. 8 — 6. 4)，作爲 蜂膠之有效成份的物質C可在水溶性上有重大之改良且其 先天所擁有之活性更易於展現，且因而得到具有改良之安 定性、氣味及味道的蜂膠萃取物。 經濟部中央標率局貝T;消费合作社印製 (請先聞讀背面之注意事項再填寫本頁) 詳而言之，本發明之發明人發現到，當依習知之方法 ，用水以十倍稀釋pH經控制之蜂膠萃取物（正常pH爲 約5. 0且含有l〇w/w%(d. s . b)蜂膠萃取物 ，於5 Ow/w%乙醇水溶液中）時，物質C溶於稀乙醇 水溶液的濃度在25 °C下僅爲〇. 〇3ppm,且藉由將 PH由約5. 〇增加爲5. 5—7. 0，物質C在稀乙醇 水溶液中的溶解度可增加至少15—100倍，此外，物 本紙张尺度適用中國國家標準（CNs ) A4規格（210 X 297公釐）_ 7 470749 Α7 Β7 五、發明説明（5 ) 質C先天所擁有之活性亦易於呈現出來。 發明之詳細說明 本發明係關於水溶性改良的蜂膠萃取物，詳而言之， 本案係關於蜂膠有效成份之水溶性藉由將蜂膠或其萃取物 之P Η調爲5 . 5 — 7而獲改良的蜂膠萃取物，其製備及 其用途’還關於供改良蜂膠萃取物之水溶性的方法。 任何方法皆可用於本發明，只要本發明之蜂膠萃取物 可藉由將蜂膠之pH調整爲5. 5—7. 0(宜爲5. 8 一 6 · 4 )而製得即可。 一般而言，本發明之蜂膠萃取物可由下列任一方法製 得，其一係藉由令蜂膠與p Η未經控制之水溶性有機溶劑 的水溶液接觸，以予以溶解並萃取蜂膠之有效成份，利用 pH控制劑將蜂膠萃取物之ΡΗ調整爲5. 5 — 7. 0並 收集萃取物；另一爲令蜂膠與pH已調爲5. 5或更高（ 較佳爲pH在5. 5—8. 0之間）之易溶於水有機溶劑 的水溶液接觸，以予以溶解並萃取蜂膠之有效成份，以及 視情況需要，在收集萃取物之前，用pH控制劑將蜂膠萃 取物之pH調整爲5. 5—7. 0。在此情況下，易溶於 水之有機溶劑在水溶液中的濃度通常爲70 v/v%或 更高。爲獲得純化的蜂膠萃取物，依日本專利公開第 316, 968/93號之方法，使用30 — 55 ν / ν %之易溶於水有機溶劑的水溶液。令蜂膠與該水溶液接 觸以及萃取蜂膠之有效成份的方法如下：溶解及萃取的方 本紙張尺度適用中國國家標準（CNS ) Α4規格（210Χ297公釐） (請先閲讀背面之注意事項再填寫本頁) -° 經濟部中央標準局員工消费合作杜印製 470749 A7 經濟部中央標準局負工消费合作社印製 B7五、發明説明（6 ) 法係包含將蜂膠置於容器中，並將易溶於水之有機溶劑倒 入容器中。逆流萃取法由於萃取效率高，適用於工業上的 製造；逆流萃取法係包含將一系列已有蜂膠施用於其上的 層柱串連以及將易溶於水之有機溶劑饋於層柱上。視需要 ，用水稀釋含有蜂膠有效成份之30-55v/v%易 溶於水有機溶劑的水溶液，並令稀釋液與粗孔狀合成吸附 劑接觸，以將有效成份吸附於其上，接著將有效成份自吸 附劑洗提出來，以獲得具有改良水溶性的蜂膠萃取物。 蜂膠萃取物之pH值係調爲5. 5 — 7. 0 (宜爲 5 . 8 — 6 . 4 )，以獲得具有改良水溶性、安定性、氣 味及味道的高品質蜂膠萃取物。當蜂膠之pH低於 5. 5時，物質C之水溶性未能令人滿意。當蜂膠萃取物 之pH超過7. 0時，萃取物之顏色更深且品質更易惡化 ,而其他的成份，如苯丙酸4 — (2 —羧酸乙烯基）一 2 一（3 —甲基一 2 —丁烯基）苯酯及黃酮類化合物會變得 不安定。這些皆會使蜂膠萃取物之品質、氣味及味道在儲 存期間惡化。 任何P Η控制劑皆可用於本發明，只要其能夠控制蜂 膠萃取物之 pH值即可，詳而言之，只要可將濃度不小 於2w/w%(d. s. b)之蜂膠萃取物之pH值調至 本發明所特定之P Η值者即可。例如，鹼性物質，諸如， 氧化鎂、氧化鈣、氫氧化鈉、氫氧化鉀、氫氧化鎂、氫氧 化鈣、碳酸氫鈉、碳酸氫鉀、碳酸鈉及碳酸鎂。視需要， 此等鹼性物質可與其他金屬鹽類，諸如氫氧化鐵、碳酸鋅 (請先閱讀背面之注意事項再填寫本頁) 本紙张尺度適用中國國家標準（CNS ) Α4規格（210X297公釐） 9 470749 經濟部中央標準局員工消费合作社印製 Α7 Β7 五、發明説明（7 ) 及碳酸銅等組合使用，且依情況需要，更可與酸類，諸如 氫氯酸、磷酸、乙酸及檸檬酸等合併使用。 在pH控制劑中，可食的且藥學上可接受的緩衝劑可 更令人滿意地以可達成本發明之目標的用量用於本發明， 可用量通常爲小於10 w/w% ( d . S. b.,基於 蜂膠萃取物）。在控制pH值後，視需要，可將蜂膠萃取 物過濾及離心，以獲得蜂膠之有效成份，諸如，物質C。 如是所得之本發明蜂膠萃取物爲2 — 2 5w/w% ( d . s · b .)蜂膠溶液，含有水溶性改良之作爲蜂膠有 效成份的物質C，且視需要，該溶液可任意地濃縮及粉化 爲粉狀的蜂膠萃取物。此外，呈液體形式之蜂膠濃縮物可 藉由與無水糖類（諸如無水麥芽糖、無水海藻糖及無水乳 糖）及/或諸如α_，/3 -及r —環糊精之物質混合，以 任意地脫水及粉化。在粉化過成程中，藉由使用p Η控制 劑（諸如碳酸氫鈉及碳酸鎂），可使最終產物中所含之蜂 膠有效成份的水溶性獲更進一步改善。 由於本發明之蜂膠萃取物所含之有效成份係呈易於被 活體所吸收及同化的形式，因此，其可單獨使用或與一或 多種其他物質合併使用，諸如，抗氧化劑、安定劑、增味 劑、染色劑、增氣味劑、填料、佐劑及賦形劑。尤其是， 當本發明之蜂膠萃取物與一或多種維生素（諸如，維生素 Ε、類胡蘿蔔素、L 一抗壞血酸、α -糖基一 L 一抗壞血 酸 '芸香素及糖基維生素Ρ)、有機酸（諸如，檸檬 酸及蘋果酸）、以及糖類（諸如，葡萄糖、麥芽糖、海藻 本紙張尺度適用中國國家樣準（CNS ) Μ規格（210Χ297公釐）_ _ (請先閲讀背面之注意事項再填寫本頁) 、11 -¾. 470749 經濟部中央標準局負工消费合作社印製 A7 B7 五、發明説明（8 ) 糖、乳糖及甘露糖醇）併用，則其活性、功效、安定性及 可處理性可獲得令人滿意的改良。 由於本發明之蜂膠萃取物展現出蜂膠有效成份先天所 擁有的活性，因此，其可單獨或與其他物質合併用於組成 物中，諸如，食品、化粧品及抗易感受性疾病之藥劑，作 爲健康食品之補充劑、防腐劑、抗氧化劑、抗發炎劑、局 部麻醉劑、病毒生長抑制劑、免疫控制劑、巨噬細胞活化 劑、抗腫瘤劑、毛髮恢復劑、抗脂血症劑、紫外線吸收劑 、黃色劑、維生素P補充劑、品質改善劑或增味劑，以維 持及促進健康、預防及/或治療疾病、自疾病狀態恢復健 康、以及維護並改善皮膚狀況。 本發明之蜂膠萃取物可自由地用於近年已廣泛爲人所 知且盛行之尿液治療法，作爲尿液治療之補充劑，以改善 效果及減少令人不悅之尿味。 本發明之蜂膠萃取物具有討喜之顏色、氣味及味道， 因此，其可與具有鹹味、澀味、或苦味的物質和合得很好 ，且具有強的防腐性。故，本發明之蜂膠萃取物不但可用 於特定的健康食品，亦可用於各種組成物，諸如一般食品 ，包括調味料、日式點心、西式糕餅、冰凍果子露 '冰棋 淋、飲料、塗於麵包上的食品、麵團、醃漬物、加工肉品 、加工魚品、牛奶及蛋類製品、加工蔬菜、加工水果 '以 及加工系列食品，以改善彼等之氣味、味道、儲存期及品 質安定性。 本發明之蜂膠萃取物亦可用於家畜、家禽、蜜蜂、蠶 (請先閱讀背面之注意事項再填寫本頁)470749 A7-B7 V. Description of the invention (1) Field of the invention The present invention relates to propolis extracts with improved water solubility. In particular, this case relates to the water solubility of the active ingredients of propolis by extracting propolis. The improved pH of the propolis extract was adjusted to 5.5-7, its preparation and its use, and a method for improving the water solubility of the propolis extract. Descriptions of conventional skills such as Propolis in Natural Therapeutics (1 9 8 3), second revision (published by Librairie Maloine SA Editeur, Paris, France), Fragrance Journal, No. (Please read the precautions on the back before filling this page ) 8 3, ρρ. 2 0 — 2 8 and pp. 3 6 -4 3 (1 9 8 7 and Ap i do logie, Vol. 2 2, pp. 15 5 — 16 2 (, νβ 1 9 9 1) Among them, propolis is a resinous product, which is stored in honeycombs by bees, contains resin, beeswax, essential oils, pollen and flavonoids, and has been used for various folk medicines for a long time. The main activities of propolis printed by consumer cooperatives are antiseptic activity, antioxidant activity, anti-inflammatory activity, local numbness, activity to inhibit virus growth, immune control activity, and macrophage activation activity. They are known and the main activity of propolis The ingredients are also known as flavonoids, aromatic carboxylic compounds, and aromatic aldehydes. Prior to the present invention, the inventors of the present invention have studied the effective ingredients of propolis. An effective ingredient, 3— [4-hydroxy-3,5—bis (3-methyl-2-butenyl) phenyl] -2—acrylic This paper is sized to the Chinese National Standard (CNS) A4 specification (210 X 297 mm)-4-470749 Α7 Β7 5. Description of the invention (2) Acid, aliased as "Ar tep i 1 1 in C" (hereinafter referred to as "substance C"), exhibits antiseptic activity and hair growth Activity and antitumor activity, and thus completed the invention related to the use of substance C: for example, Japanese Patent Publication No. 256, 177/94 discloses a preservative containing substance c as an effective ingredient, Japanese Patent Publication No. 3 1 2 '9 No. 1/8/9 discloses a hair restorer containing substance C as an active ingredient, and Japanese Patent Publication No. 191, 0/5/95 discloses an antitumor agent containing substance C as an active ingredient. As previously mentioned It is stated that the inventor of this case has confirmed that substance C is one of the important substances in propolis. Since propolis is a dark brown block product and because it is mainly composed of water-insoluble hydrophobic products, it cannot be easily used, And usually in the form of a liquid propolis extract Or they are used in the form of propolis odor. They are obtained by dissolving propolis in a high concentration of a solution easily soluble in water and organic solvents, and extracting with the solution. The solvent can be, for example, acetone, acetic acid, and Lower alcohols, such as methanol, ethanol, isopropanol, etc. Generally speaking, ethanol is the best. Consumption by the Central Bureau of Standards of the Ministry of Economic Affairs. Cooperative printing (please read the precautions on the back before filling out this page). However, if the extract is used in food (such as health food), anti-susceptible medicines (such as Treatment and / or preventive agents for susceptible diseases), and cosmetics (such as skin refining agents and skin whitening agents) have the following disadvantages, making propolis difficult to use in various fields. (1) The ingredients (such as resin and wax) of propolis extract are easy to crystallize. When the propolis extract is diluted with saliva, its ingredients are easy to crystallize. The paper size is applicable to the Chinese National Standard (CNS) A4 specification (2 丨 〇 X 297 mm)-5-470749 A7 B7. 5. Description of the invention (3), this will Strongly inhibits their absorption and utilization by living organisms. When the propolis extract is diluted with water, the components of the propolis will crystallize in large quantities, making handling very difficult; (2) the color of the propolis extract is not satisfactory and the extract is liable to become dark brown or opaque; and (3 ) The odor and taste of the propolis extract are unsatisfactory, that is, it has an earthy smell and a pungent or pungent taste. In order to overcome the disadvantages of these conventional propolis extracts, research has continued on the concentration of water-soluble organic solvents. As a result, the inventors of the present invention completed and revealed the purified propolis extract in Japanese Patent Laid-Open No. 3 16,96,8 / 9 3, and when its absorption at 3 1 0 nm and 6 6 0 nm was converted to When the propolis extract of 2 w / w% concentration (based on dry solids) is absorbed, it has an absorption ratio of A310nm / Aeet) nm greater than 4,000. The term "based on dry solids" is abbreviated as "dsb" ", And the aforementioned purified propolis extract can be referred to as" propolis extract "; and obtained by the bee consumer cooperative of the Central Standards Bureau of the Ministry of Economics (please read the precautions on the back before filling this page). It is obtained by dissolving the effective ingredients of propolis in a water-soluble inorganic solvent with a concentration of 30-5 5 v / v%, and obtaining it in the form of fractions. In the process of further studying the propolis extract, the invention It has been found that when the propolis extract is diluted with water according to a conventional method, the propolis extract partly becomes insoluble and becomes a white suspension containing insoluble propolis ingredients. A more detailed study shows that most of the active ingredients of propolis, especially substance C, are in accordance with the Chinese national standard (CNS) A4 specification (210 X 297 mm) -6-470743 A7 B7. 5. Description of the invention (4 ) Becomes insoluble. Further research has also shown that the effective ingredients of propolis are easily soluble in relatively high or medium soluble inorganic solvents, but they are almost insoluble or substantially insoluble in inorganic solvents when diluted with water. The appearance of its activity is hidden by a series of shortcomings. SUMMARY OF THE INVENTION The object of the present invention is to provide a propolis extract having improved water solubility, biological activity, and satisfactory odor and taste, as well as its preparation and use. In order to obtain such a propolis extract, the inventors of the present invention conducted intensive research on the propolis extract. As a result, the inventors of the present invention have found that the pH of propolis is adjusted from about ρΗ5 to 5.5 or higher (preferably 5.5 to 7. 0, more preferably 5. by adding p p control agent). 8 — 6. 4), Substance C, which is an effective ingredient of propolis, can have a significant improvement in water solubility and its inherently possessed activity is easier to exhibit, and thus a propolis extract with improved stability, odor and taste can be obtained Thing. Central Standards Bureau of the Ministry of Economic Affairs; printed by consumer cooperatives (please read the precautions on the back before filling out this page). In detail, the inventor of the present invention found that when using conventional methods, When diluted propolis extract with controlled pH (normal pH is about 5.0 and contains 10w / w% (d. S.b) propolis extract in 5 Ow / w% ethanol aqueous solution), substance C is dissolved The concentration in the dilute ethanol aqueous solution is only 0.03 ppm at 25 ° C, and by increasing the pH from about 5.0 to 5.5-7.0, the solubility of the substance C in the dilute ethanol aqueous solution can be increased by at least 15-100 times. In addition, the size of the physical paper is in accordance with Chinese National Standards (CNs) A4 (210 X 297 mm) _ 7 470749 Α7 Β7. 