TW202321460A - Method for determining sanitary treatment location - Google Patents

Abstract

The present invention provides a sanitary treatment location determining method for detecting amylase in the environment, and determining a location in which to perform sanitary treatment, on the basis of the detection result.

Description

Determination method of sanitation place

The present invention relates to a method for determining a sanitation treatment place, a method for determining a sanitation treatment condition, a method for determining a sanitation treatment method, a sanitation treatment method, a sanitation treatment kit, an estimation method, and a method for displaying the pollution status of a sanitation treatment candidate and the method of displaying the place of sanitation.

The living environment around us includes various equipment, facilities, devices, etc., and various treatments are carried out in consideration of their respective uses. For example, in commercial facilities, accommodation facilities, etc., cleaning, sterilization, etc. are performed to maintain a hygienic state. At this time, it is proposed to determine the degree of stains and the like, and to determine treatment conditions and the like according to the degree of stains and the like.

A cleaning method is disclosed in Japanese Patent Laid-Open No. 2015-206671, which has: a specific detection method, which is to detect a substance to be tested such as a virus; and a cleaning step, which is to clean the above-mentioned The tested substance is removed from the object space (technical solution 19).

Japanese Patent Application Laid-Open No. 2012-5455 discloses a method for assessing the hygienic state of a living environment, which includes the step of detecting whether bacteria of the genus Methylobacterium exist in the living environment.

Japanese Patent Application Laid-Open No. 2013-36189 discloses a method for determining the cleaning period of the toilet and a method for determining the cleaning range of the toilet. The method for determining the cleaning period of the toilet includes: setting process, which is to use a specific toilet stain detection sheet Laminated materials or specific toilet stain detection sheets are arranged in multiple places in the cleaned area of the toilet; the judgment process is to judge the degree of stains in the above plural places according to the discoloration state of the installed sheet; and period The decision process is to determine the cleaning period of the toilet according to the degree of stains judged.

When cleaning, bleaching, deodorizing, killing viruses, sterilizing, etc. in the environment, in most cases, chemicals corresponding to the treatment are used, but in various facilities, especially commercial facilities, accommodation facilities, manufacturing equipment, etc. In facilities where the agent is applied to all premises, it is less efficient. That is, in such a large-scale facility, if it is possible to efficiently determine the place and time for cleaning and other treatments, labor and costs can be significantly reduced. In particular, in facilities used by unspecified people, it is important to prevent infection caused by viruses or bacteria, and thus an effective method for performing treatment reliably and efficiently is desired.

The present invention provides a method for determining sanitation treatment, which can simply and reliably determine the place or condition where sanitation treatment such as cleaning, disinfection, virus killing, and sterilization can be effectively performed.

The present invention relates to a method for determining a sanitation treatment place. It detects amylase in the environment and determines the sanitation treatment place based on the detection result.

In addition, the present invention relates to a method for determining sanitation treatment conditions, which is to detect amylase in the environment, and determine sanitation treatment conditions based on the detection results.

In addition, the present invention relates to a method for determining sanitation treatment method, which is to detect amylase in the environment, and determine the place where sanitation treatment is performed and the conditions of sanitation treatment based on the detection results.

Also, the present invention relates to a sanitizing method for determining at least any one of a place where sanitizing is performed and conditions for sanitizing by the determination method of the present invention, and sanitizing is performed based on the determination.

In addition, the present invention relates to a sanitation treatment kit, which includes: a mechanism for detecting amylase in the environment, and treatment of a sanitation place determined according to the detection result of amylase obtained by the mechanism medicine.

In addition, the present invention relates to an estimation method, which is to detect amylase in the environment, and estimate the place with high possibility of harmful virus and/or harmful bacteria based on the detection result.

Also, the present invention relates to an estimation method, which is to detect amylase in the environment, and based on the detection results, it is estimated that there is a high possibility of harmful viruses and/or harmful bacteria, and the route of infection is estimated.

In addition, the present invention relates to a method for displaying the pollution status of a candidate place for sanitation, which comprises the following steps: (a) The step of specifying one or more places in the environment, which includes the following steps (i) to (iii): (i) The step of generating location data that can identify the location where the physical object is installed, so as to identify the above-mentioned one or more locations within the defined area; (ii) the steps of recording the location data of the above-mentioned one or more premises in the above-mentioned environment; and (iii) The step of distinguishing and recording the location and location of the physical objects associated with the location data of the above-mentioned one or more premises in the above-mentioned environment; (b) the step of recording the test results of amylase detected from one or more places in the above-mentioned environment; (c) The step of recording the detection results generated according to the distinguished position and part of the physical object recorded in (iii) of (a) above; and (d) A step of displaying the record in (c) together with the location data of the above-mentioned one or more premises in the above-mentioned environment.

Also, the present invention relates to a display method of a sanitation treatment place, which determines the sanitation treatment place based on the pollution status of the sanitation treatment candidate places displayed by the above-mentioned display method of the present invention, and displays it as a sanitation treatment place .

According to the present invention, there is provided a method for determining a sanitizing place, a sanitizing condition, or a sanitizing method, which can simply and reliably determine a place where sanitizing such as cleaning, disinfection, virus elimination, and sterilization can be effectively performed. Also, according to the present invention, there is provided a method capable of easily and reliably estimating the location or infection route of harmful viruses and/or harmful bacteria.

In this specification, "sanitary treatment" can be the treatment of the environment for the purpose of maintaining health. For example, it refers to: removing "pollutants" from the "environment" and then "objects"; inactivating "pollutants" ; or both. In this specification, "pollutant" can be a substance that is harmful to health when present in an object, such as saliva, droplets from the oral cavity, and the like. Droplets from the oral cavity may contain saliva and amylase and other substances attached to saliva. Furthermore, the above-mentioned "substances attached to saliva" may include viruses and/or bacteria (sometimes referred to simply as "bacteria"), and may further be harmful viruses and/or harmful bacteria. In this specification, "pollution" can be, for example, the following situation: from the "environment" and further to the "object", there are no pollutants, such as saliva, and amylase and other substances attached to saliva, which become unfavorable. The presence of pollutants, such as saliva, and amylase and other substances attached to saliva in healthy amounts.

Amylase is an enzyme contained in human saliva. The present invention found that: the detection degree of amylase attached to the surface of the living environment is related to the possibility of contamination caused by components containing amylase, and found that it is judged by the higher detection degree of amylase As a place with a high possibility of pollution, it is determined that the place is a place where cleaning, disinfection, virus killing, sterilization and other sanitation treatments are carried out, which can efficiently prevent and remove pollution caused by viruses and/or bacteria. Furthermore, the present invention finds that the possibility of the existence of harmful viruses and/or harmful bacteria or the infection route can be estimated with high accuracy based on the detection degree of amylase. Previously, the possibility of contamination was not determined based on the detection level of amylase, and the place to implement sanitation treatment was determined; and the possibility of the existence of harmful viruses and/or harmful bacteria was not estimated based on the detection level of amylase, and then presumed infection path.

In the present invention, the detection of amylase in the environment is carried out. The environment may be a living environment, a natural environment, or the like. The environment, especially the living environment, may include buildings, public transport. In addition, buildings and public transportation may include their accompanying equipment. There are no restrictions on the scale and use of the building.

The building may, for example, be a building selected from commercial facilities, accommodation facilities, educational facilities, medical facilities, nursing facilities, business facilities, and dining facilities, or complex facilities thereof. As a commercial facility, a shopping center, a supermarket, a movie theater, an amusement park, etc. are mentioned, for example. As the accommodation facility, for example, a hostel, an inn, a bed and breakfast, etc. may be mentioned. As an educational facility, various schools, a cultural center, etc. are mentioned, for example. As a medical facility, a hospital is mentioned, for example. As a care facility, a nursing home (carehouse) for the elderly etc. are mentioned, for example. As a management facility, an office building, a factory, etc. are mentioned, for example. As a dining facility, a restaurant, a teahouse, a fast food restaurant, etc. are mentioned, for example.

The public transportation means may, for example, be a public transportation means selected from rail vehicles, road vehicles, ships, and airplanes. As a rail vehicle, an electric car, a diesel car, etc. are mentioned, for example. The road vehicle may, for example, be a bus or the like. The ship may, for example, be a passenger ship or the like. As an airplane, a passenger plane etc. are mentioned.

In the present invention, the environment includes an object to be detected for amylase (hereinafter referred to as an object to be detected), and amylase on the surface of the object to be detected can be detected. The detection object may include, for example, a detection object selected from buildings, building equipment, public transportation, and public transportation equipment. In the present invention, it is possible to detect amylase on the surface of an object to be detected, for example, an object disposed indoors. Items can be either fixed items or unfixed items. The material of the article is also not limited. Examples of articles include: furniture, electrical appliances, articles requiring water, building materials, building tools, clothing, bedding, etc. More specifically, examples include: tables, chairs, kitchen sinks, toilet seats, Items in door handles, faucets, switches, remote operating machines (remote controls for various machines, etc.), input devices for computers (keyboards, mice, etc.), wallpapers, fabric ceilings, curtains, and carpets. In addition, as articles, for example, switches of elevators installed in buildings, handrails of escalators installed in buildings, automatic ticket vending machines of vehicles, rings of vehicles, shopping baskets frequently used in commercial facilities, or Items such as carts. In the present invention, it is possible to detect amylase on the surface of the articles as detection objects.

In the present invention, the environment may be an area to be subjected to sanitation (hereinafter also referred to as an object area). Hereinafter, the term of the target area may refer to the environment. The target area can be selected from, for example, buildings such as commercial facilities, accommodation facilities, and business facilities described above, public transportation, and the like. A location for amylase detection is selected from the target areas. The place where amylase is detected can be appropriately selected in consideration of the use, scale, and the like of the target area. In the present invention, it is possible to specify an area to be subjected to sanitation in the environment, and to detect amylase in the area to be treated. That is, in the present invention, it is possible to specify an area to be subjected to sanitation in the environment, and to detect amylase in the object to be detected in the area.

