TW202338076A - Beer-flavored beverage - Google Patents

Abstract

A beer-flavored beverage having a proline content of 15-75 mg/100 mL, a total polyphenol amount of 60-200 mass ppm, a pyroglutamic acid content of 50-300 mg/L, and an exterior fermentation degree of 70.0-95.0%.

Description

Beer flavored drinks

The present invention relates to beer-flavored beverages.

In the past, we have researched and provided various beer-flavored beverages in response to the recent diversified preferences of consumers. For example, Patent Document 1 describes a method for producing a fermented malt beverage, which aims to provide a fermented malt beverage that uses malt as a raw material and has a good balance of aroma and refreshing feeling. The step of feeding a mixture of fermentation raw materials and water with a ratio of 50 to 66 mass % after saccharification, boiling and preparing a fermentation raw material liquid, and inoculating yeast into the fermentation raw material liquid obtained by the aforementioned feeding step to perform fermentation. Steps to produce a fermented malt beverage with a bitterness value of 10~30BU. [Prior technical literature] [Patent Document]

[Patent Document 1] Japanese Patent Application Publication No. 2017-118824

[Problem to be solved by the invention]

Such fermented malt drinks may produce a feeling of fullness even in small amounts. Therefore, a beer-flavored beverage that makes it difficult to feel full that is unsuitable for beer-flavored beverages has been pursued. [Means to solve the problem]

The present invention provides a beer-flavored beverage whose proline content, total polyphenol content, pyroglutamic acid content, and apparent degree of fermentation are adjusted to a specified range. That is, the invention includes the following aspects. [1] A beer-flavored beverage characterized by a proline content of 15 to 75 mg/100 mL, a total polyphenol content of 60 to 200 mass ppm, a pyroglutamic acid content of 50 to 300 mg/L, and an apparent degree of fermentation of 70.0 to 95.0%. [2] For the beer-flavored beverage described in [1], the ratio of the total polyphenol content (X) (unit: mass ppm) to the proline content (Y) (unit: mg/100mL) [(X)/(Y )] is 1.5~5.5. [3] For the beer-flavored beverage described in [1] or [2], the original extract concentration is 6.0~20.0% by mass. [4] A beer-flavored beverage as described in any one of [1] to [3], wherein the content of pyroglutamic acid (Z) (unit: mg/L) and the content of proline (Y) (unit: mg/100mL ) ratio [(Z)/(Y)] is 1.0~7.5. [5] The beer-flavored beverage as described in any one of [1] to [4], wherein the aforementioned beer-flavored beverage is a fermented beer-flavored beverage. [6] For example, the beer-flavored beverage described in any one of [1]~[5] has a color of 5.0~20.0EBC. [7] For example, the pH of the beer-flavored beverage described in any one of [1] to [6] is 3.0 to 5.0. [8] For example, the beer-flavored beverage described in any one of [1] to [7] has a lactic acid content of 40 to 950 ppm by mass. [9] For example, the beer-flavored beverage described in any one of [1] to [8] has a phosphoric acid content of 40 to 800 ppm by mass. [10] The beer-flavored beverage described in any one of [1] to [9] contains ingredients derived from hops. [11] A method for manufacturing a beer-flavored beverage, which is characterized by a total polyphenol content of 60 to 200 ppm by mass, a proline content of 15 to 75 mg/100 mL, and a pyroglutamic acid content of 50~300mg/L, and the apparent fermentation degree is set as 70.0~95.0%. [12] A method for improving the aroma of beer-flavored beverages, characterized by the beer having a total polyphenol content of 60 to 200 ppm by mass, a proline content of 15 to 75 mg/100 mL, and a pyroglutamic acid content of 50 to 300 mg/L. In flavored beverages, the apparent fermentation degree is adjusted so that the apparent fermentation degree becomes 70.0~95.0%. [Effects of the invention]

According to a more suitable aspect of the present invention, a beer-flavored beverage with a satiety that is difficult to feel uncomfortable is provided. Furthermore, according to a more suitable aspect of the present invention, a beverage that can become more like a beer-flavored beverage is provided, for example, a beer-flavored beverage that has a rich taste derived from wheat while suppressing bland taste.

Regarding the numerical range described in this specification, the upper limit value and the lower limit value can be combined arbitrarily. For example, when the numerical range is described as "preferably 3.0 to 15, more preferably 3.2 to 13", the range of "3.0 to 13" or the range of "3.2 to 15" is also included in the numerical range described in this specification. Also, for example, when the numerical range is described as "preferably 30 or more, more preferably 40 or more, and preferably 100 or less, more preferably 80 or less", the range of "30~80" or "40~ The range of "100" is also included in the numerical range described in this manual. In addition, as a numerical range described in this specification, for example, the description of "60 to 100" means the range of "60 or more and 100 or less". Furthermore, among the regulations of the upper limit value and the lower limit value described in this specification, you can appropriately select from the respective options and arbitrarily combine them to define the numerical range from the lower limit value to the upper limit value. In addition, various requirements described as preferred aspects described in this specification may be combined in plural.

1. Beer-flavored beverages In this specification, the term "beer-flavored beverages" refers to carbonated beverages containing alcohol that have a beer-like flavor. That is, the beer-flavored beverages in this specification also include any carbonated beverages with beer flavor unless otherwise specified. Therefore, "beer-flavored beverages" include not only malt-fermented beverages that use yeast to ferment malt, hops and water as raw materials, namely beer, or fermented beer-flavored beverages, but also include esters or Carbonated drinks with added beer flavorings such as higher alcohols or lactones. Examples of beer flavors include isoamyl acetate, ethyl acetate, n-propanol, isobutanol, acetaldehyde, ethyl caproate, ethyl caprylate, isopentyl propionate, linalol, and geraniol. , citral, 4-vinylguaiacol (4-VG), 4-methyl-3-pentenoic acid, 2-methyl-2-pentenoic acid, 1,4-cineole, 1,8 -Cineole, 2,3-diethyl-5-methylpyridine , γ-decanolide, γ-undecylactone, ethyl caproate, 2-methylethyl butyrate, n-ethyl butyrate, myrcene, citral, limonene, maltol, ethyl malt Alcohol, phenylacetic acid, furanone, furfural, methylthiopropionaldehyde, 3-methyl-2-butene-1-thiol, 3-methyl-2-butanethiol, diacetyl, ferulic acid , geranic acid, geranyl acetate, ethyl butyrate, caprylic acid, capric acid, 9-decenoic acid, nonanoic acid, myristate, propanoic acid, 2-methylpropanoic acid, γ-butyrolactone, 2-amine Acetophenone, ethyl 3-phenylpropionate, 2-ethyl-4-hydroxy-5-methyl-3(2H)-furanone, dimethylsulfone, 3-methylcyclopentane-1 ,2-diketone, 2-methylbutanal, 3-methylbutanal, 2-methyltetrahydrofuran-3-one, 2-acetylfuran, 2-methyltetrahydrofuran-3-one, hexanal, Hexanol, cis-3-hexenal, 1-octen-3-ol, β-cineol, 4-mercapto-4-methylpentan-2-one, β-caryophyllene, β-lauric oil Alkene, furfuryl alcohol, 2-ethylpyra , 2,3-dimethylpyridine , 2-methylbutyl acetate, isopentyl alcohol, 5-hydroxymethylfurfural, phenyl acetaldehyde, 1-phenyl-3-buten-1-one, trans-2-hexenal, nonanal , phenylethyl alcohol. Furthermore, the beer-flavored beverage according to one aspect of the present invention may be an ale-flavored beverage brewed through a fermentation step using upper-fermenting yeast (saccharomyces, etc.), or may be an ale-flavored beverage brewed using lower-fermenting yeast (saccharomyces, etc.) Lager beer-flavored beverages, Pearson beer-flavored beverages, etc. brewed through the fermentation steps. In addition, the "fermentation" mentioned in this specification may be alcoholic fermentation that produces alcohol, or non-alcoholic fermentation that does not produce alcohol.

Although the alcohol content (ethanol content) of beer-flavored beverages is not particularly limited, it is preferably 0.5 (v/v)% or more, 1.0 (v/v)% or more, and more preferably 2.0 (v/v)% or more. , more preferably 3.0 (v/v)% or more, still more preferably 3.5 (v/v)% or more, particularly preferably 4.0 (v/v)% or more, and more preferably 10.0 (v/v)% or less, more preferably 9.0 (v/v)% or less, still more preferably 8.0 (v/v)% or less, even more preferably 7.0 (v/v)% or less, particularly preferably 6.5 (v/v)% or less , it can also be set below 6.0(v/v)%. In addition, in this manual, the alcohol content is expressed as a volume/volume basis percentage ((v/v)%). In addition, the alcohol content of the drink can be measured by any known method. For example, it can be measured by a vibration density meter. The alcohol content can be adjusted by appropriately setting the addition of diluting water or carbonated water, the type of raw materials (malt, ground corn, sugar liquid, etc.), the amount of raw materials, the type of enzyme, the amount of enzyme added, and the time of adding enzyme points, saccharification time in the feed tank, protein decomposition time in the feed tank, pH in the feed tank, pH in the feeding step (wort production step before adding malt to yeast), and pH adjustment time The amount of acid to be added, the time point for pH adjustment (when feeding, during fermentation, at the end of fermentation, before beer filtration, after beer filtration, etc.), and the set temperature of each temperature zone when preparing wort (including saccharification) And the residence time, the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, yeast species, yeast addition amount, yeast proliferation number, yeast removal time point, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.), the addition of spirits or brewing alcohol, etc.

