經濟部屮央標準局貝工消"合作社印製 209853 Λ6 ___Η 6 _ 五、發明説明（1 ) 本發明之技術領域 本發明傺關於一種用於撖置溶液連接型的轉注及處理条統，而能夠有效率且曲绩的進 行徹量樣本溶液的轉注及/或處理。 先前技蓊的概要説明 傳統上,在解析生物化學及臨床化學領域中的研究一般均偽以毫升量的樣本處理溶液 為工作基礎。近來由於生物科技及免疫化學上的發展，在這些領域的趼究係根據處理樣 本溶液其量小到撤升所得之結果。當樣本溶液的處理單位變得很小時，下述問題即發生。 在利用高性能液相層析，高性能毛細管Zone electrophoresis或許多其他技術來分 析生物樣本時，經常必須在分析前，對樣本做先期處理。在其他情形下，必須接纊進行兩 個或以上的酵素消化作用以得到冀求的産品。在這些狀況中，該樣本溶液(樣本溶液係經 由酵素反應在反應管中所獲得)必須經過超過濾膜的過濾，以除去有箸很大分子重量的分 子或著不溶的細粒子，從而防止阻塞住高性能液相層析柱。 一般而言，一種設備(例如微滴液管)用來將樣本溶液從反應管轉注入另一裝置中以進 行超過濾。但是在此種方法下，在轉注樣本溶液的過程中不可避免的會損失一定數量的 樣本。此種損失在樣本數量變小時會更大。 在另一例子中，可用放射性同位素來標示一種蛋白質，然後標示的蛋白質成分及同位素 應分離。在此種狀況，傳統上在反應管中用同位素標示後，部分或所有的樣本溶液被徹滴 液管或類似物轉注於一種潮定輻射性的装置中。因此，上述的損失樣本的問題也在轉注 樣本溶液的過程中會發生&同時，用於液體轉注的裝置不可避免的會有輻射感染的危險 Ο 再者，當執行樣本操作這種原本即需要多數步驟的程序時(例如上述之酵素反應及樣本 放射性同位素標示程序），因為這些樣本操作程序必須轉注該樣本許多次,所以樣本損失 的置以及裝置受感染的程度就變得越來越鹾重。 本發明的概要説明 本發明包括一連接型處理条統及方法，以用於徹量溶液的轉注，包括(1)第一容器(來源 或反應管)有著管狀外形，其第一端開啓而相對的第二端關閉，在管中産生樣本溶液的反 應；（2)第二容器（標的管）與第一容器外形相固.¾笛一端顒铉而—端醐閉：好（幻一tel 本紙張尺度逡用中國明家«準(CNS)IM規格(2丨0x297公；it) _ 3 _ 81. 4. 1 (請先閲讀背而之注意事項#璜寫本頁) 209853 Λ 6 Π 6209853 Λ6 ___ Η 6 _ printed by the Cooperative Society of the Central Standards Bureau of the Ministry of Economic Affairs V. Description of the invention (1) The technical field of the present invention The present invention relates to a transfer and treatment system for the connection of the solution, In addition, the injection and / or processing of the sample solution can be efficiently and efficiently performed. A summary of previous techniques. Traditionally, research in the fields of analytical biochemistry and clinical chemistry has generally assumed a milliliter sample processing solution as the basis of work. Due to recent developments in biotechnology and immunochemistry, research in these fields is based on the results of processing sample solutions whose amount is as small as withdrawal. When the processing unit of the sample solution becomes very small, the following problems occur. When using high-performance liquid chromatography, high-performance capillary zone electrophoresis or many other techniques to analyze biological samples, it is often necessary to pre-process the samples before analysis. In other cases, two or more enzymes must be digested to obtain the desired product. In these situations, the sample solution (the sample solution is obtained in the reaction tube via an enzyme reaction) must be filtered through an ultrafiltration membrane to remove molecules with a large molecular weight or insoluble fine particles, thereby preventing clogging Live high performance liquid chromatography column. In general, a device (e.g., a microdropper) is used to transfer the sample solution from the reaction tube into another device for ultrafiltration. However, under this method, a certain number of samples will inevitably be lost during the transfer of sample solution. This loss will be greater when the sample size becomes smaller. In another example, a radioisotope can be used to label a protein, and then the labeled protein components and isotopes should be separated. In this situation, after traditionally labeled with isotopes in the reaction tube, part or all of the sample solution is transferred to a tidal radiation device by a dropper or the like. Therefore, the above-mentioned problem of sample loss also occurs during the transfer of the sample solution. At the same time, the device used for liquid transfer will inevitably have the risk of radiation infection. Furthermore, when the sample operation is performed, it is necessary. Most of the procedures (such as the above-mentioned enzyme reaction and sample radioisotope labeling procedures), because these sample operation procedures must be transferred to the sample many times, so the loss of the sample and the degree of infection of the device become more and more important . SUMMARY OF THE INVENTION The present invention includes a connection-type processing system and method for the transfer of a full amount of solution, including (1) the first container (source or reaction tube) has a tubular shape, the first end of which is open and opposite The second end of the valve is closed, and a reaction of the sample solution is generated in the tube; (2) The second container (standard tube) is solid with the shape of the first container. ¾ The flute is blunt at one end and closed at the end: good The paper standard is based on the Chinese Mingjia standard (CNS) IM specification (2 丨 0x297 g; it) _ 3 _ 81. 4. 1 (please read the back-end notes # 络 write this page) 209853 Λ 6 Π 6
