TW266264B - Quick genotyping of porcine stress syndrome from blood samples - Google Patents

Quick genotyping of porcine stress syndrome from blood samples

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Publication number
TW266264B
TW266264B TW82109851A TW82109851A TW266264B TW 266264 B TW266264 B TW 266264B TW 82109851 A TW82109851 A TW 82109851A TW 82109851 A TW82109851 A TW 82109851A TW 266264 B TW266264 B TW 266264B
Authority
TW
Taiwan
Prior art keywords
pss
dna
mutation
blood
stress syndrome
Prior art date
Application number
TW82109851A
Other languages
Chinese (zh)
Inventor
Yih-Chian Jeng
Ming-Yeun Liou
Original Assignee
Pig Res Inst Taiwan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pig Res Inst Taiwan filed Critical Pig Res Inst Taiwan
Priority to TW82109851A priority Critical patent/TW266264B/en
Application granted granted Critical
Publication of TW266264B publication Critical patent/TW266264B/en

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Abstract

Porcine Stress Syndrome (PSS) is responsible for the sudden death of pigs subjected to stress, which is induced by the mutation of ryanodine receptor gene of skeletal muscle. In this invention, we use DNA either from heparined blood or blood dried out on a filter as template, and a pair of oligonucleotide primers specifically designed based on the knowledge of the nucleotide sequences of ryanodine receptor gene. A small region of DNA arrounding the PSS mutation was amplified by using polymerase chain reaction (PCR). The DNA fragments were treated with restriction enzymes, and the genotype was determined by agarose gel electrophoresis. This DNA-based test can clearly distinguish the genotypes of homozygous PSS, homozygous normal, and heterozygous pigs, and therefore could be used to create an improved lean, heavily-muscled but no PSS swine breed.
TW82109851A 1993-11-23 1993-11-23 Quick genotyping of porcine stress syndrome from blood samples TW266264B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW82109851A TW266264B (en) 1993-11-23 1993-11-23 Quick genotyping of porcine stress syndrome from blood samples

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW82109851A TW266264B (en) 1993-11-23 1993-11-23 Quick genotyping of porcine stress syndrome from blood samples

Publications (1)

Publication Number Publication Date
TW266264B true TW266264B (en) 1995-12-21

Family

ID=51402172

Family Applications (1)

Application Number Title Priority Date Filing Date
TW82109851A TW266264B (en) 1993-11-23 1993-11-23 Quick genotyping of porcine stress syndrome from blood samples

Country Status (1)

Country Link
TW (1) TW266264B (en)

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