The beer-flavored beverage of the present invention has an apparent fermentation degree of 70.0 to 95.0%. Beer-flavored beverages with a high degree of fermentation in appearance can suppress the feeling of fullness, but the umami taste is reduced and becomes a bland taste, which tends to reduce the umami taste of wheat. Therefore, in the beer-flavored beverage of the present invention, the saccharification conditions and fermentation conditions are adjusted so that the apparent fermentation degree becomes 70.0~95.0%, so that the proline content is 15~75 mg/100mL and the pyroglutamic acid content is 50~ 300mg/L, and the total polyphenol content is 60~200 ppm by mass. The present invention, by adjusting the apparent degree of fermentation, proline content, pyroglutamic acid content and total polyphenol content, can provide a feeling of fullness without causing discomfort, and can drink a lot of beer-flavored beverages, which is a further better aspect. The invention can provide a beer-flavored beverage that can feel the umami taste of wheat, or a beer-flavored beverage with a rich taste derived from wheat.

Although the apparent fermentation degree of the beer-flavored beverage of the present invention is 70.0% or more, from the viewpoint of becoming a beer-flavored beverage that suppresses an uncomfortable feeling of fullness, it is preferably 71.0% or more, more preferably 72.0% or more, and more preferably The best is more than 73.0%, the best is more than 74.0%, the particularly good is more than 75.0%, and it can also be more than 76.0%, more than 78.0%, more than 80.0%, more than 81.0%, more than 82.0%, more than 83.0%, Above 84.0%, above 85.0%, or above 86.0%. Although the apparent fermentation degree of the beer-flavored beverage of the present invention is 95.0% or less, from the viewpoint of becoming a beer-flavored beverage that further enhances the rich taste derived from wheat, it is preferably 94.5% or less, and more preferably 94.0% or less. , more preferably 93.5% or less, still more preferably 93.0% or less, particularly preferably 92.0% or less, and may also be 91.0% or less, or 90.0% or less.

In this specification, the so-called "appearance of fermentation" refers to the proportion of sugar concentration that can be consumed by yeast as a nutrient source for alcohol fermentation among the total sugar concentration contained in the liquid before fermentation. For example, the apparent fermentation degree AA of the beer-flavored beverage of the present invention can be calculated from the following formula (1). Formula (1): AA(%)=100×(P-Es)/P In the above formula (1), "P" is the original extract (original wort extract), which can be determined by the "BCOJ beer analysis method ( It is measured according to the method described in "Compiled by Japan Brewing Association Fermentation and Beer Brewing Association, revised edition on November 1, 2004". In addition, "Es" represents the appearance extract of the beer-flavored beverage. The appearance extract can be calculated by the following formula (2), for example, as described in "BCOJ Beer Analysis Method (Compiled by Japan Brewing Association Fermentation and Beer Brewing Association, revised edition on November 1, 2004)". Formula (2): Es=-460.234+662.649×D-202.414×D ² (in formula (2), D is the specific gravity of degassed beer-flavored beverage). In addition, the apparent extract "Es" may have a negative value based on D in the above formula (2), so the calculated apparent fermentation degree may exceed 100%.

In addition, the degree of fermentation of the appearance of beer-flavored beverages can be adjusted by appropriately setting the addition of diluting water or carbonated water, the type of raw materials (malt, ground corn, sugar liquid, etc.), the amount of raw materials, the type of enzyme, and the enzyme (also Including glycolytic enzyme, isomerizing enzyme, etc.), the temperature during the enzyme reaction, the time point when the enzyme is added, the saccharification time, the pH during saccharification, the temperature during saccharification, and the feeding step (malt is added to The pH in the wort manufacturing step before adding yeast), the temperature in the feeding step, the time of wort filtration, the set temperature and residence time of each temperature zone when preparing wort (including saccharification), and the pre-fermentation liquid Original extract concentration, original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, yeast species, yeast addition amount, yeast proliferation number, yeast removal time point, fermentation temperature, fermentation time, pressure setting, Carbon dioxide concentration, etc.), cooling time point, cooling temperature, cooling time, etc.

The original extract (O-Ex) concentration (original wort concentration) in the beer-flavored beverage of the present invention is 6.0 to 20.0 mass%. If the concentration of the original extract in beer-flavored beverages is increased, it will be difficult to perceive the bland taste. Therefore, the original extract concentration of the beer-flavored beverage according to one aspect of the present invention is preferably 6.5 mass% or more, more preferably 7.0 mass% or more, further preferably 7.5 mass% or more, and still more preferably 7.8 mass% or more. , the best value is 8.0 mass% or more, but it can also be 8.2 mass% or more, 8.5 mass% or more, 8.6 mass% or more, 8.7 mass% or more, 8.8 mass% or more, 8.9 mass% or more, 9.0 mass% or more, 9.1 mass% % or more, 9.2 mass% or more, 9.3 mass% or more, 9.4 mass% or more, 9.5 mass% or more, 9.6 mass% or more, 9.7 mass% or more, 9.8 mass% or more, 9.9 mass% or more, 10.0 mass% or more, 10.1 mass % or more, 10.2 mass% or more, 10.3 mass% or more, 10.4 mass% or more, 10.5 mass% or more, 10.6 mass% or more, 10.7 mass% or more, 10.8 mass% or more, 10.9 mass% or more, or 11.0 mass% or more. In addition, the original extract concentration of the beer-flavored beverage according to one aspect of the present invention is preferably 18.0 mass% or less, and more preferably 17.0 mass% or less, from the viewpoint of becoming a beer-flavored beverage that suppresses an uncomfortable feeling of fullness. , more preferably 16.0% by mass or less, still more preferably 15.0% by mass or less, particularly preferably 14.0% by mass or less, but it may be 13.8% by mass or less, 13.6% by mass or less, 13.4% by mass or less, or 13.2% by mass or less. 13.0 mass% or less, 12.8 mass% or less, 12.7 mass% or less, 12.6 mass% or less, 12.5 mass% or less, 12.4 mass% or less, 12.3 mass% or less, 12.2 mass% or less, 12.1 mass% or less, 12.0 mass% or less. The concentration of the original extract can be adjusted by appropriately setting the addition of dilution water or carbonated water, the type of raw materials (malt, ground corn, sugar solution, etc.), the amount of raw materials, the time of wort filtration, the pH of wort filtration, It is carried out according to the boiling time, boiling temperature, the amount of spirits added, the amount of brewing alcohol added, etc. The original extract (original wort extract) of the beer-flavored beverage of the present invention can be obtained, for example, by modifying the BCOJ Beer Analysis Method (published by the Japan Brewing Association, compiled by the International Technical Committee [Analysis Committee] of the Beer Brewing Association in 2013 Supplement and revise the recorded method for measurement.

The proline content of the beer-flavored beverage according to one aspect of the present invention makes it a beer-flavored beverage that suppresses discomfort and a weak taste, and at the same time, it becomes a beer that further enhances the rich taste, drinking feeling, and richness of the taste derived from wheat. From the perspective of flavored beverages, it is 15 mg/100mL or more, preferably 16 mg/100mL or more, more preferably 17 mg/100mL or more, further preferably 18 mg/100mL or more, still more preferably 19 mg/100mL or more, and particularly preferably 20 mg. /100mL or more, and may also be 21mg/100mL or more, 23mg/100mL or more, or 25mg/100mL or more. On the other hand, if the beer-flavored beverage contains a large amount of proline, the feeling of fullness will easily occur. Therefore, the proline content of the beer-flavored beverage is 75 mg/100mL or less, preferably 73 mg/100mL or less, and more preferably It is 71 mg/100mL or less, more preferably 69 mg/100mL or less, still more preferably 67 mg/100mL or less, particularly preferably 66 mg/100mL or less, and it may also be 65 mg/100mL or less, 63 mg/100mL or less, or 61 mg/100mL or less. , or below 60mg/100mL. In this specification, the proline content can be measured, for example, using an amino acid automatic analyzer model L-8800A manufactured by Hitachi, Ltd., etc.

