TW202214671A - 人乳頭瘤病毒多價免疫原性組合物 - Google Patents
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Abstract
本發明關於一種嵌合的乳頭瘤病毒L1蛋白和編碼其的多核苷酸,HPV類病毒顆粒以及包含至少一種上述類病毒顆粒的多價HPV免疫原性組合物及其用途。所述嵌合的乳頭瘤病毒L1蛋白包含衍生於第一型別乳頭瘤病毒L1蛋白的N端片段,所述N端片段保持HPV相應型別L1蛋白的免疫原性;和衍生於第二型別乳頭瘤病毒L1蛋白的C端片段,所述第二型別乳頭狀瘤病毒L1蛋白具有相較於其他型別的L1蛋白表達量和可溶性較好的特性;其中所述嵌合的乳頭瘤病毒L1蛋白具有HPV相應型別L1蛋白的免疫原性。所述嵌合的乳頭瘤病毒L蛋白具有較高的表達量和可溶性,可用於疫苗的大規模生產。在一個實施方式中,至少一種所述HPV類病毒顆粒為嵌合的HPV類病毒顆粒,所述嵌合的HPV類病毒顆粒包含一種或多種嵌合HPV L1蛋白。
Description
本發明關於乳頭瘤病毒(HPV)L1蛋白和編碼該蛋白的多核苷酸,還關於HPV類病毒顆粒及含有HPV類病毒顆粒的預防人乳頭瘤病毒(HPV)相關疾病或感染的多價免疫原性組合物及其用途。
乳頭瘤病毒(papilloma virus,PV)屬於乳頭瘤病毒科(Papillomaviridae),能引起人、牛、狗、兔等的乳頭瘤。其成員人乳頭瘤病毒(Human Papillomavirus, HPV)為無包膜DNA病毒。該病毒的基因組為雙鏈閉環DNA,大小約7.2-8 kb,具有8個開放閱讀框,按照功能可分為三個區域:(1)早期區(E),約4.5 kb,編碼E1、E2、E4-E7共6個與病毒複製、轉錄及轉譯有關的非結構蛋白;(2)晚期區(L),約2.5kb,編碼主要衣殼蛋白L1和次要衣殼蛋白L2;(3)長調控區(LCR),其位於L區末端與E區起始端之間,長約800-900 bp,不編碼任何蛋白,但具有DNA複製和表達調控元件。
L1和L2蛋白在HPV感染週期的中晚期合成。L1蛋白是主要衣殼蛋白並且具有55-60 kDa的分子量。L2蛋白是次要衣殼蛋白。72個L1蛋白五聚體構成二十面體HPV病毒粒子的外殼(直徑為45-55 nm),其包裹閉環雙鏈DNA。L2蛋白質位於L1蛋白質內側(Structure of Small Virus-like Particles Assembled from the L1 Protein of Human Papillomavirus 16 Chen, X.S., R. L.Garcea, Mol.Cell. 5(3):557-567, 2000)。
L1蛋白的ORF是PV基因組中最保守的基因,可以用於鑒別新的PV型。如果選殖了完整的基因組,並且L1 ORF的DNA序列與最接近的已知PV型相差超過10%,則被認定為分離出新的PV型。差異在2%和10%同源性被定義為不同的亞型,差異小於2%被定義為同一亞型的不同變種(E.-M. de Villiers et al. / Virology 324 (2004) 17–27)。
在HPV感染的後期,細胞質中新合成的L1蛋白被輸送到終端分化的角蛋白細胞核中,與L2蛋白一起,包裝複製的HPV基因組DNA形成傳染性病毒(Nelson, L.M, et al. 2002. Nuclear import strategies of high risk HPV16 L1 major capsid protein. J. Biol. Chem. 277: 23958-23964)。這表明L1蛋白的核導入在HPV感染和生產中起著非常重要的作用。病毒進入細胞核的能力由HPV L1蛋白C端的核定位信號(NLS)決定,核定位信號的一個特徵是富含鹼性胺基酸(Garcia-Bustos, J., et al. 1991. Nuclear protein localization. Biochimica et Biophysica Acta 1071: 83-101)。
15種高風險(HR) HPV型可導致子宮頸、肛門、陰莖、陰道、外陰和口咽癌。其中,HPV-16和HPV-18型是迄今最為常見的癌症起因,約佔子宮頸癌的70%,其餘為其他HR-HPV型(31、33、35、39、45、51、52、56、58、59、68、73和82)引起。HPV-16約占HPV陽性口咽癌(OPCs)的95%。持續低風險基因型HPV-6和HPV-11導致大多數肛門生殖器疣和呼吸道乳頭狀瘤,但很少與癌症相關(Human Papillomavirus in Cervical Cancer and Oropharyngeal Cancer: One Cause, Two Diseases Tara A. Bermanand John T. Schiller, PhD2 Cancer 2017;123:2219-29)。
使用痘瘡病毒、桿狀病毒或酵母系統重組表達L1蛋白,L1蛋白可自我裝配形成類病毒顆粒(VLP),大約含有72個L1蛋白,與病毒體外殼相似。VLP沒有適應症。VLP可以在接種動物中誘導中和抗體,保護實驗動物免受感染性病毒的隨後攻擊。因此,VLP似乎是乳頭瘤病毒疫苗的優秀候選者(Structure of Small Virus-like Particles Assembled from the L1 Protein of Human Papillomavirus 16 Chen, X.S., R. L.Garcea, Mol.Cell. 5(3):557-567, 2000)。
葛蘭素公司的CERVARIX
®是雙價重組HPV疫苗。其中含有由重組桿狀病毒表達載體系統在夜蛾(Trichoplusia ni)昆蟲細胞中表達獲得的HPV 16型重組L1蛋白和HPV 18型重組L1蛋白。L1蛋白自組裝成類病毒顆粒,用於預防9-25歲的婦女由16和18型HPV引起的子宮頸癌,2級或3級子宮頸上皮內瘤樣變和原位腺癌,和1級子宮頸上皮內瘤樣病變(致癌)
(https://www.fda.gov/downloads/BiologicsBloodVaccines/Vaccines/ApprovedProducts/UCM186981.pdf)。
GARDASIL
®是默克公司生產的人乳頭狀瘤病毒四價(6、11、16和18型)重組疫苗,用於9-26歲的女孩和婦女用於預防子宮頸癌生殖器疣(尖銳濕疣)和由HPV 6、11、16、18型引起癌前或增生異常病變;以及9-26歲的男孩和男人用於預防肛門癌、生殖器疣(尖銳濕疣)和由HPV 6、11、16、18型引起的癌前期或發育異常病變
(https://www.fda.gov/vaccines-blood-biologics/vaccines/gardasil)。
GARDASIL
®9是默克公司生產的人乳頭狀瘤病毒九價重組疫苗,包含HPV 6、11、16、18、31、33、45、52和58型L1蛋白的類病毒顆粒,該L1蛋白由釀酒酵母發酵生產,自組裝為VLP。用於9-45歲的女孩和婦女用於預防HPV16、18、31、33、45、52和58型引起的子宮頸癌、外陰癌、陰道癌和肛門癌,HPV6和11引起的生殖器疣 (尖銳濕疣)和由HPV 6、11、16、18、31、33、45、52和58型引起癌前或或增生異常病變;以及9-45歲的男孩和男人用於預防16、18、31、33、45、52和58型引起的肛門癌,HPV 6和11引起的生殖器疣(尖銳濕疣)和由HPV 6、11、16、18、31、33、45、52和58型引起的癌前期或發育異常病變(https://www.fda.gov/vaccines-blood-biologics/vaccines/gardasil-9)。
GARDASIL
®9的說明書中聲稱HPV16和18型是約70%的子宮頸癌的發病緣由,其餘的20%病例歸責於31、33、45、52和58型,由是GARDASIL
®9可以預防90%的子宮頸癌的發生(https://www.fda.gov/BiologicsBloodVaccines/Vaccines/ApprovedProducts/ucm426445.htm)。
HPV疫苗研製的關鍵因素是類病毒顆粒可進行大量生產。目前較為普遍的生產類病毒顆粒的系統主要分為真核表達系統和原核表達系統。
常用的真核表達系統有痘病毒表達系統、昆蟲桿狀病毒表達系統、酵母表達系統。在真核表達系統中表達的HPV L1蛋白的天然構型破壞較少,可自發裝配形成類病毒顆粒,但產量較低。原核表達系統主要大腸桿菌表達系統,產量高但大多以包涵體形式存在,不利於純化,生產程序複雜。
因此,在本領域仍然存在獲得高產量的HPV類病毒顆粒,從而獲得HPV多價疫苗以廣效地預防HPV相關疾病或感染,包括目前商業化疫苗尚未涵蓋的HPV型別引起的HPV相關疾病或感染。
在一個方面,本發明提供一種嵌合的乳頭瘤病毒L1蛋白,自其N末端至C末端方向包含a. 衍生於第一型別乳頭瘤病毒L1蛋白的N端片段,所述N端片段保持HPV相應型別L1蛋白的免疫原性;和b. 衍生於第二型別乳頭瘤病毒L1蛋白的C端片段,所述第二型別乳頭狀瘤病毒L1蛋白具有相較於其他型別的L1蛋白表達量和可溶性較好的特性;其中所述嵌合的乳頭瘤病毒L1蛋白具有第一型別乳頭瘤病毒L1蛋白的免疫原性。
在另一個方面,本發明提供一種乳頭瘤類病毒顆粒,其包含嵌合的乳頭瘤病毒L1蛋白。
在另一個方面,本發明提供一種分離的多核苷酸,其編碼嵌合的乳頭瘤病毒L1蛋白。
在另一個方面,本發明提供一種載體,其包含編碼嵌合的乳頭瘤病毒L1蛋白的多核苷酸。
在另一個方面,本發明提供一種桿狀病毒,其包含編碼嵌合的乳頭瘤病毒L1蛋白的多核苷酸。
在另一個方面,本發明提供一種宿主細胞,其包含如前所述的多核苷酸、載體、或桿狀病毒。
在一個方面,本發明提供一種預防HPV相關疾病或感染的HPV免疫原性組合物,其包含以上方面的嵌合的乳頭瘤病毒L1蛋白、HPV類病毒顆粒、多核苷酸、載體、桿狀病毒、細胞;
在一個具體的實施方案中,其包含:由HPV 6型、11型、16型、18型、31型、33型、45型、52型和58型的嵌合的L1蛋白組裝而成的HPV類病毒顆粒;和HPV33型和59型的L1蛋白組裝而成的HPV類病毒顆粒。
在另一個方面,本發明提供一種預防HPV相關疾病或感染的方法,其包括:向受試者施用多價HPV免疫原性組合物。
在另一個方面,本發明提供多價HPV免疫原性組合物在用於製備用於預防HPV相關疾病或感染的疫苗或藥物中的用途。
在一個方面,本發明提供一種嵌合的乳頭瘤病毒L1蛋白,自其N末端自C末端方向包含:a. 衍生於第一型別乳頭瘤病毒L1蛋白的N端片段,所述N端片段保持HPV相應型別L1蛋白的免疫原性;和b. 衍生於第二型別乳頭瘤病毒L1蛋白的C端片段,所述第二型別乳頭狀瘤病毒L1蛋白具有相較於其他型別的L1蛋白表達量和可溶性較好的特性;其中所述嵌合的乳頭瘤病毒L1蛋白具有第一型別乳頭瘤病毒L1蛋白的免疫原性。
在一個實施方式中,所述N端片段為將所述第一型別乳頭瘤病毒L1蛋白的天然序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段,以及與其具有至少98%的同一性的片段;所述C端片段為將第二型別乳頭狀瘤病毒L1蛋白的天然序列的N末端截短於其α5區內的任一胺基酸位點而得到的片段,以及該片段進一步突變、缺失和/或添加而產生的功能性變體。
在另一個實施方式中,所述N端片段與將所述第一型別乳頭瘤病毒L1蛋白天然序列的C端截短於其α5區內的任一胺基酸位點而得到的片段具有至少98.5%、99%、99.5%或100%的同一性。
在一個實施方式中,所述C端片段含有一個或多個核定位序列。
在一個實施方式中,所述乳頭瘤病毒L1蛋白是HPV L1蛋白。
在一個實施方式中,所述第二型別乳頭瘤病毒L1蛋白選自HPV 1型、2型、3型、4型、6型、7型、10型、11型、13型、16型、18型、22型、26型、28型、31型、32型、33型、35型、39型、42型、44型、45型、51型、52型、53型、56型、58型、59型、60型、63型、66型、68型、73型或82型L1蛋白;
較佳地,所述第二型別乳頭瘤病毒L1蛋白選自HPV 16型、28型、33型、59型、或68型L1蛋白;
更較佳地,所述第二型別乳頭瘤病毒L1蛋白選自HPV 33型或HPV 59型L1蛋白。
在一個實施方式中,所述第二型別乳頭瘤病毒L1蛋白為HPV 33型L1蛋白,,所述C端片段為SEQ ID No: 2;或其長度為m1個胺基酸的片段,較佳涵蓋SEQ ID No: 2的第1-m1位胺基酸的片段;其中m1為8-26的整數;或所述C端片段為SEQ ID No: 135;或其長度為m2個胺基酸的片段,較佳涵蓋SEQ ID No: 135的第1-m2位胺基酸的片段;其中m2為13-31的整數。
在一個實施方式中,HPV 33型L1蛋白的C端片段具有一個核定位序列。在另一個實施方式中,HPV 33型L1蛋白的C端片段具有兩個核定位序列。在一些實施方式中,嵌合的乳頭瘤病毒L1蛋白包含一個或多個HPV 33型L1蛋白的C端片段。所述多個HPV 33型L1蛋白的C端片段可以相同也可以不同。在一個實施方式中,SEQ ID No: 2的胺基酸編號7-8的胺基酸序列(KR)和胺基酸序列編號20-23的胺基酸序列(KRKK)為HPV 33型L1蛋白的C端片段的核定位序列。
在另一個實施方式中,所述第二型別乳頭瘤病毒L1蛋白為HPV 59型L1蛋白,所述C端片段為SEQ ID No: 13;或其長度為n個胺基酸的片段,優選涵蓋SEQ ID No: 13的第1-n 位胺基酸的片段;其中n為16-38的整數。
在一個實施方式中,HPV 59型L1蛋白的C端片段具有一個核定位序列。在另一個實施方式中,HPV 59型L1蛋白的C端片段具有兩個核定位序列。在一些實施方式中,嵌合的乳頭瘤病毒L1蛋白包含一個或多個HPV 59型L1蛋白的C端片段。所述多個HPV 59型L1蛋白的C端片段可以相同也可以不同。
在一個實施方式中,所述第一型別乳頭瘤病毒L1蛋白選自HPV 6型、11型、16型、18型、31型、35型、39型、45型、51型、52型、56型或58型L1蛋白。
在一個實施方式中,所述HPV 6型L1蛋白的N端片段與將SEQ ID No: 1所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性;
所述HPV 11型L1蛋白的N端片段與將SEQ ID No: 14所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性;
所述HPV 16型L1蛋白的N端片段與將SEQ ID No: 27所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性;
所述HPV 18型L1蛋白的N端片段與將SEQ ID No: 40所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性;
所述HPV 31型L1蛋白的N端片段與將SEQ ID No: 53所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性;
所述HPV 35型L1蛋白的N端片段與將SEQ ID No: 69所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性;
所述HPV 39型L1蛋白的N端片段與將SEQ ID No: 82所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性;
所述HPV 45型L1蛋白的N端片段與將SEQ ID No: 95所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性;
所述HPV 51型L1蛋白的N端片段與將SEQ ID No: 108所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性;
所述HPV 52型L1蛋白的N端片段與將SEQ ID No: 121所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性;
所述HPV 56型L1蛋白的N端片段與將SEQ ID No: 134所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性;和
所述HPV 58型L1蛋白的N端片段與將SEQ ID No: 147所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性。
在一個實施方式中,所述N端片段的C末端與所述C端片段的N末端直接連接或透過連接子連接。
連接子不影響所述N端片段的免疫原性,且不影響蛋白的表達量或可溶性。在一個實施方式中,所述N端片段和所述C端片段透過由1、2、3、4、5、6、7、8、9或10個胺基酸組成的連接子連接。在一個實施方式中,連接子是人工序列。在另一個實施方式中,連接子是HPV L1蛋白中天然存在的序列。在另一個實施方式中,連接子可以是HPV 33型L1蛋白的部分序列。在另一個實施方式中,連接子可以是HPV 59型L1蛋白的部分序列。
在一個實施方式中,所述N端片段的C末端與所述C端片段的N末端連接時,在連接點的正負4個胺基酸位點的範圍內存在以下連續胺基酸序列:RKFL;較佳地,在連接點的正負6個胺基酸位點的範圍內存在以下連續胺基酸序列:LGRKFL。
在一些實施方式中,所述嵌合的HPV 6型、11型、16型、18型、31型、35型、39型、45型、51型、52型、56型和58型嵌合HPV L1蛋白分別與SEQ ID No: 3、SEQ ID No: 16、SEQ ID No: 29、SEQ ID No: 42、SEQ ID No: 55、SEQ ID No: 71、SEQ ID No: 84、SEQ ID No: 97、SEQ ID No: 110、SEQ ID No: 123、SEQ ID No: 136和SEQ ID No: 149具有98%、98.5%、99%、99.5%或100%的同一性。以及HPV 33型L1蛋白和HPV 59型L1蛋白分別與SEQ ID No: 66和SEQ ID No: 160具有98%、98.5%、99%、99.5%或100%的同一性。
在一個方面,本發明提供一種乳頭瘤類病毒顆粒,其包含如前所述的嵌合的乳頭瘤病毒L1蛋白。在一個實施方式中,乳頭瘤類病毒顆粒是HPV類病毒顆粒,在一個實施方式中,HPV類病毒顆粒為由72個所述嵌合的HPV L1蛋白的五聚體構成的二十面體。在一個實施方式中,HPV類病毒顆粒具有正確形成的雙硫鍵,因而具有良好的天然構型。在一個實施方式中,HPV類病毒顆粒在體內表達系統中自行裝配。
在一個方面,本發明提供一種分離的多核苷酸,其編碼如前所述的嵌合的乳頭瘤病毒L1蛋白。在一個實施方式中,多核苷酸為針對不同表達系統進行密碼子優化後的多核苷酸。在一個實施方式中,多核苷酸為針對昆蟲桿狀病毒表達系統進行密碼子優化後的多核苷酸。
在一個方面,本發明提供一種載體,其包含如前所述的多核苷酸。在一個實施方式中,載體為桿狀病毒載體。在一個實施方式中,載體可以是用於桿狀病毒表達系統的轉移載體。在另一個實施方式中,載體可以是用於桿狀病毒表達系統的表達載體。在另一個實施方式中,載體可以是用於桿狀病毒表達系統的重組後的載體。
在一個方面,本發明提供一種桿狀病毒,其包含如前所述的多核苷酸。
在一個方面,本發明提供一種宿主細胞,其包含如前所述的多核苷酸、載體、或桿狀病毒。在一個實施方式中,宿主細胞為昆蟲細胞,較佳地,所述昆蟲細胞選自Sf9細胞、Sf21細胞、Hi5細胞和S2細胞。
在一個方面,本發明提供一種預防乳頭瘤病毒相關的疾病或感染的多價免疫原性組合物。其包含以上方面的HPV類病毒顆粒、多核苷酸、載體、桿狀病毒、和細胞。
在一個實施方式中,其包含:由嵌合的L1蛋白,如HPV 6型、11型、16型、18型、31型、35型、39型、45型、51型、52型、56型和58型的L1蛋白組裝而成的HPV類病毒顆粒;和一種或多種由其他致病的HPV型別,如HPV33型和59型的L1蛋白組裝而成的HPV類病毒顆粒。
在一個實施方式中,所述HPV各型別的L1蛋白可以為天然存在的L1蛋白,或非天然存在的L1蛋白,或嵌合HPV L1蛋白。在一個實施方式中,所述HPV類病毒顆粒可以由單一型別的HPV L1蛋白裝配形成單價的HPV類病毒顆粒,也可以由多個型別的HPV L1蛋白共同裝配形成多價的HPV類病毒顆粒。
在一個實施方式中,所述嵌合的HPV類病毒顆粒可以由單一型別的嵌合HPV L1蛋白裝配形成單價的HPV類病毒顆粒,也可以由多個型別的嵌合HPV L1蛋白共同裝配形成多價的HPV類病毒顆粒。
在一些實施方式中,所述嵌合的HPV 6型、11型、16型、18型、31型、35型、39型、45型、51型、52型、56型和58型嵌合HPV L1蛋白分別與SEQ ID No: 3、SEQ ID No: 16、SEQ ID No: 29、SEQ ID No: 42、SEQ ID No: 55、SEQ ID No: 71、SEQ ID No: 84、SEQ ID No: 97、SEQ ID No: 110、SEQ ID No: 123、SEQ ID No: 136和SEQ ID No: 149具有98%、98.5%、99%、99.5%或100%的同一性;以及HPV 33型L1蛋白和HPV 59型L1蛋白分別與SEQ ID No: 66和SEQ ID No: 160具有98%、98.5%、99%、99.5%或100%的同一性。
在一個實施方式中,至少一種所述HPV類病毒顆粒由單一型別的嵌合HPV L1蛋白組成,較佳地,由具有相同的胺基酸序列的所述單一型別的嵌合HPV L1蛋白組成。
在一個實施方式中,嵌合的HPV類病毒顆粒為由72個所述嵌合HPV L1蛋白的五聚體構成的二十面體。在一個實施方式中,嵌合的HPV類病毒顆粒具有正確形成的雙硫鍵,因而具有良好的天然構型。在一個實施方式中,嵌合的HPV類病毒顆粒在體內表達系統中自行裝配。
在一個實施方式中,所述多價HPV免疫原性組合物還包含生理學上可接受的載體以及任選地,還包含佐劑。在一個實施方式中,佐劑包含鋁鹽、脂質A衍生物和ISCOM中的一種或多種。
在一個實施方式中,所述佐劑為磷酸鋁佐劑。
在一個方面,本發明提供一種預防HPV相關疾病或感染的方法,其包括:向受試者施用多價HPV免疫原性組合物。所述預防可被認為是治療,兩者可互換使用。在一個實施方式中,受試者是人。
在一個方面,本發明提供如前所述的多價HPV免疫原性組合物在用於製備用於預防HPV相關疾病或感染的疫苗或藥物中的用途。
真核表達系統表達的乳頭瘤病毒L1蛋白能自發裝配成類病毒顆粒,但具有表達量低不易規模化生產的缺點。
各型別HPV 的L1蛋白的序列可以從https://www.uniprot.org 方便地獲得。每一型別的HPV L1可以來源於不同的病毒株,因而其胺基酸序列有多個版本,其中任何一個版本的天然序列都可以用於本發明,本發明的構思和設計過程中,所用某一給定型別的HPV L1蛋白序列有可能不同於實施例中使用的序列,但是這種差異不影響發明人的判斷和結論。
本發明所屬技術領域中具有通常知識者普遍認為L1蛋白的C端不含有主要中和抗原表位,因此試圖透過截短HPV L1蛋白的C端提高表達量,例如葛蘭素公司的美國專利US6361778B1中,HPV16 L1蛋白C端截短1-34個胺基酸,較佳26個胺基酸,聲明VLP的產量增加許多倍,最好至少增加10倍,特別是大約10到100倍。受此啟發,發明人嘗試將HPV 16型L1的C端截短31個胺基酸,命名為HPV16 L1(1-474)。但其蛋白表達量高但蛋白可溶性差,難以提取純化(見對比例)。
這種截短引起的蛋白可溶性差有可能是C端的核定位序列的缺失造成的,本發明並不受限於此推測。發明人在研究和生產過程中發現HPV 16型L1蛋白、HPV 28型L1蛋白、HPV 33型L1蛋白、HPV 59型L1蛋白和HPV 68型L1蛋白相較於其他型別的L1蛋白表達量和可溶性較好,受此啟發,發明人用表達量和可溶性較好型別的L1 蛋白C端替換不易提取或表達量低的HPV型別的C端。即發明人構建了這樣一種嵌合蛋白:自其N末端至C末端方向包含衍生於第一型別乳頭瘤病毒L1蛋白(例如HPV L1蛋白)的N端片段和衍生於第二型別乳頭瘤病毒L1蛋白(例如HPV L1蛋白)的C端片段,前者提供第一型別乳頭瘤病毒(例如HPV)的免疫原性,後者提供表達量和可溶性較好的特性。兩者可以直接連接也可以透過連接子連接。
為保持第一型別HPV L1蛋白的免疫原性,以及保證其能夠形成VLP,發明人確定了合適的HPV L1蛋白的N端片段的長度。以下報導關於常見HPV亞型的表位研究:
Sunanda Baidya等人報導,L1蛋白的表位 48EEYDLQFIFQLCKITLTA65, 45RHGEEYDLQFIFQLCKITLTA65, 63LPDPNKF69, 79PETQRLVWAC88, 36PVPGQYDA43, 77YNPETQRLVWAC88, 188DTGYGAMD195, 36PVPGQYDATK45, 45KQDIPKVSAYQYRVFRV61, 130RDNVSVDYKQTQLCI144 and 49YSRHVEEY DLQFIF62 可以用作設計HPV16和18型疫苗的工具(參見Epitope design of L1 protein for vaccine production against Human Papilloma Virus types 16 and 18,Bioinformation 13(3):86-93 March 2017,透過引用全部併入本文)。
Katharina Slupetzky等人報導HPV-16的 aa 282–286及351–355 附近的區域對於中和表位有貢獻,而且後者是免疫優勢位點(參見Chimeric papillomavirus-like particles expressing a foreign epitope on capsid surface loops,Journal of General Virology (2001), 82, 2799–2804,透過引用全部併入本文)。
Brooke Bishop等人製備了HPV11、16、18和35 L1蛋白的以下3種變體:其N端9個胺基酸缺失、α4(對應於HPV16的404–436位胺基酸殘基)缺失、其C端31個胺基酸缺失,報導前兩者不能組裝成VLP,但是未報導後者有此現象
(Crystal Structures of Four Types of Human Papillomavirus L1 Capsid Proteins UNDERSTANDING THE SPECIFICITY OF NEUTRALIZING MONOCLONAL ANTIBODIES,The Journal of Biological Chemistry,282,31803-31811。透過引用全部併入本文)。各型別的HPV L1蛋白的α螺旋、β折疊片各個Loop區都可以透過本領域常用的序列分析軟體方便地確定。其中α螺旋區包含α1區、α2區、α3區、α4區和α5區。
發明人對14種型別(6型、11型、16型、18型、31型、33型、35型、39型、45型、51型、52型、56型、58型和59型)的HPV L1蛋白進行序列比對,然後根據如上引用的文獻(Crystal Structures of Four Types of Human Papillomavirus L1 Capsid Proteins UNDERSTANDING THE SPECIFICITY OF NEUTRALIZING MONOCLONAL ANTIBODIES,The Journal of Biological Chemistry,282,31803-31811)進行二級結構預測,結果如下所示,其中向下的箭頭之間的部分對應於該文獻中關於的為製備變體而缺失的區域。
除發明人所用的序列對比的方法之外,可用於預測的蛋白質二級結構預測軟體包括但不限於:
1. JPred: http://www.compbio.dundee.ac.uk/jpred/index.html
2. ProtPredicct: http://predictprotein.org
3. PsiPred: http://bioinf.cs.ucl.ac.uk/psipred
4. SCRATCH-1D: http://download.igb.uci.edu
5. Nnpredict: http://www.cmpharm.ucsf.edu/~nomi/nnpredict
6. Predictprotein: http://www.embl-heidelberg.de/predictprotein/SOPMA http://www.ibcp.fr/predict.html
7. SSPRED: http://www.embl-heidelberg.de/sspred/ssprd_info.html。
在本發明的一個實施方式中,發明人以以下方式確定衍生於第一型別的HPV L1蛋白的N端片段的長度:將L1蛋白天然序列在其α5區及其附近區域截短,保留從其N末端至α5區域新產生的C末端的序列。如此截短的序列可以保證其具有本型別的免疫原性,且能夠形成VLP。
衍生於第一型別的HPV L1蛋白的N端片段還可以進一步改造,以保證其具有本型別的免疫原性,且能夠形成VLP為限。
發明人以以下方式確定了衍生於第二型別的HPV L1蛋白的C端片段的長度。將L1蛋白天然序列在其α5區及其附近區域截短、保留從其α5區域新產生的N末端至C末端序列。如此截短的序列不具有主要中和抗原表位,不干擾形成的嵌合蛋白的免疫原性。
衍生於第二型別的HPV L1蛋白的C端片段還可以進一步突變、缺失和/或添加,較佳保留其至少一個核定位序列。Yang 等人預測了107種HPV亞型的核定位序列(Yang et al. Predicting the nuclear localization signals of 107 types of HPV L1 proteins by bioinformatic analysis. Geno. Prot. Bioinfo. Vol. 4 No. 1 2006,透過引用全部併入本文),各型別的HPV L1蛋白的核定位序列可以透過本領域常用的序列分析軟體方便地確定。
上述N端片段和C端片段的連接發生在前者的新產生的C末端和後者的新產生的N末端。可以是直接連接也可以是透過連接子連接。將連接點視為原點,則在原點的N端側為負,而其C端側為正。
如下示出HPV6 L1蛋白的453-469位胺基酸序列、以及多個型別HPV L1蛋白的與之相對應的一段序列。可以看出這些序列高度相似。這段序列和α5區有重合。括號內數字表示所列出序列的最後一位胺基酸的位置,其中對於HPV 45型,一些HPV 45型毒株的L1蛋白的N端存在額外的26個胺基酸,而在另一些HPV 45型毒株的L1蛋白的N端不存在所述額外的26個胺基酸,所以以(478)+26表示。
HPV6 ELDQYPLGRKFLLQSGY(469)
HPV11 ELDQFPLGRKFLLQSGY(470)
HPV16 DLDQFPLGRKFLLQAGL(474)
HPV18 DLDQYPLGRKFLVQAGL(475)
HPV31 DLDQFPLGRKFLLQAGY(475)
HPV35 DLDQFPLGRKFLLQAGL(472)
HPV39 ELDQFPLGRKFLLQARV (474)
HPV45 DLDQYPLGRKFLVQAGL(478)+26
HPV51 DLDQFALGRKFLLQVGV(474)
HPV52 DLDQFPLGRKFLLQAGL(478)
HPV56 DLDQFPLGRKFLMQLGTRS(474)
HPV58 DLDQFPLGRKFLLQSGL(473)
HPV33 DLDQFPLGRKFLLQAGL(473) KAKPKLKRAAPTSTRTSSAKRKKVKK,其中,480-481的KR和493-496位的KRKK是核定位序列。
HPV59 DLDQFPLGRKFLLQLGA(475) RPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK,其中,484-486的RKR和498-504的KRVKRRK是核定位序列。
在本發明的一個實施方式中,發明人借助多個HPV型別之間的α5區及其附近區域的序列相似性,便利地完成了不同型別之間的L1蛋白的C端替換。
在本發明的最佳的實施方式中,發明人注意到各個型別的HPV L1蛋白都在相似的位置具有一段四肽RKFL,更有利的情形是一段六肽LGRKFL。發明人巧妙地利用這一高度保守的序列,將嵌合蛋白的連接點設計在這一段寡肽的任一胺基酸位點。自一個方面看來,自嵌合蛋白N末端起至RKFL或LGRKFL止與衍生於第一型別的HPV L1 蛋白的N端片段的序列相同,而從另一方面看來自RKFL或LGRKFL起至嵌合蛋白的C末端止,與衍生於第二型別的L1蛋白的C端片段的序列相同。
如此產生的嵌合蛋白保持與天然HPV L1蛋白高度相似性, 可以預期在生產乃至此後的醫療或預防過程中,都會有良好的表現。
本發明所屬技術領域中具有通常知識者會理解,因為同一型別的HPV有不同的毒株,因此其天然序列不同,利用不同毒株構建而成的嵌合蛋白亦落入本發明。
本發明所屬技術領域中具有通常知識者會理解,因為不同型別HPV L1的高度相似性,如果在嵌合蛋白構建過程中,將衍生於第一型別HPV L1蛋白的N端片段向C末端延伸更多的胺基酸殘基,或者是將衍生於第二型別的HPV L1蛋白的C端片段向N末端延伸更多的胺基酸殘基,亦有可能因相應位點上胺基酸的相同或相似,形成與本發明結構一致的嵌合蛋白。如此形成的嵌合蛋白亦落入本發明。
本發明所屬技術領域中具有通常知識者會理解,在以上描述的實施方式的嵌合蛋白的基礎上,會透過胺基酸殘基的突變、缺失和/或添加形成嵌合蛋白的變體。這些變體有可能具有第一型別的HPV L1蛋白的免疫原性、可以形成VLP,且具有良好的產量和可溶性。如此形成的嵌合蛋白亦落入本發明。
發明的有益效果
目前普遍用於生產類病毒顆粒的表達系統分為真核表達系統和原核表達系統。真核表達系統表達的乳頭瘤病毒L蛋白能自發裝配成類病毒顆粒,但具有表達量低不易規模化生產的缺點。原核表達系統表達的乳頭瘤病毒L蛋白的天然構型往往被破壞,需要後期進行體外處理才能得到類病毒顆粒,而且產量較低,很難進行產業化。
本發明將乳頭瘤病毒(例如人乳頭瘤病毒)L蛋白的C端進行改造,例如替換為HPV 16型L1蛋白、HPV 28型L1蛋白、HPV 33型L1蛋白、HPV 59型L1蛋白或HPV 68型L1蛋白中的C端片段,可以在表達系統(例如宿主細胞,例如昆蟲細胞)中提高乳頭瘤病毒L蛋白的表達量和可溶性。這可用於疫苗例如HPV疫苗的大規模生產。
發明人自行發現HPV 16型L1蛋白、HPV 28型L1蛋白、HPV 33型L1蛋白、HPV 59型L1蛋白和HPV 68型L1蛋白相較於其他型的L1蛋白表達量和可溶性較好,且發現所述增加的蛋白表達量和可溶性取決於所述HPV L1蛋白的C端序列。在107型HPV L1蛋白中,大部分在C端具有核定位序列,且C端序列具有一定的相似性。
對於目前無法表達、表達量非常低或表達後不可溶的乳頭瘤病毒L蛋白,將其C端片段替換為HPV 16型L1蛋白、HPV 28型L1蛋白、HPV 33型L1蛋白、HPV 59型L1蛋白或HPV 68型L1蛋白中的C端片段,使得原本表達量極低或不可溶的乳頭瘤L蛋白的可溶性表達和後續純化成為可能。這可以用於更多價疫苗(例如HPV疫苗)的大規模生產,使得更全面地預防多種乳頭瘤病毒,特別是HPV的感染成為可能。
為了實現疫苗的大規模生產,還存在提高HPV L1蛋白在昆蟲細胞中的表達量和可溶性的需求。此外,在酵母細胞中,因為無法正確形成雙硫鍵,HPV L1蛋白裝配成的類病毒顆粒缺乏良好的構型。
對於在昆蟲細胞中表達量低且可溶性差的HPV L1蛋白,將其C端片段改造為HPV 33型或59型L1蛋白的C端片段後,表達量和可溶性顯著提高,可用於HPV疫苗的大規模生產。
對於相較於其他型的L1蛋白在昆蟲細胞中表達量和可溶性較好的HPV L1蛋白,例如HPV 16型、HPV 28型L1蛋白、HPV 68型L1蛋白等,為了實現疫苗的大規模生產,還存在進一步提高表達量和可溶性的需求。在本發明中,例如,將HPV 16型L1蛋白的C端片段改造為HPV 33型L1蛋白的C端片段後,改造後的嵌合的HPV 16型L1蛋白表達量和可溶性均得到改善,有利於HPV疫苗的大規模生產。
總之,嵌合HPV L1蛋白相比於未改造之前的HPV L1蛋白在昆蟲細胞中的表達量和可溶性大大提高。可用於HPV疫苗的大規模生產。此外,嵌合HPV L1蛋白在昆蟲細胞中可以正確形成雙硫鍵而裝配為具有良好構型的HPV類病毒顆粒。這可以提高HPV類病毒顆粒的免疫原性,產生更好的免疫反應。
本發明的多價疫苗可以用於預防多種HPV相關疾病或感染,包括目前尚無法預防的HPV型別。
定義
除非另有說明,本文使用的所有技術和科學術語具有本發明所屬技術領域的通常知識者通常理解的含義。為方便地理解本發明,以下引述下列術語的通常含義。
當用於本文和所附申請專利範圍中時,單數形式「一個/種」、「另一個/種」和「所述/該」包括複數指代對象,除非上下文明確地另有指示。除非另有明確說明,否則術語「包括/包含/具有」、「例如」等旨在傳達包含而非限制。
術語「免疫原性」是指某種物質,例如蛋白質或多肽刺激免疫反應的能力,即刺激產生抗體,尤其是產生體液或者刺激細胞介導的反應的能力。
術語「抗體」指能結合抗原的免疫球蛋白分子。抗體可以是多株混合物或單株。抗體可以是源於天然來源或源於重組來源的完整的免疫球蛋白或可以是完整的免疫球蛋白的免疫反應性部分。抗體可以存在於多種形式,包括例如Fv、Fab’、F(ab’)2以及以單鏈存在。
術語「抗原性」是指某種物質,例如蛋白質或多肽產生與其特異性結合的抗體的能力。
術語「表位」包括能夠特異性結合至抗體或T細胞受體的任何蛋白質決定位。表位決定位通常由分子的化學活性表面基團(例如胺基酸或糖側鏈,或其組合)組成,並且通常具有特定三維結構特徵以及特定的電荷特徵。
術語「亞型」或「型別」可在本文中互換使用,表示所述病毒抗原的遺傳變體以使得一個亞型區別於一個不同亞型地被免疫系統識別。例如,HPV 16在免疫學上可區別於HPV 33。
術語「HPV L1蛋白」如本文所用,術語「HPV」和「人乳頭狀瘤病毒」是指乳頭狀瘤病毒科的無包膜雙鏈DNA病毒。它們的基因組是圓形的,並且大小約為8千鹼基對。大多數HPV編碼八種主要蛋白,六種位於「早期」區域(E1-E2),並且兩種位於「晚期」區域(L1(主要衣殼蛋白)和L2(次要衣殼蛋白))。已經鑒定了超過120種HPV類型,並且它們由數字標出(例如,HPV-16、HPV-18等)。
術語「HPV」或「HPV病毒」指乳頭狀瘤病毒科的乳頭狀瘤病毒,為無包膜DNA病毒,該病毒基因組為雙鏈閉環DNA,大小約為8 kb, 通常可以分為三個區域:①早期區(E),含有編碼E1、E2、E4~E7病毒複製,轉錄及轉譯有關的非結構蛋白的6個開放閱讀框,以及E3和E8開放閱讀框;②晚期區(L)含有編碼主要衣殼蛋白L1和次要衣殼蛋白L2的閱讀框;③長調控區(LCR)不編碼任何蛋白,但具有複製的起源以及多個轉錄因子結合位點。