經濟部中央楳堆扃员工消伢合作社印$L 五、發明説明（2) 連接器總成，用來連接第一容器的開口端及第二容器的開口端，也用於在施加預定的處理 的同時將樣本溶液從第一容器(來源管)轉移到第二容器(標的管)。該連接器總成包括一 個在箸中央穿孔的連接器構件,以容纳有著超過濾膜的一個膜支架。一掴栓塞位於膜支 架中以定位該膜。在另一實施例中，膜支架偽與連接器一體成形。 依據本發明的另一方面，偽使用連接型處理条統於撤量溶液的一種處理撤量溶液的方 法，其步驟包括執行在第一容器内的樣本溶液反應，用連接器绝成來連接第二容器的開口 端與第一容器的開口端，將已連接的第一及第二容器整値翻轉，並使用連接器總成施加預 定的處理且同時將樣本溶液從第一容器轉移到第二容器。至少有一個螺旋蓋子用來密封 來源管或標的管之一或兩個都密封。 本發明可以使樣本溶液在容器之間轉注的同時使用兩個容器及一個特別設計的連接器 總成(該總成用於連接這些容器)對該溶液施加預定的處理。因此，我們就不需要用轉注 裝置(例如徹滴液管），而且樣本損失的問題及感染的危險即可大幅減少或減至最小。 附圖之概要說明 圖1是一個用於撤量溶液的連接型轉注及處理条統及方法的示意圖。 圖2是部分剖面圖，顯示依據本發明第一實施例所建構的離心連接型撤量溶液轉注及 處理装置的特定結構。 圖3a-3b是一糸列圖顯示圖2中所示的管子10的結構。 圖4a-4b是一糸列圖顯示圖2中雙管連接器16的結構。 圖5是-条列四値部分圖顯示圖2中過濾元件支承構件22的結構。 圖6a-6b是一条列圖,顯示圖2中栓塞34的結構。 圖7是部分剖面圖，顯示離心連接型徹置溶液轉注及處理条統,該条統偽依本發明第 二實施例所建構。 圖8是部分剖面圖，顯示圖7中撤量溶液轉注/處理装置第二實施例中的一個管子， 並有一螺旋蓋子122。 圖9是一横剖面分解圖,顯示圖7中微量溶液轉注/處理裝置第二實施例，並省略了 上部或來源管ll〇b。 圖10是頂端視圖,顯示圖7中撤量溶液處理裝置第二實施例的栓塞150,其偽沿箸 (請先閲讀背而之注意事項#搆寫本頁) 裝· 訂- 線· 本紙张尺度逍用中國國家榣準(CNS)甲4規怙(210X297公龙)_ 4 81. 4. 10,000張(H) 209853 Λ 6 Π 6 經濟部t央標準局貝工消赀合作社印5i 五、發明説明（3 ) 圖9的10-10線及箭頭的方向所得到的。 圖11是圖7中第二實施例裝置的栓塞150的等角圖。 圖11a是一透視圖顯示工具162,該162傜用來將栓塞150插入連接器126的内 圓筒130c 圖12是第二實施例微量溶液處理裝置的連接器126的頂端圖,顯示連接器的膜支承 部分。 圖13是第二實施例撤量溶液處理裝置的連器的膜支承部分的横剖面分解圖，其偽沿 著圖12的13-13線及箭頭方向所得到的。 圖14是接頭170的側視圖，該170像用來將本發明撤置溶液處理裝置第二實施例 固定於一個離心轉子中。 圖15是圖14接頭170的横剖面的側視圖。 画16是一個等角圖，顯示微量溶液處理裝置第二實施例如何裝入接頭中(以横剖面顯 示)。 圖17是機能示意圖的部分横剖面,顯示本發明徹量溶液處理裝置第二賁施例被接頭 所固定，並且位於固定角度的轉子中。 本發明的詳細說明 以下的詳細内容偽以例子的方式說明了本發明，而並非限制了本發明的原刖。本詳細 說明使得熟諳本技藝之人可清楚地製造及使用本發明，並且解釋了本發明的數個實施例 及不同之態樣以及本發明的用途，包括申請人目前認為是本發明最佳之態樣。 圖1偽示意圖,説明了本發明連接型徹量溶液轉注及處理糸統及方法的整體条統原則 及方法步驟。本發明目前之較佳實施例偽關於一種處理方法及条統，以使用超濾膜於高 * 性能液相層析，從而對溶液做先期處理。 參照圖1(a),—研究員首先執行一種預定之化學反應，例如酵素反應,於容器或試管 A中。所得出的溶液或産品傺以圖1的虛線來表示。一個蓋子(未顯示)可用於試管的 開口端。 · 其次，如圖1(b)所示，在反應結束時，實驗者即從試管A除去蓋子(未顯示)並接合連接 器C的一端於試管A的開口。第二容器以圖中的試管B代表,其有箸與試管A相同之外 形，並且以倒轉的方式接合於連接器C的另一端。連接器C包括其中的-個超濾膜(未顯 (請先Mift背而之注意事項再填寫本頁) 裝- 訂- 線< 本紙張尺度逍用中國困家標毕(CNS)甲4規格(210X297公; 81. 4. 10,000張(H) 209853 Λ 6η 6 經濟部中央標準局貞工消ft·合作杜印製 五、發明説明（4 ) 示)〇 其次，如圖1(c)所示，該處理条統一體形成管Α及Β,且連接器C是倒轉'(如交鐮的箭 頭所示），並插入離心的分離器D中，然後旋轉該離心分離器。在此例子中，管A稱為”來 源管”或”反應管”,且管B稱為”標的管”。 因為離心作用的關係（見圖1(d)),在反應(來源)管A中的樣本溶液穿過在連接器C 中的超濾膜而進入標的管B。去除了溶劑的分子而仍有箸預定或較大的分子重量的，就 被超濾膜所陷住。 如上述，依本發明的數實施例,離心作用係在含有樣本溶液的反寒管及用於離心處理的 管子與有著超濾膜的連接器一體接合時開始進行。由於不需要使用一艏撤滴液管在來源 管及標的管之間轉注溶液，所以不會因為在微滴液管裝置中有殘餘的溶液而造成溶液的 損失。同時也避免了滴液管可能受到的污染。再者，與溶液係由"直接灌注法”來轉注 相tb (此種方法偽將反應(來源)管中的物品灌注於標的管中），由於離心過濾的完全性，所 以本發明不會在内來源管壁造成樣本溶液殘留。 當以多數步驟執行一反應時，在上述圖l(a)-(d)中的處理可在第二步驟後的各步驟 重覆進行，亦即使用管B (原先是標的管而現在有著過濾後的溶液，見圖1(d))來做為新 的反應(來源)管A 並且加入新的標的管B ’等等。 圖2是依本發明第一實施例建構的徹量溶液轉注/處理糸統裝置的部分剖面圖。徹置 溶液處理糸統裝置1傷成連接狀態，與圖1 (c)及(d)的示意圖一致。 撤量溶液處理糸統裝置1包括反應或來源管10a及標的管10b,其各有一開口端 12a, 12b相對，並由一連接器總成16所結合。管l〇a, l〇b外形相同且最好是由經常 用於徹離心應用的塑膠材料,例如聚丙烯或聚乙烯所製造，管l〇a, l〇b分別與圖1的 管A及B—致，且連接器總成16與圖1的連接器C一致。 連接器總成16包括連接器構件17,膜支架22,及一個拴塞34。連接器構件或連接 器17有著兩個不同的連接器端以用來與管l〇a, l〇b的管開口 12a, 12b相接合，此 兩連接器端包括第一連接器端18,其偽一個開口式的構件並有箸漸縮的承受内壁19以 與相對應的管開口 12a或12b的外緣壁14a, 14b形成適切的阽合；另外有第二連接 器端20,其有著一個公螺旋部分19沿箸其外緣壁設置，以與相對應的管開口 12a, 12b 的内緣壁上的母螺旋部分15a, 15b相接合。在圖2中,連接器17有著第一連接器端 (請先閲讀背而之注意事項再填寫本頁) 裝· 訂- 線. 本紙》尺度遑用中國Η家«準(CNS)T4規格(210X297公；《；) 6 81. 4. 10,000張（Η) Λ 6 Π6 09853 五、發明説明（5 ) 18與來源管i〇a的管開口 i2a的外緣壁Ua貼合，且第二連接器端20的母螺旋部 分19與檫的管10b的管開口 12b的内母螺旋部分15b成螺紋接合。 (請先閲讀背而之注意事項再蜞寫本頁) 膜支架22有一公螺旋部分24沿著一外緣壁形成，其螺紋的尺寸係可承接管開口 12a, 12b的内緣壁母螺旋部分15a. 15b的螺紋。在此例子中，膜支架22的外緣壁公 螺旋部分24與來源管開口 12a的內緣壁母螺旋部分15a相接合。膜支架22係調整 來承接一個沿著底支撑表面設置的超濾膜(見圖5 )。一個栓塞34偽用於確保該膜固 定於膜支架22中。 圖3是兩値放大的圖，顯示管1〇的細部構造。在此，管1〇可以是來源管l〇a或標 的管10b。圖3中的(a)部分是管10的開口 12的平面圖，且0>)部分是一剖面圖，顯 示管開口 12的平坦外緣壁14及母螺旋部分的内緣壁15。管開口 12的壁厚度” t ” 最好是向箸其自由端稍撤漸縮，以使連接器構件17的承接連接器端18能夠容易地插 入。 圖4是放大的兩圖,顯示圖2的連接器17的結構，其中(a)部分是平面圖，而(b)部 分是横剖面圖。連接器17的横剖面大致是圓形且包括一値内止擋表面或壁架19,使得 条統裝置1完全接合時(見圖2),管開口 12及膜支架24的端部抵住19而被限制在 一種鄰接的狀態。連接器17有一中央孔23,使得溶液可由第一管轉注於相連的第二管 中。 圖5是放大的一条列四個圖,顯示圖2的膜支架構件22的結構，其中（a)部分是頂 視圖(支撑表面26省略）；(b)部分是一横剖面圖；（c)部分是一側視圖:且(d)部分一放大 的底視圖，顯示形成於底壁26 (見a部分)的多數穿孔或導管28的構造。但是為了清 楚起見，導管28並未在(b)部分横剖面圖中顯示。 經濟部中央標準·局κζ工消$：；合作社印!52. 圖6是一条列兩艏圖,顯示圖2的圓形栓塞34的結構，其中(a)部分是一侧視圖，且 * (b)部分是一頂視圖。栓塞34像一値環或圓形構件,並在其外緣壁38上有一環嬈肋 36,其像用來配合插入膜支架22的内綠壁29上的相應槽27 (見圖5)。 兩痼管子l〇a及10b的組合如上述係可同時達成有效率的轉注溶液及離心處理(見 圖 1)。 . 圖7-13顯示本發明撤量溶液轉注處理糸統裝置的第二實施例，其係以圖中的構件 81. 4. 10,〇〇〇張'(H) 本紙張尺度逍用中國國家櫺準(CNS)IM規格(210X297公龙）_ 7 209853 Λ 6 Π 6 經濟部屮央標準Λ,员工消炸合作社印製 五、發明説明（6 ) 100來代表。參照圖7,微量溶液處理条統裝置的第二實施例100包括兩掴類似外形的 容器或管子110a, 110b,其各有一開口端112a, 112b,它們在使用時偽由一連接器總成 126所接合。第二的實施例的連接器總成126包括兩個主要元件，亦即一個連接器/過 據器扣件127及一個栓塞150。 如圖7及圖9,連接器構件127傜雙環槽結構，其有一外緒國柱殼部分或套筒128 圍繞著一個内圖筒部分130並由一徑向延伸的横腹板132所一體接合。