5. Description of the invention (5) The inherent activity of quality C is easy to show. . Detailed description of the invention The present invention relates to propolis extracts with improved water solubility. In particular, this case is about the water solubility of the active ingredients of propolis by adjusting the P of propolis or its extract to 5.5 to 7. Improved propolis extract, its preparation and use 'It also relates to a method for improving the water solubility of a propolis extract. Any method can be used in the present invention, as long as the propolis extract of the present invention can be prepared by adjusting the pH of the propolis to 5.5-7.0 (preferably 5. 8-6 · 4). In general, the propolis extract of the present invention can be prepared by any of the following methods. One is to contact propolis with an aqueous solution of pp uncontrolled water-soluble organic solvent to dissolve and extract the effective ingredients of propolis. The pH of the propolis extract was adjusted to 5.5 with a pH control agent and the extract was collected; the other is to make the propolis and pH have been adjusted to 5.5 or higher (preferably at pH 5.5. 8. 0) contact with an aqueous solution easily soluble in water and organic solvents to dissolve and extract the effective ingredients of propolis, and if necessary, adjust the pH of the propolis extract to pH with a pH control agent before collecting the extract. 5. 5-7. 0. In this case, the concentration of the water-soluble organic solvent in the aqueous solution is usually 70 v / v% or more. In order to obtain a purified propolis extract, according to the method of Japanese Patent Laid-Open No. 316, 968/93, an aqueous solution of 30-55 ν / ν% easily soluble in water and organic solvents is used. The method for bringing propolis into contact with the aqueous solution and extracting the effective ingredients of propolis is as follows: The size of the paper used for dissolution and extraction is applicable to the Chinese National Standard (CNS) A4 specification (210 × 297 mm) (Please read the precautions on the back before filling in this page )-° Printed by the Central Consumers' Bureau of the Ministry of Economic Affairs for consumer cooperation Du printed 470749 A7 Printed by the Central Bureau of Standards of the Ministry of Economic Affairs for printed consumer cooperatives B7 V. Description of the invention (6) The legal system consists of placing propolis in a container and making it easily soluble The organic solvent of water was poured into a container. The counter-current extraction method is suitable for industrial manufacturing because of its high extraction efficiency. The counter-current extraction method includes a series of layered columns in which a series of existing propolis is applied, and a water-soluble organic solvent is fed to the layered column. If necessary, dilute 30-55v / v% easily soluble organic solvent in water containing the active ingredient of propolis with water, and contact the diluted solution with a coarse-pored synthetic adsorbent to adsorb the active ingredient thereon, and then The ingredients are eluted from the adsorbent to obtain a propolis extract with improved water solubility. The pH of the propolis extract is adjusted to 5.5-7. 0 (preferably 5.8-6.4) to obtain a high-quality propolis extract with improved water solubility, stability, odor and taste. When the pH of propolis is below 5.5, the water solubility of substance C is not satisfactory. When the pH of the propolis extract exceeds 7.0, the color of the extract is darker and the quality is more likely to deteriorate, and other ingredients, such as phenylpropionic acid 4-(2-carboxylic acid vinyl)-2-(3-methyl- 2-butenyl) phenyl esters and flavonoids can become unstable. These all deteriorate the quality, odor and taste of the propolis extract during storage. Any P Η control agent can be used in the present invention, as long as it can control the pH value of the propolis extract, in detail, as long as the pH value of the propolis extract having a concentration of not less than 2w / w% (ds b) can be controlled. It is only necessary to adjust to the P value specified in the present invention. For example, alkaline substances such as magnesium oxide, calcium oxide, sodium hydroxide, potassium hydroxide, magnesium hydroxide, calcium hydroxide, sodium bicarbonate, potassium bicarbonate, sodium carbonate, and magnesium carbonate. If necessary, these alkaline substances can be combined with other metal salts, such as iron hydroxide, zinc carbonate (please read the precautions on the back before filling this page) This paper size applies to China National Standard (CNS) Α4 specification (210X297) (%) 9 470749 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Description of Invention (7) and copper carbonate, etc., and can be used with acids such as hydrochloric acid, phosphoric acid, acetic acid, and lemon according to the needs of the situation Acids are used in combination. Among the pH controlling agents, edible and pharmaceutically acceptable buffers can be more satisfactorily used in the present invention in an amount that can achieve the target of the present invention, and the usable amount is usually less than 10 w / w% (d. S. b., Based on propolis extract). After controlling the pH, if necessary, the propolis extract can be filtered and centrifuged to obtain the effective ingredients of propolis, such as substance C. If the obtained propolis extract of the present invention is a 2-2 5 w / w% (d. S · b.) Propolis solution, which contains substance C as an active ingredient of improved propolis, and the solution can be optionally concentrated as required And powdered propolis extract. In addition, the propolis concentrate in liquid form can be arbitrarily dehydrated by mixing with anhydrous sugars (such as anhydrous maltose, anhydrous trehalose, and anhydrous lactose) and / or substances such as α_, / 3-and r-cyclodextrin. And flouring. During the pulverization process, the water solubility of the active ingredients of propolis contained in the final product can be further improved by using pΗ control agents such as sodium bicarbonate and magnesium carbonate. Since the active ingredients contained in the propolis extract of the present invention are in a form that is easily absorbed and assimilated by the living body, they can be used alone or in combination with one or more other substances, such as antioxidants, stabilizers, flavor enhancers Agents, colorants, odorants, fillers, adjuvants and excipients. In particular, when the propolis extract of the present invention is combined with one or more vitamins (such as vitamin E, carotenoids, L-ascorbic acid, α-glycosyl-L-ascorbic acid 'rutin and glycovitamin P), organic acids ( Such as citric acid and malic acid), and sugars (such as glucose, maltose, seaweed) The paper size is applicable to China National Standard (CNS) M specifications (210 × 297 mm) _ _ (Please read the precautions on the back before filling in this Page), 11-¾. 470749 A7 B7 printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (8) Sugar, lactose and mannitol) combined use, its activity, efficacy, stability and handleability A satisfactory improvement can be obtained. Since the propolis extract of the present invention exhibits the innate activity possessed by the active ingredients of propolis, it can be used alone or in combination with other substances in the composition, such as foods, cosmetics, and anti-allergic agents, as health food Supplements, preservatives, antioxidants, anti-inflammatory agents, local anesthetics, virus growth inhibitors, immune control agents, macrophage activators, antitumor agents, hair restorers, antilipemia agents, ultraviolet absorbers, Yellow agents, vitamin P supplements, quality improvers or flavor enhancers to maintain and promote health, prevent and / or treat diseases, restore health from disease states, and maintain and improve skin conditions. The propolis extract of the present invention can be freely used in a urine therapy method which has been widely known and popular in recent years, and can be used as a supplement for urine therapy to improve the effect and reduce the unpleasant urine taste. The propolis extract of the present invention has pleasing colors, odors, and tastes. Therefore, it can be combined well with substances having a salty, astringent, or bitter taste, and has strong antiseptic properties. Therefore, the propolis extract of the present invention can be used not only for specific health foods, but also for various compositions, such as general foods, including seasonings, Japanese dim sum, western-style pastries, frozen sherbet, iced ice cream, beverages, coated on Bread, food, dough, pickles, processed meat, processed fish, milk and egg products, processed vegetables, processed fruits' and processed foods to improve their odor, taste, shelf life and quality stability . The propolis extract of the present invention can also be used in livestock, poultry, bees, and silkworm (please read the precautions on the back before filling this page)

、1T 本紙張尺度適用標準（CNS ) A4規格（2丨0X297公襲）_ _ 470749 經濟部中央標準局員工消费合作社印製 A7 B7 五、發明説明（9 ) 及魚類之飼料及寵物食品，作爲防腐劑、腸控制劑、維生 素P補充劑、壓力舒緩劑或味道改善劑。 此外，本發明之蜂膠萃取物可任意地用於呈固體、糊 狀或液態的產品，包括化粧品及藥劑，例如，煙草、紙煙 、糖錠、綜合維生素、舌下劑、口芳香劑、口香片及漱口 水、插管餵食之營養劑、天然藥物、腸藥、注射液、牙膏 、口紅（包括用於龜裂嘴唇者）、防曬劑、洗面皂、洗髮 精、潤絲精、浴鹽、抗易感受性疾病的藥劑、皮膚細緻劑 、皮膚增白劑、及毛髮恢復劑。 本發明所謂之「抗易感受性疾病之藥劑」一詞係指供 治療及預防易感受性疾病之藥劑，且在本發明中，「易感 受性疾病」一詞係指可被本發明之蜂膠萃取物治療及/或 預防的疾病。如是易感受性疾病的例子有病毒疾病、細菌 性疾病、創傷、免疫疾病、風濕、糖尿病、循環性疾病、 惡性腫瘤及神經疾病。 本發明之抗易感受性疾病之藥劑形成適當的劑型，以 供最終用途。例如，其可形成液態的藥劑，諸如，噴散劑 、灌鼻藥、霧劑、漱口水或注射液；糊狀的藥劑，諸如軟 膏、軟食或乳劑；以及固體藥劑，諸如，粉劑、粒劑、囊 劑或片劑。 本發明藥劑之劑量的控制係取決於蜂膠萃取物之量、 投服途徑或頻率，一般而言，其係於約0. 0 0 0 1 _ 1 0 g/天/成人。 就用於化粧品及食品的情況下，本發明之蜂膠萃取物 本紙张尺度適用中國國家標準（CNS)A4規格（210X297公釐）-12- (請先閱讀背面之注意事項再填寫本頁) 訂 -^ 470749 Α7 Β7 五、發明説明（10) 係如前述之易感受性疾病藥劑之使用情形類似。 可用於將蜂膠萃取物併入本發明之組成物的方法包括 任何可將蜂膠萃取物併辱組成物者。如是方法之例有：混 合、捏和、溶解、浸水、滲透、噴霧、塗覆、敷佈及射出 〇 本發明將詳述於下文。 實施例1 丕溶物晳C之確認 將蜂膠塊瓦解，注入三個層柱中，並利用逆流萃取法 ，饋以50 V/V%乙醇水溶液，以溶解及萃取蜂膠之 有效成份。將萃取物過濾，以獲致產量約爲蜂膠原料之 30% (d. s . b .)且含有約20 w/w%乾燥固 體的液體萃取物。 將該蜂膠萃取物作爲試樣，得到其吸收比值， 經濟部中央標率局努工消费合作社印製 (請先閱讀背面之注意事項再填寫本頁) A 310nm/ A 66〇111„爲7，365。在整個說明書中，試 樣皆係以5 0 v/v%之乙醇水溶液稀釋爲2 w / w % (d. s. b)之溶液，且以1公分光徑長的玻璃小室進 行在6 6 0 nm之吸收。同樣地，試樣係稀釋爲 〇 〇 0 4 w /w % ( d . s. b.)的溶液，且於 3 1 0 n m下進行吸收的測量。將3 1 0 n m之吸收放大 5〇倍，以將其轉換爲2 w / w % ( d . s. b_ )時 之吸收，然後計算A31()nm/Ae6Qnin之比例。 用P Η測量計所測得之試樣的P Η值爲5 . 0。