TW202220681A - Skin flora improving agent and skin topical agent for improving skin flora in which skin bacterial flora improving agent includes fermented milk whey obtained by fermenting milk with Lactobacillus helveticus, and a skin topical agent - Google Patents

Abstract

A skin flora improving agent includes fermented milk whey obtained by fermenting milk with Lactobacillus helveticus; and a skin topical agent for improving skin bacterial flora which comprises the skin bacterial flora improving agent. The present invention provides a skin bacterial flora improving agent which is highly safe and capable improving the skin bacterial flora, and further provides a skin topical agent for improving skin bacterial flora.

Description

Skin bacterial flora improving agent and skin external preparation for skin bacterial flora improving

The present invention relates to an agent for improving skin bacterial flora and an external preparation for skin for improving skin bacterial flora.

There are hundreds of species and hundreds of trillions of bacteria inhabiting the human body, covering the mouth, stomach, intestines, skin and other parts of the body, and forming inherent bacterial clusters with different bacterial species and compositions. In recent years, the most advanced intestinal flora (also known as "gut flora") is known to have various physiological roles. For example, beneficial effects include inhibition of colonization of pathogenic bacteria, activation of the immune system, and production of vitamins, and harmful effects include production of spoilage products or carcinogens, participation in various intestinal diseases, and the like. Therefore, it can be said that the intestinal flora is closely related to health.

In addition, the skin flora (also known as "skin flora", "skin flora", and "skin flora") has been attracting attention in recent years, and it is said that if the skin flora is well balanced and stable, it is easier to maintain skin health. The main bacterial genera of the skin bacterial flora include: Corynebactrium, Propionibacterium, Stapylococcus, β-Proteobacteria, γ-Proteobacteria (γ-Proteobacteria) class, it is reported that the bacterial flora of the skin varies according to the state of the skin (for example, see Non-Patent Document 1). It is known that Cedecea lapagei, Enterobacter asburiae, Enterobacter asburiae, Cedecea lapagei, Enterobacter The strains of Raoultella ornithinolytica, as well as the strains of Pseudomonas aeruginosa, Streptococcus pyogenes, and Enterococcus, have carcinogenic potential of harmful bacteria (for example, refer to Non-Patent Documents 1 to 5).

In addition, it has been reported that certain diseases are associated with the bacterial flora of the skin, while many reports of Propionibacterium acnes aggravating acne, Staphlococcus aureus and atopic dermatitis related (for example, refer to Non-Patent Documents 1 and 5). On the other hand, it has been reported that Staphylococcus epidermidis, which belongs to the same genus as Staphlococcus aureus, produces antimicrobial peptides against Staphylococcus aureus, enhances moisturizing power by strengthening the skin barrier, improves the The role of skin immunity is to act as beneficial bacteria in the skin bacterial flora (for example, refer to Non-Patent Documents 1 and 5).

As a method for evaluating skin condition, a method using the ratio of Xanthomonadaceae bacteria in skin bacterial flora as an index has been proposed (for example, refer to Patent Document 1).

However, there is still no material that can sufficiently satisfy the high safety requirements and can keep the skin bacterial flora in a good state, and there is an urgent need for rapid development.

In addition, it is reported that fermented whey obtained by fermenting milk with Lactobacillus helveticus can be used as a moisturizing agent (for example, refer to Patent Document 2), an epidermal cell differentiation and keratinization promoter (for example, See Patent Document 3), and active ingredients such as UV-sensitivity inhibitors in the skin (for example, see Patent Document 4).