外殼(套筒） 128及内圓筒形成兩個連接器端，包括第一螺紋連接器端136及第二滑接連接器端140。 外殼部分或套筒128最好是在137處呈鋸齒狀或滾花狀,以協肋使用者的操作。類似 的協肋握持表面120a, 120b可設於管子110a, 110b的外表面。 在本例子中,螺紋連接器端136包括母螺紋沿著外圓柱部分128的内緣壁設置，以接 合管ll〇a的管開口 112a的外緣壁設置的公螺紋114a。再者，滑接連接器端140貼 合於標的管ll〇b的開口端112b及公螺紋114b。連接器127的圓筒部分130也包 括一値横向膜支架表面或區域134。使用時，連接器構件127接合於管開口，使得膜支 架的内圓筒130偽接合於標的管110b的管開口 112b中。内圓筒130的膜支架表面 134形成了一個孔板，而超濾膜156即位於該板上。超濾膜156俗被一個栓塞150所 穩固地定位,其偽在使用時接合於内圓筒130中。 管形栓塞150及内圓筒130的壁的較佳高度是在離心作用時能夠確保所有的溶篏均 能保留在由管形栓塞150的孔所形成的圓柱容量中,使得代表溶液損失的彎月面不會超 過栓塞150或圓筒130之上。該容量一般在徹量溶液工作上是介於500徹升至600 徹升。另外，栓塞150的壁高度最好是稍微低於内圓筒130的圍繞壁部分的高度，使得 拴塞150向内漸縮端158形成漸進的過渡，以在離心作用時促進液體從來源管向下全 部流入標的管中。另外，形成内圓筒130的開口的端壁最好在166處(見圖9)去掉一 點角,以更促進液體全部流下進入内圓筒130。 圖8顳示單一管110,其有箸一個螺旋頂蓋122以用來旋上管開口 112的外公螺紋 114。蓋子122包括一値Ο形環124以確保液體不會損失。蓋122上的螺紋對密封來 源管ll〇a很有用(例如在酵素反應發生後），或者在已得到微量溶液所冀求的處理後密 封標的管之用。 參照圖9-11,栓塞150包括多數的凹口部分沿著頂緣壁154以相等距離間隔設置。 (請先閲讀背而之注意事項再填寫本頁) 裝< 訂- 線- f紙张尺度逍用中Β ΒΓ#·楳準(CNS)Ή規怙(210x297公；Jt) _ 8 81. 4. 10,000ft (H) 209853 Λ 6η 6 經濟部屮央標孕扃只工消许合作社印製 五、發明説明（7) 道些凹口部分160協助栓塞插入接合於連接器總成126的内圓筒膜支架130中。栓 塞150最好是包括一艏長形槽(未顯示)沿著其外囫柱壁形成，以協助空氣交換並從而在 拴塞150接合於内圓筒膜支架130中時，排洩出任何陷在内圓筒膜支架130及栓塞 150中的空氣。圖11a顯示一種用於將栓塞150插入内圖筒130中的示範工具162 。工具162最好包括軸向延伸的緣凸出構件164,以接合管形栓塞150的凹口部分 160〇 栓塞150的頂緣154最好是在158處漸縮，以確保所有的撤Λ溶液在使用時向箸超 濾膜流動,且不會在高於栓塞緣154處被陷住。同樣的，凹口 160所有的邊緣的外形最 好是圓的，以促進並確保液髖的流動。 圖12及13顯示連接器127的内圓筒130的類似孔板的膜支架區域134的細節。 該多孔板區域134包括多數的弓形及半弓形穿孔或導管142被肋部分144所間隔。 在孔板134膜支架區域的外緣包括了一個稍徹隆起的肋構件146,其有箸一頂峰在栓塞 150插入内圓筒130中時，與管形拴塞150的低端壁152協調對齊。參照圖13即可 知(栓塞150及膜152偽以虛影代表）。如此一來，膜156即能保持繃緊而防止因為底 端壁152與隆起肋構件146相接合而移開。 _ 14-16顯示接頭170可用來將第一或第二實施例撤置溶液轉注/處理糸統100接 合於離心轉子的承接套中。因為微量溶液處理系統更多的連接構件可增加環繞的周長， 所以徹置溶液處理糸統比起傳統的離心管有著稍撤長一點的外半徑。因此，最好在離心 轉子中有一直徑較寬的套,以承接該雙管/連接器糸統。為了此目的，一個接頭170用來 確保該徹量溶液条統100穩固阽切地接合於離心轉子中。接頭170横剖面是圓柱形， 並且其内徑的尺寸僳可在連接型撤量溶液条統插入其中時確保密切的公差接合。接頭 170的外表面有一横向延伸的環繞壁架構件174 (—値環形凸緣），其傺在插入接合於離 * 心轉子的承接套176中時,做為止檔構件及扶架支撑。 圖17顯示条統裝置100位於接頭170中，並且插入於轉子Γ78的適當承接套或孔 176中。該接頭包括在其底端的一値較小半徑的開口 180,其尺寸僳可在沿著底管鄰接 於連接器總成的位置與撤量溶液糸統100的管子之一的外部接合，從而条統装置100 . 的底端182不致於接觸離心轉子178的底部184或側壁185。接頭170高於壁架構 件174的直立壁186的長度足以確保在離心作用時對於連接型徹量溶液處理条統裝置 (請先閲讀背而之注意事項孙填寫本頁) 裝· ···#- 線· 本紙张尺度遑用中國國家樣毕(CNS)T4規格(210x297公*) _ 9 81. 4. 10,000張(H) Λ 6 It 6 209853 五、發明説明（8 ) 有適切的支撑。 圖16顯示接頭的前部可在188處切掉(以虚線表示)，從而只留下高於琿形凸緣或壁 架構件174的上接頭壁的高背支撑部分。（切除部分以構件171來代表)如此一來就造 成了一種輕重量的接頭而仍有足夠的支撑以減輕離心力所加諸於条統裝置的壓力。 雖然上述的實施例偽關於高性能液體層析的先期處理条統，其中一個超濾膜俗位於連 接器部分，在本發明另一實施例中，一種蓮用親合性的先期處理条統可藉由在連接中提供 親合性機能膜而執行。例如，連接器膜可含有抗體或抗原及外源凝集素或一個離子交換 膜，或一値有其他適當功能的膜。 雖然本發明已經詳細解釋及說明了，但是我們應瞭解在本行業有普通技蕤之人應可在 本發明意旨範圍内做各種修改而不會偏離本發明之精神。因此本發明之意旨參照前述說 明係由下列申請專利範圍所界定。 (請先閲讀背而之注意事項再塡寫本頁) 經濟部屮央標準局员工消作合作社印^ 81. 10,000張(H) 本紙張尺度逡用中BB家《毕(CNS)T4規格(210x297公«：> _Printed by the Ministry of Economic Affairs of the Central People ’s Republic of China Cooperative Society $ L. V. Description of the invention (2) Connector assembly, used to connect the open end of the first container and the open end of the second container, also used to apply predetermined treatments Simultaneously transfer the sample solution from the first container (source tube) to the second container (target tube). The connector assembly includes a connector member that is perforated at the center of the chopstick to accommodate a membrane holder with an ultrafiltration membrane. A slap plug is located in the membrane holder to position the membrane. In another embodiment, the membrane bracket is pseudo-integrated with the connector. According to another aspect of the present invention, a method for processing a withdrawal solution using a connection-type processing strip in combination with a withdrawal solution includes the steps of performing a reaction of a sample solution in a first container and connecting the first solution with a connector The open end of the two containers and the open end of the first container, turn over the connected first and second containers, and use the connector assembly to apply a predetermined process and at the same time transfer the sample solution from the first container to the second container. At least one screw cap is used to seal one or both of the source and target tubes. The present invention enables the sample solution to be transferred between the containers while using two containers and a specially designed connector assembly (the assembly is used to connect these containers) to apply a predetermined treatment to the solution. Therefore, we do not need to use a transfusion device (such as a dropper), and the problem of sample loss and the risk of infection can be greatly reduced or minimized. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram of a connected transfer injection and treatment system and method for withdrawal of solution. Fig. 2 is a partial cross-sectional view showing a specific structure of a centrifugal connection type withdrawal solution transfer and processing device constructed according to the first embodiment of the present invention. 3a-3b are a series of diagrams showing the structure of the tube 10 shown in FIG. 4a-4b are diagrams showing the structure of the double-tube connector 16 in FIG. FIG. 5 is a four-column partial diagram showing the structure of the filter element support member 22 in FIG. 2. 6a-6b are a series of diagrams showing the structure of the plug 34 in FIG. Fig. 7 is a partial cross-sectional view showing a centrifugal connection type solution transfer and processing system for a through solution, which is constructed according to the second embodiment of the present invention. 8 is a partial cross-sectional view showing a tube in the second embodiment of the withdrawal solution transfer / processing device in FIG. 7 and having a screw cap 122. FIG. Fig. 9 is an exploded cross-sectional view showing the second embodiment of the micro solution transfer / processing device in Fig. 7, and omitting the upper part or source tube 110b. Fig. 10 is a top view showing the embolism 150 of the second embodiment of the withdrawal solution processing device in Fig. 7, the pseudo edge of the plug (please read the back-end precautions # 結 写 this page) binding · binding-line · this paper Standards are used in China National Standards (CNS) Grade 4 (210X297 male dragon) _ 4 81. 4. 10,000 sheets (H) 209853 Λ 6 Π 6 Printed by the Ministry of Economic Affairs, Central Standards Bureau, Beigong Consumer Cooperative Cooperative 5i. DESCRIPTION OF THE INVENTION (3) It was obtained by the line 10-10 of FIG. 9 and the direction of the arrow. 11 is an isometric view of the plug 150 of the device of the second embodiment of FIG. 11a is a perspective view showing the tool 162, which is used to insert the plug 150 into the inner cylinder 130c of the connector 126. FIG. 12 is a top view of the connector 126 of the micro solution processing apparatus of the second embodiment, showing the The membrane support part. Fig. 13 is an exploded cross-sectional view of the membrane support portion of the connector of the withdrawal solution processing apparatus of the second embodiment, which is taken along the line 13-13 of Fig. 12 and the direction of the arrow. Fig. 14 is a side view of the joint 170, which is used to fix the second embodiment of the withdrawal solution processing apparatus of the present invention in a centrifugal rotor. 15 is a side view of the cross section of the joint 170 of FIG. 14. Drawing 16 is an isometric view showing how the second embodiment of the micro solution processing device is incorporated in the joint (shown in cross section). Fig. 17 is a partial cross-sectional view of the function diagram showing that the second embodiment of the solution processing apparatus of the present invention is fixed by a joint and is located in a fixed-angle rotor. Detailed description of the present invention The following detailed description pretends to illustrate the present invention by way of example and does not limit the original invention. This detailed description enables those skilled in the art to make and use the invention clearly, and explains several embodiments and different aspects of the invention and the uses of the invention, including the applicant currently considers to be the best of the invention Appearance. FIG. 1 is a pseudo schematic diagram illustrating the overall principles and method steps of the connection type complete solution transfer and treatment system and method of the present invention. The presently preferred embodiment of the present invention is about a processing method and system, using ultrafiltration membrane in high-performance liquid chromatography, so as to pre-treat the solution. Referring to FIG. 1 (a), the researcher first performs a predetermined chemical reaction, such as an enzyme reaction, in a container or test tube A. The resulting solution or product is represented by the dotted line in FIG. 1. A cap (not shown) can be used for the open end of the test tube. • Next, as shown in FIG. 1 (b), at the end of the reaction, the experimenter removes the cap (not shown) from the test tube A and engages the end of the connector C to the opening of the test tube A. The second container is represented by the test tube B in the figure, which has the same shape as the test tube A, and is joined to the other end of the connector C in an inverted manner. Connector C includes one of the ultrafiltration membranes (not shown (please pay attention to Mift's back then fill in this page). Binding-Order-Line &<; This paper standard is easy to use Chinese trapped home standard (CNS) A 4 Specifications (210X297; 81. 