JP2023049019A - Method for determining sanitary treatment point - Google Patents

Abstract

To provide a method for determining sanitary treatment that can easily and reliably determine where and under what conditions sanitary treatment such as washing, disinfection, virucidal, and sterilization can be effectively performed.SOLUTION: A method for determining a sanitary treatment point includes detecting amylase in an environment, and determining a point to be sanitized based on a detection result.SELECTED DRAWING: None

Description

The present invention provides a sanitary treatment location determination method, a sanitary treatment condition determination method, a sanitary treatment method determination method, a sanitary treatment method, a sanitary treatment kit, an estimation method, a method for displaying the state of contamination at candidate sanitary treatment locations, and a sanitary treatment. It is related with the display method of a part.

The living environment surrounding us includes various facilities, facilities, devices, etc., and various treatments are performed in consideration of their respective uses. For example, in commercial facilities, accommodation facilities, etc., processes such as cleaning and sterilization are performed in order to maintain sanitary conditions. At that time, it has been proposed to determine the degree of contamination and to determine processing conditions according to the degree of contamination.

Patent Document 1 discloses a cleaning method comprising a predetermined detection method for detecting a test substance such as a virus, and a cleaning step for removing the test substance from a target space according to the detection result of the detection method. It is disclosed (claim 19).

Patent Document 2 discloses a method for evaluating the sanitary conditions of a living environment, which includes a step of detecting the presence or absence of Methylobacterium bacteria in the living environment.

Patent Document 3 describes an installation step of arranging a specific toilet dirt detection sheet or a specific toilet dirt detection sheet laminate at a plurality of locations in a toilet area to be cleaned, and detecting stains at the plurality of locations from the discoloration state of the installed sheets. and a timing determination step of determining the timing for cleaning the toilet according to the determined degree of contamination, and a toilet cleaning range determination method are disclosed. there is

JP 2015-206671 A JP 2012-5455 A JP 2013-36189 A

In the environment, when performing treatments such as cleaning, bleaching, deodorizing, viricidal, and sterilizing, chemicals are often used according to the treatment. In large facilities, it is inefficient to apply drugs to all sites. In other words, in such a large-scale facility, labor and costs can be greatly reduced if it is possible to efficiently determine the locations and times for cleaning and other treatments. In particular, it is important to prevent infection by viruses and bacteria in facilities used by an unspecified number of people, so an effective method for reliably and efficiently performing treatment is desired.

The present invention provides a method for determining sanitary treatment that can easily and reliably determine locations and conditions where sanitary treatment such as cleaning, disinfection, virucidal, and sterilization can be effectively performed.

TECHNICAL FIELD The present invention relates to a method for determining sanitary treatment sites, which detects amylase in the environment and determines sanitary treatment sites based on the detection results.

The present invention also relates to a sanitary treatment condition determination method for detecting amylase in the environment and determining sanitary treatment conditions based on the detection results.

The present invention also relates to a method for determining a sanitary treatment method, comprising detecting amylase in the environment, and determining a location for sanitary treatment and sanitary treatment conditions based on the detection results.

The present invention also relates to a sanitary treatment method, in which at least one of a location to be sanitized and conditions for sanitary treatment are determined by the determination method of the present invention, and sanitary treatment is performed based on the determination.

The present invention also relates to a sanitary treatment kit comprising a means for detecting amylase in the environment and a chemical used for sanitizing sites determined by the amylase detection results obtained by the means.

The present invention also relates to an estimation method for detecting amylase in the environment and estimating locations where harmful viruses and/or harmful bacteria are likely to exist based on the detection results.

The present invention also relates to an estimation method for detecting amylase in the environment, estimating locations where harmful viruses and/or harmful bacteria are likely to exist based on the detection results, and estimating infection routes.

Further, the present invention is a method for displaying a contamination state at a candidate site for sanitary treatment, comprising:
(a) identifying one or more locations in the environment, comprising:
a step including the step of
(i) generating location data capable of identifying the location of the entity to identify the one or more locations within the defined area;
(ii) recording location data for said one or more locations within said environment; a step of separately recording;
(b) recording amylase detection results from one or more locations within the environment;
(c) recording the generated detection result for each segmented position and part of the physical entity recorded in (iii) of (a); and (d) recording the one or more records of (c) in the environment. a step of displaying together with the position data of
A method for displaying a contamination situation at a candidate site for sanitary treatment, comprising:

The present invention also relates to a method of displaying sanitary treatment locations, wherein the sanitary treatment location is determined based on the contamination status of the sanitary treatment candidate location displayed by the display method of the present invention and displayed as the sanitary treatment location.

According to the present invention, there is provided a method for determining a sanitary treatment location, sanitary treatment conditions, or a sanitary treatment method that can easily and reliably determine a location where sanitary treatment such as cleaning, disinfection, virucidal, and sterilization can be effectively performed. be done. Further, the present invention provides a method for easily and reliably estimating the location and infection route of harmful viruses and/or harmful bacteria.

1 is a flow chart of a processing procedure for carrying out a method of displaying a contamination state according to the present invention; Schematic diagram showing an example of the display method of the contamination status of the present invention

As used herein, "sanitary treatment" may be a treatment applied to the environment to maintain health, for example, removing "contaminants" from the "environment" and further from "objects", Denotes inactivation of "contaminants" or both.
As used herein, the term "contaminant" may be a substance that poses a health hazard when present in an object, such as saliva, droplets from the oral cavity, and the like. Droplets from the oral cavity may contain saliva and substances associated with saliva, such as amylase. Furthermore, the "substance associated with saliva" may include viruses and/or bacteria (sometimes simply referred to as "bacteria"), as well as harmful viruses and/or harmful bacteria.
As used herein, the term "contamination" refers to, for example, the absence of contaminants such as saliva and saliva-associated substances such as amylase in the "environment" and further in the "object". It may be to be in a situation to exist in profitable quantity.

Amylase is an enzyme contained in human saliva. The present invention finds that the degree of detection of amylase adhered to the surface of the living environment correlates with the possibility of contamination by components containing amylase, and a location with a high degree of detection of amylase is a location with a high possibility of contamination. It was found that contamination by viruses and/or bacteria can be efficiently prevented and removed by determining that location as a location for sanitary treatment such as cleaning, disinfection, viricide, and sterilization. Furthermore, the present invention has found that the possibility of the presence of harmful viruses and/or harmful bacteria and the route of infection can be estimated with high accuracy based on the degree of detection of amylase. Based on the degree of detection of amylase, it has been conventional to determine the possibility of contamination, determine the site to be sanitized, the possibility of the presence of harmful viruses and / or harmful bacteria, and estimate the route of infection. not

The present invention involves the detection of amylases in the environment. The environment may be a living environment, a natural environment, or the like. The environment, especially the living environment, may include buildings, public transportation. Also, buildings and public transportation may each include fixtures associated with them. There are no restrictions on the size or use of the building.

Examples of buildings include buildings selected from commercial facilities, lodging facilities, educational facilities, medical facilities, nursing facilities, business facilities, and eating and drinking facilities, and may be complex facilities of these. Commercial facilities include, for example, shopping centers, supermarkets, cinema complexes, and amusement parks. Accommodation facilities include, for example, hotels, inns, private lodging facilities, and the like. Educational facilities include, for example, various schools and cultural centers. Medical facilities include, for example, hospitals. Nursing facilities include, for example, care houses for the elderly. Business facilities include, for example, office buildings and factories. Eating and drinking establishments include, for example, restaurants, coffee shops, fast food restaurants, and the like.

Public transportation includes public transportation selected from rail vehicles, road vehicles, ships, and aircraft. Railway vehicles include electric trains, diesel vehicles, and the like. Road vehicles include buses and the like. A passenger ship etc. are mentioned as a ship. Airplanes include passenger planes and the like.

In the present invention, the environment includes an object for amylase detection (hereinafter referred to as detection object), and amylase can be detected on the surface of the detection object. Detected objects may include, for example, detected objects selected from buildings, building fixtures, public transport, and public transport fixtures. In the present invention, amylase can be detected on the surface of an article, for example, an article placed in a room, as the object to be detected. The article may be either a fixed article or a non-fixed article. The material of the article is also not limited. Examples of articles include furniture, electrical appliances, plumbing articles, building materials, fittings, clothing, bedding, etc. More specifically, for example, tables, chairs, sinks, toilet seats, doorknobs, taps, switches, Items selected from remote control devices (remote controllers for various devices, etc.), computer input devices (keyboards, mice, etc.), wallpaper, cloth ceilings, curtains, and carpets. In addition, as goods, for example, elevator switches installed in buildings, escalator handrails installed in buildings, ticket vending machines for transportation, straps for vehicles, goods such as shopping baskets and carts frequently used in commercial facilities can be mentioned. In the present invention, amylase can be detected on the surface of these articles, which are objects to be detected.