Proline is an amino acid contained in nitrogen-containing raw materials such as malt. Since it is not used by yeast as a nutrient source during the fermentation step, it does not have the above-mentioned impact on the flavor of the final beer-flavored beverage. Proline may be contained in the raw materials of the beer-flavored beverage, or may be added separately in the production step (for example, purified proline). The content of proline can be adjusted by appropriately setting the addition of diluting water or carbonated water, and amino acid-containing raw materials (malt, wheat, cracked corn, sugar solution, yeast extract, soybeans, peas, purified proline etc.), the amount of raw materials, the type of enzyme (protease, etc.), the amount of enzyme added, the time of adding enzyme, the saccharification time in the feed tank, the protein decomposition time in the feed tank, the feed pH in the tank, pH in the feeding step (the wort manufacturing step before adding malt to yeast), the amount of acid added when adjusting the pH, and the time point of pH adjustment (when feeding, during fermentation, during fermentation) The set temperature and residence time of each temperature zone at the end, before beer filtration, after beer filtration, etc.), when preparing wort (including saccharification), the original extract concentration of the pre-fermentation liquid, and the original extract concentration in the fermentation step , Fermentation conditions (oxygen concentration, ventilation conditions, yeast species, yeast addition amount, yeast proliferation number, yeast removal time, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.), spirits or brewing alcohol Wait for the addition to proceed.

The total polyphenol content of the beer-flavored beverage according to one aspect of the present invention can suppress the unpleasant taste of the beer-flavored beverage and at the same time, it can further enhance the rich taste, drinking feeling, and richness of the taste suitable for the beer-flavored beverage. From the perspective of beer-flavored beverages, it is 60 mass ppm or more, preferably 65 mass ppm or more, more preferably 70 mass ppm or more, further preferably 71 mass ppm or more, more preferably 72 mass ppm or more, and still more preferably 73 ppm by mass or more, and may be 75 ppm by mass or more, 80 ppm by mass or more, 85 ppm by mass or more, 90 ppm by mass or more, 95 ppm by mass or more, or 100 ppm by mass or more. On the other hand, if the amount of total polyphenols is high, the taste will become stronger and an uncomfortable feeling of fullness will easily occur. Therefore, the total polyphenol content of the beverage of the present invention is 200 mass ppm or less, preferably 195 mass ppm or less, more preferably 190 mass ppm or less, still more preferably 180 mass ppm or less, still more preferably 170 mass ppm or less. , and may be 160 ppm by mass or less, 150 ppm by mass or less, 140 ppm by mass or less, 130 ppm by mass or less, or 120 ppm by mass or less.

Polyphenols refer to compounds in which two or more hydrogens of aromatic hydrocarbons may be replaced by hydroxyl groups. Examples of polyphenols include flavonols, isoflavones, tannins, catechins, flavonoids, and anthocyanins. The so-called "total polyphenols amount" in the present invention refers to the total amount of these polyphenols contained in the beer-flavored beverage.

The amount of total polyphenols can be adjusted by appropriately setting the addition of diluting water or carbonated water, the type of raw materials (malt, ground corn, sugar solution, etc.), the amount of raw materials, the type of enzyme, the amount of enzyme added, and the amount of enzyme added. time point, polyphenol polymerization conditions in the feed tank (temperature, stirring speed, etc.), pH in the feed tank, pH in the feeding step (wort production step before adding malt to yeast), malt Juice filtration time, set temperature and residence time of each temperature zone when preparing wort (including saccharification), original extract concentration of pre-fermentation liquid, original extract concentration in fermentation step, fermentation conditions (oxygen concentration, aeration) Conditions, yeast species, yeast addition amount, yeast proliferation number, yeast removal time, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.), cooling time, cooling temperature, cooling time, beer filtration form (silica Algae, membranes, sheets, boxes, filters, etc.), activated carbon, stabilizers added during beer filtration (silica gel, PVPP (polyvinyl polypyrrolidinone), bentonite, tannin, bentonite, etc.) etc. Furthermore, the total polyphenol content of the beer-flavored beverage of the present invention can be controlled, for example, by adjusting the usage amount of raw materials with high polyphenol content, such as barley malt and malt husk (husk). Specifically, the total polyphenol content can be increased by increasing the usage amount of raw materials such as malt that have a high polyphenol content.

Generally speaking, husk (husk) malt, etc. has a high content of nitrogen and polyphenols, soybeans, yeast extract, peas, corn, corn processed products (cracked corn, corn gluten, etc.), wheat, wheat malt, etc. It contains more nitrogen but less polyphenols. Therefore, the proline content and total polyphenol content in beer-flavored beverages can be increased or decreased by adjusting the blending ratio of raw materials. The following are examples of representative methods (1) to (4) for increasing or decreasing the proline content and total polyphenol content by adjusting the blending ratio of raw materials. (1) By increasing the usage of hulled malt, etc., the proline content and total polyphenols content of beer-flavored beverages can be increased. (2) By increasing or decreasing the amount of soybeans, yeast extract, etc., the proline content of beer-flavored beverages can be increased or decreased while maintaining the total polyphenol content. (3) By increasing the usage of hulled malt, etc. and reducing the usage of soybeans, yeast extract, etc., while maintaining the proline content, the total polyphenol content is increased. (4) By reducing the amount of hulled malt, etc. and increasing the amount of soybeans, yeast extract, etc., the total polyphenol amount is reduced while maintaining the proline content.

The total polyphenol content of the beer-flavored beverage of the present invention can be determined, for example, by modifying the method described in the BCOJ Beer Analysis Method (issued by the Japan Brewing Association, edited and revised in 2013 by the International Technical Committee of the Beer Brewers Association [Analysis Committee]) to measure.

In the beer-flavored beverage of the present invention, from the viewpoint of further improving the taste of the beer-flavored beverage, it is preferable that at least part of the proline or polyphenols is derived from malt. Furthermore, in the beer-flavored beverage of the present invention, from the viewpoint of becoming a beverage that further enhances the astringent feeling of the beer-flavored beverage, at least part of the proline or polyphenols may be derived from soybeans, yeast extract, peas, and corn. , processed corn products (cracked corn, etc.), wheat, wheat malt, etc.

Furthermore, the beer-flavored beverage according to one aspect of the present invention may further contain grain-derived spirits (distilled liquor) as an alcohol component. In this specification, spirits refer to grains such as wheat, rice, buckwheat, corn, yam, sugar cane, etc. as raw materials, saccharified using malt or an enzyme as necessary, and fermented using yeast. Then, the wine is distilled. Cereals used as raw materials for spirits are preferably plants belonging to the family Gramineae, and more preferably wheat.

The content of pyroglutamic acid in the beer-flavored beverage according to one aspect of the present invention is 50 mg/L or more, preferably 52 mg/L or more, and more preferably 52 mg/L or more, from the perspective of imparting a better taste to the beer-flavored beverage. 54 mg/L or more, more preferably 56 mg/L or more, still more preferably 58 mg/L or more, particularly preferably 60 mg/L or more, and may also be 65 mg/L or more, 70 mg/L or more, or 75 mg/L or more, Above 80mg/L, above 85mg/L, or above 90mg/L. On the other hand, from the viewpoint of suppressing sourness unsuitable for beer-flavored beverages, the content of pyroglutamic acid is 300 mg/L or less, preferably 290 mg/L or less, more preferably 280 mg/L or less, and still more preferably 270 mg. /L or less, more preferably 260 mg/L or less, still more preferably 250 mg/L or less, and it may be 240 mg/L or less, 230 mg/L or less, 220 mg/L or less, 210 mg/L or less, or 200 mg/L. the following. Pyroglutamic acid may be contained in the raw materials of the beer-flavored beverage, or may be added separately in the production step (for example, purified pyroglutamic acid). The content of pyroglutamic acid can be adjusted by appropriately setting the addition of diluting water or carbonated water, the addition of purified pyroglutamic acid, and the raw materials containing pyroglutamic acid (malt, wheat, ground corn, sugar solution, yeast extract). materials, soybeans, peas, proline purified products, etc.), the amount of raw materials, the enzyme reaction time in the feeding step (raw materials such as malt are added to the wort production step before yeast is added), and the feeding step pH, the amount of acid added during pH adjustment, the time point of pH adjustment (when feeding, during fermentation, at the end of fermentation, before beer filtration, after beer filtration, etc.), when preparing wort (including saccharification) The set temperature and residence time of each temperature zone, the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, aeration conditions, yeast species, yeast addition amount, yeast proliferation number, yeast It is carried out by removing the time point, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.), adding spirits or brewing alcohol, etc. In addition, in this specification, the content of pyroglutamic acid can be measured, for example, by high-speed liquid chromatography.

The beer-flavored beverage according to one aspect of the present invention has a total polyphenol content (X) (unit: mass ppm) and a proline content (Y) (unit: mass ppm) from the viewpoint of not causing an uncomfortable feeling of fullness. mg/100mL) ratio [(X)/(Y)] is 1.5 to 5.5, but is preferably 1.6 or more, more preferably 1.7 or more, further preferably 1.8 or more, still more preferably 1.9 or more, and particularly preferably 2.0 The above may also be 2.2 or more, 2.4 or more, or 2.6 or more. On the other hand, from the perspective of making a rich-tasting beer-flavored beverage, the ratio of the total polyphenol content (X) (unit: mass ppm) to the proline content (Y) (unit: mg/100mL) [(X)/(Y)], preferably 5.4 or less, more preferably 5.3 or less, further preferably 5.2 or less, still more preferably 5.1 or less, particularly preferably 5.0 or less, and may also be 4.9 or less, 4.7 below, or below 4.5.