將試 本紙张尺度適用中國國家標準（CNS ) Α4規格（210Χ297公釐）-13 - 470749、 1T Applicable standard (CNS) A4 specification of this paper standard (2 丨 0X297 public attack) _ 470749 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 5. Invention Description (9) and fish feed and pet food, as Preservatives, bowel control agents, vitamin P supplements, stress relievers or taste improvers. In addition, the propolis extract of the present invention can be arbitrarily used in products that are solid, pasty, or liquid, including cosmetics and medicaments, such as tobacco, paper tobacco, lozenges, multivitamins, sublingual agents, mouth fragrances, and chewing tablets. And mouthwash, nutrient for intubation feeding, natural medicine, intestinal medicine, injection, toothpaste, lipstick (including those with cracked lips), sunscreen, facial soap, shampoo, conditioner, bath salt, Anti-susceptible agents, skin refining agents, skin whitening agents, and hair restoration agents. The term "medicine against susceptible disease" in the present invention refers to a medicament for the treatment and prevention of susceptible disease, and in the present invention, the term "susceptible disease" means that it can be treated by the propolis extract of the present invention And / or preventive diseases. Examples of susceptible diseases include viral diseases, bacterial diseases, trauma, immune diseases, rheumatism, diabetes, circulatory diseases, malignant tumors and neurological diseases. The medicament for the resistance to susceptible diseases of the present invention is formed into an appropriate dosage form for end use. For example, it can form liquid medicaments such as sprays, nasal medicines, mists, mouthwashes or injections; pasty medicaments such as ointments, soft foods, or emulsions; and solid medicaments such as powders, granules, Capsules or tablets. The control of the dosage of the agent of the present invention depends on the amount of propolis extract, the route of administration or the frequency. Generally speaking, it is about 0. 0 0 0 1 _ 1 0 g / day / adult. In the case of use in cosmetics and food, the paper size of the propolis extract of the present invention is applicable to the Chinese National Standard (CNS) A4 specification (210X297 mm) -12- (Please read the precautions on the back before filling this page) Order -^ 470749 Α7 Β7 V. Description of the invention (10) The use of susceptible disease agents as described above is similar. Methods that can be used to incorporate a propolis extract into the composition of the present invention include any person who can combine a propolis extract with a composition. Examples of methods are: mixing, kneading, dissolving, immersing in water, penetrating, spraying, coating, applying and spraying. The present invention will be described in detail below. Example 1 Confirmation of the soluble substance C. The propolis block was disintegrated, injected into three layers of columns, and fed with a 50 V / V% ethanol aqueous solution using a counter current extraction method to dissolve and extract the effective components of propolis. The extract was filtered to obtain a liquid extract having a yield of about 30% (d. S.b.) of the propolis raw material and containing about 20 w / w% dry solids. The propolis extract was used as a sample to obtain its absorption ratio. It was printed by Nu Gong Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) A 310nm / A 66〇111 „is 7, 365. Throughout the specification, the samples are all diluted with 50 v / v% ethanol aqueous solution to 2 w / w% (ds b) solution, and the glass chamber with a light path length of 1 cm is carried out at 6 6 0 Absorption at nm. Similarly, the sample was diluted with a solution of 0.004 w / w% (d. sb), and the absorption was measured at 3 10 nm. The absorption at 3 10 nm was amplified by 50. Times to convert it to the absorption at 2 w / w% (d. S. B_), and then calculate the ratio of A31 () nm / Ae6Qnin. The P Η value of the sample measured with the P Η meter 5.0. The paper size of this test will be in accordance with China National Standard (CNS) Α4 specification (210 × 297 mm) -13-470749

7 7 A B 五、發明説明（11) 樣置於燒杯內且用水予以稀釋101倍，而成爲呈白色懸 浮液之稀釋乙醇水溶液，然後，在溫和攪拌的條件下，令 該懸浮液於2 5 °C靜置1小時，並予以離心，以獲得上清 液。用咼效能液相層析法，依Bioscience， Biotechnology 'and Biochemistry - Vo 1. 5 8 ， No. 5 ，P P . 945 — 946 (1994)所述之方法，對上清液內之 物質C濃度進行定量測定。用乙醇取代水，將試樣稀釋 1 0 1倍以作爲對照組。在此情況下，試樣並未變成懸浮 液，且依前文所述者對該稀釋液進行物質C濃度的測量。 結果示於表1。 (請先鬩讀背面之注意事項再填寫本頁) 、tT_ 經濟部中央標準局負工消费合作社印製 表1 物質C之濃度（p pm) 以水稀釋之稀釋液的上清液 0 . 0 3 以乙醇稀釋之稀釋液的上清液 4 6. 7 (對照組） 表1之結果清楚顯示，藉由用水將蜂膠萃取物稀釋 1 0 1倍所得之稀釋乙醇水溶液上清液中所溶解之物質c 的濃度爲0 03ppm。該濃度僅爲經乙醇稀釋101 倍之對照組之46. 7ppm的0· 06%，這表示乙水 本紙张尺度適用中國國家標準（CNS ) A4规格（210X297公釐）-14 - 470749 A7 B7 經濟部中央標準局員工消费合作社印製 五、發明説明（12) 稀釋時，大部分之物質C皆不溶。 由離心所收集得之沉積物內的物質C量係同樣地藉由 將其溶解於乙醇中來測量，結果顯示沉積物中含有至少 9 9%之試樣中的物質C。 結果證實用水將蜂膠萃取物稀釋爲稀釋的乙醇水溶液 ，無法溶解大部分的物質C。 實驗2 物質C之溶解度的改良 令實驗1之試樣在有或無添加足量之氫氧化鈉的情況 下，與等量之50 v/v%乙醇水溶液混合，且在將混 合物製成爲約10 w/w%(d. s. b.)之液體蜂 膠萃取物前，將此pH爲5. 0之混合物之pH調整爲 5 . 2、5 . 5、5. 8、6. 0、6. 2、6. 4、 6. 8、 7. 0、 7. 2、 7. 5或8_ 1，以作爲下列 實施例中。 富驗2 — ( 1 ) 蜂膠萃取物之不溶件 藉由將實驗2所製得之各種不同p Η的試樣稀釋 10 1倍以得呈白色懸浮液之稀釋乙醇水溶液，並用1公 分光徑之玻璃小室，在7 2 0 n m下測量混濁度或吸收 ，以研究pH對於蜂膠萃取物之不溶性的影響。 (請先閲讀背面之注意事項再填寫本頁) 竣 本紙張尺度適用中國國家標準（CNS ) A4規格（210X 297公釐）_ j 5 470749 A7 經濟部中央標準局負工消费合作社印製 B7五、發明説明（l3) 實驗2 -（ 2 ) 蜂膠萃取物由之物晳c 藉由用水將各種不同pH之試樣稀釋10 1倍，如實 驗1 一般地將所形成之懸浮液離心，並測量所得之上清液 中物質C的濃度，以測量pH對於蜂膠萃取物中物質c 之濃度的影響。 實驗2 —（ 3 ) 顯色稈麽 各種不同pH之試樣即使在用乙醇稀釋21倍後，依 然呈溶液形式，且使用1公分光徑之玻璃小室，在4 80 n m下測量所得稀釋液之吸收，以研究p Η對於蜂膠萃取 物之顯色及惡化的影響。 實驗2 - ( 4 ) A 3 1 n r, m A f； ft r> n m 之吸收比 研究試樣之P H差異對於A 310nm/ A β6〇ηη^吸收比 的影響，以作爲保持蜂膠之品質的安定性條件之一。 實驗2 -（ 5 ) 在25°C下靜置一個月後，依實驗2_ (1)至2_ (4 )所述相同者，對試樣進行實驗並用1 2人之審查員 進行感覺評估。數據示於表2。 (請先閱讀背面之注意事項再填寫本頁)7 7 AB V. Description of the invention (11) The sample was placed in a beaker and diluted 101 times with water to form a diluted ethanol aqueous solution as a white suspension. Then, under mild stirring conditions, the suspension was allowed to stand at 2 5 ° C was left for 1 hour and centrifuged to obtain a supernatant. The concentration of Substance C in the supernatant was determined by tritium performance liquid chromatography in accordance with the method described in Bioscience, Biotechnology 'and Biochemistry-Vo 1. 58, No. 5, PP. 945-946 (1994). Quantitative determination. Water was replaced with ethanol, and the sample was diluted 101 times as a control group. In this case, the sample did not become a suspension, and the dilution C was measured for the concentration of substance C as described above. The results are shown in Table 1. (Please read the notes on the back before filling out this page), tT_ Printed by the Central Standards Bureau of the Ministry of Economic Affairs, Consumer Cooperatives, Table 1 Concentration of Substance C (p pm), the supernatant of the diluted solution diluted with water 0. 0 3 Supernatant of the diluted solution diluted with ethanol 4 6. 7 (Control group) The results in Table 1 clearly show that the dissolved ethanol in the supernatant of the diluted ethanol aqueous solution obtained by diluting the propolis extract 101 times with water The concentration of substance c was 0 03 ppm. This concentration is only 0.66% of 46.7 ppm of the control group diluted 101 times with ethanol, which means that the size of the paper is applicable to the Chinese National Standard (CNS) A4 specification (210X297 mm) -14-470749 A7 B7 Economy Printed by the Consumers' Cooperatives of the Ministry of Standards of the People's Republic of China. 5. Description of Invention (12) Most of substance C is insoluble when diluted. The amount of substance C in the sediment collected by centrifugation was also measured by dissolving it in ethanol, and the result showed that the sediment contained at least 99% of the substance C in the sample. The results confirmed that the propolis extract was diluted with water to a dilute aqueous ethanol solution and was unable to dissolve most of the substance C. Experiment 2 The improvement of the solubility of Substance C allows the sample of Experiment 1 to be mixed with an equal amount of 50 v / v% ethanol aqueous solution with or without the addition of a sufficient amount of sodium hydroxide, and the mixture is made to about 10 w / w% (dsb) before liquid propolis extract, adjust the pH of this mixture of pH 5.0 to 5.2, 5.5, 5.5, 5.8, 6.0, 6.0, 6.4 , 6.8, 7. 0, 7. 2, 7. 5 or 8-1 as the following embodiments. Rich test 2 — (1) Insoluble parts of propolis extract. Dilute the samples of various p Η prepared in Experiment 2 by 10 times to obtain a dilute aqueous ethanol solution as a white suspension. A glass cell was used to measure turbidity or absorption at 720 nm to study the effect of pH on the insolubility of propolis extract. (Please read the precautions on the back before filling this page) The paper size of this paper is applicable to the Chinese National Standard (CNS) A4 (210X 297 mm) _ j 5 470749 A7 Printed by the Consumers ’Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs Explanation of the invention (l3) Experiments 2-(2) Propolis extracts are obtained by c. Diluting various samples of different pH by 10 times with water. As in Experiment 1, the resulting suspension is centrifuged and measured. The concentration of substance C in the resulting supernatant was used to measure the effect of pH on the concentration of substance c in the propolis extract. Experiment 2 — (3) Color-developed stalks. Samples of different pHs were in the form of solutions even after being diluted 21 times with ethanol, and a glass cell with a light path of 1 cm was used, and the resulting diluted solution was measured at 4 80 nm. Absorption to study the effect of p Η on color development and deterioration of propolis extract. Experiment 2-(4) Absorption ratio of A 3 1 nr, m A f; ft r> nm The effect of pH difference of the sample on the absorption ratio of A 310nm / A β6〇ηη ^ was used as a stability to maintain the quality of propolis Sexual conditions. Experiment 2-(5) After standing at 25 ° C for one month, perform the test on the sample and perform sensory evaluation with 12 inspectors as described in Experiments 2_ (1) to 2_ (4). The data are shown in Table 2. (Please read the notes on the back before filling this page)