In the present invention, the environment may be an area to be sanitized (hereinafter also referred to as a target area). In the following, the term target area may mean environment. The target area may be selected from, for example, buildings such as commercial facilities, lodging facilities, and business facilities, transportation public facilities, and the like. A site where amylase is detected is selected from those regions of interest. The site where amylase is detected can be appropriately selected in consideration of the use, scale, etc. of the target region. In the present invention, an area of environmental sanitary treatment can be defined and amylase can be detected in the area of interest. That is, in the present invention, a region to be subjected to sanitary treatment in the environment is determined, and amylase can be detected in the detection target in the target region.

In the present invention, it is preferable to detect the amylase on the surface of the object to be detected in the target region, and to measure the amount of amylase as necessary. The amylase may be human-derived amylase.

A sample for amylase detection from the surface of the detection target in the target region can be collected by bringing an appropriate collection means, such as absorbent cotton, nonwoven fabric, sponge, etc., into contact with the surface of the detection target. In the present invention, for detection of amylase from the surface of the object to be detected, it is preferable to use a collection means having a support and a sample collection section provided on the support. In the present invention, swabs or cotton swabs are preferably used as the sampling means. These may be of medical or specimen collection use. The collecting part can have various shapes such as a spherical shape and a longitudinal shape. Also, the collecting part may be made of, for example, fibers, porous materials, or the like. Specific examples of fibers include natural fibers such as cotton, synthetic fibers, and blended fibers thereof. Sponge etc. are mentioned as a porous material. It is preferable that the collection part has a liquid absorption property. It is preferable to keep the collection part wet with a suitable liquid. In other words, it is preferable that the collecting portion carries the liquid. It is preferable that the amount of the liquid held in the collecting portion is an amount that does not cause dripping. The dry mass of the collection part, for example fibrous material or porous material, is preferably, for example, 10 mg or more and 1000 mg or less. Said liquid to be carried in the collection part may be, for example, water. In addition, the liquid may be an aqueous solution containing a surfactant, a physiological saline solution, a buffer solution, or the like, or may be a composition containing a combination of ingredients blended therein. Detergents may be selected that do not affect amylase detection. Examples of surfactants include nonionic surfactants. Nonionic surfactants include, for example, alkyl groups having 6 to 22 carbon atoms, and the average number of added moles of oxypropylene groups is 0. Polyoxyalkylene alkyl ethers and polyoxyethylene alkylphenyl ethers, and polyhydric alcohols and fatty acids such as glycerin, pentaerythritol and sorbitol, which have an average of 5 or more and an average number of added moles of oxyethylene groups of 5 or more and 50 or less Polyoxyalkylene adducts of esters with and the like can be mentioned. As nonionic surfactants, commercially available products such as Triton X-100, Nonidet P-40 (NP-40), Tween 20 and Tween 80 can be used. The concentration of the surfactant in the liquid may be determined within a range that does not affect amylase detection. The concentration of the surfactant in the liquid is, for example, 2 mass% or less, further 1 mass% or less, further 0.5 mass% or less, further 0.1 mass% or less, further 0.05 mass% or less, further 0 0.01% by weight or less, or even 0.005% by weight or less.

In order to obtain common information by aligning the sampling conditions for each sampling location, for example, a specific part, a room, a building, or an area, it is necessary to carry approximately the same amount of liquid in the same type of sampling part. It is preferred to use means. In other words, since a plurality of sampling means are normally used for sampling samples, each has the same type of sampling part, and the same sampling part is used at the initial stage before contact with the surface of the detection target. It preferably carries an amount of liquid. For this purpose, a plurality of supports, each of which is wetted with a predetermined amount of liquid, may be sealed in an aluminum package or a highly airtight container. In addition, when the sampling part and the initial wetting liquid are separated, put a certain amount of liquid in a microtube or test tube in advance, seal it, open it before use, and bring it into contact with the sampling part to absorb the liquid. A method is also conceivable to prevent water absorption in excess of the amount. In the case of a cotton swab, the amount of liquid carried by the sampling part is preferably 10 mg or more, more preferably 30 mg or more, still more preferably 90 mg or more, and preferably 1500 mg or less, 1000 mg or less, or 500 mg or less. Although the amount of liquid to be absorbed may vary depending on the inspection area and the properties of the collection site, 200 mg or less, more preferably 100 mg, is generally easy to use. The ratio of the liquid to the collection part (for example, the cotton of a cotton swab) is preferably 0.5 or more, more preferably 1 or more, still more preferably 2 or more, even more preferably 3 or more, and preferably 5 or less in mass ratio. , more preferably 4 or less.

Further, the support is specifically made of wood or made of resin. If the amylase is not extracted immediately from the collected cotton swab, for example, if the amylase is taken back to a different laboratory from where it was collected for extraction, amylase extraction becomes difficult, so a support made of resin is preferred. This is thought to be due to the gradual transfer of the liquid from the collection part to the support part in the case of wood. In addition, when amylase is not immediately extracted from the collected cotton swab, the collecting means such as a cotton swab is preferably used at a temperature of 10°C or lower, more preferably 5°C or lower, and preferably 0°C or higher, more preferably 2°C or higher. Saving is preferred. It is preferable to transport, store, etc. the collecting means within this temperature range. At this time, it is preferable to seal and store the cotton swab in a zipped plastic container, sample bottle, aluminum package, or highly airtight container. Also, even if amylase is not detected immediately from the extract collected from the collection part, it is stored at a low temperature, preferably 10° C. or lower, more preferably 5° C. or lower, and preferably 0° C. or higher, more preferably 2° C. or higher. is preferred. It is preferable to transport and store the extract within this temperature range.

In the case of using the above sampling means, the inspection area targeted by one sampling part is preferably 100 mm ² or more, more preferably 2000 mm ² or more when the amount of liquid supported by the sampling part is 90 mg or more and 500 mg or less. is preferably 30,000 mm ² or less, more preferably 10,000 mm ² or less, from the viewpoint of maintaining the wettability of the collection part and maintaining the efficiency of amylase wiping. When the test surface is large, the method of collecting samples by the above-described collecting means assumes a square equivalent to the above area as the target surface, and in each of the vertical, horizontal, and diagonal (two diagonals) directions on that surface It is preferable to collect samples by determining the number of times of contact between 10 and 20 times, that is, the number of times of contact in one direction×4 in total on one surface. If the surface to be inspected is curved or rectangular, a range close to the determined area should be set and samples should be taken.

When the collecting means is used, extraction of amylase from the collecting portion after contact with the surface of the object to be detected is performed by contacting the collecting portion with an extract liquid, for example, extracting liquid contained in an appropriate container. It can be done by immersing in The amount of the extract varies depending on the size of the sampling part, etc. For example, the amount of the extract in the case of a cotton swab is preferably 30 mg or more, more preferably 50 mg or more, and preferably 10000 mg or less, more preferably 10000 mg or less. 5000 mg or less, more preferably 1000 mg or less. Preferably, the amount of the extract is 0.1 times or more, further 0.5 times or more, and 10 times or less, and further 5 times or less, the amount of the liquid impregnated in the collection part on a mass basis. Furthermore, it is less than two times. In the case of immersion, the immersion time is, for example, 10 seconds or more, 1 minute or more, 5 minutes or more, 10 minutes or more, 30 minutes or more, 1 hour or more, 2 hours or more, and less than 48 hours, further 24 hours, 12 hours. hours, may be less than 6 hours. In the case of immersion, the immersion temperature can be a temperature at which amylase is not denatured, for example, room temperature (eg, 10° C. or higher and 30° C. or lower). The extract liquid may have the same composition as the liquid. While the sampling part is immersed in the extract, it may be left still, or an external force may be applied, such as application of ultrasonic waves, stirring with a stirring device such as a vortex mixer, manual shaking, pressing with a rod, or the like. , pressing by contacting the wall surface of the container, or the like may be performed. Moreover, when using a small amount of extract, the liquid may be separated from the collection part by centrifugation, pressing, or the like. Amylase may be detected from the extract obtained by contacting the collection part by such a method, for example, by a method using an antigen-antibody reaction and/or an enzymatic reaction, which will be described later. For amylase collection, extraction, etc., a commercially available kit suitable for this can be used. In that case, amylase may be collected according to the method described in the kit. Moreover, the liquid and the extract may be those attached to the kit.