[Prior Art Literature] [Patent Literature] [Patent Document 1] Japanese Patent Application Laid-Open No. 2017-209055 [Patent Document 2] Japanese Patent Laid-Open No. 2005-206578 [Patent Document 3] International Patent Publication No. 2006/137513 [Patent Document 4] International Patent Publication No. 2006/095764

[Non-patent literature] [Non-Patent Literature 1] Uwe Wollina. Microbiome in atopic Dermatitis, Clinical Cometic and Investigational Dermatology, (2017), 10:51-56. [Non-Patent Document 2] Chavez Herrera et al., Death related to Cedecea lapagei in a soft tissue bullae infection: a case report, Journal of Medical Case Reports, (2018) 12:328. [Non-Patent Document 3] Jalal Mardaneh et al., Isolation and Identification Enterobacter asburiae from Consumed Powdered Infant Formula Milk (PIF) in the Neonatal Intensive Care Unit (NICU), Acta Medica Iranica, Vol.54, No.1(2016) , 39-43. [Non-Patent Literature 4] Piseth Seng et al., Emerging role of Raoultella ornithinolytica in human infections: a series of cases and review of the literature, International Journal of Infectious Diseases, 45(2016), 65-71. [Non-Patent Document 5] Elizabeth A. Grice et al., The skin microbiome, Nat Rev Microbiol, 9(2011), 244-253.

[Problems to be Solved by Invention] The problem to be solved by the present invention is to solve the above-mentioned conventional problems and achieve the following objects. That is, an object of the present invention is to provide a skin bacterial flora improving agent and a skin external preparation for skin bacterial flora improving, which are highly safe and can keep the skin bacterial flora in a good state.

[means to solve the problem] In order to solve the above-mentioned problems, the present inventors have repeatedly studied and found that fermented whey obtained by fermenting milk with Lactobacillus helveticus has an effect of keeping the skin bacterial flora in a good state.

The present invention is made by the present inventors based on the above-mentioned findings, and as means for solving the above-mentioned problems, it is as follows. which is: <1> A skin flora improving agent characterized by containing fermented whey obtained by fermenting milk with Lactobacillus helveticus. <2> The skin bacterial flora improving agent according to <1>, wherein the improving skin bacterial flora is selected from the group consisting of maintaining the ratio of beneficial bacteria in the skin bacterial flora, increasing the ratio of beneficial bacteria, and the ratio of harmful bacteria at least one of the reductions. <3> The skin flora improving agent according to <2>, wherein the harmful bacteria are bacteria that may cause inflammation. <4> The skin flora improving agent according to any one of <1> to <3>, wherein the Lactobacillus helveticus is a Lactobacillus helveticus CM4 strain (accession number: FERM BP-6060). <5> A skin external preparation for improving skin bacterial flora, comprising the skin bacterial flora improving agent according to any one of <1> to <4>.

[Inventive effect] According to the present invention, it is possible to provide a skin bacterial flora improving agent and a skin bacterial flora improving skin external preparation which can solve the above-mentioned problems and achieve the above object, are highly safe, and can keep the skin bacterial flora in a good state.

(Skin bacterial flora improver) The skin flora improving agent of the present invention contains at least fermented whey obtained by fermenting milk with Lactobacillus helveticus (hereinafter referred to as "LH fermented whey"), and further contains other components as needed.

The skin bacterial flora improving agent has the effect of improving the state of the skin bacterial flora. In this specification, improving the state of the skin bacterial flora refers to keeping the skin bacterial flora in a good state (or "healthy state"), which also includes maintaining the good state. Preferably, the skin bacterial flora improving agent has at least one effect selected from the group consisting of maintaining the ratio of beneficial bacteria in the bacterial flora of the skin, increasing the ratio of beneficial bacteria, and reducing the ratio of harmful bacteria.

The beneficial bacteria are not particularly limited as long as they can keep the skin in a good state, and can be appropriately selected according to the purpose, and examples thereof include Staphylococcus epidermidis and the like.

The harmful bacteria are not particularly limited as long as they have an adverse effect on the skin condition, and can be appropriately selected according to the purpose. Branches of bacteria, Staphylococcus aureus, Propionibacterium acnes, Streptococcus pyogenes, etc.

The harmful bacteria having the possibility of causing inflammation is not particularly limited, and can be appropriately selected according to the purpose, and examples thereof include Staphylococcus aureus, Cedecea lapagei, At least one of the group consisting of Enterobacter asburiae and Raoultella ornithinolytica.