、1T 本紙張尺度適用中國國家標準（CNS ) A4規格（210X 297公釐）_ J 6 470749 Α7 Β7 五、發明説明（14) 在表 2 中，” X ” A” B ” 、” C，，、” D ” 及 ，’ E”所代表之意義如下：X ，蜂膠萃取物之pH; A ，在72〇nm下之混濁度（A72〇nm);B，上 清液中之物質C的濃度（ppm) ; C,上清液中之物質 C相對於試驗1 (亦即未進行PH調整之稀釋乙醇水溶液 )之物質C的濃度比；D,在4 8〇nm之顯色程度（ A 4 8 Ο n m );以及 Ε , Α3ι〇ηι„/Α6β〇ηιη 之吸收比。 ” * ”及” * * ”二符號係分別示「未進行ρ Η之控制」 及「在1 2個審查員中之1 〇個以上審查員的評估概要」 (請先閱讀背面之注意事項再填寫本頁) 經濟部中失標準局員工消费合作社印製 本紙張尺度適用中國國家標準（CNS ) Α4規格（210X29*7公釐）-17 - 470749 經濟部中央標準局K工消費合作社印製 A7 B7 發麵（15) ^ 試驗編號 製得時 置靜 一個月後 總體評分 X A B C D E A B C D E 氣味&味道 1 5. 0* 0.224 0.03 1 0.197 7,365 0.230 0.03 1 0. 210 6,918 令人滿意 不好 對照組 2 5.2 0.202 0.06 2 0.197 7,187 0. 205 0. 07 2 0. 213 6, 729 令人滿意 不好 對照組 3 5,5 0.188 0.45 15 0.198 6,514 0.188 0.45 15 0. 215 6,375 令人滿意 良好 本發明 4 5.8 0.176 2.30 77 0.204 6,434 0.180 2.38 79 0.224 6,213 令人滿意 極佳 本發明 5 6.0 0.144 4.28 143 0. 210 6,378 0.148 4.37 146 0. 231 6, 023 令人滿意 極佳 本發明 6 6.2 0.134 8.40 280 0.221 6, 015 0.136 8.48 283 0. 245 5, 682 令人滿意 極佳 本發明 7 6.4 0.101 13.6 453 0.248 5, 659 0.102 13.8 460 0. 266 5,054 令人滿意 極佳 本發明 8 6,8 0.078 18.7 623 0.260 5,401 0.078 18.9 630 0. 282 4,868幾乎令人滿意 良好 本發明 9 7.0 0. 058 24.1 803 0.283 4, 973 0.059 24.2 807 0.305 4, 054幾乎令人滿意 良好 本發明 10 7.2 0.051 28.3 943 0,303 4,614 0.053 28.3 943 0.399 3,860 稍爲刺激 不好的氣味 不好 對照組 11 7.5 0. 049 31.4 1,047 0.329 4,303 0.050 31.0 1,033 0.458 3,675 刺激， 不好的氣味 不好 對照組 12 8.1 0. 041 36.4 1,213 0. 464 2,935 0. 044 36.2 1,207 0.588 2,320 剌激， 不好的氣味 不好 對照組 (請先閱讀背面之注意事項再填寫本頁) 本紙張尺度適用中國國家標準（CNS ) Λ4规格（210X297公釐）-18 470749 A7 B7 五、發明説明（16) 表2之結果充分顯示將蜂膠之pH值由5. 0增加至 5. 5 - 7 . 0(更佳爲5. 8 — 6. 4)，可製得物質 C之水溶性改善且具有令人滿意之安定性、口味及氣味之 高品質蜂膠萃取物。 實驗2 —（ 1 )顯示隨著試樣之pH的增加，試樣之 混濁度及水溶解性亦分別隨之降低及改善；詳而言之，該 二性質會分別隨之降低及改善直至pH爲約7. 0，且在 P Η大於約7 . 0時，混濁度幾乎不在減少，亦即未發現 水溶性有實質之改善。實驗2 -（2)顯示當pH調至大 於5. 5時，物質C (其爲蜂膠之有效成份之一）之水溶 性急遽增加。實驗2 - (3)顯示尤其在pH大於7時， 試樣之顏色更深，且此一趨勢在保存期間更加明顯，因而 導致試樣之惡化。其亦顯示當試樣儲存在大於7之p Η下 時，試樣會有刺激之味道、難聞的氣味及/或惡化之氣味 及味道。 經濟部中央標準局負工消費合作杜印裂 (請先閱讀背面之注意事項再填寫本頁) 實驗2 —（4)顯示試樣必須保存在7或7以下之 pH下，以在靜置1個月後，依然保存其高品質或將其 A31〇nm/Ae6〇nm之比例維持於大於4，0 0 0。 綜合前述數據，可知藉由添加pH控制劑，將蜂膠之 pH 調爲 5. 5 — 7. 0(更佳爲 5. 8 — 6. 4)，可 製得物質C (蜂膠之有效成份）之水溶性或改善且具有令 人滿意之安定性、氣味及味道的令人滿意蜂膠萃取物。 實驗3 本紙张尺度適用中國國家標隼（CNS)A4規格（210X297公釐）-19 - 470749 經濟部中央標準局负工消费合作社印製 Α7 Β7 五、發明説明（ΐΌ 急件毒件試驗 使用七星期大之dd品系鼠，將藉由實施例Α — 1方 法所製得pH爲6. 4之液體蜂膠萃取物之冷凍乾燥產 物經口投予鼠，以進行急性毒性試驗。結果，未有任何老 鼠在高達2. 5g/ kg (鼠重）之劑量下死亡。這表示 該產物具有極低之毒性。使用藉由實施例A - 4方法所得 pH爲6. 2之液體蜂膠萃取物的冷凍乾燥產物，進行相 同的試驗，結果產生相同的結果，這表示該產物同樣具有 極低之毒性。 下文之實施例A及B係例示水溶性改善之本發明蜂膠 萃取物及含有彼作爲有效成份之組成物： 實施例A _ 1 令蜂膠浸於95 v/v%之乙醇水溶液中，且依習 知的方式，用該溶液予以萃取，用少量水清洗沉積物，以 萃取沉積物。將此等萃取物收集爲粗製的蜂膠萃取物，其 在80v/v%之乙醇水溶液中含有約20 w/w%乾 固體，然後將其與水混合，以降低乙醇之濃度至5 Ον/ ν % »令該稀釋液於5 0 °C下靜置1小時，以分離成二相 ，上層含有蜂膠之有效成份，而下層則含有黏稠之沉積物 。令此二層於周溫下靜置一整夜，並收集上層液且令其與 碳酸氫鈉混合，以將 P Η調爲6 . 4，接著回收A 31C)nm /Αβ60ηη^收比爲8，4 2 5且水溶性改良的液體蜂膠 萃取物，其產率爲約4 5 % (相對於粗製蜂膠萃取物， 本紙张尺度適用中國國家標準（CNS)A4規格（ 210X297公釐）-20 - (請先閱讀背面之注意事項再填寫本頁) 訂 -f 470749 經濟部中央標準局貝工消費合作杜印製 A7 B7 五、發明説明（18) d . s . b . ) ° 此含有水溶性改良之物質C且具有令人滿意之安定性 、氣味及味道的產物可任意地單獨或與其他物質組合，以 用於食品、抗易感受性疾病之藥劑及化粧品，作爲健康食 品補充劑、防腐劑、抗氧化劑、免疫控制劑、巨噬細胞活 化因子、抗腫瘤劑或毛髮恢復劑。 實施例A — 將蜂膠塊打散且注射至二層柱中，然後將50 v / v%之乙醇水溶液饋入層柱中，以溶解且藉由逆流萃取法 萃取蜂膠之有效成份。將所得之萃取物過濾且令其與碳酸 鈉混合，以將pH調爲6. 2。由此可獲得A 31C)nin/ A ^^。㈣吸收比爲6，0 1 5且具有改良之水溶性的液體 蜂膠萃取物，其產率爲約3 2 %(相對於蜂膠起始物， d s . b . ) 〇 與實施例A-1之產物相同者，此產物含有水溶性改 良之物質C且具有令人滿意之安定性、氣味及味道。因 而，可任意地單獨或與其他物質組合，以用於食品、抗易 感受性疾病之藥劑及化粧品，作爲健康食品補充劑、防腐 劑、抗氧化劑、免疫控制劑、巨噬細胞活化因子、抗腫瘤 劑或毛髮恢復劑。 實施例A — 3 將蜂膠塊打散且注射至三層柱中，然後將50 v / 本紙張尺度適用中國國家標準（CNS ) A4規格（210X297公釐）_ 21 (請先閱讀背面之注意事項再填寫本頁)、 1T This paper size applies Chinese National Standard (CNS) A4 specification (210X 297mm) _ J 6 470749 Α7 Β7 V. Description of invention (14) In Table 2, “X” A ”B”, “C”, "D" and "E" represent the following meanings: X, pH of propolis extract; A, turbidity at 72nm (A72nm); B, concentration of substance C in the supernatant (Ppm); C, the concentration ratio of substance C in the supernatant relative to substance C of test 1 (that is, diluted ethanol aqueous solution without pH adjustment); D, the degree of color development at 480 nm (A 4 8 〇 nm); and the absorption ratios of Ε, Α3ι〇ηι „/ Α6β〇ηιη." * "And" * * "two symbols respectively indicate" the control of ρ Η is not performed "and" in the 12 examiners Summary of evaluation of more than 10 examiners "(Please read the notes on the back before filling out this page) Printed on the paper standard printed by the Employees' Cooperatives of the China Standards Bureau of the Ministry of Economic Affairs Applicable to China National Standard (CNS) A4 Specification (210X29 * 7mm) -17-470749 A7 B7 printed by K Industrial Consumer Cooperatives, Central Standards Bureau, Ministry of Economic Affairs (15) ^ Test number system Overall score XABCDEABCDE Odor & Taste 1 5. 0 * 0.224 0.03 1 0.197 7, 365 0.230 0.03 1 0. 210 6,918 Satisfactory control group 2 5.2 0.202 0.06 2 0.197 7,187 0. 205 0. 07 2 0. 213 6, 729 Satisfactory and poor control group 3 5,5 0.188 0.45 15 0.198 6,514 0.188 0.45 15 0. 215 6,375 Satisfactory and good The present invention 4 5.8 0.176 2.30 77 0.204 6,434 0.180 2.38 79 0.224 6,213 Satisfactory excellent invention 5 6.0 0.144 4.28 143 0. 210 6,378 0.148 4.37 146 0. 231 6, 023 Satisfactory excellent invention 6 6.2 0.134 8.40 280 0.221 6, 015 0.136 8.48 283 0. 245 5, 682 makes Satisfactory excellent present invention 7 6.4 0.101 13.6 453 0.248 5, 659 0.102 13.8 460 0. 266 5,054 Satisfactory excellent present invention 8 6,8 0.078 18.7 623 0.260 5,401 0.078 18.9 630 0. 282 4,868 Almost satisfactory good present invention Invention 9 7.0 0. 058 24.1 803 0.283 4, 973 0.059 24.2 807 0.305 4, 054 almost satisfactory Good invention 10 7.2 0.051 28.3 943 0,303 4,614 0.053 28.3 943 0.399 3,860 slightly irritating Control group with bad odor 11 7.5 0. 049 31.4 1,047 0.329 4,303 0.050 31.0 1,033 0.458 3,675 stimulation, bad odor bad control group 12 8.1 0. 041 36.4 1,213 0. 464 2,935 0. 044 36.2 1,207 0.588 2,320 Irritated Control group with bad smell and bad smell (please read the precautions on the back before filling this page) This paper size applies to Chinese National Standard (CNS) Λ4 specification (210X297 mm) -18 470749 A7 B7 V. Description of the invention (16 The results in Table 2 fully show that increasing the pH value of propolis from 5.0 to 5.5-7.0 (more preferably 5. 8-6. 4) can improve the water solubility of substance C and have the High-quality propolis extract with satisfactory stability, taste and odor. Experiment 2— (1) shows that as the pH of the sample increases, the turbidity and water solubility of the sample decrease and improve respectively; in detail, these two properties will decrease and improve until the pH It is about 7.0, and when P Η is greater than about 7.0, the turbidity is hardly reduced, that is, no substantial improvement in water solubility is found. Experiments 2-(2) showed that when the pH was adjusted to more than 5.5, the water solubility of substance C, which is one of the effective ingredients of propolis, increased sharply. Experiments 2-(3) showed that the color of the sample was darker especially when the pH was higher than 7, and this tendency was more obvious during storage, which resulted in the deterioration of the sample. It also shows that when the sample is stored under p 大于 greater than 7, the sample will have an irritating taste, an unpleasant odor, and / or a deteriorated odor and taste. Du Yinli, Co-operation and Consumer Cooperation of the Central Bureau of Standards, Ministry of Economic Affairs (please read the precautions on the back before filling out this page) Experiment 2 — (4) The sample must be stored at a pH of 7 or below in order to stand at 1 After a month, its high quality was still preserved or its A31nm / Ae60nm ratio was maintained at more than 4,000. Based on the above data, it can be known that by adding a pH control agent, the pH of propolis can be adjusted to 5.5 — 7.0 (more preferably 5. 8 — 6. 4), and the substance C (the effective ingredient of propolis) can be obtained. Satisfactory propolis extract that is water soluble or improved and has satisfactory stability, odor, and taste. Experiment 3 This paper size is in accordance with Chinese National Standard (CNS) A4 (210X297 mm) -19-470749 Printed by the Consumers ’Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs Α7 Β7 V. Description of the invention The Dazhi dd strain rat was orally administered with a freeze-dried product of a liquid propolis extract having a pH of 6.4 prepared by the method of Example A-1 to the rat for an acute toxicity test. As a result, there were no rats It died at a dose of up to 2.5 g / kg (rat weight). This indicates that the product has extremely low toxicity. The freeze-dried product of a liquid propolis extract with a pH of 6.2 obtained by the method of Examples A-4 was used. The same tests were carried out, and the results produced the same results, which indicates that the product also has extremely low toxicity. Examples A and B below exemplify the propolis extract of the present invention with improved water solubility and a composition containing the same as an active ingredient. : Example A _ 1 Propolis was immersed in a 95 v / v% ethanol aqueous solution, and the solution was extracted in a conventional manner, and the sediment was washed with a small amount of water to extract the sediment. The material was collected as a crude propolis extract, which contained about 20 w / w% dry solids in an 80v / v% ethanol aqueous solution, and then mixed it with water to reduce the concentration of ethanol to 5 Ον / ν% »Let the The diluent was allowed to stand at 50 ° C for 1 hour to separate into two phases. The upper layer contained the active ingredients of propolis, while the lower layer contained thick deposits. Allow the two layers to stand overnight at ambient temperature, and The upper liquid was collected and mixed with sodium bicarbonate to adjust PΗ to 6.4, and then recovered A 31C) nm / Aβ60ηη ^ liquid propolis extract with a water solubility improved of 8, 4 2 5 which was Yield is about 45% (compared with crude propolis extract, this paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) -20-(Please read the precautions on the back before filling this page)) Order- f 470749 A7 B7 printed by Shellfish Consumer Cooperation of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (18) d. s. b.) ° This substance contains water-improved substance C and has satisfactory stability, odor and Taste products can be used alone or in combination with other substances for food, anti-susceptibility Drugs and cosmetics for affected diseases, as health food supplements, preservatives, antioxidants, immune control agents, macrophage activating factors, antitumor agents or hair restorers. Example A — The propolis block was dispersed and injected into a two-layer column, and then a 50 v / v% ethanol aqueous solution was fed into the layer column to dissolve and extract the effective components of propolis by a countercurrent extraction method. 2。 The resulting extract was filtered and mixed with sodium carbonate to adjust the pH to 6.2. Thus, A 31C) nin / A ^^ can be obtained. ㈣ Liquid propolis extract with an absorption ratio of 6,0 15 and improved water solubility, and its yield is about 32% (relative to the propolis starting material, ds.b.). If the product is the same, this product contains substance C with improved water solubility and has satisfactory stability, odor and taste. Therefore, it can be used arbitrarily or in combination with other substances for food, anti-susceptible medicines and cosmetics, as health food supplements, preservatives, antioxidants, immune control agents, macrophage activating factors, anti-tumor Agent or hair restorer. Example A — 3 Break and inject the propolis block into a three-layer column, and then apply 50 v / this paper size to the Chinese National Standard (CNS) A4 specification (210X297 mm) _ 21 (Please read the precautions on the back first (Fill in this page again)