術語「HPV L1蛋白」及「HPV L2蛋白」指由HPV基因的晚期區(L)編碼,在HPV感染週期中晚期合成的蛋白。L1蛋白質是主要的衣殼蛋白並且具有55‑60 kDa的分子量。L2蛋白質是次要的衣殼蛋白質。72個L1五聚體構成二十面體HPV病毒粒子的外殼,包裹閉環雙鏈DNA微染色體。L2蛋白質位於L1蛋白質內側。
術語「類病毒顆粒」是含有某種病毒的一個或多個結構蛋白的空心顆粒,沒有病毒核酸。
「HPV假病毒」係利用HPV VLP的非特異包裹核酸的特性,透過細胞內表達的HPV L1和L2組成的VLP包裹游離的DNA或導入外源質體形成。是理想的HPV體外中和實驗模型。
「假病毒中和法」是評價抗體的中和活性的一種方法,將免疫後的動物血清與一定量的假病毒孵育後再侵染細胞,細胞會隨著血清中中和抗體的增加而減少,在一定的範圍內可存在線性負相關,因此可以透過檢測表達細胞數的變化來評價血清中抗體的中和活性。
術語「其片段」或「其變體」指根據本發明的部分核苷酸或胺基酸序列被缺失、插入和/或取代。較佳地,本發明提供的多肽的片段或變體能在動物或人體中引發體液和/或細胞免疫反應。
術語「嵌合」意指,源自不同的親本分子的多肽或核苷酸序列分別經由醯胺鍵或3’,5’-磷酸二酯鍵連接在一起。較佳的,不被額外的連接子序列分隔開,而是直接彼此相鄰。
術語「截短」意指透過從多肽的N和/ 或C-末端除去一個或多個胺基酸或者缺失一個或多個多肽內部的胺基酸。
術語「核定位序列」為可引導蛋白質進入細胞核的胺基酸序列。在一些HPV L1蛋白中,兩個緊密的鹼性殘基位(即核定位序列)(例如一個是KRKR、KRKK、KRKRK、KRKKRK、KRVKRRK等,另一個是KR、RKR、KRK等)之間具有10-14個胺基酸的間隔區。上述鹼性殘基位屬於核定位序列。在另一些HPV L1蛋白中,核定位序列為精胺酸和/或賴胺酸形成的緊密的鹼性殘基位。核定位序列包括但不限於如上所述鹼性殘基位的實例。參見Jun Yang等,Predicting the Nuclear Localization Signals of 107 Types of HPV L1 Proteins by Bioinformatic Analysis,Genomics, Proteomics & Bioinformatics Volume 4, Issue 1, 2006, Pages 34-41,其全部內容透過引用併入本文。
術語「功能性變體」為某一多肽或蛋白經截短、突變、缺失和/或添加後仍然保持所需要的活性或特徵的版本。
兩條多肽或核酸序列之間的「序列同一性」表示所述序列之間相同的殘基的數目占殘基總數的百分比,且基於比較的分子中較小者的大小來計算。在計算同一性百分數時,將正在比較的序列以產生序列之間最大匹配的方式比對,透過特定算法解決比對中的空位(如果存在的話)。確定兩個序列之間同一性的較佳電腦程式方法包括,但不限於,GCG程序包,包括GAP、BLASTP、BLASTN和FASTA(Altschul等人,1990,J.Mol.Biol.215:403-410)。上述程式可以公開地從國際生物技術信息中心(NCBI)和其他來源得到。習知的Smith Waterman算法也可用於確定同一性。
可以保守性置換非關鍵的胺基酸而不影響蛋白質的正常功能。保守性置換意指用化學或功能相似的胺基酸置換胺基酸。提供相似胺基酸的保守性置換表是本領域熟知的。舉例來說,在一些實施方式中,表1-3中提供的胺基酸組被認為是相互的保守性置換。
表1 在某些實施方式中,被認為是相互保守性置換的胺基酸的所選組
酸性殘基 | D和E |
鹼性殘基 | K、R和H |
親水性不帶電荷的殘基 | S、T、N和Q |
脂肪族不帶電荷的殘基 | G、A、V、L和I |
非極性不帶電荷的殘基 | C、M和P |
芳香族殘基 | F、Y和W |
表2 在某些實施方式中,被認為是相互的保守性置換的胺基酸的其他所選組
組 1 | A、S和T |
組 2 | D和E |
組 3 | N和Q |
組 4 | R和K |
組 5 | I、L和M |
組 6 | F、Y和W |
表3 在某些實施方式中,被認為是相互的保守性置換的胺基酸的其他所選組
組 A | A和G |
組 B | D和E |
組 C | N和Q |
組 D | R、K和H |
組 E | I、L、M、V |
組 F | F、Y和W |
組 G | S和T |
組 H | C和M |
術語「胺基酸」意指二十種常見的天然存在的胺基酸。天然存在的胺基酸包括丙胺酸(Ala;A)、精胺酸(Arg;R)、天冬醯胺酸(Asn;N)、天冬胺酸(Asp;D)、胱胺酸(Cys;C);麩胺酸(Glu;E)、麩醯胺酸(Gln;Q)、甘胺酸(Gly;G)、組胺酸(His;H)、異白胺酸(Ile;I)、白胺酸(Leu;L)、離胺酸(Lys;K)、甲基胺酸(Met;M)、苯丙胺酸(Phe;F)、脯胺酸(Pro;P)、絲胺酸(Ser;S)、蘇胺酸(Thr;T)、色胺酸(Trp;W)、酪胺酸(Tyr;Y)和纈胺酸(Val;V)。
如本文所用的「生理學上可接受的載體」,其對所用劑量和濃度的細胞或哺乳動物是無毒的。通常為pH緩衝含水溶液,其非限制性實例包括緩衝劑;抗氧化劑;寡肽;蛋白質;親水性聚合物;胺基酸;單糖、二糖和其它碳水化合物;螯合劑;糖醇;成鹽的抗衡離子,例如鈉;以及/或非離子表面活性劑。
術語「佐劑」指一種增強免疫反應的化合物或混合物。特別的,疫苗可以包含佐劑。用於本發明的佐劑可以包括但不限於以下的一種或多種:含礦物佐劑組合物、油-乳佐劑、皂素佐劑製劑、細菌或微生物衍生物。
術語「載體」意指能夠增殖與其連接的另一核酸的核酸分子。該術語包括作為自我複製核酸結構的載體以及作為整合至已引入載體的宿主細胞基因組中的載體。某些載體能夠引導該類載體以可操作方式連接的核酸的表達。
術語「宿主細胞」意指已引入外源核酸的細胞,以及這樣的細胞的後代。宿主細胞包括「轉化體」(或「轉化細胞」)、「轉染體」(或「轉染細胞」)或「感染體」(或「感染細胞」),其各自包括初代轉化、轉染或感染的細胞和由其衍生的後代。這樣的後代在核酸含量上可能不與親本細胞完全相同,並且可能含有突變。
施用量較佳的為「預防性有效量」(本文預防可以被認為是治療,兩者可互換使用),其足以對個體顯示出益處。
實施例
實施例1 基因的構建
實施例1.1:HPV6L1 C端替換為HPV33L1 C端的嵌合基因的構建
1.1.1 用作模板的pFB-HPV6L1的構建
委託Thermo Fisher公司(原英濰捷基(上海)貿易有限公司)基因合成HPV6L1基因,且合成的序列兩端分別具有KpnⅠ和XbaI酶切位點,其序列見SEQ ID NO: 5。透過KpnⅠ和XbaI酶切位點將合成的基因片段與pcDNA3載體(銷售商Thermo Fisher)連接,得到含有編碼HPV6L1 1-500個胺基酸的核苷酸序列的質體pcDNA3-HPV6-L1。
將得到的pcDNA3-HPV6-L1質體進行KpnⅠ和XbaI雙酶切得到HPV6L1(1-500)的基因的片段。再將該片段與KpnⅠ和XbaI雙酶切的pFastBacTM1載體(銷售商Thermo Fisher)進行連接,得到含HPV6L1(1-500)基因片段的桿粒載體,命名為pFB-HPV6L1。
1.1.2 用作模板的pFB-HPV33L1的構建
委託Thermo Fisher公司(原英濰捷基(上海)貿易有限公司)基因合成HPV33L1基因,且合成序列兩端分別具有KpnI和XbaI酶切位點,基因片段序列見SEQ ID NO: 6。透過KpnI和XbaI酶切位點將合成的基因片段與pcDNA3載體(銷售商Thermo Fisher)連接,得到含有編碼HPV33L1 1-499位胺基酸的核苷酸序列的質體pcDNA3-HPV33-L1。
將得到的pcDNA3-HPV33-L1質體進行KpnI和XbaI雙酶切得到HPV33L1(1-499)的基因的片段。再將該片段與KpnI和XbaI雙酶切的pFastBacTM1載體(銷售商Thermo Fisher)進行連接,得到含HPV33L1(1-499)基因片段的桿粒載體,命名為pFB-HPV33L1。
1.1.3 pFB-HPV6L1:33C的構建
HPV6L1 C端替換為HPV33L1 C端的嵌合基因:以構建成功的重組質體pFB-HPV6L1為基因模板,用引子F1和R1擴增長度為1426bp基因片段,引子序列F1如SEQ ID No: 7所示,R1如SEQ ID No: 8所示。
該基因片段包含編碼HPV6L1的1-469胺基酸的基因片段、與HPV33L1的474-499胺基酸的基因片段重疊的10個鹼基以及KpnI酶切位點(GGTAC^C)段,擴增的序列如SEQ ID No: 9所示:
PCR擴增參數:94℃預變性 5min;98℃變性 10s、69℃退火 15s、72℃ 1kb/1min、進行30個循環;72℃延伸 5min;16℃結束。
以重組質體pFB-HPV33L1為基因模板,用引子F2和R2,擴增長度101 bp的基因片段,引子序列F2如SEQ ID No: 10所示,R2如SEQ ID No: 11所示。
該基因片段含HPV33L1 C端的26個(474-499)胺基酸的基因片段、與HPV6L1的1-469胺基酸C端基因片段重疊的10bp鹼基以及XbaI(T^CTAGA)酶切位點,擴增的序列如SEQ ID No: 12所示。
PCR擴增參數:94℃預變性 5min;98℃變性 10s、69℃退火 15s、72℃ 1kb/1min、進行 30個循環;72℃延伸 5min;16℃結束。
PCR拼接序列:
拼接引子分別為F1和R2,以上述引子擴增得到的片段(F1和R1擴增得到的片段,F2和R2擴增得到的片段)為模板。
PCR拼接參數:94℃預變性 5min;98℃變性 10s、52℃退火 15s、72℃ 1kb/1min、進行 5個循環;98℃變性 10s、68℃退火 15s、72℃ 1kb/1min、進行 25個循環;72℃延伸 5min;16℃結束。
最終得到SEQ ID NO: 4,編碼由HPV6L1的1-469胺基酸和HPV33L1 C端的26個(474-499)胺基酸組成的核苷酸序列,兩端帶有KpnI和XbaI酶切位點(下稱拼接序列)。
用KpnI+XbaI雙酶切pFastBacTM1載體和拼接序列片段,將拼接序列選殖到pFastBacTM1載體上,獲得重組質體pFB-HPV6L1:33C。即為HPV6L1 C端替換為HPV33L1 C端的嵌合基因。
實施例1.2 HPV11L1 C端替換為HPV33L1 C端的嵌合基因的構建
實驗方法和步驟與實施例1.1相同,相關序列參見附錄2。
實施例1.3 HPV16L1 C端替換為HPV33L1 C端的嵌合基因的構建
實驗方法和步驟與實施例1.1相同,相關序列參見附錄3。
實施例1.4 HPV18L1 C端替換為HPV33L1 C端的嵌合基因的構建
實驗方法和步驟與實施例1.1相同,相關序列參見附錄4。
實施例1.5 HPV31L1 C端替換為HPV33L1 C端的嵌合基因的構建
實驗方法和步驟與實施例1.1相同,相關序列參見附錄5。
實施例1.6 HPV33L1基因的構建
1.6.1 pFB-HPV33L1基因的製備
HPV33L1基因透過基因合成的方法構建而成,委託Thermo Fisher公司(原英濰捷基(上海)貿易有限公司)基因合成,合成序列兩端分別具有KpnⅠ和XbaI酶切位點,基因片段序列見SEQ ID No: 68。透過KpnⅠ和XbaI酶切位點將合成的基因片段與pcDNA3載體(銷售商Thermofisher)連接,得到含有編碼HPV33L1 1-499個胺基酸的核苷酸序列的質體pcDNA3-HPV33-L1。
將得到的pcDNA3-HPV33-L1質體進行KpnⅠ和XbaI雙酶切得到HPV33L1 (1-499)的基因的片段。再將該片段與KpnⅠ和XbaI雙酶切的pFastBacTM1載體(銷售商Thermofisher)進行連接,得到含HPV33L1 (1-499)基因片段的桿粒載體,命名為pFB-HPV33L1。
實施例1.7 HPV35L1 C端替換為HPV33L1 C端的嵌合基因的構建
實驗方法和步驟與實施例1.1相同,相關序列參見附錄7。
實施例1.8 HPV39L1 C端替換為HPV59L1 C端的嵌合基因的構建
1.8.1 用作模板的pFB-HPV39L1的構建
委託Thermo Fisher公司(原英濰捷基(上海)貿易有限公司)基因合成HPV39L1基因,且合成的序列兩端分別具有KpnⅠ和XbaI酶切位點,其序列見SEQ ID NO: 86。透過KpnⅠ和XbaI酶切位點將合成的基因片段與pcDNA3載體(銷售商Thermo Fisher)連接,得到含有編碼HPV39L1 1-505個胺基酸的核苷酸序列的質體pcDNA3-HPV39-L1。
將得到的pcDNA3-HPV39-L1質體進行KpnⅠ和XbaI雙酶切得到HPV39L1(1-505)的基因的片段。再將該片段與KpnⅠ和XbaI雙酶切的pFastBacTM1載體(銷售商Thermo Fisher)進行連接,得到含HPV39L1(1-505)基因片段的桿粒載體,命名為pFB-HPV39L1。
1.8.2 用作模板的pFB-HPV59L1的構建
委託Thermo Fisher公司(原英濰捷基(上海)貿易有限公司)基因合成HPV59L1基因,且合成序列兩端分別具有KpnI和XbaI酶切位點,基因片段序列見SEQ ID NO: 87。透過KpnI和XbaI酶切位點將合成的基因片段與pcDNA3載體(銷售商Thermo Fisher)連接,得到含有編碼HPV59L1 1-508位胺基酸的核苷酸序列的質體pcDNA3-HPV59-L1。
將得到的pcDNA3-HPV59-L1質體進行KpnI和XbaI雙酶切得到HPV59L1(1-508)的基因的片段。再將該片段與KpnI和XbaI雙酶切的pFastBacTM1載體(銷售商Thermo Fisher)進行連接,得到含HPV59L1(1-508)基因片段的桿粒載體,命名為pFB-HPV59L1。
1.8.3 pFB-HPV39L1:59C的構建
HPV39L1 C端替換為HPV59L1 C端的嵌合基因:以構建成功的重組質體pFB-HPV39L1為基因模板,用引子F1和R1擴增長度為1428 bp基因片段,引子序列F1如SEQ ID No: 88所示,R1如SEQ ID No: 89所示。
該基因片段包含編碼HPV39L1的1-469胺基酸的基因片段、與HPV59L1的471-508胺基酸的基因片段重疊的12個鹼基以及KpnI酶切位點(GGTAC^C)段,擴增的序列如SEQ ID No: 90所示:
PCR擴增參數:94℃預變性 5min;98℃變性 10s、69℃退火 15s、72℃ 1kb/1min、進行30個循環;72℃延伸 5min;16℃結束。
以重組質體pFB-HPV59L1為基因模板,用引子F2和R2,擴增長度139bp的基因片段,引子序列F2如SEQ ID No: 91所示,R2如SEQ ID No: 92所示。
該基因片段含HPV59L1 C端的38個(471-508)胺基酸的基因片段、與HPV39L1的1-469胺基酸C端基因片段重疊的12bp鹼基以及XbaI(T^CTAGA)酶切位點,擴增的序列如SEQ ID No: 93所示。
PCR擴增參數:94℃預變性 5min;98℃變性 10s、69℃退火 15s、72℃ 1kb/1min、進行 30個循環;72℃延伸 5min;16℃結束。
PCR拼接序列:
拼接引子分別為F1和R2,以上述引子擴增得到的片段(F1和R1擴增得到的片段,F2和R2擴增得到的片段)為模板。
PCR拼接參數:94℃預變性 5min;98℃變性 10s、52℃退火 15s、72℃ 1kb/1min、進行 5個循環;98℃變性 10s、68℃退火 15s、72℃ 1kb/1min、進行 25個循環;72℃延伸 5min;16℃結束。
最終得到SEQ ID NO: 85,編碼由HPV39L1的1-469胺基酸和HPV59L1 C端的38個(471-508)胺基酸組成的核苷酸序列,兩端帶有KpnI和XbaI酶切位點(下稱拼接序列)。
用KpnI+XbaI雙酶切pFastBacTM1載體和拼接序列片段,將拼接序列克隆到pFastBacTM1載體上,獲得重組質體pFB-HPV39L1:59C。即為HPV39L1 C端替換為HPV59L1 C端的嵌合基因。
實施例1.9 HPV45L1 C端替換為HPV33L1 C端的嵌合基因的構建
實驗方法和步驟與實施例1.1相同,相關序列參見附錄9。
實施例1.10 HPV51L1 C端替換為HPV33L1 C端的嵌合基因的構建
實驗方法和步驟與實施例1.1相同,相關序列參見附錄10。
實施例1.11 HPV52L1 C端替換為HPV33L1 C端的嵌合基因的構建
實驗方法和步驟與實施例1.1相同,相關序列參見附錄11。
實施例1.12 HPV56L1 C端替換為HPV33L1 C端的嵌合基因的構建
實驗方法和步驟與實施例1.1相同,相關序列參見附錄12。
實施例1.13 HPV58L1 C端替換為HPV33L1 C端的嵌合基因的構建
實驗方法和步驟與實施例1.1相同,相關序列參見附錄13。
實施例1.14 HPV59L1基因的構建
實施例1.14.1 pFB-HPV59L1基因的製備
HPV59L1基因透過基因合成的方法構建而成,委託Thermo Fisher公司(原英濰捷基(上海)貿易有限公司)基因合成,合成序列兩端分別具有KpnⅠ和XbaI酶切位點,基因片段序列見SEQ ID No: 162。透過KpnⅠ和XbaI酶切位點將合成的基因片段與pcDNA3載體(銷售商Thermofisher)連接,得到含有編碼HPV59L1 1-508個胺基酸的核苷酸序列的質體pcDNA3-HPV59-L1。
將得到的pcDNA3-HPV59-L1質體進行KpnⅠ和XbaI雙酶切得到HPV59L1 (1-508)的基因的片段。再將該片段與KpnⅠ和XbaI雙酶切的pFastBacTM1載體(銷售商Thermofisher)進行連接,得到含HPV59L1 (1-508)基因片段的桿粒載體,命名為pFB-HPV59L1。
實施例2 重組桿狀病毒的包裝
實施例2.1:HPV 6L1:33C重組桿狀病毒包裝
實施例1構建的pFB-HPV 6L1:33C的重組質體經鑒定和測序正確後,將其轉化至DH10Bac細菌感受態細胞(Bac-to-Bac®試劑盒,購於Thermo Fisher)中,37℃培養擴增,並進行平盤劃線培養,挑選白色菌斑並擴增,培養過夜後收集菌液,使用鹼裂解法提取重組桿粒DNA。
用陽離子轉染試劑(購於Sino Biological)將其轉染至昆蟲細胞SF9中進行重組桿狀病毒毒種包裝。具體操作如下:
a. 取對數生長期的SF9細胞按照0.6×10
6cell/dish的密度接種dish,將接種有SF9細胞的dish室溫放置2h,貼壁。
b. 提取的質體20μL Bacmid DNA加至200μL Grace’s Medium(無血清,無添加物,購於Gibico)混合顛倒5次。
c. 25 μL 0.2x TF1(轉染試劑,購於Sino Biological)滴加至200 μL Grace’s Meduim輕輕混勻。
d. 將b和c混合。室溫孵育15-45min。
e. 當DNA與cellfectin(購於Sino Biological)孵育時,棄細胞上清,添加無血清添加物的Grace Medium 0.8mL/dish。
f. 將d中孵育好的DNA與轉染試劑複合物滴加到dish中。
g. 27℃孵育2hr。
h. 丟棄細胞培養液,加2.5 mL/dish完全生長培養基(SCD6 SF +10%FBS)(SCD6 SF購於Sino Biological,FBS購於Gibico)。
i. 27℃培養7天觀察是否有病毒感染。
轉染後待細胞產生明顯的病變後收集病毒上清,一般培養7-11天。用移液器無菌收取病毒上清液,即為HPV6L1:33C P1代毒種。使用HPV6L1:33C P1代毒種按照1:50(V/V)比例感染SF9細胞,SF9細胞的感染密度為2×10
6cells/mL,27℃培養擴增3天,1000g±200g室溫離心10min,收集的病毒上清液即為P2代病毒,可用於感染生產。
實施例2.2:HPV 11L1:33C重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例2.3:HPV 16L1:33C重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例2.4:HPV 18L1:33C重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例2.5:HPV 31L1:33C重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例2.6:HPV 33L1重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例2.7:HPV 35L1:33C重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例2.8:HPV 39L1:59C重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例2.9:HPV 45L1:33C重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例2.10:HPV 51L1:33C重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例2.11:HPV 52L1:33C重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例2.12:HPV 56L1:33C重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例2.13:HPV 58L1:33C重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例2.14:HPV 59L1重組桿狀病毒包裝
實驗方法和步驟與實施例2.1相同。
實施例3 嵌合蛋白或蛋白的表達
實施例3.1:HPV 6L1:33C表達生產
用實施例2中獲得的含有HPV 6L1:33C 重組基因的桿狀病毒感染High Five細胞,感染比例1:200(V/V),1000g±100g室溫離心收集細胞沉澱,使用PBS或MOPS 緩衝液(pH6.0-7.0,鹽濃度100 mM-1M)超音波裂解細胞沉澱,低溫超音波破碎3min,大於10000g的離心力離心10分鐘,收集離心後上清液,SDS-PAGE電泳檢測。泳道1: Marker(Marker為7種純化後的蛋白,分子量大小包含 14.4 至116kDa,生產商為Thermo Scientific);泳道2:細胞裂解液;泳道3:裂解液離心後收集的上清液。
結果如圖1A所示,該方法製備的HPV 6L1:33C L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.2:HPV 11L1:33C表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1B所示,該方法製備的HPV 11L1:33C L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.3:HPV 16L1:33C表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1C所示,該方法製備的HPV 16L1:33C L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.4:HPV 18L1:33C表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1D所示,該方法製備的HPV 18L1:33C L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.5:HPV 31L1:33C表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1E所示,該方法製備的HPV 31L1:33C L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.6:HPV 33L1表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1F所示,該方法製備的HPV 33L1 L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.7:HPV 35L1:33C表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1G所示,該方法製備的HPV 35L1:33C L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.8:HPV 39L1:59C表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1H所示,該方法製備的HPV 39L1:59C L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.9:HPV 45L1:33C表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1I所示,該方法製備的HPV 45L1:33C L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.10:HPV 51L1:33C表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1J所示,該方法製備的HPV 51L1:33C L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.11:HPV 52L1:33C表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1K所示,該方法製備的HPV 52L1:33C L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.12:HPV 56L1:33C表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1L所示,該方法製備的HPV 56L1:33C L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.13:HPV 58L1:33C表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1M所示,該方法製備的HPV 58L1:33C L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例3.14:HPV 59L1表達生產
實驗方法和步驟與實施例3.1相同。
結果如圖1N所示,該方法製備的HPV 59L1 L1蛋白產量大於100 mg/L,蛋白大小約56 KD,可以用於大規模生產。
實施例4 類病毒顆粒的純化製備
實施例4.1:HPV 6L1:33C類病毒顆粒的純化製備
HPV 6L1:33C類病毒顆粒純化方法為兩步層析法,即HS-MMA法,純化實施例3中收集的上清液,最終可得到高純度的類病毒顆粒。
第一步層析:
介質:採用Thermo Fisher公司生產的POROS
®50 HS強陽離子交換介質。
介質體積:介質體積150mL,線性流速30mL/min。
層析條件:平衡緩衝液(pH6.2,鹽濃度為50mM磷酸鹽,0.5M氯化鈉);清洗緩衝液(鹽濃度為50mM磷酸鹽,0.75M氯化鈉,pH6.2;)
層析柱先用 5 CV平衡緩衝液,然後上樣。上樣結束後,之後分別用5 CV的平衡緩衝液和清洗緩衝液洗脫雜蛋白。
洗脫條件:pH6.2,洗脫鹽濃度為1.25M 氯化鈉採用含有50mM 鹽酸精胺酸的50mM磷酸鹽緩衝液進行洗脫。
第二步層析:
介質:採用上海博格隆公司生產的MMA離子交換介質。
介質體積:介質體積150mL,線性流速30mL/min。
層析條件:平衡緩衝液50mM PB,1.25M NaCl, pH6.2。層析柱先用4CV平衡緩衝液平衡,然後上樣。上樣結束後,用5 CV 平衡緩衝液沖洗雜蛋白後,然後用洗脫緩衝液洗脫目標蛋白收集蛋白。
洗脫條件:100mM NaAC,150mM NaCl,0.01% Tween 80, pH4.5。
實施例4.2:HPV 11L1:33C類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例4.3:HPV 16L1:33C類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例4.4:HPV 18L1:33C類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例4.5:HPV 31L1:33C類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例4.6:HPV 33L1類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例4.7:HPV 35L1:33C類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例4.8:HPV 39L1:59C類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例4.9:HPV 45L1:33C類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例4.10:HPV 51L1:33C類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例4.11:HPV 52L1:33C類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例4.12:HPV 56L1:33C類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例4.13:HPV 58L1:33C類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例4.14:HPV 59L1類病毒顆粒的純化製備
實驗方法和步驟與實施例4.1相同。
實施例5 類病毒顆粒的形態學檢測
實施例5.1:HPV 6L1:33C類病毒顆粒的形態學檢測
取10μL樣品用於穿透式電子顯微鏡觀察。將樣品固定到碳噴銅網上吸附2min,殘餘液體用濾紙吸掉,再使用磷鎢酸(北京中鏡科儀技術有限公司,濃度2%,pH6.5)染色兩次,每次30秒,殘餘染色液用濾紙吸掉,晾乾後即可在穿透式電子顯微鏡下觀察。穿透式電子顯微鏡(品牌:日立,型號:H-7650)為80KV,放大倍數為80,000倍。
電子顯微鏡觀察結果見圖2A,由圖2A可見,C端改造的HPV 6L1:33C可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.2:HPV 11L1:33C類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2B,由圖2B可見,C端改造的HPV 11L1:33C可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.3:HPV 16L1:33C類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2C,由圖2C可見,C端改造的HPV 16L1:33C可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.4:HPV 18L1:33C類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2D,由圖2D可見,C端改造的HPV 18L1:33C可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.5:HPV 31L1:33C類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2E,由圖2E可見,C端改造的HPV 31L1:33C可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.6:HPV 33L1類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2F,由圖2F可見,C端改造的HPV 33L1可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.7:HPV 35L1:33C類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2G,由圖2G可見,C端改造的HPV 35L1:33C可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.8:HPV 39L1:59C類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2H,由圖2H可見,C端改造的HPV 39L1:59C可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.9:HPV 45L1:33C類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2I,由圖2I可見,C端改造的HPV 45L1:33C可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.10:HPV 51L1:33C類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2J,由圖2J可見,C端改造的HPV 51L1:33C可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.11:HPV 52L1:33C類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2K,由圖2K可見,C端改造的HPV 52L1:33C可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.12:HPV 56L1:33C類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2L,由圖2L可見,C端改造的HPV 56L1:33C可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.13:HPV 58L1:33C類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2M,由圖2M可見,C端改造的HPV 58L1:33C可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例5.14:HPV 59L1類病毒顆粒的形態學檢測
實驗方法和步驟與實施例5.1相同。
電子顯微鏡觀察結果見圖2N,由圖2N可見,C端改造的HPV 59L1可以形成大小均一的類病毒顆粒,平均直徑在60nm左右。
實施例6 類病毒顆粒動物免疫原性評價
實施例6.1:HPV 6L1:33C類病毒顆粒動物免疫原性評價
6.1.1 假病毒中和細胞的模型建立
由於HPV很難進行體外培養,又具有較強的宿主特異性,很難在除人體以外的生物體進行繁殖,缺乏合適的動物模型。所以需要建立合適有效的體外中和實驗模型,用於疫苗免疫保護性的評估。
HPV假病毒是理想的HPV體外中和實驗模型:利用HPV VLP具有非特異包裹核酸的特性,細胞內表達的HPV L1和L2組成的VLP包裹游離的DNA或導入外源質體形成HPV假病毒。
採用假病毒中和法對樣品免疫後動物血清樣品進行免疫原性分析。HPV6類病毒顆粒樣品免疫動物後能產生針對HPV6的中和抗體,能中和HPV6型的假病毒。將免疫後的動物血清與一定量的假病毒孵育後再侵染細胞,可表達GFP螢光的細胞會隨著血清中中和抗體的增加而減少,在一定的範圍內可存在線性負相關,因此可以透過檢測表達GFP的細胞數的變化來評價血清中抗體的中和活性。
假病毒構建方法:將HPV6型的 pCMV3-3-HPV6L1+L2(L1序列來源於Uniprot P69898,L2序列來源Uniprot Q84297) 質體(購於Sino Biological)以及螢光質體(PSEU-GFP Spark,購於Sino Biological)共轉染至293FT貼壁細胞(購於Thermo Fisher)。具體方法參考文獻(Pastrana D V, Buck C B, Pang Y S, Thompson C D, Castle P E, FitzGerald P C, Kjaer S K, Lowy D R, Schiller J T.Reactivity of human sera in a sensitive, high-throughput pseudovirus-based papillomavirus neutralization assay for HPV16 and HPV18. J Virology 2004,321:205-216.)。收集假病毒上清液並進行分裝,置於-80℃冰箱中保存備用。
6.1.2 HPV 6L1:33C類病毒顆粒動物免疫保護性評價
小鼠免疫程序:
HPV 6L1:33C類病毒顆粒吸附於磷酸鋁佐劑上,經混合後取200µL用於免疫小鼠,每隻小鼠免疫劑量0.15µg,免疫10隻小鼠,於實驗第0天、第7天、第21天分別用稀釋後樣品對小鼠進行免疫,同時設立空白血清對照組,於實驗第28天摘取小鼠眼球取血,分離出血清進行假病毒中和效價檢測。
小鼠EC
50檢測:
小鼠血清在56℃滅活30分鐘後,離心6000g,5分鐘後取上清進行檢測。檢測前4-8小時,將293FT細胞以15000細胞/孔的密度鋪板於96孔板中,培養於37℃,5%CO
2的二氧化碳培養箱中。免疫後小鼠血清、空白對照血清均用中和培養基系列稀釋後按照體積比1:1分別與6.1中製備的HPV6假病毒混合。2~8℃冰箱中孵育1小時後按照100μL/孔加入到提前4-8小時鋪板的293FT細胞上,每個樣品2個複孔,同時設立空白血清對照孔、假病毒陽性對照孔和陰性對照孔。加樣後的細胞繼續在37℃,5%CO