Although the details of the substances that LH-fermented whey possesses to improve the state of skin bacterial flora are not clear, the LH-fermented whey has such an excellent effect that its usefulness as a skin flora improving agent has not been known until now. It is unknown, but a new discovery by the inventors of the present invention.

＜LH Fermented Whey＞ - Lactobacillus helveticus - The strain of Lactobacillus helveticus is not particularly limited, and can be appropriately selected according to the purpose, but a strain with high extracellular protease activity is preferred. For example, it is preferable to follow the method of Yamamoto et al. (Yamamoto, N. etc J. Biochem. (1993) 114) based on the method of Twining et al. (Twining, S. Anal. Biochem. 143 3410 (1984)), 740) strains with a measured U/OD590 value of 400 or more. In addition, strains of Lactobacillus helveticus having the following mycological properties can be used.

[Bacteriological properties] 1. Morphological properties: 1) Cell shape: Bacillus 2) Mobility: none 3) With or without spores: none 4) Gram stain: positive 2. Physiological properties: 1) Catalase: negative 2) Indole production: negative 3) Nitrate reduction: negative 4) Performance on oxygen: facultative anaerobic bacteria 5) DL(-) lactic acid is produced by homolactic fermentation of glucose, and no gas is produced.

Among the Lactobacillus helveticus, the Lactobacillus helveticus CM4 strain is preferred. The described Lactobacillus helveticus CM4 strain was deposited in the Patent Microorganism Deposit Center of the National Institute of Industrial Technology and Research on August 15, 1997 with the deposit number FERM BP-6060 (1-1-1-1-1-1-1-1-1-1- 1). This Lactobacillus helveticus CM4 strain is registered under the above-mentioned deposit number in - Budapest Treaty of International Recognition of Microorganism Deposits on Patent Procedures, and this strain is patented.

The method for producing the LH fermented whey is not particularly limited, and can be appropriately selected according to the purpose. For example, the Lactobacillus helveticus is directly added to milk, or the fermented milk starter containing the Lactobacillus helveticus is added. It is obtained by adding it to milk, selecting fermentation conditions such as fermentation temperature appropriately, and fermenting it.

The LH fermented whey system as an active ingredient can be obtained by separating whey from the obtained fermented milk by conventional separation operations such as telecentric separation and filtration. It can be used in a milk state or by appropriately fractionating, concentrating, and purifying the separated whey, and in addition, the whey or its concentrate can also be used as a powder by freeze-drying, spray-drying, etc. and use.

Among the Lactobacillus helveticus, preferably, starter bacteria with sufficient high activity that have been pre-cultured in advance are used. The initial bacterial count is preferably about 10 ⁵ to 10 ⁹ cells/mL.

On the premise that the effect of the present invention is not impaired, other bacteria such as lactic acid bacteria and yeast other than Lactobacillus helveticus may also be included in the fermentation.

The raw material milk is not particularly limited and can be appropriately selected according to the purpose. Examples include animal milk such as cow's milk, horse's milk, goat's milk, and goat's milk; vegetable milk such as soy milk; skimmed milk and reduced milk such as processed milk , milk powder, condensed milk, etc., among which cow's milk, soy milk, and processed milk thereof are preferred, and cow's milk or its processed milk is more preferred. The solid content concentration of milk is not particularly limited and can be appropriately selected according to the purpose, but is preferably, for example, when skim milk is used. Preferably it is 6-15 mass %.

The fermentation is usually carried out by standing or stirring culture, for example, by the following method: the fermentation temperature is 25-45°C, preferably 30-45°C, and the fermentation time is 3-72 hours, preferably 12- 36 hours, and stop fermentation when the acidity of lactic acid reaches 1.5 or more.

The content in the skin flora improving agent of the LH-fermented whey is not particularly limited, and can be appropriately adjusted according to the physiological activity of the LH-fermented whey and the like. The skin flora improving agent may consist only of the LH fermented whey.