4. 10,000 sheets (H) 209853 Λ 6η 6 Ministry of Economic Affairs Central Standards Bureau Zhen Gongxiao ft. Cooperative Duprinting V. Description of Invention (4)). Second, as shown in Figure 1 (c) As shown, the treatment bar unite forms tubes A and B, and connector C is inverted '(as indicated by the arrow of the cross), and is inserted into centrifugal separator D, and then the centrifugal separator is rotated. In this example In tube A is called "source tube" or "reaction tube", and tube B is called "target tube". Because of the centrifugal effect (see Figure 1 (d)), the sample in reaction (source) tube A The solution passes through the ultrafiltration membrane in connector C and enters the target tube B. If the solvent molecules are removed and there is still a predetermined or larger molecular weight, the ultrafiltration membrane is trapped. As mentioned above, according to this In several embodiments of the invention, the centrifugation is started when the anti-cold tube containing the sample solution and the tube for centrifugation are integrated with the connector with the ultrafiltration membrane Since there is no need to use a bow dropper tube to transfer the solution between the source tube and the target tube, there will be no loss of solution due to the residual solution in the microdropper device. At the same time, the dropper tube is also avoided Possibly contaminated. Furthermore, the solution is transferred into the phase tb by " direct perfusion method (this method pseudo-pours the contents of the reaction (source) tube into the target tube). Due to the completeness of the centrifugal filtration, Therefore, the present invention will not cause the residual of the sample solution in the wall of the internal source tube. When performing a reaction in many steps, the processing in the above Figure 1 (a)-(d) can be repeated in each step after the second step , That is, use the tube B (the original target tube and now has the filtered solution, see Figure 1 (d)) as the new reaction (source) tube A and add the new target tube B 'and so on. Figure 2 It is a partial cross-sectional view of a complete solution transfer / processing system constructed according to the first embodiment of the present invention. The complete solution processing system 1 is broken into a connected state, which is consistent with the schematic diagrams of FIGS. 1 (c) and (d) The withdrawal solution processing system 1 includes a reaction or source tube 10a The target tube 10b, each having an open end 12a, 12b facing each other, is joined by a connector assembly 16. The tubes 10a, 10b have the same shape and are preferably made of plastic materials often used in centrifugal applications. For example, made of polypropylene or polyethylene, the tubes 10a and 10b are respectively consistent with the tubes A and B of FIG. 1, and the connector assembly 16 is consistent with the connector C of FIG. 1. The connector assembly 16 includes Connector member 17, membrane holder 22, and a plug 34. The connector member or connector 17 has two different connector ends for engaging the tube openings 12a, 12b of the tubes 10a, 10b The two connector ends include a first connector end 18, which is a pseudo-open type member and has a tapered bearing inner wall 19 to form an appropriate edge wall 14a, 14b corresponding to the outer edge wall 14a, 12b of the corresponding tube opening 12a or 12b Lianhe; In addition, there is a second connector end 20, which has a male spiral portion 19 along the outer edge wall of the chopstick to correspond to the female spiral portions 15a, 15b on the inner edge wall of the corresponding tube openings 12a, 12b Join. In Figure 2, the connector 17 has the first connector end (please read the precautions before filling in this page) Binding · Order-Line. This paper "standard" uses the Chinese Η home «quasi (CNS) T4 specifications ( 210X297 male; ";) 6 81. 