In the present invention, it is preferable to detect the amylase on the surface of the object to be detected in the region to be detected, and to measure the amount of amylase if necessary. Furthermore, the amylase may be amylase from human.

Samples for detecting amylase from the surface of the object to be detected in the target area can be collected by bringing an appropriate collection mechanism such as absorbent cotton, non-woven cloth, sponge, etc. into contact with the surface of the object to be detected. In the present invention, when amylase is detected from the surface of the object to be detected, it is preferable to use a collection mechanism having a support and a sample collection section provided on the support. In the present invention, it is suitable to use a swab or a cotton swab as the collecting means. These can be used for medical treatment or sample collection. Various shapes, such as a spherical shape and a vertical shape, can be used for the said collection part. In addition, the above-mentioned collecting part may contain, for example, fibers, porous materials, and the like. Specific examples of the fibers include natural fibers such as cotton, synthetic fibers, and blended fibers of these. A sponge etc. are mentioned as a porous material. The collecting part is preferably liquid-absorbent. It is preferable that the above-mentioned collecting part is brought into a wet state with an appropriate liquid. That is, it is preferable to carry the liquid on the collection part. The liquid carrying capacity in the collecting part is preferably such that dripping does not occur. The dry mass of the collecting part, such as a fibrous material or a porous material, is preferably not less than 10 mg and not more than 1000 mg, for example. The above-mentioned liquid used to be carried on the collection part may be, for example, water. In addition, the above-mentioned liquid may be an aqueous solution containing a surfactant, physiological saline, buffer solution, etc., and may be a composition containing components prepared in these. The surfactant can be selected from those that will not affect the detection of amylase. As the surfactant, for example, a nonionic surfactant can be exemplified. As the nonionic surfactant, for example, an alkyl group having a carbon number of 6 to 22, and an average added mole number of oxypropylidene group are Polyoxyalkylene alkyl ethers and polyoxyethylene alkylphenyl ethers, polyoxyethylene alkylphenyl ethers, polyols such as glycerin, pentaerythritol, and sorbitol, and polyols such as glycerin, pentaerythritol, and sorbitol, and polyoxyalkylene alkyl ethers and polyoxyethylene alkylphenyl ethers with an average addition mole number of oxyethylidene groups of 5 to 50. Polyoxyalkylene adducts of esters of fatty acids, etc. As the nonionic surfactant, for example, those sold under trade names such as Triton X-100, Nonidet P-40 (NP-40), Tween20, and Tween80 can be used. The concentration of the surfactant in the liquid can be determined within the range that does not affect the detection of amylase. The concentration of the surfactant in the liquid may be, for example, 2 mass % or less, further may be 1 mass % or less, further may be 0.5 mass % or less, further may be 0.1 mass % or less, further may be 0.05 mass % or less, and further may be It may be 0.01 mass % or less, and further may be 0.005 mass % or less.

In order to obtain common information by unifying the collection conditions in the sample collection place, such as a specific site unit, a room unit, a building unit, or an area unit, it is preferable to use a system that allows the same type of collection unit to carry approximately the same amount of liquid. collection agency. That is, since a plurality of collecting mechanisms are usually used when collecting a sample, it is preferable to have the same type of collecting parts, and to carry the same amount of liquid in the collecting parts at the initial stage before contacting the surface of the object to be tested. Therefore, it is also possible to prepare a plurality of supports in which the collection part is wetted with a predetermined amount of liquid in an aluminum package or a container with high airtightness. In addition, in the case of separating the collection part from the incipient wetting liquid, it is also conceivable to put a fixed amount of liquid in a microtube or a test tube, seal it, open it before use, and bring it into contact with the collection part. The collecting part is made to absorb liquid, thereby preventing water from being absorbed beyond a certain amount. Regarding the amount of the liquid carried by the collecting part, in the case of a cotton swab, etc., it is preferably at least 10 mg, more preferably at least 30 mg, further preferably at least 90 mg, and more preferably not more than 1500 mg and not more than 1000 mg , less than 500 mg. The amount of liquid absorbed can vary according to the inspection area or the nature of the collection part, but it is preferably less than 200 mg, more preferably 100 mg, and it is usually easy to use. The ratio of the liquid to the collecting part (for example, the cotton of a cotton swab) is preferably 0.5 or more, more preferably 1 or more, further preferably 2 or more, further preferably 3 or more, and more preferably 5 or less in terms of mass ratio , more preferably 4 or less.

Moreover, as said support body, concretely, a thing made from wood and resin can be mentioned. When the amylase is not immediately extracted from the collected cotton swab, for example, when it is brought back to a laboratory different from the collection site for extraction, since the extraction of amylase will become difficult, the support is preferably made of resin. By. The reason for this is considered to be that in the case of wood, the liquid gradually migrates from the collecting part to the supporting part. Also, when the amylase is not immediately extracted from the collected cotton swab, it is preferable to store the collecting mechanism such as a cotton swab at a temperature of preferably below 10°C, more preferably below 5°C, and preferably above 0°C, more preferably Store at a temperature above 2°C. It is preferable to carry out transportation, storage, etc. of the collection mechanism within this temperature range. At this time, it is better to seal the cotton swab in a plastic container with a sealing strip or a sample bottle, an aluminum package, or a container with higher airtightness for storage. In addition, when the amylase is not detected immediately from the extract collected from the collection part, it is also preferably at a low temperature, preferably below 10°C, more preferably below 5°C, and preferably above 0°C, More preferably, it is stored at a temperature above 2°C. It is preferable to carry out transportation, storage, etc. of the extract within this temperature range.

In addition, in the case of using the above-mentioned collection mechanism, with regard to the area to be inspected by one collection unit, when the liquid contained in the collection unit is 90 mg to 500 mg, one collection unit shall be subject to inspection The area is preferably at least 100 mm ² , more preferably at least 2,000 mm ² , and from the viewpoint of maintaining the wetness of the collecting part and maintaining the wiping efficiency of amylase, it is preferably at most 30,000 mm ² , more preferably at least 10,000 mm ² the following. Regarding the sample collection method using the above-mentioned collection mechanism, in the case of a wide inspection area, it is better to assume a square with an area equivalent to the above-mentioned area as the target surface, and in this surface, in the vertical, horizontal, and oblique directions (2 In each direction of the diagonal line), the number of contacts is determined within the range of 10 to 20 times and collected, that is, in one surface, the total number of contacts in one direction × 4 is collected. . When the inspection surface is curved or rectangular, it is sufficient to set a range close to the determined area to collect samples.

In the case of using the above collection mechanism, extraction of amylase from the above collection part after contact with the surface of the detection object can be carried out by bringing the collection part into contact with the extraction liquid, for example, making the collection part Immerse in the extract contained in a suitable container. The amount of the extract also varies depending on the size of the collecting part. For example, in the case of a cotton swab, the amount of the extract is preferably at least 30 mg, more preferably at least 50 mg, and more preferably at most 10,000 mg. It is 5000 mg or less, more preferably 1000 mg or less. Preferably, the amount of the extract is 0.1 times or more, further 0.5 times or more, and 10 times or less, further 5 times or less, and furthermore 2 times or less the amount of the liquid impregnated in the collection part on a mass basis . In the case of immersion, the immersion time may be, for example, 10 seconds or more, further 1 minute or more, 5 minutes or more, 10 minutes or more, 30 minutes or more, 1 hour or more, 2 hours or more, and less than 48 hours, and further less than 48 hours. 24 hours, less than 12 hours, less than 6 hours. In the case of immersion, the immersion temperature is a temperature at which amylase does not undergo modification, for example, immersion can be performed at room temperature (for example, 10° C. to 30° C.). As the extract liquid, those having the same composition as the above-mentioned liquids can be used. The collection part can be left still while being immersed in the extraction solution, or it can be loaded with external force, such as the application of ultrasonic waves, stirring with a stirring device such as a vortex mixer, manual shaking, and squeezing with a rod, etc. , Extrusion against the wall of the container, etc. Also, when using a small amount of extract, the liquid can be separated from the collection part by centrifugation, pressurization, or the like. For example, amylase may be detected from the extract obtained by contacting the collection part with the above-mentioned method by the following method using antigen-antibody reaction and/or enzyme reaction. Furthermore, a suitable commercially available kit can be used for collection and extraction of amylase, and in this case, collection of amylase can also be performed according to the method described in the kit. In addition, the above-mentioned liquid and the above-mentioned extraction liquid and the like can also be used in the attached one of the kit.

As an aspect of the present invention, a method for determining a sanitation place can be exemplified, which is to detect amylase on the surface of the object to be detected in the environment, and furthermore, the surface of the object to be detected in the living environment, and based on the detection results to determine Decide where to sanitize. As another aspect of the present invention, a method for determining a sanitary treatment place can be exemplified, which is to detect amylase on the surface of the detection object in the environment, and then the surface of the detection object in the living environment, and based on the detection result The method to determine the place for sanitation treatment, and it is to use a collection mechanism with a support part and a sample collection part set on the support body, preferably a swab and/or a cotton swab, to collect from the surface of the above-mentioned detection object samples, and amylase assays were performed from the samples.

In the present invention, the detection of amylase can be performed on different places in the target area, and the places where the detection amount of amylase is relatively large in the tested places are set as the places where sanitation treatment is performed.

In the present invention, the detection of amylase in the target area is carried out, and the conditions of sanitation treatment are determined based on the detection results, such as the type of treatment, the type or amount of medicament used in the treatment, the number of times of treatment, etc. As for the conditions, the conditions usually adopted by the business operators for each treatment can be adopted as they are, or can be adopted by modifying them.