＜Other ingredients＞ The other components are not particularly limited unless they impair the efficacy of the present invention, and can be appropriately selected according to the purpose. The content of the other components in the skin bacterial flora improving agent is not particularly limited, and can be appropriately selected according to the purpose.

＜Use＞ The use of the skin flora improving agent of the present invention is not particularly limited, and can be appropriately selected according to the purpose, but for example, it can be favorably used as an active ingredient of the skin external preparation for skin flora improving described later.

The skin bacterial flora improving agent can be used every day, and through the action of LH fermented whey as an active ingredient, the skin bacterial flora improving effect can be exerted extremely effectively.

The daily usage amount of the skin bacterial flora improving agent varies depending on the object of use, the type of use, the use interval and other factors, and there are no special restrictions, but for example, as an adult, apply it to the skin of the face every day. The amount to be applied is preferably 0.01 to 5 g, more preferably 0.1 to 0.5 g, of the skin bacterial flora improving agent containing 0.01 to 20 mass % of the LH fermented whey. This daily dose can be used once or divided into multiple doses.

The use timing of the skin bacterial flora improving agent is not particularly limited, and can be appropriately selected according to the purpose. or intermittently.

The skin bacterial flora improving agent of the present invention can be well applied to humans, and can also be applied to animals other than humans under the premise of its action and efficacy.

In addition, the skin bacterial flora improving agent of the present invention can be used as a reagent for studying skin bacterial flora improving effect or skin bacterial flora.

(external preparation for skin bacterial flora improvement) The skin external preparation for improving skin flora of the present invention contains at least the skin flora improving agent of the present invention, and further contains other components as needed.

＜Skin bacterial flora improver＞ The skin bacterial flora improving agent is the above-mentioned skin bacterial flora improving agent of the present invention.

The content of the skin bacterial flora improving agent in the external preparation for skin bacterial flora improving is not particularly limited, and can be appropriately adjusted according to the physiological activity of LH fermented whey contained in the skin bacteria improving agent, etc. For example, the amount of the LH fermented whey is preferably 0.01 to 20% by mass, more preferably 0.05 to 10% by mass. In addition, the quantity of the said LH fermented whey means the mass when it becomes solid by drying. The skin external preparation system for improving skin bacterial flora may consist only of the skin bacterial flora improving agent.

＜Other ingredients＞ The other components in the external preparation for improving skin bacterial flora are not particularly limited as long as the efficacy of the present invention is not impaired, and can be appropriately selected according to the usage pattern of the external preparation for improving skin bacterial flora. , for example: oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, ultraviolet absorbers, thickeners agents, pigments, antioxidants, etc. In addition, substances having the effect of keeping the known skin bacterial flora in good condition may also be contained. These may be used individually by 1 type, and may use 2 or more types together. The content of the other components in the external preparation for skin bacterial flora improvement is not particularly limited, and can be appropriately selected according to the purpose.

The external preparation for skin is not particularly limited, and can be appropriately selected according to the purpose, and examples thereof include cosmetics, quasi-drugs, and pharmaceuticals. Among these, as shown in the test examples described later, when the external preparation for skin is applied, the moisture content of the skin and the texture of the skin are improved, so that it can be favorably used as a cosmetic.

The dosage form of the external preparation for skin flora improvement is not particularly limited, and can be appropriately selected according to the purpose. , poultice, essence, mask, cleanser, shower gel, foundation, etc.

The method for producing the external preparation for skin flora improvement is not particularly limited, and can be appropriately selected according to the dosage form. For example, various components can be mixed or emulsified using a conventional method to produce.

The skin external preparation for improving skin bacterial flora can be used every day, and through the action of LH fermented whey as an active ingredient, the skin bacterial flora can be improved very effectively.

