TW203556B - Method of making hair nourishing solution - Google Patents

Abstract

Method of making hair nourishing solution containing the characteristic of: making a solution of dregs containing rice bran, bean, red bean and bean curd; adding chymotrypsin, trypsin or pepsin in the solution at the ratio of 1:1000, or adding Aspergillus at the ratio of 1:100; adding buffer solution at the pH of 6-11; maintaining the solution at 35-45xC for 5-10 hours so that the solution will be fermented to obtain the main compositions of a hair nourishing solution.

Description

A6 B6 203556 V. Description of the invention (f) [Industrial application field] The present invention is like a method of splitting a hair nourishing liquid, especially a compound of lipase and skin sugar, or _ e. It is a method of producing hair growth liquid mainly composed of fermentation products by acting on a base containing plant proteins and plant lipids such as rice bran. [Problems to be solved by conventional techniques and inventions] So far, the mechanism of hair removal and hair growth of human plaques is unknown. Generally speaking, it is believed that the main causes of hair removal and hair growth are false blood circulation, poor metabolism and excessive metabolism. The presence of male hormones, scalp inflammation, allergies, etc. According to this, hair can be divided into a part that grows on the surface of the scalp, and a part of the hair root in the scalp. The part of the hair bulb at the base of the hair root has a tissue called hair mother cell. In addition, around the hair bulb, there are many evacuated blood vessels around the garden, and the blood from the blood tube is taken out, and the hair mother cells can take up the nutrient and repeat the division, and the hair is born. However, if the blood cannot be supplied to the cells sufficiently, the cell division in the hair mother cells will not be able to proceed actively, which will hinder the growth of the hair. Therefore, in order to promote hair growth and hair growth, there is a saying that the necessary condition is to promote the blood circulation of the scalp, so that the eaves of the hair mother cells can be activated. Also, there is a saying that the hair follicles surrounding the hair have an enzyme called 5α-progenase, which is combined with male hormonal, and transformed into 5α_ 二 氲 睪丸 脂 甲, 1 5c (—The Dihydrobi Pill Fatty Ketone Conference Paper is again applicable to the China National Standard (CNS) A4 specification (210 X 297 mm) &lt; jfr first read the notes on the back and then write this page)丨 Installed and ordered. Printed by the Ministry of Economic Affairs Central Standards / CK Industrial and Consumer Cooperatives 81.9.20,000 203556 V. Description of the invention (1) (Please read the precautions on the back before writing this page) Transfer to the hair nipple through the blood vessels and inhibit The activity of amyl-based circulating enzymes in hair mother cells causes the hair mother to divide and protract, which causes the hair pack to shrink gradually, resulting in thinning hair and baldness. Although there are many kinds of sayings about hair removal and hair raising institutions as mentioned above, there is no definitive theory. So far, many hair nourishing agents proposed by the vegetable industry, etc., according to various doctrines, contain substances that can promote blood circulation of the scalp as effective ingredients, or substances that activate the function of hair mother cells. There is no hair-retaining fluid with sufficient hair removal prevention effect, and good hair growth and hair growth effects. In view of these facts, the inventors have made a keen study in order to develop a hair-growing liquid with a good hair-growing effect, and finally found that a mixture of lipase and prion, or koji, contains, for example, vegetable protein and The fermented product obtained by the matrix action of plant-based lipids such as rice seeding has sufficient hair removal prevention effect, and has excellent hair-growing and hair-growing effects. After repeated research, the invention was finally completed. [Means for Solving the Problems and Their Functions] Hereinafter, the method for preparing the hair growth liquid of the present invention will be described. Printed by the R Industry and Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs according to the method of the present invention, which is to be taken: the extraction of vegetable protein and vegetable lipids containing rice bran, soybean pressed soybean meal, red gram pressed soybean meal, bean curd residue Aqueous base, to which the chymotrypsin, papain, evening protein, etc. are added at a ratio of 1: 1000 to decompose the above vegetable proteins and vegetable fats, or at a ratio of 1: 100 After the bacteria, add the retarding liquid to make the pH of the above-mentioned aqueous base to be 6 ~ 1 1, 81.9.20,000. The paper and paper are again applicable to the Zhongyuan® home standard (CNS> A 4 specifications (210 X 297 mm) 203556 A6 B6 5. Description of the invention (3) (please read the note A on the back and fill in this page), and then place the above-mentioned aqueous substrate at 5 to 4 513 ffl degrees for 5 to 10 hours to ferment it, and prepare the yarrow hair liquid The main ingredient in the meal hair liquid of the present invention, as the aqueous base, may be an aqueous base obtained by adding water to the following base; for example, the base may be such as rice bran, wine meal, wheat sprouts, bran, cyanine Shells such as Mai Xuanyi and Barnyard Barnyard Shell, Such as soybeans, bean curd dregs, soy milk, red beans, soybean powder, m-flavored meal and other beans, such as potatoes, natural taro, green potatoes, etc., in addition to black tea meal, honey, sesame, peanuts, Lotus seeds, Mingyue leaves, purple leaves, wakame leaves, kelp, etc. Especially good are rice bran, wine meal, bran, kaffir, wheat sprouts, sesame, tofu dregs, soybeans, red beans, etc. Vegetable fats are included in the base in an average manner. Enzymes that break down the role of plant-based proteins in bases include lipids and prions. In addition, powdery enzymes can be added to them. Enzymes. In addition to fat sugars and prions, you can use koji with the function of producing a variety of enzymes in the raw meat. The WK Industrial and Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economy printed the lipase for use in acid The enzymes obtained from various emblems, yeast barley, fine curium, flill liquid, and opium poppy, represented by sugar liquid waist orchid, are used in Fan Xi. In terms of strontium, pancreas fat orchid and hepatophyllum can be used , Tuber koji fat sugar, FIB lipase, castor oil Enzymes, etc. In addition, in the case of prion enzymes, the best is the acidic skin sugar in terms of the pH of the bighead. Among them, it is better not to contain specific amino acids except for the amino acid residues of the element itself. The active group, at the same time, in order to play the role of enzymes, no specific reagents such as protease, trypsin, chymotrypsin, etc., or 81.9.20,000 of cyanide can be used. This paper is also applicable to the standard of the Chinese family (CNS &gt; A4 specifications ( 210 X 297 mm) 203556 A6 B6 _ V. Description of the invention (from) ft acid m, ascorbic acid, cystine acid, glutamine sulfide and other raw materials _ activated prion; papaya protein stuffing, fig It can be used for phytonic enzymes such as prase, enzymes widely distributed in animal cells, or most of autolytic enzymes. More specifically, its examples include: chymotrypsin, trypsin filling, pu protease, carboxyl enzyme, cell autolytic enzyme a, cell autolytic enzyme B, cell autolytic enzyme C ', cell autolysis Enzyme B, Cell Autolysis IV, Cholesterol Aldehyde I,》 Dioxoamide I, Leucine Amino Groups are preferred, Amino Groups are three preferred groups, Amino Ethyl Glycine Soluble Fruit Mash, Preserved Enzymes, proline enzymes, cytosolics, thrombin, papaya protein tincture, fig prion, prion brewing of staphylococcus, C1, histolyticuB limb enzymes and glandular brewing, etc. 1 份。 