It is assumed that amylase or a component containing amylase, such as saliva, tends to adhere in various forms where a large amount of amylase is detected. It is assumed that such a place is a place where contamination by, for example, saliva or viruses and/or bacteria entering into saliva is likely to regenerate even if it is cleaned temporarily. It can be taken into consideration when determining the location or conditions of sanitation based on the results of the amylase test.

In the present invention, the detection of amylase can be carried out at different places in the target area, and the conditions of sanitation treatment can be determined according to the detection frequency and amount of amylase. Specifically, the detection of amylase can be carried out at different places in the target area, and the conditions of sanitation treatment can be determined according to the positive rate of amylase and the average amount of amylase calculated from the detection results. Here, the positive rate of amylase is the ratio of the number of places where amylase is detected to the number of places where amylase has been detected, and can be calculated by the following formula. Amylase positive rate (%) = B/A × 100 A: Number of locations where amylase testing was performed B: Number of locations where amylase was detected In addition, the average amount of amylase is the average value of the amount of amylase in the place where the amylase test was carried out. In this case, the amount of amylase may be a result indicating a relatively detected amount, for example, based on the detection strength of a reagent.

It can be explained that the positive rate of amylase is the probability that the target area is polluted, and the average amount of amylase is the amount of pollutants attached to the target area on average. In the present invention, the possibility of contamination caused by viruses and/or bacteria can be determined based on the detection results of amylase. In this case, the greater the amylase positive rate and the average amylase amount, the higher the risk of infection when a person comes into contact with the surface of the subject area. In the present invention, it is preferable to set a criterion for determining the possibility of contamination by viruses and/or bacteria based on the positive rate of amylase and the average amount of amylase, and determine the risk of infection based on the criterion. Specifically, for the positive rate of amylase and the average amount of amylase, a criterion of more than 2 stages, preferably more than 3 stages, and less than 10 stages, preferably less than 5 stages can be set for the determination. Infection risk, judging the priority of sanitation, etc. For example, as shown in the following Table 1, the positive rate of amylase can be correlated with the average amount of amylase to determine the risk and determine the priority of sanitation. Furthermore, the risk judgment criteria in Table 1 are an example, and the positive rate of amylase and the impact of the average amount of amylase on the infection risk can be judged according to different criteria from Table 1.

In the present invention, when amylase is detected at different places in the target area, for example, more than 1 place, further more than 2 places, further more than 3 places, And measurement is performed at a plurality of locations selected at a ratio of 16 locations or less, further 9 locations or less, and further 6 locations or less. The above-mentioned different locations can be selected in the same detection object, or can be selected in different detection objects.

In the present invention, amylase can be detected by using antigen-antibody reaction and/or enzyme reaction. The detection of amylase in the target area may be performed directly on the surface of the detection target, for example, or may be performed on a sample collected from the surface of the detection target. In the present invention, it is preferable to detect amylase by using the method of antigen-antibody reaction. In the present invention, for example, it is preferable to detect amylase by a method using an antigen-antibody reaction capable of specifically detecting human salivary amylase. Examples of methods utilizing antigen-antibody reactions include: immunochromatography, enzyme immunoassay (EIA (Enzyme Immunoassay, enzyme immunoassay) method, ELISA (Enzyme-linked immunosorbent assay, enzyme-linked immunosorbent assay) method), Immunoprecipitation, immunoblotting, etc. Commercially available products that measure amylase levels using antigen-antibody reactions can also be used.

In the present invention, sanitation treatment can be a treatment performed on the target area that has undergone amylase detection. That is, in the present invention, for example, when amylase is detected on the surface of the object to be detected, the sanitation treatment may be performed on the object to be detected and/or the environment including the object to be detected.

In the present invention, sanitation treatment may include treatment to improve the sanitation state of the target area, treatment to maintain the sanitation state of the target area, and the like. In general, the hygienic state of the living environment is improved and/or maintained by keeping it clean. In the present invention, for example, the sanitation treatment may include one or more treatments selected from cleaning, antibacterial adhesion prevention treatment, and virus adhesion prevention treatment. More specifically, the sanitation treatment may include one or more treatments selected from, for example, washing, bleaching, disinfection, sterilization, sterilization, antibacterial, virus killing, antiviral, and virus removal. Each treatment can be performed by a known method.

In the present invention, sanitation treatment can be a treatment for reducing adverse effects caused by viruses and/or bacteria. That is, the viruses and/or bacteria targeted by the present invention may be harmful viruses and/or harmful bacteria. Harmful at least includes harmful to people. Harmful viruses may be non-enveloped or enveloped viruses, in particular may be enveloped viruses. Regarding the treatment for reducing adverse effects caused by viruses and/or bacteria, specifically, it can be selected from the above-mentioned treatments. Adverse events caused by viruses and/or bacteria are typically infections.

In the present invention, sanitation treatment may be treatment using a sanitation treatment agent (hereinafter referred to as a sanitation treatment agent) for at least any one selected from cleaning, sterilization, and virus killing. The sanitizing agent may contain, for example, one or more selected from (a) surfactants, (b) oxidizing bleaches, and (c) bactericides. The sanitizer may contain water. The sanitizing agent can be any of liquid and solid, preferably liquid, more preferably liquid containing water.

As (a) surfactant, one or more types selected from anionic surfactants, nonionic surfactants, cationic surfactants, and amphoteric surfactants are mentioned. As (a) the surfactant, for example: selected from linear alkylbenzene sulfonic acid and its salt, alkyl sulfate and its salt, polyoxyethylene alkyl ether sulfuric acid and its salt, fatty acid and its salt, One or more of polyoxyethylene alkyl ethers, alkyl glycosides, alkylamine oxides, quaternary ammonium salts, and alkylamine ethylene oxide adducts. The carbon number of the linear alkyl group of the linear alkylbenzene sulfonic acid may be 8 or more and 20 or less. The carbon number of the alkyl group of the alkyl sulfate may be 8 or more and 20 or less. The average addition mole number of ethylene oxide in polyoxyethylene alkyl ether sulfuric acid can be from 1 to 30, and the carbon number of the alkyl group can be from 8 to 20. The carbon number of the fatty acid may be 8 or more and 20 or less. As a salt, alkali metal salts, such as a sodium salt and a potassium salt, are mentioned. The average added mole number of ethylene oxide in the polyoxyethylene alkyl ether can be 1 to 30, and the carbon number of the alkyl group can be 8 to 20. The carbon number of the alkyl group of the alkyl glycoside may be 8 or more and 20 or less. The carbon number of the alkyl group of the alkylamine oxide may be 8 or more and 20 or less. As the quaternary ammonium salt, benzalkonium chloride, benzethonium chloride, dialkyl (carbon number 8 or more and 20 or less) dimethyl ammonium chloride, etc. may be mentioned. The carbon number of the alkyl group of the alkylamine ethylene oxide adduct can be from 8 to 20, the average addition mole number of ethylene oxide can be from 1 to 30, and the amine can be primary, secondary, or tertiary any of the levels. As an alkylamine ethylene oxide adduct, a laurylamine ethylene oxide adduct is mentioned, for example. Although it also depends on the type of sanitizing treatment, the sanitizing agent may contain, for example, 0.0001 mass % or more, further 0.001 mass % or more, further 0.01 mass % or more, further 0.1 mass % or more, and 20 mass % or less, and further The (a) surfactant is 10% by mass or less, further 5% by mass or less.

(b) Oxidative bleaching agent may, for example, be hypochlorite, dichloroisocyanurate, hydrogen peroxide, sodium percarbonate or peracetic acid, preferably hypochlorite. As a salt, alkali metal salts, such as a sodium salt and a potassium salt, are mentioned. Although it also depends on the type of sanitation treatment, the sanitation treatment agent may contain, for example, 0.0001 mass % or more, further 0.001 mass % or more, further 0.01 mass % or more, further 0.1 mass % or more, further 1 mass % or more, and (b) Oxidative bleaching agent of 10 mass % or less, further 7.5 mass % or less, further 5 mass % or less.

As (c) a bactericide, one or more types selected from a bactericidal alcohol and a bactericidal organic acid are mentioned.

As a sterilizing alcohol, one or more types selected from ethanol and propanol may be mentioned. Although it also depends on the type of sanitizing treatment, the sanitizing agent may contain, for example, 0.1 mass % or more, further 1 mass % or more, further 10 mass % or more, further 40 mass % or more, and 95 mass % or less, and further 90% by mass or less, further 85% by mass or less of fungicidal alcohol.

Lactic acid or its salt is mentioned as a bactericidal organic acid. As a salt, alkali metal salts, such as a sodium salt and a potassium salt, are mentioned. Although it also depends on the type of sanitation treatment, the sanitation treatment agent may contain, for example, 0.01 mass % or more, further 0.1 mass % or more, further 0.4 mass % or more, and 7 mass % or less, further 5 mass % or less, and further A bactericidal organic acid of 3% by mass or less.

(c) The fungicide may be one or more selected from ethanol, propanol, lactic acid, and a salt of lactic acid.

It is preferable that a sanitizing agent contains 2 or more types chosen from (a) surfactant, (b) oxidative bleaching agent, and (c) bactericide.

In the present invention, the sanitation treatment may include: bringing the sanitation agent into contact with the treatment place, and wiping the treatment place in contact with the sanitation treatment agent with a flexible liquid-absorbing material. In the present invention, the sanitizing agent can be brought into contact with the treatment place by at least one of the following methods: making a flexible liquid-absorbent material impregnated with the above-mentioned sanitizing agent contact the treatment place, and spraying the above-mentioned sanitizing agent onto processing premises. For example, if the place to be treated is wiped with a flexible liquid-absorbing material impregnated with a sanitizing agent, the place to be treated can be wiped while the sanitizing agent is brought into contact with the place to be treated. Spraying can be performed using, for example, a trigger pump or the like. Examples of the flexible liquid-absorbent material include non-woven fabrics, woven fabrics, and sponges.