As one aspect of the present invention, amylase is detected on the surface of an object to be detected in the environment and further in the living environment, and the location to be sanitized is determined based on the detection results. determination method.
As another aspect of the present invention, amylase is detected on the surface of an object to be detected in the environment and the object to be detected in the living environment, and the location to be sanitized is determined based on the detection results. A determination method, wherein a sample is collected from the surface of the object to be detected using a collection means, preferably a swab and/or a cotton swab, which has a support and a sample collection portion provided on the support, and Methods for determining sanitary treatment sites include detection of amylase from a sample.

In the present invention, amylase is detected at different locations in the target region, and a location where relatively more amylase is detected can be selected as a location for sanitary treatment.

In the present invention, amylase is detected in a target region, and the conditions for sanitary treatment, such as the type of treatment, the type and amount of chemicals used in the treatment, and the number of times of treatment, are determined based on the detection results. As the conditions, the conditions that are usually used by those skilled in the art for each treatment can be used as they are or after being modified.

Sites where a large amount of amylase is detected are presumed to be sites where amylase or components containing amylase, such as saliva, tend to adhere in various forms. It is assumed that such a place is likely to be contaminated by, for example, saliva or viruses and/or bacteria taken into the saliva even after cleaning. This may be taken into account when determining the locations and conditions for sanitary treatment based on the amylase detection results.

In the present invention, amylase is detected at different locations in the target region, and sanitary treatment conditions can be determined from the frequency of amylase detection and the amount of amylase detected. Specifically, amylase is detected at different locations in the target region, and the sanitary treatment conditions can be determined from the amylase positive rate and the average amount of amylase calculated from the detection results.
Here, the amylase positive rate is the ratio of the number of sites where amylase was detected to the number of sites where amylase was detected, and is calculated by the following formula.
Amylase positive rate (%) = B / A × 100
A: number of locations where amylase was detected B: number of locations where amylase was detected The average amount of amylase is the average value of amylase amounts at locations where amylase was detected. In this case, the amount of amylase may be based on a relative indication of the detected amount, such as the detection intensity of the reagent.

The amylase positive rate may be interpreted as the probability that the target area is contaminated, and the average amylase amount may be interpreted as the average amount of contaminants attached to the target area. In the present invention, the possibility of viral and/or bacterial contamination can be determined based on the amylase detection results. In this case, the higher the amylase positive rate and the average amount of amylase, the higher the risk of infection when a person comes into contact with the surface of the target area.
In the present invention, it is preferable to set criteria for determining the possibility of viral and/or bacterial contamination from the amylase positive rate and the average amount of amylase, and determine the risk of infection based on the criteria. Specifically, the amylase positive rate and the average amount of amylase are each set at 2 stages or more, preferably 3 stages or more, and 10 stages or less, preferably 5 stages or less, and the infection risk is determined and sanitary treatment is performed. You can also determine the order of priority. For example, the amylase positive rate and the average amount of amylase may be correlated as shown in Table 1 below to perform risk determination and determine the order of priority for sanitary treatment. Note that the risk determination criteria in Table 1 are examples, and the magnitude of the influence of the amylase positive rate and the average amount of amylase on the infection risk may be determined based on criteria different from those in Table 1.

In the present invention, when amylase is detected at different locations in the target region, for example, ¹ location or more, 2 locations or more, 3 locations or more, 16 locations or less, 9 locations or less, and 6 locations per 1 m 2 Measurement can be performed on a plurality of points selected at a rate equal to or less than the number of points. The different locations may be selected in the same detection object or may be selected in different detection objects.

In the present invention, amylase can be detected by a method utilizing antigen-antibody reaction and/or enzymatic reaction. Detection of amylase in the target region may be performed, for example, directly on the surface of the detection target, or on a sample collected from the surface of the detection target. In the present invention, amylase is preferably detected by a method utilizing antigen-antibody reaction. In the present invention, for example, it is preferable to use a method using an antigen-antibody reaction that enables specific detection of human salivary amylase. Methods using antigen-antibody reaction include immunochromatography, enzyme immunoassay (EIA, ELISA), immunoprecipitation, immunoblotting and the like. A commercially available product that measures the amount of amylase using antigen-antibody reaction may be used.

In the present invention, the sanitary treatment may be a treatment applied to the target area where amylase was detected. That is, in the present invention, for example, when amylase is detected on the surface of a detection target, the sanitary treatment may be a sanitary treatment applied to the detection target and/or the environment containing the detection target.

In the present invention, the sanitary treatment may include a treatment for improving the sanitary condition of the target area, a treatment for maintaining the sanitary condition of the target area, and the like.
In general, the hygiene of the living environment is improved and/or maintained by keeping it clean. In the present invention, for example, sanitary treatment may include one or more treatments selected from cleaning, anti-bacterial treatment, and anti-adhesion treatment for viruses. More specifically, sanitary treatment may include, for example, one or more treatments selected from cleaning, bleaching, disinfecting, sterilizing, disinfecting, antibacterial, virucidal, antiviral, and virus removing. Each treatment can be performed by a known method.

According to the invention, a sanitary treatment may be a treatment that reduces the harm caused by viruses and/or bacteria. That is, the viruses and/or bacteria targeted by the present invention may be harmful viruses and/or harmful bacteria. Harm includes at least harm to humans. Harmful viruses may be non-enveloped or enveloped viruses, in particular enveloped viruses. Treatments that reduce the disadvantages posed by viruses and/or bacteria can in particular be selected from the treatments mentioned above. Disadvantages caused by viruses and/or bacteria are typically infections.

In the present invention, the sanitary treatment may be a treatment using a sanitary treatment agent (hereinafter referred to as a sanitary treatment agent) for at least one selected from cleaning, sterilization and virucidal.
The sanitary treatment agent can contain, for example, one or more selected from (a) surfactants, (b) oxidizing bleaches and (c) disinfectants. Sanitizing agents can contain water. The sanitary treatment agent may be either liquid or solid, preferably liquid, more preferably liquid containing water.

(a) Surfactants include one or more selected from anionic surfactants, nonionic surfactants, cationic surfactants, and amphoteric surfactants.
(a) Surfactants include, for example, linear alkylbenzenesulfonic acids and their salts, alkyl sulfates and their salts, polyoxyethylene alkyl ether sulfuric acids and their salts, fatty acids and their salts, polyoxyethylene alkyl ethers, and alkyl glycosides. , alkylamine oxides, quaternary ammonium salts, and alkylamine ethylene oxide adducts.
The straight-chain alkyl group of the straight-chain alkylbenzenesulfonic acid may have 8 or more and 20 or less carbon atoms.
The number of carbon atoms in the alkyl group of the alkyl sulfate ester may be 8 or more and 20 or less.
The polyoxyethylene alkyl ether sulfuric acid may have an average added mole number of ethylene oxide of 1 or more and 30 or less, and may have an alkyl group of 8 or more and 20 or less carbon atoms.
The fatty acid may have 8 or more and 20 or less carbon atoms. Salts include alkali metal salts such as sodium salts and potassium salts.
The polyoxyethylene alkyl ether may have an average added mole number of ethylene oxide of 1 or more and 30 or less, and may have an alkyl group of 8 or more and 20 or less carbon atoms.
The alkyl glycoside may have 8 or more and 20 or less carbon atoms in the alkyl group.
The alkylamine oxide may have an alkyl group having 8 or more and 20 or less carbon atoms.
The quaternary ammonium salts include benzalkonium chloride, benzethonium chloride, dialkyl chloride (having 8 to 20 carbon atoms) dimethylammonium and the like.
In the alkylamine ethylene oxide adduct, the alkyl group may have 8 or more and 20 or less carbon atoms, the average number of added moles of ethylene oxide may be 1 or more and 30 or less, and the amine may be primary, secondary or tertiary. can be Alkylamine ethylene oxide adducts include, for example, laurylamine ethylene oxide adducts.
Depending on the type of sanitary treatment, the sanitary treatment agent contains (a) a surfactant, for example, 0.0001% by mass or more, further 0.001% by mass or more, further 0.01% by mass, and further 0.1% by mass. 20% by mass or less, further 10% by mass or less, and further 5% by mass or less.