The daily usage amount of the skin external preparation for improving skin bacterial flora varies depending on factors such as the object of use, the type of usage, and the usage interval, and is not particularly limited. The daily amount applied to the skin on the face is to apply 0.01 to 5 g of the external preparation for skin bacterial flora improvement mixed with 0.01 to 20 mass% of the LH fermented whey, and evenly apply 0.01 to 5 g, and apply evenly. 0.1～0.5g is more preferable. This daily dose can be used once or divided into multiple doses.

The timing of use of the external preparation for improving skin bacterial flora is not particularly limited, and can be appropriately selected according to the purpose. Use continuously or intermittently after exposure to such environments.

The external preparation for improving skin bacterial flora can be well applied to humans, and can also be applied to animals other than humans on the premise of exerting its effects.

[Example] Test examples and formulation examples of the present invention will be described below, but the present invention is not limited to these test agents and treatment examples.

(Test Example 1) As a lotion, a lotion (Lactina Moist Lotion, manufactured by Asahi Calpis Health Co., Ltd.) containing 5% by mass of fermented whey obtained from Lactobacillus helveticus CM4 strain (accession number: FERM) was prepared. BP-6060) is obtained by fermenting milk.

<Test method> 15 Japanese women aged 45 to 59 with an average age of 54.0±3.3 were asked to apply the above lotion for one week after washing their faces in the morning and evening.

- Analysis of skin bacterial flora - In the morning before the start of the application test and in the morning after application for a week, use a cotton swab soaked in physiological saline to wipe the forehead and cheeks 5 to 6 times before washing the face to collect samples. DNA was extracted from the collected samples, and the V2 to V4 and V6 to V9 regions of the 16S ribosomal protein amplified using the ION 16S metagenomics kit (manufactured by Thermo Fisher Scientific) were analyzed. Sequences were analyzed using a next-generation sequencer (Ion Personal Genome Machine ^™ (PGM ^™ ) sequencer, manufactured by Thermo Fisher Scientific). A Basic Local Alignment Search Tool (BLAST) search was performed on the obtained sequences, and the highest identical strain among strains with a similarity of more than 95% was assigned to each strain. In addition, low-quality bases, short fragments below 120 bp, and sequences with a similarity less than 95% were excluded. The results are shown in Table 1.

[Table 1] Proportion (%) There is a corresponding t-test strain Before applying after application Cedecea lapagei 2.861 0.054 <0.001 Enterobacter asburiae 2.099 0.009 <0.001 Raoultella ornithinolytica 1.086 0.007 0.001 Staphylococcus aureus 0.079 0.060 0.703 Staphylococcus epidermidis 4.812 9.196 0.138

As shown in Table 1, when a lotion containing fermented whey obtained by fermenting milk with Lactobacillus helveticus was applied, it was shown that Sildena larvae, a harmful bacteria that may cause inflammation (Cedecea lapagei), Enterobacter asburiae, and Raoultella ornithinolytica decreased (15th in all members). In addition, although there was no statistically significant difference, it was confirmed that the ratio of Staphylococcus epidermidis, which is considered to be beneficial bacteria, is related to the moisturizing effect of the skin and has the effect of inhibiting the proliferation of harmful bacteria, increased in 15 students. Nine out of 15, the ratio of Staphylococcus aureus, which is considered a harmful bacteria, decreased by 8. Therefore, the fermented whey system obtained by fermenting milk with Lactobacillus helveticus has been shown to convert the skin bacterial flora into a healthy state and maintain this state.

─Skin moisture content, skin texture (texture volume ratio, texture number) ─ Before the start of the application test and one week after application, the skin moisture content and skin texture (texture volume ratio, texture number) of the right cheek were measured. The measurement of the skin moisture content was performed using Corneometer CM825 (manufactured by Ingray Co., Ltd.). The results are shown in Figure 1 . The measurement of skin texture was carried out by making a replica of the skin and using an analysis system (Replica Analysis System for Reflection, Asahi Biomedical Co., Ltd.). The measurement results of the texture volume ratio are shown in FIG. 2 , and the measurement results of the texture quantity are shown in FIG. 3 .