According to the present invention, a method for determining sanitation treatment method is provided, which is to detect amylase in the environment, and determine the place where sanitation treatment is performed and the conditions of sanitation treatment based on the detection result. In this method, the method of determining the sanitation place can be the method of determining the sanitation place of the present invention. In addition, in this method, the method of determining the conditions of sanitation treatment may be the method of determination of sanitation treatment conditions of the present invention. The method of sanitation shall be determined according to the location and conditions of sanitation determined in this way.

In the present invention, the detection of amylase is implemented in order to determine the place and/or conditions for sanitization. In the present invention, the detection of amylase can be carried out every time sanitation is performed, but it is not necessary to carry out every time. Also, for example, if based on the information of the location and/or conditions determined by the present invention, the type, degree, frequency, and other treatment methods of sanitation for each location are manualized, then as long as the use of the location does not occur Larger changes, even if the amylase test may not be carried out, the highly hygienic treatment can still be implemented continuously. In addition, once the place and/or conditions for sanitation are determined, the detection of amylase can be carried out regularly or irregularly, and the quality and effect of the determined sanitation method can also be confirmed.

The determination method of the present invention can be used to support sanitation treatment, for example, for the above-mentioned buildings, public transport vehicles, and equipment thereof. That is, information on the sanitization method determined by the present invention can be provided to the performer of sanitation, so that they can perform specific sanitation reliably and efficiently.

As a more specific aspect of the present invention, a method of determining a sanitation treatment method can be exemplified, which is to detect amylase in the target area, and determine the place where the amylase is detected as possible presence of viruses and/or bacteria Based on the results of the amylase test, determine the conditions for the sanitation treatment of the above-mentioned places. In this method, the matters described in the above-mentioned determination methods of the present invention can be appropriately applied. As another more specific aspect of the present invention, a method for determining a sanitation treatment method can be exemplified, which is to detect amylase in buildings, such as indoors and/or outdoors, and determine the place where amylase is detected as There may be places contaminated by viruses and/or bacteria, and based on the results of the amylase test, determine the conditions for the sanitation of the above-mentioned places. In this method, the matters described in the above-mentioned determination methods of the present invention can be appropriately applied.

According to the present invention, there is provided a method of sanitation, which is to determine at least any one of the place where sanitation is performed and the conditions of sanitation by the above-mentioned determination method of the present invention, and perform sanitation based on the determination. That is, the sanitary treatment method of the present invention is the following sanitary treatment method: (1) Use the determination method of the present invention to determine the place where sanitation is to be performed, and perform sanitation at the determined place; (2) Use the determination method of the present invention to determine the conditions of sanitation treatment, and perform sanitation treatment under the determined conditions; or (3) Use the determination method of the present invention to determine the place where sanitation treatment is performed and the conditions for sanitation treatment, and perform sanitation treatment at the determined place under the determined conditions. In the sanitization method of the present invention, the matters described in the determination method of the present invention can be appropriately applied. In addition, the sanitization method of the present invention is also preferably sanitization using a sanitizing agent. Specific examples or preferred examples of sanitation treatment and sanitation treatment agent can also be applied to those described in the determination method of the present invention.

According to the present invention, there is provided an estimation method of detecting amylase in the environment and estimating, based on the detection results, places with a high possibility of harmful viruses and/or harmful bacteria. Also, according to the present invention, an estimation method is provided, which is to detect amylase in the environment, and based on the detection results, it is estimated that there is a high possibility of harmful viruses and/or harmful bacteria, and the route of infection is estimated, that is, the The infection path of harmful viruses and/or harmful bacteria. The route of infection here may be the route of infection to humans. Also, the route of infection may be any of the route of infection that has already occurred and the route of infection predicted from this. Furthermore, the estimation method mentioned in the present invention also includes: in the initial environment, with the accumulation of historical data, the possibility of the existence of harmful viruses and/or harmful bacteria is estimated through empirical insights or artificial intelligence (AI), etc. Higher places or routes of infection. In addition, the detection of amylase can also be performed after estimation. In these methods, the matters described in the above-mentioned respective determination methods of the present invention can be appropriately applied. Also, in these methods, the harmful virus can likewise be a non-enveloped or enveloped virus, especially an enveloped virus.

Examples of harmful bacteria include Streptococcus pneumoniae and the like. Moreover, examples of non-enveloped viruses include adenovirus, rhinovirus, and norovirus. Also, as enveloped viruses, for example, SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2, Severe Acute Respiratory Syndrome Coronavirus 2), influenza, SARS (Severe Acute Respiratory Syndrome, Severe Acute Respiratory Syndrome ), MERS (Middle East Respiratory Syndrome, Middle East Respiratory Syndrome) and RS (Respiratory syncytial, respiratory fusion) virus, etc.

According to the present invention, there is provided a sanitation kit, which includes: a mechanism for detecting amylase in an object area, and a sanitation place for treatment based on the detection result of amylase obtained by the mechanism medicine. Examples of means for measuring the amount of amylase include immunochromatography, enzyme immunoassay (EIA, ELISA), immunoprecipitation, immunoblotting, and enzyme activity measurement. Moreover, as said chemical|medical agent, a cleaning agent, a bleaching agent, a disinfectant, a viricide, an antiviral agent, a bactericide, an antibacterial agent, etc. are mentioned, for example.

According to the present invention, there is provided a method for displaying the pollution status in a candidate place for sanitation, which comprises the following steps: (a) The step of specifying one or more places in the environment, which includes the following steps (i) to (iii): (i) The step of generating location data that can identify the location where the physical object is installed, so as to identify the above-mentioned one or more locations within the defined area; (ii) the steps of recording the location data of the above-mentioned one or more premises in the above-mentioned environment; and (iii) The step of distinguishing and recording the location and location of the physical objects associated with the location data of the above-mentioned one or more premises in the above-mentioned environment; (b) the step of recording the test results of amylase detected from one or more places in the above-mentioned environment; (c) The step of recording the detection results generated according to the distinguished position and part of the physical object recorded in (iii) of (a) above; and (d) A step of displaying the record of (c) together with the location data of the above-mentioned one or more premises in the above-mentioned environment. The method for displaying the pollution status of the present invention is preferably performed by an information processing device (PC (Personal Computer, personal computer), smart phone, etc.). That is, the recording of each data in each step is preferably performed by an information processing device. FIG. 1 shows the outline of the steps of the method of displaying the pollution status of the candidate places for sanitation according to the present invention. In Fig. 1, ST is the abbreviation of step. In FIG. 1 , step (a) not shown is executed by ST(a)-(i), ST(a)-(ii), and ST(a)-(iii).

Step (a) is the step of specifying one or more places in the environment, and appropriately select the places in the environment as candidates for sanitation. For example, as the above-mentioned place, items in the room, and further items in the toilet can be selected. Step (i) [ST(a)-(i) in FIG. 1] is a step of generating positional data that can identify the location where the physical object is installed, so as to specify the above-mentioned one or multiple locations within the defined area. The aforementioned area can be, for example, a specific area indoors. As the above-mentioned physical objects, for example, items in a room, and further items in a toilet can be mentioned. Location data is, for example, data indicating the location of the items indoors. Step (ii) [ST(a)-(ii) in FIG. 1] is a step of recording the location data of the above-mentioned one or multiple premises in the above-mentioned environment. Step (iii) [ST(a)-(iii) in FIG. 1] is a step of distinguishing and recording the positions and parts of the entities associated with the position data of the above-mentioned one or a plurality of premises in the above-mentioned environment. For example, when there are a plurality of items of the same attribute in a room, they are differentiated and information is created and recorded separately. Specifically, for example, when there are two chairs in a room, they are assigned as chair A and chair B, and their respective position data are recorded. Furthermore, when each chair has two armrests, the positional data can be created by subdividing the armrest A1 and the armrest A2. Of course, this processing method can be applied to other physical objects (articles, etc.).

Step (b) [ST(b) in FIG. 1] is a step of recording the detection results of amylase detected from one or more places in the above environment. The detection of amylase is carried out in the position and location of the entity distinguished by step (iii) of step (a). The detection result can be obtained, for example, by the detection method adopted in the method for determining the sanitary treatment place of the present invention.

Step (c) [ST(c) in Fig. 1] is to record by associating the generated detection results with the distinguished positions and parts of the physical objects recorded in step (iii) of step (a) A step of. Through the step (c), the detection results of the physical object at the specific position and the detection results of the specific part of the physical object at the specific position are recorded, so that the position and position of the physical object are associated with the detection results.

Step (d) [ST(d) in FIG. 1] is a step of displaying the record of step (c) together with the location data of the above-mentioned one or multiple places in the above-mentioned environment. The display is performed, for example, by an information display terminal such as an external monitor for a computer or a screen of a smartphone.

For example, in Figure 1, ST(a)-(i)～ST(d) is set as one unit, and ST(a)-(i)～ST(d) is repeated until a predetermined number of times is reached. It can also end after reaching a certain number of times. Also, for example, in FIG. 1, ST(a)-(i)-ST(d) is set as one unit, and ST(a)-(i)-ST(d) is repeated at predetermined intervals. , and can also end after reaching a specific time.

According to the present invention, there is provided a method of displaying a sanitation place, which determines the sanitation place based on the pollution status of the sanitation candidate places displayed by the above-mentioned display method of the present invention, and displays it as the sanitation place. The display method of the sanitary treatment place of the present invention is preferably performed by an information processing device (PC, smart phone, etc.). In the display method of sanitation treatment places of the present invention, based on the data of multiple sanitation treatment candidate places obtained by changing the measurement period for one or a plurality of places in the environment specified in the above step (a), Decide on sanitation premises. The decision of sanitation place can utilize AI, for example. That is, for example, data such as amylase test results, places and articles that have been tested for amylase, past infection occurrences, etc. can be accumulated, and AI can be used to determine sanitation places.