(b) Oxidative bleaching agents include hypochlorite, dichloroisocyanurate, hydrogen peroxide, sodium percarbonate and peracetic acid, with hypochlorite being preferred. Salts include alkali metal salts such as sodium salts and potassium salts. Although it depends on the type of sanitary treatment, the sanitary treatment agent contains (b) an oxidizing bleaching agent in an amount of, for example, 0.0001% by mass or more, further 0.001% by mass or more, further 0.01% by mass, furthermore 0.01% by mass or more. 1% by mass, further 1% by mass, 10% by mass or less, further 7.5% by mass or less, and further 5% by mass or less.

(c) The bactericidal agent includes one or more selected from bactericidal alcohols and bactericidal organic acids.

The bactericidal alcohol includes one or more selected from ethanol and propanol. Depending on the type of sanitary treatment, the sanitary treatment agent contains, for example, 0.1% by mass or more, further 1% by mass or more, further 10% by mass, further 40% by mass, and 95% by mass or less. , 90% by mass or less, and further 85% by mass or less.

Bactericidal organic acids include lactic acid or salts thereof. Salts include alkali metal salts such as sodium salts and potassium salts. Depending on the type of sanitary treatment, the sanitary treatment agent contains a bactericidal organic acid, for example, 0.01% by mass or more, further 0.1% by mass or more, further 0.4% by mass, and 7% by mass or less. , 5% by mass or less, and further 3% by mass or less.

(c) The bactericide may be one or more selected from ethanol, propanol, lactic acid and lactic acid salts.

The sanitary treatment agent preferably contains two or more selected from (a) a surfactant, (b) an oxidizing bleach and (c) a disinfectant.

In the present invention, the sanitary treatment may include contacting the treated area with a sanitary treatment agent and wiping the treated area contacted with the sanitary treatment agent with a flexible absorbent material. In the present invention, the contact of the sanitary treatment agent with the treatment area is at least contact of a flexible absorbent material impregnated with the sanitary treatment agent with the treatment area and spraying of the sanitary treatment agent on the treatment area. It can be done by one side. For example, by wiping a treated area with a flexible absorbent material impregnated with a sanitary treatment agent, the treated area can be wiped while the sanitary treatment agent is in contact with the treated area. Spraying can be performed using, for example, a trigger-type pump. Non-woven fabrics, woven fabrics, sponges and the like can be used as flexible absorbent materials.

INDUSTRIAL APPLICABILITY The present invention provides a sanitary treatment method determination method for detecting amylase in the environment, and determining a location for sanitary treatment and sanitary treatment conditions based on the detection results.
In this method, the method of determining the location to be sanitized may be the method of determining the location of sanitary treatment of the present invention.
Also, in this method, the method for determining the sanitary treatment conditions may be the method for determining the sanitary treatment conditions of the present invention.
The sanitary treatment method is determined based on the location to be sanitized and the sanitary treatment conditions thus determined.

In the present invention, amylase detection is performed to determine sanitization sites and/or sanitization conditions. In the present invention, amylase detection may be performed each time sanitary treatment is performed, but it is not necessary to perform it every time. In addition, for example, based on the information of the location and / or conditions determined by the present invention, if the processing method such as the type, degree, frequency of sanitary treatment for each location is manualized, after that, the location As long as the application does not change significantly, highly hygienic treatment can be continuously performed without necessarily detecting amylase. In addition, once the site to be sanitized and/or sanitary treatment conditions are determined, amylase detection may be performed periodically or irregularly to confirm the quality and effectiveness of the sanitary treatment method. do not have.

The determination method of the present invention can be used to assist in sanitary treatment, for example, for the above-mentioned buildings, public transport zones and their fixtures. That is, information on the sanitary treatment method determined by the present invention can be provided to the person who performs the sanitary treatment, and the prescribed sanitary treatment can be performed reliably and efficiently.

As a more specific aspect of the present invention, amylase is detected in the target region, the location where amylase is detected is determined as a location that may be contaminated with viruses and / or bacteria, and based on the amylase detection result , a sanitary treatment method determining method for determining the sanitary treatment conditions to be applied to the determined location. To this method, the items described in each determination method of the present invention can be appropriately applied.
As another more specific aspect of the present invention, amylase is detected in a building, for example, indoors and/or outdoors. and determining conditions for sanitary treatment to be applied to the determined site based on the amylase detection result. To this method, the items described in each determination method of the present invention can be appropriately applied.

According to the present invention, there is provided a sanitary treatment method in which at least one of a location to be sanitized and conditions for sanitary treatment are determined by the determination method of the present invention, and sanitary treatment is performed based on the determination.
That is, the sanitary treatment method of the present invention is
(1) By the determination method of the present invention, the location to be sanitized is determined, and the determined location is sanitized.
(2) The conditions for sanitary treatment are determined by the determination method of the present invention, and sanitary treatment is performed under the determined conditions, or (3) The location to be sanitized and the conditions for sanitary treatment are determined by the determination method of the present invention. determine and sanitize the determined areas under the determined conditions;
It is a sanitary treatment method.
To the sanitary treatment method of the present invention, the items described in the determination method of the present invention can be appropriately applied. In the sanitary treatment method of the present invention, the sanitary treatment is also preferably performed using a sanitary treatment agent. Specific examples and preferred examples of the sanitary treatment and sanitary treatment agent can also be applied to those described in the determination method of the present invention.

INDUSTRIAL APPLICABILITY The present invention provides an estimation method for detecting amylase in the environment and estimating locations where harmful viruses and/or harmful bacteria are likely to exist based on the detection results.
In addition, according to the present invention, amylase is detected in the environment, based on the detection results, the location where the harmful virus and/or harmful bacteria are likely to exist is estimated, and the infection route, that is, the harmful virus and/or the harmful An estimation method is provided for estimating infection routes by fungi. The route of infection here may be a route of infection to humans. In addition, the route of infection may be a route for an infection that has already occurred or a route for an infection that is expected to occur in the future.
In the estimation method of the present invention, even in the first environment, harmful viruses and / or harmful bacteria may exist through empirical knowledge or artificial intelligence (AI) as past data is accumulated. It also includes estimating high risk sites or infection routes. Alternatively, the amylase may be detected after presuming it.
To these methods, the items described in each determination method of the present invention can be appropriately applied.
Also in these methods, the harmful virus may be a non-enveloped or enveloped virus, particularly an enveloped virus.

Harmful bacteria include, for example, Pneumococcus. Examples of non-enveloped viruses include adenovirus, rhinovirus and norovirus. Examples of enveloped viruses include SARS-CoV-2, influenza, SARS, MARS and respiratory syncytial virus.

The present invention provides a sanitary treatment kit comprising means for detecting amylase in a target region and agents used for sanitizing sites determined by the amylase detection results obtained by the means. Means for measuring the amount of amylase include, for example, immunochromatography, enzyme immunoassay (EIA method, ELISA method), immunoprecipitation method, immunoblotting, enzyme activity measurement and the like. Examples of the drug include detergents, bleaches, disinfectants, viricides, antiviral agents, disinfectants, and antibacterial agents.

According to the present invention, a method for displaying a contamination situation at a candidate site for sanitary treatment, comprising:
(a) identifying one or more locations in the environment, comprising steps (i)-(iii) below;
(i) generating location data capable of identifying the location of the entity to identify the one or more locations within the defined area;
(ii) recording location data for said one or more locations within said environment; a step of separately recording;
(b) recording amylase detection results from one or more locations within the environment;
(c) recording the generated detection result for each segmented position and part of the physical entity recorded in (iii) of (a); and (d) recording the one or more records of (c) in the environment. a step of displaying together with the position data of
A method for displaying contamination status at a candidate site for sanitary treatment is provided, comprising:
The display method of the contamination status of the present invention is preferably performed by an information processing device (PC, smart phone, etc.). That is, it is preferable that each data in each step is recorded by an information processing apparatus. FIG. 1 shows an outline of the procedure of the method for displaying the state of contamination at candidate sites for sanitary treatment according to the present invention. In FIG. 1, ST stands for step. In FIG. 1, step (a) (not shown) is executed by ST(a)-(i), ST(a)-(ii), and ST(a)-(iii).

