TW202321447A - 包含crispr核酸酶之組成物及其用途 - Google Patents

包含crispr核酸酶之組成物及其用途 Download PDF

Info

Publication number
TW202321447A
TW202321447A TW111134168A TW111134168A TW202321447A TW 202321447 A TW202321447 A TW 202321447A TW 111134168 A TW111134168 A TW 111134168A TW 111134168 A TW111134168 A TW 111134168A TW 202321447 A TW202321447 A TW 202321447A
Authority
TW
Taiwan
Prior art keywords
variant polypeptide
nucleic acid
alteration
variant
alteration comprises
Prior art date
Application number
TW111134168A
Other languages
English (en)
Chinese (zh)
Inventor
少榮 仲
昆丁 諾曼 威瑟斯
羅伊 齊布雷
諾雅 麥可 杰奇莫
安東尼 詹姆士 葛瑞提
勞倫 Ｅ 雅方司
Original Assignee
美商阿伯生物技術公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 美商阿伯生物技術公司 filed Critical 美商阿伯生物技術公司
Publication of TW202321447A publication Critical patent/TW202321447A/zh

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/25—Site-specific endodeoxyribonucleases specific for altered bases (3.1.25)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
TW111134168A 2021-09-08 2022-09-08 包含crispr核酸酶之組成物及其用途 TW202321447A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202163241821P 2021-09-08 2021-09-08
US63/241,821 2021-09-08
US202263296741P 2022-01-05 2022-01-05
US63/296,741 2022-01-05

Publications (1)

Publication Number Publication Date
TW202321447A true TW202321447A (zh) 2023-06-01

Family

ID=85506919

Family Applications (1)

Application Number Title Priority Date Filing Date
TW111134168A TW202321447A (zh) 2021-09-08 2022-09-08 包含crispr核酸酶之組成物及其用途

Country Status (7)

Country Link
US (2) US12344870B2 (https=)
EP (1) EP4399310A4 (https=)
JP (1) JP2024531607A (https=)
AU (1) AU2022344256A1 (https=)
CA (1) CA3230609A1 (https=)
TW (1) TW202321447A (https=)
WO (1) WO2023039472A2 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3230609A1 (en) 2021-09-08 2023-03-16 Shaorong Chong Compositions comprising a crispr nuclease and uses thereof
WO2024173645A1 (en) 2023-02-15 2024-08-22 Arbor Biotechnologies, Inc. Gene editing method for inhibiting aberrant splicing in stathmin 2 (stmn2) transcript
WO2025171210A1 (en) 2024-02-09 2025-08-14 Arbor Biotechnologies, Inc. Compositions and methods for gene editing via homology-mediated end joining

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3241902B1 (en) 2012-05-25 2018-02-28 The Regents of The University of California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
US12431216B2 (en) 2016-08-17 2025-09-30 Broad Institute, Inc. Methods for identifying class 2 crispr-cas systems
PL3765615T3 (pl) * 2018-03-14 2023-11-13 Arbor Biotechnologies, Inc. Nowe enzymy i układy crispr ukierunkowane na dna
US12553045B2 (en) 2018-07-16 2026-02-17 Arbor Biotechnologies, Inc. Crispr DNA targeting enzymes and systems
US20220049273A1 (en) 2019-03-01 2022-02-17 Arbor Biotechnologies, Inc. Novel crispr dna targeting enzymes and systems
BR112022007125A2 (pt) * 2019-10-17 2022-07-05 Pairwise Plants Services Inc Variantes de nucleases cas12a e métodos de produção e uso das mesmas
CN115052986A (zh) * 2019-12-04 2022-09-13 阿伯生物技术公司 包含核酸酶的组合物及其用途
CA3230609A1 (en) 2021-09-08 2023-03-16 Shaorong Chong Compositions comprising a crispr nuclease and uses thereof

Also Published As

Publication number Publication date
WO2023039472A3 (en) 2023-08-24
US12344870B2 (en) 2025-07-01
US20250290053A1 (en) 2025-09-18
EP4399310A2 (en) 2024-07-17
JP2024531607A (ja) 2024-08-29
US20230235304A1 (en) 2023-07-27
EP4399310A4 (en) 2025-07-16
CA3230609A1 (en) 2023-03-16
AU2022344256A1 (en) 2024-02-29
WO2023039472A2 (en) 2023-03-16

Similar Documents

Publication Publication Date Title
CN115698278B (zh) 包含Cas12i2变体多肽的组合物及其用途
US20240174997A1 (en) Compositions comprising a variant polypeptide and uses thereof
US20240035010A1 (en) Compositions comprising a variant polypeptide and uses thereof
US20250290053A1 (en) Compositions comprising a crispr nuclease and uses thereof
US11946045B2 (en) Compositions comprising a variant polypeptide and uses thereof
US11866746B2 (en) Compositions comprising a variant Cas12i4 polypeptide and uses thereof
US20240352434A1 (en) Compositions comprising a variant cas12i3 polypeptide and uses thereof
EP4558625A1 (en) Compositions comprising a variant nuclease and uses thereof
US20240011004A1 (en) Compositions comprising a variant crispr nuclease polypeptide and uses thereof
CN118019846A (zh) 包含crispr核酸酶的组合物及其用途
CN117043326A (zh) 包含变体多肽的组合物及其用途
CN117136233A (zh) 包含变体Cas12i4多肽的组合物及其用途
CN117295816A (zh) 包含变体多肽的组合物及其用途