Enzymes that can decompose vegetable protein and vegetable lipids, with respect to 100 parts by weight of the above-mentioned aqueous base, add 0.1 part. In addition, in the case of echinacea, its decomposing power is weaker than that of the lipase and prion enzyme, so it is added at a ratio of 1 replacement part relative to 100 parts by weight of the substrate. The above-mentioned aqueous base is added with a Niu Heng liquid and adjusted to have a pH value of 6 to 11. There are two slow-balanced liquid acid-colic acid strips, potassium phosphite-potassium phosphate strips, oxyacid-florona strips, hydroxyacid-triaminomethane strips, hexanoic acid-borax strips, borosilicate monohydrate strips, m-acid monoamine methylmalonate bar, ammonium nitride-ambient bar, glycine-hydrogenated sodium bar, boric acid-hydrogenated sodium bar, oxalic acid-dimethyl sodium glycinate bar, hydrogen sulfate Sodium sulfonate bar, borax-sodium hydroxide bar, borax-sodium barium acid bark, sodium oxalate-basic acid bar, sub-acid sodium barium hydroxide bar, or other similar buffers. The above-mentioned water-based substrate is supplied at a temperature of 35 ~ 45C under agitation. The size and degree of the paper are applicable to the Ajia Family Standard (CNS) A 4 specifications (210 X 297 mm) ----------- ------- {----- 装 ------ # ------ 威 (Please read Note 6 on the back before writing this page) Central Bureau of Standards, Ministry of Economic Affairs B Printed by industrial and consumer cooperatives 81.9.20.000 A6 203556 B6 V. Description of invention (dead) (please read the note on the back & fill in this page) and leave it for 5 ~ 10 hours. The result of analyzing the filtrate of the fermentation product obtained by the badger by high-speed liquid chromatography (HPLC) shows that it does have the following components. In addition, HPLC instruments for analysis, such as the use of a detector (LC-9A, manufactured by Shimadzu Corporation), a chromatography column (length 3m), and a mobile device "Pseudo-use of acetonitrile solvent; there are conditions for measuring eel, column temperature 30t :, the flow rate is 1 Om 丨 / mi η. In the filtrate of the alcohol production product, myristic acid, palmitic acid, stearic acid, alginic acid, camellic acid, eicosanoic acid, dodecanenoic acid, myristyl acid, Myristyl dienoic acid, pentadecenoic acid, hexadecanoic acid, oleic acid, linoleic acid, linolenic acid, eicosenoic acid, eicosadienedioic acid, arachidonic acid, eicostrienoic acid Fatty acids such as dioxinol, crepanol acid, leucohexanoic acid, etc. exist. Also, in the same way, it is confirmed that lotus contains leucine, isoleucine, grade amino acid, methionine, cystine, phenylalanine, tyrosine, threonine, tryptophan , Amino acids such as proline, histidine, arginine, aspartate, alanine, glutamate, glycine, proline, serine, etc. Printed by the R Industry and Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs. The composition of the fermentation product mentioned above shows that the thinned product contains a variety of amino acids and fatty acids. Amino acids have the effect of supplying nutrients to capillaries; fatty acids have a vasodilator effect that can activate capillaries, and a protective effect to stabilize hair and make hair beautiful, and can be contained in hair follicles. -The decrease in the activity of the enzyme genigen can hinder the 5α-genigenase that inhibits hair follicle growth. In addition, these ingredients, or the synergistic effect of the ingredients, can promote hair growth and exert an excellent meal hair effect. In particular, 81.9.20,000 copies of this paper are produced in accordance with the Chinese National Standard (CNS) A4 specifications (210 X 297 mm). 203556 A6 The Central Bureau of Standards of the Ministry of Economic Affairs 8 Industrial and Consumer Cooperatives printed B6 V. Invention description (G) It contains thiamine acid, unsaturated fatty acid, etc., and the presence of some acids can provide excellent meal hair effect. In addition, there is a period of time that the fermented product produced by Qu's interaction with the substrate, in addition to the alcohols such as lipase and prion, will produce amylase, glutamine, Various enzymes, such as glutenase and pectinase, will decompose other components other than the fat and protein contained in the base when they interact with the substrate, and the results of the analysis by HPLC are willing to show, In addition to the components confirmed by analysis of the alcohol product broadcast from the mixture of lipase and prase, it contains vitamin A, vitamin Bi, vitamin B2, vitamin B6, vitamin B: 2, vitamin C, vitamin E, copper , Iron, calcium, fat, purine, degassed ribonucleic acid, ribonucleic acid, adenine, guanosine, uracil, cytosine, xanthine and other micro ft but a variety of ingredients. As mentioned above, some of the fermented products obtained from Qu sleep have a large amount of various ingredients. By multiplying the amount of these ingredients and the synergistic effect of the above-mentioned amino acids and fatty acids, the hair effect can be further improved. improve. In addition, when the hair-raising agent of the present invention is stored, it can be frozen or boiled. With this combination, the effect of meal hair liquid remains unchanged. Next, Η will explain the preparation method of the meal hair liquid of the present invention. In addition, the substrate, enzymes, and koji cocoon have been explained in the description section of the meal hair liquid, and will not be repeated here. There is a method for preparing the meal hair liquid of the present invention, and its stickiness is to make enzymes or aspergillus capable of decomposing plant proteins and plant lipids and water-based bases containing plant proteins and vegetable fats. -8 _ (Please read the notes on the back before writing this page &gt; • Binding · Order. Λ This paper is again applicable to China National Standard (CNS) A 4 specifications (210 X 297 mm> 81.9. 20,000 A6 B6 203556 V. Description of the invention ('7) First, the enzyme or aspergillus that can decompose vegetable protein sacs and vegetable lipids and the matrix containing vegetable protein S and vegetable lipids function, and the fermentation system is in aqueous conditions The enzymes that interact with the substrate, as mentioned above, are available for the use of lipids, prions, or in addition to some fat sugars, prions, and a variety of enzymes that produce starch syrups in raw mussels. Functional Qu Bin. In addition, you can add the fat brewing and prion separately to the base, or in the form of a mixture of fat yin and prion. Also, you can add other enzymes such as amylase to the mixture by poncho, and Improve the decomposition function, or carry out the decomposition of other components in the matrix. Furthermore, when enzymes such as lipase and prase interact with the base, the weight of each component can be reduced relative to the weight of the matrix 100 parts Add in a ratio of 0.1 parts. Also, because of the above Compared with brewed or prion, the decomposing power of the bacteria is weak, so the ratio of koji can be added to 1 per 100 parts of the substrate. There are fermenting conditions, and the rent is preferably 35 ~ 45t :, yes Two buffers, you can use kanacid-colibutine with a pH value of 6 ~ 11, potassium phosphite-potassium phosphate strips, sialic acid-Furana strips, kanacic acid-triaminomethane strips,》 acid Yimingshashi, boric acid monophosphate, strips, acid monoamine methyl propylene glycol strips, ammonium chloride monoamine strips, glycine monohydrogenated sodium strips, boric acid_hydrogenated europium strips, gallic acid monodimethylglycol Sodium amine acid strips, sodium hydrogen sulfate sodium citrate strips, borax monohydrogenated sodium strips, borax monohydrate sodium nitrate strips, sodium oxalate monohydrate sodium strips, sodium phosphite · • hydrogenated sodium strips or other similar It can also be used in the form of a mixture of these two kinds of male liquids, S. It can be used in the form of a mixture. Also, it is more than two hours, and the paper standard is suitable for Chinese poor families. Standard (CNS> A4 specifications (210 X 297 mm) ------------------_----- installed ------- 玎 --- --- ^ {Please read the note on the back ¾ Xiang Zaixuan wrote this page) The Central Bureau of Standards, Ministry of Economic Affairs, R-Industry and Consumer Cooperation Co., Ltd. produced 81.9.20,000 A6 B6 203556 V. Description of Invention (》), Tai can be 5 ~ 10 hours. After the anti-molecularization is over, there is lipase , Can be used to add certain pigments as indicators to confirm the placement of free fatty acids generated by the hydrolysis of esters, or to determine the amount of free fatty acids by salty titration using electrical methods. If it is difficult to confirm, the free enzyme can be quantitatively confirmed by colorimetry. There are saccharin, yin trihu colorimetric method, Yi Di, first make it weakly acidic, and then _ recognize that it is generated when heating ninhydrin The colorant of the coloring material quantitatively compares the amine group to confirm the amine group. When it is difficult to confirm this, the formaldehyde titration method of Saishan can also be used; this method is the phenomenon of employing the titration curve of α-amine group or imine group in the presence of formaldehyde. In addition, there are two pre-treatments such as sterilization. It is best to use an autoclave to filter the fermentation product obtained by the action of alcohol or _ surface and substrate under the above fermentation conditions, and add water or ethyl fermentation to the obtained From the filtrate, the hair growth liquid of the present invention can be obtained. For the use of B-shaped hair liquid, it can be applied directly, or mixed with shampoo, hair bug, hair liquid, hair tress, hair oil, or shampoo or conditioner. In addition, in this hair-raising liquid, Qi can include blood circulation promotion, metabolism promotion, anti-male hormone mask, anti-inflammatory, anti-allergic, thickener, anti -__, 澝 Lianggang, etc. Ingredients used in Mao. For example, vitamin E acetate, trimethoprim, minoxigil, di-gasification, dinitrogenase, benzoic acid α-amino-Lu, monodimethyl-T-hydroxybutyrate ethyl Ether, pentadecanoic acid glyceride, vitamin H, vinyl heterodiacid, very rich luluic acid, diphenyl hydrazine acid, boron uronic acid, -10-This paper is again applicable to the Chinese national standards ( CNS) A 4 specifications (210 X 297 mm) ------------------ {----- 装 ------ # ------ ^ {Please read the notes on the back of the book before writing this page) Printed by the Beigong Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 81.9.20,000 A6 203556_B6_ V. Description of invention (° j) (Please read the notes on the back first Please read this page again) Minisasanisik extract, mononitroguaiacol, Tangxinzi medicinal liquor, raw mulberry wine, hydrochloride, dibenzoylamine, D-pantothenic acid, water Lonic acid, lactic acid, sodium lactate, Pujiaxian to be extracted, menthol, etc. [Examples] Example 1 In this example, rice bran was used as a base, 1000 ml of water was added to 500 g of rice bran, and they were fully mixed, and then placed in a container suitable for flutes (glass or plastic) ), And then, add 5 g of aspergillus to it, and then add 3 g of hydrogen sulfonate as a buffer to adjust its pH. The container can be kept at about 451C by means of, for example, an electric heater, and it can be stirred at all times. Place it on the side while stirring, and place it on the side for a night. If the decomposition reaction caused by the fermentation is completed, the container will become muddy. Once this spot is found, the anti-matter will be filtered with cloth to obtain a translucent hair-raising liquid of about 1000ml. Printed by the Ministry of Economic Affairs, Central Standard, Yi Rong Consumer Cooperative Co., Ltd. According to the results of analysis of the filtrate of the fermentation product obtained by this method by high-speed liquid chromatography (HPLC), it does have the following ingredients. In addition, the HPLC instrument used for analysis, such as the use of a detector (LC-9A, manufactured by Shimadzu Corporation), a chromatography column (length 3 m), two you use acetonitrile solvent in the mobile layer; there are conditions for measuring , The column rent is 30t !, the flow rate is 1 〇m 丨 / m ί η. In the filtrate 100 · 8, it was confirmed to contain: leucine 350 mg, isoleucine 650 mg, lysine 390 mg, methionine 150 mg, cystine 160 mg, phenylalanine 370 mg, -11-81.9 .20,000 paper size is applicable to China National Standard (CNS) A4 specifications (210 X 297 mm) 203556 A6 B6_ V. Description of the invention ((〇) Tyrosine 250mg, Threonine 250mg, Tryptophan 55mg, Valamine Amino acid composition of acid 520mg, histidine 200mg, arginine 650 mg, aspartic acid 390mg, alanine 410mg, glutamic acid 7 10mg, glycine 300mg, proline 320mg, U amino acid 310mg , And including: endogenous acid 0.5 mg, palmitic acid 25.0 mg, stearic acid 1.5 mg, alginic acid 1.0 mg, mountain acid 0.5 mg, twenty four acid 5 mg, ten Hexasulfenoic acid 0.5mg, oleic acid 45.0mg, linoleic acid 48.5mg, linolenic acid 1.5mg, 20 [fatty acid 0.5mg fatty acid composition, and vitamin E 1 500 / ug, copper 150iu g , Beeping class 18 // g, etc. In addition, the filtrate obtained in Example 1 was used for the external effect of male and rat skin prisoners. The process was to use the obtained in Example 1. The filtrate is obtained by adding 20% ethyl acetate as preservative to 80% of the filtrate as a preservative, and the hair liquid is obtained. The meal hair liquid prepared according to this is used for the male scalp twice a day in an amount of about 5m1 each time. Compare the scalp-like scalp before using it and the scalp state after using it by 20 days, and compare it visually. In addition, for another male, you can use the scalp-like tendon before using it and the scalp-like tendon after using it for 40 days. Compare visually and determine the effect of each condition. Confirm that there are visible signs on the scalp of both men. In addition, the filtrate obtained in Example 1 was used, and 20% of ethyl yeast was added to 80% of the filtrate as the yeast Preservatives, and Badger Dessert Hair Liquid-12- The scale of the table paper is in accordance with the Chinese standard (CNS> A 4 specifications (210 X 297 mm) ----------------- -1 ----- 装 ------ # ------ Λ &lt; please read ¾ the notes on the back and then write the reference κ &gt; Printed by S Industry and Consumer Cooperative, Central Bureau of Standards, Ministry of Economic Affairs 81.9.20,000 S03556 A6 B6__ Fifth, the description of the invention (丨 丨). The hair nourishing liquid prepared according to this will be used for the external application of about 0.5m1 each time The skin of a rat, and the meal hair liquid was not used on the other paper. After 20 days, the skin of the rat with the hair liquid was used and the skin of the rat without the hair liquid was visually observed. For comparison, in addition, two other rats were used, and the skin state of the rats 40 days after using the meal hair liquid and the skin state of the mice not using the hair liquid were visually compared. As a result, the mice that did not use the hair-growing liquid did not produce hair, but the a. Of the hair-growing liquid for external use all had hair-growing phenomenon: In addition, the filtrate obtained in Example 1 was further treated by other methods. Try its external effects. Use the animal Your female golden retriever vole, brewing weight is 1 lOg ~ 120g. Experimental method ⑴ The part centering on the diaphragm organs, the hair will be braked with a shaver (4 ~ 5 times a day). (2) Simultaneously apply 5wg (15W acetone) propionate propionate and 15wl filtrate (hereinafter referred to as STY-9201) on the abdomen organ on the left side. Right indium is not subject to treatment. Experimental group 1: Group 9: Nine sterone propionate 5 «, Group 2: Nine sterone propionate period 5« 8, obtained by adding STY-P201 stock solution, Group 3: In 5ug of propionate xanthone sterone, add STY — 920 1 stock solution, 3 groups: in 5Wg of propionate army nine sterol surface, add STY — 920 1 concentrated 2 times solution, 4 groups: in 5ug monopropionate propionate, add commercially available -13- Ben · Paper is once again universally applicable to the Chinese West National Standard (CNS) Grade A 4 (<210 X 297 mm) ~~ 81.9.20 〇〇〇 ------------- ----- {----- 装 ------. ΤΓ ------ β (please write IKIift backnote &amp; matters before writing) Jiabu Gong, Central Standard, Ministry of Economic Affairs Printed by the consumer cooperative A6 B6 203556 V. Description of the invention (α) The hair-raising liquid (purchased by Zidian, manufactured by Zhongfang Co., Ltd.). During the experiment period, from May 8th, Heisei 4 (1992) to May 18th, 2005, except for the 17th and 24th, use it once a day on the 26th The organ is placed in 10% formalin solution. Judgment method A tilted gland is formed into a cut in the center, and HE specimens are made from the mine surface on the surface. Then, place the micrometer into the eye shovel, and expand the area of a fat gland by the number of eyes measured with a 100-fold enlargement. The fat glands are targeted at those whose fur passes through the center. For each corpse, the left and right knees were counted to their 10® positions, and the average value was used to compare the groups. The results are shown in Table 1. Female T shouted STY-9201 XI Disposal group 25.6 ± 5.2 20.fi soil 2.2 ** Zhu Chu® group 5.3 soil Q.8 4.7 ± 0.7 Disposal group · Nowhere collar group 20.3 ± 5.5 16.0 ± 2, 2 STY- 9201 X 2 Purple electricity changed to the hidden group 20.1 ± 2.5 ** 17.7 ± 2.7 ** Unsettled S group 5,5 soil 1.0 4.7 ± 0.6 · lit group + un · disposal group 14.δ ± 3 · 2 * 13.1 ± 3.0 »* -14- This paper is again applicable to China's a standard (CNS) A 4 specifications (210 X 297 mm) -installed ------. 玎 ------ β {please read first Note on the back * Matters will be reprinted on this page) Printed by R1.9 Consumer Cooperative, Central Standards Bureau, Ministry of Economic Affairs 81.9.20,000 A6 B6 203556 V. Description of invention (β)

Dunnett's T test (relative control: Μ &lt; 〇. 〇1 〇. 05) the average soil S. D with the control group as the control, the "set group, use the value without * set group correction, use Dunnett's multiple The comparison shows that the results of the comparison of the groups show that STY — 920 1 is twice as dense, P &lt; 〇. 〇5 is considered to be an intentional difference, and, for Zixiao, P &lt; 0. 01 is considered to be intentional The difference. From the above experimental results, it can be seen that the double concentrated formulation of STY-920 1 is compared with the control group, and at Ρ &lt; 0.05, the intentional fat gland reducing effect is determined, which shows that the thinning product of this example The filtrate (STY-9201) has the following facts. ⑴STY—9201 will hinder the combination of testosterone and its receptor. ⑵The path that is completely unheard of according to (1) also has the effect of shrinking the fat glands. ⑶ Block 5α-reductase. In addition, according to the above experimental results, the role of testosterone _ and its combination of blocking effect and hair raising effect, the role in the occurrence and growth of hair nipple hair (pig disease diagnosis and treatment: 1 1 &lt;12); 1020 ~ 10 25, 1985, the author Katsuoka Tosho)). In addition, the hysteresis of the fat gland's small effect and hair-raising effect is described in the generation mechanism of the adult hair removal solution and its treatment method. The application is 5 α -Tongyuan enzyme inactivation Lang® bed application (Japan Beauty Journal of Surgery, Vol. 22, No. 2 (February 1, 983). Secondly, H is the filtrate of the fermentation product obtained in Example 1 (below -15- This paper is again General Chinese National Standard (CNS) A 4 specifications (210 X 297 mm) ------------------ {----- 装 ------ # ------ β { Please read the notes on the back before filling in this page) 81.9.20,000 203556 A6 _B6_ printed by the Employees ’Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs 5. Description of the invention (丨 屮) (please read &quot; read the notes on the back &amp; items first (Write this page) Call it STY — 920 1), conduct skin contact sensitivity test and skin irritability test. The details are as follows. Regarding the test materials for the skin contact sensitivity test (1) Test material 1): Contains STY-9201 (50 ml), ethanol (40 ml), purified water (10 ml), perfume (Shield w-20 / HB ) (0 · 5m 1), soluble solubilizer (the original 4 0 9 P) (1.0ml). Test material 2): Contains STY-9201 (50 ml), ethyl yeast (40 ml), and purified water (10 ml). (No fragrance) ⑵ Test animals Take 20 Hartley female marmots (heavy weight 258 ~ 3 1 2g) for the test. (3) Feeding conditions: Animals printed by WK Industrial and Consumer Cooperatives, Central Bureau of Standards, Ministry of Economic Affairs, 22 degrees in Shanghai, 2t :, relative S degrees, 55 ± 10%, ventilation frequency 3 to 5 times / hour (All flash air method), lighting time 12 hours / day (stick the lamp at 7 o'clock in the afternoon, "lamp" at 7 o'clock in the afternoon), etc. under the conditions of constant ffi and constant humidity, each is accommodated in an aluminum scythe with 5 horses, and hang it on the tandem washing rack Feeding in Taichung. ⑷Feed and beverage water solid feed RC-4 (Oriental Yeast Industry Co., Ltd.) and beverage water (water supply from Mihara-machi tap water with automatic water supply device) You are free to take -1 6-81 * 9.20,000 This paper size is applicable to Chinese national standards (CNS) A4 specifications (210 X 297 mm) A6 B6 203556 V. Description of invention (IP). (5) The test drug 2,4-dinitrotriphenylbenzene (DNCB) you use Wako Pure Drug test drug waiting grade; olive oil, white petrolatum and physiological food for injection, and Japanese prescriptions for Japanese medicine are used; and , Freund's Coeplete Adjuvant (FCA) you use Difco products. Test method ⑴ Piru one stimulus preparatory test is based on the maximum non-effect amount of the test material ⑴ which is clear and suitable for the soil, and the 25, 10 and 5w / w% soft capital is adjusted when used (base surface: white Vaseline), using 0.2g of pre-cut ventral skin It, respectively, for 24 hours of occlusion and spreading. In addition, two B animals were used at each of the above concentrations M, at 24, 48, and 72 hours, according to the following

Draize benchmark to determine the symptoms of Piru. Evaluation of Piru anti-codling A. Formation of dry erythema scab formation -------------------- {----- 装 ------ ^ ----- -0 (Please read the notes on the back before writing this page) The Ministry of Economic Affairs, Central Bureau of Standards, Employee Consumer Cooperative Indica has no erythema ... 0 Almost no erythema ... 1 Unable to confirm the level of erythema ... 2 Moderate erythema ... 3 Sleeping weight The erythema ... 4 The maximum erythema "· 4 B. Floating poncho formation -17--paper scale is applicable to China National Standard (CNS) A 4 specifications (210 X 297 public goods) 81.9.20,000 203556

A6 BG 5. Description of the invention (丨 G) No puffiness ... 〇 Almost no puffiness ... 1 Unable to confirm the degree of puffiness ... 2 Moderate degree of puffiness ... 3 Joint weight puffiness ... 4 Maximum puffiness ... 4 Group constitutes the Ministry of Economy This sample STY-9201 was printed by the Staff Cooperative of the Central Bureau of Standards and used in a group of 10. According to the preliminary test results, the final concentration was set to 2. Skin 25% Qi Minqun, and attached STY — 920 1 of 5w / w96 ointment on the right ventral side, and attached STY-920 1 (without fragrance) 5 \ //% soft threat on the left ventral side for induced treatment . In addition, for the positive control DNCB, using 1 group of 5 mice, under the conditions of intradermal sensitization concentration of 0.1%, 1.0% attachment, and 0.01% induced conditions, the rate of detection of sensitization is 100% Therefore, using this condition 0 again, there is a period of negative control, ready to use the sensitized group of physiological diet, take 1 group of 5 animals. Based on the above results, coal is shown in Table 2 below. -18- ------------------ ί ----- install ------ ΤΓ ------ 0 {Please read the notes on the back first Details of this matter are written on this page.) This paper scale is applicable to 8 specifications (CNS) A4 specifications (210 X 297 mm &gt; 81.9.20,000 203556 A6 B6. V. Invention description (q) [Table 2] Group Foam sensitivity concentration (W / W%) Intradermal patch STY-9201 2.5 25.0 STY-9 2 0 1 (no fragrance) DN CB 0.1 1.0 A group of physiological foods cause mame (W / W%) patch STY -9201 5. 0 STY-9201 (without fragrance) 5. 0 D NCB 0.01 Physiological food is water- (Please read the notes on the back before writing the clothing page) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs Number of animals STY-9201 i 〇 (right abdomen) STY— 9 2 0 1 (no fragrance) 1 〇 (left abdomen cue) DNCB 5 Physiological food water 5 -19- This paper is again applicable to the Chinese National Standard (CNS) A4 specifications (210 X 297 mm) 81.9.20,000 Printed by the Central Ministry of Economic Affairs, Standard Employee Consumer Cooperatives 203556 V. Invention description (Μ) *: STY-920 1 and DNCB are provided with Vaseline base ointment 。 Negative control physiological dietary solution • For use This pharmacy uses physiological water for injection. Cleaving of the sample 1> The preparation of the emulsifier of the sample for intradermal sensitization is STY-920 1, in the volume of the FCA1 instrument where the final concentration is 5%, or, DNCB , Mix and dissolve it to a final concentration of 0.2% F CA1 healthy volume by using Mizuo milk powder and sonic pulverizer, and add physiological food for injection "1 volume of water, according to the general method, prepared at the time of use 2) The preparation of intradermal sensitizing sample solution is STY-9201 and DNCB, which are dissolved in medicinal oil prepared by the pharmacy of Meizhizhi, making them 2.5% and 0, respectively. . 1%, to be adjusted when used. 3) The cracking of the sample ointment used for the sensitive test will weigh STY — 920 1 to form an amount of 2596. On the glass * plate, it will be fully trained with an appropriate amount of white petrolatum. When used, it will crack into ointment. In addition, regarding DNCB, the amount of DNCB is pseudo-formed to form 1.0%. In the beaker, it is heated to about 60 on the one side, and mixed with white petrolatum on the one side to melt it, making it exothermic and made in use. ointment. 4) STY-920 1 and STY-920 1 (without perfume) -20- ------------------ (---- -Install ------ ir ------ let &lt; please note the item on the back of Mift first and then write this page) The size of clothing paper is applicable to China National Standard (CNS> A4 specifications (210 X 297mm> 81.9.20.000 203556 V. Description of the invention ((j) (please read the notes on the back &amp; matters and write this page on iodine), make it 5%, and DNCB make it 0.01% , It is prepared in the same way as in 3). Use the sensitization method to cut the shoulder blade of the male orange rat by 4X6 cm, and inject 0.5ml of the sample into the skin at the position of Sanyu. 1) The complete supplement of Flanders 2) Only the pulp is dissolved or mixed with olive oil. 3) Mix or dissolve with the complete supplement of Flanders to form w / o emulsion test buns. After 1 week of intradermal injection, cut the same part of the hair and apply 10% sodium lauryl sulfate (Vaseline base) to the mandarin fish, but not DNCB, and then take the check (Vaseline base) 0 . 25%, unfold it on a 2X4 cm filter paper, and attach it for 4 to 8 hours for additional testing. The induction method was encountered twice after the final sensitization. On the opposite part of the other side of the side, STY-9 2 0 1 (without fragrance), the hair was cut 5x5cm, and the wound band was 0. 5 inch (Torii Hibiscus) ), Coated and inspected. 2g. Printed by the Rigong Consumer Cooperative of the Central Bureau of Economic Affairs of the Ministry of Economic Affairs. The patch is blocked for 24 hours. Judgment criteria After 24, 48 and 72 hours, the criteria shown in Table 3 are used for judgment. Test Results-21- This paper is again in accordance with the Chinese Standard (CNS) A4 specifications (210 X 297 mm) 81.9.20,000 203556 A6 B6 V. Description of the invention (AO) [Table 3] Evaluation criteria 0 Visual Upper back change 1 Shoe skin, erythema due to sex 2 Moderate chronic erythema 3 Intensity erythema and floating a (please read the notes on the back before writing this page) The test result will be tested material STY— Groundhog skin 920 1 _ sensitization, with DNCB as a positive control, according to Maxiaization method to be discussed. Yidi, prior to this test, based on the purpose of knowing the maximum inactive amount of the tested sample, conduct a preparatory test for the excitement of the groundhog skin *, and according to the result, conduct a final concentration of 2.5 w / w% After the dermal sensitization treatment under the skin, the patch with the maximum non-effect concentration (25w / w%), and using the left and right ventral parts, carry out 5w from STY-920 1 and STY-9 20 1 (without fragrance) / w% of the induced disposal of petroleum jelly, except for the employee consumption cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs, other than India and Li, and the positive control DNCB, the same treatment was carried out to verify the concentration of various documents. (1) One stimulus preparatory test for skins For the purpose of understanding the minimum and maximum effects of STY-920 1, 2 animals were used for 1 degree of ointment, and each sample was subjected to 24 hours of occlusion and patching. In addition, the judgment is based on the Dray ’s benchmark, and the whole case is -22- 81.9.20.000. The paper is again applicable to the Chinese National Standard (CNS) A 4 specifications (210 X 297 mm) 203556 A6 B6 V. Description of the invention (〗丨) 2 It is used as a minimum estimate for your weight,% shows 5 places 2 4 If you use it in the table 丨 as swollen without puffiness and the most spot, red on the shape of the shape% not 5 {Please read ¾ back Matters needing attention to write this page) The paper printed by the Staff Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs again applies the Chinese National Standard (CNS) A 4 specifications (210 X 297 mm) 81.9.20,000 203556 V. Description of invention () 1) 〔Table 4〕 〔心 斗〕 Moss time point 2 4 small β | · Swab dystrophy animal No. 1 豳 WNo. 2 erythema floating << erythema floating taboo 2 5% 0 0 0 0 10% 0 0 0 0 5% 0 0 0 0:, H cold point sample test 4 8 hours animal No. 1 _WNo. 2 erythema floating Ιί erythema floating a 2 5% 0 0 0 Q 1 0% 0 0 0 0 5% 0 0 0 0 (please First «15 Note on the back of the item * and then write this page) 丨 Install. Order ... Spin! The concentration of the test sample printed by the employee consumer cooperative of the Central Standards Bureau of the Ministry of Economic Affairs at the time of inspection 7 2 / Bu Shi Animal No. 1 Animal N 0. 2 erythema floating sleep erythema edema 2 5% 0 0 0 0 1 0% 0 0 0 0 5% 0 0 0 0 -24- This paper is again applicable to the Chinese National Standard (CNS> A4 specifications (210 X 297 mm) 81.9.20,000 203556 A6 B6_ V. Invention description (Μ) (Please read the notes on the back first Matters will be written on this page) ⑵S TY — 9 2 Ο The root of 1》 Maxiiazation method of skin sensitization test results. First, 5% Vaseline soft buds will be attached to the cloth for 24 hours. At 24, 48 and 72 hours, the naked eye was observed, and the judgment results are shown in Table 5. As shown in the table, when 5% Vaseline ointment of STY-9201 was added, at 24 hours, all cases were accompanied by mild Erythema skin-pig reaction (100% sex rate, average rating: 1.0), and then gradually lightened up, and at 72 hours, gold cases were found to recover. On the other hand, STY — 920 1 ( No fragrance) of 5% Vaseline ointment, and no skin reaction was found (10 cases) at 24, 48, 72 hours after the cause (The incidence rate is 0%, the average rating: 0.0). Therefore, under the test conditions, the tested sample ST Ya-9201 has weak sensitization, and its sensitive skin-pigment reaction is designated as Okay, but ST Y-920 1 (without fragrance) has no sensitization at all. Therefore, it is presumed that the material contained in STY-920 1 is the substance causing the sensitization. Namely, it is presumed to be an antigen. Printed by the Ministry of Economic Affairs, Central Bureau of Industry and Commerce, 8 Industrial Consumer Cooperatives (3) The result of the skin sensitivity test based on the Maxinization method of the positive control DNCB. Attached 24 hours, after the cause, the naked eye was observed at 24, 48, and 72 hours, and the judgment results are shown in Table 5. As shown in the table, at 24 hours after the cause, meteorological anti- 25- 81,9.20,000 This paper scale is applicable to China National Standard (CNS> A4 specifications (210 X 297 g *)) 203556 A6 B6_ V. Description of the invention (4) 醮 (Viscosity 2: 2 examples, evaluation 3 : 3 cases, average rating »2. 6). In addition, there were also cases of remission at 48 and 72 hours. In the final observation, the skin remained in the whole case. Symptoms: Therefore, it is extremely powerful for the groundhog skin of DNC B positively, and the degree of sensitivity of skin reaction is also very strong. ⑷Maximization method based on the negative control physiological diet The results of the skin sensitivity test were first attached to the caused patch with white petrolatum ointment for 24 hours. After the cause, the naked eye was observed at 24, 48 and 72 hours. The determination results are shown in Table 5 and Table 6. As shown in the table, at 24, 48 and 72 hours after initiation, the sensitization of the groundhog skink to the negative control physiologic food (white petrolatum) is under the test conditions because the viscosity is M0 ”. It is presumed that it has not occurred (please read the note on the back &amp; matters and write this page again)> Printed by the delta industrial consumer cooperative of the Central Standards Bureau of the Ministry of Economic Affairs-26- This paper is again applicable to the Chinese Standards (CNS) A4 Specifications (210 X 297 mm) 81.9.20,000 A6 203556_bo 5. Description of the invention (υγ) [Table 5] 0 0 0 0 0 0 0 0 0 0 0 (Please read the notes on the back and write this page) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ^ gte Zhao Zhao aluminum lsA ,, s 畤 0 I 0 0 0 0 0 0 0 0 0 0 0-installed. La-ir ^ z 0 0 0 0 0 0 0 0 0 0 order. • ozs 0 00 L 9ε τ 00 L 9 9 ZO

S0S Printed by e-consumer cooperative of Central Standards Bureau of Ministry of Economics ¢ ¾ Λ

ϊ 0Μ6 —Λ 1 S 27-

I 0Ε6 丨 Λ 1 S This paper is again applicable to the Chinese National Standard (CNS) A 4 specifications (210 X 297 mm) 81.9.20.000 203556 A6 B6 V. Description of the invention (爻 U 【Table 6】 Ministry of Economy Central Standard Min.i Employee dismissal, co-operatives printed the largest evaluation value during the 18 inspection period CO CO C〇 (Ν Μ ooo 〇〇 皮 痗 反 S Evaluation point 7 2 small θ | f- * CNJ * -iooooo 〆00 ΟΊ CM (Μ Ν — Ο Ο o 〇〇2 4 small 0f CO CO CO cv | oooo 〇 Qinwu No. h co 1 in ^ cm 〇〇tj * m concentration caused by patch% oo 1 green concentration% patch t t intradermal o 1 sample name positive photo DNC β Negative Photograph Ti Ti (Fl Shihlin) 28 This paper is again applicable to China National Standard (CNS) A 4 specifications (210 X 297 g *) 81.9.20,000 ------------ ------ {----- 装 ------. 玎 ------ β (please read ¾ Note A on the back and then write this page) Central Bureau of Standards β A6 20,3556-^-printed by the Industrial and Consumer Cooperatives V. Description of the invention () 1) From the above test results, it can be known that the filtrate of the fermentation product of the present invention (s TY — 920 1) When used alone, it is confirmed that there is no skin sensitization In addition, although the filtrate of the fermentation product of the present invention that contains the material has some skin sensitization, when it is attached to the skin at a low concentration set, there are several stages of conditions, Therefore, Piru's sensitization can be considered as a range that can be ignored. Piru's primary stimulation test test material (1) Test sample 1): Contains STY-9201 (50 ml), ethanol (40 ml), refined Water (10 ml), spices (Shield W-20 / HB) (0.5 m 1), solubilization (Emma original 4 0 9 p) (1.0 ml) o tested sample 2): Contains STY — 9201, Ethanol (40 ml), grain cracking water (10 ml). (No fragrance) ⑵ Test animals 6 white rabbits (2. 5 months old) healthy rabbits (weight: 2.5 kg) were used for the test. (3) Feeding conditions: the temperature of the animals at 22 soil 2¾, the relative temperature of 55 ± 1 0968, the number of air exchanges (Allflash air) of 3 to 5 times / hour, 12 hours / day (7 sticky lamps before noon, 7 turns off in the afternoon) Xuan) under the constant temperature and constant set of blocks, one by one contained in a cracked aluminum cage, then -2 9- This paper is again applicable to the Chinese In-house Standard (CNS) A 4 specification (210 X 297 mm) 81.9.20.00 «------------------ {----- install ------ 玎 ------ spot (please first (Read the note on the back & matters and then write the clothing page) 203556 A6 B6_ Fifth, the invention description (β) and hang it on the squash-type washing rack for feeding. ⑷Feed and drink water Solid feed RC-4 (Oriental Yeast X Co., Ltd.) and drink water (water supplied from Mihara-cho's tap water by an automatic water supply device) are available for free intake. Test Method This test method combines the method of the EPA Guidelines (1978, Job State Registration) with the method of acute skin irritation / corrosion test proposed in the 1984 OCD Guidelines, in the following manner. (1) Split of the test sample The test sample is used as it is by the person who obtained it. ⑵Group structure The group structure is implemented with 6 animals in a group according to the group structure shown in Table 7 below. ------------------,-· ----- 装 ------ # ------ Wei (please note on the back & 1¾¾ first) Matters will be written on this page) Printed by the Employee Consumer Cooperative of the Central Bureau of Standardization of the Ministry of Economic Affairs -30 · This paper is again applicable to China's a standard (CNS> A4 specifications (210 X 297 mm) 81.9.20,000 2〇35 stations -V. Description of the invention (i ° |) [Table 7] A6 B6 Group distinguishing parts N 〇. Applicable parts I Jianqin Piyong 1 1 ~ 6 Intended material 1 II Damaged skin 1 1 ~ 6 Intended material 1 III Healthy skin 2 1 ~ 6 Test sample 2 IV Injured skin 2 1 ~ 6 Test sample 2 Printed by the Ministry of Economic Affairs Central Standards Bureau B Gongxiao F Cooperative ⑶Operating method Take healthy white rabbits 6 in advance The side WP is fully shaved. Among them, the left abdomen detail ventral side and the right complex side head americium image remain intact (healthy skin *), and the left skin side head side and right ventral side skin system are used • After the 18G injection needle is given a wound on the cornea (injury to the skin), use the test tube. That is, apply a blocking paste with a diameter of 1 inch (made by Torii Pharmaceuticals) impregnated with a 500wm skeleton to lock the patch. Then And covered with Hold the cloth to make the contact between the sample and the skink more reliable. After 4 hours of airtight patching, remove the specimen and observe the skink's symptoms after 1 hour. Then, place the animal as it is, at 24, 48 And 7 and 2 hours later to observe again. -31- ------------------ {----- install ------ order ------ line / I (Please read the note A on the back and then 5TW page) This paper is again applicable to the Chinese home standard (CNS> A 4 specifications (210 X 297 mm) 81.9.20,000 A6 B6 SQ355.6- V. Description of the invention (eg) (please read the precautions before proceeding to the AfW page). There are evaluations of the symptoms of pelvic skin, the health skin and the damaged skin are evaluated separately, and visual inspection is performed on the basis of Draize (Table 8) To determine the two items of erythema, scab formation and float formation, in addition, to evaluate the skin reaction based on Campbell's criteria (Table 10). In addition, after the sample is removed, 24, 48 and 72 hours, according to Say the inflammation index or use other methods to evaluate the irritancy of healthy skin and damaged skin according to the P.I.R value. For the inflammation index, you add the total value of the healthy skin and the damaged skin to the total value of the damaged skin. Divide it by the number of animals 6. The PI R is the sum of "Kangpiru stick and injured Piru stick 24 hours and 7 2 hours, divide it by 2 and divide by 6 animals" Value ⑷ Observation of the general state During the test period from when the sample is used until the end of the observation, the state of the animal is covered with hair, eyes, ears, # fecal dirt, genital, anal fecal dirt, whether there is diarrhea, etc. Observation of general status and food tweezability Printed by the Ministry of Economic Affairs, Central Bureau of Standardization and Employee's Consumer Cooperatives -32- This paper A degree applies to the troubled a family standard (CNS> A 4 specifications (210 X 297 mm) 81.9.20,000 203556 A6 _B6 5. Description of the invention (3 丨) (Please read the precautions on the back before writing this page) [Table 8] According to Draize ’s evaluation criteria for skin irritation ⑴ erythema and scab formation without erythema .. ............... 0 Very mild erythema (the degree to which it was finally confirmed) 1 Obvious erythema ......... ............................ 2 moderate to strong erythema ............... ............... 3 Dark red intensity erythema with light bursa formation ( Accessibility obstacles) ......... 4 The total number of sticks is the largest ... 4 ⑵ Puffiness forming mmm .................... ......................... 〇Extremely mild puffiness (the degree to which it was finally confirmed) 1 Mingying buoyancy Teng (can be clearly distinguished from Zhou Zuo) 2 Moderate degree of puffiness (floating above lmm) ............ 3 Strength puffiness (floating above 1mm, expanding towards Zhouyuan)… 4 knots The number of stickies is the largest ... 4 Printed by the Staff Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs -33- This paper is again applicable to the Chinese National Standard (CNS) A 4 specifications (210 X 297 mm) 81.9.20,000 203556 A6 B6 V. Description of the invention (U) [Table 9] The criterion for determining the irritability. The safety plaque is divided into the primary irritability coefficient (P.I.D. Weak irritant 2 Medium irritant 2 ^ 5 Strong irritant 5 ~ ---- -------------- 1 ---- installed -------- ordered ------ line I (please read the notes on the back before writing Page) Printed by the Staff Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs -34- This paper is again applicable to the Chinese Western Standard (CNS> A4 specifications (210 X 297 mm> 81.9.20,000 203556 A6 B6 V. Invention description (W) [Table 1 0] Based on the evaluation criteria of Caepbell's skin

(Please read the notes on the back &amp; matters before writing a wooden page) Printed by the Ministry of Economic Affairs Central Standards Bureau tK Industrial and Consumer Cooperatives -35- This paper is again applicable to China National Standards (CNS) A 4 specifications (210 X 297 mm ) 81.9.20,000 203556 A6 B6 5. Description of the invention (&quot; Η)

----------------------- installed ------, 玎------- flag (please read the notes on the back first) Write this page again) The paper standard printed by the Staff Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs is applicable to the Chinese National Standard (CNS) A 4 specifications (210 X 297 mm) 81.9.20,000 203556 a6 B6 V. Invention description (\ r) 〔Table 1 1〕 Classification of the degree of irritation. Healthy skins are wounded. Skin irritation levels are 0 ~ 0.9 0 ~ 0 · 9. Human skins can be touched. 1 ~ 1.9 For healthy skins, for skin wounds. Exposure may be 2 to 4 after prevention. For healthy skin, contact with damaged skin may be avoided. 1 ～ 1. 9 1 ～ 1.9 After prevention, both health and damaged skin may be touched. 2 to 4 After prevention, Healthy skins can be touched, but it is appropriate to avoid contact with damaged skins 2 ~ 4 2 ~ 4 It is suitable for healthy and damaged skins to avoid bowel (please read the notes on the back &amp; matters before filling in this Page) The Ministry of Economic Affairs Central Standards Bureau employee consumer cooperative printed the test results of the test sample STY_9201, which was a stimulus test, pretending to be healthy and saving male animals Krupp skin, occluded patch for 4 hours, the patch was removed after 1.2 and 72 8 4,4 Zhi small, and by Praize

Based on Campbell's criteria, the evaluation of skin symptoms is made, and there is a result of mollusk evaluation. -37- This paper is again applicable to China National Standards (CNS) A 4 specifications (210 X 297 mm) 81.9.20 000 203556 A6 B6 V. Invention Explanation (U) STY -920 1 is shown in Tables 12 and 13, and STY — 92 0 1 (without fragrance) is shown in Tables 14 and 15. (Please read the note on the back-matters before writing on this page) Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs-3 8 _ This paper K degree is applicable to China National Standard (CNS) A 4 specifications (210 X 297 mm) 81.9.20,000 A6 _B6 V. Description of the invention (") [Table 1 2] Evaluation based on the Draize benchmark but the rabbit skin)! Building to the branch 1 48 7Z 1 72 No. No., Bu Bf / Kflf ㈣ / W f Buf, Ka | 4 time Ί'βΙ I 0 ϋ 0 0 0 0 0 0 2 2 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 4 mine 0 0 0 0 0 0 0 0 0 碓 5 0 0 0 0 0 0 0 0 6 0 0 0 0 ϋ 0 0 ϋ 1 0 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 4 11 injury 0 0 0 0 0 0 0 0 5 0 0 0 0 0 0 0 0 G 1 0 0 0 ϋ 0 0 0 (Please read the notes on the back of Qi before writing this page) Printed by the Employees Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs [ Table 1 3] Evaluation based on the Campbell benchmark V2 Well-being and quasi-employment 1 Q ο 0 0 0. 0 0-0 2 0 0 0 ϋ 0. 0 0. 0 3 0 ϋ 0 ϋ 0. 0 0.0 4 ϋ ϋ 0 0 0.0 0- 0 5 0 0 ϋ 0 0.0 0.0 6 0 1 ϋ 0 0.0 0.5 Ρ. I. \ ι 0.00 0. 08 -39- Original paper And Degree of Chinese National Standard (CNS) A 4 size (210 X 297 mm &gt; 81.9.20.000 203556 A6

BG 5. Description of the invention 〇 ") [Table 1 4] Evaluation based on the Draize benchmark Ten% k No. Skin / Λ: 'ίΛ;! ΛΚθ | small inch α), β | 7 ^ / 1, Shi Guang Μ 吾 小 η 1 ϋ ϋ υ — υ 0 0 0 ϋ 2 0 ϋ 0 0 0 ϋ 0 0 3 0 0 ϋ ο 0 0 0 0 Λ weave 0 0 υ 0 0 ϋ 0 ϋ 5 ο 0 0 ϋ · 0 0 ϋ 0 ϋ 0 0 ο υ 0 0 0 0 1 0 0 0 0 0 0 ϋ 0 2 0 ϋ 0 0 υ 0 0 0 3 0 D 0 0 0 0 0 0 4. Ύβ / 4 0 0 0 ϋ 0 0 0 0 5 0 ϋ 0 0 υ 0 0 0 G 1 0 υ 0 0 0 0 0 〔Table 1 5〕 Evaluation according to the Caupbell benchmark IU- + β | 4 Shi Jian 4 Lai Shang ίWei iitA 童 味 丄 0 0 0 υ 0. 0 0. 0 2 0 ϋ 0 0 0.0 0.0 3 0 0 0 0 0.0 0.U 4 0 ϋ 0 0 0.0 0. U 5 0 0 0 0 0.0 0.0 • ϋ 0 1 0 α ϋ.ϋ tl . 5 Γ. I. \ ι. Ϋ. 00 0. 08 (please read the notes on the back of ^ first and then write this page). Install · β.! Printed Leather by Employees Consumer Cooperative of Central Bureau of Standards, Ministry of Economic Affairs Evaluation a) When STY-9201 is used in healthy skinned persons: During the observation period of 1 hour to 72 hours after the removal of the sample, no skinned reaction occurred in all cases. -40- This paper is again applicable to the Chinese National Standard (CNS) A4 specifications (210 X 297 mm) 81.9.20.000 Printed by the Employee Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs V. Description of the invention (1) When it is used to damage the skin : About 1 hour after the removal of the sample, only one case (animal N 0.6) observed redness with a viscosity of 1, and the symptoms disappeared at the 24th hour. Later, during the observation period up to 72 hours, none of the other Piru anti-S occurred in all cases. b) When using STY-9201 (without fragrance) on healthy skins: During the inspection period of about 1 hour to 72 hours after the removal of the sample, no skin resurgence of Jinli's skin occurred. When used to injure the skin: 1 hour after the removal of the sample, only 1 case (Animal No. 6) «Redness was found in the evaluation point 1, and the symptoms disappeared at the 24th hour. After that, in the two observation periods up to 72 hours, other skin-reactions did not occur in the gold case. Irritation evaluation a) For STY-9201, the PI · R obtained from the above results is used as a healthy skin, the value is "0.0〇". In addition, it is used for damaged skin, the value It is "0.08", and they are all classified as "no stimulus" in terms of security. In addition, if there is a degree of stimulus in practical use, Misaki is classified as "non-irritant edible" by the classification of Campbell and others who can be contacted with "Health and Injury Skin" and is safe. B) As for STY — 9201 (without material) When the PI R obtained from the above results is used in a healthy skin, the value is “0. 00”》 In addition, when it is used in »wound skin_, the value is“ 0 · 08 ", Are all classified as" non-irritating "in the safety plaque. -4 1- Wood paper ruggedness applies to the CNS Standard A 4 specifications (210 X 297 mm) 81.9.20.000 (please read the notes on the back and then write 1)-Pack · Order · A6 B6 (13556 V. Description of Invention (UD) In addition, there are two practically irritating degrees, which are equivalent to "can be used for health and injury *, Anjin J's Campbell and others are classified as plaques. Irritation substances. The general state of the test results from the date of use of the sample to the end of the observation (72th hour), every day is the case of the covered hair, the feces of the eyes, ears, and nose. Observe the general status and food intake, and the results are willing to indicate that no abnormalities are worth mentioning on any observation day. During the inspection, the health status is good. Root "PI R classification of Coipbell et al. : Healthy skin PIR 0 ~ 0.9 Non-irritating, safe for contact with healthy skin. Damage skin STP.IR 0 ~ 0.9 Non-toxic for damaged skin cells, accessible. Can be connected with human skin »〇According to this Test results, classified according to the above, STY-920 1 and ST Y — 9201 (no fragrance) You are not corrosive; and according to the irritability degree of Cambell et al., Confirm that the yoke is equivalent to “for both health and damage to the skin, the safety j is classified. In addition, in this regard The cracking process of Example 1 was repeated twice (the second and third times) to obtain a filtrate having the composition shown in Table 16 below. From this table, it can be seen that the reproducibility of the manufacturing method of the present invention is not in doubt -42- This paper is again suitable for China B Jialin (CNS> A 4 specifications (210 X 297 mm) ------------------- J .-- --Installation ------ ΪΓ ------ End- (please read the back of the first; read the clothing page after reading the matter >> Printed by the Ministry of Economy Central Standard Staff and Consumer Cooperatives 81.9.20.000 6553 ο 2

AB V. Description of Invention On) Printed by Vtb TO o〇f U1 11 ί M-ru ro g CO err 〇〇 雄 m κλ on Γν5 〇1 cn an ui m ο oom 蠭 CO cn private o CO CO ο o should ifiVI j〇R ►— · ro s 筇 ο oom Ping I— * • ^ 3 5) m Ο oo fun a bismuth CO ο CJ CO o CO smmmm N &gt; o 00 o ro S m骝 骝 ΓΟ o rss private ro Ui 翊 o 〇 黩 [φ CO CJ1 CJ1 m male mo &lt; jy o 〇 »ro mm girl I— · s» — · cn oso 翼 杂 m σ &gt; s CJ1 CJ! 9) cn moo 嬲 -43- (know first read the notes on the back and then write this page) I set. Ordered · This paper size is applicable to China National Standard (CNS) A 4 specifications (210 X 297 mm) 81.9.20,000 203556

V. Description of the invention (AlJ A6 B6 Central Standards of the Ministry of Economic Affairs / ζ, printed by the Cooperative Society of the Industrial and Commercial Development Bureau, VBfr Vnfr vifr Φκ IU 11 1 minute and m 3 CO CD Ο CO I— · Ο CO He as ο α &gt; rsj ο s Wings cn m Ξ smmn | rv &gt; 00 ο fs &gt; Cd Ο CO o erqu Μ rsj cn ο Ν &gt; CO sm male 09 CO ΡΟ α &gt; ο CO 1— * he ο o 隳 1200 1000 so on ijt z * · ^, R thin o bazheng α s CO cn R 掸 ο o 9tk 'w. 1— * CO F 蠹 众 ο ο o mm mm ►- · 1— * • o ΟΙ ο cn ΓΟ cd rs &gt; ο is &gt; oi 蘧 ο ο omm 1— * ι— * 1— »灞 οι ο CJft 雄 -44- (please read the notes on the back before writing this page).

Line I table paper is again applicable to the Chinese National Standard (CNS) A 4 specifications (210 X 297 mm) 81.9.20,000 65 6 a, η η

A V. Description of the invention (fight $) Printed by the Ministry of Economic Affairs Central Standards Bureau Employee Consumer Cooperative to tear Vtfr foot Erbium UI 11 l points and points m 1 1 ο h- · SR 1 on Ο sleepy 〇π Ο 1 1 CJl cn 隳邸 邙 私 On CJ1 CJ1 〇cn m 蓰 + 〇 »— · ο m ^ o οι Spicy + 1 1 1 1 II II Yue Jue 1 1 I private 1— · CO 09 Private cn 〇o ο Yue iCk 1— · private 00 ta lien CJ1 o ut 1— * ►- * 9 — »cn o cn 嬲 m 4 〇» — »ο cn o cn -45- (please read the precautions on the back first and then fill in this page). Pack-order.