The method of displaying candidate places for sanitation treatment and/or the method of displaying sanitation treatment places of the present invention can be incorporated into the method of determining sanitation treatment places, the method of determining sanitation treatment conditions, the method of determining and estimating sanitation treatment methods of the present invention in the implementation. For example, in the display method of sanitation treatment place of the present invention, the possibility of contamination caused by viruses and/or bacteria can be judged based on the pollution state in the method of displaying the pollution state of the present invention, and the decision can be made according to the judgment result Hygienic treatment premises. The determination of the possibility of pollution may also be constituted by presuming the possibility of pollution. Furthermore, the results displayed by the present invention can be printed on a paper medium or the like for output if necessary. Also, the electronic data of the displayed results can be stored in a suitable storage medium. In addition, the information communication network (Internet) can be used to carry out secondary utilization of the electronic data of the displayed results, for example, it can be used in the production of the implementation plan of the sanitation treatment, the step guidance of the sanitation treatment based on the above-mentioned implementation plan and other services.

The aspect of this invention is illustrated below. Among these aspects, the method of determining a sanitation place, the method of determining a sanitation condition, the method of determining a sanitation method, the method of sanitation, a sanitation kit, an estimation method, and a candidate for sanitation can be appropriately applied. Matters described in the display method of the pollution status in the premises and the display method of the sanitary treatment premises.

<1> A method for determining a place for sanitation, which is to detect amylase in the environment, and determine a place for sanitation based on the detection result.

<2> The determination method as described in <1> is to test amylase in different places in the environment, and the place where the detected amount of amylase is relatively large in the tested place is set as the place where sanitation is performed.

<3> The method of determining the sanitation place as described in <1> or <2> is based on the amylase test results to determine the possibility of contamination caused by viruses and/or bacteria, and decides to perform sanitation based on the results of the determination place of processing.

<4> A method for determining sanitation treatment conditions is to detect amylase in the environment and determine sanitation treatment conditions based on the detection results.

<5> As described in <4>, the determination method is to detect amylase in different places in the environment, and determine the conditions of sanitation according to the detection frequency and amount of amylase.

<6> The determination method described in <4> or <5> is to determine the possibility of contamination caused by viruses and/or bacteria based on the detection results of amylase, and determine the conditions of sanitation according to the determination results.

<7> A method for determining sanitation treatment method, which is to detect amylase in the environment, and determine the place where sanitation treatment is performed and the conditions of sanitation treatment based on the detection results.

<8> The determination method as described in <7> is to test amylase in different places in the environment, and the place where the detected amount of amylase is relatively large in the tested place is set as the place where sanitation is performed.

<9> The determination method described in <7> or <8> is to detect amylase in different places in the environment, and determine the conditions of sanitation according to the detection frequency and amount of amylase.

<10> The determination method described in any one of <7> to <9> is to determine the possibility of contamination caused by viruses and/or bacteria based on the detection results of amylase, and to determine sanitation based on the results of the determination Conditions of premises and/or sanitization.

<11> The determination method described in any one of <1> to <10>, wherein the sanitation treatment is a treatment for reducing adverse effects caused by viruses and/or bacteria.

<12> The determination method described in any one of <1> to <10>, wherein the sanitation treatment includes one or more treatments selected from cleaning, antibacterial adhesion prevention treatment, and virus adhesion prevention treatment.

<13> The determination method described in any one of <1> to <12> is to detect amylase by utilizing antigen-antibody reaction and/or enzyme reaction.

<14> The determination method described in any one of <1> to <13>, which is to detect amylase by using the method of antigen-antibody reaction.

<15> The determination method according to any one of <1> to <14>, wherein the amylase is human-derived amylase.

<16> The determination method described in any one of <1> to <15>, wherein the environment is a living environment.

<17> The determination method described in any one of <1> to <16>, wherein the environment includes an object to be detected for amylase (hereinafter referred to as the object to be detected), and the amylase on the surface of the object to be detected is detected.

<18> The determination method described in <17>, wherein the detection object includes a detection object selected from buildings, building equipment, public transportation, and public transportation equipment.

<19> The determination method described in <18>, wherein the buildings are selected from commercial facilities, accommodation facilities, educational facilities, medical facilities, nursing facilities, business facilities, and catering facilities, and the public transportation means are selected from rail vehicles , road vehicles, ships, and public transport in aircraft.

<20> The determination method described in any one of <17> to <19>, wherein the detection object includes a table, a chair, a kitchen sink, a toilet seat, a doorknob, a faucet, a switch, a remote operation device, and a computer The detection object in the input device.

<21> The determination method described in any one of <1> to <20>, wherein the sanitizing treatment is a treatment using at least one sanitizing agent selected from the group consisting of washing, sterilizing and killing viruses.

<22> The determination method described in <21>, wherein the sanitizing agent contains one or more selected from the group consisting of (a) surfactant, (b) oxidative bleaching agent, and (c) bactericide.

<23> The determination method described in <21> or <22>, wherein the sanitizing agent contains two or more selected from (a) surfactant, (b) oxidative bleaching agent, and (c) bactericide.

<24> The determination method described in <22> or <23>, wherein (a) the surfactant is selected from linear alkylbenzenesulfonic acid and its salt, fatty acid and its salt, polyoxyethylene alkyl ether, and alkyl glycoside , alkylamine oxide, quaternary ammonium salt, and one or more of alkylamine ethylene oxide adducts.

<25> The method for determining any one of <22> to <24>, wherein (b) the oxidative bleaching agent is hypochlorite.

<26> The determination method described in any one of <22> to <25>, wherein (c) the bactericide is one or more kinds selected from bactericidal alcohols and bactericidal organic acids.

<27> The determination method according to any one of <22> to <26>, wherein (c) the fungicide is one or more selected from ethanol, propanol, lactic acid, and a salt of lactic acid.

＜28＞ The determination method as described in any one of <21> to <27>, wherein the sanitation treatment includes: bringing the above-mentioned sanitation treatment agent into contact with the treated place, and wiping the place in contact with the above-mentioned sanitation treatment agent by a flexible liquid-absorbent material processing premises.

<29> The determination method as described in <28>, which is to bring the above-mentioned sanitation treatment agent into contact with the treatment place by at least one of the following methods: making the flexible liquid-absorbent material impregnated with the above-mentioned sanitation treatment agent contact the treatment place, and Spray the sanitizer mentioned above to the treatment area.

<30> A method of sanitation, which is to determine at least any one of the place where sanitation is performed and the conditions for sanitation by the determination method described in any one of <1> to <29>, and based on the determination Sanitize.

<31> The sanitizing method described in <30>, wherein the sanitizing is treatment using at least one sanitizing agent selected from the group consisting of cleaning, sterilizing, and killing viruses.

<32> The sanitizing method described in <31>, wherein the sanitizing agent contains one or more selected from (a) a surfactant, (b) an oxidative bleaching agent, and (c) a bactericide.

<33> The sanitizing method according to <31> or <32>, wherein the sanitizing agent contains two or more selected from the group consisting of (a) surfactant, (b) oxidative bleaching agent, and (c) bactericide.

<34> The sanitation method as described in <32> or <33>, wherein (a) the surfactant is selected from linear alkylbenzenesulfonic acid and its salt, fatty acid and its salt, polyoxyethylene alkyl ether, alkyl One or more of glycosides, alkylamine oxides, quaternary ammonium salts, and alkylamine ethylene oxide adducts.

<35> The sanitation method according to any one of <32> to <34>, wherein (b) the oxidative bleaching agent is hypochlorite.

<36> The sanitizing method according to any one of <32> to <35>, wherein (c) the bactericide is at least one selected from bactericidal alcohols and bactericidal organic acids.

<37> The sanitizing method according to any one of <32> to <36>, wherein (c) the bactericide is one or more selected from ethanol, propanol, lactic acid, and a salt of lactic acid.

<38> A sanitation treatment set, which includes: a mechanism for detecting amylase in the environment, and a medicament for treating a sanitation treatment place determined according to the detection result of amylase obtained by the institution.

<39> An inference method, which is to detect amylase in the environment, and based on the detection results, it is estimated that there is a high possibility of harmful viruses and/or harmful bacteria.

<40> An inference method, which is to detect amylase in the environment, and based on the detection results, it is estimated that there is a high possibility of harmful viruses and/or harmful bacteria, and the route of infection is estimated.

<41> The estimation method described in <39> or <40>, wherein the harmful virus is an enveloped virus.

<42> A method for displaying the pollution status in a sanitation waiting room, which comprises the following steps: (a) The step of specifying one or more places in the environment, which includes the following steps (i) to (iii): (i) The step of generating location data that can identify the location where the physical object is installed, so as to identify the above-mentioned one or more locations within the defined area; (ii) the steps of recording the location data of the above-mentioned one or more premises in the above-mentioned environment; and (iii) The step of distinguishing and recording the location and location of the physical objects associated with the location data of the above-mentioned one or more premises in the above-mentioned environment; (b) the step of recording the test results of amylase detected from one or more places in the above-mentioned environment; (c) The step of recording the detection results generated according to the distinguished position and part of the physical object recorded in (iii) of (a) above; and (d) A step of displaying the record in (c) together with the location data of the above-mentioned one or more premises in the above-mentioned environment.

<43> A display method of a sanitation place, which determines the sanitation place based on the pollution status in a sanitation candidate place displayed by the display method of <42>, and displays it as the sanitation place.

<44> The method of displaying the sanitary treatment place as described in <43> is to determine the possibility of contamination caused by viruses and/or bacteria based on the above-mentioned pollution status, and determine the sanitary treatment place based on the judgment result.