、tT 470749 A7 B7 經濟部中央標率局貞工消费合作社印製 五、發明説明（19) v%之乙醇水溶液（其已先以碳酸鈉將PH調爲7. 8) 饋入層柱中，以溶解且藉由逆流萃取法萃取蜂膠之有效成 份。用pH控制劑將萃取物之PH調爲6. 2且予以過濾 ，以獲得A 310nin/ A 6 6 0 nm吸收比爲6，4 3 8且具有 改良之水溶性的液體蜂膠萃取物。其產率爲約3 0 % (相 對於蜂膠起始物，d. s.b.)。 與實施例A-1之產物相同者，此產物含有水溶性改 良之物質C且具有令人滿意之安定性、氣味及味道。因而 ，可任意地單獨或與其他物質組合，以用於食品、抗易感 受性疾病之藥劑及化粧品，作爲健康食品補充劑、防腐劑 、抗氧化劑、免疫控制劑、巨噬細胞活化因子、抗腫瘤劑 或毛髮恢復劑。 實施例A - 4 依實施例A-1之方法，將藉由實施例A_1方法所 製得之含有粗製蜂膠萃取物之8 Ο v/v %乙醇水溶液的 乙醇濃度降低爲4 5 v/v%，以分離爲二相，亦即上、 下二層，令該二層於周溫下靜置5小時。收集上層且用等 量水予以稀釋，並將該稀釋液饋入填裝有” AMBERLI TEX AD - 7 " ( Rohm & Haas Co. * Philadelph- ia， Pa. ， USA所出產之合成巨孔樹脂）的層柱中，用 水予以清洗，並饋入9 5 v / v %之乙醇水溶液，以洗提 蜂膠之有效成份。令所得之洗提物與碳酸氫鈉混合，以將 P Η調爲6 _ 2，而獲得A310nm/ AetJOnm吸收比爲 (請先閲讀背面之注意事項再填寫本頁) 、1T- 本紙张尺度適用中國國家標準（CNS ) Α4規格（210x297公釐）-22 - 470749 經濟部中央標準局—工消費合作社印" A7 B7 五、發明説明（2〇 ) 21， 892且具有改良之水溶性的液體蜂膠萃取物。其 產率爲約22% (相對於蜂膠起始物，d. s . b .)。 與實施例A-1之產物相同者，此產物含有水溶性改 良之物質C且具有令人滿意之安定性、氣味及味道。因 而，可任意地單獨或與其他物質組合，以用於食品、抗易 感受性疾病之藥劑及化粧品，作爲健康食品補充劑、防腐 劑、抗氧化劑、免疫控制劑、巨噬細胞活化因子、抗腫瘤 劑或毛髮恢復劑。 實施例B - 1 膠軟糖（Gummy Candy) 在減壓下，將1 Ο 0重量份” MA BIT® (―種氫化麥 芽糖漿，HayashibaraShoji， Inc. ' Okayama ' Jap-an之產品）及5 0重量份之” TREHA0SETM” （ 一種高純度 、含水之晶狀海藻糖，HayashibaraShoji， Inc. Okayama’ Japan之產品）加熱及濃縮，而成爲水份爲約 15w/w%之濃體。依習知的方式，令該濃體與13重 量份之明膠（溶於1 8重量份的水中）、1重量份由實施 例A- 1之方法所得之液體蜂膠萃取物、2重量份檸檬酸 鈉、以及足量之色料及增味劑。將如是所製得之混合物塑 型及包裝爲所需之產物》 該產物爲具有令人滿意之口感及味道且不會誘發蛀牙 的膠軟糖。 本紙依尺度適闲中國國家標準（CNS ) A4規格（210X297公釐）-23- (請先閱讀背面之注意事項再填寫本頁) 訂 470749 A7 B7 經濟部中央標準局負工消費合作社印製 五、發明説明（21 ) 眚施例B — 2 口香糖 加熱3重量份之樹膠基質直至其融化爲止，並將結果所 得之物質與4重量份蔗糖及3重量份” TREHAOSETM ”、 0. 0 2重量份由實施例A-1之方法所得液體蜂膠萃 取物，以及足量之色料混合，並用輥捏和該混合物，再予 以塑型及包裝爲所需之產物。 該產物爲具有令人滿意之口感、氣味及味道且較不會 引起蛀牙的口香糖，因其含有蜂膠之有效成份。 眚施例B — 3 G V u h i (澱粉糊1 令1重量份之糯米澱粉與1. 2重量份之水混合，並 藉由加熱使所得之混合物明膠化，再進一步與1. 5重量 份之” TREHA0SETM ” 、0 . 7 重量份之” SUNMALT®、含 水晶狀麥芽糖（Hayashibara Co· ，Ltd. , Okayama， Japan之產品）、0· 3重量份氫化澱粉、及0. 02重 量份由實施例A- 2方法所得之液體蜂膠萃取物混合。依 習知的方式，將所得之混合物塑型及包裝，可得所企求之 產物。 此產物爲一種日本點心，其由於含有蜂膠之有效成， 因而具有天然的氣味及味道，還有令人滿意之咀嚼性值、 較長之儲存期限及安定性。 (請先閱讀背面之注意事項再填寫本頁), TT 470749 A7 B7 Printed by Zhengong Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (19) v% ethanol aqueous solution (the pH of which has been adjusted to 7.8 with sodium carbonate) is fed into the column, The active ingredients of propolis are dissolved and extracted by countercurrent extraction. The pH of the extract was adjusted to 6.2 with a pH control agent and filtered to obtain a liquid propolis extract having an A 310 nin / A 6 0 0 nm absorption ratio of 6, 4 3 8 and improved water solubility. Its yield is about 30% (d. S.b. relative to the propolis starting material). As with the product of Example A-1, this product contains substance C with improved water solubility and has satisfactory stability, odor and taste. Therefore, it can be used arbitrarily or in combination with other substances for food, anti-susceptible medicines and cosmetics, as health food supplements, preservatives, antioxidants, immune control agents, macrophage activating factors, anti-tumor Agent or hair restorer. Examples A-4 According to the method of Example A-1, the ethanol concentration of the 8 0 v / v% ethanol aqueous solution containing the crude propolis extract prepared by the method of Example A_1 was reduced to 4 5 v / v% To separate into two phases, that is, the upper and lower layers, let the two layers stand for 5 hours at ambient temperature. Collect the upper layer and dilute it with the same amount of water, and feed the diluted solution into the filled macropores produced by AMBERLI TEX AD-7 " (Rohm & Haas Co. * Philadelphia, Pa., USA) Resin), washed with water, and fed with 95 v / v% ethanol aqueous solution to elute the effective ingredients of propolis. The resulting eluate was mixed with sodium bicarbonate to adjust PΗ to 6 _ 2 and get the A310nm / AetJOnm absorption ratio (please read the precautions on the back before filling this page), 1T- This paper size applies the Chinese National Standard (CNS) Α4 size (210x297 mm) -22-470749 Economy Printed by the Central Bureau of Standards of the People's Republic of China—Industrial and Consumer Cooperatives " A7 B7 V. Description of the Invention (20) 21, 892 and improved water-soluble liquid propolis extract. Its yield is about 22% (relative to the propolis starting material , D. S. B.). Same as the product of Example A-1, this product contains substance C with improved water solubility and has satisfactory stability, odor, and taste. Therefore, it can be arbitrarily or alone Other substance combinations for food, resistance to susceptible diseases Medicines and cosmetics, as health food supplements, preservatives, antioxidants, immune control agents, macrophage activating factor, antitumor agents or hair restoration agents. Example B-1 Gummy Candy under reduced pressure 100 parts by weight of "MA BIT®" (a kind of hydrogenated malt syrup, a product of Hayashibara Shoji, Inc. 'Okayama' Jap-an) and 50 parts by weight of "TREHA0SETM" (a high-purity, water-containing crystalline seaweed) Sugar, a product of Hayashibara Shoji, Inc. Okayama 'Japan) is heated and concentrated to become a concentrated body with a water content of about 15 w / w%. According to a conventional manner, the concentrate is made with 13 parts by weight of gelatin (dissolved in 18 parts by weight of water), 1 part by weight of the liquid propolis extract obtained by the method of Example A-1, and 2 parts by weight of citric acid. Sodium, and sufficient colorants and flavor enhancers. The resulting mixture is shaped and packaged as the desired product. "The product is a gummy that has a satisfactory taste and taste and does not induce tooth decay. This paper is in accordance with China National Standard (CNS) A4 specification (210X297 mm) -23- (Please read the precautions on the back before filling this page) Order 470749 A7 B7 Printed by the Central Standards Bureau of the Ministry of Economic Affairs and Consumer Cooperatives 2. Description of the invention (21) 眚 Example B-2 chewing gum is heated 3 parts by weight of the gum base until it melts, and the resulting substance and 4 parts by weight of sucrose and 3 parts by weight of "TREHAOSETM", 0.02 parts by weight The liquid propolis extract obtained by the method of Example A-1 was mixed with a sufficient amount of the colorant, and the mixture was kneaded with a roller, and then shaped and packaged to the desired product. This product is a chewing gum with satisfactory mouthfeel, odor and taste, which is less likely to cause tooth decay, because it contains an effective ingredient of propolis.眚 实施 例 B — 3 GV uhi (starch paste 1 so that 1 part by weight of glutinous rice starch is mixed with 1.2 parts by weight of water, and the resulting mixture is gelatinized by heating, and further with 1.5 parts by weight " "TREHA0SETM", 0.7 parts by weight of "SUNMALT®, containing crystal maltose (Hayashibara Co., Ltd., Okayama, Japan), 0.3 parts by weight of hydrogenated starch, and 0.02 parts by weight of Example A -The liquid propolis extract obtained in the method 2 is mixed. The obtained mixture is shaped and packaged in a conventional manner to obtain the desired product. This product is a Japanese confection, which contains propolis, and therefore has Natural odor and taste, as well as satisfactory chewing value, long shelf life and stability. (Please read the notes on the back before filling this page)

、tT 本紙张尺度適用中國國家標準（CNS ) A4規格（210X297公釐）-24 - 470749 經濟部中央標準局負工消费合作社印聚 A7 B7五、發明説明（22 ) 實施例B — 4 奶油飽(Cream filling) 依習知的方式，將1 〇 Ο 0重量份之” FINETOSE ® 、無水晶狀麥芽糖（Hayashibara Co. ，Ltd. ，Okayama ，Japan之產品）、2 〇 〇重量份無水晶狀海藻糖、 1 0 0 0重量份之使糕餅鬆脆的原料（shortening )、 5 0重量份可可塊、3重量份之由實施例A- 3之方法所 得液體蜂膠萃取物，以及1重量份卵磷脂混合，以獲得奶 油餡。 此產物由於含有蜂膠之有效成份，因此具有令人滿意 之安定性及儲存期限，且具有討喜之咀嚼性質及入口即.化 的性質。 實施例B — Η 奶油蛋糕 將50重量份之未加鹽的奶油、50重量份之使糕餅 鬆脆的材料（shortening)、5 0重量份蜂蜜、及1 3 0 重量份糖充分混合，且進一步與1 5 0重量份之蛋混合及 攙拌。令所得之混合物與135重量份麵粉、75重量份 牛奶、4重量份碳酸氫鈉及足量之香草調味劑混合。依習 知之方式，將如是所得之混合物倒入模具中，予以烘焙， 然後冷卻至周溫。將1 . 5重量份由實施例A- 1方法所 得液體蜂膠萃取物、20重量份日本杏酒、及20重量份 白蘭地酒混合，而製得糖漿，用刷子將糖漿塗在前述經烘 ^紙張尺度適用中國國家標準（CNS ) A4規格（21〇Χ297[釐）-25 - (請先閱讀背面之注意事項再填寫本頁) 470749 經濟部中央標率局員工消费合作社印製 A7 B7 五、發明説明（23) 焙之產品上，以獲得所企求之產品。 此產品具有令人滿意之氣味及味道且含有蜂膠之有效 成份，可隨意地用於維持及/或促進健康，以及預防及/ 或治療疾病，同時促進由疾病恢復健康。 實施例B — 6 冰淇淋 將2 3 0 0重量份之牛奶加熱至約6 0°C並於該溫度 下令其與200重量份蛋黃、50重量份蛋、420重量 份果糖、30重量份氫化澱粉、200重量份新鮮奶油、 20重量份蒸發過的牛奶、3重量份由實施例A—2方 法所得之液體蜂膠萃取物，以及1重量明膠粉混合。藉由 在7 5 °C下加熱，將所得之混合物殺菌，然後予以冷卻， 再於攪拌的條件下，與日本杏酒混合，將其倒入容器中且 予以冷凍，即可得所企求之產物。 該含有蜂膠之有效成份的產品可隨意地用於維持及/ 或促進健康，以及預防及/或治療疾病，同時促進由疾病 恢復健康。 眚施例B — 7 粉狀蜂膠萃取物 令5重量份由實施例A - 2方法所得液體蜂膠萃取物 與7重量份無水晶狀麥芽糖混合，且藉由加熱至4 0。(：之 空氣予以乾燥1小時，並進一步與3重量份無水晶狀海藻 ^紙张尺度適用中國國家標準（CNS ) A4规格（210X297公釐）_ 26 - ' (請先聞讀背面之注意事項再填寫本頁), TT This paper size is applicable to Chinese National Standard (CNS) A4 specification (210X297 mm) -24-470749 Central Consumers Bureau of the Ministry of Economic Affairs, Consumer Cooperative Cooperative Printing Poly A7 B7 V. Description of the invention (22) Example B-4 (Cream filling) 1000 parts by weight of "FINETOSE ®", crystal-free maltose (product of Hayashibara Co., Ltd., Okayama, Japan), 2000 parts by weight Sugar, 100 parts by weight of crisping ingredients (shortening), 50 parts by weight of cocoa mass, 3 parts by weight of the liquid propolis extract obtained by the method of Example A-3, and 1 part by weight of lecithin Mix to obtain a creamy filling. Because this product contains the effective ingredients of propolis, this product has satisfactory stability and shelf life, and has pleasing chewing properties and ready-to-eat properties. Example B — 奶油 Cream cake will 50 parts by weight of unsalted butter, 50 parts by weight of shorting for pastry, 50 parts by weight of honey, and 130 parts by weight of sugar are thoroughly mixed and further mixed with 150 parts by weight of eggs Mix and stir. Mix the resulting mixture with 135 parts by weight of flour, 75 parts by weight of milk, 4 parts by weight of sodium bicarbonate, and a sufficient amount of vanilla flavoring agent. Pour the resulting mixture into a mold in a conventional manner Bake and then cool to ambient temperature. 1.5 parts by weight of the liquid propolis extract obtained by the method of Example A-1, 20 parts by weight of Japanese apricot wine, and 20 parts by weight of brandy are mixed to prepare a syrup, using a brush Apply the syrup to the above-mentioned paper size of the bake paper ^ Applicable to China National Standard (CNS) A4 specification (21〇 × 297 [centimeters])-25-(Please read the precautions on the back before filling this page) 470749 Employees of the Central Standardization Bureau of the Ministry of Economic Affairs Printed by Consumer Cooperative A7 B7 V. Description of Invention (23) Baked products to obtain the desired product. This product has a satisfactory odor and taste and contains active ingredients of propolis, which can be used for maintenance and // Or promote health, and prevent and / or treat disease, while promoting recovery from disease. Example B-6. Ice cream heated 230 weight parts of milk to about 60 ° C and ordered it at that temperature With 200 parts by weight of egg yolk, 50 parts by weight of eggs, 420 parts by weight of fructose, 30 parts by weight of hydrogenated starch, 200 parts by weight of fresh cream, 20 parts by weight of evaporated milk, and 3 parts by weight of liquid propolis obtained by the method of Example A-2 The extract was mixed with 1 weight of gelatin powder. The resulting mixture was sterilized by heating at 75 ° C, then cooled, and then mixed with Japanese apricot wine under stirring, and poured into a container. And frozen, you can get the desired product. The product containing the effective ingredients of propolis can be optionally used for maintaining and / or promoting health, and preventing and / or treating diseases, while promoting recovery from diseases.眚 Example B-7 Powdery Propolis Extract 5 parts by weight of the liquid propolis extract obtained by the method of Example A-2 was mixed with 7 parts by weight of crystal-free maltose, and heated to 40. (: The air is dried for 1 hour, and further with 3 parts by weight of crystal-free seaweed ^ Paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) _ 26-'(Please read the precautions on the back first (Fill in this page again)