2的二氧化碳培養箱中培養62-96小時後,在酶聯斑點分析儀中(型號:S6 Universal-V Analyzer,廠家:CTL)進行螢光掃描拍照以及計數。透過計算每個小鼠血清樣品的中和抑制率,依據Reed-Muench法計算得到血清中和抑制率為50%時血清最大稀釋倍數,即半數有效稀釋倍數EC
50。
HPV6血清假病毒中和效價檢測結果詳見表4。
表 4 小鼠血清中和效價檢測結果 EC
50 ( GMT±SEM )
備註:
1. 動物數,N=10;
2. GMT(Geometric Mean Titer):幾何平均效價;
3. SEM(Standard Error of Mean):標準誤差。
HPV型 | EC 50值 |
6型 | 4948±1831 |
上述檢測結果顯示,本發明製備的HPV 6L1:33C類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.2:HPV 11L1:33C類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot P04012,L2序列來源Uniprot P04013。
HPV11血清假病毒中和效價檢測結果詳見表5。
表
5
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
HPV型 | EC 50值 |
11型 | 15024±8400 |
上述檢測結果顯示,本發明製備的HPV 11L1:33C類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.3:HPV 16L1:33C類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot P03101,L2序列來源Uniprot P03107。
HPV16血清假病毒中和效價檢測結果詳見表6。
表
6
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
HPV型 | EC 50值 |
16型 | 32449±7224 |
上述檢測結果顯示,本發明製備的HPV 16L1:33C類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.4:HPV 18L1:33C類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot Q80B70,L2序列來源Uniprot P06793。
HPV18血清假病毒中和效價檢測結果詳見表7。
表
7
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
HPV型 | EC 50值 |
18型 | 18480±4051 |
上述檢測結果顯示,本發明製備的HPV 18L1:33C類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.5:HPV 31L1:33C類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot P17388,L2序列來源Uniprot P17389。
HPV31血清假病毒中和效價檢測結果詳見表8。
表
8
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
HPV型 | EC 50值 |
31型 | 5210±1147 |
上述檢測結果顯示,本發明製備的HPV 31L1:33C類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.6:HPV 33L1類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot P06416,L2序列來源Uniprot P06418。
HPV33血清假病毒中和效價檢測結果詳見表9。
表
9
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
上述檢測結果顯示,本發明製備的HPV 33L1類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
HPV型 | EC 50值 |
33型 | 4666±1555 |
實施例6.7:HPV 35L1:33C類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot P27232,L2序列來源Uniprot P27234。
HPV35血清假病毒中和效價檢測結果詳見表10。
表
10
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
HPV型 | EC 50值 |
35型 | 2293±1448 |
上述檢測結果顯示,本發明製備的HPV 35L1:33C類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.8:HPV 39L1:59C類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot P24838,L2序列來源Uniprot P24839。
HPV39血清假病毒中和效價檢測結果詳見表11。
表
11
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
HPV型 | EC 50值 |
39型 | 25526±5857 |
上述檢測結果顯示,本發明製備的HPV 39L1:59C類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.9:HPV 45L1:33C類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot P36741,L2序列來源Uniprot P36761。
HPV45血清假病毒中和效價檢測結果詳見表12。
表
12
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
HPV型 | EC 50值 |
45型 | 755+935 |
上述檢測結果顯示,本發明製備的HPV 45L1:33C類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.10:HPV 51L1:33C類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot P26536,L2序列來源Uniprot P26539 。
HPV51血清假病毒中和效價檢測結果詳見表13。
表
13
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
HPV型 | EC 50值 |
51型 | 5528±1572 |
上述檢測結果顯示,本發明製備的HPV 51L1:33C類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.11:HPV 52L1:33C類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot Q05138,L2序列來源Uniprot F8S4U2。
HPV52血清假病毒中和效價檢測結果詳見表14。
表
14
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
HPV型 | EC 50值 |
52型 | 19019±8604 |
上述檢測結果顯示,本發明製備的HPV 52L1:33C類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.12:HPV 56L1:33C類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot P36743,L2序列來源Uniprot P36765。
HPV56血清假病毒中和效價檢測結果詳見表15。
表
15
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
HPV型 | EC 50值 |
56型 | 2497±612 |
上述檢測結果顯示,本發明製備的HPV 56L1:33C類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.13:HPV 58L1:33C類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot P26535,L2序列來源Uniprot B6ZB12。
HPV58血清假病毒中和效價檢測結果詳見表16。
表
16
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
HPV型 | EC 50值 |
58型 | 19939±8459 |
上述檢測結果顯示,本發明製備的HPV 58L1:33C類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.14:HPV 59L1類病毒顆粒動物免疫原性評價
實驗方法和步驟與實施例6.1相同。L1序列來源於Uniprot Q81971,L2序列來源Uniprot Q81970。
HPV59血清假病毒中和效價檢測結果詳見表17。
表
17
小鼠血清中和效價檢測結果
EC
50
(
GMT±SEM
)
HPV型 | EC 50值 |
59型 | 1344±335 |
上述檢測結果顯示,本發明製備的HPV 59L1類病毒顆粒具有較好的免疫原性,可在動物體內產生高效價的中和抗體,可以用於製備成預防HPV感染的疫苗。
實施例6.15:14價類病毒顆粒免疫組合物動物免疫原性評價
小鼠免疫程序
將如上所述的14型(HPV 6、11、16、18、31、33、35、39、45、51、52、56、58、59)類病毒顆粒用磷酸鋁空白佐劑在無菌條件下系列稀釋後(以樣品中單個劑量為20 μg的HPV型為標準進行稀釋,將該型樣品稀釋80倍至0.5 μg/mL,其他型樣品的稀釋則隨著各型配比組分的變化而變化),取200 μL用於免疫小鼠,每隻小鼠免疫劑量見表18。將6~8週齡 SPF級雌性Balb/c小鼠按照每組10隻小鼠進行分組(實驗組和對照),於實驗第0天、第7天、第21天分別用稀釋後樣品對各組小鼠進行免疫,樣品免疫一組小鼠,同時設立空白血清對照組,於實驗第28天摘取小鼠眼球取血,分離出血清進行假病毒中和效價檢測。
表18 各型HPV疫苗每隻小鼠免疫劑量(µg)
HPV 型 | 6 | 11 | 16 | 18 | 31 | 33 | 45 | 52 | 58 | 35 | 39 | 51 | 56 | 59 |
劑量 | 0.15 | 0.2 | 0.3 | 0.2 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
假病毒中和法檢測免疫後小鼠血清中和效價
小鼠血清在56度滅活30分鐘後,離心6000g,5分鐘後取上清進行檢測。檢測前4-8小時,將293FT細胞以15000細胞/孔的密度鋪板於96孔板中,培養於37℃,5%CO
2的二氧化碳培養箱中。免疫後小鼠血清、空白對照血清均用中和培養基系列稀釋後按照體積比1:1分別與預稀釋好的14種假病毒(14型HPV假病毒包括6、11、16、18、31、33、35、39、45、51、52、56、58、59型,其製備分別見實施例6.1-6.14)混合。2~8℃冰箱中孵育1小時後按照100μL/孔加入到提前4-8小時鋪板的293FT細胞上,每個樣品2個複孔,同時設立空白血清對照孔、假病毒陽性對照孔和陰性對照孔。加樣後的細胞繼續在37℃,5%CO
2的二氧化碳培養箱中培養62-96小時後,在酶聯斑點分析儀(型號:S6 Universal-V Analyzer,廠家:CTL)中進行螢光掃描拍照以及計數。透過計算每個小鼠血清樣品的中和抑制率,依據Reed-Muench法計算得到血清中和抑制率為50%時血清最大稀釋倍數,即半數有效稀釋倍數EC
50。檢測結果見圖3。
如圖3所示,14價類病毒顆粒免疫組合物能夠產生很好的中和抗體,可以用於製備成預防HPV感染的疫苗。
比較例1:C端截短的HPV16L1(1-474)的表達
發明人嘗試將HPV16L1的C端截短31個胺基酸,命名為HPV16L1(1-474)(SEQ ID NO: 27)。但在研究中發現,截短的HPV16L1(1-474)蛋白表達量高但蛋白可溶性差,難以提取純化,具體表達和提取結果見圖4。
雖然前述已經用說明和實施例的方式對本發明進行了細節描述,但其目的在於理解方便,本發明所屬技術領域中具有通常知識者顯然可以對本發明的技術方案作出的各種變形和改進,而不會偏離附加的申請專利範圍的精神或範圍。
附錄1:序列表-嵌合的人乳頭瘤病毒6型L1蛋白
SEQ ID No: 1 | 0601 HPV 6型L1蛋白的1-469胺基酸序列 MWRPSDSTVYVPPPNPVSKVVATDAYVTRTNIFYHASSSRLLAVGHPYFSIKRANKTVVPKVSGYQYRVFKVVLPDPNKFALPDSSLFDPTTQRLVWACTGLEVGRGQPLGVGVSGHPFLNKYDDVENSGSGGNPGQDNRVNVGMDYKQTQLCMVGCAPPLGEHWGKGKQCTNTPVQAGDCPPLELITSVIQDGDMVDTGFGAMNFADLQTNKSDVPIDICGTTCKYPDYLQMAADPYGDRLFFFLRKEQMFARHFFNRAGEVGEPVPDTLIIKGSGNRTSVGSSIYVNTPSGSLVSSEAQLFNKPYWLQKAQGHNNGICWGNQLFVTVVDTTRSTNMTLCASVTTSSTYTNSDYKEYMRHVEEYDLQFIFQLCSITLSAEVMAYIHTMNPSVLEDWNFGLSPPPNGTLEDTYRYVQSQAITCQKPTPEKEKPDPYKNLSFWEVNLKEKFSSELDQYPLGRKFLLQSGY |
SEQ ID No: 2 | 0602 HPV 33型L1蛋白的474-499胺基酸序列 KAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 3 | 0603 嵌合的HPV 6型L1蛋白的胺基酸序列 MWRPSDSTVYVPPPNPVSKVVATDAYVTRTNIFYHASSSRLLAVGHPYFSIKRANKTVVPKVSGYQYRVFKVVLPDPNKFALPDSSLFDPTTQRLVWACTGLEVGRGQPLGVGVSGHPFLNKYDDVENSGSGGNPGQDNRVNVGMDYKQTQLCMVGCAPPLGEHWGKGKQCTNTPVQAGDCPPLELITSVIQDGDMVDTGFGAMNFADLQTNKSDVPIDICGTTCKYPDYLQMAADPYGDRLFFFLRKEQMFARHFFNRAGEVGEPVPDTLIIKGSGNRTSVGSSIYVNTPSGSLVSSEAQLFNKPYWLQKAQGHNNGICWGNQLFVTVVDTTRSTNMTLCASVTTSSTYTNSDYKEYMRHVEEYDLQFIFQLCSITLSAEVMAYIHTMNPSVLEDWNFGLSPPPNGTLEDTYRYVQSQAITCQKPTPEKEKPDPYKNLSFWEVNLKEKFSSELDQYPLGRKFLLQSGYKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 4 | 0604 嵌合的HPV 6型L1蛋白的核苷酸序列 ATGTGGAGACCATCTGACAGCACAGTCTATGTGCCTCCTCCAAACCCTGTGAGCAAGGTGGTGGCTACAGATGCCTATGTGACCAGGACCAACATCTTCTACCATGCCTCCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAGGGCTAACAAGACAGTGGTGCCAAAGGTGTCTGGCTACCAATACAGGGTGTTCAAGGTGGTGCTGCCTGACCCAAACAAGTTTGCCCTGCCTGACTCCTCCCTGTTTGACCCAACCACCCAGAGACTGGTGTGGGCTTGTACTGGATTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGAGTGTCTGGACACCCATTCCTGAACAAATATGATGATGTGGAGAACTCTGGCTCTGGAGGCAACCCTGGACAAGACAACAGGGTGAATGTGGGGATGGACTACAAGCAGACCCAACTTTGTATGGTGGGCTGTGCCCCTCCACTGGGAGAACACTGGGGCAAGGGCAAGCAGTGTACCAACACACCTGTCCAGGCTGGAGACTGTCCTCCATTGGAACTGATTACCTCTGTGATTCAGGATGGAGATATGGTGGACACAGGCTTTGGAGCTATGAACTTTGCTGACCTCCAAACCAACAAGTCTGATGTGCCAATTGACATCTGTGGCACCACTTGTAAATACCCTGACTACCTCCAAATGGCTGCTGACCCATATGGAGACAGACTGTTCTTCTTCCTGAGGAAGGAACAGATGTTTGCCAGACACTTCTTCAACAGGGCTGGAGAGGTGGGAGAACCTGTGCCTGACACCCTGATTATCAAGGGCTCTGGCAACAGGACCTCTGTGGGCTCCAGCATCTATGTGAACACACCATCTGGCTCCCTGGTGTCCTCTGAGGCTCAACTTTTCAACAAGCCATACTGGCTCCAAAAGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAACTTTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTGCCTCTGTGACCACCTCCAGCACCTACACCAACTCTGACTACAAGGAATATATGAGGCATGTGGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTAGCATCACCCTGTCTGCTGAGGTGATGGCTTACATCCACACAATGAACCCATCTGTGTTGGAGGACTGGAACTTTGGACTGAGCCCTCCTCCAAATGGCACCTTGGAGGACACCTACAGATATGTCCAGAGCCAGGCTATCACTTGTCAGAAGCCAACACCTGAGAAGGAGAAGCCTGACCCATACAAGAACCTGTCCTTCTGGGAGGTGAACCTGAAAGAGAAGTTCTCCTCTGAACTGGACCAATACCCACTGGGCAGGAAGTTCCTGCTCCAATCTGGCTACAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAA |
SEQ ID No: 5 | 0605 合成的HPV6L1基因 ctgggtaccATGTGGAGACCATCTGACAGCACAGTCTATGTGCCTCCTCCAAACCCTGTGAGCAAGGTGGTGGCTACAGATGCCTATGTGACCAGGACCAACATCTTCTACCATGCCTCCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAGGGCTAACAAGACAGTGGTGCCAAAGGTGTCTGGCTACCAATACAGGGTGTTCAAGGTGGTGCTGCCTGACCCAAACAAGTTTGCCCTGCCTGACTCCTCCCTGTTTGACCCAACCACCCAGAGACTGGTGTGGGCTTGTACTGGATTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGAGTGTCTGGACACCCATTCCTGAACAAATATGATGATGTGGAGAACTCTGGCTCTGGAGGCAACCCTGGACAAGACAACAGGGTGAATGTGGGGATGGACTACAAGCAGACCCAACTTTGTATGGTGGGCTGTGCCCCTCCACTGGGAGAACACTGGGGCAAGGGCAAGCAGTGTACCAACACACCTGTCCAGGCTGGAGACTGTCCTCCATTGGAACTGATTACCTCTGTGATTCAGGATGGAGATATGGTGGACACAGGCTTTGGAGCTATGAACTTTGCTGACCTCCAAACCAACAAGTCTGATGTGCCAATTGACATCTGTGGCACCACTTGTAAATACCCTGACTACCTCCAAATGGCTGCTGACCCATATGGAGACAGACTGTTCTTCTTCCTGAGGAAGGAACAGATGTTTGCCAGACACTTCTTCAACAGGGCTGGAGAGGTGGGAGAACCTGTGCCTGACACCCTGATTATCAAGGGCTCTGGCAACAGGACCTCTGTGGGCTCCAGCATCTATGTGAACACACCATCTGGCTCCCTGGTGTCCTCTGAGGCTCAACTTTTCAACAAGCCATACTGGCTCCAAAAGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAACTTTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTGCCTCTGTGACCACCTCCAGCACCTACACCAACTCTGACTACAAGGAATATATGAGGCATGTGGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTAGCATCACCCTGTCTGCTGAGGTGATGGCTTACATCCACACAATGAACCCATCTGTGTTGGAGGACTGGAACTTTGGACTGAGCCCTCCTCCAAATGGCACCTTGGAGGACACCTACAGATATGTCCAGAGCCAGGCTATCACTTGTCAGAAGCCAACACCTGAGAAGGAGAAGCCTGACCCATACAAGAACCTGTCCTTCTGGGAGGTGAACCTGAAAGAGAAGTTCTCCTCTGAACTGGACCAATACCCACTGGGCAGGAAGTTCCTGCTCCAATCTGGCTACAGGGGCAGGTCCAGCATCAGGACAGGAGTGAAGAGACCTGCTGTGAGCAAGGCATCTGCTGCCCCAAAGAGGAAGAGGGCTAAGACCAAGAGGTAAActcgagctc |
SEQ ID No: 6 | 0606 合成的HPV33L1基因 ctgggtaccATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAActcgagctc |
SEQ ID No: 7 | 0607 HPV6L1 F1 CTTGGTACCATGTGGAGACCATCTGACAGCACAGT |
SEQ ID No: 8 | 0608 HPV6L1 R1 GCTTGGCTTTGTAGCCAGATTGGAGCAGGAACTTCC |
SEQ ID No: 9 | 0609 HPV6L1擴增序列1 CTT GGTACC 1 ATGTGGAGACCATCTGACAGCACAGTCTATGTGCCTCCTCCAAACCCTGTGAGCAAGGTG GTGGCTACAGATGCCTATGTGACCAGGACCAACATCTTCTACCATGCCTCCTCCAGCAGA CTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAGGGCTAACAAGACAGTGGTGCCA AAGGTGTCTGGCTACCAATACAGGGTGTTCAAGGTGGTGCTGCCTGACCCAAACAAGTTT GCCCTGCCTGACTCCTCCCTGTTTGACCCAACCACCCAGAGACTGGTGTGGGCTTGTACT GGATTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGAGTGTCTGGACACCCATTCCTG AACAAATATGATGATGTGGAGAACTCTGGCTCTGGAGGCAACCCTGGACAAGACAACAGG GTGAATGTGGGGATGGACTACAAGCAGACCCAACTTTGTATGGTGGGCTGTGCCCCTCCA CTGGGAGAACACTGGGGCAAGGGCAAGCAGTGTACCAACACACCTGTCCAGGCTGGAGAC TGTCCTCCATTGGAACTGATTACCTCTGTGATTCAGGATGGAGATATGGTGGACACAGGC TTTGGAGCTATGAACTTTGCTGACCTCCAAACCAACAAGTCTGATGTGCCAATTGACATC TGTGGCACCACTTGTAAATACCCTGACTACCTCCAAATGGCTGCTGACCCATATGGAGAC AGACTGTTCTTCTTCCTGAGGAAGGAACAGATGTTTGCCAGACACTTCTTCAACAGGGCT GGAGAGGTGGGAGAACCTGTGCCTGACACCCTGATTATCAAGGGCTCTGGCAACAGGACC TCTGTGGGCTCCAGCATCTATGTGAACACACCATCTGGCTCCCTGGTGTCCTCTGAGGCT CAACTTTTCAACAAGCCATACTGGCTCCAAAAGGCTCAAGGACACAACAATGGCATCTGT TGGGGCAACCAACTTTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTG TGTGCCTCTGTGACCACCTCCAGCACCTACACCAACTCTGACTACAAGGAATATATGAGG CATGTGGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTAGCATCACCCTGTCTGCT GAGGTGATGGCTTACATCCACACAATGAACCCATCTGTGTTGGAGGACTGGAACTTTGGA CTGAGCCCTCCTCCAAATGGCACCTTGGAGGACACCTACAGATATGTCCAGAGCCAGGCT ATCACTTGTCAGAAGCCAACACCTGAGAAGGAGAAGCCTGACCCATACAAGAACCTGTCC TTCTGGGAGGTGAACCTGAAAGAGAAGTTCTCCTCTGAACTGGACCAATACCCACTGGGC AGGAAGTTCCTGCTCCAATCTGGCTAC 2 AAAGCCAAGC 31: KpnⅠ酶切位點; 2: 編碼HPV6L1的1-469胺基酸的基因片段; 3: 與HPV33L1的474-499胺基酸的基因片段重疊的10個鹼基。 |
SEQ ID No: 10 | 0610 HPV6L1 F2 ATCTGGCTACAAAGCCAAGCCAAAACTGAAAAGGG |
SEQ ID No: 11 | 0611 HPV6L1 R2 CTGTCTAGATTTACTTCTTCACCTTCTTCCTCTTGGC |
SEQ ID No: 12 | 0612 HPV6L1擴增序列2 ATCTGGCTAC 1 AAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGG ACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAG 2TAA A TCTAGA 3CAG. 1: 與HPV6L1的1-469胺基酸C端基因片段重疊的10bp鹼基; 2: HPV33L1 C端的26個(474-499)胺基酸的基因片段; 3: XbaI酶切位點。 |
SEQ ID No: 13 | 0613 HPV 59型L1蛋白的471-508胺基酸序列 LQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
附錄2:序列表-嵌合的人乳頭瘤病毒11型L1蛋白
SEQ ID No: 14 | 1101 HPV 11型L1蛋白的1-470胺基酸序列 MWRPSDSTVYVPPPNPVSKVVATDAYVKRTNIFYHASSSRLLAVGHPYYSIKKVNKTVVPKVSGYQYRVFKVVLPDPNKFALPDSSLFDPTTQRLVWACTGLEVGRGQPLGVGVSGHPLLNKYDDVENSGGYGGNPGQDNRVNVGMDYKQTQLCMVGCAPPLGEHWGKGTQCSNTSVQNGDCPPLELITSVIQDGDMVDTGFGAMNFADLQTNKSDVPLDICGTVCKYPDYLQMAADPYGDRLFFYLRKEQMFARHFFNRAGTVGEPVPDDLLVKGGNNRSSVASSIYVHTPSGSLVSSEAQLFNKPYWLQKAQGHNNGICWGNHLFVTVVDTTRSTNMTLCASVSKSATYTNSDYKEYMRHVEEFDLQFIFQLCSITLSAEVMAYIHTMNPSVLEDWNFGLSPPPNGTLEDTYRYVQSQAITCQKPTPEKEKQDPYKDMSFWEVNLKEKFSSELDQFPLGRKFLLQSGY |
SEQ ID No: 15 | 1102 HPV 33型L1蛋白的474-499胺基酸序列 KAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 16 | 1103 嵌合的HPV 11型L1蛋白的胺基酸序列 MWRPSDSTVYVPPPNPVSKVVATDAYVKRTNIFYHASSSRLLAVGHPYYSIKKVNKTVVPKVSGYQYRVFKVVLPDPNKFALPDSSLFDPTTQRLVWACTGLEVGRGQPLGVGVSGHPLLNKYDDVENSGGYGGNPGQDNRVNVGMDYKQTQLCMVGCAPPLGEHWGKGTQCSNTSVQNGDCPPLELITSVIQDGDMVDTGFGAMNFADLQTNKSDVPLDICGTVCKYPDYLQMAADPYGDRLFFYLRKEQMFARHFFNRAGTVGEPVPDDLLVKGGNNRSSVASSIYVHTPSGSLVSSEAQLFNKPYWLQKAQGHNNGICWGNHLFVTVVDTTRSTNMTLCASVSKSATYTNSDYKEYMRHVEEFDLQFIFQLCSITLSAEVMAYIHTMNPSVLEDWNFGLSPPPNGTLEDTYRYVQSQAITCQKPTPEKEKQDPYKDMSFWEVNLKEKFSSELDQFPLGRKFLLQSGYKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 17 | 1104 嵌合的HPV 11型L1蛋白的核苷酸序列 ATGTGGAGACCATCTGACAGCACAGTCTATGTGCCTCCTCCAAACCCTGTGAGCAAGGTGGTGGCTACAGATGCCTATGTGAAGAGGACCAACATCTTCTACCATGCCTCCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTACAGCATCAAGAAGGTGAACAAGACAGTGGTGCCAAAGGTGTCTGGCTACCAATACAGGGTGTTCAAGGTGGTGCTGCCTGACCCAAACAAGTTTGCCCTGCCTGACTCCTCCCTGTTTGACCCAACCACCCAGAGACTGGTGTGGGCTTGTACTGGATTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGAGTGTCTGGACACCCACTGCTGAACAAATATGATGATGTGGAGAACTCTGGAGGCTATGGAGGCAACCCTGGACAAGACAACAGGGTGAATGTGGGGATGGACTACAAGCAGACCCAACTTTGTATGGTGGGCTGTGCCCCTCCACTGGGAGAACACTGGGGCAAGGGCACCCAGTGTAGCAACACCTCTGTCCAGAATGGAGACTGTCCTCCATTGGAACTGATTACCTCTGTGATTCAGGATGGAGATATGGTGGACACAGGCTTTGGAGCTATGAACTTTGCTGACCTCCAAACCAACAAGTCTGATGTGCCACTGGACATCTGTGGCACAGTGTGTAAATACCCTGACTACCTCCAAATGGCTGCTGACCCATATGGAGACAGACTGTTCTTCTACCTGAGGAAGGAACAGATGTTTGCCAGACACTTCTTCAACAGGGCTGGCACAGTGGGAGAACCTGTGCCTGATGACCTGCTGGTGAAGGGAGGCAACAACAGGTCCTCTGTGGCATCCAGCATCTATGTGCATACACCATCTGGCTCCCTGGTGTCCTCTGAGGCTCAACTTTTCAACAAGCCATACTGGCTCCAAAAGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCACCTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTGCCTCTGTGAGCAAGTCTGCCACCTACACCAACTCTGACTACAAGGAATATATGAGGCATGTGGAGGAGTTTGACCTCCAATTCATCTTCCAACTTTGTAGCATCACCCTGTCTGCTGAGGTGATGGCTTACATCCACACAATGAACCCATCTGTGTTGGAGGACTGGAACTTTGGACTGAGCCCTCCTCCAAATGGCACCTTGGAGGACACCTACAGATATGTCCAGAGCCAGGCTATCACTTGTCAGAAGCCAACACCTGAGAAGGAGAAGCAGGACCCATACAAGGATATGAGTTTCTGGGAGGTGAACCTGAAAGAGAAGTTCTCCTCTGAACTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAATCTGGCTACAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAA |
SEQ ID No: 18 | 1105 合成的HPV11L1基因 ctgggtaccATGTGGAGACCATCTGACAGCACAGTCTATGTGCCTCCTCCAAACCCTGTGAGCAAGGTGGTGGCTACAGATGCCTATGTGAAGAGGACCAACATCTTCTACCATGCCTCCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTACAGCATCAAGAAGGTGAACAAGACAGTGGTGCCAAAGGTGTCTGGCTACCAATACAGGGTGTTCAAGGTGGTGCTGCCTGACCCAAACAAGTTTGCCCTGCCTGACTCCTCCCTGTTTGACCCAACCACCCAGAGACTGGTGTGGGCTTGTACTGGATTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGAGTGTCTGGACACCCACTGCTGAACAAATATGATGATGTGGAGAACTCTGGAGGCTATGGAGGCAACCCTGGACAAGACAACAGGGTGAATGTGGGGATGGACTACAAGCAGACCCAACTTTGTATGGTGGGCTGTGCCCCTCCACTGGGAGAACACTGGGGCAAGGGCACCCAGTGTAGCAACACCTCTGTCCAGAATGGAGACTGTCCTCCATTGGAACTGATTACCTCTGTGATTCAGGATGGAGATATGGTGGACACAGGCTTTGGAGCTATGAACTTTGCTGACCTCCAAACCAACAAGTCTGATGTGCCACTGGACATCTGTGGCACAGTGTGTAAATACCCTGACTACCTCCAAATGGCTGCTGACCCATATGGAGACAGACTGTTCTTCTACCTGAGGAAGGAACAGATGTTTGCCAGACACTTCTTCAACAGGGCTGGCACAGTGGGAGAACCTGTGCCTGATGACCTGCTGGTGAAGGGAGGCAACAACAGGTCCTCTGTGGCATCCAGCATCTATGTGCATACACCATCTGGCTCCCTGGTGTCCTCTGAGGCTCAACTTTTCAACAAGCCATACTGGCTCCAAAAGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCACCTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTGCCTCTGTGAGCAAGTCTGCCACCTACACCAACTCTGACTACAAGGAATATATGAGGCATGTGGAGGAGTTTGACCTCCAATTCATCTTCCAACTTTGTAGCATCACCCTGTCTGCTGAGGTGATGGCTTACATCCACACAATGAACCCATCTGTGTTGGAGGACTGGAACTTTGGACTGAGCCCTCCTCCAAATGGCACCTTGGAGGACACCTACAGATATGTCCAGAGCCAGGCTATCACTTGTCAGAAGCCAACACCTGAGAAGGAGAAGCAGGACCCATACAAGGATATGAGTTTCTGGGAGGTGAACCTGAAAGAGAAGTTCTCCTCTGAACTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAATCTGGCTACAGGGGCAGGACCTCTGCCAGGACAGGCATCAAGAGACCTGCTGTGAGCAAGCCAAGCACAGCCCCAAAGAGGAAGAGGACCAAGACCAAGAAGTAAActcgagctc |