From the viewpoint of imparting a complex taste to the beer-flavored beverage according to one aspect of the present invention without causing an uncomfortable feeling of fullness, although the content (Z) of pyroglutamic acid (unit: mg/L) is the same as that of proline The ratio [(Z)/(Y)] of the content (Y) (unit: mg/100mL) is 1.0~7.5, but preferably 1.2 or more, more preferably 1.4 or more, further preferably 1.6 or more, still more preferably It is 1.8 or more, more preferably 2.0 or more, particularly preferably 2.5 or more, and it may be 2.6 or more, 2.7 or more, or 2.8 or more. On the other hand, from the perspective of making a beer-flavored beverage with a strong taste, the content of pyroglutamic acid (Z) (unit: mg/L) and the content of proline (Y) (unit: mg/100mL ) ratio [(Z)/(Y)] is preferably 7.3 or less, more preferably 7.1 or less, still more preferably 6.9 or less, still more preferably 6.7 or less, still more preferably 6.5 or less, particularly preferably 6.0 or less, Moreover, it may be 5.9 or less, 5.8 or less, 5.7 or less, 5.6 or less, or 5.5 or less.

The beer-flavored beverage of one aspect of the present invention contains phosphoric acid, lactic acid or salts thereof. Although it has no special effect on suppressing the satiety that is not suitable for beer-flavored beverages, it lowers the pH to ensure microbial protection and does not impart any contamination to the beverage. Unsuitable sourness is possible.

In order not to impart unsuitable sourness to the beer-flavored beverage, in the beer-flavored beverage according to one aspect of the present invention, the phosphoric acid content is preferably 800 mass ppm or less, more preferably 750 mass ppm or less, further preferably 700 mass ppm or less, and 600 mass ppm. Below is better, below 550 ppm by mass is even better, below 500 ppm by mass is even better, below 450 ppm by mass is even better, below 400 ppm by mass is even better, below 300 ppm by mass is even better, below 200 ppm by mass is especially good . In addition, in order to lower the pH of the beer-flavored beverage and obtain microbial protection, in the beer-flavored beverage according to one aspect of the present invention, the phosphoric acid content is preferably 40 mass ppm or more, more preferably 50 mass ppm or more, and still more preferably 60 mass ppm or more. 70 quality ppm or above is better.

Similarly, in order not to impart an unsuitable sourness to the beer-flavored beverage, in the beer-flavored beverage according to one aspect of the present invention, the lactic acid content is preferably 950 mass ppm or less, more preferably 900 mass ppm or less, and still more preferably 800 mass ppm or less. 700 mass ppm or less is better, 600 mass ppm or less is even better, 500 mass ppm or less is even better, 450 mass ppm or less is even better, 400 mass ppm or less is even better, 300 mass ppm or less is even better, 200 mass ppm is even better The following are particularly good. In addition, in order to lower the pH of the beer-flavored beverage and obtain microbial protection, the content of lactic acid in the beer-flavored beverage according to one aspect of the present invention is preferably 40 mass ppm or more, more preferably 50 mass ppm or more, and still more preferably 60 mass ppm or more. , 70 quality ppm or above is better.

In the beer-flavored beverage according to one aspect of the present invention, the contents of phosphoric acid and lactic acid can be controlled, for example, by adjusting the usage amount of raw materials containing a large amount of phosphoric acid or lactic acid. In addition, the content of lactic acid and phosphoric acid in the beer-flavored beverage of the present invention can be measured, for example, by high-speed liquid chromatography analysis.

From the viewpoint of making the beer-flavored beverage easy to drink, the sugar concentration of the beer-flavored beverage according to one aspect of the present invention is preferably 6.0g/100mL or less, more preferably 5.5g/100mL or less, and even more preferably 5.0 g/100mL or less, more preferably 4.8g/100mL or less, still more preferably 4.6g/100mL or less, still more preferably 4.4g/100mL or less, still more preferably 4.3g/100mL or less, particularly preferably 4.2g/100mL or less, and further, it may be 4.0g/100mL or less, 3.8g/100mL or less, 3.6g/100mL or less, 3.5g/100mL or less, 3.3g/100mL or less, 3.2g/100mL or less, or 3.0g/100mL or less. In addition, the sugar concentration of the beer-flavored beverage according to one aspect of the present invention is preferably 0.3g/100mL or more, more preferably 0.5g/100mL or more, and more preferably 0.5g/100mL or more, from the viewpoint of making it a beer-flavored beverage with a drinking sensation. Preferably it is 0.8g/100mL or more, further preferably 1.0g/100mL or more, further preferably 1.3g/100mL or more, still more preferably 1.5g/100mL or more, still more preferably 1.7g/100mL or more, particularly preferably 1.8 g/100mL or more, and further, it may be 2.0g/100mL or more, 2.2g/100mL or more, or 2.5g/100mL or more.

In addition, in this manual, the so-called "carbohydrate" refers to the nutritional labeling standards for foods (Ministry of Health, Labor and Welfare Notice No. 176, 2015, partially revised Consumer Advertising Notice No. 8, September 27, 2015). Specifically, sugar refers to what is obtained by removing protein, lipid, dietary fiber, ash, alcohol and water from the target food. Therefore, the sugar content in food can be calculated by deducting the amount of protein, lipid, dietary fiber, ash and moisture from the weight of the food. Here, the amounts of protein, lipid, dietary fiber, ash and moisture can be measured according to the methods disclosed in the Nutrition Labeling Standards. Specifically, the amount of protein can be measured by the nitrogen quantitative conversion method, the amount of lipid can be measured by the ether extraction method, the amount of dietary fiber can be measured by the Prosky method, and the amount of ash can be measured by the direct ash extraction method. The amount of moisture can be measured by the reduced pressure heating and drying method.

In addition, the sugar content of the beer-flavored beverage according to one aspect of the present invention can be adjusted by appropriately setting the addition of diluting water or carbonated water, the type of enzyme, the amount of enzyme added, the time point of addition, and when preparing the saccharification liquid. The set temperature and residence time of each temperature zone, the composition of the pre-fermentation liquid (original extract concentration, sugar composition, protein content, dietary fiber content, ash content, etc.), and the various conditions of the fermentation step (oxygen concentration, aeration conditions, yeast species , yeast addition amount, yeast proliferation number, yeast removal time, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, enzyme type, enzyme addition amount, enzyme addition time, etc.), cooling time, cooling Temperature, cooling time, etc. can be adjusted.

The color of the beer-flavored beverage according to one aspect of the present invention is preferably 5.0 EBC or more, more preferably 5.3 EBC or more, and even more preferably 5.6 EBC or more, from the viewpoint of becoming a beer-flavored beverage with a strong impact when drinking. , further preferably above 5.9EBC, particularly preferably above 6.2EBC, and may also be above 6.5EBC, above 7.0EBC, above 7.5EBC, or above 8.0EBC. Furthermore, the color of the beer-flavored beverage according to one aspect of the present invention is preferably 20.0EBC or less, more preferably 19.0EBC or less, further preferably 18.0EBC or less, still more preferably 17.0EBC or less, still more preferably 16.0EBC or less. or less, more preferably 15.0 EBC or less, still more preferably 14.0 EBC or less, and may also be 13.0 EBC or less, 12.0 EBC or less, or 10.0 EBC or less.

In this manual, the "color" of the beverage can be determined by the "8.8 color" of the revised BCOJ Beer Analysis Method (issued by the Japan Brewing Association, edited and revised in 2013 by the International Technical Committee of the Beer Brewers Association [Analysis Committee]) Measured according to the prescribed measurement method. In addition, the "color" of a beverage is defined by the color unit (EBC unit) established by the European Brewery Convention. The smaller the numerical value, the lighter and brighter the color of the drink, and conversely, the larger the numerical value, the thicker and darker the color of the beverage. Furthermore, the color of the beer-flavored beverage according to one aspect of the present invention can be achieved by appropriately adjusting the type of malt used, the blending ratio when two or more types of malt are used together, and the mixing ratio when preparing the pre-fermentation liquid. Boiling conditions, etc. are controlled. More specifically, for example, the color of beer-flavored beverages can be improved by increasing the blending ratio of rich colored malt as malt, increasing the temperature during boiling, lengthening the boiling time, and decoction when preparing the saccharification liquid. Wait to adjust. In addition, the color can also be increased by increasing the concentration of the original wort extract or increasing the malt ratio. It can also be adjusted by controlling the amount of food additives such as caramel coloring or colored sugar liquid.

Although the pH of the beer-flavored beverage according to one aspect of the present invention is not particularly limited, from the viewpoint of improving the flavor of the beverage, it is preferably 3.0 or more, more preferably 3.2 or more, further preferably 3.4 or more, and even more preferably It is 3.6 or more, more preferably 3.7 or more, and it can also be 3.9 or more, or 4.1 or more. Moreover, from the viewpoint of inhibiting the generation of microorganisms, the pH of the beer-flavored beverage is preferably 5.0 or less, more preferably 4.9 or less, further preferably 4.8 or less, still more preferably 4.7 or less, and particularly preferably 4.6 or less. The pH can be adjusted by appropriately setting the addition of dilution water or carbonated water, the type of raw materials (malt, ground corn, sugar solution, etc.), the amount of raw materials, the type of enzyme, the amount of enzyme added, and the time point of adding enzyme. , the saccharification time in the feed trough, the protein decomposition time in the feed trough, the pH in the feed trough, the pH in the feeding step (the wort manufacturing step before adding malt to yeast), and is used for pH adjustment. The type of acid (lactic acid, phosphoric acid, malic acid, tartaric acid, citric acid, etc.), the amount of acid added when adjusting the pH, and the time point of the pH adjustment (when feeding, during fermentation, at the end of fermentation, before beer filtration, After beer filtration, etc.), the set temperature and residence time of each temperature zone when preparing wort (including saccharification), the original extract concentration of the pre-fermentation liquid, the original extract concentration in the fermentation step, fermentation conditions (oxygen concentration, Aeration conditions, yeast species, yeast addition amount, yeast proliferation number, yeast removal time, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.), cooling time, cooling temperature, cooling time, etc.

The beer-flavored beverage according to one aspect of the present invention is suitable for packaging in a container. Examples of the container include bottles, plastic bottles, cans, and barrels. However, particularly from the viewpoint of portability, cans, bottles, and plastic bottles are preferred. In addition, when using colorless transparent bottles or plastic bottles, they will be exposed to sunlight or fluorescent light unlike ordinary cans or colored bottles.

The details of optional additive raw materials such as cereals and sweeteners that can be used in the production of the beer-flavored beverage of the present invention are described in "1.1 Raw Materials".

1.1 Raw materials Although the main raw materials of the beer-flavored beverage according to one aspect of the present invention are water and malt, hops are preferably used. In addition, sweeteners, water-soluble dietary fiber, bitters or bitterness-imparting agents, antioxidants, etc. may also be used. Oxidants, spices, sour ingredients, salts, etc.

The term "malt" means those obtained by germinating, drying, and removing the roots of wheat seeds such as barley, wheat, rye, oats, oats, coix, oats, etc. The origin or variety may be any one. In one aspect of the present invention, barley malt is preferably used. Barley malt is the most commonly used malt as a raw material for beer-flavored beverages in Japan. Among barley, there are types such as two-row barley and six-row barley, but either type can be used. Furthermore, in addition to ordinary malt, colored malt, etc. can also be used. In addition, when using colored malt, different types of colored malt may be appropriately combined, or one type of colored malt may be used.

The malt used in the beer-flavored beverage of the present invention preferably has a modification degree of 80% or more. If the modification degree is less than 80%, the viscosity of the wort will increase, or the turbidity will increase, and the production efficiency such as wort filterability and beer filterability will deteriorate. Therefore, it is better to use malt with a modification degree of 80% or more. In the examples and comparative examples described below, malt with a modification degree of 80% or more is used. The degree of modification can be recorded by MEBAK Raw Materials Barley Adjuncts Malt Hops And Hop Products Published by the Chairman Dr.Fritz Jacob Self-published by MEBAK 85350 Freising-Weihenstephan, Germany 2011 3.1.3.8 Modification and Homogeneity (Calcofluor Carlsberg Method-EBC) method to measure. In addition, in the beer-flavored beverage of one aspect of the present invention, the malt used is appropriately selected depending on the desired color of the beer-flavored beverage. The selected malts may be used alone or two or more types may be used in combination.

Malt contains proline, pyroglutamic acid and polyphenols. Therefore, in the present invention, in order to make the proline, pyroglutamic acid, and total polyphenol amounts of the beer-flavored beverage of the present invention fall within the range specified by the present invention, the ratio of malt in the raw materials is set to a certain value. Better range. Specifically, the malt ratio (the ratio of all malts used) is preferably 40 mass% or more, more preferably 45 mass% or more, further preferably 48 mass% or more, still more preferably 50 mass% or more, especially Preferably it is 55 mass% or more, and it may be 60 mass% or more, 65 mass% or more, 66 mass% or more, 67 mass% or more, 70 mass% or more, 80 mass% or more, 90 mass% or more or 100 mass%. . By increasing the malt ratio, you can create a beer-flavored beverage in which the rich taste derived from malt or the umami flavor of malt can be felt more strongly. In addition, if the malt ratio is too high, an uncomfortable feeling of fullness is likely to occur, so it is preferably 90 mass% or less, more preferably 85 mass% or less, still more preferably 80 mass% or less, and still more preferably 78 mass%. or less, preferably 76 mass% or less, still more preferably 74 mass% or less, still more preferably 72 mass% or less, still more preferably 70 mass% or less, or 68 mass% or less, and 67 mass% or less. % or less, or 66.6 mass% or less. In this manual, the malt ratio refers to the value calculated in accordance with the Liquor Tax Act and the Liquor Administrative Relations Act, which came into effect on April 1, 2011.

When suppressing the malt ratio, it is better to increase the amount of raw materials (carbon source, nitrogen source) other than malt that the yeast can assimilate. Examples of carbon sources that can be assimilated by yeast include monosaccharides, disaccharides, trisaccharides, sugar liquids thereof, liquid sugars containing carbon sources, etc. Examples of nitrogen sources include yeast extract, soybean protein, malt, and soybeans. , yeast extract, peas, wheat malt, ungerminated grains, their decomposition products, etc. Examples of ungerminated grains include ungerminated barley, wheat, rye, oats, oats, job's tears, oats, rice (white rice, black rice, etc.), corn, sorghum, potatoes, beans (soybeans, peas, etc.), Buckwheat, millet, millet, barnyard grass, etc. In addition, starch obtained from these cereals and their extracts can also be used.

Fruits, peels, barks, leaves, flowers, stems, roots, and seeds of plants other than wheat and other grass plants that can be used as raw materials can be appropriately selected. Specific examples of plants other than grass plants include citrus, soft fruits, herbs, spices, and the like. Examples of citrus include orange, pomelo, lemon, lime, tangerine, grapefruit, yoko, kumquat, orange, lime, Taiwanese lemon, sorrel, and the like. Examples of soft fruits include peaches, grapes, bananas, apples, grapes, pineapples, strawberries, pears, muscadines, black currants, and the like. Examples of herbs and spices include coriander, pepper, cumin, Sichuan peppercorns, sansho pepper, cardamom, fennel, nutmeg, nutmeg bark, juniper berries, allspice, vanilla, elderberry, paprika, anise, Star anise, etc. These may be used directly or crushed, may be used in the form of an extract extracted with an extraction solvent such as water or ethanol, or may be used after squeezed juice (juice, etc.). These may be used individually or in combination of 2 or more types. Although the above can be used appropriately according to the consumer's preferences, in order to enjoy the refreshing taste like beer, the above-mentioned citrus, soft fruits, herbs, and spices are not used as raw materials at all, or the usage amount is set to Minimum is better. In particular, blackcurrants have a milky taste that is not suitable for beer, so it is better not to use blackcurrants or blackcurrant juice in the raw materials, or to minimize the amount used.

Examples of the form of hops used in one aspect of the present invention include pellet hops, powdered hops, and hop extracts. In addition, the hops used may also be hop processed products such as alienated hops and reduced hops. The amount of hops added can be appropriately adjusted, but is preferably 0.0001 to 1% by mass relative to the total amount of the beverage. In addition, a beer-flavored beverage using hops as a raw material contains iso-alpha acid, a component derived from hops.

Although the bitterness value of the beer-flavored beverage of the present invention is not particularly limited, it is preferably 5.0 BUs or more and 45.0 BUs or less. In this specification, the so-called "bitterness value" is an indicator of the bitterness caused by iso-α acids such as isohumulone. The bitterness value can be determined by following the method described in "BCOJ Beer Analysis Method (2004.11.1 Revision) 8.15 Bitterness Value". Specifically, acid can be added to the degassed sample and then extracted with isooctane. The absorbance of the isooctane layer measured at 275 nm against isooctane is multiplied by a coefficient to obtain the bitterness value (BUs). The bitterness value depends on the content of iso-alpha acid contained in the beverage. Iso-alpha acid is a bitter component contained in large amounts in hops. Therefore, by controlling the amount of hops used, a beverage having a specified bitterness value can be produced.

Examples of sweeteners include commercially available saccharified liquids obtained by decomposing starch derived from cereals with acids or enzymes, sugars such as sucrose, commercially available starch syrup, trisaccharides or higher sugars, sugar alcohols, and isomerized sugars. Natural sweeteners, artificial sweeteners, etc. such as sugar and stevia. The form of these sugars may be liquid such as solution, or solid such as powder. In addition, there are no particular restrictions on the type of starch raw material grains, starch purification methods, and processing conditions such as hydrolysis using enzymes or acids. For example, it is also possible to use sugars that increase the ratio of maltose by appropriately setting the conditions for hydrolysis using enzymes or acids. In addition, sucrose, fructose, glucose, maltose, trehalose, maltotriose, maltotetraose, isomaltose, isomaltotriose, isomaltotetraose and their solutions (sugar liquids) can also be used. . Examples of artificial sweeteners include aspartame, acesulfame potassium (acesulfame K), sucralose, and the like. These sweeteners may be used alone or two or more types may be used in combination.

Examples of the water-soluble dietary fiber include indigestible dextrin, polydextrose, swan gum decompose product, pectin, glucomannan, alginic acid, laminarin, fucoidan, carageenan, etc. From the viewpoint of versatility such as stability and safety, indigestible dextrin or polydextrose is preferred.

In beer-flavored beverages, bitterness is preferably imparted by hops, etc., but bittering materials or bitterness-imparting agents may also be used. There are no particular limitations on the bitterness agent or bitterness-imparting agent. Those commonly used as bitterness-imparting agents in beer or sparkling wine can be used. Examples include rosemary, lychee, anise, juniper, sage, and rosemary. Incense, Ganoderma lucidum, laurel, Ganoderma lucidum, quassersin, citrus extract, quassara extract, coffee extract, tea extract, bitter melon extract, lotus germ extract, aloe vera extract, rosemary extract, lychee Extract, bay extract, sage extract, Tibetan fennel extract, wormwood extract, absinthe, alginic acid, etc.

The antioxidant is not particularly limited, and those commonly used as antioxidants in beer or sparkling wine can be used. Examples thereof include ascorbic acid, erythorbic acid, catechin, and the like.

The flavoring is not particularly limited, and general beer flavoring can be used. Beer flavors are used to add a beer-like flavor and include brewing ingredients produced through fermentation. In addition, beer-flavored beverages contain ethyl acetate produced by alcohol fermentation, but this ethyl acetate has a function as a flavor. Therefore, in the case where alcoholic fermentation is accompanied during the manufacturing process of beer-flavored beverages, although the necessity of additionally adding beer flavoring is low, it may be desired to add beer flavoring. Examples of beer flavors other than ethyl acetate include esters, higher alcohols, etc. Specific examples include isoamyl acetate, ethyl acetate, n-propanol, isobutanol, acetaldehyde, ethyl hexanoate, and caprylic acid. Ethyl ester, isopentylpropionate, linalol, geraniol, citral, 4-vinylguaiacol (4-VG), 4-methyl-3-pentenoic acid, 2-methyl- 2-Pentenoic acid, 1,4-cineole, 1,8-cineole, 2,3-diethyl-5-methylpyridine , γ-decanolide, γ-undecylactone, ethyl caproate, 2-methylethyl butyrate, n-ethyl butyrate, myrcene, citral, limonene, maltol, ethyl malt Alcohol, phenylacetic acid, furanone, furfural, methylthiopropionaldehyde, 3-methyl-2-butene-1-thiol, 3-methyl-2-butanethiol, diacetyl, ferulic acid , geranic acid, geranyl acetate, ethyl butyrate, caprylic acid, capric acid, 9-decenoic acid, nonanoic acid, myristate, propanoic acid, 2-methylpropanoic acid, γ-butyrolactone, 2-amine Acetophenone, ethyl 3-phenylpropionate, 2-ethyl-4-hydroxy-5-methyl-3(2H)-furanone, dimethylsulfone, 3-methylcyclopentane-1 ,2-diketone, 2-methylbutanal, 3-methylbutanal, 2-methyltetrahydrofuran-3-one, 2-acetylfuran, 2-methyltetrahydrofuran-3-one, hexanal, Hexanol, cis-3-hexenal, 1-octen-3-ol, β-cineol, 4-mercapto-4-methylpentan-2-one, β-caryophyllene, β-lauric oil Alkene, furfuryl alcohol, 2-ethylpyra , 2,3-dimethylpyridine , 2-methylbutyl acetate, isopentyl alcohol, 5-hydroxymethylfurfural, phenyl acetaldehyde, 1-phenyl-3-buten-1-one, trans-2-hexenal, nonanal , phenylethyl alcohol. These spices can be used alone or two or more kinds can be used in combination.

The sour agent is not particularly limited as long as it has a sour taste, but examples thereof include phosphoric acid, citric acid, gluconic acid, tartaric acid, lactic acid, malic acid, phytic acid, acetic acid, succinic acid, gluconolactone, and the like. of salt. Among these sour ingredients, phosphoric acid, citric acid, gluconic acid, tartaric acid, lactic acid, malic acid, phytic acid, acetic acid, succinic acid or their salts are preferred, and phosphoric acid, citric acid, lactic acid, tartaric acid, acetic acid or their salts are preferred. These salts are more preferred, and phosphoric acid, lactic acid or salts thereof are particularly preferred. These sour ingredients can be used alone or two or more kinds can be used in combination. The content of phosphoric acid, lactic acid or these salts is as described in the above "1. Beer flavored beverage".

Examples of the preservation material include benzoic acid; benzoates such as sodium benzoate; benzoate esters such as propyl parahydroxybenzoate and butyl parahydroxybenzoate; dimethyl dicarbonate and the like. In addition, as a preservation material, commercially available preparations such as Power Sanpreser (manufactured by Saneigen F.F.I. Co., Ltd., a mixture of sodium benzoate and butyl benzoate) can also be used. These preservation materials can be used alone or two or more types can be used in combination. The blending amount of the preservation material is preferably 5 to 1200 mass ppm, more preferably 10 to 1100 mass ppm, further preferably 15 to 1000 mass ppm, and still more preferably 20 to 900 mass ppm.

Examples of salts include sodium chloride, acidic potassium phosphate, acidic calcium phosphate, ammonium phosphate, magnesium sulfate, calcium sulfate, potassium metabisulfite, calcium chloride, magnesium chloride, potassium nitrate, ammonium sulfate, potassium chloride, Monosodium citrate, disodium citrate, trisodium citrate, etc. These salts can be used alone or two or more kinds can be used in combination.

1.2 Carbon dioxide gas The carbon dioxide gas contained in the beer-flavored beverage according to one aspect of the present invention can utilize the carbon dioxide gas contained in the raw materials, and can be dissolved by being miscible with carbonated water or adding carbon dioxide gas. Since the beer-flavored beverage of one aspect of the present invention undergoes alcoholic fermentation, the carbon dioxide gas generated in this fermentation step can be directly used, but carbonated water can also be appropriately added to modulate the amount of carbon dioxide gas.

The carbon dioxide gas concentration of the beer-flavored beverage according to one aspect of the present invention is preferably 0.30 (w/w)% or more, more preferably 0.35 (w/w)% or more, and even more preferably 0.40 (w/w)% or more. , more preferably 0.42 (w/w)% or more, particularly preferably 0.45 (w/w)% or more, and more preferably 0.80 (w/w)% or less, more preferably 0.70 (w/w)% or less, more preferably 0.60 (w/w)% or less, still more preferably 0.57 (w/w) or less, particularly preferably 0.55 (w/w)% or less. In addition, in this specification, the carbon dioxide gas concentration can be determined by immersing the container containing the target beverage in a water tank at 20°C for more than 30 minutes while vibrating from time to time. After the beverage is adjusted to 20°C, a gas volume measuring device (such as , GVA-500 (manufactured by Kyoto Electronics Industry Co., Ltd., etc.) to measure.

When the beer-flavored beverage according to one aspect of the present invention is a container-packed beverage, the carbon dioxide gas pressure of the container-packed beverage can be appropriately adjusted within the range of the above-mentioned carbon dioxide gas concentration. However, the carbon dioxide gas pressure of the beverage may be 5.0 kg. /cm ² or less, 4.5kg/cm ² or less, or 4.0kg/cm ² or less, and 0.20kg/cm ² or more, 0.50kg/cm ² or more, or 1.0kg/cm ² or more. These upper limits can be combined and any of the lower limits. For example, the carbon dioxide pressure of a beverage can be 0.20kg/ ^cm2 or more and 5.0kg/ ^{cm2 or} less, 0.50kg/ ^cm2 or more and 4.5kg/ ^{cm2 or} less, or 1.0kg/ ^cm2 or more and 4.0kg/ ^cm2 or less. In this manual, the so-called air pressure refers to the air pressure inside the container, except for special circumstances. The pressure can be measured using methods well known to those in the technical field. For example, after fixing a sample at 20°C to a gas internal pressure gauge, open the stopcock of the gas internal pressure gauge once to release the gas, close the stopcock again, and vibrate the gas internal pressure. The method is to measure and read the value when the pointer reaches a certain position, or you can use a commercially available air pressure measuring device to measure it.