Example <Example 1 and Comparative Example 1> (1) Embodiment 1 Contamination risk is determined for commercial facilities (shopping malls). Divide commercial facilities into 3 areas (1) store area, (2) catering area, and (3) rest area, and conduct amylase testing in the inspection premises of each area. According to the test results, the average amount of amylase and the positive rate of amylase in different areas are calculated, and the pollution risk is determined based on the following Table 1. The results are shown in Table 2.

In each inspection place, wipe a 25 cm ² area with a clean cotton swab. The detection of amylase from the wiped cotton swab is carried out using an immunochromatographic kit (Independent Forensics Company, RSID (registered trademark) SALIVA [Model: 0100]). When no amylase is detected in the object inspection place, It was set as negative "-", and when amylase was detected in the subject inspection place, it was set as positive "+". When it is positive, visually evaluate in 3 stages according to the depth of the band of the test line, and set it as the amount of amylase. When the band was barely recognized, it was set as +1, when the band was clearly recognized, it was rated as +2, and when a dark band was recognized, it was rated as +3. As the value of + increases, it can be judged that the amount of amylase attached increases. Furthermore, as a result of measuring the amylase solution with a known concentration, regarding the amount of amylase corresponding to each judgment result, "-" is less than 10 ng per 25 ^cm2 area, and "+1" is 10 ng or more and less than 25 ng, "+2" means more than 25 ng and less than 60 ng, "+3" means more than 60 ng.

Furthermore, the above inspection method can be implemented manually, and a set suitable for the content of manualization can be used, for example, a set suitable for implementation under the following conditions (hereinafter referred to as manual example 1). Wetting solution: 0.1 ml of an aqueous solution with a pH value of 7 obtained by adding 0.05% Tween80 to distilled water and sealing it in a 0.5 ml microtube Collection part: A cotton swab with a weight of absorbent cotton wound on one end (excluding the indication part) of 30 mg and a length of 75 mm Extract: 0.1 ml of an aqueous solution with a pH value of 7 obtained by making distilled water contain 0.05% Tween80 and sealing it in a squeezeable tube Collection method: When in use, put a cotton swab into a microtube to absorb the wetting fluid.

In the case of using the steps of the above conditions, select a specific area from the surface of the inspection object, such as the surface of the object object, such as an area of 50 mm × 50 mm, and make the wet collection part vertical and horizontal, and then inclined to the right and left. (the sequence of vertical, horizontal and inclined is not limited) contact. The contact is preferably in the form of equal contact with the surface of the cotton swab, for example, understanding the cotton swab as a square column and distributing each direction to each surface, etc. for contact, but as long as the liquid in the collection part will not be exhausted and will not be used Contact in an uneven manner is sufficient. The carrying part of the collected cotton swab can be directly put into an appropriate container, such as a plastic container or sample bottle with a sealing strip, and the extraction solution can also be added to the container. Extraction of amylase (acquisition of liquid containing amylase) and detection and quantification of amylase are carried out from the carrying part of the collected cotton swab or the like by the following method. After collection, the sample can also be transferred to another place and brought back to the working room for extraction. Extraction method: Soak the collection part of the cotton swab in the extraction solution, and rub the cotton ball from the top of the tube. Detection method: For example, mix 0.08 mL of the attached Running buffer with 0.02 mL of the extract, and add dropwise to a commercially available immunochromatography kit (Independent Forensics, RSID (registered trademark) SALIVA [Model: 0100]) , for the detection of amylase. Quantitative method: Amylase can also be quantified according to the depth of the band at the detection site by reducing the error of the above-mentioned collection method. The serial dilution of the amylase solution with known concentration can be measured, and the depth of the band can be used as a color scale for visual judgment, and a commercially available immunochromatography reader can also be used for quantification. Furthermore, the above collection method can also be set in a manner that facilitates processing by a commercially available amylase assay kit.

(2) Comparative Example 1 The ATP (Adenosine triphosphate, adenosine triphosphate) amount (RLU (Relative Light Units, relative light unit)) was measured in the inspection premises in each area of the same commercial facility as in Example 1. According to the measurement results, the average value of ATP in different regions was calculated. The results are shown in Table 2. In addition, regarding the amount of ATP, the LuciPac Pen manufactured by Kikkoman Biochemifa Co., Ltd. wiped about 25 cm ² of the subject area, and measured it using the company's Lumitester Smart according to the method attached to the product.

[Table 1] ＜Contamination Risk Degree＞ average amount of amylase Not up to +1 More than +1 but less than +2 +2 or more Amylase positive rate Less than 33% e d c More than 33% and less than 66% d c b More than 66% c b a

[Table 2] commercial facilities Example 1 Comparative example 1 inspection area check premises Amount of amylase Average amount of amylase in different regions Amylase positive rate in different regions (%) Pollution Risk in Different Regions ATP amount (RLU) Average ATP amount (RLU) in different regions store area Shopping Basket Handle(1) +1 +0.11 11 e 17973 5923 Shopping Basket Handle(2) - 3936 floor (1) - 18860 floor (2) - 1624 Cash register partition - 1517 Customer Touch Panel (1) - 4104 Customer touch panel(2) - 2172 Escalator handrail - 2366 Toilet cleaning operation button ^*1 - 757 dining area table(1) +1 +1 75 b 70548 21650 table(2) +3 4825 table(3) +1 41600 chair(1) +1 3331 chair(2) +1 6020 Partition(1) - 8990 Partition(2) - 4090 Partition(3) +1 33792 rest area Chair +2 +1 75 b 4877 4945 table - 2189 sofa +1 9449 Side table +1 3266 *1 Cleaning operation button for washing the toilet seat with warm water

The average ATP amount of Comparative Example 1 includes not only viruses and/or bacteria, but also other organic substances such as stains. On the other hand, as shown in Reference Example 3 below, it was suggested that there is a correlation between the amount of amylase and the amount of virus. Therefore, it can be judged that the reliability of the pollution risk degree obtained from the amylase detection result in Example 1 is higher than the reliability of the pollution risk degree obtained from the average ATP amount in Comparative Example 1. In Example 1, by measuring the amount of amylase, it can be estimated that amylase-contaminated places, that is, places with high possibility of contamination caused by components containing amylase. It cannot be deduced from the amount of ATP. For example, in Comparative Example 1 of Table 2, the ATP amount of 18860 RLU of the floor (1) is greater than the ATP amount of 17973 RLU of the shopping basket handle (1), but in Example 1, the amount of amylase of the shopping basket handle (1) Greater than the amount of amylase on the floor (1). According to the results of Example 1, the sanitation treatment of environmental surface contamination caused by amylase and amylase-containing components can be implemented only in places where amylase is detected, which can improve work efficiency. Also, based on the results of Example 1, it is possible to infer places where there is a higher possibility of harmful viruses and/or harmful bacteria, and then infer the route of infection to humans. In Example 1, areas are set in the manner of store area, catering area, and rest area, and the risk of contamination is evaluated based on the detection results of amylase in each area. As a result, compared with the store area, the pollution risk of the dining area and the rest area is greater. Therefore, a more costly sanitation effort (material or manpower) can be proposed for the catering area and the rest area. If the average ATP amount in different areas is calculated as shown in Comparative Example 1, the value of the store area is greater than the value of the rest area, which is different from the tendency of Example 1.

<Example 2 and Comparative Example 2> (1) Example 2 Contamination risk is determined by taking hotel rooms as objects. For 8 guest rooms in the same hotel, in the inspection place shown in Table 3, the amount of amylase was measured in the same manner as in Example 1, and the average value of the 8 guest rooms was obtained as the average amylase amount. Furthermore, the amylase positive rate was obtained from the measurement results, and the degree of contamination risk was determined based on Table 1 above. The results are shown in Table 3. In addition, an example of the state of contamination based on the results of Example 2 is schematically shown in FIG. 2 . Figure 2 schematically shows the overview of hotel rooms and inspection locations, and the black circles marked in the inspection locations indicate the average amount of amylase. The size of the black circle is related to the average amount of amylase, the larger the black circle, the more the average amount of amylase.

(2) Comparative example 2 In the examination place of 8 guest rooms in the same hotel as in Example 2, the ATP amount (RLU) was measured in the same manner as in Comparative Example 1. According to the measurement results, calculate the average value of ATP in different inspection places. The results are shown in Table 3.

[table 3] Hostel room Example 2 Comparative example 2 check premises Average amount of amylase [average of 8 rooms] Amylase positive rate (%) pollution risk ATP volume (RLU) [average of 8 rooms] door handle +0.75 75 c 5919 hanger in closet +0.13 13 e 2321 fridge handle +0.38 25 e 3688 bathroom door handle +0.00 0 e 1109 table(1) +0.63 50 d 924 table(2) +0.50 38 d 1399 Chair +1.00 71 b 2488 lighting switch +1.25 88 b 1251 tv remote +1.38 88 b 2268 Glass for the washbasin +2.00 80 a 2676 sink faucet handle +1.38 88 b 4125 Sink edge of washbasin +2.00 100 a 911

Based on the judgment of the pollution risk in Example 2, the ranking of the places where careful sanitation treatment should be implemented or the places where sanitation treatment should be given priority can be proposed. On the other hand, in Comparative Example 2, for example, the amount of ATP at the sink edge of the washbasin with the highest pollution risk in Example 2 is the least, which is different from the tendency of Example 2. Also, based on the results of Example 2, it can be estimated that there is a higher possibility of harmful viruses and/or harmful bacteria, and then the route of infection to humans can be estimated. The pollution status in Fig. 2 can be displayed by the output device of the information processing device (PC, smart phone, etc.). By displaying the pollution status as shown in FIG. 2 , it is easy to visually grasp the relationship between the inspected place and the pollution status. Furthermore, a sanitation place can be determined based on the pollution situation shown in FIG. 2, and can be displayed as a sanitation place.