SEQ ID No: 19 | 1106 合成的HPV33L1基因 ctgggtaccATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAActcgagctc |
SEQ ID No: 20 | 1107 HPV11L1 F1 CTTGGTACCATGTGGAGACCATCTGACAGCACAGT |
SEQ ID No: 21 | 1108 HPV11L1 R1 GCTTGGCTTTGTAGCCAGATTGGAGCAGGAACTTCC |
SEQ ID No: 22 | 1109 HPV11L1擴增序列1 CTT GGTACC 1 ATGTGGAGACCATCTGACAGCACAGTCTATGTGCCTCCTCCAAACCCTGTGAGCAAGGTGGTGGCTACAGATGCCTATGTGAAGAGGACCAACATCTTCTACCATGCCTCCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTACAGCATCAAGAAGGTGAACAAGACAGTGGTGCCAAAGGTGTCTGGCTACCAATACAGGGTGTTCAAGGTGGTGCTGCCTGACCCAAACAAGTTTGCCCTGCCTGACTCCTCCCTGTTTGACCCAACCACCCAGAGACTGGTGTGGGCTTGTACTGGATTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGAGTGTCTGGACACCCACTGCTGAACAAATATGATGATGTGGAGAACTCTGGAGGCTATGGAGGCAACCCTGGACAAGACAACAGGGTGAATGTGGGGATGGACTACAAGCAGACCCAACTTTGTATGGTGGGCTGTGCCCCTCCACTGGGAGAACACTGGGGCAAGGGCACCCAGTGTAGCAACACCTCTGTCCAGAATGGAGACTGTCCTCCATTGGAACTGATTACCTCTGTGATTCAGGATGGAGATATGGTGGACACAGGCTTTGGAGCTATGAACTTTGCTGACCTCCAAACCAACAAGTCTGATGTGCCACTGGACATCTGTGGCACAGTGTGTAAATACCCTGACTACCTCCAAATGGCTGCTGACCCATATGGAGACAGACTGTTCTTCTACCTGAGGAAGGAACAGATGTTTGCCAGACACTTCTTCAACAGGGCTGGCACAGTGGGAGAACCTGTGCCTGATGACCTGCTGGTGAAGGGAGGCAACAACAGGTCCTCTGTGGCATCCAGCATCTATGTGCATACACCATCTGGCTCCCTGGTGTCCTCTGAGGCTCAACTTTTCAACAAGCCATACTGGCTCCAAAAGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCACCTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTGCCTCTGTGAGCAAGTCTGCCACCTACACCAACTCTGACTACAAGGAATATATGAGGCATGTGGAGGAGTTTGACCTCCAATTCATCTTCCAACTTTGTAGCATCACCCTGTCTGCTGAGGTGATGGCTTACATCCACACAATGAACCCATCTGTGTTGGAGGACTGGAACTTTGGACTGAGCCCTCCTCCAAATGGCACCTTGGAGGACACCTACAGATATGTCCAGAGCCAGGCTATCACTTGTCAGAAGCCAACACCTGAGAAGGAGAAGCAGGACCCATACAAGGATATGAGTTTCTGGGAGGTGAACCTGAAAGAGAAGTTCTCCTCTGAACTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAATCTGGCTAC 2 AAAGCCAAGC 31: KpnⅠ酶切位點; 2: 編碼HPV11L1的1-470胺基酸的基因片段; 3: 與HPV33L1的474-499胺基酸的基因片段重疊的10個鹼基。 |
SEQ ID No: 23 | 1110 HPV11L1 F2 ATCTGGCTACAAAGCCAAGCCAAAACTGAAAAGGG |
SEQ ID No: 24 | 1111 HPV11L1 R2 CTGTCTAGATTTACTTCTTCACCTTCTTCCTCTTGGC |
SEQ ID No: 25 | 1112 HPV11L1擴增序列2 ATCTGGCTAC 1 AAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGG ACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAG 2TAA A TCTAGA 3CAG. 1: 與HPV11L1的1-470胺基酸C端基因片段重疊的10bp鹼基; 2: HPV33L1 C端的26個(474-499)胺基酸的基因片段; 3: XbaI酶切位點。 |
SEQ ID No: 26 | 1113 HPV 59型L1蛋白的471-508胺基酸序列 LQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
附錄3:序列表-嵌合的人乳頭瘤病毒16型L1蛋白
SEQ ID No: 27 | 1601 HPV 16型L1蛋白的1-474胺基酸序列 MSLWLPSEATVYLPPVPVSKVVSTDEYVARTNIYYHAGTSRLLAVGHPYFPIKKPNNNKILVPKVSGLQYRVFRIHLPDPNKFGFPDTSFYNPDTQRLVWACVGVEVGRGQPLGVGISGHPLLNKLDDTENASAYAANAGVDNRECISMDYKQTQLCLIGCKPPIGEHWGKGSPCTNVAVNPGDCPPLELINTVIQDGDMVDTGFGAMDFTTLQANKSEVPLDICTSICKYPDYIKMVSEPYGDSLFFYLRREQMFVRHLFNRAGAVGENVPDDLYIKGSGSTANLASSNYFPTPSGSMVTSDAQIFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMSLCAAISTSETTYKNTNFKEYLRHGEEYDLQFIFQLCKITLTADVMTYIHSMNSTILEDWNFGLQPPPGGTLEDTYRFVTSQAIACQKHTPPAPKEDPLKKYTFWEVNLKEKFSADLDQFPLGRKFLLQAGL |
SEQ ID No: 28 | 1602 HPV 33型L1蛋白的474-499胺基酸序列 KAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 29 | 1603 嵌合的HPV 16型L1蛋白的胺基酸序列 MSLWLPSEATVYLPPVPVSKVVSTDEYVARTNIYYHAGTSRLLAVGHPYFPIKKPNNNKILVPKVSGLQYRVFRIHLPDPNKFGFPDTSFYNPDTQRLVWACVGVEVGRGQPLGVGISGHPLLNKLDDTENASAYAANAGVDNRECISMDYKQTQLCLIGCKPPIGEHWGKGSPCTNVAVNPGDCPPLELINTVIQDGDMVDTGFGAMDFTTLQANKSEVPLDICTSICKYPDYIKMVSEPYGDSLFFYLRREQMFVRHLFNRAGAVGENVPDDLYIKGSGSTANLASSNYFPTPSGSMVTSDAQIFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMSLCAAISTSETTYKNTNFKEYLRHGEEYDLQFIFQLCKITLTADVMTYIHSMNSTILEDWNFGLQPPPGGTLEDTYRFVTSQAIACQKHTPPAPKEDPLKKYTFWEVNLKEKFSADLDQFPLGRKFLLQAGLKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 30 | 1604 嵌合的HPV 16型L1蛋白的核苷酸序列 ATGAGTCTGTGGCTGCCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGGCAAGGACCAACATCTACTACCATGCTGGCACCAGCAGACTGCTGGCTGTGGGACACCCATACTTTCCAATCAAGAAGCCAAACAACAACAAGATTCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGATTCACCTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGAGTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAACTGGATGACACAGAGAATGCCTCTGCCTATGCTGCCAATGCTGGAGTGGACAACAGGGAGTGTATCAGTATGGACTACAAGCAGACCCAACTTTGTCTGATTGGCTGTAAGCCTCCAATTGGAGAACACTGGGGCAAGGGCAGCCCATGTACCAATGTGGCTGTGAACCCTGGAGACTGTCCTCCATTGGAACTGATAAACACAGTGATTCAGGATGGAGATATGGTGGACACAGGCTTTGGAGCTATGGACTTCACCACCCTCCAAGCCAACAAGTCTGAGGTGCCACTGGACATCTGTACCAGCATCTGTAAATACCCTGACTACATCAAGATGGTGTCTGAACCATATGGAGACTCCCTGTTCTTCTACCTGAGGAGGGAACAGATGTTTGTGAGACACCTGTTCAACAGGGCTGGAGCAGTGGGAGAGAATGTGCCTGATGACCTCTACATCAAGGGCTCTGGCAGCACAGCCAACCTGGCATCCAGCAACTACTTTCCAACACCATCTGGCAGTATGGTGACCTCTGATGCCCAGATTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAACTTTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGAGTCTGTGTGCTGCCATCAGCACCTCTGAGACCACCTACAAGAACACCAACTTCAAGGAATACCTGAGACATGGAGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTAAGATTACCCTGACAGCAGATGTGATGACCTACATCCACAGTATGAACAGCACCATCTTGGAGGACTGGAACTTTGGACTCCAACCTCCTCCTGGAGGCACCTTGGAGGACACCTACAGGTTTGTGACCAGCCAGGCTATTGCCTGTCAGAAACACACACCTCCTGCCCCAAAGGAGGACCCACTGAAAAAATACACCTTCTGGGAGGTGAACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAA |
SEQ ID No: 31 | 1605 合成的HPV16L1基因 ctgggtaccATGAGTCTGTGGCTGCCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGGCAAGGACCAACATCTACTACCATGCTGGCACCAGCAGACTGCTGGCTGTGGGACACCCATACTTTCCAATCAAGAAGCCAAACAACAACAAGATTCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGATTCACCTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGAGTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAACTGGATGACACAGAGAATGCCTCTGCCTATGCTGCCAATGCTGGAGTGGACAACAGGGAGTGTATCAGTATGGACTACAAGCAGACCCAACTTTGTCTGATTGGCTGTAAGCCTCCAATTGGAGAACACTGGGGCAAGGGCAGCCCATGTACCAATGTGGCTGTGAACCCTGGAGACTGTCCTCCATTGGAACTGATAAACACAGTGATTCAGGATGGAGATATGGTGGACACAGGCTTTGGAGCTATGGACTTCACCACCCTCCAAGCCAACAAGTCTGAGGTGCCACTGGACATCTGTACCAGCATCTGTAAATACCCTGACTACATCAAGATGGTGTCTGAACCATATGGAGACTCCCTGTTCTTCTACCTGAGGAGGGAACAGATGTTTGTGAGACACCTGTTCAACAGGGCTGGAGCAGTGGGAGAGAATGTGCCTGATGACCTCTACATCAAGGGCTCTGGCAGCACAGCCAACCTGGCATCCAGCAACTACTTTCCAACACCATCTGGCAGTATGGTGACCTCTGATGCCCAGATTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAACTTTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGAGTCTGTGTGCTGCCATCAGCACCTCTGAGACCACCTACAAGAACACCAACTTCAAGGAATACCTGAGACATGGAGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTAAGATTACCCTGACAGCAGATGTGATGACCTACATCCACAGTATGAACAGCACCATCTTGGAGGACTGGAACTTTGGACTCCAACCTCCTCCTGGAGGCACCTTGGAGGACACCTACAGGTTTGTGACCAGCCAGGCTATTGCCTGTCAGAAACACACACCTCCTGCCCCAAAGGAGGACCCACTGAAAAAATACACCTTCTGGGAGGTGAACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAGTTCACCCTGGGCAAGAGGAAGGCTACACCAACCACCTCCAGCACCAGCACCACAGCCAAGAGGAAGAAGAGGAAACTGTAAActcgagctc |
SEQ ID No: 32 | 1606 合成的HPV33L1基因 ctgggtaccATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAActcgagctc |
SEQ ID No: 33 | 1607 HPV16L1 F1 CTTGGTACCATGAGTCTGTGGCTGCCATCTGAGG |
SEQ ID No: 34 | 1608 HPV16L1 R1 GCTTGGCTTTCAGTCCTGCTTGGAGCAGGAACTTCC |
SEQ ID No: 35 | 1609 HPV16L1擴增序列1 CTT GGTACC 1 ATGAGTCTGTGGCTGCCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGGCAAGGACCAACATCTACTACCATGCTGGCACCAGCAGACTGCTGGCTGTGGGACACCCATACTTTCCAATCAAGAAGCCAAACAACAACAAGATTCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGATTCACCTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGAGTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAACTGGATGACACAGAGAATGCCTCTGCCTATGCTGCCAATGCTGGAGTGGACAACAGGGAGTGTATCAGTATGGACTACAAGCAGACCCAACTTTGTCTGATTGGCTGTAAGCCTCCAATTGGAGAACACTGGGGCAAGGGCAGCCCATGTACCAATGTGGCTGTGAACCCTGGAGACTGTCCTCCATTGGAACTGATAAACACAGTGATTCAGGATGGAGATATGGTGGACACAGGCTTTGGAGCTATGGACTTCACCACCCTCCAAGCCAACAAGTCTGAGGTGCCACTGGACATCTGTACCAGCATCTGTAAATACCCTGACTACATCAAGATGGTGTCTGAACCATATGGAGACTCCCTGTTCTTCTACCTGAGGAGGGAACAGATGTTTGTGAGACACCTGTTCAACAGGGCTGGAGCAGTGGGAGAGAATGTGCCTGATGACCTCTACATCAAGGGCTCTGGCAGCACAGCCAACCTGGCATCCAGCAACTACTTTCCAACACCATCTGGCAGTATGGTGACCTCTGATGCCCAGATTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAACTTTTTGTGACAGTGG TGGACACCACCAGGAGCACCAATATGAGTCTGTGTGCTGCCATCAGCACCTCTGAGACCACCTACAAGAACACCAACTTCAAGGAATACCTGAGACATGGAGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTAAGATTACCCTGACAGCAGATGTGATGACCTACATCCACAGTATGAACAGCACCATCTTGGAGGACTGGAACTTTGGACTCCAACCTCCTCCTGGAGGCACCTTGGAGGACACCTACAGGTTTGTGACCAGCCAGGCTATTGCCTGTCAGAAACACACACCTCCTGCCCCAAAGGAGGACCCACTGAAAAAATACACCTTCTGGGAGGTGAACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTG 2 AAAGCCAAGC 31: KpnⅠ酶切位點; 2: 編碼HPV16L1的1-474胺基酸的基因片段; 3: 與HPV33L1的474-499胺基酸的基因片段重疊的10個鹼基。 |
SEQ ID No: 36 | 1610 HPV16L1 F2 AGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGG |
SEQ ID No: 37 | 1611 HPV16L1 R2 CTGTCTAGATTTACTTCTTCACCTTCTTCCTCTTGG |
SEQ ID No: 38 | 1612 HPV16L1擴增序列2 AGCAGGACTG 1 AAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGG ACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAG 2TAA A TCTAGA 3CAG. 1: 與HPV16L1的1-474胺基酸C端基因片段重疊的10bp鹼基; 2: HPV33L1 C端的26個(474-499)胺基酸的基因片段; 3: XbaI酶切位點。 |
SEQ ID No: 39 | 1613 HPV 59型L1蛋白的471-508胺基酸序列 LQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
附錄4:序列表-嵌合的人乳頭瘤病毒18型L1蛋白
SEQ ID No: 40 | 1801 HPV 18型L1蛋白的1-470胺基酸序列 MALWRPSDNTVYLPPPSVARVVNTDDYVTRTSIFYHAGSSRLLTVGNPYFRVPAGGGNKQDIPKVSAYQYRVFRVQLPDPNKFGLPDTSIYNPETQRLVWACAGVEIGRGQPLGVGLSGHPFYNKLDDTESSHAATSNVSEDVRDNVSVDYKQTQLCILGCAPAIGEHWAKGTACKSRPLSQGDCPPLELKNTVLEDGDMVDTGYGAMDFSTLQDTKCEVPLDICQSICKYPDYLQMSADPYGDSMFFCLRREQLFARHFWNRAGTMGDTVPQSLYIKGTGMRASPGSCVYSPSPSGSIVTSDSQLFNKPYWLHKAQGHNNGVCWHNQLFVTVVDTTRSTNLTICASTQSPVPGQYDATKFKQYSRHVEEYDLQFIFQLCTITLTADVMSYIHSMNSSILEDWNFGVPPPPTTSLVDTYRFVQSVAITCQKDAAPAENKDPYDKLKFWNVDLKEKFSLDLDQYPLGRKFL |
SEQ ID No: 41 | 1802 HPV 33型L1蛋白的474-499胺基酸序列 KAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 42 | 1803 嵌合的HPV 18型L1蛋白的胺基酸序列 MALWRPSDNTVYLPPPSVARVVNTDDYVTRTSIFYHAGSSRLLTVGNPYFRVPAGGGNKQDIPKVSAYQYRVFRVQLPDPNKFGLPDTSIYNPETQRLVWACAGVEIGRGQPLGVGLSGHPFYNKLDDTESSHAATSNVSEDVRDNVSVDYKQTQLCILGCAPAIGEHWAKGTACKSRPLSQGDCPPLELKNTVLEDGDMVDTGYGAMDFSTLQDTKCEVPLDICQSICKYPDYLQMSADPYGDSMFFCLRREQLFARHFWNRAGTMGDTVPQSLYIKGTGMRASPGSCVYSPSPSGSIVTSDSQLFNKPYWLHKAQGHNNGVCWHNQLFVTVVDTTRSTNLTICASTQSPVPGQYDATKFKQYSRHVEEYDLQFIFQLCTITLTADVMSYIHSMNSSILEDWNFGVPPPPTTSLVDTYRFVQSVAITCQKDAAPAENKDPYDKLKFWNVDLKEKFSLDLDQYPLGRKFLKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 43 | 1804 嵌合的HPV 18型L1蛋白的核苷酸序列 ATGGCCCTCTGGAGACCATCCGATAACACAGTGTACTTGCCCCCACCCAGCGTCGCCCGGGTGGTGAACACAGACGACTACGTCACCAGAACCTCAATCTTCTACCACGCCGGGTCCAGCCGGCTGCTGACCGTGGGCAACCCCTACTTCCGCGTGCCCGCCGGCGGCGGAAACAAACAAGACATCCCCAAAGTCAGCGCCTATCAGTACCGGGTGTTCCGCGTCCAACTGCCCGATCCCAACAAGTTCGGCCTGCCCGACACCTCCATCTACAACCCCGAGACCCAGAGGCTGGTCTGGGCTTGCGCCGGCGTCGAGATCGGGAGGGGCCAACCCCTGGGCGTGGGGTTGTCCGGCCACCCCTTCTACAACAAGCTGGACGATACCGAGTCCAGCCACGCAGCAACCAGCAACGTCTCCGAAGATGTGCGCGATAACGTCAGCGTGGACTACAAACAAACCCAACTGTGCATCCTGGGATGCGCACCCGCCATCGGCGAGCATTGGGCCAAGGGGACCGCCTGCAAGAGCAGGCCCCTGAGCCAAGGGGACTGTCCACCCCTGGAGTTGAAGAATACCGTGCTCGAGGACGGCGACATGGTGGACACCGGCTACGGCGCTATGGATTTCTCCACCCTCCAGGACACCAAGTGCGAAGTGCCCCTCGACATCTGCCAAAGCATCTGCAAGTACCCCGACTACCTCCAGATGAGCGCCGACCCCTACGGCGACAGCATGTTCTTCTGTCTCAGAAGGGAACAATTGTTCGCCCGCCACTTCTGGAACCGGGCCGGCACAATGGGAGATACAGTCCCCCAGAGCCTGTACATCAAGGGGACCGGAATGAGGGCCAGCCCCGGGTCCTGCGTCTACAGCCCAAGCCCCTCCGGGAGCATCGTCACAAGCGATAGCCAACTCTTCAACAAGCCCTACTGGCTCCACAAAGCCCAAGGCCACAATAACGGGGTGTGTTGGCACAACCAGCTGTTCGTGACCGTCGTGGACACAACCAGGTCCACAAACCTGACCATCTGCGCCAGCACCCAAAGCCCCGTGCCCGGCCAGTACGACGCCACAAAGTTCAAACAATACTCTCGGCACGTGGAAGAGTACGACCTCCAATTCATCTTCCAACTCTGCACCATCACCCTCACCGCCGACGTGATGAGCTACATCCACTCCATGAACTCCTCCATCCTGGAAGACTGGAATTTCGGCGTGCCACCACCCCCTACCACCTCCCTCGTCGACACCTACAGATTCGTGCAGAGCGTGGCCATCACATGCCAGAAAGACGCCGCCCCCGCCGAGAACAAAGACCCATACGACAAACTGAAATTCTGGAACGTCGACCTGAAAGAGAAATTCAGCCTGGATCTGGACCAGTACCCATTGGGCAGGAAGTTCCTCAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAA |
SEQ ID No: 44 | 1805 合成的HPV18L1基因 ctgggtaccATGGCCCTCTGGAGACCATCCGATAACACAGTGTACTTGCCCCCACCCAGCGTCGCCCGGGTGGTGAACACAGACGACTACGTCACCAGAACCTCAATCTTCTACCACGCCGGGTCCAGCCGGCTGCTGACCGTGGGCAACCCCTACTTCCGCGTGCCCGCCGGCGGCGGAAACAAACAAGACATCCCCAAAGTCAGCGCCTATCAGTACCGGGTGTTCCGCGTCCAACTGCCCGATCCCAACAAGTTCGGCCTGCCCGACACCTCCATCTACAACCCCGAGACCCAGAGGCTGGTCTGGGCTTGCGCCGGCGTCGAGATCGGGAGGGGCCAACCCCTGGGCGTGGGGTTGTCCGGCCACCCCTTCTACAACAAGCTGGACGATACCGAGTCCAGCCACGCAGCAACCAGCAACGTCTCCGAAGATGTGCGCGATAACGTCAGCGTGGACTACAAACAAACCCAACTGTGCATCCTGGGATGCGCACCCGCCATCGGCGAGCATTGGGCCAAGGGGACCGCCTGCAAGAGCAGGCCCCTGAGCCAAGGGGACTGTCCACCCCTGGAGTTGAAGAATACCGTGCTCGAGGACGGCGACATGGTGGACACCGGCTACGGCGCTATGGATTTCTCCACCCTCCAGGACACCAAGTGCGAAGTGCCCCTCGACATCTGCCAAAGCATCTGCAAGTACCCCGACTACCTCCAGATGAGCGCCGACCCCTACGGCGACAGCATGTTCTTCTGTCTCAGAAGGGAACAATTGTTCGCCCGCCACTTCTGGAACCGGGCCGGCACAATGGGAGATACAGTCCCCCAGAGCCTGTACATCAAGGGGACCGGAATGAGGGCCAGCCCCGGGTCCTGCGTCTACAGCCCAAGCCCCTCCGGGAGCATCGTCACAAGCGATAGCCAACTCTTCAACAAGCCCTACTGGCTCCACAAAGCCCAAGGCCACAATAACGGGGTGTGTTGGCACAACCAGCTGTTCGTGACCGTCGTGGACACAACCAGGTCCACAAACCTGACCATCTGCGCCAGCACCCAAAGCCCCGTGCCCGGCCAGTACGACGCCACAAAGTTCAAACAATACTCTCGGCACGTGGAAGAGTACGACCTCCAATTCATCTTCCAACTCTGCACCATCACCCTCACCGCCGACGTGATGAGCTACATCCACTCCATGAACTCCTCCATCCTGGAAGACTGGAATTTCGGCGTGCCACCACCCCCTACCACCTCCCTCGTCGACACCTACAGATTCGTGCAGAGCGTGGCCATCACATGCCAGAAAGACGCCGCCCCCGCCGAGAACAAAGACCCATACGACAAACTGAAATTCTGGAACGTCGACCTGAAAGAGAAATTCAGCCTGGATCTGGACCAGTACCCATTGGGCAGGAAGTTCCTCGTGCAAGCCGGCCTCAGGAGAAAACCAACAATCGGGCCCAGGAAGAGGAGCGCCCCCAGCGCAACCACCAGCAGCAAGCCCGCAAAAAGGGTCAGAGTGAGGGCACGCAAATAAActcgagctc |
SEQ ID No: 45 | 1806 合成的HPV33L1基因 ctgggtaccATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAA ctcgagctc |
SEQ ID No: 46 | 1807 HPV18L1 F1 CTTGGTACCATGGCCCTCTGGAGACCATCCGATA |
SEQ ID No: 47 | 1808 HPV18L1 R1 GCTTGGCTTTGAGGAACTTCCTGCCCAATGGGTAC |
SEQ ID No: 48 | 1809 HPV18L1擴增序列1 CTT GGTACC 1 ATGGCCCTCTGGAGACCATCCGATAACACAGTGTACTTGCCCCCACCCAGCGTCGCCCGGGTGGTGAACACAGACGACTACGTCACCAGAACCTCAATCTTCTACCACGCCGGGTCCAGCCGGCTGCTGACCGTGGGCAACCCCTACTTCCGCGTGCCCGCCGGCGGCGGAAACAAACAAGACATCCCCAAAGTCAGCGCCTATCAGTACCGGGTGTTCCGCGTCCAACTGCCCGATCCCAACAAGTTCGGCCTGCCCGACACCTCCATCTACAACCCCGAGACCCAGAGGCTGGTCTGGGCTTGCGCCGGCGTCGAGATCGGGAGGGGCCAACCCCTGGGCGTGGGGTTGTCCGGCCACCCCTTCTACAACAAGCTGGACGATACCGAGTCCAGCCACGCAGCAACCAGCAACGTCTCCGAAGATGTGCGCGATAACGTCAGCGTGGACTACAAACAAACCCAACTGTGCATCCTGGGATGCGCACCCGCCATCGGCGAGCATTGGGCCAAGGGGACCGCCTGCAAGAGCAGGCCCCTGAGCCAAGGGGACTGTCCACCCCTGGAGTTGAAGAATACCGTGCTCGAGGACGGCGACATGGTGGACACCGGCTACGGCGCTATGGATTTCTCCACCCTCCAGGACACCAAGTGCGAAGTGCCCCTCGACATCTGCCAAAGCATCTGCAAGTACCCCGACTACCTCCAGATGAGCGCCGACCCCTACGGCGACAGCATGTTCTTCTGTCTCAGAAGGGAACAATTGTTCGCCCGCCACTTCTGGAACCGGGCCGGCACAATGGGAGATACAGTCCCCCAGAGCCTGTACATCAAGGGGACCGGAATGAGGGCCAGCCCCGGGTCCTGCGTCTACAGCCCAAGCCCCTCCGGGAGCATCGTCACAAGCGATAGCCAACTCTTCAACAAGCCCTACTGGCTCCACAAAGCCCAAGGCCACAATAACGGGGTGTGTTGGCACAACCAGCTGTTCGTGACCGTCG TGGACACAACCAGGTCCACAAACCTGACCATCTGCGCCAGCACCCAAAGCCCCGTGCCCGGCCAGTACGACGCCACAAAGTTCAAACAATACTCTCGGCACGTGGAAGAGTACGACCTCCAATTCATCTTCCAACTCTGCACCATCACCCTCACCGCCGACGTGATGAGCTACATCCACTCCATGAACTCCTCCATCCTGGAAGACTGGAATTTCGGCGTGCCACCACCCCCTACCACCTCCCTCGTCGACACCTACAGATTCGTGCAGAGCGTGGCCATCACATGCCAGAAAGACGCCGCCCCCGCCGAGAACAAAGACCCATACGACAAACTGAAATTCTGGAACGTCGACCTGAAAGAGAAATTCAGCCTGGATCTGGACCAGTACCCATTGGGCAGGAAGTTCCTC 2 AAAGCCAAGC 31: KpnⅠ酶切位點; 2: 編碼HPV18L1的1-470胺基酸的基因片段; 3: 與HPV33L1的474-499胺基酸的基因片段重疊的10個鹼基。 |
SEQ ID No: 49 | 1810 HPV18L1 F2 GAAGTTCCTCAAAGCCAAGCCAAAACTGAAAAGGG |
SEQ ID No: 50 | 1811 HPV18L1 R2 CTGTCTAGATTTACTTCTTCACCTTCTTCCTCTTGG |
SEQ ID No: 51 | 1812 HPV18L1擴增序列2 GAAGTTCCTC 1 AAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGG ACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAG 2TAA A TCTAGA 3CAG .1: 與HPV18L1的1-470胺基酸C端基因片段重疊的10bp鹼基; 2: HPV33L1 C端的26個(474-499)胺基酸的基因片段; 3: XbaI酶切位點。 |
SEQ ID No: 52 | 1813 HPV 59型L1蛋白的471-508胺基酸序列 LQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
附錄5:序列表-嵌合的人乳頭瘤病毒31型L1蛋白
SEQ ID No: 53 | 3101 HPV 31型L1蛋白的1-475胺基酸序列 MSLWRPSEATVYLPPVPVSKVVSTDEYVTRTNIYYHAGSARLLTVGHPYYSIPKSDNPKKIVVPKVSGLQYRVFRVRLPDPNKFGFPDTSFYNPETQRLVWACVGLEVGRGQPLGVGISGHPLLNKFDDTENSNRYAGGPGTDNRECISMDYKQTQLCLLGCKPPIGEHWGKGSPCSNNAITPGDCPPLELKNSVIQDGDMVDTGFGAMDFTALQDTKSNVPLDICNSICKYPDYLKMVAEPYGDTLFFYLRREQMFVRHFFNRSGTVGESVPTDLYIKGSGSTATLANSTYFPTPSGSMVTSDAQIFNKPYWMQRAQGHNNGICWGNQLFVTVVDTTRSTNMSVCAAIANSDTTFKSSNFKEYLRHGEEFDLQFIFQLCKITLSADIMTYIHSMNPAILEDWNFGLTTPPSGSLEDTYRFVTSQAITCQKSAPQKPKEDPFKDYVFWEVNLKEKFSADLDQFPLGRKFLLQAGY |
