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Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Abstract
Porcine Stress Syndrome (PSS) is responsible for the sudden death of pigs subjected to stress, which is induced by the mutation of ryanodine receptor gene of skeletal muscle. In this invention, we use DNA either from heparined blood or blood dried out on a filter as template, and a pair of oligonucleotide primers specifically designed based on the knowledge of the nucleotide sequences of ryanodine receptor gene. A small region of DNA arrounding the PSS mutation was amplified by using polymerase chain reaction (PCR). The DNA fragments were treated with restriction enzymes, and the genotype was determined by agarose gel electrophoresis. This DNA-based test can clearly distinguish the genotypes of homozygous PSS, homozygous normal, and heterozygous pigs, and therefore could be used to create an improved lean, heavily-muscled but no PSS swine breed.
TW82109851A1993-11-231993-11-23Quick genotyping of porcine stress syndrome from blood samples
TW266264B
(en)
Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing