TW202523676A - 陽離子交換層析期間之高鹽洗滌以去除產品相關的雜質 - Google Patents

陽離子交換層析期間之高鹽洗滌以去除產品相關的雜質 Download PDF

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Publication number
TW202523676A
TW202523676A TW114107974A TW114107974A TW202523676A TW 202523676 A TW202523676 A TW 202523676A TW 114107974 A TW114107974 A TW 114107974A TW 114107974 A TW114107974 A TW 114107974A TW 202523676 A TW202523676 A TW 202523676A
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TW
Taiwan
Prior art keywords
sodium chloride
acetate
wash buffer
cation exchange
wash
Prior art date
Application number
TW114107974A
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English (en)
Chinese (zh)
Inventor
馬爾哈爾 Ｒ 安布哈卡
路易斯 狄雅茲
娜塔莉 歌梅茲
班傑明 Ｊ 提洛斯頓
Original Assignee
美商安進公司
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Publication date
Application filed by 美商安進公司 filed Critical 美商安進公司
Publication of TW202523676A publication Critical patent/TW202523676A/zh

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/145—Ultrafiltration
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2315/00—Details relating to the membrane module operation
    • B01D2315/16—Diafiltration
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Water Supply & Treatment (AREA)
  • Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
TW114107974A 2019-11-07 2020-11-06 陽離子交換層析期間之高鹽洗滌以去除產品相關的雜質 TW202523676A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962931874P 2019-11-07 2019-11-07
US62/931,874 2019-11-07

Publications (1)

Publication Number Publication Date
TW202523676A true TW202523676A (zh) 2025-06-16

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
TW114107974A TW202523676A (zh) 2019-11-07 2020-11-06 陽離子交換層析期間之高鹽洗滌以去除產品相關的雜質
TW109138883A TWI878377B (zh) 2019-11-07 2020-11-06 陽離子交換層析期間之高鹽洗滌以去除產品相關的雜質

Family Applications After (1)

Application Number Title Priority Date Filing Date
TW109138883A TWI878377B (zh) 2019-11-07 2020-11-06 陽離子交換層析期間之高鹽洗滌以去除產品相關的雜質

Country Status (14)

Country Link
US (1) US20220372071A1 (enExample)
EP (1) EP4055042A1 (enExample)
JP (2) JP2022554283A (enExample)
KR (1) KR20220092958A (enExample)
CN (1) CN114651007A (enExample)
AR (1) AR120398A1 (enExample)
AU (1) AU2020377919A1 (enExample)
BR (1) BR112022008782A2 (enExample)
CA (1) CA3160208A1 (enExample)
CL (2) CL2022001169A1 (enExample)
IL (1) IL292602A (enExample)
MX (1) MX2022005594A (enExample)
TW (2) TW202523676A (enExample)
WO (1) WO2021091933A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2024500747A (ja) * 2020-12-18 2024-01-10 キニクサ ファーマシューティカルズ， リミテッド タンパク質組成物ならびにそれを産生及び使用するための方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU9034298A (en) * 1997-08-25 1999-03-16 Walter H. Runkis A simple, environmentally benign, method for purifying protein
CN105315323A (zh) * 2007-10-30 2016-02-10 健泰科生物技术公司 通过阳离子交换层析进行的抗体纯化
WO2010127069A1 (en) * 2009-04-29 2010-11-04 Schering Corporation Antibody purification
US20120238730A1 (en) * 2011-03-15 2012-09-20 Abbott Laboratories Integrated approach to the isolation and purification of antibodies
CN104628846B (zh) * 2013-11-06 2019-12-06 三生国健药业（上海）股份有限公司 重组蛋白质的纯化方法
EP3145951A1 (en) * 2014-06-24 2017-03-29 InSight Biopharmaceuticals Ltd. Methods of purifying antibodies
RS65573B1 (sr) * 2014-08-04 2024-06-28 Hoffmann La Roche Bispecifični antigen vezujući molekuli koji aktiviraju t ćelije
CA2963692A1 (en) * 2014-10-09 2016-04-14 Engmab Ag Bispecific antibodies against cd3epsilon and ror1
JP6699085B2 (ja) * 2015-03-25 2020-05-27 東ソー株式会社 Ｆｃ結合性タンパク質の精製方法
TWI798179B (zh) * 2016-06-17 2023-04-11 美商建南德克公司 多特異性抗體之純化
WO2018237159A1 (en) * 2017-06-21 2018-12-27 Cephalon, Inc. Cation exchange chromatography wash buffer
CN110305217B (zh) * 2018-03-27 2022-03-29 广州爱思迈生物医药科技有限公司 双特异性抗体及其应用

Also Published As

Publication number Publication date
AU2020377919A1 (en) 2022-06-16
TW202132323A (zh) 2021-09-01
MX2022005594A (es) 2022-06-09
EP4055042A1 (en) 2022-09-14
US20220372071A1 (en) 2022-11-24
CN114651007A (zh) 2022-06-21
CL2022001169A1 (es) 2023-01-27
KR20220092958A (ko) 2022-07-04
CL2024001792A1 (es) 2024-11-08
TWI878377B (zh) 2025-04-01
IL292602A (en) 2022-07-01
AR120398A1 (es) 2022-02-09
WO2021091933A1 (en) 2021-05-14
JP2026048656A (ja) 2026-03-17
BR112022008782A2 (pt) 2022-07-26
JP2022554283A (ja) 2022-12-28
CA3160208A1 (en) 2021-05-14

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