丨 Line I This paper is again applicable to the Chinese National Standard (CNS) A 4 specifications (210 X 297 mm) 81.9.20,000 A6 B6 203556 V. Description of the invention (U_ +) (# 先 Ｍ 读 Remarks on the back of the copy Page 2 Example 2 In this example, using large squeezed meal, 600ml of water was added to 200g of the soybean squeezed meal, then, take a 100s animal stub to add 200ml of water to it, and then use glass The homogenizer crushed it, and centrifuged the resulting tissue homogenate at 1 500rpm, took the clear liquid 100m1 and added it to the above-mentioned squeezed meal, and used it as a buffer for sodium sulfonate. 0。 Add acid to it, adjust its pH to 10. 0. Then, keep it at 40t: place. Thin element reaction You use kletto photoelectric colorimeter (No. 540, use filter) to measure, after 8 hours, confirm that the reflex is finished. Then, 40 ml of 0.1 M sodium silicate and shed acid grade liquid (pH 10 · 0) were added to the reactant, and commercially available chymotrypsin was added to the extent of 0.1 μM, and then 4 Ot; place it next. When the decomposition reaction caused by fermentation was over, he confirmed that he would take out the anti-moles one by one at 0.2m 1 and then titrate the ammonia in the anti-anhydrogen by Conway titration. After 10 hours, the occurrence of ammonia ceased, and the anti-plaque ended. Finally, the sowed reaction powder is filtered and washed with water to obtain the fermentation product of the main ingredient of the hair nourishing liquid. Example 3 of the Rigong Consumer Cooperative Society of the Ministry of Economic Affairs of the Ministry of Economic Affairs of India In this example, the squeezed meal of red beans is used as a matrix Then, take the fat products obtained from FIB micellium, float in 100ml of distilled water, and then use ka -4 6 _ 81.9.20,000. This paper is again applicable to the Chinese 8 standard (CNS) A 4 specifications (210 X 297 mm)% V. Description of invention Ur) A6 B6 Printed by pp — M a of the Beigong Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economy pp — M a is placed on top of 2 to clarify the B rit acid, acetic acid, and value 9 Titrate its vibration level to 8. Secondly, hydrogen is introduced into the enzyme for precipitation, while Protein "uses Robinson's buffer and extracts the resulting in 40 C, and then converts the obtained inverse 4 to 0 m 1 of water C. Then, the resulting solution 5 buffer (0 r ch mixer was stirred and mixed for 2 minutes, wrapped in the chamber for 0 minutes, and then centrifuged at 2000 rpm, sowing it, adding the supernatant to the above red bean press meal , And then ton-Robinson grade balance solution (dish acid, vine caustic soda bar) was added as a buffer solution, and its pH was .0. Then, the resulting mixture was kept at 3510 hours. The reaction proceeded like With 0.05M enzyme "acid, the commercially available papain made by ganluolysis in water, alkalized for a few hours, and then add yeast to it, so that papaya protein and then add water to the refined papain (Each g papaya water 10〇1〇1), and then add 81 ^ Pao 〇11- flushing liquid in it • The pH value is adjusted to 9.0, the mixture is added to the above reaction solution. Then. Keep it. After the reaction is over, take the reaction solution successively and take 0.2m 1 for fermentation titration to confirm. After 12 hours, the deflation is over. Finally, the material is filtered to obtain the filtration of the main component of the meal hair liquid. In the examples, the city is used Tofu residue sold as a base, add 50 to 100g of tofu residue, and guarantee At 50, the commercially available D. aureus product was dissolved in distilled water with 1% m 1 and added to the tofu residue. 5m 1 of phosphoric acid (6M, PH 7.0) was added to it and added at 40 (Please read the notes on the back and then write this page) This paper is also suitable for the aa family standard (CNS> A 4 specifications &lt; 210 X 297 mm> 81.9,20,000 ^ 03556 A6 B6_ V. Invention Description (Bucket b) Keep it under υ for 10 hours, during which time it is stirred at all times. There is a confirmation of the end of the reaction, and all use of the Valubruck pressure gauge to stop the state of the two gasification, to confirm the reaction of the enzyme End. Secondly, add 10 g of commercially available gastroenzyme (Cudahy, 1 / 10,000 USP soluble prionase) in a state of being dissolved in 20% ethylenic acid 1 1 and add it to the above-mentioned final reaction mixture. Then Then, the ammonium fi acid was used to adjust its ϊ &gt; H to 7.5. The resulting mixture was placed at 35υ for 20 hours. Then, 5 g of diatomaceous earth filter rib was added to it, and then filtered. The pH is 7.0. Place it under 3710. Regarding the confirmation of the anti-plaque, the reaction was taken out successively with a child of 0.5m 1 and the Placed in the outside of the Kangshu device, mix the reactant with 1ml of potassium silicate solution pre-installed in the device. • Determine the amount of change in the volatile atmosphere by titration. After 10 min, the reaction is over. Finally In a general method, the resulting anti-molecular material is filtered to obtain a filtrate of the main ingredients of the hair-raising liquid. The content of each component in the filtrate of every 100s of the meal-hair liquid broadcasted in Examples 2, 3, and 4 shown in Table 17. Expressed in mg. ------------------ 丄 ---- 装 ------ # ------ 线 {· (Please read first (Notes on the back of the Qiong will be written on this page.) This is printed by the Employee Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs-4 8-This paper scale applies to the Chinese Western Standard (CNS) Grade 4 (210 X 297 mm) 81.9.20,000 203556 A6 B6 V. Description of invention (η) The Central Bureau of Standards of the Ministry of Economic Affairs I®: printed by the industrial and consumer cooperatives JH r〇mmmm Luan m · ro Ol ο u \ ssomm private s fv &gt; Suspect B m · m ο o § CO ο ο -J s 2000 lysine 丨 twVI w S o er oo fei cai CJ1 ο CO U1 o CO U1 om 雄 1— · a sister rs &gt; ο gos CD wing_ should h- * 胡 u! 岩rs &gt; o thin oo too S s Yaner ο oom ί— * ο do o 〇ioo Yixiong C0 ο CO oo 1500 valeric acid road S 〇1 o -4 〇 尔 鬵 C9 Ο 1500 2500 arginine-49 -This paper is again applicable to the National Standards (CNS) A 4 specifications (<210 X 297 mm) of the country t 81.9.20,000 ------------------ people ---- Installed ------. 玎 ------ ί, (please read the notes on the back of the table before writing this page) ^ 03556 A6 B6 Fifth, the invention description (U) the consumption of the Central Standard General Staff of the Ministry of Economic Affairs Printed by cooperatives Example 3 Example 2 ΓΟ α »〇3000 5000 Interest: 3 m cn ο ο S ο 1500 Alanine Juno ο 1500 Glutamate CO ο ο 1000 _ 1300 Glycine rs &gt; α &gt; ο ο 1200 proline i CO § priv ο ΟΙ ο serine 3000 15000 20000 vitamin g («g)! 1 _] ΟΙ ο ο CO ο ο cn ο copper (/ U g) 60.0 60.0 65.0 曛 昤 类 i ! _1 CD Cn ο h- »Ο Η-» ma ►- * ι— * ΓΟ Ο 10.0 Palmitic acid CJi Ο CO Ο ro ο Stearic acid-50- This paper is again applicable to the Chinese National Standard (CNS) A4 Specifications (210 X 297 mm) 81.9.20,000 ----- ------------- 一 ----- 装 ------. 玎 ------ Articles (please read the notes on the back before writing this page) A6 ⑼ Bismuth Station-Printed by the Employees ’Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs-V. Description of the invention (1 ^) Example 4 Example 3 Example 2 Erbium 1 1 1 1 1 1 1 1 1 1 Alginic acid 1 1 1 1 1 I 1 1 1 Sanpanic acid 1 1 1 ο Η- »1 1 1 Twenty tetrakanoic acid 1— * 〇1 1 1 Η-» ο Spicy + stimuli》 〇 »— * I 1 1 ο Ten t sulfenoic acid ►— * cr &gt; 〇Η- »ΓΌ Ο» — * CO ο m 鼸 ΓΟ ατ ο Η- »private ο οι ο linoleic acid 1— · I— * Ο 00 ο CO ο linolenic acid 1— * ο 1 1 1 h- »ο 無 ο h- * ο 1— * ο—20-dienoic acid Ο Η- * 1 Lu» 1 I 1 Si ΓΓ * m hf s 1 1 i ο 1— * \ 1 1 20 Enoic acid-5 1- ------------------ 1 ----- install -------- # ------ Μ (please read the back first The matters to be remembered will be written on this page.) This paper scale is applicable to the Chinese Gardener Standard (CNS> A4 specifications (210 X 297 mm) 81.9.20,000 0¾¾¾6 5. V. Description of the invention ([0) Composition dioctanoic acid grams Ruba Norton acid twenty-two hexaenoic acid Example 2-0.1 0. 1 Example 3 1 — 0.1 Example 4 — 0.1 — (#Read the note on the back of the page ± items before writing this page) • Binding. Order-The effect of the C invention printed by the Beigong Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs] Compared with the conventional hair-raising liquid, the method of preparing hair-raising liquid described in Shen Fan-li's painting has much better hair removal prevention effects, hair-growing and hair-raising effects, which is significantly more than expected.啻 毛 效应. In addition, in addition to amino acids and fatty acids, the resulting fermentation product contains vitamin E, copper, _ 椤 and other types of ingredients, but some moldy ingredients and amino acids and fatty acids Multiplying effect, the badger can get hair raising liquid with more improved hair raising effect. -52 ^ The size and degree of the paper are in accordance with the specifications of the Chinese standard (CNS) A4 (210 X 297 public goods) 81.9.20,000

Claims (1)

Amendment 82.2. 16 [Supplement 4. * 月 月 A7 Β7 C7 D7 6. Application for patent scope 1. A method of making hair-raising liquid, the special image of which is to extract rice bran containing rice bran, soybeans, red bean press, Tofu Tong and other vegetable proteins and an aqueous base of vegetable fats and fats, to which chymotrypsin, papaya protein syrup, prionase, etc. are added at a ratio of 1: 1 to 1000, which can decompose the above vegetable proteins and plants Enzymes of sex lipids, or after adding 麴 豳 in a ratio of 1: 100, add a buffer solution to make the pH of the above-mentioned aqueous substrate to be 6 ~ 1 1, and then place the above-mentioned aqueous substrate at a temperature of 3 5 ~ 45P for 5 ~ It takes 10 hours to ferment and make the main ingredient of the hair liquid. (Please read the precautions on the back before writing this page) 丨 Installed. Ordered. Spring · The Ministry of Economic Affairs, Zhonghuan quasi-bureau Λ industry consumer cooperatives printed this paper ίΜΛϋlϋ + ΒPostage (cns) A4 specifications (210Χ 297 tournament