＜Reference example 1＞ The table (inspection area: a table different from the inspection place (1), (2), (3) in the catering area) of the commercial facility that has been tested for amylase in Example 1 is used as the test object to implement the effect of sanitation treatment. evaluate. Use a rag moistened with tap water, or a rag moistened with a commercially available medical detergent (Clean Keeper for Medical Use, Kao Co., Ltd.) diluted 200 times with tap water, in the vertical and horizontal directions Sanitize the table by wiping it down once respectively. Wiping with a rag moistened with tap water and a rag moistened with a commercially available medical lotion was carried out on different tables. Before and after sanitation, the amount of amylase was measured in the same manner as in Example 1, and the amount of ATP was measured in the same manner as in Comparative Example 1. The results are shown in Table 4.

[Table 4] Reference Example 1A sanitation method Wiping with medical detergent Wipe with water Amount of amylase before processing +1 +1 after treatment - +1 Reference Example 1B sanitation method Wiping with medical detergent Wipe with water ATP amount (RLU) before processing 37391 16327 after treatment 11215 10704

According to the results in Table 4, it can be judged that in the equipment of this facility, the effect of reducing the amount of amylase is higher by wiping and cleaning with medical detergent than by wiping with water.

＜Reference example 2＞ For (1) the situation of sneezing indoors, (2) the situation of three people eating on the table indoors, and (3) the situation of using personal computers for web conferencing indoors, the detection of amylase was carried out. Regarding (1), in a room with closed doors and windows, a subject stood up and sneezed 5 times without wearing a mask, directly below the subject, at a distance of 50 cm from the subject , 1 m, and 2 m for the detection of amylase. Regarding (2), 3 subjects sat at a table for four (without spacers), talked and ate for 30 minutes without wearing a mask, and tested amylase between 2 people side by side in the middle of the table . With regard to (3), one subject sat in front of a table and used a personal computer for 2 hours and 30 minutes of web conferencing while wearing a mask, and tested amylase on the keyboard of the personal computer. The detection of amylase was carried out in the same manner as in Example 1. Where more amylase was detected was where there was a higher probability of staining from saliva. The results are shown in Table 5.

[table 5] Behavior (1) Sneezing (2) Eat while talking (3) web conferencing details Not wearing a mask, 5 times Without a mask, 30 minutes Wearing a mask, 2 hours and 30 minutes inspection site directly below the subject 50 cm place 1 m place 2 m place between the tables between 2 people PC keyboard Amount of amylase +3 +3 +1 - +1 +2 +1

How the saliva droplets will scatter under the conditions of (1) to (3) above can be predicted by, for example, simulation using a supercomputer "Fuyue". It can be confirmed that the results in Table 5 correlate with such predicted results. Therefore, it is considered that the place where amylase is detected in Examples 1 and 2 is highly likely to be contaminated by saliva droplets spit out from the mouth.

<Reference Example 3> A phage MS2 solution (10 ¹² PFU/mL) was mixed in saliva so that the concentration of phage MS2 became 10 ¹⁰ PFU/mL. Measure 0.1 mL of this solution, spread it on a PP (polypropylene, polypropylene) plate to a range of 4 cm×4 cm, and let it dry in a biological safety cabinet for 1 hour. Make it the initial attachment location. Proceed as follows from the initial attachment location. Gently wipe any part of the initial attachment place with a clean finger, and gently wipe a new uncontaminated PP board with the finger part. This operation was repeated 5 times. The uncontaminated PP board is wiped within the range of 4 cm × 4 cm. Set the wiped place in the new PP board as the propagation place (1). Carry out the same operation on another new PP board from the propagating place (1) and set it as the propagating place (2). Furthermore, carry out the same operation on another new PP board from the propagating place (2), and set it as the propagating place (3). Use a sterilized cotton swab moistened with sterilized water to wipe each place of the initial attachment place and the propagation place (1)-(3). Soak a cotton swab in 0.2 mL of the extraction buffer (RSID (registered trademark) SALIVA Extraction Buffer) attached to the kit to prepare an extraction solution of the swabbed component. 0.1 mL of the extract was used for the determination of the amount of virus by the following plaque assay, and the remaining part was used for the determination of the amount of virus by the immunochromatography kit (Independent Forensics Company, RSID (registered trademark) SALIVA [Model: 0100]). Determination of the amount of amylase performed. The results are shown in Table 5.

[Method of Plaque Assay] Autoclaved LB (Lysogeny broth) agar medium (agar concentration: 1.5%) to which calcium chloride was added so that the final concentration became 2.25 mM, and poured 15 mL into The petri dish (Nissui Pharmaceutical Co., Ltd., Nissui sterilized shallow petri dish [model number: 06300]) was allowed to stand at room temperature to solidify. After autoclaving LB agar medium (0.6% agar concentration) to which calcium chloride was added so that the final concentration was 2.25 mM, keep it at about 40°C, and take 4 mL and 0.9 mL of Escherichia coli liquid (OD600=0.4) , 0.1 mL of the above extracts were mixed, poured into the prepared petri dish solidified with LB agar medium for layering. This was cultured statically at 37°C overnight, and the number of virus-infected and lysed parts (plaques) among Escherichia coli on the agar was counted as the amount of virus (PFU/cm ² ).

[Table 6] Virus amount (PFU/cm ² ) Amount of amylase test methods plaque assay Immunochromatography initial attachment space more than 100,000 +3 Spread Premises (1) 680 +3 Spread Premises (2) 2 +1 Spread Premises (3) 0 -

By the method of this reference example, the spread of saliva caused by hand contact can be confirmed. Therefore, it is suggested that the place where amylase is detected in Examples 1 and 2 is not only the place where droplets adhere, but also the place where saliva spread through contact with objects such as hands. Also, since it was confirmed in this reference example that the amount of virus and the amount of amylase have a tendency to change due to transmission, it is considered that the amylase is detected in the actual environment regardless of the attachment method of the amylase (from droplets) Transmission by attachment or contact) are places where there is a risk of contamination by amylase-containing components, such as saliva, or even viruses and/or bacteria in saliva. In addition, the results suggest that the detection results of amylase can be used to deduce the infection route of viruses and/or bacteria.

＜Reference example 4＞ Multiple sheets were prepared by applying 5 μL of 20-fold diluted saliva (collected from a 30-year-old male) onto a polypropylene substrate and drying it. Amylase assays were performed from each substrate according to Example 1 of the above manual. Among them, the cotton swabs used as the collecting part were (1) those whose support body was made of wood (manufactured by Nippon Cotton Swab Co., Ltd., cotton swab CTB-15 with test tube), and (2) those whose support body was made of resin (manufactured by Eiken Chemical Co., Ltd., γ Collect swab (collect swab R, TC2400). In addition, the amylase quantification system is divided into the following three groups: (I) the group for quantification immediately after collection; (II) the group for quantification after the collected cotton swabs are stored at 4°C for 4 days or 10 days; (III) ) The group in which the collected cotton swabs were stored at 25°C for 4 days or 10 days and then quantified. Preservation is carried out in the test tube attached to each cotton swab. The amount of amylase was quantified by the same method as in Example 1. The results are shown in Table 7.

[Table 7] Amount of amylase Quantification immediately after acquisition Store the swab at 25°C for 4 days before quantification The swabs were stored at 25°C for 10 days before quantification Store the swab at 4°C for 4 days before quantification The swabs were stored at 4°C for 10 days before quantification Support for cotton swabs wooden +2 - - +2 +1 Resin +2 +2 +1 +2 +2

As can be seen from Table 7, when a cotton swab made of wood is used as the support, the detection sensitivity decreases when stored at 25°C. It can be seen that when the support is made of wood, when stored at 4°C, the detection strength can be maintained for 4 days, but the detection sensitivity decreases for 10 days. Therefore, when the support is made of wood In some cases, it is preferable to store at a low temperature. On the other hand, it can be seen that when the support is a cotton swab made of resin, the detection sensitivity can be maintained for 4 days even at 25° C. , the support of the cotton swab is preferably made of resin rather than wood.

＜Reference example 5＞ By the same method as in Reference Example 4, a plurality of substrates on which saliva was adhered was produced, and by the same method as in Reference Example 4, amylase was quantified from each substrate. Among them, the cotton swab is made of wood as a support body (Cotton Swab CTB-15 with Test Tube, manufactured by Nippon Cotton Swab Co., Ltd.). In addition, regarding the quantification of amylase, after collecting with a cotton swab, the amylase was extracted immediately, and the amylase was quantified in the following two groups: (i) The group that quantified after storing the extract at 4°C for 4 days or 10 days ; (ii) The group in which the extract was stored at 25°C for 4 days or 10 days and then quantified. The results are shown in Table 8.

[Table 8] Amount of amylase Quantification of extracts immediately after collection The extract was stored at 25°C for 4 days before quantification The extract was stored at 25°C for 10 days before quantification The extract was stored at 4°C for 4 days before quantification The extract was stored at 4°C for 10 days before quantification +2 +2 +1 +2 +2

As can be seen from Table 8, when the extract collected from a cotton swab is stored for 4 days before amylase quantification, the detection sensitivity can be maintained regardless of whether it is at 4°C or 25°C. In addition, it can be seen that since the detection sensitivity can be maintained for a long time by storage at low temperature, it is preferable to store at low temperature when the step of storing the extract is included.