4. 10,000 sheets (Η) Λ 6 Π6 09853 5. Description of the invention (5) 18 fits with the outer edge wall Ua of the tube opening i2a of the source tube i〇a, and the second connection The female spiral portion 19 of the device end 20 is threadedly engaged with the inner female spiral portion 15b of the tube opening 12b of the sassafras tube 10b. (Please read the precautions before writing this page) Membrane holder 22 has a male spiral portion 24 formed along an outer edge wall, and the size of its screw thread can accept the female spiral portion of the inner edge wall of the tube openings 12a, 12b 15a. 15b thread. In this example, the outer peripheral wall male spiral portion 24 of the membrane holder 22 is engaged with the inner peripheral wall female spiral portion 15a of the source tube opening 12a. The membrane support 22 is adjusted to receive an ultrafiltration membrane along the bottom support surface (see Figure 5). A plug 34 is used to ensure that the membrane is fixed in the membrane holder 22. FIG. 3 is an enlarged view of two values, showing the detailed structure of the tube 10. Here, the tube 10 may be the source tube 10a or the target tube 10b. Part (a) in Fig. 3 is a plan view of the opening 12 of the tube 10, and part 0 >) is a sectional view showing the flat outer edge wall 14 of the tube opening 12 and the inner edge wall 15 of the female spiral portion. The wall thickness "t" of the tube opening 12 is preferably slightly tapered toward its free end so that the receiving connector end 18 of the connector member 17 can be easily inserted. Fig. 4 is two enlarged views showing the structure of the connector 17 of Fig. 2, in which part (a) is a plan view and part (b) is a cross-sectional view. The cross section of the connector 17 is substantially circular and includes an inner stop surface or ledge 19, so that when the modular device 1 is fully engaged (see FIG. 2), the tube opening 12 and the end of the membrane holder 24 abut against 19 It is limited to a contiguous state. The connector 17 has a central hole 23 so that the solution can be transferred from the first tube to the connected second tube. Fig. 5 is an enlarged series of four views showing the structure of the membrane support member 22 of Fig. 2, wherein (a) part is a top view (supporting surface 26 is omitted); (b) part is a cross-sectional view; (c) Part is a side view: and part (d) is an enlarged bottom view showing the structure of most perforations or ducts 28 formed in the bottom wall 26 (see part a). However, for clarity, the catheter 28 is not shown in the cross-sectional view of part (b). Ministry of Economic Affairs Central Standards Bureau κζ 工 消 $ :; Cooperative print! 52. Figure 6 is a series of two bows, showing the structure of the circular plug 34 of Figure 2, part (a) is a side view, and * ( Part b) is a top view. The plug 34 is like a ring or a circular member, and has a ring rib 36 on its outer edge wall 38, which is like a corresponding groove 27 for inserting into the inner green wall 29 of the membrane holder 22 (see FIG. 5). The combination of the two tube 10a and 10b as described above can achieve efficient transfer solution and centrifugal treatment at the same time (see Figure 1). Fig. 7-13 shows the second embodiment of the device for transferring the withdrawal solution of the present invention to the system, which is based on the member 81. 4. 10, 〇〇〇 'in this picture (H) The paper size is easy to use the Chinese country. Standard (CNS) IM specification (210X297 male dragon) _ 7 209853 Λ 6 Π 6 Standard of the Ministry of Economic Affairs Λ, printed by the Employee Explosive Cooperative V. Invention description (6) 100 to represent. Referring to FIG. 7, a second embodiment 100 of a micro-solution processing system includes two slap-like containers or tubes 110a, 110b each having an open end 112a, 112b, which are pseudo-connected by a connector assembly 126 when in use所 接. The connector assembly 126 of the second embodiment includes two main components, namely a connector / receiver fastener 127 and a plug 150. As shown in FIGS. 7 and 9, the connector member 127 has a double-ring groove structure, which has a cylindrical shell portion or sleeve 128 of an outer thread surrounding an inner cylindrical portion 130 and is integrally joined by a radially extending transverse