Step (a) is a step of identifying one or more locations in the environment and appropriately selecting candidate locations for sanitary treatment in the environment. For example, items in the room and items in the toilet can be selected as the location.
Step (i) [ST(a)-(i) in FIG. 1] generates location data capable of identifying the installation location of the entity to identify the one or more locations within the defined area. is a step. The area may be, for example, a predetermined area in a room. Examples of the actual objects include items in a room and items in a toilet. The position data is, for example, data indicating the indoor positions of those articles.
Step (ii) [ST(a)-(ii) in FIG. 1] is a step of recording position data of the one or more locations in the environment.
Step (iii) [ST(a)-(iii) in FIG. 1] is a step of recording the actual entity linked to the location data of the one or more locations in the environment by classifying the locations and locations. is. For example, when there are a plurality of articles with the same attribute in a room, they are sorted and information is created and recorded for each of them. Specifically, when there are two chairs in the room, the chairs are sorted into chairs A and B, and the position data of each is recorded. Furthermore, if each chair has two armrests, position data can be created by subdividing the chair into armrests A1 and A2. Of course, this processing method can be applied to other entities (such as articles).

Step (b) [ST(b) in FIG. 1] is a step of recording the amylase detection results detected from one or more locations in the environment. Amylase detection is performed at the location and site of the entity fractionated in step (iii) of step (a). The detection result can be obtained, for example, by the detection method employed in the sanitary treatment site determination method of the present invention.

Step (c) [ST(c) in FIG. 1] records the generated detection results by linking them with the separated positions and parts of the actual objects recorded in step (iii) of step (a). is a step. By this step (c), the detection result for the actual object at the predetermined position and the detection result for the predetermined portion of the actual object at the predetermined position are recorded, and the position and portion of the actual object and the detection result are linked.

Step (d) [ST(d) in FIG. 1] is the step of displaying the record of step (c) together with location data for said one or more locations within said environment. The display is performed, for example, on an information display terminal such as an external monitor for a personal computer or a smartphone screen.

For example, in FIG. 1, with ST(a)-(i) to ST(d) as one unit, ST(a)-(i) to ST(d) are repeated until a predetermined number of times. , it is also possible to terminate after reaching a predetermined number of times.
Further, for example, in FIG. 1, ST(a)-(i) to ST(d) are set as one unit, and ST(a)-(i) to ST(d) are repeatedly performed at predetermined intervals. , can be terminated after reaching a predetermined time.

According to the present invention, there is provided a method for displaying sanitary treatment locations, which determines sanitary treatment locations based on the contamination status of candidate sanitary treatment locations displayed by the display method of the present invention and displays them as sanitary treatment locations. It is preferable that the display method of the sanitary treatment area of the present invention is performed by an information processing device (PC, smart phone, etc.). In the sanitary treatment location display method of the present invention, one or more locations in the environment specified in step (a) are sanitized based on data of a plurality of candidate locations for sanitary treatment acquired at different measurement times. Processing locations can be determined. AI, for example, can be used to determine sanitary treatment locations. That is, for example, data such as amylase detection results, locations and articles where amylase was detected, past occurrences of infection, etc. can be accumulated, and AI can be used to determine sanitary treatment locations.

The method of displaying candidate locations for sanitary treatment and/or the method of displaying sanitary treatment locations of the present invention is incorporated into the method of determining sanitary treatment locations, the method of determining sanitary treatment conditions, the method of determining sanitary treatment methods, and the estimation method of the present invention. can be implemented in For example, in the sanitary processing location display method of the present invention, the possibility of contamination by viruses and/or bacteria is determined based on the contamination status in the contamination status display method of the present invention, and the sanitary processing location is determined from the determination result. can do. Determining the likelihood of contamination may include estimating the likelihood of contamination.
It should be noted that the results displayed by the present invention can be printed out on a paper medium or the like as necessary. Also, the electronic data of the displayed results can be saved in a suitable storage medium. In addition, the electronic data of the displayed results are used secondarily using an information communication network (Internet), for example, creating a sanitary treatment implementation plan, sanitary treatment procedure guidance based on the implementation plan, etc. can be used for services.

The following are examples of aspects of the present invention. These aspects include the sanitary treatment location determination method, sanitary treatment condition determination method, sanitary treatment method determination method, sanitary treatment method, sanitary treatment kit, estimation method, and contamination status at candidate sanitary treatment locations. Items described in the method of labeling and method of labeling sanitary treatment areas can be applied as appropriate.

<1>
A method for determining sanitary treatment sites, comprising detecting amylase in the environment and determining sites to be sanitized based on the detection results.

<2>
The determination method according to <1>, wherein amylase is detected at different locations in the environment, and the location where amylase is relatively detected in the detected location is the location where sanitary treatment is performed.

<3>
The method for determining a location to be sanitized according to <1> or <2>, wherein the possibility of contamination with viruses and/or bacteria is determined based on the amylase detection result, and the location to be sanitized is determined from the determination result. .

<4>
A method for determining sanitary treatment conditions, comprising detecting amylase in the environment and determining sanitary treatment conditions based on the detection results.

<5>
The determination method according to <4>, wherein amylase is detected at different locations in the environment, and the sanitary treatment conditions are determined from the frequency of amylase detection and the amount of amylase detected.

<6>
The determination method according to <4> or <5>, wherein the possibility of contamination with viruses and/or bacteria is determined based on the amylase detection results, and the sanitary treatment conditions are determined from the determination results.

<7>
A method for determining a sanitary treatment method, comprising detecting amylase in the environment, and determining a location to be sanitized and sanitary treatment conditions based on the detection results.

<8>
The determination method according to <7>, wherein amylase is detected at different locations in the environment, and the location where amylase is relatively detected among the locations where detection is performed is the location where sanitary treatment is performed.

<9>
The determination method according to <7> or <8>, wherein amylase is detected at different locations in the environment, and sanitary treatment conditions are determined from the frequency of amylase detection and the amount of amylase detected.

<10>
Any of <7> to <9>, based on the amylase detection results, determining the possibility of contamination by viruses and/or bacteria, and determining the location and/or conditions for sanitary treatment from the determination results. or the determination method according to item 1.

<11>
The determination method according to any one of <1> to <10>, wherein the sanitary treatment is treatment to reduce disadvantages caused by viruses and/or bacteria.

<12>
The determination method according to any one of <1> to <10>, wherein the sanitary treatment includes one or more treatments selected from purification, anti-adhesion treatment for bacteria, and anti-adhesion treatment for viruses.

<13>
The determination method according to any one of <1> to <12>, wherein amylase is detected by a method utilizing antigen-antibody reaction and/or enzymatic reaction.

<14>
The determination method according to any one of <1> to <13>, wherein amylase is detected by a method utilizing an antigen-antibody reaction.

<15>
The determination method according to any one of <1> to <14>, wherein the amylase is a human-derived amylase.

<16>
The determination method according to any one of <1> to <15>, wherein the environment is a living environment.

<17>
The determination method according to any one of <1> to <16>, wherein the environment includes an object for detecting amylase (hereinafter referred to as a detection object), and amylase is detected on the surface of the detection object. .

<18>
The determination method according to <17>, wherein the detection target includes a detection target selected from buildings, building fixtures, public transportation, and public transportation fixtures.

<19>
The building is a selected commercial facility, accommodation facility, educational facility, medical facility, nursing care facility, business facility, and eating and drinking facility, and the public transportation facility is selected from rail vehicles, road vehicles, ships, and aircraft. The determination method according to <18>, which is public transportation.

<20>
Any one of <17> to <19>, wherein the detection target includes a detection target selected from tables, chairs, sinks, toilet seats, doorknobs, faucets, switches, remote control devices, and computer input devices. Determination method described.

<21>
The determination method according to any one of <1> to <20>, wherein the sanitary treatment is treatment using a sanitary treatment agent for at least one selected from cleaning, sterilization and virucidal.

<22>
The determination method according to <21>, wherein the sanitary treatment agent contains one or more selected from (a) a surfactant, (b) an oxidizing bleach and (c) a disinfectant.

<23>
The determination method according to <21> or <22>, wherein the sanitary treatment agent contains two or more selected from (a) a surfactant, (b) an oxidizing bleach and (c) a disinfectant.

<24>
(a) the surfactant is selected from linear alkylbenzene sulfonic acids and their salts, fatty acids and their salts, polyoxyethylene alkyl ethers, alkyl glycosides, alkylamine oxides, quaternary ammonium salts, and alkylamine ethylene oxide adducts1 The determination method according to <22> or <23>, which is at least a species.