SEQ ID No: 54 | 3102 HPV 33型L1蛋白的474-499胺基酸序列 KAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 55 | 3103 嵌合的HPV 31型L1蛋白的胺基酸序列 MSLWRPSEATVYLPPVPVSKVVSTDEYVTRTNIYYHAGSARLLTVGHPYYSIPKSDNPKKIVVPKVSGLQYRVFRVRLPDPNKFGFPDTSFYNPETQRLVWACVGLEVGRGQPLGVGISGHPLLNKFDDTENSNRYAGGPGTDNRECISMDYKQTQLCLLGCKPPIGEHWGKGSPCSNNAITPGDCPPLELKNSVIQDGDMVDTGFGAMDFTALQDTKSNVPLDICNSICKYPDYLKMVAEPYGDTLFFYLRREQMFVRHFFNRSGTVGESVPTDLYIKGSGSTATLANSTYFPTPSGSMVTSDAQIFNKPYWMQRAQGHNNGICWGNQLFVTVVDTTRSTNMSVCAAIANSDTTFKSSNFKEYLRHGEEFDLQFIFQLCKITLSADIMTYIHSMNPAILEDWNFGLTTPPSGSLEDTYRFVTSQAITCQKSAPQKPKEDPFKDYVFWEVNLKEKFSADLDQFPLGRKFLLQAGYKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 56 | 3104 嵌合的HPV 31型L1蛋白的核苷酸序列 ATGAGCCTGTGGAGGCCCAGCGAGGCCACCGTGTACCTGCCCCCCGTGCCCGTGAGCAAGGTGGTGAGCACCGACGAGTACGTGACCAGGACCAACATCTACTACCACGCCGGCAGCGCCAGGCTGCTGACCGTGGGCCACCCCTACTACAGCATCCCCAAGAGCGACAACCCCAAGAAGATCGTGGTGCCCAAGGTGAGCGGCCTGCAGTACAGGGTGTTCAGGGTGAGGCTGCCCGACCCCAACAAGTTCGGCTTCCCCGACACCAGCTTCTACAACCCCGAGACCCAGAGGCTGGTGTGGGCCTGCGTGGGCCTGGAGGTGGGCAGGGGCCAGCCCCTGGGCGTGGGCATCAGCGGCCACCCCCTGCTGAACAAGTTCGACGACACCGAGAACAGCAACAGGTACGCCGGCGGCCCCGGCACCGACAACAGGGAGTGCATCAGCATGGACTACAAGCAGACCCAGCTGTGCCTGCTGGGCTGCAAGCCCCCCATCGGCGAGCACTGGGGCAAGGGCAGCCCCTGCAGCAACAACGCCATCACCCCCGGCGACTGCCCCCCCCTGGAGCTGAAGAACAGCGTGATCCAGGACGGCGACATGGTGGACACCGGCTTCGGCGCCATGGACTTCACCGCCCTGCAGGACACCAAGAGCAACGTGCCCCTGGACATCTGCAACAGCATCTGCAAGTACCCCGACTACCTGAAGATGGTGGCCGAGCCCTACGGCGACACCCTGTTCTTCTACCTGAGGAGGGAGCAGATGTTCGTGAGGCACTTCTTCAACAGGAGCGGCACCGTGGGCGAGAGCGTGCCCACCGACCTGTACATCAAGGGCAGCGGCAGCACCGCCACCCTGGCCAACAGCACCTACTTCCCCACCCCCAGCGGCAGCATGGTGACCAGCGACGCCCAGATCTTCAACAAGCCCTACTGGATGCAGAGGGCCCAGGGCCACAACAACGGCATCTGCTGGGGCAACCAGCTGTTCGTGACCGTGGTGGACACCACCAGGAGCACCAACATGAGCGTGTGCGCCGCCATCGCCAACAGCGACACCACCTTCAAGAGCAGCAACTTCAAGGAGTACCTGAGGCACGGCGAGGAGTTCGACCTGCAGTTCATCTTCCAGCTGTGCAAGATCACCCTGAGCGCCGACATCATGACCTACATCCACAGCATGAACCCCGCCATCCTGGAGGACTGGAACTTCGGCCTGACCACCCCCCCCAGCGGCAGCCTGGAGGACACCTACAGGTTCGTGACCAGCCAGGCCATCACCTGCCAGAAGTCCGCCCCCCAGAAGCCCAAGGAGGACCCCTTCAAGGACTACGTGTTCTGGGAGGTGAACCTGAAGGAGAAGTTCAGCGCCGACCTGGACCAGTTCCCCCTGGGCAGGAAGTTCCTGCTGCAGGCCGGCTACAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAA |
SEQ ID No: 57 | 3105 合成的HPV31L1基因 ctgggtaccATGAGCCTGTGGAGGCCCAGCGAGGCCACCGTGTACCTGCCCCCCGTGCCCGTGAGCAAGGTGGTGAGCACCGACGAGTACGTGACCAGGACCAACATCTACTACCACGCCGGCAGCGCCAGGCTGCTGACCGTGGGCCACCCCTACTACAGCATCCCCAAGAGCGACAACCCCAAGAAGATCGTGGTGCCCAAGGTGAGCGGCCTGCAGTACAGGGTGTTCAGGGTGAGGCTGCCCGACCCCAACAAGTTCGGCTTCCCCGACACCAGCTTCTACAACCCCGAGACCCAGAGGCTGGTGTGGGCCTGCGTGGGCCTGGAGGTGGGCAGGGGCCAGCCCCTGGGCGTGGGCATCAGCGGCCACCCCCTGCTGAACAAGTTCGACGACACCGAGAACAGCAACAGGTACGCCGGCGGCCCCGGCACCGACAACAGGGAGTGCATCAGCATGGACTACAAGCAGACCCAGCTGTGCCTGCTGGGCTGCAAGCCCCCCATCGGCGAGCACTGGGGCAAGGGCAGCCCCTGCAGCAACAACGCCATCACCCCCGGCGACTGCCCCCCCCTGGAGCTGAAGAACAGCGTGATCCAGGACGGCGACATGGTGGACACCGGCTTCGGCGCCATGGACTTCACCGCCCTGCAGGACACCAAGAGCAACGTGCCCCTGGACATCTGCAACAGCATCTGCAAGTACCCCGACTACCTGAAGATGGTGGCCGAGCCCTACGGCGACACCCTGTTCTTCTACCTGAGGAGGGAGCAGATGTTCGTGAGGCACTTCTTCAACAGGAGCGGCACCGTGGGCGAGAGCGTGCCCACCGACCTGTACATCAAGGGCAGCGGCAGCACCGCCACCCTGGCCAACAGCACCTACTTCCCCACCCCCAGCGGCAGCATGGTGACCAGCGACGCCCAGATCTTCAACAAGCCCTACTGGATGCAGAGGGCCCAGGGCCACAACAACGGCATCTGCTGGGGCAACCAGCTGTTCGTGACCGTGGTGGACACCACCAGGAGCACCAACATGAGCGTGTGCGCCGCCATCGCCAACAGCGACACCACCTTCAAGAGCAGCAACTTCAAGGAGTACCTGAGGCACGGCGAGGAGTTCGACCTGCAGTTCATCTTCCAGCTGTGCAAGATCACCCTGAGCGCCGACATCATGACCTACATCCACAGCATGAACCCCGCCATCCTGGAGGACTGGAACTTCGGCCTGACCACCCCCCCCAGCGGCAGCCTGGAGGACACCTACAGGTTCGTGACCAGCCAGGCCATCACCTGCCAGAAGTCCGCCCCCCAGAAGCCCAAGGAGGACCCCTTCAAGGACTACGTGTTCTGGGAGGTGAACCTGAAGGAGAAGTTCAGCGCCGACCTGGACCAGTTCCCCCTGGGCAGGAAGTTCCTGCTGCAGGCCGGCTACAGGGCCAGGCCCAAGTTCAAGGCCGGCAAGAGGAGCGCCCCCAGCGCCAGCACCACCACCCCCGCCAAGAGGAAGAAGACCAAGAAGTAAActcgagctc |
SEQ ID No: 58 | 3106 合成的HPV33L1基因 ctgggtaccATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAActcgagctc |
SEQ ID No: 59 | 3107 HPV31L1 F1 CTTGGTACCATGAGCCTGTGGAGGCCCAGCGAG |
SEQ ID No: 60 | 3108 HPV31L1 R1 GCTTGGCTTTGTAGCCGGCCTGCAGCAGGAACTTCCTG |
SEQ ID No: 61 | 3109 HPV31L1擴增序列1 CTT GGTACC 1 ATGAGCCTGTGGAGGCCCAGCGAGGCCACCGTGTACCTGCCCCCCGTGCCCGTGAGCAAGGTGGTGAGCACCGACGAGTACGTGACCAGGACCAACATCTACTACCACGCCGGCAGCGCCAGGCTGCTGACCGTGGGCCACCCCTACTACAGCATCCCCAAGAGCGACAACCCCAAGAAGATCGTGGTGCCCAAGGTGAGCGGCCTGCAGTACAGGGTGTTCAGGGTGAGGCTGCCCGACCCCAACAAGTTCGGCTTCCCCGACACCAGCTTCTACAACCCCGAGACCCAGAGGCTGGTGTGGGCCTGCGTGGGCCTGGAGGTGGGCAGGGGCCAGCCCCTGGGCGTGGGCATCAGCGGCCACCCCCTGCTGAACAAGTTCGACGACACCGAGAACAGCAACAGGTACGCCGGCGGCCCCGGCACCGACAACAGGGAGTGCATCAGCATGGACTACAAGCAGACCCAGCTGTGCCTGCTGGGCTGCAAGCCCCCCATCGGCGAGCACTGGGGCAAGGGCAGCCCCTGCAGCAACAACGCCATCACCCCCGGCGACTGCCCCCCCCTGGAGCTGAAGAACAGCGTGATCCAGGACGGCGACATGGTGGACACCGGCTTCGGCGCCATGGACTTCACCGCCCTGCAGGACACCAAGAGCAACGTGCCCCTGGACATCTGCAACAGCATCTGCAAGTACCCCGACTACCTGAAGATGGTGGCCGAGCCCTACGGCGACACCCTGTTCTTCTACCTGAGGAGGGAGCAGATGTTCGTGAGGCACTTCTTCAACAGGAGCGGCACCGTGGGCGAGAGCGTGCCCACCGACCTGTACATCAAGGGCAGCGGCAGCACCGCCACCCTGGCCAACAGCACCTACTTCCCCACCCCCAGCGGCAGCATGGTGACCAGCGACGCCCAGATCTTCAACAAGCCCTACTGGATGCAGAGGGCCCAGGGCCACAACAACGGCATCTGCTGGGGCAACCAGCTGTTCGTGACCG TGGTGGACACCACCAGGAGCACCAACATGAGCGTGTGCGCCGCCATCGCCAACAGCGACACCACCTTCAAGAGCAGCAACTTCAAGGAGTACCTGAGGCACGGCGAGGAGTTCGACCTGCAGTTCATCTTCCAGCTGTGCAAGATCACCCTGAGCGCCGACATCATGACCTACATCCACAGCATGAACCCCGCCATCCTGGAGGACTGGAACTTCGGCCTGACCACCCCCCCCAGCGGCAGCCTGGAGGACACCTACAGGTTCGTGACCAGCCAGGCCATCACCTGCCAGAAGTCCGCCCCCCAGAAGCCCAAGGAGGACCCCTTCAAGGACTACGTGTTCTGGGAGGTGAACCTGAAGGAGAAGTTCAGCGCCGACCTGGACCAGTTCCCCCTGGGCAGGAAGTTCCTGCTGCAGGCCGGCTAC 2 AAAGCCAAGC 31: KpnⅠ酶切位點; 2: 編碼HPV31L1的1-475胺基酸的基因片段; 3: 與HPV33L1的474-499胺基酸的基因片段重疊的10個鹼基。 |
SEQ ID No: 62 | 3110 HPV31L1 F2 GGCCGGCTACAAAGCCAAGCCAAAACTGAAAAGGG |
SEQ ID No: 63 | 3111 HPV31L1 R2 CTGTCTAGATTTACTTCTTCACCTTCTTCCTCTTGGCAGAG |
SEQ ID No: 64 | 3112 HPV31L1擴增序列2 GGCCGGCTAC 1 AAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGG ACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAG 2TAA A TCTAGA 3CAG. 1: 與HPV31L1的1-475胺基酸C端基因片段重疊的10bp鹼基; 2: HPV33L1 C端的26個(474-499)胺基酸的基因片段; 3: XbaI酶切位點。 |
SEQ ID No: 65 | 3113 HPV 59型L1蛋白的471-508胺基酸序列 LQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
附錄6:序列表-人乳頭瘤病毒33型L1蛋白
SEQ ID No: 66 | 3301 HPV 33型L1蛋白的胺基酸序列 MSVWRPSEATVYLPPVPVSKVVSTDEYVSRTSIYYYAGSSRLLAVGHPYFSIKNPTNAKKLLVPKVSGLQYRVFRVRLPDPNKFGFPDTSFYNPDTQRLVWACVGLEIGRGQPLGVGISGHPLLNKFDDTETGNKYPGQPGADNRECLSMDYKQTQLCLLGCKPPTGEHWGKGVACTNAAPANDCPPLELINTIIEDGDMVDTGFGCMDFKTLQANKSDVPIDICGSTCKYPDYLKMTSEPYGDSLFFFLRREQMFVRHFFNRAGTLGEAVPDDLYIKGSGTTASIQSSAFFPTPSGSMVTSESQLFNKPYWLQRAQGHNNGICWGNQVFVTVVDTTRSTNMTLCTQVTSDSTYKNENFKEYIRHVEEYDLQFVFQLCKVTLTAEVMTYIHAMNPDILEDWQFGLTPPPSASLQDTYRFVTSQAITCQKTVPPKEKEDPLGKYTFWEVDLKEKFSADLDQFPLGRKFLLQAGLKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 67 | 3302 HPV 33型L1蛋白的核苷酸序列 ATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAA |
SEQ ID No: 68 | 3303 合成的HPV33L1基因 ctgggtaccATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAActcgagctc |
附錄7:序列表-嵌合的人乳頭瘤病毒35型L1蛋白
SEQ ID No: 69 | 3501 HPV 35型L1蛋白的1-472胺基酸序列 MSLWRSNEATVYLPPVSVSKVVSTDEYVTRTNIYYHAGSSRLLAVGHPYYAIKKQDSNKIAVPKVSGLQYRVFRVKLPDPNKFGFPDTSFYDPASQRLVWACTGVEVGRGQPLGVGISGHPLLNKLDDTENSNKYVGNSGTDNRECISMDYKQTQLCLIGCRPPIGEHWGKGTPCNANQVKAGECPPLELLNTVLQDGDMVDTGFGAMDFTTLQANKSDVPLDICSSICKYPDYLKMVSEPYGDMLFFYLRREQMFVRHLFNRAGTVGETVPADLYIKGTTGTLPSTSYFPTPSGSMVTSDAQIFNKPYWLQRAQGHNNGICWSNQLFVTVVDTTRSTNMSVCSAVSSSDSTYKNDNFKEYLRHGEEYDLQFIFQLCKITLTADVMTYIHSMNPSILEDWNFGLTPPPSGTLEDTYRYVTSQAVTCQKPSAPKPKDDPLKNYTFWEVDLKEKFSADLDQFPLGRKFLLQAGL |
SEQ ID No: 70 | 3502 HPV 33型L1蛋白的474-499胺基酸序列 KAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 71 | 3503 嵌合的HPV 35型L1蛋白的胺基酸序列 MSLWRSNEATVYLPPVSVSKVVSTDEYVTRTNIYYHAGSSRLLAVGHPYYAIKKQDSNKIAVPKVSGLQYRVFRVKLPDPNKFGFPDTSFYDPASQRLVWACTGVEVGRGQPLGVGISGHPLLNKLDDTENSNKYVGNSGTDNRECISMDYKQTQLCLIGCRPPIGEHWGKGTPCNANQVKAGECPPLELLNTVLQDGDMVDTGFGAMDFTTLQANKSDVPLDICSSICKYPDYLKMVSEPYGDMLFFYLRREQMFVRHLFNRAGTVGETVPADLYIKGTTGTLPSTSYFPTPSGSMVTSDAQIFNKPYWLQRAQGHNNGICWSNQLFVTVVDTTRSTNMSVCSAVSSSDSTYKNDNFKEYLRHGEEYDLQFIFQLCKITLTADVMTYIHSMNPSILEDWNFGLTPPPSGTLEDTYRYVTSQAVTCQKPSAPKPKDDPLKNYTFWEVDLKEKFSADLDQFPLGRKFLLQAGLKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 72 | 3504 嵌合的HPV 35型L1蛋白的核苷酸序列 ATGAGTCTGTGGAGGAGCAATGAGGCTACAGTCTACCTGCCTCCTGTGTCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGACCAGGACCAACATCTACTACCATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTATGCCATCAAGAAGCAGGACAGCAACAAGATTGCTGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAAACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTATGACCCTGCCAGCCAGAGACTGGTGTGGGCTTGTACTGGAGTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAACTGGATGACACAGAGAACAGCAACAAATATGTGGGCAACTCTGGCACAGACAACAGGGAGTGTATCAGTATGGACTACAAGCAGACCCAACTTTGTCTGATTGGCTGTAGACCTCCAATTGGAGAACACTGGGGCAAGGGCACACCATGTAATGCCAACCAGGTGAAGGCTGGAGAGTGTCCTCCATTGGAACTGCTGAACACAGTGCTCCAAGATGGAGATATGGTGGACACAGGCTTTGGAGCTATGGACTTCACCACCCTCCAAGCCAACAAGTCTGATGTGCCACTGGACATCTGTTCCAGCATCTGTAAATACCCTGACTACCTGAAAATGGTGTCTGAACCATATGGAGATATGCTGTTCTTCTACCTGAGGAGGGAACAGATGTTTGTGAGACACCTGTTCAACAGGGCTGGCACAGTGGGAGAGACAGTGCCTGCTGACCTCTACATCAAGGGCACCACAGGCACCCTGCCAAGCACCTCCTACTTTCCAACACCATCTGGCAGTATGGTGACCTCTGATGCCCAGATTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGAGCAACCAACTTTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGAGTGTGTGTTCTGCTGTGTCCTCCTCTGACAGCACCTACAAGAATGACAACTTCAAGGAATACCTGAGACATGGAGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTAAGATTACCCTGACAGCAGATGTGATGACCTACATCCACAGTATGAACCCAAGCATCTTGGAGGACTGGAACTTTGGACTGACACCTCCTCCATCTGGCACCTTGGAGGACACCTACAGATATGTGACCAGCCAGGCTGTGACTTGTCAGAAGCCATCTGCCCCAAAGCCAAAGGATGACCCACTGAAAAACTACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAA |
SEQ ID No: 73 | 3505 合成的HPV35L1基因 ctgggtaccATGAGTCTGTGGAGGAGCAATGAGGCTACAGTCTACCTGCCTCCTGTGTCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGACCAGGACCAACATCTACTACCATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTATGCCATCAAGAAGCAGGACAGCAACAAGATTGCTGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAAACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTATGACCCTGCCAGCCAGAGACTGGTGTGGGCTTGTACTGGAGTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAACTGGATGACACAGAGAACAGCAACAAATATGTGGGCAACTCTGGCACAGACAACAGGGAGTGTATCAGTATGGACTACAAGCAGACCCAACTTTGTCTGATTGGCTGTAGACCTCCAATTGGAGAACACTGGGGCAAGGGCACACCATGTAATGCCAACCAGGTGAAGGCTGGAGAGTGTCCTCCATTGGAACTGCTGAACACAGTGCTCCAAGATGGAGATATGGTGGACACAGGCTTTGGAGCTATGGACTTCACCACCCTCCAAGCCAACAAGTCTGATGTGCCACTGGACATCTGTTCCAGCATCTGTAAATACCCTGACTACCTGAAAATGGTGTCTGAACCATATGGAGATATGCTGTTCTTCTACCTGAGGAGGGAACAGATGTTTGTGAGACACCTGTTCAACAGGGCTGGCACAGTGGGAGAGACAGTGCCTGCTGACCTCTACATCAAGGGCACCACAGGCACCCTGCCAAGCACCTCCTACTTTCCAACACCATCTGGCAGTATGGTGACCTCTGATGCCCAGATTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGAGCAACCAACTTTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGAGTGTGTGTTCTGCTGTGTCCTCCTCTGACAGCACCTACAAGAATGACAACTTCAAGGAATACCTGAGACATGGAGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTAAGATTACCCTGACAGCAGATGTGATGACCTACATCCACAGTATGAACCCAAGCATCTTGGAGGACTGGAACTTTGGACTGACACCTCCTCCATCTGGCACCTTGGAGGACACCTACAGATATGTGACCAGCCAGGCTGTGACTTGTCAGAAGCCATCTGCCCCAAAGCCAAAGGATGACCCACTGAAAAACTACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAGACCAAACTTCAGACTGGGCAAGAGGGCTGCCCCTGCCAGCACCAGCAAGAAGTCCAGCACCAAGAGGAGGAAGGTGAAGAGCTAAActcgagctc |
SEQ ID No: 74 | 3506 合成的HPV33L1基因 ctgggtaccATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAActcgagctc |
SEQ ID No: 75 | 3507 HPV35L1 F1 CTTGGTACCATGAGTCTGTGGAGGAGCAATGAGG |
SEQ ID No: 76 | 3508 HPV35L1 R1 GCTTGGCTTTCAGTCCTGCTTGGAGCAGGAACTTCC |
SEQ ID No: 77 | 3509 HPV35L1擴增序列1 CTT GGTACC 1 ATGAGTCTGTGGAGGAGCAATGAGGCTACAGTCTACCTGCCTCCTGTGTCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGACCAGGACCAACATCTACTACCATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTATGCCATCAAGAAGCAGGACAGCAACAAGATTGCTGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAAACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTATGACCCTGCCAGCCAGAGACTGGTGTGGGCTTGTACTGGAGTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAACTGGATGACACAGAGAACAGCAACAAATATGTGGGCAACTCTGGCACAGACAACAGGGAGTGTATCAGTATGGACTACAAGCAGACCCAACTTTGTCTGATTGGCTGTAGACCTCCAATTGGAGAACACTGGGGCAAGGGCACACCATGTAATGCCAACCAGGTGAAGGCTGGAGAGTGTCCTCCATTGGAACTGCTGAACACAGTGCTCCAAGATGGAGATATGGTGGACACAGGCTTTGGAGCTATGGACTTCACCACCCTCCAAGCCAACAAGTCTGATGTGCCACTGGACATCTGTTCCAGCATCTGTAAATACCCTGACTACCTGAAAATGGTGTCTGAACCATATGGAGATATGCTGTTCTTCTACCTGAGGAGGGAACAGATGTTTGTGAGACACCTGTTCAACAGGGCTGGCACAGTGGGAGAGACAGTGCCTGCTGACCTCTACATCAAGGGCACCACAGGCACCCTGCCAAGCACCTCCTACTTTCCAACACCATCTGGCAGTATGGTGACCTCTGATGCCCAGATTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGAGCAACCAACTTTTTGTGACAGTGGTGGACA CCACCAGGAGCACCAATATGAGTGTGTGTTCTGCTGTGTCCTCCTCTGACAGCACCTACAAGAATGACAACTTCAAGGAATACCTGAGACATGGAGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTAAGATTACCCTGACAGCAGATGTGATGACCTACATCCACAGTATGAACCCAAGCATCTTGGAGGACTGGAACTTTGGACTGACACCTCCTCCATCTGGCACCTTGGAGGACACCTACAGATATGTGACCAGCCAGGCTGTGACTTGTCAGAAGCCATCTGCCCCAAAGCCAAAGGATGACCCACTGAAAAACTACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTG 2 AAAGCCAAGC 31: KpnⅠ酶切位點; 2: 編碼HPV35L1的1-472胺基酸的基因片段; 3: 與HPV33L1的474-499胺基酸的基因片段重疊的10個鹼基。 |
SEQ ID No: 78 | 3510 HPV35L1 F2 AGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGG |
SEQ ID No: 79 | 3511 HPV35L1 R2 CTGTCTAGATTTACTTCTTCACCTTCTTCCTCTTGG |
SEQ ID No: 80 | 3512 HPV35L1擴增序列2 AGCAGGACTG 1 AAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGG ACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAG 2TAA A TCTAGA 3CAG. 1: 與HPV35L1的1-472胺基酸C端基因片段重疊的10bp鹼基; 2: HPV33L1 C端的26個(474-499)胺基酸的基因片段; 3: XbaI酶切位點。 |
SEQ ID No: 81 | 3513 HPV 59型L1蛋白的471-508胺基酸序列 LQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
附錄8:序列表-嵌合的人乳頭瘤病毒39型L1蛋白
SEQ ID No: 82 | 3901 HPV 39型L1蛋白的1-469胺基酸序列 MAMWRSSDSMVYLPPPSVAKVVNTDDYVTRTGIYYYAGSSRLLTVGHPYFKVGMNGGRKQDIPKVSAYQYRVFRVTLPDPNKFSIPDASLYNPETQRLVWACVGVEVGRGQPLGVGISGHPLYNRQDDTENSPFSSTTNKDSRDNVSVDYKQTQLCIIGCVPAIGEHWGKGKACKPNNVSTGDCPPLELVNTPIEDGDMIDTGYGAMDFGALQETKSEVPLDICQSICKYPDYLQMSADVYGDSMFFCLRREQLFARHFWNRGGMVGDAIPAQLYIKGTDIRANPGSSVYCPSPSGSMVTSDSQLFNKPYWLHKAQGHNNGICWHNQLFLTVVDTTRSTNFTLSTSIESSIPSTYDPSKFKEYTRHVEEYDLQFIFQLCTVTLTTDVMSYIHTMNSSILDNWNFAVAPPPSASLVDTYRYLQSAAITCQKDAPAPEKKDPYDGLKFWNVDLREKFSLELDQFPLGRKFL |
SEQ ID No: 83 | 3902 HPV 59型L1蛋白的471-508胺基酸序列 LQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
SEQ ID No: 84 | 3903 嵌合的HPV 39型L1蛋白的胺基酸序列 MAMWRSSDSMVYLPPPSVAKVVNTDDYVTRTGIYYYAGSSRLLTVGHPYFKVGMNGGRKQDIPKVSAYQYRVFRVTLPDPNKFSIPDASLYNPETQRLVWACVGVEVGRGQPLGVGISGHPLYNRQDDTENSPFSSTTNKDSRDNVSVDYKQTQLCIIGCVPAIGEHWGKGKACKPNNVSTGDCPPLELVNTPIEDGDMIDTGYGAMDFGALQETKSEVPLDICQSICKYPDYLQMSADVYGDSMFFCLRREQLFARHFWNRGGMVGDAIPAQLYIKGTDIRANPGSSVYCPSPSGSMVTSDSQLFNKPYWLHKAQGHNNGICWHNQLFLTVVDTTRSTNFTLSTSIESSIPSTYDPSKFKEYTRHVEEYDLQFIFQLCTVTLTTDVMSYIHTMNSSILDNWNFAVAPPPSASLVDTYRYLQSAAITCQKDAPAPEKKDPYDGLKFWNVDLREKFSLELDQFPLGRKFLLQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
SEQ ID No: 85 | 3904 嵌合的HPV 39型L1蛋白的核苷酸序列 ATGGCTATGTGGAGGTCCTCTGACAGTATGGTCTACCTGCCTCCTCCATCTGTGGCTAAGGTGGTGAACACAGATGACTATGTGACCAGGACAGGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGACAGTGGGACACCCATACTTCAAGGTGGGGATGAATGGAGGCAGGAAGCAGGACATCCCAAAGGTGTCTGCCTACCAATACAGGGTGTTCAGGGTGACCCTGCCTGACCCAAACAAGTTCAGCATCCCTGATGCCTCCCTCTACAACCCTGAGACCCAGAGACTGGTGTGGGCTTGTGTGGGAGTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTCTACAACAGACAGGATGACACAGAGAACAGCCCATTCTCCAGCACCACCAACAAGGACAGCAGGGACAATGTGTCTGTGGACTACAAGCAGACCCAACTTTGTATCATTGGCTGTGTGCCTGCCATTGGAGAACACTGGGGCAAGGGCAAGGCTTGTAAGCCAAACAATGTGAGCACAGGAGACTGTCCTCCATTGGAACTGGTGAACACACCAATTGAGGATGGAGATATGATTGACACAGGCTATGGAGCTATGGACTTTGGAGCCCTCCAAGAGACCAAGTCTGAGGTGCCACTGGACATCTGTCAGAGCATCTGTAAATACCCTGACTACCTCCAAATGAGTGCTGATGTCTATGGAGACAGTATGTTCTTCTGTCTGAGGAGGGAACAACTTTTTGCCAGACACTTCTGGAACAGGGGAGGGATGGTGGGAGATGCCATCCCTGCCCAACTCTACATCAAGGGCACAGACATCAGGGCTAACCCTGGCTCCTCTGTCTACTGTCCAAGCCCATCTGGCAGTATGGTGACCTCTGACAGCCAACTTTTCAACAAGCCATACTGGCTGCACAAGGCTCAAGGACACAACAATGGCATCTGTTGGCACAACCAACTTTTCCTGACAGTGGTGGACACCACCAGGAGCACCAACTTCACCCTGAGCACCAGCATTGAGTCCAGCATCCCAAGCACCTATGACCCAAGCAAGTTCAAGGAATACACCAGGCATGTGGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTACTGTGACCCTGACCACAGATGTGATGAGTTACATCCACACAATGAACTCCAGCATCCTGGACAACTGGAACTTTGCTGTGGCTCCTCCTCCATCTGCCTCCCTGGTGGACACCTACAGATACCTCCAATCTGCTGCCATCACTTGTCAGAAGGATGCCCCTGCCCCTGAGAAGAAGGACCCATATGATGGACTGAAGTTCTGGAATGTGGACCTGAGGGAGAAGTTCTCCTTGGAACTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAACTTGGAGCCAGACCAAAGCCAACCATTGGACCAAGGAAGAGGGCTGCCCCTGCCCCAACCAGCACACCAAGCCCAAAGAGGGTGAAGAGGAGGAAGTCCAGCAGGAAGTAAA |
SEQ ID No: 86 | 3905 合成的HPV39L1基因 ctgggtaccATGGCTATGTGGAGGTCCTCTGACAGTATGGTCTACCTGCCTCCTCCATCTGTGGCTAAGGTGGTGAACACAGATGACTATGTGACCAGGACAGGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGACAGTGGGACACCCATACTTCAAGGTGGGGATGAATGGAGGCAGGAAGCAGGACATCCCAAAGGTGTCTGCCTACCAATACAGGGTGTTCAGGGTGACCCTGCCTGACCCAAACAAGTTCAGCATCCCTGATGCCTCCCTCTACAACCCTGAGACCCAGAGACTGGTGTGGGCTTGTGTGGGAGTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTCTACAACAGACAGGATGACACAGAGAACAGCCCATTCTCCAGCACCACCAACAAGGACAGCAGGGACAATGTGTCTGTGGACTACAAGCAGACCCAACTTTGTATCATTGGCTGTGTGCCTGCCATTGGAGAACACTGGGGCAAGGGCAAGGCTTGTAAGCCAAACAATGTGAGCACAGGAGACTGTCCTCCATTGGAACTGGTGAACACACCAATTGAGGATGGAGATATGATTGACACAGGCTATGGAGCTATGGACTTTGGAGCCCTCCAAGAGACCAAGTCTGAGGTGCCACTGGACATCTGTCAGAGCATCTGTAAATACCCTGACTACCTCCAAATGAGTGCTGATGTCTATGGAGACAGTATGTTCTTCTGTCTGAGGAGGGAACAACTTTTTGCCAGACACTTCTGGAACAGGGGAGGGATGGTGGGAGATGCCATCCCTGCCCAACTCTACATCAAGGGCACAGACATCAGGGCTAACCCTGGCTCCTCTGTCTACTGTCCAAGCCCATCTGGCAGTATGGTGACCTCTGACAGCCAACTTTTCAACAAGCCATACTGGCTGCACAAGGCTCAAGGACACAACAATGGCATCTGTTGGCACAACCAACTTTTCCTGACAGTGGTGGACACCACCAGGAGCACCAACTTCACCCTGAGCACCAGCATTGAGTCCAGCATCCCAAGCACCTATGACCCAAGCAAGTTCAAGGAATACACCAGGCATGTGGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTACTGTGACCCTGACCACAGATGTGATGAGTTACATCCACACAATGAACTCCAGCATCCTGGACAACTGGAACTTTGCTGTGGCTCCTCCTCCATCTGCCTCCCTGGTGGACACCTACAGATACCTCCAATCTGCTGCCATCACTTGTCAGAAGGATGCCCCTGCCCCTGAGAAGAAGGACCCATATGATGGACTGAAGTTCTGGAATGTGGACCTGAGGGAGAAGTTCTCCTTGGAACTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCCAGGGTGAGGAGGAGACCAACCATTGGACCAAGGAAGAGACCTGCTGCCAGCACCTCCTCCTCCTCTGCCACCAAACACAAGAGGAAGAGGGTGAGCAAGTAAActcgagctc |
