TW289800B - Differential confocal microscopy - Google Patents

Differential confocal microscopy

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Publication number
TW289800B
TW289800B TW84110928A TW84110928A TW289800B TW 289800 B TW289800 B TW 289800B TW 84110928 A TW84110928 A TW 84110928A TW 84110928 A TW84110928 A TW 84110928A TW 289800 B TW289800 B TW 289800B
Authority
TW
Taiwan
Prior art keywords
technique
confocal microscopy
resolution
curve
depth resolution
Prior art date
Application number
TW84110928A
Other languages
Chinese (zh)
Inventor
Jyh-Pyng Uang
Chau-Hwang Lii
Original Assignee
Jyh-Pyng Uang
Chau-Hwang Lii
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jyh-Pyng Uang, Chau-Hwang Lii filed Critical Jyh-Pyng Uang
Priority to TW84110928A priority Critical patent/TW289800B/en
Application granted granted Critical
Publication of TW289800B publication Critical patent/TW289800B/en

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Abstract

By utilizing the sharp slope of the axial response curve of confocal imaging, we invented a differential confocal technique with depth resolution much greater than conventional confocal microscopy. The axial response curve of confocal microscopy is approximately a sinc2 curve, and the depth resolution is the full width at half maximum of the curve. In contrast to conventional confocal microscopy, our technique works at the sharp slope of the response curve, sample displacement causes sensitive differential change of the signal, hence great depth resolution can be obtained. The lateral resolution is limited by the diffraction limit of light, and the depth resolution is only limited by noise in the system. Combined with scanning mechanisms, this technique can be used to measure surface profiles and display their three dimensional images with great resolution. The technique does not require locking sample position by servo feedback loop, therefore it has real-time imaging capability.
TW84110928A 1994-10-17 1994-10-17 Differential confocal microscopy TW289800B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW84110928A TW289800B (en) 1994-10-17 1994-10-17 Differential confocal microscopy

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW84110928A TW289800B (en) 1994-10-17 1994-10-17 Differential confocal microscopy

Publications (1)

Publication Number Publication Date
TW289800B true TW289800B (en) 1996-11-01

Family

ID=51398191

Family Applications (1)

Application Number Title Priority Date Filing Date
TW84110928A TW289800B (en) 1994-10-17 1994-10-17 Differential confocal microscopy

Country Status (1)

Country Link
TW (1) TW289800B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107765425A (en) * 2017-06-30 2018-03-06 长春理工大学 Self-focusing laser scanning projection method based on symmetrical defocus double detector
CN108286936A (en) * 2017-04-18 2018-07-17 北京理工大学 Laser micro/nano processes differential confocal on-line monitoring integral method and device
CN109520973A (en) * 2018-11-13 2019-03-26 北京理工大学 Postposition is divided pupil laser differential confocal microscopic detection method and device

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108286936A (en) * 2017-04-18 2018-07-17 北京理工大学 Laser micro/nano processes differential confocal on-line monitoring integral method and device
CN107765425A (en) * 2017-06-30 2018-03-06 长春理工大学 Self-focusing laser scanning projection method based on symmetrical defocus double detector
CN107765425B (en) * 2017-06-30 2019-11-19 长春理工大学 Self-focusing laser scanning projection method based on symmetrical defocus double detector
CN109520973A (en) * 2018-11-13 2019-03-26 北京理工大学 Postposition is divided pupil laser differential confocal microscopic detection method and device

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