<25>
(b) The determination method according to any one of <22> to <24>, wherein the oxidative bleaching agent is hypochlorite.

<26>
(c) The determination method according to any one of <22> to <25>, wherein the germicidal agent is one or more selected from germicidal alcohols and germicidal organic acids.

<27>
(c) The determination method according to any one of <22> to <26>, wherein the disinfectant is one or more selected from ethanol, propanol, lactic acid, and lactic acid salts.

<28>
Any one of <21> to <27>, wherein the sanitary treatment includes contacting the treated area with the sanitary treatment agent and wiping the treated area contacted with the sanitary treatment agent with a flexible absorbent material. The determination method according to item 1.

<29>
The sanitary treatment agent is brought into contact with the treated area by at least one of contacting the treated area with a flexible absorbent material impregnated with the sanitary treatment agent and spraying the sanitary treatment agent on the treated area. , the determination method described in <28>.

<30>
A sanitary treatment method, comprising: determining at least one of the location to be sanitized and the sanitary treatment conditions by the determination method according to any one of <1> to <29>, and performing the sanitary treatment based on the determination.

<31>
The sanitary treatment method according to <30>, wherein the sanitary treatment is treatment using a sanitary treatment agent for at least one selected from cleaning, sterilization, and virucidal.

<32>
The sanitary treatment method according to <31>, wherein the sanitary treatment agent contains one or more selected from (a) a surfactant, (b) an oxidizing bleach and (c) a bactericide.

<33>
The sanitary treatment method according to <31> or <32>, wherein the sanitary treatment agent contains two or more selected from (a) a surfactant, (b) an oxidizing bleach and (c) a disinfectant.

<34>
(a) the surfactant is selected from linear alkylbenzene sulfonic acids and their salts, fatty acids and their salts, polyoxyethylene alkyl ethers, alkyl glycosides, alkylamine oxides, quaternary ammonium salts, and alkylamine ethylene oxide adducts1 The sanitary treatment method according to <32> or <33>, which is at least seed.

<35>
(b) The sanitary treatment method according to any one of <32> to <34>, wherein the oxidative bleaching agent is hypochlorite.

<36>
(c) The sanitary treatment method according to any one of <32> to <35>, wherein the bactericidal agent is one or more selected from bactericidal alcohols and bactericidal organic acids.

<37>
(c) The sanitary treatment method according to any one of <32> to <36>, wherein the disinfectant is one or more selected from ethanol, propanol, lactic acid, and lactic acid salts.

<38>
A sanitary treatment kit, comprising: means for detecting amylase in the environment; and an agent used for treating a sanitized site determined by the amylase detection result obtained by said means.

<39>
An estimation method comprising detecting amylase in the environment and estimating locations where harmful viruses and/or harmful bacteria are likely to exist based on the detection results.

<40>
An estimation method comprising detecting amylase in the environment, estimating locations where harmful viruses and/or harmful bacteria are likely to exist based on the detection results, and estimating infection routes.

<41>
The estimation method according to <39> or <40>, wherein the harmful virus is an enveloped virus.

<42>
A method for displaying a contamination status at a candidate site for sanitary treatment, comprising:
(a) identifying one or more locations in the environment, comprising steps (i)-(iii) below;
(i) generating location data capable of identifying the location of the entity to identify the one or more locations within the defined area;
(ii) recording location data for said one or more locations within said environment; a step of separately recording;
(b) recording amylase detection results from one or more locations within the environment;
(c) recording the generated detection result for each segmented position and part of the physical entity recorded in (iii) of (a); and (d) recording the one or more records of (c) in the environment. a step of displaying together with the position data of
A method for displaying the contamination status at a candidate site for sanitary treatment, comprising:

<43>
A method of displaying sanitary treatment locations, wherein a sanitary treatment location is determined based on the contamination status of the sanitary treatment candidate location displayed by the display method <42> and displayed as a sanitary treatment location.

<44>
The method for displaying sanitary treatment locations according to <43>, wherein the possibility of contamination with viruses and/or bacteria is determined based on the contamination status, and the sanitary treatment locations are determined from the determination result.

<Example 1 and Comparative Example 1>
(1) Example 1
Contamination risk was determined for commercial facilities (shopping centers).
A commercial facility was divided into three areas: (1) a sales floor area, (2) a dining area, and (3) a rest area, and amylase was detected at inspection points in each area. From the detection results, the average amount of amylase and the amylase positive rate were determined for each area, and the degree of contamination risk was determined based on Table 1 below. Table 2 shows the results.

At each test site, a 25 cm ² area was swabbed with a clean cotton swab. Amylase was detected from the swabbed cotton swab using an immunochromatographic kit (Independent Forensics, RSID (registered trademark) SALIVA [model number: 0100]), and negative "-" was given when amylase was not detected at the target test site. and positive "+" when detected. In the case of positive, the amylase amount was evaluated by visual observation in 3 grades according to the intensity of the band on the test line. When the band was barely confirmed, it was rated as +1, when it was clearly confirmed, it was rated as +2, and when it was strongly confirmed, it was rated as +3. It can be judged that the larger the positive value, the larger the amount of attached amylase.
When amylase solutions with known concentrations were measured, the amount of amylase corresponding to ^each determination result was "-" for less than 10 ng, "+1" for 10 or more and less than 25 ng, and "+2" for 25 or more. Less than 60 ng, "+3" was 60 ng or more.

In addition, the above test method can be implemented in a manual, and a kit suitable for the contents of the manual, for example, a kit suitable for implementation under the following conditions (hereinafter referred to as manual example 1) can be used. .
Wetting liquid: 0.1 ml of an aqueous solution of pH 7 containing 0.05% Tween 80 in distilled water was sealed in a 0.5 ml microtube. A cotton swab of 75 mm in length and 30 mg in weight Extract solution: 0.1 ml of an aqueous solution of pH 7 containing 0.05% Tween 80 in distilled water, sealed in a squeezable tube. to absorb the wetting liquid.

In the case of the procedure under the above conditions, a predetermined area, for example, an area of 50 mm × 50 mm is selected from the surface of the object to be inspected, for example, the surface of the object, and it is vertically, horizontally, diagonally right, and diagonally left (the order of vertical, horizontal, and diagonal does not matter). Bring the wetted collections into contact. It is preferable to make contact so that the surface of the cotton swab touches evenly. contact so as not to
The carrier, such as the collected swab, may be placed directly into a suitable container, such as a plastic container with a zipper or a sample bottle, and the container may contain the extract. Extraction of amylase (acquisition of liquid containing amylase) and detection and quantification of amylase are carried out from the carrying part such as the collected cotton swab by the following methods. After collection, the sample may be moved to another location and the extraction operation may be performed in the take-home workroom.
Extraction method: The sampling part of a cotton swab was soaked in the extract, and the cotton ball was rubbed from above the tube.
Detection method: For example, a commercially available immunochromatography kit (IndependentForensics, RSID (registered trademark) SALIVA [model number: 0100]) is mixed with 0.08 mL of the attached Running buffer and 0.02 mL of the extract and added dropwise to detect amylase. .
Quantification method: Amylase can also be quantified from the density of the band at the detection site by reducing the error in the collection method described above. Serial dilutions of amylase solutions of known concentrations may be measured, and the density of the bands may be visually determined using a color scale, or may be quantified using a commercially available immunochromatographic reader.
The collection method may be set so that it can be easily treated with a commercially available amylase assay kit.

(2) Comparative Example 1
The amount of ATP (RLU) was measured at the test points in each area of the same commercial facility as in Example 1. From the measurement results, the average ATP amount was obtained for each area. Table 2 shows the results. The amount of ATP was measured by wiping a target area of about 25 cm ² with a LuciPac Pen manufactured by Kikkoman Biochemifa, and using Lumitester Smart manufactured by Kikkoman Biochemifa according to the method attached to the product.