、tT 470749 A7 B7 經濟部中央標準局賀工消費合作社印製 五、發明説明（24 ) 糖及0. 05重量份碳酸氫鈉混合，以得粉狀蜂膠萃取物 »以1 g爲一份，將蜂膠萃取物分置於層壓鋁容器中。 由於此產物易於分散且溶於水，並具有令人滿意之味 道，因此可隨意地單獨用作爲健康食品、防腐劑、藥品、 增味劑、脫臭劑或尿療劑，且可作爲其他組成物之材料。 此產物亦可用於維持及/或促進健康，以及預防及/或治 療疾病，同時促進由疾病恢復健康。 當用作爲健康食品時，該產物可單獨使用或在以 0 . 2 — lg/200ml之量分散及溶於液體（諸如， 茶、牛奶或果汁）後使用。此產物亦可以0. 5 — 2g/ 1 0 〇m 1之量溶於新鮮尿液中，以爲尿療之用。 窗施例B _ 8 粉狀蜂膠萃取物 將2重量份由實施例A - 4方法所得之液體蜂膠萃取 物與2重量份r -環糊精及5重量份無水晶狀海藻糖混合 。藉由加熱至4 0 °C之空氣，將該混合物乾燥1小時，然 而令其與7重量份無水晶狀麥芽糖均勻混合，以得粉狀蜂 膠萃取物。以lg爲一份，將蜂膠萃取物分置於層壓鋁容 器中。 由於此產物易於分散且溶於水，並具有令人滿意之味 道，因此可隨意地單獨用作爲健康食品、防腐劑、藥品、 增味劑、脫臭劑或尿療劑，且可作爲其他組成物之材料。 此產物亦可用於維持及/或促進健康，以及預防及/或治 (請先閱讀背面之注意事項再填寫本頁), TT 470749 A7 B7 Printed by He Gong Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (24) Sugar and 0.05 parts by weight of sodium bicarbonate are mixed to obtain powdery propolis extract »1 g as a portion, The propolis extract was divided into laminated aluminum containers. Because this product is easily dispersed and soluble in water, and has a satisfactory taste, it can be used alone as a health food, preservative, medicine, flavor enhancer, deodorant or urinary therapy, and can be used as other components Material. This product can also be used to maintain and / or promote health, as well as prevent and / or treat diseases, while promoting recovery from diseases. When used as a health food, the product can be used alone or after dispersing and dissolving in a liquid such as tea, milk or fruit juice in an amount of 0.2-lg / 200ml. This product can also be dissolved in fresh urine in an amount of 0.5-2 g / 100 m 1 for the purpose of urine therapy. Window Example B_8 Powdery Propolis Extract 2 parts by weight of the liquid propolis extract obtained by the method of Examples A-4 were mixed with 2 parts by weight of r-cyclodextrin and 5 parts by weight of crystal-free trehalose. This mixture was dried by air heated to 40 ° C for 1 hour, and then it was uniformly mixed with 7 parts by weight of crystal-free maltose to obtain a powdered propolis extract. In a portion of lg, the propolis extract was dispensed into a laminated aluminum container. Because this product is easily dispersed and soluble in water, and has a satisfactory taste, it can be used alone as a health food, preservative, medicine, flavor enhancer, deodorant or urinary therapy, and can be used as other components Material. This product can also be used to maintain and / or promote health, and prevent and / or cure (please read the precautions on the back before filling this page)

,1T 本紙張尺度適用中國國家標準（CNS ) A4規格（21〇Χ297公釐）_ 27 470749 經濟部中央標準局員工消費合作社印" A7 B7 五、發明説明（25) 療疾病，同時促進由疾病恢復健康。 窗施例B - 9 配方飼料(Formula feed ) 將下列成份混合，以製得配方飼料：3 0重量份麥 麩粉、30重量份由實施例B-7方法所得粉狀蜂膠萃取 物、10重量份脫脂奶粉、10重量份含乳蔗糖之粉末、 10重量份綜合維生素、5重量份魚粉、5重量份二代磷 酸鈣、3重量份油脂、3重量份碳酸鈣2重量份鹽及2重 量份礦物質。 此產物除了呈現出蜂膠有效成份之活性外，還呈現出 令人滿意之促進比菲德菌（bifid bacteria)生長活性， 且其可隨意用於預防家禽之傳染病及痢疾，促進家禽之味 口及生長，且預防其排泄物有令人不悅的味道。視需要， 此產物可與其他的飼料用原料併用，例如，燕麥、麵粉、 澱粉、油渣粉、以及殘渣之濃縮物，以及粗食品原料，諸 如稻草、枯草、甘蔗渣、玉米穗軸及彼等之秣草。 窨施例B - 1 0 片劑 將3 0重量份由實施例B _ 7方法所得之粉狀蜂膠萃 取物、20 重量份” NYUKAOLIGO® L S -5 5P” （一種含有乳蔗糖的粉末，其係爲Hayashibar_a Shoji’ Inc. ’Okayama， Japan 之產品）、1〇 重量份 本紙張尺度適用中國國家標準（CNS)A4規格（ 210X297公釐）—28 _ (請先閱讀背面之注意事項再填寫本頁) 訂 " 470749 經濟部中央標準局員工消费合作社印製 Α7 Β7 五、發明説明（26) 乳酸鈣、5重量份L -抗壞血酸、5重量份支鏈澱粉、1 重量份α —糖基維生素P、〇· 2重量份碳酸鎂、1重 量份三代磷酸鈣、1重量份糖酯、以及足量之粉狀調味劑 均勻混合。用具有2 OR之沖孔力且直徑爲1 2 mm之製 片機，將混合物製成片劑。使用塗糖衣機，依習知的方法 ，將含有 50w/w%” TREHAOSE TM”及 〇 · 2 w/w%支鏈澱粉且已加熱至5 5 °C的水溶液塗在片劑上 ，並予以乾燥。重覆該塗覆步驟以獲得具有糖衣之片劑， 其具有含晶狀無水海藻糖之未加裝飾的表面。 一般而言，此一不會碎裂且具有令人滿意之安定性及 可吞食性的產物可以約1 一 4 0片/天/成人的劑量（較 佳爲約2-20片/天/成人的劑量），經口投與人類。 由於此產物具有蜂膠之有效成份且富含乳蔗糖及支鏈澱粉 ,其呈現出極強的腸控制活性。因此，此產物可令人滿意 地用作爲口服產物，以預防結腸癌。由於此產物富含鈣鹽 ，其利於作爲口服產物，以用於預防及/或治療骨質疏鬆 症，且由於此產物富含L_抗壞血酸及α-糖基維生素P ，其利於作爲口服產物，以促進健康及預防衰老。 實施例Β - 1 1 片劑 . 將1 0重量份由實施例Β - 8方法所得粉狀蜂膠萃取 物、70重量份海藻糖、62重量份玉米澱粉、以及8重 量份脂肪酸治糖酯混合均勻，且依實施例Β - 1 0之方法 本紙張尺度適用中國國家標隼（CNS ) Α4规格（210X297公釐）-29 - (請先閱讀背面之注意事項再填寫本頁) 、1Τ 470749 經濟部中央標準局貞工消费合作社印製 A7 B7 五、發明説明（27) 將混合物製成片劑。爲製得在胃中易於溶解的片劑，茲將 該片劑塗以糖依，先用5w/w%羥基丙基甲基纖維素預 塗覆該片劑，再用l〇w/w%羥丙基甲基纖維素予以塗 覆，以製得腸衣片劑（enteric — coated tablet)。 此等片劑利於作爲可供治療人類惡性腫瘤,，諸如，胃 癌、肺癌、肝細胞癌、子宮癌、乳癌、結腸癌及黑色瘤。 實施例B — 1 2 外用藥 將2 0重量份之含有實施例A - 4方法所得蜂膠有效 成份之上層及3 3重量份甲醇加至3 0 0重量份麥芽糖粉 及200重量份海藻糖中，且進一步添加200重量份之 1Ow/w%支鏈澱粉溶液，以製得具有適當之延伸性 及黏著性的創傷用軟膏。 此產物由於含有蜂膠之有效成份，因而呈現出令人滿 意之防腐性、抗發炎性、抗腫瘤性及局部麻醉活性，且由 於含有麥芽糖及海藻糖，而呈現出補充能量至活細胞的活 性。基於此等活性，該產物可縮短癒合時間且可令人滿意 地治療受傷的皮膚組織。 實施例B - 1 3 注射液 將由實施例A - 4方法所得液體蜂膠萃取物溶於3 0 v/v%乙醇水溶液中，且依習知的方法，對所得之混合 ;^紙张尺度適用中國國家標準（匚粑）八4規格（210\297公釐）-3〇- (請先閱讀背面之注意事項再填寫本頁), 1T This paper size applies the Chinese National Standard (CNS) A4 specification (21 × 297 mm) _ 27 470749 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs " A7 B7 V. Description of the invention (25) Treating diseases and promoting Disease recovers. Window Example B-9 Formula feed The following ingredients were mixed to prepare a formula feed: 30 parts by weight of wheat bran flour, 30 parts by weight of the powdered propolis extract obtained by the method of Example B-7, and 10 parts by weight. Parts of skimmed milk powder, 10 parts by weight of sucrose-containing powder, 10 parts by weight of multivitamins, 5 parts by weight of fish meal, 5 parts by weight of calcium secondary phosphate, 3 parts by weight of fat, 3 parts by weight of calcium carbonate, 2 parts by weight of salt, and 2 parts by weight Minerals. In addition to showing the activity of the active ingredients of propolis, this product also shows satisfactory growth promoting activity of bifid bacteria, and it can be used at will to prevent infectious diseases and dysentery of poultry and promote the taste of poultry. And grow, and prevent its excrement from having an unpleasant taste. If necessary, this product can be used in combination with other feed ingredients, such as oat, flour, starch, oil residue powder, and residue concentrates, as well as coarse food ingredients such as straw, hay, bagasse, corn cobs, and others Wait for the scribble.窨 Example B-10 Tablets 30 parts by weight of the powdered propolis extract obtained by the method of Example B _7, 20 parts by weight "NYUKAOLIGO® LS-5 5P" (a powder containing sucrose, which is a It is a product of Hayashibar_a Shoji 'Inc.' Okayama, Japan), 10 parts by weight This paper size applies to Chinese National Standard (CNS) A4 specification (210X297 mm) —28 _ (Please read the precautions on the back before filling this page ) Order 470749 Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs A7 B7 V. Description of the invention (26) Calcium lactate, 5 parts by weight of L-ascorbic acid, 5 parts by weight of amylopectin, 1 part by weight of α-glucosyl vitamin P , 0.2 parts by weight of magnesium carbonate, 1 part by weight of calcium tertiary phosphate, 1 part by weight of sugar ester, and a sufficient amount of powdery flavoring agent are uniformly mixed. The mixture was made into tablets using a tablet machine having a punching force of 2 OR and a diameter of 12 mm. Using a sugar coating machine, according to a conventional method, an aqueous solution containing 50 w / w% "TREHAOSE TM" and 0.2 w / w% amylopectin and heated to 55 ° C was coated on the tablets and applied dry. This coating step was repeated to obtain a sugar-coated tablet having an undecorated surface containing crystalline anhydrous trehalose. Generally speaking, this product that does not crack and has satisfactory stability and swallowability can be dosed at about 1 to 40 tablets / day / adult (preferably about 2-20 tablets / day / adult Dose), administered orally to humans. Because this product has the effective ingredients of propolis and is rich in sucrose and amylopectin, it exhibits strong intestinal control activity. Therefore, this product can be satisfactorily used as an oral product to prevent colon cancer. Since this product is rich in calcium salts, it is beneficial as an oral product for the prevention and / or treatment of osteoporosis, and because this product is rich in L_ascorbic acid and α-glycosyl vitamin P, it is beneficial as an oral product to Promote health and prevent aging. Example B-1 1 tablet. 10 parts by weight of the powdered propolis extract obtained by the method of Example B-8, 70 parts by weight of trehalose, 62 parts by weight of corn starch, and 8 parts by weight of fatty acid sugar-reducing ester were mixed uniformly. According to the method of Example B-10, the paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X297mm) -29-(Please read the precautions on the back before filling this page), 1T 470749 Ministry of Economic Affairs Printed by the Central Standards Bureau Zhengong Consumer Cooperative A7 B7 V. Description of Invention (27) The mixture is made into tablets. In order to obtain a tablet that is easy to dissolve in the stomach, the tablet is coated with tang, the tablet is pre-coated with 5w / w% hydroxypropyl methylcellulose, and then 10w / w% Hydroxypropyl methylcellulose was coated to prepare an enteric-coated tablet. These tablets are useful for treating human malignancies such as gastric cancer, lung cancer, hepatocellular carcinoma, uterine cancer, breast cancer, colon cancer and melanoma. Example B — 1 2 For external use, 20 parts by weight of the upper layer containing the active ingredient of propolis obtained in the method of Examples A-4 and 33 parts by weight of methanol were added to 300 parts by weight of maltose powder and 200 parts by weight of trehalose. Furthermore, 200 parts by weight of a 10 w / w% amylopectin solution was further added to prepare a wound ointment having appropriate stretchability and adhesiveness. This product contains the effective ingredients of propolis, so it exhibits satisfactory antiseptic, anti-inflammatory, antitumor and local anesthetic activities, and because it contains maltose and trehalose, it shows the activity of supplementing energy to living cells. Based on these activities, the product can shorten the healing time and can satisfactorily treat injured skin tissue. Example B-1 3 Injection Solution The liquid propolis extract obtained by the method of Example A-4 was dissolved in a 30 v / v% ethanol aqueous solution, and the obtained mixture was mixed according to a known method; ^ Paper size is applicable to China National Standard (匚 粑) 8 4 Specifications (210 \ 297 mm) -3〇- (Please read the precautions on the back before filling this page)

