CN213506927U - Cell separation filters collection device - Google Patents

Abstract

The utility model discloses a cell separation filters collection device belongs to biological experiment apparatus technical field. The cell separating, filtering and collecting device comprises a filter screen, an inner ring, a pressing ring and a liquid receiving hopper. The filter screen is clamped and fixed between the inner ring and the pressing ring which are formed by sleeving. The liquid receiving hopper is provided with a cylindrical hopper body, the lower part of the hopper body is integrally formed with a conical surface which converges downwards, and the bottom of the conical surface is provided with a liquid outlet. The combination body formed by the inner ring, the pressing ring and the filter screen is fixed on the upper part of the bucket body, and a space for accommodating a sample to be filtered is formed. The device has improved the overall structure of traditional stainless steel cell sieve, makes its convenience more and save the experiment cost in experimental operation process. Simultaneously, reduce the experiment consumptive material and use for the disposable cell sieve of plastics, the waste product produces.

Description

Cell separation filters collection device

Technical Field

The utility model relates to a cell separation filters collection device belongs to biological experiment apparatus technical field.

Background

In the process of cell biology research, whether physiological functions such as cell physiological characteristics, cell invasion, apoptosis and phagocytosis are carried out, or the influence of environmental factors on cell marker expression and the application of drug screening and the like are carried out, the premise is to obtain ideal uniform single cell suspension, so that the subsequent experiment can be ensured to be smoothly carried out, and a more accurate result is obtained.

The cell sieve is a separation, filtration and collection device commonly used in cell culture, impurity filtration, cell dispersion, sample separation and other links. The cell sieves on the Chinese market are many in variety, but the BD FALCON cell sieves in the United states, the Korean SPL cell sieves and the Toscience stainless steel cell sieves are most widely adapted. The cell sieve can be roughly divided into two types, one type is a disposable cell sieve made of materials such as a plastic nylon filter screen and the like, and the cell sieve is suitable for a single cell separation experiment using a 50mL centrifuge tube and cannot be recycled. The other is a filter-spoon type cell sieve made of stainless steel. The stainless steel screen mesh is a common experimental article in a laboratory, and can be cleaned, sterilized and reused. The use of plastic disposable cell screens increases waste generated during biological experiments. And the stainless steel screen is used, so that the generation of experimental wastes is reduced, and the pressure of the ecological environment can be reduced.

However, due to the material problem, the size of the stainless steel screen is generally larger than that of the centrifuge tube used in all the conventional biological experiments, and in the single cell separation experiment process, the stainless steel screen is used for filtering cell sediments, and the cell-containing liquid is transferred into the centrifuge tube with a proper volume. So as to achieve the purpose of separating single cells from tissue residues. However, in the process of filtration, the stainless steel screen has a large surface area, so that liquid flows down from any part of the surface of the stainless steel screen, even flows to the outside of a centrifugal tube connected below the stainless steel screen, cells are lost, the accuracy of an experiment is affected, and even the experiment fails.

SUMMERY OF THE UTILITY MODEL

Receive the technical problem that the device mismatching leads to the experimental sample to run off for producing that it is more, separator and centrifuging tube etc. to accept that the production discarded object that exists in the solution present biological cell separation filters the collection process, the utility model provides a cell separation filters collection device, the technical scheme who takes as follows:

a cell separating, filtering and collecting device comprises a filter screen 4, an inner ring 4, a pressing ring 2 and a liquid receiving hopper 1; the filter screen 4 is clamped and fixed between the sleeved inner ring 4 and the pressing ring 2; the liquid receiving hopper 1 is provided with a cylindrical hopper body 11, a conical surface 16 which is converged downwards is integrally formed at the lower part of the hopper body 11, and a liquid outlet is formed at the bottom of the conical surface 16; the combination of the inner ring 4, the pressing ring 2 and the filter screen 4 is fixed on the upper part of the bucket body 11, and a space for accommodating a sample to be filtered is formed.

Preferably, the material of the inner ring 4 is stainless steel.

Preferably, the liquid receiving hopper 1 is of an integrally formed structure; the bottom of the hollow cylindrical bucket body 11 is provided with an inverted conical surface 16, the bottom of the conical surface 16 is provided with a liquid outlet, and a liquid outlet pipe 13 with a downward opening is arranged at the liquid outlet; a semicircular arc-shaped side edge I12 is arranged at the bottom of the bucket body 11; the bottom of the arc-shaped side I12 is provided with a half ring groove 15.

More preferably, the pressure holding ring 2 is an integrally formed structure; comprises an arc side II22 which can be spliced with the arc side I12 to form a complete ring shape, and an arc narrow side 23 connected with the two free ends of the arc side II 22; the arc-shaped narrow edge 23 can be inserted and fixed in the half-ring groove 15.

More preferably, the arc-shaped side II22 is provided with a handle 21; the lower part of the handle 21 is provided with a clamping groove 24.