The average ATP amount of Comparative Example 1 includes not only viruses and/or bacteria, but also other organic matter such as dirt. On the other hand, as shown in Reference Example 3 below, it is suggested that there is a correlation between the amount of amylase and the amount of virus. Therefore, it can be judged that the contamination risk degree based on the amylase detection result in Example 1 is more reliable than the contamination risk degree based on the average ATP amount in Comparative Example 1.
In Example 1, amylase-contaminated sites, that is, sites with high possibility of being contaminated with components containing amylase, could be estimated by measuring the amount of amylase. This could not be estimated from the amount of ATP. For example, in Comparative Example 1 in Table 2, the ATP amount of 18,860 RLU in the floor (1) was greater than the 17,973 RLU ATP amount in the handle (1) of the shopping basket, but in Example 1, the amylase amount was greater in the former.
Based on the results of Example 1, sanitary treatment of environmental surface contamination by amylase and components containing amylase can be carried out only at locations where amylase is detected, and the work can be made more efficient. In addition, based on the results of Example 1, it is possible to estimate locations where harmful viruses and/or harmful bacteria are likely to exist, and further estimate routes of infection to humans.
In Example 1, areas were set such as a sales floor area, a dining area, and a rest area, and the degree of contamination risk was evaluated based on the amylase detection results in each area. As a result, the eating area and rest area had a higher degree of contamination risk than the sales area. Therefore, it can be proposed to allocate more labor (materials and personnel) for sanitary treatment to the dining area and rest area. When the average ATP amount for each area is calculated as in Comparative Example 1, the value in the sales floor area is larger than that in the rest area, which is different from the trend in Example 1.

<Example 2 and Comparative Example 2>
(1) Example 2
Contamination risk was determined for hotel guest rooms.
For 8 guest rooms of the same hotel, the amount of amylase was measured at the inspection points shown in Table 3 in the same manner as in Example 1, and the average value of the 8 rooms was determined as the average amount of amylase. Also, from the measurement results, the amylase positive rate was obtained, and the degree of contamination risk was determined based on Table 1 above. Table 3 shows the results. An example of displaying the contamination status based on the results of Example 2 is schematically shown in FIG. FIG. 2 schematically shows an outline of the hotel guest room and the inspection points, and the black circle attached to the inspection points indicates the average amount of amylase. The size of the black circle correlates with the average amount of amylase, and the larger the circle, the higher the average amount of amylase.

(2) Comparative Example 2
The ATP amount (RLU) was measured in the same manner as in Comparative Example 1 at the test points in the eight guest rooms of the same hotel as in Example 2. Based on the measurement results, the average ATP amount was obtained for each test site. Table 3 shows the results.

Based on the contamination risk determination in Example 2, it was possible to propose the ranking of places where careful sanitary treatment should be performed or places where sanitary treatment should be preferentially performed. On the other hand, in Comparative Example 2, for example, the amount of ATP at the edge of the washbasin sink, which has the highest degree of contamination risk, is the lowest in Example 2, indicating a different tendency from Example 2. Further, based on the results of Example 2, it is possible to estimate locations where harmful viruses and/or harmful bacteria are likely to exist, and further estimate routes of infection to humans.
The contamination status in FIG. 2 can be displayed on an output device of an information processing device (PC, smart phone, etc.). By displaying the contamination status as shown in FIG. 2, the relationship between the inspection location and the contamination status can be visually grasped easily. Furthermore, it is possible to determine a sanitary treatment location based on the contamination status displayed in FIG. 2 and display it as a sanitary treatment location.

<Reference example 1>
The table of the commercial facility where amylase was detected in Example 1 (inspection area: meal area, table different from inspection points (1), (2), and (3)) was used as a test subject, and the sanitary treatment effect was evaluated. carried out.
The table is covered with a cloth moistened with tap water, or a cloth dampened with a commercially available medical detergent (Medical Clean Keeper, Kao Corporation) diluted 200 times with tap water. Sanitization was performed by wiping once in each direction. Wipes with tap water dampened wipes and commercial medical detergent wipes were performed on separate tables.
Before and after the sanitary treatment, the amount of amylase was measured in the same manner as in Example 1, and the amount of ATP was measured in the same manner as in Comparative Example 1. Table 4 shows the results.

From the results in Table 4, it was determined that wiping with a medical cleaning agent was more effective in reducing the amount of amylase than wiping with water in the equipment of the facility.

<Reference example 2>
Amylase was detected in the following cases: (1) sneezing indoors, (2) three people eating at a table indoors, and (3) web conference using a computer indoors. .
In (1), one subject stood up in a room with the windows and doors closed, and sneezed five times without wearing a mask. detected.
In (2), three subjects sat at a table for four people (without partitions), had a meal for 30 minutes while talking without a mask, and detected amylase between two people in the middle of the table and side by side. gone.
In (3), one subject sat at a desk, held a web conference for 2 hours and 30 minutes using a personal computer with a mask, and detected amylase on the keyboard of the personal computer.
Amylase was detected in the same manner as in Example 1. Locations where a large amount of amylase was detected are locations with a high probability of having stains derived from saliva. Table 5 shows the results.

How the saliva droplets scatter under the conditions (1) to (3) above is predicted by, for example, simulations using the supercomputer "Fugaku." The results in Table 5 were confirmed to correlate with such expected results. Therefore, it is highly likely that the locations where amylase was detected in Examples 1 and 2 were contaminated with droplets of saliva expelled from the human mouth.

<Reference example 3>
A bacteriophage MS2 solution (10 ¹² PFU/mL) was mixed with saliva so that the bacteriophage MS2 concentration was 10 ¹⁰ PFU/mL. 0.1 mL of this liquid was measured, applied to a 4 cm×4 cm area on a PP (polypropylene) plate, and allowed to stand for 1 hour in a safety cabinet to dry. This was defined as the initial adhesion point. The following operations were performed from the initial attachment point.
A clean finger was lightly rubbed once on an arbitrary portion of the initial adhered portion, and the new uncontaminated PP plate was lightly rubbed once with the finger. This operation was repeated 5 times. An uncontaminated PP plate was rubbed within an area of 4 cm x 4 cm. A scratched portion on a new PP plate was defined as a propagated portion (1). The same operation was performed from the propagation point (1) to another new PP plate, which was designated as the propagation point (2). Further, the same operation was performed from the propagation point (2) to another new PP plate, which was designated as the propagation point (3). The site of initial attachment and the sites of propagation (1) to (3) were thoroughly wiped with a sterile cotton swab moistened with sterile water. A cotton swab was dipped in 0.2 mL of an extraction buffer (RSID (registered trademark) SALIVA Extraction Buffer) attached to the kit to prepare an extract of the swabbed components. Of the extract, 0.1 mL was used to measure the amount of virus by plaque assay described later, and the rest was used to measure the amount of amylase using an immunochromatographic kit (Independent Forensics, RSID (registered trademark) SALIVA [model number: 0100]). . Table 5 shows the results.

[Method of plaque assay]
LB agar medium (agar concentration: 1.5%) supplemented with calcium chloride to a final concentration of 2.25 mM is sterilized with high pressure steam, and 15 mL is placed in a petri dish (Nissui Pharmaceutical Co., Ltd., Nissui sterilized shallow petri dish [model number: 06300]) and allowed to stand at room temperature to solidify. LB agar medium (agar concentration 0.6%) added with calcium chloride to a final concentration of 2.25 mM was sterilized with high pressure and then kept at about 40°C. ) was mixed with 0.1 mL of the above extract, and the prepared LB agar medium was poured into a hardened petri dish and laminated. This was statically cultured overnight at 37° C., and the number of lysed portions (plaques) infected with E. coli virus on the agar was counted as the amount of virus (PFU/cm ² ).

By the method of this reference example, it was possible to confirm the transmission of saliva by hand contact. Therefore, it was suggested that the sites where amylase was detected in Examples 1 and 2 may be not only sites where droplets adhere but also sites where saliva propagates through contact with an object such as a hand. In addition, since it can be confirmed that the tendency of the change due to the transmission of the amount of virus and the amount of amylase in this reference example is similar, the place where amylase was detected in the actual environment is the attachment mode of amylase (due to adhesion of droplets or contact transmission), there is a risk of contamination with amylase-containing components such as saliva and thus viruses and/or bacteria in saliva. This result also suggests that the amylase detection results can be used to estimate infection routes by viruses and/or bacteria.

<Reference example 4>
5 μL of 20-fold diluted saliva (collected from a 30-year-old man) was coated on a polypropylene substrate and dried to prepare a plurality of substrates. Amylase was measured from each substrate according to Manual Example 1 described above. However, the swab, which is the sampling part, has (1) a wooden support (manufactured by Japan Cotton Swab Co., Ltd., cotton swab with tester CTB-15), and (2) a support made of resin (manufactured by Eiken Chemical Co., Ltd. , γ correct swab R, TC2400) was used. In addition, the amylase was quantified in (I) a group that was quantified immediately after collection, (II) a group that was quantified after storing the cotton swab after collection at 4 ° C. for 4 or 10 days, and (III) after collection. Swabs were stored at 25° C. for 4 or 10 days before being divided into groups for quantification. Storage was carried out in testers attached to each swab. The amount of amylase was quantified by the same method as in Example 1. Table 7 shows the results.