、tT " 470749 A7 B7 五、發明説明（28) 物進行膜過濾，以獲得不含熱原之溶液，然後予以分裝於 2 Om 1之小瓶中，以得5mg/小瓶之蜂膠萃取物（ d . s . b )，予以冷凍乾燥且密封，即得所企求的產物 〇 此產物可單獨使用或在經肌下或靜脈投服至人類之前 與其他維生素及/或礦物質合併。此產物可隨意地用於促 進疾病的治療，諸如，免疫疾病、循環疾病、神經疾病及 惡性腫瘤，且可用於自此等疾病恢復健康。 實施例B — 1 4 注射液 將6重量份氯化鈉、0 . 3重量份氯化鉀、0 . 2重 量份氯化鈣、3 _ 1重量份乳酸鈣、4 5重量份海藻糖以 及0.1重量份由實施例A-4方法所得液體蜂膠萃取物 溶於1 0 0 0重量份之2 v / v %乙醇水溶液中。依習知 之方法，對所得之溶液進行膜過濾，以製得不含熱原的溶 液，以25ml爲一份，將所得溶液分置於塑膠容器中， 以獲得所企求之產物。 此產物可作爲維生素P、能量或礦物質補充劑，以促 進疾病的治療及促進自疾病恢復健康。 眚施例B —丄_5__ 捅管餵食劑. 將2 0重量份無水海藻糖、1 . 1重量份甘胺酸、 __本紙张尺度適用中國國家標準（CNS ) A4規格（210X297公釐）-31- (請先閲讀背面之注意事項再填寫本頁), TT " 470749 A7 B7 V. Description of the invention (28) The membrane is filtered to obtain a pyrogen-free solution, and then aliquoted into a vial of 2 Om 1 to obtain a 5 mg / vial of propolis extract ( d. s. b), freeze-dried and sealed to obtain the desired product. This product can be used alone or combined with other vitamins and / or minerals before being administered to humans under the muscle or intravenously. This product is optionally used to promote the treatment of diseases such as immune diseases, circulatory diseases, neurological diseases and malignant tumors, and can be used to restore health from these diseases. Example B — 1 4 An injection solution includes 6 parts by weight of sodium chloride, 0.3 parts by weight of potassium chloride, 0.2 parts by weight of calcium chloride, 3-1 parts by weight of calcium lactate, 45 parts by weight of trehalose, and 0.1. A part by weight of the liquid propolis extract obtained by the method of Example A-4 was dissolved in a 2 v / v% ethanol aqueous solution of 100 parts by weight. According to a conventional method, the obtained solution is subjected to membrane filtration to obtain a pyrogen-free solution, and 25 ml is used as a portion, and the obtained solution is divided into plastic containers to obtain a desired product. This product can be used as a vitamin P, energy or mineral supplement to promote the treatment of disease and promote the recovery from disease.眚 例 B — 丄 _5__ 捅 tube feeding agent. 20 parts by weight of anhydrous trehalose, 1.1 parts by weight of glycine, __ This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) -31- (Please read the notes on the back before filling this page)

X 經濟部中央標準局員工消费合作社印製 經 濟 部 中 央 標 準 消 費 合 社 印 製 470749 A7 B7 __ 五、發明説明（29 ) 0.18重量份穀胺酸鈉、1· 2重量份鹽、1重量份檸 檬酸鈉、0. 4重量份乳酸鈣、〇·1重量份碳酸鎂、 0. 1重量份由實施例B- 7方法所得粉狀蜂膠萃取物、 0. 0 1重量份維生素31及0. 0 1重量份維生素B2 混合，以製得一組成物。以2 4 g爲一整份，，將該組成物 分置於層壓鋁小袋中，並將彼等小袋密封，即得插管餵食 劑。 此產物可用作爲補充劑，經口投服或在將一袋之產物 溶於約300 — 500ml水後，藉由插管餵食，非經腸 地投服至鼻孔、胃或腸。 實施例B — 1 6 浴鹽 令2 1重量份之乳酸DL —鈉、8重量份丙酮酸鈉、 3重量份海藻糖、5重量份由實施例a-3方法所得液體 蜂勝萃取物，以及3 7重量份乙醇與2 6重量份純水混合 ，且添加足量之色料及芳香劑，以獲得浴鹽。 此產物可在浴缸中，以熱水予以溶解，稀釋10 0-1 0 0 0倍’以作爲皮膚細緻劑或皮膚增白劑。此產物亦 可在溶於清潔液或乳液後使用。 實施例B — 1 7 乳液 依習知方法進行加熱’以溶解〇 . 5重量份聚氧乙烯 ik张尺度適用中國國家標準（CNS ) A4規格---- △漫^— 3 2 - I----------'------1T------沒—— (請先閱讀背面之注意事項再填寫本頁) 470749 A7 B7 經濟部中央標準局只工消费合作社印裝 五、發明説明（3〇 ) 二十二醚、1重量份單硬脂酸甘油酯、〇· 5重量份丙酮 酸、0. 5重量份二十二醇、1重量份鱷梨油、1重量份 由實施例A - 2方法所得液體蜂膠萃取物、以及足量維生 素P及一防腐劑。令所得之混合物與〇 . 5重量份L —乳 酸鈉、0. 5重量份海藻糖、5重量份1， 3-丁二醇、 0.1重量份羧基乙烯基聚合物以及84. 3重量份純水 混合，且藉由均化機予以乳化。令所得之乳液與足量之芳 香劑混合，即得乳液。 此產物可作爲防曬劑、皮膚細緻劑或皮膚增白劑。 實施例B — 1 8 乳膏 將2重量份聚氧乙二醇硬脂酸酯、5重量份自乳化性 硬脂酸甘油酯、2重量份之由實在A - 3方法所得液體蜂 膠萃取物、1重量份液態石蠘、10重量份三辛酸甘油酯 以及足量防腐劑混合。藉加熱溶解該混合物，然後，令其 與2重量份海藻糖、5重量份1， 3_丁二醇以及66重 量份純水混合，接著藉由均化器來乳化該混合物，且令其 與足量芳香劑混合，即可得乳膏。 此產物可作爲防曬劑、皮膚細緻劑或皮膚增白劑。 眚施例B — 1 9 洗髮精 藉加熱，將1. 0重量份由實施例A-4方法所得液 _^^尺度適用中國國家標準（匚奶）八4規格（2丨0'乂297公釐）_33_ 一 (請先閱讀背面之注意事項再填寫本頁) 訂 470749 經濟部中央標準局貝工消费合作社印製 A7 B7 五、發明説明（31) 體蜂膠萃取物、0. 2重量份烷基二胺基甘胺酸氫氯酸鹽 溶液、20. 0重量份月桂基二甲基胺基乙酸甜菜鹼、 25. 0重量份月桂醯基甲基牛磺化物（tauride )，以及5 2 . 0重量份純水、足量防腐劑及芳香劑溶解 ，以獲得洗髮精。 由於此產物具有令人滿意之頭法清洗能力且較不會傷 害頭髮，因此其可隨意地用於促進動物（包括人類）之毛 髮生長，以及預防及/或治療頭皮屑、頭皮癢及掉髮。 眚施例B - 2 0 濶絲精 藉由加熱，溶解2. 5重量份由實施例A—1方法所 得液體蜂膠萃取物、2. 0重量份氯化二硬脂基二甲基銨 、2 . 0重量份十六烷醇、2 . 0重量份矽酮油、1 . 0 重量份聚氧乙烯油基醇醚，以及足量芳香劑。於攪拌的條 件下，令該混合物與由3. 0重量份1, 3 — 丁二醇、 89. 0重量份純水及足量防腐劑所組成之混合物一起攪 拌，並將結果所得之混合物冷卻且令其靜置，即可得潤絲 稩。 此產物可有效地潤洗頭髮且可隨意地用於促進動物（ 包括人類）之毛髮生長，以及預防及/或治療頭皮屑、頭 皮癢及掉髮。 實施例B — 2 1 本&張尺度適用中國國家標準（CNS ) A4規格（210X297公釐）_ 34 _ -----一------. .------II------R- (請先閱讀背面之注意事項再填寫本頁) 470749 經濟部中央標準局員工消费合作社印製 A7 ______B7五、發明説明（32 ) 毛髮滋養劑 將2重量份” a GRUTIN” （一種α—糖基維生素p， Toyo Sugar Refining Co. ，Ltd. ， Tokyo » Japan 的 產品），以及2 0 · 〇重量份甘油混合且溶於5 5 〇重量 份之已加熱至6 0 °C的純水中，且令此混合物與藉由下列 方法所得溶液：將0 _ 〇 5重量份” KANK0S0 3 0 1 (碘 化6— [2 -[( 5 —溴基一 2 —吡啶基）胺基]乙烯基]— 1-乙基一2-皮考啉）”（一種花青顏料，Photosen- sitizing Dyes Co. ’ Ltd· ，Okayama’ Japan 的產品 )、以及10重量份由實施例A - 2方法所得液體蜂膠萃 取物溶於440重量份乙醇中；以及藉由將2. 0重量份 1 -苯烷醇溶於1 0重量份乙醇所製得之溶液混合。將所 得之混合物過濾且裝罐，即得頭髮滋養劑。 此產物可有效地促進毛髮生長且治療及/或預防禿髮 及掉髮，且其可令人滿意地用於預防頭皮屑及頭皮癢並可 作爲創傷之藥物，因其具有令人滿意之防腐性及抗炎活性 〇 實施例B - 2 2 髮液 令55. 0重量份乙醇、20. 0重量份聚氧丙烯（ 40) 丁基醚以及13. 0重量份純水與1. 0重量份由 實施例A—1方法所得液體蜂膠萃取物及3. 0重量份2 一 ◦ — a — D —吡喃葡糖基—L 一抗壞血酸混合，以製得 (請先閱讀背面之注意事項再填寫本頁) 本紙张尺度適用中國國家標準（CNS ) Α4規格（210Χ297公釐）-35 - 470749 經濟部中央標準局賀工消费合作社印製 Α7 Β7 五、發明説明（33) 一組成物。依習知的方式，令該混合物與足量的pH -控 制劑、芳香劑及防腐劑混合，即可得髮液。 此產物可有效地促進毛髮生長且治療及/或預防禿髮 及掉髮，且其可令人滿意地用於預防頭皮屑及頭皮癢並可 作爲創傷之藥物，因其具有令人滿意之防腐性及抗炎活性 〇 實施例B - 2 3 髮乳 將3. 0重量份蜂蠟、15. 0重量份凡士林、 42. 0重量份液態石蠟、3. 0重量份聚氧乙烯（5) 硬脂酸酯、2. 0重量份聚氧乙烯（6)油基醇醚、 1_ 0重量份聚氧乙燦（6)細獵基醇酸、1. 0重量份 由實施例A-3方法所得液體蜂膠萃取物、〇. 3重量份 2 — 〇 — a — D —吡喃葡糖基—L —抗壞血酸及3 2重量 份純水混合，以製得組成物。依習知的方式，令該混合物 與足量的ρ Η -控制劑、芳香劑及防腐劑混合，即可得髮 乳。 此產物可用於促進毛髮生長及治療及/或預防禿髮、 掉髮、頭皮屑及頭皮癢。 實施例Β — 2 4 牙膏 製備由下列成份所組成的牙膏。此產物具有防腐性， 本紙張尺度適用中國國家標準（CNS ) Α4規格（210X297公釐）—36 _ (請先閲讀背面之注意事項再填寫本頁)X Printed by the Consumer Standards Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs Printed by the Central Standards Consumer Cooperative of the Ministry of Economic Affairs 470749 A7 B7 __ V. Description of the Invention (29) 0.18 parts by weight of sodium glutamine, 1.2 parts by weight of salt, 1 part by weight of lemon Sodium sodium, 0.4 parts by weight of calcium lactate, 0.1 parts by weight of magnesium carbonate, 0.1 parts by weight of the powdered propolis extract obtained by the method of Example B-7, 0.01 parts by weight of vitamin 31 and 0.0 1 part by weight of vitamin B2 was mixed to prepare a composition. Taking 2 4 g as a whole, this composition was divided into laminated aluminum pouches, and their pouches were sealed to obtain an intubation feeding agent. This product can be used as a supplement, taken orally or after dissolving a bag of the product in about 300-500ml of water, feeding through a cannula, and parenterally to the nostrils, stomach or intestines. Example B—16 bath salts made 21 parts by weight of lactic acid DL-sodium, 8 parts by weight of sodium pyruvate, 3 parts by weight of trehalose, 5 parts by weight of the liquid bee extract obtained by the method of Example a-3, and 37 parts by weight of ethanol is mixed with 26 parts by weight of pure water, and sufficient amounts of colorants and fragrances are added to obtain bath salts. This product can be dissolved in hot water in a bathtub and diluted 10 to 0-1000 times' as a skin refiner or skin whitener. This product can also be used after dissolving in a cleaning solution or emulsion. Example B — 1 7 Emulsion was heated according to a conventional method to dissolve 0.5 parts by weight of polyoxyethylene ik sheet scale applicable to China National Standard (CNS) A4 specifications ---- △ ^-3 2-I- --------'------ 1T ------ No—— (Please read the notes on the back before filling this page) 470749 A7 B7 Central Standards Bureau, Ministry of Economic Affairs Printed 5. Description of the invention (30) dodecyl ether, 1 part by weight glyceryl monostearate, 0.5 part by weight pyruvate, 0.5 part by weight eicosanediol, 1 part by weight avocado oil, 1 part by weight of the liquid propolis extract obtained by the method of Example A-2, and a sufficient amount of vitamin P and a preservative. The resulting mixture was mixed with 0.5 parts by weight of L-sodium lactate, 0.5 parts by weight of trehalose, 5 parts by weight of 1,3-butanediol, 0.1 parts by weight of a carboxyvinyl polymer, and 84.3 parts by weight of pure water. And emulsified by a homogenizer. The obtained emulsion is mixed with a sufficient amount of fragrance to obtain an emulsion. This product can be used as a sunscreen, skin refiner or skin whitener. Example B — 1 8 The cream contains 2 parts by weight of a polyoxyethylene stearate, 5 parts by weight of a self-emulsifiable glyceryl stearate, 2 parts by weight of a liquid propolis extract obtained by the actual method A-3, 1 part by weight of liquid ocher, 10 parts by weight of trioctanoic acid glyceride and a sufficient amount of a preservative are mixed. The mixture was dissolved by heating, then, it was mixed with 2 parts by weight of trehalose, 5 parts by weight of 1,3-butanediol, and 66 parts by weight of pure water, and then the mixture was emulsified by a homogenizer, and mixed with Mix a sufficient amount of fragrance to get a cream. This product can be used as a sunscreen, skin refiner or skin whitener.眚 例 B—1 9 By heating the shampoo, 1.0 part by weight of the liquid obtained by the method of Example A-4 _ ^^ scales apply the Chinese National Standard (匚 奶) 8 4 specifications (2 丨 0 '乂 297 Mm) _33_ First (please read the precautions on the back before filling this page) Order 470749 Printed by the Central Bureau of Standards of the Ministry of Economic Affairs and printed by the Shellfish Consumer Cooperative A7 B7 V. Description of the invention (31) Body propolis extract, 0.2 parts by weight Alkyl diaminoglycine hydrochloride solution, 20.0 parts by weight lauryldimethylaminoacetic acid betaine, 25.0 parts by weight laurylmethyltauride, and 5 2 0 parts by weight of pure water, sufficient preservatives and fragrances are dissolved to obtain shampoo. Since this product has satisfactory scalp cleaning ability and is less harmful to hair, it can be used at will to promote hair growth in animals (including humans) and prevent and / or treat dandruff, itchy scalp and hair loss .眚 例 例 B-2 濶 丝 精 By heating, dissolve 2.5 parts by weight of the liquid propolis extract obtained by the method of Example A-1, 2.0 parts by weight of distearyldimethylammonium chloride, 2 0 parts by weight of cetyl alcohol, 2.0 parts by weight of silicone oil, 1.0 part by weight of polyoxyethylene oleyl alcohol ether, and a sufficient amount of fragrance. Under stirring conditions, the mixture is stirred with a mixture consisting of 3.0 parts by weight of 1,3-butanediol, 89.0 parts by weight of pure water and a sufficient amount of a preservative, and the resulting mixture is cooled. And let it stand still, you can get moisturizing silk. This product is effective in rinsing hair and can optionally be used to promote hair growth in animals, including humans, and to prevent and / or treat dandruff, itchy scalp and hair loss. Example B — 2 1 This & Zhang scale is applicable to the Chinese National Standard (CNS) A4 specification (210X297 mm) _ 34 _ ----- one ------. .------ II- ----- R- (Please read the notes on the back before filling this page) 470749 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 ______B7 V. Invention Description (32) Hair nourishing agent will be 2 parts by weight "a GRUTIN "(An α-glycosyl vitamin p, a product of Toyo Sugar Refining Co., Ltd., Tokyo» Japan), and 20 · 〇 parts by weight of glycerin are mixed and dissolved in 5 500 parts by weight have been heated to 60 ° C in pure water, and this mixture and the solution obtained by the following method: 0 _ 05 parts by weight "KANK0S0 3 0 1 (iodinated 6-[2-[(5-bromo-2-pyridyl) Amine] vinyl] —1-ethyl-2-picolin) (a cyanine pigment, Photosen-Sizing Dyes Co. 