More preferably, a fixing buckle 14 which can be matched and connected with the clamping groove 24 is arranged at the upper part of the liquid receiving bucket 1 which is not provided with the arc-shaped side I12; a buckle 141 which can be clamped in the clamping groove 24 is arranged at the upper part of the fixing buckle 14; the fixing buckle 14 is made of hard and flexible material, and can be bent downwards to make the buckle 141 separate from the slot 24.

Preferably, the outer diameter of the inner ring 4 is equal to the inner diameter of the pressure ring 2.

More preferably, the outer edge of the upper surface of the inner ring 4 and the inner edge of the lower surface of the pressure ring 2 are rounded to prevent the two from being cut off due to shearing force when the two are combined.

Compared with the prior art, the utility model discloses the beneficial effect who obtains is:

the device provided by the utility model improves the overall structure of traditional stainless steel cell sieve, makes its convenience more and sparingly experiment cost in experimental operation process. Simultaneously for disposable cell sieve of plastics reduces experiment consumptive material and uses, waste product production. The device of the utility model adopts a combined structure, increases the convenience and the practicality of using the cell sieve, and reduces the use cost of the cell sieve. Meanwhile, the liquid receiving device is additionally arranged at the bottom of the filter screen, so that the device can be applied to experimental articles with different opening sizes to carry out single cell separation experiments, and the experimental failure caused by the fact that liquid drops fall and are not received by a centrifugal tube in the experimental process is reduced.

Drawings

Fig. 1 is a schematic perspective view of a separation, filtration and collection device according to a preferred embodiment of the present invention.

Fig. 2 is a schematic diagram of the exploded structure of fig. 1.

Fig. 3 is a schematic front view of a liquid receiving bucket of a separation, filtration and collection device according to a preferred embodiment of the present invention.

Fig. 4 is a schematic perspective view of a pressing ring of a separation, filtration and collection device according to a preferred embodiment of the present invention.

Wherein, 1, the liquid receiving hopper is connected; 2, pressing and holding the ring; 3, filtering by using a filter screen; 4, an inner ring; 11, a bucket body; 12, arc-shaped side edges I; 13, a liquid outlet pipe; 14, fixing buckles; 15, half ring groove; 16, a conical surface; 21, a handle; 22, arc side II; 23, arc-shaped narrow edges; 24, a card slot; 141, buckling.

Detailed Description

In the following description of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "top", "bottom", "inner", "outer" and "upright" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the following description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; the connection may be direct or indirect via an intermediate medium, or the connection may be internal to both components. To those of ordinary skill in the art, the specific meaning of the written terms in the present invention can be understood as a specific case.

In addition, in the following description of the present invention, the meaning of "a plurality", and "a plurality" is two or more unless otherwise specified.

The materials, reagents, devices, apparatuses, methods and processes used in the following examples are not specifically described, and are all materials, reagents, devices, apparatuses, methods and processes which are common in the art, and are commercially available to those skilled in the art or can be routinely set up according to specific needs without any creative effort.

The present invention will be described in further detail with reference to the accompanying drawings, but the present invention is not limited by the following detailed description.

Fig. 1 is a schematic perspective view of a separation, filtration and collection device according to a preferred embodiment of the present invention. As can be seen from FIG. 1, in the preferred embodiment, the device is provided with a receiving bucket 1 which is hollow cylindrical as a main body, a pressing and holding ring 2 is fixedly inserted into the upper part of the receiving bucket 1, and a nylon filter screen 3 is arranged at the joint of the upper part of the receiving bucket 1 and the pressing and holding ring 2. The main body of the liquid receiving bucket 1 is a bucket body 11, an inverted conical surface 16 (not shown) is arranged below the bucket body, and a liquid outlet pipe 13 is arranged at the bottom of the conical surface 16. The upper part of the bucket body 11 is provided with a semicircular arc-shaped side I12, the arc-shaped side I12 is tightly combined with the arc-shaped side II22 of the pressing ring 2 to form an annular space, and the bottom of the space is provided with a filter screen 3 mainly used for accommodating samples to be filtered. Meanwhile, in order to facilitate the extraction and insertion of the pressing ring 2, a handle 21 is arranged on the outer side surface of the arc-shaped side I22.

Fig. 2 is a schematic diagram of the exploded structure of fig. 1. As shown in fig. 2, an inner ring 4 made of stainless steel is further provided inside the pressure ring 2. The inner diameter of the pressure ring 2 is equal to the outer diameter of the inner ring 4, and the filter screen 3 is positioned in the middle when the two are sleeved, so that the three are firmly combined into a whole. After the combination is formed, the arc narrow edge 23 connected with the free end surface of the arc side II22 of the pressing ring 2 can be inserted into the half ring groove 15 at the bottom of the arc side I12, so that the whole device is formed into a whole.