As shown in FIGS. 1 to 3 , after applying a lotion containing fermented whey obtained by fermenting milk with Lactobacillus helveticus, the skin moisture content, texture volume ratio, and texture number increased significantly, and it was confirmed that To be useful as an external preparation for the skin.

(Test Example 2: The harmful bacteria inhibitory effect of fermented whey was tested based on a culture experiment) Whether or not the harmful bacteria inhibitory action identified in Test Example 1 was a direct action of fermented whey obtained by fermenting milk with Lactobacillus helveticus was performed in the following manner by in vitro tests.

<Preparation of fermented milk whey> In a milk medium formed of skimmed milk powder with a solid content of 9% by mass, any one of the following lactic acid bacteria was inoculated (final inoculation amount: 1 to 2 × 10 ⁶ bacteria/mL), The fermented milk was obtained by stationary culture at 35°C for 24 hours. The obtained fermented milk was subjected to ultrafiltration (MW<5kDa) to obtain various fermented whey. [Lactic acid bacteria] The following lactic acid bacteria were used. In addition, the lactic acid bacteria strains other than the Lactobacillus helveticus CM4 strain used the respective standard strains.・Lactobacillus helveticus CM4 strain ・Lactobacillus helveticus ・Lactobacillus delbrueckii bulgaricus ・Streptococcus thermophilus ・Lactobacillus acidophilus ・Lactobacillus gasseri ・Cheese Lactobacillus casei

<Test method> (1) Inoculate a nutrient medium (Nutrient Broth, manufactured by BD, Japan) with Cedecea lapagei, Enterobacter asburiae, or Raoultella ornithinolytica ) for pre-incubation. (2) The various fermented whey prepared above were added to the nutrient culture liquid at 5% by mass, inoculated with 3% of any one of the three strains cultivated in the above (1), and cultured at 37°C for 16 to 24 Hour. The group to which fermented whey was not added was set as a control. (3) Measure the optical density (Optical Density, OD) (600 nm) after culture, and calculate the relative value when the OD value of the control group is 100. (4) Carry out three experiments, calculate the average value, and perform statistical analysis by Student's t-test. The results are shown in FIG. 4 .

As shown in FIG. 4 , even among lactic acid bacteria, only the fermented whey obtained by fermenting milk with Lactobacillus helveticus has the effect of suppressing harmful bacteria resident in skin bacteria.

(Prescription Example 1: Ointment) An ointment having the following composition was produced according to a conventional method. In addition, as fermented whey, in Test Example 2, fermented whey prepared with Lactobacillus helveticus CM4 strain was used. ・Fermented whey ・・・ 0.05% by mass ・Benzalkonium chloride             ・Urea・・・ 20.0% by mass ・White Vaseline   ・Light liquid paraffin・・・6.0% by mass ・Cetyl alcohol 3.0% by mass ・Stearyl alcohol 3.0% by mass ・Glyceryl monostearate ・・・ 5.0% by mass ・Fragrance            ・Preservatives ・・・ Appropriate amount ・Buffering agent ・Purified water・・・The rest Total 100.0% by mass

(Prescription Example 2: Cosmetic Liquid) Cosmetic liquids having the following compositions were produced according to conventional methods. In addition, as fermented whey, in Test Example 2, fermented whey prepared with Lactobacillus helveticus CM4 strain was used. ・Fermented whey ・・・ 5.0% by mass ・Lactic acid ・・・ 0.1% by mass ・Fruit acid              ・Glycerin   ・Kaolin Clay 1.0% by mass ・Calamine ・Camphor・・・ 0.2% by mass ・Ethanol・・・ 14.0% by mass ・Fragrance            ・Purified water・・・The rest Total 100.0% by mass