'Ltd., a product of Okayama' Japan), and 10 parts by weight of Example A -2 liquid propolis extract obtained by the method 2 is dissolved in 440 parts by weight of ethanol; and a solution prepared by dissolving 2.0 parts by weight of 1-benzanol in 10 parts by weight of ethanol Mixed. The resulting mixture was filtered and canned to obtain a hair tonic. This product can effectively promote hair growth and treat and / or prevent baldness and hair loss, and it can be satisfactorily used to prevent dandruff and itchy scalp and can be used as a trauma drug because it has a satisfactory antiseptic Sexual and anti-inflammatory activity. Example B-2 2 The hair fluid makes 55.0 parts by weight of ethanol, 20.0 parts by weight of polyoxypropylene (40) butyl ether, and 13.0 parts by weight of pure water and 1.0 parts by weight. The liquid propolis extract obtained by the method of Example A-1 and 3.0 parts by weight 2-a-D-glucopyranosyl-L-ascorbic acid were mixed to obtain (Please read the precautions on the back before filling (This page) This paper is in accordance with the Chinese National Standard (CNS) A4 specification (210 × 297 mm) -35-470749. Printed by the Hegong Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs. Α7 Β7 5. Description of the invention (33) A composition. In a conventional manner, the mixture is mixed with a sufficient amount of a pH-controlling agent, a fragrance and a preservative to obtain a hair liquid. This product can effectively promote hair growth and treat and / or prevent baldness and hair loss, and it can be satisfactorily used to prevent dandruff and itchy scalp and can be used as a trauma drug because it has a satisfactory antiseptic Sexual and anti-inflammatory activity Example B-2 3 Hair cream will be 3.0 parts by weight beeswax, 15.0 parts by weight vaseline, 42.0 parts by weight liquid paraffin, 3.0 parts by weight polyoxyethylene (5) stearin Acid ester, 2.0 parts by weight of polyoxyethylene (6) oleyl alcohol ether, 1_ 0 parts by weight of polyoxyethylene (6) fine hunting alkyd, 1.0 part by weight of the liquid obtained by the method of Example A-3 Propolis extract, 0.3 parts by weight of 2--0-a-D-glucopyranosyl-L-ascorbic acid and 32 parts by weight of pure water were mixed to obtain a composition. By conventionally mixing the mixture with a sufficient amount of ρρ-controlling agent, fragrance and preservative, a hair cream is obtained. This product can be used to promote hair growth and treat and / or prevent baldness, hair loss, dandruff and itchy scalp. Example B-2 4 Toothpaste A toothpaste consisting of the following ingredients was prepared. This product is anti-corrosive, and the paper size applies to Chinese National Standard (CNS) Α4 specification (210X297 mm) —36 _ (Please read the precautions on the back before filling this page)

、tT 470749 Μ Β7五、發明説明（34) 可有效地用於預防口臭及蛀牙。 組成物 二代磷酸鈣 支鏈澱粉 月桂基硫酸鈉 甘油 聚氧乙烯山梨糖醇酐月桂酸酯 防腐劑 4 重量份 2 . 9 5.重量份 重量份 2 0 . 0重量份 0 . 5重量份 0 . 0 5重量份 實施例A—3方法所得液體蜂膠萃取物 12. 0重量份 (請先閱讀背面之注意事項再填寫本頁) 經濟部中央標率局Μ工消费合作社印裂 麥芽糖醇 0重量份 水 0重量份 如前所述，本發明係關於具有水溶性改良之蜂膠萃取 本紙張尺度適用中國國家標準（CNS ) A4規格（210X297公釐）_ 37 一 470749 A7 _B7 五、發明説明（35) 物有效成份（尤指物質C)的蜂膠萃取物，且提供易於呈 現蜂膠有效成份之活性且具有令人滿意之安定性、氣味及 味道的蜂膠萃取物。 本發明之蜂膠萃取物不侷限用於習知之高度刺激性且 易溶於水之有機溶劑的高濃度溶液中，且其亦可自由地用 於前述溶劑的低濃度溶液中。因此，本發明之蜂膠萃取物 可隨意地用於諸如食品、抗易感受性疾病的藥劑以及化粧 品等組成物中。 本發明之蜂膠萃取物具有大於4，0 0 0之A310rim /Ae6〇nm吸收比，且具有令人滿意的顏色、氣 味及味道，對於食品界、化粧品界及藥品界有深不可測的 影響。 雖然本文以敘述了本發明之較佳體系，但是，習於此 藝之士應可瞭解可對彼等體係作各種修改，且本發明之申 請專利範圍係涵蓋所有如是之符合本發明精髓且落入本發 明範圍的修改。 (請先閲讀背面之注意事項再填寫本頁) 訂 經濟部中央標率局員工消费合作社印掣 祕 釐 公 97 2, TT 470749 Μ B7 V. Description of the invention (34) can be effectively used to prevent bad breath and tooth decay. Composition Calcium Phosphate Phosphate Pullulan Sodium Lauryl Sulfate Glycerin Polyoxyethylene Sorbate Laurate Preservative 4 parts by weight 2. 9 5. parts by weight 20.0 parts by weight 0.5 parts by weight 0 0 5 parts by weight of the liquid propolis extract obtained in the method of Example A-3, 12. 0 parts by weight (please read the precautions on the back before filling this page). Part of water 0 parts by weight As mentioned above, the present invention is about propolis extraction with improved water solubility. The paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) _ 37 470749 A7 _B7 V. Description of the invention (35 Propolis extracts (especially substance C), and provide propolis extracts that are easy to exhibit the activity of active ingredients of propolis and have satisfactory stability, odor and taste. The propolis extract of the present invention is not limited to conventional high-concentration solutions that are highly irritating and easily soluble in water, and it can also be used freely in low-concentration solutions of the aforementioned solvents. Therefore, the propolis extract of the present invention can be optionally used in compositions such as foods, anti-susceptible agents, and cosmetics. The propolis extract of the present invention has an absorption ratio of A310rim / Ae60nm greater than 4,000, and has satisfactory color, flavor and taste, and has profound and unpredictable effects on the food, cosmetics and pharmaceutical industries. Although this article describes the preferred system of the present invention, those skilled in the art should understand that various modifications can be made to their systems, and the scope of patent application of the present invention covers all such cases that meet the essence of the present invention and fall into Modifications that fall within the scope of the invention. (Please read the precautions on the back before filling out this page.) Order Printed by the Consumer Standards Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 97 2

Claims (1)

90.470749 Α8 Β8 C8 D8 經濟部智慧財產局員工消費合作社印製· 七、申請專利範圍 附件A :第8 6 1 〇 3 8 3 4號專利申請案 中文申請專利範圍修正本 民國9 0年9月修正 1. 一種供製備蜂膠萃取物的方法，該萃取物具有 5. 5至7. 0之pH值，藉以改良蜂膠之有效成份的水 溶性’且含有3 — [4 -羥基—3，5_二（3-甲基一 2 _ 丁烯基）苯基]- 2 -丙烯酸爲該蜂膠之一有效成份 ’當溶於約0 . 5 v/v%乙醇水溶液中時，含量在2 5 。(：下爲至少0_ 45ppm，當其在3 1 Onm及660 nm之吸收轉化爲在2w/w%濃度（基於乾固體）之蜂 膠萃取物的吸收時，其具有A3i〇nm/A66t)nm大於 4，0 0 0的吸收比， 此方法包含（i )令蜂膠與p Η未經控制之易溶於水 有機溶劑的水溶液接觸，以浸漬萃取法或逆流萃取法溶解 並萃取蜂膠之有效成份，利用ρ Η控制劑將蜂膠萃取物之 pH調整爲5. 5 — 7_ 0並收集蜂膠萃取物；或是 (ii)令蜂膠與pH已調爲5. 5或更高之易溶於水有 機溶劑的水溶液接觸，以溶解並萃取蜂膠之有效成份，以 及視情況需要，在收集萃取物之前，用ρ Η控制劑將蜂膠 萃取物之pH調整爲5. 5 — 7. 0，最後收集 ρΗ5_5 — 7_0之蜂膠萃取物， 其中該易溶於水之有機溶劑係選自丙酮、乙酸、甲醇 、乙醇、異丙醇以及彼等之混合物，其中該ρ Η控制劑係 (請先閱讀背面之注意事項再填寫本頁) 訂 線 本紙張尺度適用中國國家揉準（CNS ) Α4規格（210X297公兼） 一 1 - 470749 A8 B8 C8 D8 六、申請專利範園 選自氧化鎂、氧化鈣、氫氧化鈉、氫氧化鉀、氫氧化鎂、 氫氧化鈣、碳酸氫鈉、碳酸氫鉀、碳酸鈉、碳酸鎂以及彼 等之混合物。 2. —種蜂膠萃取物，其係由如申請專利範圍第1項 的方法製得，且該萃取物具有5. 5至7. 0之pH值， 藉以改良蜂膠之有效成份的水溶性，且含有3 - [ 4 -羥 基—3， 5_二（3 —甲基—2- 丁烯基）苯基]_2 — 丙烯酸爲該蜂膠之一有效成份，當溶於約〇. 5v/v% 乙醇水溶液中時，含量在25 °C下爲至少〇. 4 5 ppm ’當其在3 1 0 nm及6 6 0 nm之吸收轉化爲在2 w/w%濃度（基於乾固體）之蜂膠萃取物的吸收時，其 具有八31。11111/八66。11„1大於4，0 0 0的吸收比。 3. 如申請專利範圍第2項之蜂膠萃取物，其係用於 食品、化粧品或藥品之中。 (請先閱讀背面之注意事項再填寫本頁) Γ 經濟部智慧財產局員工消費合作社印製 本紙張尺度適用中國國家橾準（CNS ) A4規格（210X297公釐） -2 -90.470749 Α8 Β8 C8 D8 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs. VII. Patent Application Scope Annex A: No. 8 6 1 〇3 8 3 No. 4 Chinese Patent Application Amendment Amendment of the Republic of China September 1990 1. A method for preparing a propolis extract, the extract having a pH value of 5.5 to 7.0, thereby improving the water solubility of the effective ingredients of propolis' and containing 3-[4-hydroxy-3, 5_ two (3-Methyl-2_butenyl) phenyl] -2-acrylic acid is one of the effective ingredients of this propolis. When dissolved in about 0.5 v / v% ethanol aqueous solution, the content is 25. (: At least 0_45ppm, when its absorption at 3 1 Onm and 660 nm is converted into absorption of propolis extract at a concentration of 2w / w% (based on dry solids), it has A3onm / A66t) nm greater than The absorption ratio of 4, 0, 0, this method includes (i) contacting propolis with an uncontrolled aqueous solution of pΗ which is easily soluble in water and organic solvents, and dissolving and extracting the effective components of propolis by immersion extraction method or counter current extraction method, Use ρ Η control agent to adjust the pH of the propolis extract to 5.5-7_ 0 and collect the propolis extract; or (ii) make the propolis and pH have been adjusted to 5.5 or higher easily soluble in organic solvents The aqueous solution is contacted to dissolve and extract the active ingredients of propolis, and if necessary, before collecting the extract, adjust the pH of the propolis extract to 5. 5 — 7. 0 with ρ Η control agent, and finally collect ρΗ 5_5 — 7_0 Propolis extract, wherein the water-soluble organic solvent is selected from the group consisting of acetone, acetic acid, methanol, ethanol, isopropanol, and mixtures thereof, and the ρ Η control agent (please read the precautions on the back first) (Fill in this page) China National Standard (CNS) A4 specification (210X297) and 1-470749 A8 B8 C8 D8 6. The patent application park is selected from magnesium oxide, calcium oxide, sodium hydroxide, potassium hydroxide, magnesium hydroxide, hydroxide Calcium, sodium bicarbonate, potassium bicarbonate, sodium carbonate, magnesium carbonate and mixtures thereof. 2. A kind of propolis extract, which is prepared by the method as described in the first patent application range, and the extract has a pH value of 5.5 to 7.0, so as to improve the water solubility of the effective ingredients of propolis, and Contains 3-[4-hydroxy-3, 5-di (3-methyl-2-butenyl) phenyl] _2 — acrylic acid as an active ingredient of the propolis, when dissolved in about 0.5v / v% ethanol In aqueous solution, the content is at least 0.4 5 ppm at 25 ° C. When its absorption at 3 1 0 nm and 6 6 0 nm is converted to a propolis extract at a concentration of 2 w / w% (based on dry solids) When it is absorbed, it has an absorption ratio of 8 31.11111 / 8 66. 11 „1 is greater than 4, 0 0 0. 3. For example, the propolis extract of item 2 of the patent scope is used in food, cosmetics or medicine (Please read the notes on the back before filling out this page) Γ The paper size printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs applies to the Chinese National Standard (CNS) A4 specification (210X297 mm) -2-
TW86103834A 1995-11-24 1997-03-26 Propolis extract with improved water-solubility TW470749B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116763999A (en) * 2023-08-25 2023-09-19 四川大学 Urinary system catheter using propolis alcohol extract as coating and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116763999A (en) * 2023-08-25 2023-09-19 四川大学 Urinary system catheter using propolis alcohol extract as coating and preparation method thereof
CN116763999B (en) * 2023-08-25 2023-11-07 四川大学 Urinary system catheter using propolis alcohol extract as coating and preparation method thereof

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