Fig. 3 is a schematic front view of a liquid receiving bucket of a separation, filtration and collection device according to a preferred embodiment of the present invention. As can be seen from FIG. 3, in the preferred embodiment, the liquid receiving bucket is of an integrally formed structure, the middle part is of a hollow cylindrical structure, the bottom part is connected with the liquid outlet pipe 13 at the bottom part through an inverted conical surface 16, the upper part is an arc-shaped side I12, and the upper part of the bucket body 11 opposite to the arc-shaped side I12 is fixedly connected with a fixing buckle 14. The upper portion of the fixing buckle 14 is provided with a buckle 141, and the fixing buckle 14 is made of hard flexible material and can be elastically deformed downwards.

Fig. 4 is a schematic perspective view of a pressing ring of a separation, filtration and collection device according to a preferred embodiment of the present invention. As can be seen from FIG. 4, in the preferred embodiment, the main body of the pressure ring 2 is formed by splicing semicircular arc-shaped sides II22 and arc-shaped narrow sides 23 which have different widths and the same inner diameter to form a circle. A handle 21 is arranged on the outer side of the arc-shaped side II22, a clamping groove 24 is arranged at the bottom of the handle 21, and the clamping groove 24 can be matched and connected with a buckle 141 at the upper part of the fixing buckle 14, so that the arc-shaped narrow edge 23 is tightly fixed in the semi-ring groove 15, and the device is formed into a whole.

The using process and principle of the device are as follows: when the filter is used, the circular large-diameter filter screen is firstly paved on the inner ring, and the pressing ring 2 is sleeved outside the filter screen, so that a combination of the three is formed. Then, peg graft the combination body in connecing the semi-ring groove of liquid fill, the buckle card is gone into to the draw-in groove when inserting the end, fixes device whole then can begin the experiment. When the pressing ring and other assemblies need to be removed after the test is finished, the clamping buckle is only needed to be pressed downwards to enable the clamping buckle to be separated from the clamping groove, and the assemblies can be pulled out. The filter screen can adopt a disposable nylon filter screen.

Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made by those skilled in the art without departing from the spirit and scope of the invention.

Claims (8)

1. A cell separating, filtering and collecting device comprises a filter screen (3), and is characterized by also comprising an inner ring (4), a pressure holding ring (2) and a liquid receiving hopper (1); the filter screen (3) is clamped and fixed between the sleeved inner ring (4) and the pressing ring (2); the liquid receiving hopper (1) is provided with a cylindrical hopper body (11), a conical surface (16) which converges downwards is integrally formed at the lower part of the hopper body (11), and a liquid outlet is formed at the bottom of the conical surface (16); the combination body formed by the inner ring (4), the pressing ring (2) and the filter screen (3) is fixed on the upper part of the bucket body (11) and forms a space for accommodating a sample to be filtered.

2. The cell separation, filtration and collection device according to claim 1, wherein the inner ring (4) is made of stainless steel.

3. The cell separation, filtration and collection device according to claim 1, wherein the liquid receiving hopper (1) is an integrally formed structure; the bottom of the hollow cylindrical bucket body (11) is an inverted conical surface (16), the bottom of the conical surface (16) is a liquid outlet, and a liquid outlet pipe (13) with a downward opening is arranged at the liquid outlet; a semicircular arc-shaped side I (12) is arranged at the bottom of the bucket body (11); the bottom of the arc-shaped side I (12) is provided with a semi-ring groove (15).

4. The cell separating, filtering and collecting device according to claim 3, wherein the pressure holding ring (2) is an integrally formed structure; comprises an arc side II (22) which can be spliced with the arc side I (12) to form a complete ring shape, and an arc narrow side (23) connected with two free ends of the arc side II (22); the arc-shaped narrow edge (23) can be fixedly inserted into the half ring groove (15).

5. The cell separating, filtering and collecting device according to claim 4, wherein a handle (21) is provided on the arc-shaped side II (22); the lower part of the handle (21) is provided with a clamping groove (24).

6. The cell separating, filtering and collecting device according to claim 5, wherein a fixing buckle (14) which can be connected with the clamping groove (24) in a matching way is arranged at the upper part of the liquid receiving hopper (1) which is not provided with the arc-shaped side I (12); a buckle (141) which can be clamped in the clamping groove (24) is arranged at the upper part of the fixing buckle (14); the fixing buckle (14) is made of hard and flexible materials and can be bent downwards to deform so that the buckle (141) is separated from the clamping groove (24).

7. The cell separation, filtration and collection device according to claim 1, wherein the outer diameter of the inner ring (4) is equal to the inner diameter of the pressure ring (2).

8. The cell separating, filtering and collecting device according to claim 7, wherein the outer edge of the upper surface of the inner ring (4) and the inner edge of the lower surface of the pressure holding ring (2) are rounded.

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