SEQ ID No: 87 | 3906 合成的HPV59L1基因 ctgggtaccATGGCTCTGTGGAGGTCCTCTGACAACAAGGTCTACCTGCCTCCTCCATCTGTGGCTAAGGTGGTGAGCACAGATGAATATGTGACCAGGACCAGCATCTTCTACCATGCTGGCTCCAGCAGACTGCTGACAGTGGGACACCCATACTTCAAGGTGCCAAAGGGAGGCAATGGCAGACAGGATGTGCCAAAGGTGTCTGCCTACCAATACAGGGTGTTCAGGGTGAAACTGCCTGACCCAAACAAGTTTGGACTGCCTGACAACACAGTCTATGACCCAAACAGCCAGAGACTGGTGTGGGCTTGTGTGGGAGTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGACTGTCTGGACACCCACTCTACAACAAACTGGATGACACAGAGAACTCTCATGTGGCATCTGCTGTGGACACCAAGGACACCAGGGACAATGTGTCTGTGGACTACAAGCAGACCCAACTTTGTATCATTGGCTGTGTGCCTGCCATTGGAGAACACTGGACCAAGGGCACAGCCTGTAAGCCAACCACAGTGGTCCAGGGAGACTGTCCTCCATTGGAACTGATAAACACACCAATTGAGGATGGAGATATGGTGGACACAGGCTATGGAGCTATGGACTTCAAACTGCTCCAAGACAACAAGTCTGAGGTGCCACTGGACATCTGTCAGAGCATCTGTAAATACCCTGACTACCTCCAAATGAGTGCTGATGCCTATGGAGACAGTATGTTCTTCTGTCTGAGGAGGGAACAGGTGTTTGCCAGACACTTCTGGAACAGGTCTGGCACAATGGGAGACCAACTTCCTGAGTCCCTCTACATCAAGGGCACAGACATCAGGGCTAACCCTGGCTCCTACCTCTACAGCCCAAGCCCATCTGGCTCTGTGGTGACCTCTGACAGCCAACTTTTCAACAAGCCATACTGGCTGCACAAGGCTCAAGGACTGAACAATGGCATCTGTTGGCACAACCAACTTTTCCTGACAGTGGTGGACACCACCAGGAGCACCAACCTGTCTGTGTGTGCCAGCACCACCTCCAGCATCCCAAATGTCTACACACCAACCTCCTTCAAGGAATATGCCAGGCATGTGGAGGAGTTTGACCTCCAATTCATCTTCCAACTTTGTAAGATTACCCTGACCACAGAGGTGATGAGTTACATCCACAATATGAACACCACCATCTTGGAGGACTGGAACTTTGGAGTGACACCTCCTCCAACAGCCTCCCTGGTGGACACCTACAGGTTTGTCCAGTCTGCTGCTGTGACTTGTCAGAAGGACACAGCCCCTCCTGTGAAGCAGGACCCATATGACAAACTGAAGTTCTGGCCTGTGGACCTGAAAGAGAGGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAACTTGGAGCCAGACCAAAGCCAACCATTGGACCAAGGAAGAGGGCTGCCCCTGCCCCAACCAGCACACCAAGCCCAAAGAGGGTGAAGAGGAGGAAGTCCAGCAGGAAGTAAA ctcgagctc |
SEQ ID No: 88 | 3907 HPV39L1 F1 CTTGGTACCATGGCTATGTGGAGGTCCTCTGACAGTAT |
SEQ ID No: 89 | 3908 HPV39L1 R1 TCCAAGTTGGAGCAGGAACTTCCTGCCCAGTGGAAACT |
SEQ ID No: 90 | 3909 HPV39L1擴增序列1 CTT GGTACC 1 ATGGCTATGTGGAGGTCCTCTGACAGTATGGTCTACCTGCCTCCTCCATCTGTGGCTAAGGTGGTGAACACAGATGACTATGTGACCAGGACAGGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGACAGTGGGACACCCATACTTCAAGGTGGGGATGAATGGAGGCAGGAAGCAGGACATCCCAAAGGTGTCTGCCTACCAATACAGGGTGTTCAGGGTGACCCTGCCTGACCCAAACAAGTTCAGCATCCCTGATGCCTCCCTCTACAACCCTGAGACCCAGAGACTGGTGTGGGCTTGTGTGGGAGTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTCTACAACAGACAGGATGACACAGAGAACAGCCCATTCTCCAGCACCACCAACAAGGACAGCAGGGACAATGTGTCTGTGGACTACAAGCAGACCCAACTTTGTATCATTGGCTGTGTGCCTGCCATTGGAGAACACTGGGGCAAGGGCAAGGCTTGTAAGCCAAACAATGTGAGCACAGGAGACTGTCCTCCATTGGAACTGGTGAACACACCAATTGAGGATGGAGATATGATTGACACAGGCTATGGAGCTATGGACTTTGGAGCCCTCCAAGAGACCAAGTCTGAGGTGCCACTGGACATCTGTCAGAGCATCTGTAAATACCCTGACTACCTCCAAATGAGTGCTGATGTCTATGGAGACAGTATGTTCTTCTGTCTGAGGAGGGAACAACTTTTTGCCAGACACTTCTGGAACAGGGGAGGGATGGTGGGAGATGCCATCCCTGCCCAACTCTACATCAAGGGCACAGACATCAGGGCTAACCCTGGCTCCTCTGTCTACTGTCCAAGCCCATCTGGCAGTATGGTGACCTCTGACAGCCAACTTTTCAACAAGCCATACTGGCTGCACAAGGCTCAAGGACACAACAATGGCATCTGTTGGCACAACCAACTTTTCCTGACAGTGGTGG ACACCACCAGGAGCACCAACTTCACCCTGAGCACCAGCATTGAGTCCAGCATCCCAAGCACCTATGACCCAAGCAAGTTCAAGGAATACACCAGGCATGTGGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTACTGTGACCCTGACCACAGATGTGATGAGTTACATCCACACAATGAACTCCAGCATCCTGGACAACTGGAACTTTGCTGTGGCTCCTCCTCCATCTGCCTCCCTGGTGGACACCTACAGATACCTCCAATCTGCTGCCATCACTTGTCAGAAGGATGCCCCTGCCCCTGAGAAGAAGGACCCATATGATGGACTGAAGTTCTGGAATGTGGACCTGAGGGAGAAGTTCTCCTTGGAACTGGACCAGTTTCCACTGGGCAGGAAGTTCCTG 2 CTCCAACTTGGA 31: KpnⅠ酶切位點; 2: 編碼HPV39L1的1-469胺基酸的基因片段; 3: 與HPV59L1的471-508胺基酸的基因片段重疊的12個鹼基。 |
SEQ ID No: 91 | 3910 HPV39L1 F2 AGGAAGTTCCTGCTCCAACTTGGAGCCAGACCAAAGC |
SEQ ID No: 92 | 3911 HPV39L1 R2 CTGTCTAGATTTACTTCCTGCTGGACTTCCTCCTCTTCAC |
SEQ ID No: 93 | 3912 HPV39L1擴增序列2 AGGAAGTTCCTG 1 CTCCAACTTGGAGCCAGACCAAAGCCAACCATTGGACCAAGGAAGAGGGCTGCCCCTGCCCCAACCAGCACACCAAGCCCAAAGAGGGTGAAGAGGAGGAAGTCCAGCAGGAAG 2TAA A TCTAGA 3CAG. 1: 與HPV39L1的1-469胺基酸C端基因片段重疊的12bp鹼基; 2: HPV59L1 C端的38個(471-508)胺基酸的基因片段; 3: XbaI酶切位點。 |
SEQ ID No: 94 | 3913 HPV 33型L1蛋白的474-499胺基酸序列 KAKPKLKRAAPTSTRTSSAKRKKVKK |
附錄9:序列表-嵌合的人乳頭瘤病毒45型L1蛋白
SEQ ID No: 95 | 4501 HPV 45型L1蛋白的1-478胺基酸序列 MALWRPSDSTVYLPPPSVARVVNTDDYVSRTSIFYHAGSSRLLTVGNPYFRVVPSGAGNKQAVPKVSAYQYRVFRVALPDPNKFGLPDSTIYNPETQRLVWACVGMEIGRGQPLGIGLSGHPFYNKLDDTESAHAATAVITQDVRDNVSVDYKQTQLCILGCVPAIGEHWAKGTLCKPAQLQPGDCPPLELKNTIIEDGDMVDTGYGAMDFSTLQDTKCEVPLDICQSICKYPDYLQMSADPYGDSMFFCLRREQLFARHFWNRAGVMGDTVPTDLYIKGTSANMRETPGSCVYSPSPSGSITTSDSQLFNKPYWLHKAQGHNNGICWHNQLFVTVVDTTRSTNLTLCASTQNPVPNTYDPTKFKHYSRHVEEYDLQFIFQLCTITLTAEVMSYIHSMNSSILENWNFGVPPPPTTSLVDTYRFVQSVAVTCQKDTTPPEKQDPYDKLKFWTVDLKEKFSSDLDQYPLGRKFLVQAGL |
SEQ ID No: 96 | 4502 HPV 33型L1蛋白的474-499胺基酸序列 KAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 97 | 4503 嵌合的HPV 45型L1蛋白的胺基酸序列 MALWRPSDSTVYLPPPSVARVVNTDDYVSRTSIFYHAGSSRLLTVGNPYFRVVPSGAGNKQAVPKVSAYQYRVFRVALPDPNKFGLPDSTIYNPETQRLVWACVGMEIGRGQPLGIGLSGHPFYNKLDDTESAHAATAVITQDVRDNVSVDYKQTQLCILGCVPAIGEHWAKGTLCKPAQLQPGDCPPLELKNTIIEDGDMVDTGYGAMDFSTLQDTKCEVPLDICQSICKYPDYLQMSADPYGDSMFFCLRREQLFARHFWNRAGVMGDTVPTDLYIKGTSANMRETPGSCVYSPSPSGSITTSDSQLFNKPYWLHKAQGHNNGICWHNQLFVTVVDTTRSTNLTLCASTQNPVPNTYDPTKFKHYSRHVEEYDLQFIFQLCTITLTAEVMSYIHSMNSSILENWNFGVPPPPTTSLVDTYRFVQSVAVTCQKDTTPPEKQDPYDKLKFWTVDLKEKFSSDLDQYPLGRKFLVQAGLKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 98 | 4504 嵌合的HPV 45型L1蛋白的核苷酸序列 ATGGCTCTGTGGAGACCATCTGACAGCACAGTCTACCTGCCTCCTCCATCTGTGGCAAGGGTGGTGAACACAGATGACTATGTGAGCAGGACCAGCATCTTCTACCATGCTGGCTCCAGCAGACTGCTGACAGTGGGCAACCCATACTTCAGGGTGGTGCCAAGTGGAGCAGGCAACAAGCAGGCTGTGCCAAAGGTGTCTGCCTACCAATACAGGGTGTTCAGGGTGGCTCTGCCTGACCCAAACAAGTTTGGACTGCCTGACAGCACCATCTACAACCCTGAGACCCAGAGACTGGTGTGGGCTTGTGTGGGGATGGAGATTGGCAGGGGACAACCACTGGGCATTGGACTGTCTGGACACCCATTCTACAACAAACTGGATGACACAGAGTCTGCCCATGCTGCCACAGCAGTGATTACCCAGGATGTGAGGGACAATGTGTCTGTGGACTACAAGCAGACCCAACTTTGTATCCTGGGCTGTGTGCCTGCCATTGGAGAACACTGGGCTAAGGGCACCCTGTGTAAGCCTGCCCAACTCCAACCTGGAGACTGTCCTCCATTGGAACTGAAAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTATGGAGCTATGGACTTCAGCACCCTCCAAGACACCAAGTGTGAGGTGCCACTGGACATCTGTCAGAGCATCTGTAAATACCCTGACTACCTCCAAATGAGTGCTGACCCATATGGAGACAGTATGTTCTTCTGTCTGAGGAGGGAACAACTTTTTGCCAGACACTTCTGGAACAGGGCTGGAGTGATGGGAGACACAGTGCCAACAGACCTCTACATCAAGGGCACCTCTGCCAATATGAGGGAGACACCTGGCTCCTGTGTCTACAGCCCAAGCCCATCTGGCAGCATCACCACCTCTGACAGCCAACTTTTCAACAAGCCATACTGGCTGCACAAGGCTCAAGGACACAACAATGGCATCTGTTGGCACAACCAACTTTTTGTGACAGTGGTGGACACCACCAGGAGCACCAACCTGACCCTGTGTGCCAGCACCCAGAACCCTGTGCCAAACACCTATGACCCAACCAAGTTCAAGCACTACAGCAGGCATGTGGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTACCATCACCCTGACAGCAGAGGTGATGAGTTACATCCACAGTATGAACTCCAGCATCTTGGAGAACTGGAACTTTGGAGTGCCTCCTCCTCCAACCACCTCCCTGGTGGACACCTACAGGTTTGTCCAGTCTGTGGCTGTGACTTGTCAGAAGGACACCACACCTCCTGAGAAGCAGGACCCATATGACAAACTGAAGTTCTGGACAGTGGACCTGAAAGAGAAGTTCTCCTCTGACCTGGACCAATACCCACTGGGCAGGAAGTTCCTGGTCCAGGCTGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAA |
SEQ ID No: 99 | 4505 合成的HPV45L1基因 ctgggtaccATGGCTCTGTGGAGACCATCTGACAGCACAGTCTACCTGCCTCCTCCATCTGTGGCAAGGGTGGTGAACACAGATGACTATGTGAGCAGGACCAGCATCTTCTACCATGCTGGCTCCAGCAGACTGCTGACAGTGGGCAACCCATACTTCAGGGTGGTGCCAAGTGGAGCAGGCAACAAGCAGGCTGTGCCAAAGGTGTCTGCCTACCAATACAGGGTGTTCAGGGTGGCTCTGCCTGACCCAAACAAGTTTGGACTGCCTGACAGCACCATCTACAACCCTGAGACCCAGAGACTGGTGTGGGCTTGTGTGGGGATGGAGATTGGCAGGGGACAACCACTGGGCATTGGACTGTCTGGACACCCATTCTACAACAAACTGGATGACACAGAGTCTGCCCATGCTGCCACAGCAGTGATTACCCAGGATGTGAGGGACAATGTGTCTGTGGACTACAAGCAGACCCAACTTTGTATCCTGGGCTGTGTGCCTGCCATTGGAGAACACTGGGCTAAGGGCACCCTGTGTAAGCCTGCCCAACTCCAACCTGGAGACTGTCCTCCATTGGAACTGAAAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTATGGAGCTATGGACTTCAGCACCCTCCAAGACACCAAGTGTGAGGTGCCACTGGACATCTGTCAGAGCATCTGTAAATACCCTGACTACCTCCAAATGAGTGCTGACCCATATGGAGACAGTATGTTCTTCTGTCTGAGGAGGGAACAACTTTTTGCCAGACACTTCTGGAACAGGGCTGGAGTGATGGGAGACACAGTGCCAACAGACCTCTACATCAAGGGCACCTCTGCCAATATGAGGGAGACACCTGGCTCCTGTGTCTACAGCCCAAGCCCATCTGGCAGCATCACCACCTCTGACAGCCAACTTTTCAACAAGCCATACTGGCTGCACAAGGCTCAAGGACACAACAATGGCATCTGTTGGCACAACCAACTTTTTGTGACAGTGGTGGACACCACCAGGAGCACCAACCTGACCCTGTGTGCCAGCACCCAGAACCCTGTGCCAAACACCTATGACCCAACCAAGTTCAAGCACTACAGCAGGCATGTGGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTACCATCACCCTGACAGCAGAGGTGATGAGTTACATCCACAGTATGAACTCCAGCATCTTGGAGAACTGGAACTTTGGAGTGCCTCCTCCTCCAACCACCTCCCTGGTGGACACCTACAGGTTTGTCCAGTCTGTGGCTGTGACTTGTCAGAAGGACACCACACCTCCTGAGAAGCAGGACCCATATGACAAACTGAAGTTCTGGACAGTGGACCTGAAAGAGAAGTTCTCCTCTGACCTGGACCAATACCCACTGGGCAGGAAGTTCCTGGTCCAGGCTGGACTGAGGAGGAGACCAACCATTGGACCAAGGAAGAGACCTGCTGCCAGCACCAGCACAGCCAGCAGACCTGCCAAGAGGGTGAGGATTAGGAGCAAGAAGTAAA ctcgagctc |
SEQ ID No: 100 | 4506 合成的HPV33L1基因 ctgggtaccATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAActcgagctc |
SEQ ID No: 101 | 4507 HPV45L1 F1 CTTGGTACCATGGCTCTGTGGAGACCATCTGAC |
SEQ ID No: 102 | 4508 HPV45L1 R1 GCTTGGCTTTCAGTCCAGCCTGGACCAGGAAC |
SEQ ID No: 103 | 4509 HPV45L1擴增序列1 CTT GGTACC 1 ATGGCTCTGTGGAGACCATCTGACAGCACAGTCTACCTGCCTCCTCCATCTGTGGCAAGGGTGGTGAACACAGATGACTATGTGAGCAGGACCAGCATCTTCTACCATGCTGGCTCCAGCAGACTGCTGACAGTGGGCAACCCATACTTCAGGGTGGTGCCAAGTGGAGCAGGCAACAAGCAGGCTGTGCCAAAGGTGTCTGCCTACCAATACAGGGTGTTCAGGGTGGCTCTGCCTGACCCAAACAAGTTTGGACTGCCTGACAGCACCATCTACAACCCTGAGACCCAGAGACTGGTGTGGGCTTGTGTGGGGATGGAGATTGGCAGGGGACAACCACTGGGCATTGGACTGTCTGGACACCCATTCTACAACAAACTGGATGACACAGAGTCTGCCCATGCTGCCACAGCAGTGATTACCCAGGATGTGAGGGACAATGTGTCTGTGGACTACAAGCAGACCCAACTTTGTATCCTGGGCTGTGTGCCTGCCATTGGAGAACACTGGGCTAAGGGCACCCTGTGTAAGCCTGCCCAACTCCAACCTGGAGACTGTCCTCCATTGGAACTGAAAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTATGGAGCTATGGACTTCAGCACCCTCCAAGACACCAAGTGTGAGGTGCCACTGGACATCTGTCAGAGCATCTGTAAATACCCTGACTACCTCCAAATGAGTGCTGACCCATATGGAGACAGTATGTTCTTCTGTCTGAGGAGGGAACAACTTTTTGCCAGACACTTCTGGAACAGGGCTGGAGTGATGGGAGACACAGTGCCAACAGACCTCTACATCAAGGGCACCTCTGCCAATATGAGGGAGACACCTGGCTCCTGTGTCTACAGCCCAAGCCCATCTGGCAGCATCACCACCTCTGACAGCCAACTTTTCAACAAGCCATACTGGCTGCACAAGGCTCAAGGACACAACAATGGCATCTGTTGGCACAACCAACTTTTTG TGACAGTGGTGGACACCACCAGGAGCACCAACCTGACCCTGTGTGCCAGCACCCAGAACCCTGTGCCAAACACCTATGACCCAACCAAGTTCAAGCACTACAGCAGGCATGTGGAGGAATATGACCTCCAATTCATCTTCCAACTTTGTACCATCACCCTGACAGCAGAGGTGATGAGTTACATCCACAGTATGAACTCCAGCATCTTGGAGAACTGGAACTTTGGAGTGCCTCCTCCTCCAACCACCTCCCTGGTGGACACCTACAGGTTTGTCCAGTCTGTGGCTGTGACTTGTCAGAAGGACACCACACCTCCTGAGAAGCAGGACCCATATGACAAACTGAAGTTCTGGACAGTGGACCTGAAAGAGAAGTTCTCCTCTGACCTGGACCAATACCCACTGGGCAGGAAGTTCCTGGTCCAGGCTGGACTG 2 AAAGCCAAGC 31: KpnⅠ酶切位點; 2: 編碼HPV45L1的1-478胺基酸的基因片段; 3: 與HPV33L1的474-499胺基酸的基因片段重疊的10個鹼基. |
SEQ ID No: 104 | 4510 HPV45L1 F2 GGCTGGACTGAAAGCCAAGCCAAAACTGAAAAGGG |
SEQ ID No: 105 | 4511 HPV45L1 R2 CTGTCTAGATTTACTTCTTCACCTTCTTCCTCTTGGC |
SEQ ID No: 106 | 4512 HPV45L1擴增序列2 GGCTGGACTG 1 AAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGG ACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAG 2TAA A TCTAGA 3CAG. 1: 與HPV45L1的1-478胺基酸C端基因片段重疊的10bp鹼基; 2: HPV33L1 C端的26個(474-499)胺基酸的基因片段; 3: XbaI酶切位點. |
SEQ ID No: 107 | 4513 HPV 59型L1蛋白的471-508胺基酸序列 LQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
附錄10:序列表-嵌合的人乳頭瘤病毒51型L1蛋白
SEQ ID No: 108 | 5101 HPV 51型L1蛋白的1-474胺基酸序列 MALWRTNDSKVYLPPAPVSRIVNTEEYITRTGIYYYAGSSRLITLGHPYFPIPKTSTRAAIPKVSAFQYRVFRVQLPDPNKFGLPDPNLYNPDTDRLVWGCVGVEVGRGQPLGVGLSGHPLFNKYDDTENSRIANGNAQQDVRDNTSVDNKQTQLCIIGCAPPIGEHWGIGTTCKNTPVPPGDCPPLELVSSVIQDGDMIDTGFGAMDFAALQATKSDVPLDISQSVCKYPDYLKMSADTYGNSMFFHLRREQIFARHYYNKLVGVGEDIPNDYYIKGSGNGRDPIESYIYSATPSGSMITSDSQIFNKPYWLHRAQGHNNGICWNNQLFITCVDTTRSTNLTISTATAAVSPTFTPSNFKQYIRHGEEYELQFIFQLCKITLTTEVMAYLHTMDPTILEQWNFGLTLPPSASLEDAYRFVRNAATSCQKDTPPQAKPDPLAKYKFWDVDLKERFSLDLDQFALGRKFLLQVGV |
SEQ ID No: 109 | 5102 HPV 33型L1蛋白的474-499胺基酸序列 KAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 110 | 5103 嵌合的HPV 51型L1蛋白的胺基酸序列 MALWRTNDSKVYLPPAPVSRIVNTEEYITRTGIYYYAGSSRLITLGHPYFPIPKTSTRAAIPKVSAFQYRVFRVQLPDPNKFGLPDPNLYNPDTDRLVWGCVGVEVGRGQPLGVGLSGHPLFNKYDDTENSRIANGNAQQDVRDNTSVDNKQTQLCIIGCAPPIGEHWGIGTTCKNTPVPPGDCPPLELVSSVIQDGDMIDTGFGAMDFAALQATKSDVPLDISQSVCKYPDYLKMSADTYGNSMFFHLRREQIFARHYYNKLVGVGEDIPNDYYIKGSGNGRDPIESYIYSATPSGSMITSDSQIFNKPYWLHRAQGHNNGICWNNQLFITCVDTTRSTNLTISTATAAVSPTFTPSNFKQYIRHGEEYELQFIFQLCKITLTTEVMAYLHTMDPTILEQWNFGLTLPPSASLEDAYRFVRNAATSCQKDTPPQAKPDPLAKYKFWDVDLKERFSLDLDQFALGRKFLLQVGVKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 111 | 5104 嵌合的HPV 51型L1蛋白的核苷酸序列 ATGGCTCTGTGGAGGACCAATGACAGCAAGGTCTACCTGCCTCCTGCCCCTGTGAGCAGGATTGTGAACACAGAGGAATACATCACCAGGACAGGCATCTACTACTATGCTGGCTCCAGCAGACTGATTACCCTGGGACACCCATACTTTCCAATCCCAAAGACCAGCACCAGGGCTGCCATCCCAAAGGTGTCTGCCTTCCAATACAGGGTGTTCAGGGTCCAACTTCCTGACCCAAACAAGTTTGGACTGCCTGACCCAAACCTCTACAACCCTGACACAGACAGACTGGTGTGGGGCTGTGTGGGAGTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGACTGTCTGGACACCCACTGTTCAACAAATATGATGACACAGAGAACAGCAGGATTGCCAATGGCAATGCCCAACAGGATGTGAGGGACAACACCTCTGTGGACAACAAGCAGACCCAACTTTGTATCATTGGCTGTGCCCCTCCAATTGGAGAACACTGGGGCATTGGCACCACTTGTAAGAACACACCTGTGCCTCCTGGAGACTGTCCTCCATTGGAACTGGTGTCCTCTGTGATTCAGGATGGAGATATGATTGACACAGGCTTTGGAGCTATGGACTTTGCTGCCCTCCAAGCCACCAAGTCTGATGTGCCACTGGACATCAGCCAGTCTGTGTGTAAATACCCTGACTACCTGAAAATGAGTGCTGACACCTATGGCAACAGTATGTTCTTCCACCTGAGGAGGGAACAGATTTTTGCCAGACACTACTACAACAAACTGGTGGGAGTGGGAGAGGACATCCCAAATGACTACTACATCAAGGGCTCTGGCAATGGCAGGGACCCAATTGAGTCCTACATCTACTCTGCCACACCATCTGGCAGTATGATTACCTCTGACAGCCAGATTTTCAACAAGCCATACTGGCTGCACAGGGCTCAAGGACACAACAATGGCATCTGTTGGAACAACCAACTTTTCATCACTTGTGTGGACACCACCAGGAGCACCAACCTGACCATCAGCACAGCCACAGCAGCAGTGAGCCCAACCTTCACACCAAGCAACTTCAAGCAATACATCAGACATGGAGAGGAATATGAACTCCAATTCATCTTCCAACTTTGTAAGATTACCCTGACCACAGAGGTGATGGCTTACCTGCACACAATGGACCCAACCATCTTGGAACAGTGGAACTTTGGACTGACCCTGCCTCCATCTGCCTCCTTGGAGGATGCCTACAGGTTTGTGAGGAATGCTGCCACCTCCTGTCAGAAGGACACACCTCCACAGGCTAAGCCTGACCCACTGGCTAAATACAAGTTCTGGGATGTGGACCTGAAAGAGAGGTTCTCCCTGGACCTGGACCAGTTTGCCCTGGGCAGGAAGTTCCTGCTCCAAGTGGGAGTCAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAA |
SEQ ID No: 112 | 5105 合成的HPV51L1基因 ctgggtaccATGGCTCTGTGGAGGACCAATGACAGCAAGGTCTACCTGCCTCCTGCCCCTGTGAGCAGGATTGTGAACACAGAGGAATACATCACCAGGACAGGCATCTACTACTATGCTGGCTCCAGCAGACTGATTACCCTGGGACACCCATACTTTCCAATCCCAAAGACCAGCACCAGGGCTGCCATCCCAAAGGTGTCTGCCTTCCAATACAGGGTGTTCAGGGTCCAACTTCCTGACCCAAACAAGTTTGGACTGCCTGACCCAAACCTCTACAACCCTGACACAGACAGACTGGTGTGGGGCTGTGTGGGAGTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGACTGTCTGGACACCCACTGTTCAACAAATATGATGACACAGAGAACAGCAGGATTGCCAATGGCAATGCCCAACAGGATGTGAGGGACAACACCTCTGTGGACAACAAGCAGACCCAACTTTGTATCATTGGCTGTGCCCCTCCAATTGGAGAACACTGGGGCATTGGCACCACTTGTAAGAACACACCTGTGCCTCCTGGAGACTGTCCTCCATTGGAACTGGTGTCCTCTGTGATTCAGGATGGAGATATGATTGACACAGGCTTTGGAGCTATGGACTTTGCTGCCCTCCAAGCCACCAAGTCTGATGTGCCACTGGACATCAGCCAGTCTGTGTGTAAATACCCTGACTACCTGAAAATGAGTGCTGACACCTATGGCAACAGTATGTTCTTCCACCTGAGGAGGGAACAGATTTTTGCCAGACACTACTACAACAAACTGGTGGGAGTGGGAGAGGACATCCCAAATGACTACTACATCAAGGGCTCTGGCAATGGCAGGGACCCAATTGAGTCCTACATCTACTCTGCCACACCATCTGGCAGTATGATTACCTCTGACAGCCAGATTTTCAACAAGCCATACTGGCTGCACAGGGCTCAAGGACACAACAATGGCATCTGTTGGAACAACCAACTTTTCATCACTTGTGTGGACACCACCAGGAGCACCAACCTGACCATCAGCACAGCCACAGCAGCAGTGAGCCCAACCTTCACACCAAGCAACTTCAAGCAATACATCAGACATGGAGAGGAATATGAACTCCAATTCATCTTCCAACTTTGTAAGATTACCCTGACCACAGAGGTGATGGCTTACCTGCACACAATGGACCCAACCATCTTGGAACAGTGGAACTTTGGACTGACCCTGCCTCCATCTGCCTCCTTGGAGGATGCCTACAGGTTTGTGAGGAATGCTGCCACCTCCTGTCAGAAGGACACACCTCCACAGGCTAAGCCTGACCCACTGGCTAAATACAAGTTCTGGGATGTGGACCTGAAAGAGAGGTTCTCCCTGGACCTGGACCAGTTTGCCCTGGGCAGGAAGTTCCTGCTCCAAGTGGGAGTCCAGAGGAAGCCAAGACCTGGACTGAAAAGACCTGCCTCCTCTGCCTCCTCCTCCTCCTCCTCCTCTGCCAAGAGGAAGAGGGTGAAGAAGTAAActcgagctc |
SEQ ID No: 113 | 5106 合成的HPV33L1基因 ctgggtaccATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAActcgagctc |
SEQ ID No: 114 | 5107 HPV51L1 F1 CTTGGTACCATGGCTCTGTGGAGGACCAATGACA |
SEQ ID No: 115 | 5108 HPV51L1 R1 GCTTGGCTTTGACTCCCACTTGGAGCAGGAAC |
SEQ ID No: 116 | 5109 HPV51L1擴增序列1 CTT GGTACC 1 ATGGCTCTGTGGAGGACCAATGACAGCAAGGTCTACCTGCCTCCTGCCCCTGTGAGCAGGATTGTGAACACAGAGGAATACATCACCAGGACAGGCATCTACTACTATGCTGGCTCCAGCAGACTGATTACCCTGGGACACCCATACTTTCCAATCCCAAAGACCAGCACCAGGGCTGCCATCCCAAAGGTGTCTGCCTTCCAATACAGGGTGTTCAGGGTCCAACTTCCTGACCCAAACAAGTTTGGACTGCCTGACCCAAACCTCTACAACCCTGACACAGACAGACTGGTGTGGGGCTGTGTGGGAGTGGAGGTGGGCAGGGGACAACCACTGGGAGTGGGACTGTCTGGACACCCACTGTTCAACAAATATGATGACACAGAGAACAGCAGGATTGCCAATGGCAATGCCCAACAGGATGTGAGGGACAACACCTCTGTGGACAACAAGCAGACCCAACTTTGTATCATTGGCTGTGCCCCTCCAATTGGAGAACACTGGGGCATTGGCACCACTTGTAAGAACACACCTGTGCCTCCTGGAGACTGTCCTCCATTGGAACTGGTGTCCTCTGTGATTCAGGATGGAGATATGATTGACACAGGCTTTGGAGCTATGGACTTTGCTGCCCTCCAAGCCACCAAGTCTGATGTGCCACTGGACATCAGCCAGTCTGTGTGTAAATACCCTGACTACCTGAAAATGAGTGCTGACACCTATGGCAACAGTATGTTCTTCCACCTGAGGAGGGAACAGATTTTTGCCAGACACTACTACAACAAACTGGTGGGAGTGGGAGAGGACATCCCAAATGACTACTACATCAAGGGCTCTGGCAATGGCAGGGACCCAATTGAGTCCTACATCTACTCTGCCACACCATCTGGCAGTATGATTACCTCTGACAGCCAGATTTTCAACAAGCCATACTGGCTGCACAGGGCTCAAGGACACAACAATGGCATCTGTTGGAACAACCAACTTTTCATCACTTGTG TGGACACCACCAGGAGCACCAACCTGACCATCAGCACAGCCACAGCAGCAGTGAGCCCAACCTTCACACCAAGCAACTTCAAGCAATACATCAGACATGGAGAGGAATATGAACTCCAATTCATCTTCCAACTTTGTAAGATTACCCTGACCACAGAGGTGATGGCTTACCTGCACACAATGGACCCAACCATCTTGGAACAGTGGAACTTTGGACTGACCCTGCCTCCATCTGCCTCCTTGGAGGATGCCTACAGGTTTGTGAGGAATGCTGCCACCTCCTGTCAGAAGGACACACCTCCACAGGCTAAGCCTGACCCACTGGCTAAATACAAGTTCTGGGATGTGGACCTGAAAGAGAGGTTCTCCCTGGACCTGGACCAGTTTGCCCTGGGCAGGAAGTTCCTGCTCCAAGTGGGAGTC 2 AAAGCCAAGC 31: KpnⅠ酶切位點; 2: 編碼HPV51L1的1-474胺基酸的基因片段; 3: 與HPV33L1的474-499胺基酸的基因片段重疊的10個鹼基 |