From Table 7, it can be seen that when a cotton swab with a wooden support is used, storage at 25°C lowers the detection sensitivity. When the support was made of wood, the detection intensity was maintained for 4 days by storage at 4°C, but the detection sensitivity decreased after 10 days. It turns out to be preferable. On the other hand, when a cotton swab with a resin support was used, the detection sensitivity was maintained for 4 days even at 25°C. It can be seen that resin is preferable to wooden.

<Reference example 5>
A plurality of substrates to which saliva was adhered were prepared in the same manner as in Reference Example 4, and amylase was quantified from each substrate in the same manner as in Reference Example 4. However, a cotton swab with a wooden support (manufactured by Nippon Cotton Swab Co., Ltd., cotton swab with tester CTB-15) was used. In addition, amylase is quantified by extracting immediately after collecting with a cotton swab, (i) the extract is stored at 4 ° C. for 4 days or 10 days, and then quantified. After storage at 25° C. for 4 days or 10 days, the samples were divided into groups for quantification. Table 8 shows the results.

From Table 8, it can be seen that when the extract collected from the cotton swab is stored before amylase quantification, the detection sensitivity is maintained at both 4°C and 25°C for 4 days. In addition, since low-temperature storage maintained the detection sensitivity for a long period of time, it can be seen that low-temperature storage is preferable when the step of storing the extract is included.

<Formulation example>
Tables 9 to 11 show formulation examples of sanitary treatment agents that can be used for sanitary treatment according to the present invention. The sanitary treatment method of the present invention can be carried out using these sanitary treatment agents. However, sanitary treatment agents are not limited to these.
Table 9 shows treatment agents that are mainly used by impregnating sheet materials such as non-woven fabrics, and can be used to obtain sheet-shaped sanitary treatment agent products. A sheet-shaped sanitary treatment product is suitable for contacting and wiping the treatment area with the sanitary treatment agent.
Table 10 shows treatment agents that are mainly sprayed and used, and can be used to obtain spray-type sanitary treatment agent products. A spray-type sanitary treatment product is suitable for efficiently bringing the sanitary treatment agent into contact (spraying) with the treatment site. After contacting the sanitary treatment agent with a spray-type sanitary treatment product, if necessary, the treated area may be wiped with a flexible material such as a nonwoven fabric or a sponge.
Table 11 shows treatment agents mainly used for cleaning and sterilization purposes, and products can take various forms. For example, it can be used for sheet-like sanitary treatment agent products and spray-type sanitary treatment agent products as described above.

Components (part) used in Tables 9 to 11 are as follows.
Amphithol 20N: manufactured by Kao Corporation, lauryl dimethylamine oxide Amphithol 20AB: manufactured by Kao Corporation, lauramidopropyl betaine Neoperex GS: manufactured by Kao Corporation, alkylbenzene sulfonic acid Emar 20CS: manufactured by Kao Corporation , Sodium polyoxyethylene lauryl ether sulfate, secondary alcohol EO 3 moles: a nonionic surfactant in which an average of 3 moles of ethylene oxide is added to a secondary alcohol (12 to 14 carbon atoms), Softanol 30, Nippon Shokubai Co., Ltd., alkyl glycoside : AG-124, manufactured by Kao Corporation, nonionic surfactant Rhodol TW-L120: manufactured by Kao Corporation, polyoxyethylene sorbitan monolaurate/laurylamine EO adduct (2 mol): laurylamine with ethylene oxide Nonionic surfactant added with an average of 2 moles ・Penetol GE-EH: manufactured by Kao Corporation, 2-ethylhexylglyceryl ether ・Sanisol B-50: manufactured by Kao Corporation, alkylbenzyldimethylammonium chloride ・BDG: butyl diglycol ( Diethylene glycol monobutyl ether) Nippon Nyukazai Co., Ltd., solvent/MFG: propylene glycol monomethyl ether, Nippon Nyukazai Co., Ltd., solvent/betaine polymer: Example of JP 2018-199770, co-produced by the method of Production Example 1 Polymer/Carbopol ETD2020 PLYMER: manufactured by Lubrizol Advanced Materials, carboxyvinyl polymer/preservative: Kayson CG, manufactured by DuPont

Claims (22)

A method for determining sanitary treatment sites, comprising detecting amylase in the environment and determining sites to be sanitized based on the detection results. 2. The method for determining sanitary treatment sites according to claim 1, wherein the possibility of contamination by viruses and/or bacteria is determined based on amylase detection results, and the sites to be sanitized are determined from the determination results. A method for determining sanitary treatment conditions, comprising detecting amylase in the environment and determining sanitary treatment conditions based on the detection results. 4. The determination method according to claim 3, wherein the possibility of contamination by viruses and/or bacteria is determined based on the amylase detection results, and the sanitary treatment conditions are determined from the determination results. A method for determining a sanitary treatment method, comprising detecting amylase in the environment, and determining a location to be sanitized and sanitary treatment conditions based on the detection results. The determination method according to claim 5, wherein the possibility of contamination by viruses and/or bacteria is determined based on the amylase detection results, and the location and/or conditions for sanitary treatment are determined from the determination results. The determination method according to any one of claims 1 to 6, wherein the sanitary treatment includes one or more treatments selected from cleaning, anti-adhesion treatment for bacteria, and anti-adhesion treatment for viruses. The determination method according to any one of claims 1 to 7, wherein amylase is detected by a method utilizing antigen-antibody reaction and/or enzymatic reaction. A determination method according to any one of claims 1 to 8, wherein the environment is a living environment. The determination method according to any one of claims 1 to 9, wherein the environment includes an object for detecting amylase (hereinafter referred to as detection object), and amylase is detected on the surface of the detection object. The determination method according to any one of claims 1 to 10, wherein the sanitary treatment is a treatment using a sanitary treatment agent for cleaning, sterilization and/or virucidal. 12. The determination method according to claim 11, wherein the sanitary treatment agent contains one or more selected from (a) surfactants, (b) oxidative bleaching agents and (c) disinfectants. 13. The determination method according to claim 11 or 12, wherein the sanitary treatment includes contacting the sanitary treatment agent to the treated area and wiping the treated area contacted with the sanitary treatment agent with a flexible absorbent material. . A sanitary treatment method, comprising determining at least one of a location to be sanitized and conditions for sanitary treatment by the determination method according to any one of claims 1 to 13, and performing sanitary treatment based on the determination. 15. The sanitary treatment method according to claim 14, wherein the sanitary treatment is treatment using a sanitary treatment agent for at least one selected from washing, sterilization and virucidal. 16. The sanitary treatment method according to claim 15, wherein the sanitary treatment agent contains one or more selected from (a) a surfactant, (b) an oxidizing bleach and (c) a disinfectant. A sanitary treatment kit, comprising: means for detecting amylase in the environment; and an agent used for treating a sanitized site determined by the amylase detection result obtained by said means. An estimation method comprising detecting amylase in the environment and estimating locations where harmful viruses and/or harmful bacteria are likely to exist based on the detection results. An estimation method comprising detecting amylase in the environment, estimating locations where harmful viruses and/or harmful bacteria are likely to exist based on the detection results, and estimating infection routes. A method for displaying a contamination status at a candidate site for sanitary treatment, comprising:
(a) identifying one or more locations in the environment, comprising steps (i)-(iii) below;
(i) generating location data capable of identifying the location of the entity to identify the one or more locations within the defined area;
(ii) recording location data for said one or more locations within said environment; a step of separately recording;
(b) recording amylase detection results from one or more locations within the environment;
(c) recording the generated detection result for each segmented position and part of the physical entity recorded in (iii) of (a); and (d) recording the one or more records of (c) in the environment. a step of displaying together with the position data of
A method for displaying the contamination status at a candidate site for sanitary treatment, comprising: A method of displaying sanitary treatment locations, wherein the sanitary treatment location is determined based on the contamination status of the sanitary treatment candidate location displayed by the display method of claim 20, and displayed as the sanitary treatment location. 22. The sanitary processing location display method according to claim 21, wherein the possibility of contamination by viruses and/or bacteria is determined based on the contamination status, and the sanitary processing location is determined from the determination result.

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