＜Adjustment example＞ In Tables 9-11, the preparation example of the sanitization agent which can be used for the sanitization of this invention is shown. These sanitizing agents can be used to carry out the sanitizing method of the present invention. However, the sanitizing agent is not limited to these. Table 9 lists the treatment agents that are mainly impregnated in sheet-like materials such as non-woven fabrics, and can be used to obtain sheet-like sanitation treatment agent products. Sheet-shaped sanitizing agent products are suitable for contacting and wiping the sanitizing agent and the treated place. Table 10 shows treatment agents that are mainly used for spraying with a nebulizer or the like, and can be used to obtain a spray-type sanitation treatment product. Spray-on sanitizer preparations are suitable for efficiently bringing the sanitizer into contact (spraying) with the treatment area. After contacting the sanitation agent with the spray sanitation agent product, the treated place can be wiped with flexible materials such as non-woven cloth and sponge as needed. Table 11 lists the treatment agents mainly used for the purpose of cleaning and sterilizing, and the products can adopt various forms. For example, it can be used in sheet-form sanitation preparations or spray-on sanitation preparations as described above.

[Table 9] Sanitation agent for sheet products Deployment example A1 Deployment example A2 Deployment example A3 Deployment example A4 Deployment example A5 Blending ingredients (mass%) (a) Amphitol 20N 0.2 0.2 0.1 0.5 Secondary alcohol EO 3 moles 0.2 Alkyl glycoside 0.01 Rheodol TW-L120 0.2 Sanisol B-50 0.2 0.2 0.5 0.2 (b) sodium hypochlorite 0.2 (c) ethanol 2 5 (d) lactic acid 0.5 other ingredients MFG 0.1 2 10 15 Carbopol ETD 2020 PLYMER 0.1 Defoamer (silicone) 0.01 0.01 Zinc sulfate 10 preservative 0.01 sodium benzoate 0.2 0.2 water The remaining part The remaining part The remaining part The remaining part The remaining part total 100 100 100 100 100 pH value(20℃) 5 9 7 12 7

[Table 10] Hygiene treatment agent for spray products Deployment example B1 Deployment example B2 Deployment example B3 Deployment example B4 Deployment example B5 Blending ingredients (mass%) (a) Amphitol 20N 0.2 0.5 0.2 3 Amphitol 20AB 1 Fatty acid (carbon number 8-14) 0.6 Secondary alcohol EO 3 moles 1.5 0.05 Alkyl glycoside 0.1 0.1 Penetol GE-EH 0.1 0.1 Sanisol B-50 0.1 0.01 0.005 1 0.5 (c) ethanol 0.2 3 12 5 (d) lactic acid 0.4 other ingredients BDG 8 MFG 3 citric acid 0.001 0.01 1 2 EDTA (Ethylene Diamine Tetraacetic Acid, ethylenediamine tetraacetate) 3 0.2 betaine polymer 0.1 Carbopol ETD 2020 PLYMER 0.02 Defoamer (silicone) 0.0001 0.005 Zinc sulfate 0.1 sodium benzoate 0.1 water The remaining part The remaining part The remaining part The remaining part The remaining part total 100 100 100 100 100 pH value(20℃) 5 6 7.5 8 8

[Table 11] Detergent Deployment example C1 Deployment example C2 Deployment example C3 Deployment example C4 Deployment example C5 Blending ingredients (mass%) (a) Amphitol 20N 5 20 Neopelex GS 0.5 Emal 20CS 0.5 Fatty acids (C8-C14) 0.5 0.5 Alkyl glycoside 5 Rheodol TW-L120 0.2 Laurylamine EO adduct (2 moles) 5 Sanisol B-50 5 (b) sodium hypochlorite 3 (c) ethanol 60 (d) lactic acid 0.1 other ingredients BDG 1 citric acid 0.5 malic acid 0.5 Preservative (Kason CG) 0.001 0.01 sodium benzoate 0.1 water The remaining part The remaining part The remaining part The remaining part The remaining part total 100 100 100 100 100 pH value(20℃) 7 7 3.5 7 12

Components (parts) used in Tables 9 to 11 are as follows. ・Amphitol 20N: manufactured by Kao Co., Ltd., lauryl dimethyl amine oxide ・Amphitol 20AB: manufactured by Kao Co., Ltd., lauryl amidopropyl betaine ・Neopelex GS: Manufactured by Kao Co., Ltd., alkylbenzenesulfonic acid ・Emal 20CS: Made by Kao Co., Ltd., sodium polyoxyethylene lauryl ether sulfate ・Secondary alcohol EO 3 moles: Nonionic surfactant obtained by adding an average of 3 moles of ethylene oxide to secondary alcohols (carbon number 12-14), Softanol 30, manufactured by Nippon Shokubai Co., Ltd. ・Alkyl glucoside: AG-124, manufactured by Kao Co., Ltd., nonionic surfactant ・Rheodol TW-L120: Made by Kao Co., Ltd., polyoxyethylene sorbitan monolaurate ・Laurylamine EO adduct (2 moles): a nonionic surfactant obtained by adding an average of 2 moles of ethylene oxide to laurylamine ・Penetol GE-EH: Manufactured by Kao Co., Ltd., 2-ethylhexylglyceryl ether ・Sanisol B-50: Manufactured by Kao Co., Ltd., alkyl benzyl dimethyl ammonium chloride ・BDG: Diethylene glycol butyl ether (diethylene glycol monobutyl ether), product made in Nippon Emulsifier Co., Ltd., solvent ・MFG: Propylene glycol monomethyl ether, manufactured by Nippon Emulsifier Co., Ltd., solvent ・Betaine polymer: Copolymer produced by the method of Example and Production Example 1 of Japanese Patent Laid-Open No. 2018-199770 ・Carbopol ETD 2020 PLYMER: Carbopol ETD 2020 PLYMER: Carboxyvinyl polymer manufactured by Lubrizol Advanced Materials ・A preservative: Casson CG, product made in DuPont

Fig. 1 is a flow chart of the processing steps of the method for displaying the pollution status of the present invention. Fig. 2 is a schematic diagram showing an example of the method of displaying the pollution status of the present invention.

Claims (22)

A method for determining a place for sanitation, which is to detect amylase in the environment, and determine a place for sanitation based on the detection result. The method for determining the sanitation treatment place as in Claim 1 is to determine the possibility of contamination caused by viruses and/or bacteria based on the amylase test results, and determine the sanitation treatment place based on the judgment results. A method for determining sanitation treatment conditions is to detect amylase in the environment and determine sanitation treatment conditions based on the detection results. As in the determination method of claim 3, it is based on the detection result of amylase to determine the possibility of contamination caused by virus and/or bacteria, and determines the conditions of sanitation treatment according to the determination result. A method for determining sanitation treatment method, which is to detect amylase in the environment, and determine the place where sanitation treatment is performed and the conditions of sanitation treatment based on the detection results. As in the determination method of Claim 5, it is based on the detection result of amylase to determine the possibility of contamination caused by viruses and/or bacteria, and based on the determination results to determine the sanitation treatment place and/or the sanitation treatment conditions . The determination method according to any one of Claims 1 to 6, wherein the sanitation treatment includes one or more treatments selected from the group consisting of purification, antibacterial adhesion prevention treatment, and virus adhesion prevention treatment. The determination method according to any one of claims 1 to 7, which is to detect amylase by utilizing antigen-antibody reaction and/or enzyme reaction. The method for determining any one of Claims 1 to 8, wherein the environment is a living environment. The determination method according to any one of claims 1 to 9, wherein the environment includes an object to be detected for amylase (hereinafter referred to as an object to be detected), and the amylase on the surface of the object to be detected is detected. The determination method according to any one of Claims 1 to 10, wherein the sanitizing treatment is a treatment using at least one sanitizing agent selected from cleaning, sterilizing and killing viruses. The determination method of claim 11, wherein the sanitizing agent contains one or more selected from (a) surfactant, (b) oxidative bleaching agent, and (c) bactericide. The determination method of claim 11 or 12, wherein the sanitation treatment includes: contacting the above-mentioned sanitation treatment agent with the treatment place, and wiping the treatment place in contact with the above-mentioned sanitation treatment agent by a flexible liquid-absorbent material. A method of sanitation, which is to determine at least any one of the place where the sanitation is to be performed and the conditions for the sanitation by the determination method according to any one of claims 1 to 13, and perform sanitation based on the determination. The sanitizing method according to claim 14, wherein the sanitizing treatment is a treatment using at least one sanitizing agent selected from cleaning, sterilizing and killing viruses. The sanitizing method according to claim 15, wherein the sanitizing agent contains one or more selected from (a) surfactant, (b) oxidative bleaching agent and (c) bactericide. A sanitation treatment set, which includes: a mechanism for detecting amylase in the environment, and a medicament for treating a sanitation treatment place determined according to the detection result of amylase obtained by the institution. An inference method, which is to detect amylase in the environment, and based on the detection results, it is estimated that there is a high possibility of harmful viruses and/or harmful bacteria. An inference method, which is to detect amylase in the environment, and based on the detection results, it is estimated that there is a high possibility of harmful viruses and/or harmful bacteria, and the route of infection is estimated. A method for displaying the pollution status in a sanitation waiting room, which comprises the following steps: (a) The step of specifying one or more places in the environment, which includes the following steps (i) to (iii): (i) The step of generating location data that can identify the location where the physical object is installed, so as to identify the above-mentioned one or more locations within the defined area; (ii) the steps of recording the location data of the above-mentioned one or more premises in the above-mentioned environment; and (iii) The step of distinguishing and recording the location and location of the physical objects associated with the location data of the above-mentioned one or more premises in the above-mentioned environment; (b) the step of recording the test results of amylase detected from one or more places in the above-mentioned environment; (c) The step of recording the detection results generated according to the distinguished position and part of the physical object recorded in (iii) of (a) above; and (d) A step of displaying the record in (c) together with the location data of the above-mentioned one or more premises in the above-mentioned environment. A display method of a sanitation treatment place, which determines the sanitation treatment place based on the pollution status in the sanitation treatment candidate place displayed by the display method according to claim 20, and displays it as the sanitation treatment place. The display method of sanitation treatment place as in claim 21 is to judge the possibility of contamination caused by virus and/or bacteria based on the above-mentioned pollution status, and determine the sanitation treatment place according to the judgment result.

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