web 132. The outer shell (sleeve) 128 and the inner cylinder form two connector ends, including a first threaded connector end 136 and a second sliding connector end 140. The housing portion or sleeve 128 is preferably saw-toothed or knurled at 137 to facilitate operation by the ribbed user. Similar co-rib holding surfaces 120a, 120b may be provided on the outer surfaces of the tubes 110a, 110b. In this example, the threaded connector end 136 includes female threads 114a provided along the inner edge wall of the outer cylindrical portion 128 to engage the outer edge wall of the tube opening 112a of the tube 110a. Furthermore, the sliding connector end 140 is attached to the open end 112b and the male thread 114b of the target tube 110b. The cylindrical portion 130 of the connector 127 also includes a lateral membrane support surface or area 134. In use, the connector member 127 is engaged in the tube opening so that the inner cylinder 130 of the membrane holder is pseudo-engaged in the tube opening 112b of the target tube 110b. The membrane holder surface 134 of the inner cylinder 130 forms an orifice plate, and the ultrafiltration membrane 156 is located on the plate. The ultrafiltration membrane 156 is firmly positioned by a plug 150, which is engaged in the inner cylinder 130 when in use. The preferred height of the wall of the tubular plug 150 and the inner cylinder 130 is to ensure that all the solution pots are retained in the cylindrical volume formed by the holes of the tubular plug 150 during centrifugation, so that the bending represents the loss of solution The lunar surface will not exceed the plug 150 or the cylinder 130. This capacity is generally between 500 liters and 600 liters in total solution work. In addition, the wall height of the plug 150 is preferably slightly lower than the height of the surrounding wall portion of the inner cylinder 130, so that the tapered end 158 of the plug 150 forms a gradual transition to promote the liquid from the source tube to the centrifugal action All of the following flows into the target tube. In addition, the end wall forming the opening of the inner cylinder 130 is preferably removed at a point of 166 (see FIG. 9) to further promote the entire flow of liquid into the inner cylinder 130. FIG. 8 shows a single tube 110 in a temporal view, which has a screw cap 122 for screwing on the external male thread 114 of the tube opening 112. FIG. The cover 122 includes an O-ring 124 to ensure that liquid is not lost. The thread on the cap 122 is useful for sealing the source tube 110a (e.g., after an enzyme reaction has occurred), or for sealing a labeled tube after the desired treatment has been obtained with a trace amount of solution. Referring to FIGS. 9-11, the plug 150 includes a plurality of notch portions disposed at equal intervals along the top edge wall 154. (Please read the precautions before filling in this page) Binding & Binding-Line-f Paper size for easy use Β ΒΓ # · 楳 quasi (CNS) Ή regulations (210x297 public; Jt) _ 8 81. 4. 10,000ft (H) 209853 Λ 6η 6 Printed by the Ministry of Economic Affairs of the Ministry of Economic Affairs and Labor only to be printed by the cooperative. V. Description of the invention (7) Some notches 160 are provided to assist the insertion of the plug into the connector assembly 126 In the cylindrical membrane holder 130. The embolism 150 preferably includes a bow-shaped slot (not shown) formed along the outer pelvis wall to assist air exchange and thereby expel any sinking when the embolization 150 is engaged in the inner cylindrical membrane support 130 Air in the inner cylindrical membrane holder 130 and the plug 150. FIG. 11a shows an exemplary tool 162 for inserting the plug 150 into the inner barrel 130. FIG. The tool 162 preferably includes an axially extending rim projection member 164 to engage the recessed portion 160 of the tubular plug 150. The top edge 154 of the plug 150 is preferably tapered at 158 to ensure that all withdrawal solutions are When in use, it flows to the ultrafiltration membrane and will not be trapped above the embolization edge 154. Similarly, the shape of all the edges of the notch 160 is preferably round to promote and ensure fluid hip flow. 12 and 13 show details of the orifice-like membrane holder region 134 of the inner cylinder 130 of the connector 127. The perforated plate area 134 includes a plurality of arcuate