TW289800B - Differential confocal microscopy - Google Patents
Differential confocal microscopyInfo
- Publication number
- TW289800B TW289800B TW84110928A TW84110928A TW289800B TW 289800 B TW289800 B TW 289800B TW 84110928 A TW84110928 A TW 84110928A TW 84110928 A TW84110928 A TW 84110928A TW 289800 B TW289800 B TW 289800B
- Authority
- TW
- Taiwan
- Prior art keywords
- technique
- confocal microscopy
- resolution
- curve
- depth resolution
- Prior art date
Links
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- Microscoopes, Condenser (AREA)
Abstract
By utilizing the sharp slope of the axial response curve of confocal imaging, we invented a differential confocal technique with depth resolution much greater than conventional confocal microscopy. The axial response curve of confocal microscopy is approximately a sinc2 curve, and the depth resolution is the full width at half maximum of the curve. In contrast to conventional confocal microscopy, our technique works at the sharp slope of the response curve, sample displacement causes sensitive differential change of the signal, hence great depth resolution can be obtained. The lateral resolution is limited by the diffraction limit of light, and the depth resolution is only limited by noise in the system. Combined with scanning mechanisms, this technique can be used to measure surface profiles and display their three dimensional images with great resolution. The technique does not require locking sample position by servo feedback loop, therefore it has real-time imaging capability.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW84110928A TW289800B (en) | 1994-10-17 | 1994-10-17 | Differential confocal microscopy |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW84110928A TW289800B (en) | 1994-10-17 | 1994-10-17 | Differential confocal microscopy |
Publications (1)
Publication Number | Publication Date |
---|---|
TW289800B true TW289800B (en) | 1996-11-01 |
Family
ID=51398191
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TW84110928A TW289800B (en) | 1994-10-17 | 1994-10-17 | Differential confocal microscopy |
Country Status (1)
Country | Link |
---|---|
TW (1) | TW289800B (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107765425A (en) * | 2017-06-30 | 2018-03-06 | 长春理工大学 | Self-focusing laser scanning projection method based on symmetrical defocus double detector |
CN108286936A (en) * | 2017-04-18 | 2018-07-17 | 北京理工大学 | Laser micro/nano processes differential confocal on-line monitoring integral method and device |
CN109520973A (en) * | 2018-11-13 | 2019-03-26 | 北京理工大学 | Postposition is divided pupil laser differential confocal microscopic detection method and device |
-
1994
- 1994-10-17 TW TW84110928A patent/TW289800B/en not_active IP Right Cessation
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108286936A (en) * | 2017-04-18 | 2018-07-17 | 北京理工大学 | Laser micro/nano processes differential confocal on-line monitoring integral method and device |
CN107765425A (en) * | 2017-06-30 | 2018-03-06 | 长春理工大学 | Self-focusing laser scanning projection method based on symmetrical defocus double detector |
CN107765425B (en) * | 2017-06-30 | 2019-11-19 | 长春理工大学 | Self-focusing laser scanning projection method based on symmetrical defocus double detector |
CN109520973A (en) * | 2018-11-13 | 2019-03-26 | 北京理工大学 | Postposition is divided pupil laser differential confocal microscopic detection method and device |
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