1.3 Other additives To the beer-flavored beverage of one aspect of the present invention, various additives may be added as necessary within the scope that does not hinder the effect of the present invention. Examples of such additives include coloring materials, foam forming agents, fermentation accelerators, yeast extracts, protein-based substances such as peptide-containing substances, and seasonings such as amino acids. Colorants used to give beverages a beer-like color, such as caramel pigments, can be used. Foam forming agents are used to form beer-like foam in beverages or to maintain foam in beverages. Plant-extracted saponin-based substances such as soybean saponin and Quillaja bark saponin, and plant proteins such as corn and soybean can be appropriately used. , and things containing peptides such as collagen peptides, yeast extract, raw materials derived from milk, etc. The fermentation accelerator is used to promote fermentation using yeast, and can be used alone or in combination, for example, yeast extract, rice or wheat bran components, vitamins, mineral agents, etc.

1.4 Containerized beverages The beer-flavored beverage according to one aspect of the present invention may be a container-packed beverage packaged in a container. Containerized beverages can use containers of any shape and material. Examples of containers include bottles, cans, buckets, and plastic bottles. However, especially from the viewpoint of portability, cans, bottles, and plastic bottles are Better.

2. Manufacturing method of beer-flavored beverage The present invention also relates to a method for producing beer-flavored beverages. The manufacturing method of one aspect of the present invention is specifically characterized in that the total polyphenol content is 60 to 200 ppm by mass, the proline content is 15 to 75 mg/100 mL, and the pyroglutamic acid content is 50 to 300 mg. /L, the apparent fermentation degree is set as 70.0~95.0%. Furthermore, the manufacturing method according to one aspect of the present invention may include the following steps (1) to (3) more specifically. ・Step (1): The step of performing at least one of saccharification treatment, boiling treatment, and solid content removal treatment on the raw materials to obtain a pre-fermentation liquid. ・Step (2): The step of cooling the pre-fermentation liquid obtained in step (1) to obtain a cooled pre-fermentation liquid. ・Step (3): Add yeast to the cooled pre-fermentation liquid obtained in step (2) to perform alcoholic fermentation.

In addition, the manufacturing method of one aspect of the present invention may also include, in addition to the above steps (1) to (3), a step of confirming and/or adjusting the total polyphenol content and the contents of proline and pyroglutamic acid. This step will be described later as step (4).

＜Step (1)＞ Step (1) is a step of using various raw materials to perform at least one of saccharification treatment, boiling treatment and solid content removal treatment to obtain a pre-fermentation liquid. For example, when malt is used as various raw materials, various raw materials including water and malt are put into a feed kettle or a feed tank, and polysaccharide decomposing enzymes or polysaccharide decomposing enzymes that promote changes in components derived from the raw materials may be added before fermentation if necessary. Enzymes such as proteolytic enzymes. Examples of the enzyme agent include amylase, protease, purine nucleosidase, deaminase, polyphenol oxidase, polyglucose, xylase, pectinase, cellulase, lipase, and glucosidase. Xanthine oxidase, transglucosidase, etc. In addition, for the purpose of "(3) Rationalization of brewing, etc." of Article 3 "7 Items not to be treated as raw materials for alcoholic beverages" of the Liquor Tax Act and Liquor Administrative Relations Ordinance (revised on June 27, 2010), the brewing process can be used as an example The following enzymes added in "Enzymes". By adding these enzymes, the composition of the resulting beer-flavored beverage can be efficiently adjusted. As various raw materials other than malt, hops, preservatives, sweeteners, water-soluble dietary fibers, bitters or bitterness-imparting agents, antioxidants, spices, sour agents, salts, etc. can also be added. These can be added before the saccharification process, during the saccharification process, or after the saccharification process is completed. Moreover, these can also be added during the alcoholic fermentation in the subsequent step or after the alcoholic fermentation.

The mixture of various raw materials is heated to saccharify the starch of the raw materials and perform saccharification process. The temperature and time of the saccharification process should be adjusted appropriately, taking into account the type of malt used, or malt ratio, raw materials other than water and malt, the type or amount of enzyme used, the original extract concentration of the final beverage, etc. good. In one aspect of the present invention, from the viewpoint of adjusting the apparent fermentation degree of the beer-flavored beverage to the above range, the temperature of the saccharification treatment is 55 to 75°C, and the time of the saccharification treatment is preferably 15 to 240 minutes. After the saccharification process, filter the saccharified liquid to obtain the saccharified liquid.

In addition, it is better to boil this saccharified liquid. When performing this boiling process, when using hops, bitters, etc. as raw materials, it is preferable to add these. Hops or bitters, etc., can be added from the beginning to the end of boiling of the saccharified liquid. In addition, instead of the above-mentioned saccharified liquid, warm water may be added to the malt extract, hops or bitters, etc. may be added and boiled to prepare a pre-fermentation liquid.

In addition, when malt is not used as various raw materials, liquid sugar containing carbon sources, wheat, or other materials other than malt may be used as nitrogen sources, hops, preservatives, sweeteners, and water-soluble amino acid-containing raw materials. Dietary fiber, bitterness or bitterness imparting agent, antioxidant, spice, sourness, salt, etc. are mixed with warm water to prepare a liquid sugar solution, and the liquid sugar solution is boiled to prepare a pre-fermentation liquid. When using hops, it can be added before boiling, or it can be added between the start of boiling and the end of boiling of the liquid sugar solution.

＜Step (2)＞ Step (2) is a step of cooling the pre-fermentation liquid obtained in step (1) to obtain a cooled pre-fermentation liquid. After the boiling process is completed, transfer to a whirlpool bath and cool to 0~23°C. Then, after cooling, solid components such as coagulated protein may be removed to adjust the original extract concentration. After such treatment, a cooled fermentation pre-liquid can be obtained.

＜Step (3)＞ Step (3) is a step of adding yeast to the cooled pre-fermentation liquid obtained in step (2) to perform alcoholic fermentation. The yeast used in this step can be appropriately selected taking into account the type of fermented beverage to be produced, the target flavor, or the fermentation conditions. Top-fermenting yeast or bottom-fermenting yeast can be used.

For yeast, yeast suspension can be added directly to the raw materials, or a slurry obtained by centrifugal separation or sedimentation and concentration of yeast can be added to the pre-fermentation liquid. Alternatively, it can be added after centrifugation and the supernatant is completely removed. The amount of yeast added to the stock solution can be appropriately set, but is, for example, about 5×10 ⁶ cells/mL to 1×10 ⁸ cells/mL.

Various conditions such as fermentation temperature and fermentation period during alcohol fermentation can be set appropriately. However, from the perspective of adjusting the degree of apparent fermentation, for example, fermentation can be made faster at 8 to 25°C for 5 to 10 days. good. The temperature (heating or cooling) or pressure of the fermentation liquid can also be changed during the fermentation step. The apparent degree of fermentation of beer-flavored beverages can be adjusted by appropriately setting the type, amount and timing of polysaccharide decomposing enzymes such as transglucosidase, and by changing the fermentation liquid during the fermentation step. Temperature (heating or cooling) or pressure to adjust. In addition, after this step is completed, the yeast can be removed with a filter, etc., and water or additives such as spices, sours, and pigments can be added as needed. In this way, the apparent degree of fermentation of the resulting beer-flavored beverage can be adjusted to a desired range by appropriately adjusting the conditions of the saccharification treatment in step (1) and the alcoholic fermentation in step (3).

＜Step (4)＞ Step (4) is a step of confirming and/or adjusting the total polyphenol content, proline and pyroglutamic acid content. The content of these ingredients can also be determined by appropriately setting the types of raw materials or their blending amounts, feeding conditions (time of adding raw materials, etc.), yeast species, or fermentation conditions in steps (1) and (2). Wait to adjust. Therefore, it is better to measure the content of these components in step (4) and confirm that it is within the above range. Then, if there is a component outside the range among these components, it is better to adjust by adding the component outside the range or diluting it.

In addition, the adjustment of the content of each component in this step can be performed in parallel with step (1), step (2) and/or step (3), can be performed between step (1) and step (2), or can be It can be carried out between step (2) and step (3), or it can also be carried out after step (3). In addition, although the confirmation of the content of each component in this step can be carried out at any time point mentioned above, the content of each component should be confirmed after step (3). If there are any components that need to be adjusted, the It is better to adjust the content of this ingredient.

After these steps, you may also perform steps such as a wine storage step and a filtration step that are well known to those skilled in the art in the production of beer-flavored beverages. The beer-flavored beverage obtained in this manner is filled into a designated container and distributed in the market as a product. There are no particular limitations on the container packaging method for the beer-flavored beverage, and any container packaging method well known to those in the technical field can be used. Through the container packaging step, the beer-flavored beverage can be filled and sealed in the container. In the container packaging step, containers of any shape and material can be used as examples of containers, as mentioned above.