and semi-arcular perforations or ducts 142 separated by rib portions 144. The outer edge of the membrane support region of the orifice plate 134 includes a slightly raised rib member 146, which has a peak at the time when the plug 150 is inserted into the inner cylinder 130, it is aligned with the lower end wall 152 of the tubular plug 150 . It can be seen by referring to FIG. 13 (the embolism 150 and the membrane 152 are pseudo-represented by ghost images). In this way, the membrane 156 can be kept tight and prevented from being removed due to the engagement of the bottom end wall 152 with the raised rib member 146. _ 14-16 shows that the joint 170 can be used to couple the withdrawal solution transfer / treatment system 100 of the first or second embodiment to the receiving sleeve of the centrifugal rotor. Because more connection members of the micro solution processing system can increase the circumference of the surrounding, the thorough solution processing system has a slightly longer outer radius than the traditional centrifuge tube. Therefore, it is better to have a sleeve with a wider diameter in the centrifugal rotor to receive the double tube / connector system. For this purpose, a joint 170 is used to ensure that the entire solution strip 100 is firmly and firmly engaged in the centrifuge rotor. The cross section of the joint 170 is cylindrical, and the size of its inner diameter can ensure a close tolerance joint when the connection-type withdrawal solution strip is inserted therein. The outer surface of the joint 170 has a laterally extending surrounding wall member 174 (-annular flange). When it is inserted and engaged in the receiving sleeve 176 of the centrifugal rotor, it serves as a stop member and a support frame. Figure 17 shows that the modular device 100 is located in the joint 170 and is inserted into a suitable socket or hole 176 of the rotor Γ78. The connector includes an opening 180 with a smaller radius at its bottom end, and its size can be engaged with the outside of one of the tubes of the withdrawal solution system 100 at a position adjacent to the connector assembly along the bottom tube, thereby stripping The bottom end 182 of the control device 100. does not contact the bottom 184 or the side wall 185 of the centrifugal rotor 178. The length of the joint 170 is higher than the upright wall 186 of the wall member 174 is sufficient to ensure the connection of the complete solution processing device during centrifugation (please read the notes beforehand Sun fills this page) -Line · This paper uses the Chinese National Sample (CNS) T4 specification (210x297 g *) _ 9 81. 4. 10,000 sheets (H) Λ 6 It 6 209853 5. The description of the invention (8) has appropriate support. Figure 16 shows that the front of the joint can be cut off at 188 (indicated by dashed lines), leaving only the high-back support portion higher than the upper joint wall of the flange or wall member 174. (The cut-out portion is represented by the member 171) In this way, a light-weight joint is formed while still having sufficient support to reduce the pressure exerted on the conventional device by the centrifugal force. Although the above-mentioned embodiment is preliminarily concerned with the pre-processing system of high-performance liquid chromatography, in which an ultrafiltration membrane is commonly located in the connector part, in another embodiment of the present invention, a pre-processing system of affinity for lotus It is performed by providing an affinity functional film in the connection. For example, the connector membrane may contain antibodies or antigens and lectins or an ion exchange membrane, or a membrane with other suitable functions. Although the present invention has been explained and explained in detail, we should understand that those of ordinary skill in the industry should be able to make various modifications within the scope of the invention without departing from the spirit of the invention. Therefore, the meaning of the present invention is defined by the following patent application scope with reference to the foregoing description. (Please read the precautions first and then write this page) Staff of the Ministry of Economic Affairs Bureau of Standards of the Ministry of Economic Affairs printed as a cooperative ^ 81. 10,000 sheets (H) This paper standard uses the BB's "CNS" T4 specifications ( 210x297 public «:> _