(Prescription Example 3: Cream) A cream having the following composition was produced according to a conventional method. In addition, as fermented whey, in Test Example 2, fermented whey prepared with Lactobacillus helveticus CM4 strain was used. ・Fermented whey ・・・ 8.0% by mass ・Natural vitamin E  ・Glycyrrhiza flavonoids 0.005% by mass ・Solid paraffin wax 5.0% by mass ・Beeswax 10.0% by mass ・Vaseline 15.0% by mass ・Liquid paraffin 41.0% by mass ・1,3-Butanediol ・・・4.0% by mass ・Glyceryl monostearate ・・・ 2.0% by mass ・Polyoxyethylene sorbitan monolaurate (20) ・・・ 2.0% by mass ・Various amino acids ・・・ 1.0% by mass ・Borax・・・ 0.2% by mass ・Preservatives ・・・ Appropriate amount ・Fragrance            ・Antioxidants          ・Purified water・・・The rest Total 100.0% by mass

(Formulation Example 4: Emulsion) An emulsion having the following composition was produced according to a conventional method. In addition, as fermented whey, in Test Example 2, fermented whey prepared with Lactobacillus helveticus CM4 strain was used. ・Fermented whey ・・・ 1.0% by mass ・Lactic acid ・・・ 2.0% by mass ・Stearyl alcohol   ・Hardened palm oil  ・Liquid paraffin 35.0% by mass ・Dipropylene glycol              ・Polyethylene glycol (400)         ・Sorbit sesquioleate ・・・ 1.6% by mass ・Polyoxyethylene (20) oleyl ether       ・Carboxyvinyl polymer ・・・ 1.5% by mass ・Potassium Hydroxide   ・Chelating agent ・Preservatives ・・・ Appropriate amount ・Fragrance            ・Purified water・・・The rest Total 100.0% by mass

(Prescription example 5: Cosmetic liquid) A cosmetic liquid having the following composition was produced according to a conventional method. In addition, as fermented whey, in Test Example 2, fermented whey prepared with Lactobacillus helveticus CM4 strain was used. ・Fermented whey ・・・ 3.0% by mass ・Fruit acid              ・Dipropylene glycol              ・Polyethylene glycol (400)     ・Ethanol    ・Carboxy vinyl polymer ・・・ 0.5% by mass ・Sodium alginate           ・Potassium Hydroxide             ・Polyoxyethylene monostearate (20) Sorbitan ・・・ 1.0% by mass ・Sorbitol monooleate・・・ 0.5% by mass ・Oleyl alcohol            ・Placenta Extract                                                            ・dl-α-tocopherol acetate ・・・ 0.2% by mass ・Fragrance            ・Preservatives ・・・ Appropriate amount ・Anti-fading agent・・・Appropriate amount ・Purified water・・・The rest Total 100.0% by mass

[deposit number] FERM BP-6060

none.

FIG. 1 is a graph showing the measurement results of skin moisture content in Test Example 1. FIG. FIG. 2 is a diagram showing the measurement results of the texture volume ratio in Test Example 1. FIG. FIG. 3 is a diagram showing the measurement results of the number of textures in Test Example 1. FIG. [ Fig. 4] Fig. 4 is a graph showing the results of measurement of the growth inhibitory effect of fermented whey using various lactic acid bacteria on harmful bacteria in Test Example 2. [Fig.

none.

Claims (5)

A skin bacterial flora improving agent characterized by containing fermented whey obtained by fermenting milk with Lactobacillus helveticus. The skin bacterial flora improving agent according to claim 1, wherein the improvement of the skin bacterial flora is selected from the group consisting of maintaining the ratio of beneficial bacteria, increasing the ratio of beneficial bacteria, and reducing the ratio of harmful bacteria in the bacterial flora of the skin at least one of them. The skin flora improving agent according to claim 2, wherein the harmful bacteria are bacteria that may cause inflammation. The skin bacterial flora improving agent according to any one of claims 1 to 3, wherein the Lactobacillus helveticus is a strain of Lactobacillus helveticus CM4 (accession number: FERM BP-6060). A skin external preparation for improving skin bacterial flora, characterized by containing the skin bacterial flora improving agent according to any one of claims 1 to 4.

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