3. Methods to improve the aroma of beer-flavored beverages The present invention also relates to a method for improving the aroma of beer-flavored beverages. The aroma improvement method of one aspect of the present invention is characterized in that the total polyphenol content is 60 to 200 ppm by mass, the proline content is 15 to 75 mg/100 mL, and the pyroglutamic acid content is 50 For beer-flavored beverages with ~300 mg/L, the apparent fermentation degree is adjusted so that the apparent fermentation degree becomes 70.0~95.0%. Another aspect of the aroma improvement method of the present invention is to obtain a beer-flavored beverage with an apparent degree of fermentation of 70.0-95.0% so that the total polyphenol content is 60-200 mass ppm and the proline content is 15-75 mg. /100mL, adjust the total polyphenols, proline and pyroglutamic acid content in such a way that the pyroglutamic acid content is 50~300mg/L.

In this specification, the so-called "flavor" of beer-flavored beverages includes the umami flavor of wheat, the rich taste derived from wheat, the drinking feeling, and the mellow taste of the beer. In addition, in this specification, the so-called "flavor improvement" or "flavor improvement" means a beverage in which the proline content, pyroglutamic acid content, total polyphenol content, and apparent degree of fermentation are adjusted to the above ranges, and a beverage in which this process is not performed. Compared with the adjusted beverage, at least one of reducing or suppressing the unpleasant bland taste, increasing the rich taste derived from wheat, increasing the drinking feeling, and increasing the mellow taste of the taste is achieved.

Adjust the optimal ranges of proline content, pyroglutamic acid content, total polyphenol content, and apparent degree of fermentation, as well as the methods for these contents, such as the above "1. Beer-flavored beverage" and the above-mentioned "2. Beer flavor" "Beverage Manufacturing Method". [Example]

Hereinafter, although the present invention will be further described in detail through examples and the like, the present invention is not limited by these examples. In addition, in the examples, the original extract concentration and the total polyphenol content are based on the method described in the revised BCOJ Beer Analysis Method (published by the Japan Brewing Association, compiled by the International Technical Committee of the Beer Brewing Association [Analysis Committee] in 2013). to measure.

＜Preparation of drinks＞ After the crushed barley malt is put into a feed tank filled with 120L of warm water, the temperature is gradually increased and maintained, and the malt meal, etc. are filtered and removed. Moreover, as described in Tables 1 to 3, in some embodiments, proteolytic enzymes are added to warm water together with barley malt. After filtration, the raw material liquid and hops were put into a boiling kettle and adjusted to 100L with warm water to obtain hot wort. The obtained hot wort was cooled and aerated with oxygen to obtain 60 L of pre-fermentation liquid before adding yeast.

Brewer's yeast (lower fermentation yeast) is added to the pre-fermentation liquid obtained in this way and fermented for about one week. After a maturation period of about one week, the yeast is removed by filtration, and the extract is added to adjust water to prepare a beer-flavored beverage. In each of the Examples and Comparative Examples, the amount or type of raw materials such as malt, liquid sugar, hops, etc., the mashing mode, the type of proteolytic enzyme, the amount and time of addition, and the preparation were appropriately set. The set temperature of each temperature zone during wort, residence time, pH adjustment, turbidity during wort filtration, hop addition time, boiling time, fermentation conditions, etc., are used to form the apparent fermentation degree shown in Tables 1 to 3 , proline content, pyroglutamic acid content, total polyphenol content, original extract concentration, sugar content, color, pH, lactic acid and phosphoric acid content. In addition, in Reference Examples 1 to 8 described in Table 1, the sugar content, color, pH, pyroglutamic acid, lactic acid and phosphoric acid contents were not adjusted and measured.

＜Sensory evaluation＞ The evaluation of the obtained beer-flavored beverages was carried out by the same 6 sensory inspectors who tasted each beverage as follows.

Each sensory inspector tried to drink 350 mL of beer-flavored beverages cooled to about 4°C. Regarding "the satiety that is not suitable for beer-flavored beverages", "the bland taste that is not suitable for beer-flavored beverages" and "the richness that is suitable for beer-flavored beverages" The evaluation item "Taste" is evaluated based on the following score standards, ranging from 3.0 (maximum value) to 1.0 (minimum value), with a score of 0.1 scale, and the average score of the 6 sensory inspectors is calculated. In addition, in Reference Examples 1 to 8 described in Table 1, the evaluation item of "rich taste suitable for beer-flavored beverages" was not evaluated. During the evaluation, samples are prepared in advance in which the evaluation items meet the following standards "1.0", "2.0" and "3.0" respectively, in an attempt to unify the standards among various functional inspectors. Moreover, in the sensory evaluation of all Examples and Comparative Examples, for the same beverage, no difference in score value of 2.0 or more between each sensory examiner was confirmed.

[Not suitable for satiety of beer-flavored drinks] ・"3.0": I feel uncomfortable and do not feel full at all after drinking. ・"2.5": Uncomfortable and not feeling full after drinking. ・"2.0": I feel uncomfortable and barely feel full after drinking. ・"1.5": Uncomfortable feeling of fullness after drinking. ・"1.0": Uncomfortable and strong feeling of fullness after drinking. [Not suitable for beer-flavored beverages with bland taste] ・"3.0": The taste is not bland at all and is not suitable for beer-flavored drinks. ・"2.5": There is no bland taste that is not suitable for beer-flavored drinks. ・"2.0": There is almost no taste that is not suitable for beer-flavored drinks. ・"1.5": The taste is too bland and not suitable for beer-flavored drinks. ・"1.0": I strongly feel that it is not suitable for beer-flavored drinks because of the bland taste. [Suitable for rich taste of beer-flavored drinks] ・"3.0": The rich taste of the beer-like drink is very strong. ・「2.5」: The rich taste of beer-like beverage is strongly felt. ・"2.0": Feel the rich taste of beer-flavored drinks. ・"1.5": I don't feel the strong taste of beer-flavored drinks. ・"1.0": There is almost no strong taste like beer-flavored drinks.

In addition, based on the above three evaluation items, a total evaluation is performed based on the following standards. [Total evaluation] ・"A": The average score of all 3 sensory evaluation items verified is 2.5 or above. ・"B": Does not match "A" and "C". ・"C": The average score of any of the three verified sensory evaluation items does not reach 2.0.

From the results of the examples, if the proline content in the beer-flavored beverage is 15~75mg/100mL, the total polyphenol content is 60~200 mass ppm, and the pyroglutamic acid content is 50~300mg/L, the apparent fermentation degree If it is 70.0~95.0%, it can provide a drink that suppresses "the satiety that is not suitable for beer-flavored drinks" and "the weak taste that is not suitable for beer-flavored drinks" and has "the rich taste that is suitable for beer-flavored drinks".

Claims (12)

A beer-flavored beverage characterized by a proline content of 15 to 75 mg/100 mL, a total polyphenol content of 60 to 200 mass ppm, a pyroglutamic acid content of 50 to 300 mg/L, and an apparent degree of fermentation of 70.0 to 95.0%. For example, for the beer-flavored beverage of claim 1, the ratio of the total polyphenol content (X) (unit: mass ppm) to the proline content (Y) (unit: mg/100mL) [(X)/(Y)] It is 1.5~5.5. For example, the beer-flavored beverage of claim 1 or 2, wherein the original extract concentration is 6.0~20.0% by mass. Such as requesting any one of the beer-flavored beverages 1 to 3, in which the ratio of the content of pyroglutamic acid (Z) (unit: mg/L) to the content of proline (Y) (unit: mg/100mL) [(Z)/(Y)] is 1.0~7.5. Such as the beer flavored beverage according to any one of claims 1 to 4, wherein the aforementioned beer flavored beverage is a fermented beer flavored beverage. For example, if the beer-flavored beverage in any one of the requirements 1 to 5 is required, its color should be 5.0 to 20.0EBC. For example, the pH of any of the beer-flavored beverages in claim items 1 to 6 is 3.0 to 5.0. For example, the beer-flavored beverage according to any one of claims 1 to 7, wherein the content of lactic acid is 40 to 950 mass ppm. For example, the beer-flavored beverage according to any one of claims 1 to 8 has a phosphoric acid content of 40 to 800 ppm by mass. For example, the beer-flavored beverage according to any one of claims 1 to 9, which contains ingredients derived from hops. A method for manufacturing a beer-flavored beverage, which is characterized by a total polyphenol content of 60 to 200 ppm by mass, a proline content of 15 to 75 mg/100 mL, and a pyroglutamic acid content of 50~300mg/L, and the apparent fermentation degree is set as 70.0~95.0%. A method for improving the aroma of beer-flavored beverages, characterized by the beer having a total polyphenol content of 60 to 200 ppm by mass, a proline content of 15 to 75 mg/100 mL, and a pyroglutamic acid content of 50 to 300 mg/L. In flavored beverages, the apparent fermentation degree is adjusted so that the apparent fermentation degree becomes 70.0~95.0%.