SEQ ID No: 117 | 5110 HPV51L1 F2 AGTGGGAGTCAAAGCCAAGCCAAAACTGAAAAGGG |
SEQ ID No: 118 | 5111 HPV51L1 R2 CTGTCTAGATTTACTTCTTCACCTTCTTCCTCTTGG |
SEQ ID No: 119 | 5112 HPV51L1擴增序列2 AGTGGGAGTC 1 AAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGG ACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAG 2TAA A TCTAGA 3CAG. 1: 與HPV51L1的1-474胺基酸C端基因片段重疊的10bp鹼基; 2: HPV33L1 C端的26個(474-499)胺基酸的基因片段; 3: XbaI酶切位點. |
SEQ ID No: 120 | 5113 HPV 59型L1蛋白的471-508胺基酸序列 LQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
附錄11:序列表-嵌合的人乳頭瘤病毒52型L1蛋白
SEQ ID No: 121 | 5201 HPV 52型L1蛋白的1-478胺基酸序列 MSVWRPSEATVYLPPVPVSKVVSTDEYVSRTSIYYYAGSSRLLTVGHPYFSIKNTSSGNGKKVLVPKVSGLQYRVFRIKLPDPNKFGFPDTSFYNPETQRLVWACTGLEIGRGQPLGVGISGHPLLNKFDDTETSNKYAGKPGIDNRECLSMDYKQTQLCILGCKPPIGEHWGKGTPCNNNSGNPGDCPPLQLINSVIQDGDMVDTGFGCMDFNTLQASKSDVPIDICSSVCKYPDYLQMASEPYGDSLFFFLRREQMFVRHFFNRAGTLGDPVPGDLYIQGSNSGNTATVQSSAFFPTPSGSMVTSESQLFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMTLCAEVKKESTYKNENFKEYLRHGEEFDLQFIFQLCKITLTADVMTYIHKMDATILEDWQFGLTPPPSASLEDTYRFVTSTAITCQKNTPPKGKEDPLKDYMFWEVDLKEKFSADLDQFPLGRKFLLQAGL |
SEQ ID No: 122 | 5202 HPV 33型L1蛋白的474-499胺基酸序列 KAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 123 | 5203 嵌合的HPV 52型L1蛋白的胺基酸序列 MSVWRPSEATVYLPPVPVSKVVSTDEYVSRTSIYYYAGSSRLLTVGHPYFSIKNTSSGNGKKVLVPKVSGLQYRVFRIKLPDPNKFGFPDTSFYNPETQRLVWACTGLEIGRGQPLGVGISGHPLLNKFDDTETSNKYAGKPGIDNRECLSMDYKQTQLCILGCKPPIGEHWGKGTPCNNNSGNPGDCPPLQLINSVIQDGDMVDTGFGCMDFNTLQASKSDVPIDICSSVCKYPDYLQMASEPYGDSLFFFLRREQMFVRHFFNRAGTLGDPVPGDLYIQGSNSGNTATVQSSAFFPTPSGSMVTSESQLFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMTLCAEVKKESTYKNENFKEYLRHGEEFDLQFIFQLCKITLTADVMTYIHKMDATILEDWQFGLTPPPSASLEDTYRFVTSTAITCQKNTPPKGKEDPLKDYMFWEVDLKEKFSADLDQFPLGRKFLLQAGLKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 124 | 5204 嵌合的HPV 52型L1蛋白的核苷酸序列 ATGAGCGTGTGGAGGCCCAGCGAGGCCACCGTGTACCTGCCCCCCGTGCCCGTGAGCAAGGTGGTGAGCACCGACGAGTACGTGAGCAGGACCAGCATCTACTACTACGCCGGCAGCAGCAGGCTGCTGACCGTGGGCCACCCCTACTTCAGCATCAAGAACACCAGCAGCGGCAACGGCAAGAAGGTGCTGGTGCCCAAGGTGAGCGGCCTGCAGTACAGGGTGTTCAGGATCAAGCTGCCCGACCCCAACAAGTTCGGCTTCCCCGACACCAGCTTCTACAACCCCGAGACCCAGAGGCTGGTGTGGGCCTGCACCGGCCTGGAGATCGGCAGGGGCCAGCCCCTGGGCGTGGGCATCAGCGGCCACCCCCTGCTGAACAAGTTCGACGACACCGAGACCAGCAACAAGTACGCCGGCAAGCCCGGCATCGACAACAGGGAGTGCCTGAGCATGGACTACAAGCAGACCCAGCTGTGCATCCTGGGCTGCAAGCCCCCCATCGGCGAGCACTGGGGCAAGGGCACCCCCTGCAACAACAACAGCGGCAACCCCGGCGACTGCCCCCCCCTGCAGCTGATCAACAGCGTGATCCAGGACGGCGACATGGTGGACACCGGCTTCGGCTGCATGGACTTCAACACCCTGCAGGCCAGCAAGAGCGACGTGCCCATCGACATCTGCAGCAGCGTGTGCAAGTACCCCGACTACCTGCAGATGGCCAGCGAGCCCTACGGCGACAGCCTGTTCTTCTTCCTGAGGAGGGAGCAGATGTTCGTGAGGCACTTCTTCAACAGGGCCGGCACCCTGGGCGACCCCGTGCCCGGCGACCTGTACATCCAGGGCAGCAACAGCGGCAACACCGCCACCGTGCAGAGCAGCGCCTTCTTCCCCACCCCCAGCGGCAGCATGGTGACCAGCGAGAGCCAGCTGTTCAACAAGCCCTACTGGCTGCAGAGGGCCCAGGGCCACAACAACGGCATCTGCTGGGGCAACCAGCTGTTCGTGACCGTGGTGGACACCACCAGGAGCACCAACATGACCCTGTGCGCCGAGGTGAAGAAGGAGAGCACCTACAAGAACGAGAACTTCAAGGAGTACCTGAGGCACGGCGAGGAGTTCGACCTGCAGTTCATCTTCCAGCTGTGCAAGATCACCCTGACCGCCGACGTGATGACCTACATCCACAAGATGGACGCCACCATCCTGGAGGACTGGCAGTTCGGCCTGACCCCCCCCCCCAGCGCCAGCCTGGAGGACACCTACAGGTTCGTGACCAGCACCGCCATCACCTGCCAGAAGAACACCCCCCCCAAGGGCAAGGAGGACCCCCTGAAGGACTACATGTTCTGGGAGGTGGACCTGAAGGAGAAGTTCAGCGCCGACCTGGACCAGTTCCCCCTGGGCAGGAAGTTCCTGCTGCAGGCCGGCCTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAA |
SEQ ID No: 125 | 5205 合成的HPV52L1基因 ctgggtaccATGAGCGTGTGGAGGCCCAGCGAGGCCACCGTGTACCTGCCCCCCGTGCCCGTGAGCAAGGTGGTGAGCACCGACGAGTACGTGAGCAGGACCAGCATCTACTACTACGCCGGCAGCAGCAGGCTGCTGACCGTGGGCCACCCCTACTTCAGCATCAAGAACACCAGCAGCGGCAACGGCAAGAAGGTGCTGGTGCCCAAGGTGAGCGGCCTGCAGTACAGGGTGTTCAGGATCAAGCTGCCCGACCCCAACAAGTTCGGCTTCCCCGACACCAGCTTCTACAACCCCGAGACCCAGAGGCTGGTGTGGGCCTGCACCGGCCTGGAGATCGGCAGGGGCCAGCCCCTGGGCGTGGGCATCAGCGGCCACCCCCTGCTGAACAAGTTCGACGACACCGAGACCAGCAACAAGTACGCCGGCAAGCCCGGCATCGACAACAGGGAGTGCCTGAGCATGGACTACAAGCAGACCCAGCTGTGCATCCTGGGCTGCAAGCCCCCCATCGGCGAGCACTGGGGCAAGGGCACCCCCTGCAACAACAACAGCGGCAACCCCGGCGACTGCCCCCCCCTGCAGCTGATCAACAGCGTGATCCAGGACGGCGACATGGTGGACACCGGCTTCGGCTGCATGGACTTCAACACCCTGCAGGCCAGCAAGAGCGACGTGCCCATCGACATCTGCAGCAGCGTGTGCAAGTACCCCGACTACCTGCAGATGGCCAGCGAGCCCTACGGCGACAGCCTGTTCTTCTTCCTGAGGAGGGAGCAGATGTTCGTGAGGCACTTCTTCAACAGGGCCGGCACCCTGGGCGACCCCGTGCCCGGCGACCTGTACATCCAGGGCAGCAACAGCGGCAACACCGCCACCGTGCAGAGCAGCGCCTTCTTCCCCACCCCCAGCGGCAGCATGGTGACCAGCGAGAGCCAGCTGTTCAACAAGCCCTACTGGCTGCAGAGGGCCCAGGGCCACAACAACGGCATCTGCTGGGGCAACCAGCTGTTCGTGACCGTGGTGGACACCACCAGGAGCACCAACATGACCCTGTGCGCCGAGGTGAAGAAGGAGAGCACCTACAAGAACGAGAACTTCAAGGAGTACCTGAGGCACGGCGAGGAGTTCGACCTGCAGTTCATCTTCCAGCTGTGCAAGATCACCCTGACCGCCGACGTGATGACCTACATCCACAAGATGGACGCCACCATCCTGGAGGACTGGCAGTTCGGCCTGACCCCCCCCCCCAGCGCCAGCCTGGAGGACACCTACAGGTTCGTGACCAGCACCGCCATCACCTGCCAGAAGAACACCCCCCCCAAGGGCAAGGAGGACCCCCTGAAGGACTACATGTTCTGGGAGGTGGACCTGAAGGAGAAGTTCAGCGCCGACCTGGACCAGTTCCCCCTGGGCAGGAAGTTCCTGCTGCAGGCCGGCCTGCAGGCCAGGCCCAAGCTGAAGAGGCCCGCCAGCAGCGCCCCCAGGACCAGCACCAAGAAGAAGAAGGTGAAGAGGTAAActcgagctc |
SEQ ID No: 126 | 5206 合成的HPV33L1基因 ctgggtaccATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAActcgagctc |
SEQ ID No: 127 | 5207 HPV52L1 F1 CTTGGTACCATGAGCGTGTGGAGGCCCAGCGAGG |
SEQ ID No: 128 | 5208 HPV52L1 R1 GCTTGGCTTTCAGGCCGGCCTGCAGCAGGAACTTC |
SEQ ID No: 129 | 5209 HPV52L1擴增序列1 CTT GGTACC 1 ATGAGCGTGTGGAGGCCCAGCGAGGCCACCGTGTACCTGCCCCCCGTGCCCGTGAGCAAGGTGGTGAGCACCGACGAGTACGTGAGCAGGACCAGCATCTACTACTACGCCGGCAGCAGCAGGCTGCTGACCGTGGGCCACCCCTACTTCAGCATCAAGAACACCAGCAGCGGCAACGGCAAGAAGGTGCTGGTGCCCAAGGTGAGCGGCCTGCAGTACAGGGTGTTCAGGATCAAGCTGCCCGACCCCAACAAGTTCGGCTTCCCCGACACCAGCTTCTACAACCCCGAGACCCAGAGGCTGGTGTGGGCCTGCACCGGCCTGGAGATCGGCAGGGGCCAGCCCCTGGGCGTGGGCATCAGCGGCCACCCCCTGCTGAACAAGTTCGACGACACCGAGACCAGCAACAAGTACGCCGGCAAGCCCGGCATCGACAACAGGGAGTGCCTGAGCATGGACTACAAGCAGACCCAGCTGTGCATCCTGGGCTGCAAGCCCCCCATCGGCGAGCACTGGGGCAAGGGCACCCCCTGCAACAACAACAGCGGCAACCCCGGCGACTGCCCCCCCCTGCAGCTGATCAACAGCGTGATCCAGGACGGCGACATGGTGGACACCGGCTTCGGCTGCATGGACTTCAACACCCTGCAGGCCAGCAAGAGCGACGTGCCCATCGACATCTGCAGCAGCGTGTGCAAGTACCCCGACTACCTGCAGATGGCCAGCGAGCCCTACGGCGACAGCCTGTTCTTCTTCCTGAGGAGGGAGCAGATGTTCGTGAGGCACTTCTTCAACAGGGCCGGCACCCTGGGCGACCCCGTGCCCGGCGACCTGTACATCCAGGGCAGCAACAGCGGCAACACCGCCACCGTGCAGAGCAGCGCCTTCTTCCCCACCCCCAGCGGCAGCATGGTGACCAGCGAGAGCCAGCTGTTCAACAAGCCCTACTGGCTGCAGAGGGCCCAGGGCCACAACAACGGCATCTGCTGGGGCAACCAGCTGTTCGTGACCGTGGTGGACACCACCAGGAGCACCAACATGACCCTGTGCGCCGAGGTGAAGAAGGAGAGCACCTACAAGAACGAGAACTTCAAGGAGTACCTGAGGCACGGCGAGGAGTTCGACCTGCAGTTCATCTTCCAGCTGTGCAAGATCACCCTGACCGCCGACGTGATGACCTACATCCACAAGATGGACGCCACCATCCTGGAGGACTGGCAGTTCGGCCTGACCCCCCCCCCCAGCGCCAGCCTGGAGGACACCTACAGGTTCGTGACCAGCACCGCCATCACCTGCCAGAAGAACACCCCCCCCAAGGGCAAGGAGGACCCCCTGAAGGACTACATGTTCTGGGAGGTGGACCTGAAGGAGAAGTTCAGCGCCGACCTGGACCAGTTCCCCCTGGGCAGGAAGTTCCTGCTGCAGGCCGGCCTG 2 AAAGCCAAGC 31: KpnⅠ酶切位點; 2: 編碼HPV52L1的1-478胺基酸的基因片段; 3: 與HPV33L1的474-499胺基酸的基因片段重疊的10個鹼基。 |
SEQ ID No: 130 | 5210 HPV52L1 F2 GGCCGGCCTGAAAGCCAAGCCAAAACTGAAAAGGG |
SEQ ID No: 131 | 5211 HPV52L1 R2 CTGTCTAGATTTACTTCTTCACCTTCTTCCTCTTGG |
SEQ ID No: 132 | 5212 HPV52L1擴增序列2 GGCCGGCCTG 1 AAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGG ACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAG 2TAA A TCTAGA 3CAG. 1: 與HPV52L1的1-478胺基酸C端基因片段重疊的10bp鹼基; 2: HPV33L1 C端的26個(474-499)胺基酸的基因片段; 3: XbaI酶切位點 |
SEQ ID No: 133 | 5213 HPV 59型L1蛋白的471-508胺基酸序列 LQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
附錄12:序列表-嵌合的人乳頭瘤病毒56型L1蛋白
SEQ ID No: 134 | 5601 HPV 56型L1蛋白的1-467胺基酸序列 MATWRPSENKVYLPPTPVSKVVATDSYVKRTSIFYHAGSSRLLAVGHPYYSVTKDNTKTNIPKVSAYQYRVFRVRLPDPNKFGLPDTNIYNPDQERLVWACVGLEVGRGQPLGAGLSGHPLFNRLDDTESSNLANNNVIEDSRDNISVDGKQTQLCIVGCTPAMGEHWTKGAVCKSTQVTTGDCPPLALINTPIEDGDMIDTGFGAMDFKVLQESKAEVPLDIVQSTCKYPDYLKMSADAYGDSMWFYLRREQLFARHYFNRAGKVGETIPAELYLKGSNGREPPPSSVYVATPSGSMITSEAQLFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMTISTATEQLSKYDARKINQYLRHVEEYELQFVFQLCKITLSAEVMAYLHNMNANLLEDWNIGLSPPVATSLEDKYRYVRSTAITCQREQPPTEKQDPLAKYKFWDVNLQDSFSTDLDQFPLGRKFL |
SEQ ID No: 135 | 5602 HPV 33型L1蛋白的469-499胺基酸序列 LQAGLKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 136 | 5603 嵌合的HPV 56型L1蛋白的胺基酸序列 MATWRPSENKVYLPPTPVSKVVATDSYVKRTSIFYHAGSSRLLAVGHPYYSVTKDNTKTNIPKVSAYQYRVFRVRLPDPNKFGLPDTNIYNPDQERLVWACVGLEVGRGQPLGAGLSGHPLFNRLDDTESSNLANNNVIEDSRDNISVDGKQTQLCIVGCTPAMGEHWTKGAVCKSTQVTTGDCPPLALINTPIEDGDMIDTGFGAMDFKVLQESKAEVPLDIVQSTCKYPDYLKMSADAYGDSMWFYLRREQLFARHYFNRAGKVGETIPAELYLKGSNGREPPPSSVYVATPSGSMITSEAQLFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMTISTATEQLSKYDARKINQYLRHVEEYELQFVFQLCKITLSAEVMAYLHNMNANLLEDWNIGLSPPVATSLEDKYRYVRSTAITCQREQPPTEKQDPLAKYKFWDVNLQDSFSTDLDQFPLGRKFLLQAGLKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 137 | 5604 嵌合的HPV 56型L1蛋白的核苷酸序列 ATGGCTACCTGGAGACCATCTGAGAACAAGGTCTACCTGCCTCCAACACCTGTGAGCAAGGTGGTGGCTACAGACTCCTATGTGAAGAGGACCAGCATCTTCTACCATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTACTCTGTGACCAAGGACAACACCAAGACCAACATCCCAAAGGTGTCTGCCTACCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGACTGCCTGACACCAACATCTACAACCCTGACCAGGAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGGTGGGCAGGGGACAACCACTGGGAGCAGGACTGTCTGGACACCCACTGTTCAACAGACTGGATGACACAGAGTCCAGCAACCTGGCTAACAACAATGTGATTGAGGACAGCAGGGACAACATCTCTGTGGATGGCAAGCAGACCCAACTTTGTATTGTGGGCTGTACTCCTGCTATGGGAGAACACTGGACCAAGGGAGCAGTGTGTAAGAGCACCCAGGTGACCACAGGAGACTGTCCTCCACTGGCTCTGATAAACACACCAATTGAGGATGGAGATATGATTGACACAGGCTTTGGAGCTATGGACTTCAAGGTGCTCCAAGAGAGCAAGGCTGAGGTGCCACTGGACATTGTCCAGAGCACTTGTAAATACCCTGACTACCTGAAAATGAGTGCTGATGCCTATGGAGACAGTATGTGGTTCTACCTGAGGAGGGAACAACTTTTTGCCAGACACTACTTCAACAGGGCTGGCAAGGTGGGAGAGACCATCCCTGCTGAACTCTACCTGAAAGGCAGCAATGGCAGGGAACCTCCTCCATCCTCTGTCTATGTGGCTACACCATCTGGCAGTATGATTACCTCTGAGGCTCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAACTTTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCATCAGCACAGCCACAGAACAACTTAGCAAATATGATGCCAGGAAGATAAACCAATACCTGAGGCATGTGGAGGAATATGAACTCCAATTTGTGTTCCAACTTTGTAAGATTACCCTGTCTGCTGAGGTGATGGCTTACCTGCACAATATGAATGCCAACCTGTTGGAGGACTGGAACATTGGACTGAGCCCTCCTGTGGCTACCTCCTTGGAGGACAAATACAGATATGTGAGGAGCACAGCCATCACTTGTCAGAGGGAACAACCTCCAACAGAGAAGCAGGACCCACTGGCTAAATACAAGTTCTGGGATGTGAACCTCCAAGACTCCTTCAGCACAGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAA |
SEQ ID No: 138 | 5605 合成的HPV56L1基因 ctgggtaccATGGCTACCTGGAGACCATCTGAGAACAAGGTCTACCTGCCTCCAACACCTGTGAGCAAGGTGGTGGCTACAGACTCCTATGTGAAGAGGACCAGCATCTTCTACCATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTACTCTGTGACCAAGGACAACACCAAGACCAACATCCCAAAGGTGTCTGCCTACCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGACTGCCTGACACCAACATCTACAACCCTGACCAGGAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGGTGGGCAGGGGACAACCACTGGGAGCAGGACTGTCTGGACACCCACTGTTCAACAGACTGGATGACACAGAGTCCAGCAACCTGGCTAACAACAATGTGATTGAGGACAGCAGGGACAACATCTCTGTGGATGGCAAGCAGACCCAACTTTGTATTGTGGGCTGTACTCCTGCTATGGGAGAACACTGGACCAAGGGAGCAGTGTGTAAGAGCACCCAGGTGACCACAGGAGACTGTCCTCCACTGGCTCTGATAAACACACCAATTGAGGATGGAGATATGATTGACACAGGCTTTGGAGCTATGGACTTCAAGGTGCTCCAAGAGAGCAAGGCTGAGGTGCCACTGGACATTGTCCAGAGCACTTGTAAATACCCTGACTACCTGAAAATGAGTGCTGATGCCTATGGAGACAGTATGTGGTTCTACCTGAGGAGGGAACAACTTTTTGCCAGACACTACTTCAACAGGGCTGGCAAGGTGGGAGAGACCATCCCTGCTGAACTCTACCTGAAAGGCAGCAATGGCAGGGAACCTCCTCCATCCTCTGTCTATGTGGCTACACCATCTGGCAGTATGATTACCTCTGAGGCTCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAACTTTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCATCAGCACAGCCACAGAACAACTTAGCAAATATGATGCCAGGAAGATAAACCAATACCTGAGGCATGTGGAGGAATATGAACTCCAATTTGTGTTCCAACTTTGTAAGATTACCCTGTCTGCTGAGGTGATGGCTTACCTGCACAATATGAATGCCAACCTGTTGGAGGACTGGAACATTGGACTGAGCCCTCCTGTGGCTACCTCCTTGGAGGACAAATACAGATATGTGAGGAGCACAGCCATCACTTGTCAGAGGGAACAACCTCCAACAGAGAAGCAGGACCCACTGGCTAAATACAAGTTCTGGGATGTGAACCTCCAAGACTCCTTCAGCACAGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGATGCAACTTGGCACCAGGAGCAAGCCTGCTGTGGCTACCAGCAAGAAGAGGTCTGCCCCAACCAGCACCAGCACACCTGCCAAGAGGAAGAGGAGGTAAActcgagctc |
SEQ ID No: 139 | 5606 合成的HPV33L1基因 ctgggtaccATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAActcgagctc |
SEQ ID No: 140 | 5607 HPV56L1 F1 CTTGGTACCATGGCTACCTGGAGACCATCTGAG |
SEQ ID No: 141 | 5608 HPV56L1 R1 CTGCTTGGAGCAGGAACTTCCTGCCCAGTGG |
SEQ ID No: 142 | 5609 HPV56L1擴增序列1 CTT GGTACC 1 ATGGCTACCTGGAGACCATCTGAGAACAAGGTCTACCTGCCTCCAACACCTGTGAGCAAGGTGGTGGCTACAGACTCCTATGTGAAGAGGACCAGCATCTTCTACCATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTACTCTGTGACCAAGGACAACACCAAGACCAACATCCCAAAGGTGTCTGCCTACCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGACTGCCTGACACCAACATCTACAACCCTGACCAGGAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGGTGGGCAGGGGACAACCACTGGGAGCAGGACTGTCTGGACACCCACTGTTCAACAGACTGGATGACACAGAGTCCAGCAACCTGGCTAACAACAATGTGATTGAGGACAGCAGGGACAACATCTCTGTGGATGGCAAGCAGACCCAACTTTGTATTGTGGGCTGTACTCCTGCTATGGGAGAACACTGGACCAAGGGAGCAGTGTGTAAGAGCACCCAGGTGACCACAGGAGACTGTCCTCCACTGGCTCTGATAAACACACCAATTGAGGATGGAGATATGATTGACACAGGCTTTGGAGCTATGGACTTCAAGGTGCTCCAAGAGAGCAAGGCTGAGGTGCCACTGGACATTGTCCAGAGCACTTGTAAATACCCTGACTACCTGAAAATGAGTGCTGATGCCTATGGAGACAGTATGTGGTTCTACCTGAGGAGGGAACAACTTTTTGCCAGACACTACTTCAACAGGGCTGGCAAGGTGGGAGAGACCATCCCTGCTGAACTCTACCTGAAAGGCAGCAATGGCAGGGAACCTCCTCCATCCTCTGTCTATGTGGCTACACCATCTGGCAGTATGATTACCTCTGAGGCTCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAACTTTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCATCAGCACAGCCACAGAACAACTTAGCAAATATGATGCCAGGAAGATAAACCAATACCTGAGGCATGTGGAGGAATATGAACTCCAATTTGTGTTCCAACTTTGTAAGATTACCCTGTCTGCTGAGGTGATGGCTTACCTGCACAATATGAATGCCAACCTGTTGGAGGACTGGAACATTGGACTGAGCCCTCCTGTGGCTACCTCCTTGGAGGACAAATACAGATATGTGAGGAGCACAGCCATCACTTGTCAGAGGGAACAACCTCCAACAGAGAAGCAGGACCCACTGGCTAAATACAAGTTCTGGGATGTGAACCTCCAAGACTCCTTCAGCACAGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTG 2 CTCCAAGCAG 31: KpnⅠ酶切位點; 2: 編碼HPV56L1的1-467胺基酸的基因片段; 3: 與HPV33L1的469-499胺基酸的基因片段重疊的10個鹼基. |
SEQ ID No: 143 | 5610 HPV56L1 F2 GAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCC |
SEQ ID No: 144 | 5611 HPV56L1 R2 CTGTCTAGATTTACTTCTTCACCTTCTTCCTCTTGG |
SEQ ID No: 145 | 5612 HPV56L1擴增序列2 GAAGTTCCTG 1 CTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAG 2TAA A TCTAGA 3CAG. 1: 與HPV56L1的1-467胺基酸C端基因片段重疊的10bp鹼基; 2: HPV33L1 C端的31個(469-499)胺基酸的基因片段; 3: XbaI酶切位點. |
SEQ ID No: 146 | 5613 HPV 59型L1蛋白的471-508胺基酸序列 LQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
附錄13:序列表-嵌合的人乳頭瘤病毒58型L1蛋白
SEQ ID No: 147 | 5801 HPV 58型L1蛋白的1-473胺基酸序列 MSVWRPSEATVYLPPVPVSKVVSTDEYVSRTSIYYYAGSSRLLAVGNPYFSIKSPNNNKKVLVPKVSGLQYRVFRVRLPDPNKFGFPDTSFYNPDTQRLVWACVGLEIGRGQPLGVGVSGHPYLNKFDDTETSNRYPAQPGSDNRECLSMDYKQTQLCLIGCKPPTGEHWGKGVACNNNAAATDCPPLELFNSIIEDGDMVDTGFGCMDFGTLQANKSDVPIDICNSTCKYPDYLKMASEPYGDSLFFFLRREQMFVRHFFNRAGKLGEAVPDDLYIKGSGNTAVIQSSAFFPTPSGSIVTSESQLFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMTLCTEVTKEGTYKNDNFKEYVRHVEEYDLQFVFQLCKITLTAEIMTYIHTMDSNILEDWQFGLTPPPSASLQDTYRFVTSQAITCQKTAPPKEKEDPLNKYTFWEVNLKEKFSADLDQFPLGRKFLLQSGL |
SEQ ID No: 148 | 5802 HPV 33型L1蛋白的474-499胺基酸序列 KAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 149 | 5803 嵌合的HPV 58型L1蛋白的胺基酸序列 MSVWRPSEATVYLPPVPVSKVVSTDEYVSRTSIYYYAGSSRLLAVGNPYFSIKSPNNNKKVLVPKVSGLQYRVFRVRLPDPNKFGFPDTSFYNPDTQRLVWACVGLEIGRGQPLGVGVSGHPYLNKFDDTETSNRYPAQPGSDNRECLSMDYKQTQLCLIGCKPPTGEHWGKGVACNNNAAATDCPPLELFNSIIEDGDMVDTGFGCMDFGTLQANKSDVPIDICNSTCKYPDYLKMASEPYGDSLFFFLRREQMFVRHFFNRAGKLGEAVPDDLYIKGSGNTAVIQSSAFFPTPSGSIVTSESQLFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMTLCTEVTKEGTYKNDNFKEYVRHVEEYDLQFVFQLCKITLTAEIMTYIHTMDSNILEDWQFGLTPPPSASLQDTYRFVTSQAITCQKTAPPKEKEDPLNKYTFWEVNLKEKFSADLDQFPLGRKFLLQSGLKAKPKLKRAAPTSTRTSSAKRKKVKK |
SEQ ID No: 150 | 5804 嵌合的HPV 58型L1蛋白的核苷酸序列 ATGAGCGTGTGGAGGCCCAGCGAGGCCACCGTGTACCTGCCCCCCGTGCCCGTGAGCAAGGTGGTGAGCACCGACGAGTACGTGAGCAGGACCAGCATCTACTACTACGCCGGCAGCAGCAGGCTGCTGGCCGTGGGCAACCCCTACTTCAGCATCAAGAGCCCCAACAACAACAAGAAGGTGCTGGTGCCCAAGGTGAGCGGCCTGCAGTACAGGGTGTTCAGGGTGAGGCTGCCCGACCCCAACAAGTTCGGCTTCCCCGACACCAGCTTCTACAACCCCGACACCCAGAGGCTGGTGTGGGCCTGCGTGGGCCTGGAGATCGGCAGGGGCCAGCCCCTGGGCGTGGGCGTGAGCGGCCACCCCTACCTGAACAAGTTCGACGACACCGAGACCAGCAACAGGTACCCCGCCCAGCCCGGCAGCGACAACAGGGAGTGCCTGAGCATGGACTACAAGCAGACCCAGCTGTGCCTGATCGGCTGCAAGCCCCCCACCGGCGAGCACTGGGGCAAGGGCGTGGCCTGCAACAACAACGCCGCCGCCACCGACTGCCCCCCCCTGGAGCTGTTCAACAGCATCATCGAGGACGGCGACATGGTGGACACCGGCTTCGGCTGCATGGACTTCGGCACCCTGCAGGCCAACAAGAGCGACGTGCCCATCGACATCTGCAACAGCACCTGCAAGTACCCCGACTACCTGAAGATGGCCAGCGAGCCCTACGGCGACAGCCTGTTCTTCTTCCTGAGGAGGGAGCAGATGTTCGTGAGGCACTTCTTCAACAGGGCCGGCAAGCTGGGCGAGGCCGTGCCCGACGACCTGTACATCAAGGGCAGCGGCAACACCGCCGTGATCCAGAGCAGCGCCTTCTTCCCCACCCCCAGCGGCAGCATCGTGACCAGCGAGAGCCAGCTGTTCAACAAGCCCTACTGGCTGCAGAGGGCCCAGGGCCACAACAACGGCATCTGCTGGGGCAACCAGCTGTTCGTGACCGTGGTGGACACCACCAGGAGCACCAACATGACCCTGTGCACCGAGGTGACCAAGGAGGGCACCTACAAGAACGACAACTTCAAGGAGTACGTGAGGCACGTGGAGGAGTACGACCTGCAGTTCGTGTTCCAGCTGTGCAAGATCACCCTGACCGCCGAGATCATGACCTACATCCACACCATGGACAGCAACATCCTGGAGGACTGGCAGTTCGGCCTGACCCCCCCCCCCAGCGCCAGCCTGCAGGACACCTACAGGTTCGTGACCAGCCAGGCCATCACCTGCCAGAAGACCGCCCCCCCCAAGGAGAAGGAGGACCCCCTGAACAAGTACACCTTCTGGGAGGTGAACCTGAAGGAGAAGTTCAGCGCCGACCTGGACCAGTTCCCCCTGGGCAGGAAGTTCCTGCTGCAGAGCGGCCTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAA |
SEQ ID No: 151 | 5805 合成的HPV58L1基因 ctgggtaccATGAGCGTGTGGAGGCCCAGCGAGGCCACCGTGTACCTGCCCCCCGTGCCCGTGAGCAAGGTGGTGAGCACCGACGAGTACGTGAGCAGGACCAGCATCTACTACTACGCCGGCAGCAGCAGGCTGCTGGCCGTGGGCAACCCCTACTTCAGCATCAAGAGCCCCAACAACAACAAGAAGGTGCTGGTGCCCAAGGTGAGCGGCCTGCAGTACAGGGTGTTCAGGGTGAGGCTGCCCGACCCCAACAAGTTCGGCTTCCCCGACACCAGCTTCTACAACCCCGACACCCAGAGGCTGGTGTGGGCCTGCGTGGGCCTGGAGATCGGCAGGGGCCAGCCCCTGGGCGTGGGCGTGAGCGGCCACCCCTACCTGAACAAGTTCGACGACACCGAGACCAGCAACAGGTACCCCGCCCAGCCCGGCAGCGACAACAGGGAGTGCCTGAGCATGGACTACAAGCAGACCCAGCTGTGCCTGATCGGCTGCAAGCCCCCCACCGGCGAGCACTGGGGCAAGGGCGTGGCCTGCAACAACAACGCCGCCGCCACCGACTGCCCCCCCCTGGAGCTGTTCAACAGCATCATCGAGGACGGCGACATGGTGGACACCGGCTTCGGCTGCATGGACTTCGGCACCCTGCAGGCCAACAAGAGCGACGTGCCCATCGACATCTGCAACAGCACCTGCAAGTACCCCGACTACCTGAAGATGGCCAGCGAGCCCTACGGCGACAGCCTGTTCTTCTTCCTGAGGAGGGAGCAGATGTTCGTGAGGCACTTCTTCAACAGGGCCGGCAAGCTGGGCGAGGCCGTGCCCGACGACCTGTACATCAAGGGCAGCGGCAACACCGCCGTGATCCAGAGCAGCGCCTTCTTCCCCACCCCCAGCGGCAGCATCGTGACCAGCGAGAGCCAGCTGTTCAACAAGCCCTACTGGCTGCAGAGGGCCCAGGGCCACAACAACGGCATCTGCTGGGGCAACCAGCTGTTCGTGACCGTGGTGGACACCACCAGGAGCACCAACATGACCCTGTGCACCGAGGTGACCAAGGAGGGCACCTACAAGAACGACAACTTCAAGGAGTACGTGAGGCACGTGGAGGAGTACGACCTGCAGTTCGTGTTCCAGCTGTGCAAGATCACCCTGACCGCCGAGATCATGACCTACATCCACACCATGGACAGCAACATCCTGGAGGACTGGCAGTTCGGCCTGACCCCCCCCCCCAGCGCCAGCCTGCAGGACACCTACAGGTTCGTGACCAGCCAGGCCATCACCTGCCAGAAGACCGCCCCCCCCAAGGAGAAGGAGGACCCCCTGAACAAGTACACCTTCTGGGAGGTGAACCTGAAGGAGAAGTTCAGCGCCGACCTGGACCAGTTCCCCCTGGGCAGGAAGTTCCTGCTGCAGAGCGGCCTGAAGGCCAAGCCCAGGCTGAAGAGGAGCGCCCCCACCACCAGGGCCCCCAGCACCAAGAGGAAGAAGGTGAAGAAGTAAA ctcgagctc |
SEQ ID No: 152 | 5806 合成的HPV33L1基因 ctgggtaccATGAGTGTGTGGAGACCATCTGAGGCTACAGTCTACCTGCCTCCTGTGCCTGTGAGCAAGGTGGTGAGCACAGATGAATATGTGAGCAGGACCAGCATCTACTACTATGCTGGCTCCAGCAGACTGCTGGCTGTGGGACACCCATACTTCAGCATCAAGAACCCAACCAATGCCAAGAAACTGCTGGTGCCAAAGGTGTCTGGACTCCAATACAGGGTGTTCAGGGTGAGACTGCCTGACCCAAACAAGTTTGGCTTTCCTGACACCTCCTTCTACAACCCTGACACCCAGAGACTGGTGTGGGCTTGTGTGGGATTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGCATCTCTGGACACCCACTGCTGAACAAGTTTGATGACACAGAGACAGGCAACAAATACCCTGGACAACCTGGAGCAGACAACAGGGAGTGTCTGAGTATGGACTACAAGCAGACCCAACTTTGTCTGCTGGGCTGTAAGCCTCCAACAGGAGAACACTGGGGCAAGGGAGTGGCTTGTACCAATGCTGCCCCTGCCAATGACTGTCCTCCATTGGAACTGATAAACACCATCATTGAGGATGGAGATATGGTGGACACAGGCTTTGGCTGTATGGACTTCAAGACCCTCCAAGCCAACAAGTCTGATGTGCCAATTGACATCTGTGGCAGCACTTGTAAATACCCTGACTACCTGAAAATGACCTCTGAACCATATGGAGACTCCCTGTTCTTCTTCCTGAGGAGGGAACAGATGTTTGTGAGACACTTCTTCAACAGGGCTGGCACCCTGGGAGAGGCTGTGCCTGATGACCTCTACATCAAGGGCTCTGGCACCACAGCCAGCATCCAGTCCTCTGCCTTCTTTCCAACACCATCTGGCAGTATGGTGACCTCTGAGAGCCAACTTTTCAACAAGCCATACTGGCTCCAAAGGGCTCAAGGACACAACAATGGCATCTGTTGGGGCAACCAGGTGTTTGTGACAGTGGTGGACACCACCAGGAGCACCAATATGACCCTGTGTACCCAGGTGACCTCTGACAGCACCTACAAGAATGAGAACTTCAAGGAATACATCAGGCATGTGGAGGAATATGACCTCCAATTTGTGTTCCAACTTTGTAAGGTGACCCTGACAGCAGAGGTGATGACCTACATCCATGCTATGAACCCTGACATCTTGGAGGACTGGCAGTTTGGACTGACACCTCCTCCATCTGCCTCCCTCCAAGACACCTACAGGTTTGTGACCAGCCAGGCTATCACTTGTCAGAAGACAGTGCCTCCAAAGGAGAAGGAGGACCCACTGGGCAAATACACCTTCTGGGAGGTGGACCTGAAAGAGAAGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAAGCAGGACTGAAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGGACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAGTAAActcgagctc |
SEQ ID No: 153 | 5807 HPV58L1 F1 CTTGGTACCATGAGCGTGTGGAGGCCCAGCGAGG |
SEQ ID No: 154 | 5808 HPV58L1 R1 GCTTGGCTTTCAGGCCGCTCTGCAGCAGGAACTTCC |
SEQ ID No: 155 | 5809 HPV58L1擴增序列1 CTT GGTACC 1 ATGAGCGTGTGGAGGCCCAGCGAGGCCACCGTGTACCTGCCCCCCGTGCCCGTGAGCAAGGTGGTGAGCACCGACGAGTACGTGAGCAGGACCAGCATCTACTACTACGCCGGCAGCAGCAGGCTGCTGGCCGTGGGCAACCCCTACTTCAGCATCAAGAGCCCCAACAACAACAAGAAGGTGCTGGTGCCCAAGGTGAGCGGCCTGCAGTACAGGGTGTTCAGGGTGAGGCTGCCCGACCCCAACAAGTTCGGCTTCCCCGACACCAGCTTCTACAACCCCGACACCCAGAGGCTGGTGTGGGCCTGCGTGGGCCTGGAGATCGGCAGGGGCCAGCCCCTGGGCGTGGGCGTGAGCGGCCACCCCTACCTGAACAAGTTCGACGACACCGAGACCAGCAACAGGTACCCCGCCCAGCCCGGCAGCGACAACAGGGAGTGCCTGAGCATGGACTACAAGCAGACCCAGCTGTGCCTGATCGGCTGCAAGCCCCCCACCGGCGAGCACTGGGGCAAGGGCGTGGCCTGCAACAACAACGCCGCCGCCACCGACTGCCCCCCCCTGGAGCTGTTCAACAGCATCATCGAGGACGGCGACATGGTGGACACCGGCTTCGGCTGCATGGACTTCGGCACCCTGCAGGCCAACAAGAGCGACGTGCCCATCGACATCTGCAACAGCACCTGCAAGTACCCCGACTACCTGAAGATGGCCAGCGAGCCCTACGGCGACAGCCTGTTCTTCTTCCTGAGGAGGGAGCAGATGTTCGTGAGGCACTTCTTCAACAGGGCCGGCAAGCTGGGCGAGGCCGTGCCCGACGACCTGTACATCAAGGGCAGCGGCAACACCGCCGTGATCCAGAGCAGCGCCTTCTTCCCCACCCCCAGCGGCAGCATCGTGACCAGCGAGAGCCAGCTGTTCAACAAGCCCTACTGGCTGCAGAGGGCCCAGGGCCACAACAACGGCATCTGCTGGGGCAACCAGCTGTTCGTGACCGTGG TGGACACCACCAGGAGCACCAACATGACCCTGTGCACCGAGGTGACCAAGGAGGGCACCTACAAGAACGACAACTTCAAGGAGTACGTGAGGCACGTGGAGGAGTACGACCTGCAGTTCGTGTTCCAGCTGTGCAAGATCACCCTGACCGCCGAGATCATGACCTACATCCACACCATGGACAGCAACATCCTGGAGGACTGGCAGTTCGGCCTGACCCCCCCCCCCAGCGCCAGCCTGCAGGACACCTACAGGTTCGTGACCAGCCAGGCCATCACCTGCCAGAAGACCGCCCCCCCCAAGGAGAAGGAGGACCCCCTGAACAAGTACACCTTCTGGGAGGTGAACCTGAAGGAGAAGTTCAGCGCCGACCTGGACCAGTTCCCCCTGGGCAGGAAGTTCCTGCTGCAGAGCGGCCTG 2 AAAGCCAAGC 31: KpnⅠ酶切位點; 2: 編碼HPV58L1的1-473胺基酸的基因片段; 3: 與HPV33L1的474-499胺基酸的基因片段重疊的10個鹼基。 |
SEQ ID No: 156 | 5810 HPV58L1 F2 GAGCGGCCTGAAAGCCAAGCCAAAACTGAAAAGGG |
SEQ ID No: 157 | 5811 HPV58L1 R2 CTGTCTAGATTTACTTCTTCACCTTCTTCCTCTTGG |
SEQ ID No: 158 | 5812 HPV58L1擴增序列2 GAGCGGCCTG 1 AAAGCCAAGCCAAAACTGAAAAGGGCTGCCCCAACCAGCACCAGG ACCTCCTCTGCCAAGAGGAAGAAGGTGAAGAAG 2TAA A TCTAGA 3CAG. 1: 與HPV58L1的1-473胺基酸C端基因片段重疊的10bp鹼基; 2: HPV33L1 C端的26個(474-499)胺基酸的基因片段; 3: XbaI酶切位點 |
SEQ ID No: 159 | 5813 HPV 59型L1蛋白的471-508胺基酸序列 LQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
附錄14:序列表-人乳頭瘤病毒59型L1蛋白
SEQ ID No: 160 | 5901 HPV 59型L1蛋白的胺基酸序列 MALWRSSDNKVYLPPPSVAKVVSTDEYVTRTSIFYHAGSSRLLTVGHPYFKVPKGGNGRQDVPKVSAYQYRVFRVKLPDPNKFGLPDNTVYDPNSQRLVWACVGVEIGRGQPLGVGLSGHPLYNKLDDTENSHVASAVDTKDTRDNVSVDYKQTQLCIIGCVPAIGEHWTKGTACKPTTVVQGDCPPLELINTPIEDGDMVDTGYGAMDFKLLQDNKSEVPLDICQSICKYPDYLQMSADAYGDSMFFCLRREQVFARHFWNRSGTMGDQLPESLYIKGTDIRANPGSYLYSPSPSGSVVTSDSQLFNKPYWLHKAQGLNNGICWHNQLFLTVVDTTRSTNLSVCASTTSSIPNVYTPTSFKEYARHVEEFDLQFIFQLCKITLTTEVMSYIHNMNTTILEDWNFGVTPPPTASLVDTYRFVQSAAVTCQKDTAPPVKQDPYDKLKFWPVDLKERFSADLDQFPLGRKFLLQLGARPKPTIGPRKRAAPAPTSTPSPKRVKRRKSSRK |
SEQ ID No: 161 | 5902 HPV 59型L1蛋白的核苷酸序列 ATGGCTCTGTGGAGGTCCTCTGACAACAAGGTCTACCTGCCTCCTCCATCTGTGGCTAAGGTGGTGAGCACAGATGAATATGTGACCAGGACCAGCATCTTCTACCATGCTGGCTCCAGCAGACTGCTGACAGTGGGACACCCATACTTCAAGGTGCCAAAGGGAGGCAATGGCAGACAGGATGTGCCAAAGGTGTCTGCCTACCAATACAGGGTGTTCAGGGTGAAACTGCCTGACCCAAACAAGTTTGGACTGCCTGACAACACAGTCTATGACCCAAACAGCCAGAGACTGGTGTGGGCTTGTGTGGGAGTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGACTGTCTGGACACCCACTCTACAACAAACTGGATGACACAGAGAACTCTCATGTGGCATCTGCTGTGGACACCAAGGACACCAGGGACAATGTGTCTGTGGACTACAAGCAGACCCAACTTTGTATCATTGGCTGTGTGCCTGCCATTGGAGAACACTGGACCAAGGGCACAGCCTGTAAGCCAACCACAGTGGTCCAGGGAGACTGTCCTCCATTGGAACTGATAAACACACCAATTGAGGATGGAGATATGGTGGACACAGGCTATGGAGCTATGGACTTCAAACTGCTCCAAGACAACAAGTCTGAGGTGCCACTGGACATCTGTCAGAGCATCTGTAAATACCCTGACTACCTCCAAATGAGTGCTGATGCCTATGGAGACAGTATGTTCTTCTGTCTGAGGAGGGAACAGGTGTTTGCCAGACACTTCTGGAACAGGTCTGGCACAATGGGAGACCAACTTCCTGAGTCCCTCTACATCAAGGGCACAGACATCAGGGCTAACCCTGGCTCCTACCTCTACAGCCCAAGCCCATCTGGCTCTGTGGTGACCTCTGACAGCCAACTTTTCAACAAGCCATACTGGCTGCACAAGGCTCAAGGACTGAACAATGGCATCTGTTGGCACAACCAACTTTTCCTGACAGTGGTGGACACCACCAGGAGCACCAACCTGTCTGTGTGTGCCAGCACCACCTCCAGCATCCCAAATGTCTACACACCAACCTCCTTCAAGGAATATGCCAGGCATGTGGAGGAGTTTGACCTCCAATTCATCTTCCAACTTTGTAAGATTACCCTGACCACAGAGGTGATGAGTTACATCCACAATATGAACACCACCATCTTGGAGGACTGGAACTTTGGAGTGACACCTCCTCCAACAGCCTCCCTGGTGGACACCTACAGGTTTGTCCAGTCTGCTGCTGTGACTTGTCAGAAGGACACAGCCCCTCCTGTGAAGCAGGACCCATATGACAAACTGAAGTTCTGGCCTGTGGACCTGAAAGAGAGGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAACTTGGAGCCAGACCAAAGCCAACCATTGGACCAAGGAAGAGGGCTGCCCCTGCCCCAACCAGCACACCAAGCCCAAAGAGGGTGAAGAGGAGGAAGTCCAGCAGGAAGTAAA |
SEQ ID No: 162 | 5903 合成的HPV59L1基因 ctgggtaccATGGCTCTGTGGAGGTCCTCTGACAACAAGGTCTACCTGCCTCCTCCATCTGTGGCTAAGGTGGTGAGCACAGATGAATATGTGACCAGGACCAGCATCTTCTACCATGCTGGCTCCAGCAGACTGCTGACAGTGGGACACCCATACTTCAAGGTGCCAAAGGGAGGCAATGGCAGACAGGATGTGCCAAAGGTGTCTGCCTACCAATACAGGGTGTTCAGGGTGAAACTGCCTGACCCAAACAAGTTTGGACTGCCTGACAACACAGTCTATGACCCAAACAGCCAGAGACTGGTGTGGGCTTGTGTGGGAGTGGAGATTGGCAGGGGACAACCACTGGGAGTGGGACTGTCTGGACACCCACTCTACAACAAACTGGATGACACAGAGAACTCTCATGTGGCATCTGCTGTGGACACCAAGGACACCAGGGACAATGTGTCTGTGGACTACAAGCAGACCCAACTTTGTATCATTGGCTGTGTGCCTGCCATTGGAGAACACTGGACCAAGGGCACAGCCTGTAAGCCAACCACAGTGGTCCAGGGAGACTGTCCTCCATTGGAACTGATAAACACACCAATTGAGGATGGAGATATGGTGGACACAGGCTATGGAGCTATGGACTTCAAACTGCTCCAAGACAACAAGTCTGAGGTGCCACTGGACATCTGTCAGAGCATCTGTAAATACCCTGACTACCTCCAAATGAGTGCTGATGCCTATGGAGACAGTATGTTCTTCTGTCTGAGGAGGGAACAGGTGTTTGCCAGACACTTCTGGAACAGGTCTGGCACAATGGGAGACCAACTTCCTGAGTCCCTCTACATCAAGGGCACAGACATCAGGGCTAACCCTGGCTCCTACCTCTACAGCCCAAGCCCATCTGGCTCTGTGGTGACCTCTGACAGCCAACTTTTCAACAAGCCATACTGGCTGCACAAGGCTCAAGGACTGAACAATGGCATCTGTTGGCACAACCAACTTTTCCTGACAGTGGTGGACACCACCAGGAGCACCAACCTGTCTGTGTGTGCCAGCACCACCTCCAGCATCCCAAATGTCTACACACCAACCTCCTTCAAGGAATATGCCAGGCATGTGGAGGAGTTTGACCTCCAATTCATCTTCCAACTTTGTAAGATTACCCTGACCACAGAGGTGATGAGTTACATCCACAATATGAACACCACCATCTTGGAGGACTGGAACTTTGGAGTGACACCTCCTCCAACAGCCTCCCTGGTGGACACCTACAGGTTTGTCCAGTCTGCTGCTGTGACTTGTCAGAAGGACACAGCCCCTCCTGTGAAGCAGGACCCATATGACAAACTGAAGTTCTGGCCTGTGGACCTGAAAGAGAGGTTCTCTGCTGACCTGGACCAGTTTCCACTGGGCAGGAAGTTCCTGCTCCAACTTGGAGCCAGACCAAAGCCAACCATTGGACCAAGGAAGAGGGCTGCCCCTGCCCCAACCAGCACACCAAGCCCAAAGAGGGTGAAGAGGAGGAAGTCCAGCAGGAAGTAAActcgagctc |
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圖1A HPV 6 L1:33C的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1B HPV 11 L1:33C的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1C HPV 16 L1:33C的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1D HPV 18 L1:33C的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1E HPV 31 L1:33C的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1F HPV 33 L1的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1G HPV 35 L1:33C的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1H HPV 39 L1:59C的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1I HPV 45 L1:33C的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1J HPV 51 L1:33C的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1K HPV 52 L1:33C的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1L HPV 56 L1:33C的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1M HPV 58 L1:33C的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖1N HPV 59 L1的L1蛋白的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
圖2A 穿透式電子顯微鏡觀察HPV 6 L1:33C類病毒顆粒。
圖2B 穿透式電子顯微鏡觀察HPV 11 L1:33C類病毒顆粒。
圖2C 穿透式電子顯微鏡觀察HPV 16 L1:33C類病毒顆粒。
圖2D 穿透式電子顯微鏡觀察HPV 18 L1:33C類病毒顆粒。
圖2E 穿透式電子顯微鏡觀察HPV 31 L1:33C類病毒顆粒。
圖2F 穿透式電子顯微鏡觀察HPV 33 L1類病毒顆粒。
圖2G 穿透式電子顯微鏡觀察HPV 35 L1:33C類病毒顆粒。
圖2H 穿透式電子顯微鏡觀察HPV 39 L1:59C類病毒顆粒。
圖2I 穿透式電子顯微鏡觀察HPV 45 L1:33C類病毒顆粒。
圖2J 穿透式電子顯微鏡觀察HPV 51 L1:33C類病毒顆粒。
圖2K 穿透式電子顯微鏡觀察HPV 52 L1:33C類病毒顆粒。
圖2L 穿透式電子顯微鏡觀察HPV 56 L1:33C類病毒顆粒。
圖2M 穿透式電子顯微鏡觀察HPV 58 L1:33C類病毒顆粒。
圖2N 穿透式電子顯微鏡觀察HPV 59 L1類病毒顆粒。
圖3 14型類病毒顆粒組合物免疫小鼠後的假病毒中和效價。動物數,N=10。GMT(Geometric Mean Titer):幾何平均效價。
圖4 C端截短的HPV16L1(1-474)的表達。M:Marker;L:細胞裂解液;E-S:裂解液離心後收集的上清液。
無
序列表 <![CDATA[<110> 大陸商神州細胞工程有限公司]]> <![CDATA[<120> 人乳頭瘤病毒多價免疫原性組合物]]> <![CDATA[<140> TW109135395]]> <![CDATA[<141> 2020-10-13]]> <![CDATA[<160> 162]]> <![CDATA[<170> BiSSAP 1.3.6]]> <![CDATA[<210> 1]]> <![CDATA[<211> 469]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 0601 HPV 6型L1蛋白的1-469胺基酸序列]]> <![CDATA[<400> 1]]> Met Trp Arg Pro Ser Asp Ser Thr Val Tyr Val Pro Pro Pro Asn Pro 1 5 10 15 Val Ser Lys Val Val Ala Thr Asp Ala Tyr Val Thr Arg Thr Asn Ile 20 25 30 Phe Tyr His Ala Ser Ser Ser Arg Leu Leu Ala Val Gly His Pro Tyr 35 40 45 Phe Ser Ile Lys Arg Ala Asn Lys Thr Val Val Pro Lys Val Ser Gly 50 55 60 Tyr Gln Tyr Arg Val Phe Lys Val Val Leu Pro Asp Pro Asn Lys Phe 65 70 75 80 Ala Leu Pro Asp Ser Ser Leu Phe Asp Pro Thr Thr Gln Arg Leu Val 85 90 95 Trp Ala Cys Thr Gly Leu Glu Val Gly Arg Gly Gln Pro Leu Gly Val 100 105 110 Gly Val Ser Gly His Pro Phe Leu Asn Lys Tyr Asp Asp Val Glu Asn 115 120 125 Ser Gly Ser Gly Gly Asn Pro Gly Gln Asp Asn Arg Val Asn Val Gly 130 135 140 Met Asp Tyr Lys Gln Thr Gln Leu Cys Met Val Gly Cys Ala Pro Pro 145 150 155 160 Leu Gly Glu His Trp Gly Lys Gly Lys Gln Cys Thr Asn Thr Pro Val 165 170 175 Gln Ala Gly Asp Cys Pro Pro Leu Glu Leu Ile Thr Ser Val Ile Gln 180 185 190 Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala Met Asn Phe Ala Asp 195 200 205 Leu Gln Thr Asn Lys Ser Asp Val Pro Ile Asp Ile Cys Gly Thr Thr 210 215 220 Cys Lys Tyr Pro Asp Tyr Leu Gln Met Ala Ala Asp Pro Tyr Gly Asp 225 230 235 240 Arg Leu Phe Phe Phe Leu Arg Lys Glu Gln Met Phe Ala Arg His Phe 245 250 255 Phe Asn Arg Ala Gly Glu Val Gly Glu Pro Val Pro Asp Thr Leu Ile 260 265 270 Ile Lys Gly Ser Gly Asn Arg Thr Ser Val Gly Ser Ser Ile Tyr Val 275 280 285 Asn Thr Pro Ser Gly Ser Leu Val Ser Ser Glu Ala Gln Leu Phe Asn 290 295 300 Lys Pro Tyr Trp Leu Gln Lys Ala Gln Gly His Asn Asn Gly Ile Cys 305 310 315 320 Trp Gly Asn Gln Leu Phe Val Thr Val Val Asp Thr Thr Arg Ser Thr 325 330 335 Asn Met Thr Leu Cys Ala Ser Val Thr Thr Ser Ser Thr Tyr Thr Asn 340 345 350 Ser Asp Tyr Lys Glu Tyr Met Arg His Val Glu Glu Tyr Asp Leu Gln 355 360 365 Phe Ile Phe Gln Leu Cys Ser Ile Thr Leu Ser Ala Glu Val Met Ala 370 375 380 Tyr Ile His Thr Met Asn Pro Ser Val Leu Glu Asp Trp Asn Phe Gly 385 390 395 400 Leu Ser Pro Pro Pro Asn Gly Thr Leu Glu Asp Thr Tyr Arg Tyr Val 405 410 415 Gln Ser Gln Ala Ile Thr Cys Gln Lys Pro Thr Pro Glu Lys Glu Lys 420 425 430 Pro Asp Pro Tyr Lys Asn Leu Ser Phe Trp Glu Val Asn Leu Lys Glu 435 440 445 Lys Phe Ser Ser Glu Leu Asp Gln Tyr Pro Leu Gly Arg Lys Phe Leu 450 455 460 Leu Gln Ser Gly Tyr 465 <![CDATA[<210> 2]]> <![CDATA[<211> 26]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 0602 HPV 33型L1蛋白的474-499胺基酸序列]]> <![CDATA[<400> 2]]> Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr 1 5 10 15 Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 20 25 <![CDATA[<210> 3]]> <![CDATA[<211> 495]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 0603 嵌合的HPV 6型L1蛋白的胺基酸序列]]> <![CDATA[<400> 3]]> Met Trp Arg Pro Ser Asp Ser Thr Val Tyr Val Pro Pro Pro Asn Pro 1 5 10 15 Val Ser Lys Val Val Ala Thr Asp Ala Tyr Val Thr Arg Thr Asn Ile 20 25 30 Phe Tyr His Ala Ser Ser Ser Arg Leu Leu Ala Val Gly His Pro Tyr 35 40 45 Phe Ser Ile Lys Arg Ala Asn Lys Thr Val Val Pro Lys Val Ser Gly 50 55 60 Tyr Gln Tyr Arg Val Phe Lys Val Val Leu Pro Asp Pro Asn Lys Phe 65 70 75 80 Ala Leu Pro Asp Ser Ser Leu Phe Asp Pro Thr Thr Gln Arg Leu Val 85 90 95 Trp Ala Cys Thr Gly Leu Glu Val Gly Arg Gly Gln Pro Leu Gly Val 100 105 110 Gly Val Ser Gly His Pro Phe Leu Asn Lys Tyr Asp Asp Val Glu Asn 115 120 125 Ser Gly Ser Gly Gly Asn Pro Gly Gln Asp Asn Arg Val Asn Val Gly 130 135 140 Met Asp Tyr Lys Gln Thr Gln Leu Cys Met Val Gly Cys Ala Pro Pro 145 150 155 160 Leu Gly Glu His Trp Gly Lys Gly Lys Gln Cys Thr Asn Thr Pro Val 165 170 175 Gln Ala Gly Asp Cys Pro Pro Leu Glu Leu Ile Thr Ser Val Ile Gln 180 185 190 Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala Met Asn Phe Ala Asp 195 200 205 Leu Gln Thr Asn Lys Ser Asp Val Pro Ile Asp Ile Cys Gly Thr Thr 210 215 220 Cys Lys Tyr Pro Asp Tyr Leu Gln Met Ala Ala Asp Pro Tyr Gly Asp 225 230 235 240 Arg Leu Phe Phe Phe Leu Arg Lys Glu Gln Met Phe Ala Arg His Phe 245 250 255 Phe Asn Arg Ala Gly Glu Val Gly Glu Pro Val Pro Asp Thr Leu Ile 260 265 270 Ile Lys Gly Ser Gly Asn Arg Thr Ser Val Gly Ser Ser Ile Tyr Val 275 280 285 Asn Thr Pro Ser Gly Ser Leu Val Ser Ser Glu Ala Gln Leu Phe Asn 290 295 300 Lys Pro Tyr Trp Leu Gln Lys Ala Gln Gly His Asn Asn Gly Ile Cys 305 310 315 320 Trp Gly Asn Gln Leu Phe Val Thr Val Val Asp Thr Thr Arg Ser Thr 325 330 335 Asn Met Thr Leu Cys Ala Ser Val Thr Thr Ser Ser Thr Tyr Thr Asn 340 345 350 Ser Asp Tyr Lys Glu Tyr Met Arg His Val Glu Glu Tyr Asp Leu Gln 355 360 365 Phe Ile Phe Gln Leu Cys Ser Ile Thr Leu Ser Ala Glu Val Met Ala 370 375 380 Tyr Ile His Thr Met Asn Pro Ser Val Leu Glu Asp Trp Asn Phe Gly 385 390 395 400 Leu Ser Pro Pro Pro Asn Gly Thr Leu Glu Asp Thr Tyr Arg Tyr Val 405 410 415 Gln Ser Gln Ala Ile Thr Cys Gln Lys Pro Thr Pro Glu Lys Glu Lys 420 425 430 Pro Asp Pro Tyr Lys Asn Leu Ser Phe Trp Glu Val Asn Leu Lys Glu 435 440 445 Lys Phe Ser Ser Glu Leu Asp Gln Tyr Pro Leu Gly Arg Lys Phe Leu 450 455 460 Leu Gln Ser Gly Tyr Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro 465 470 475 480 Thr Ser Thr Arg Thr Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 485 490 495 <![CDATA[<210> 4]]> <![CDATA[<211> 1488]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 0604 嵌合的HPV 6型L1蛋白的核苷酸序列]]> <![CDATA[<400> 4]]> atgtggagac catctgacag cacagtctat gtgcctcctc caaaccctgt gagcaaggtg 60 gtggctacag atgcctatgt gaccaggacc aacatcttct accatgcctc ctccagcaga 120 ctgctggctg tgggacaccc atacttcagc atcaagaggg ctaacaagac agtggtgcca 180 aaggtgtctg gctaccaata cagggtgttc aaggtggtgc tgcctgaccc aaacaagttt 240 gccctgcctg actcctccct gtttgaccca accacccaga gactggtgtg ggcttgtact 300 ggattggagg tgggcagggg acaaccactg ggagtgggag tgtctggaca cccattcctg 360 aacaaatatg atgatgtgga gaactctggc tctggaggca accctggaca agacaacagg 420 gtgaatgtgg ggatggacta caagcagacc caactttgta tggtgggctg tgcccctcca 480 ctgggagaac actggggcaa gggcaagcag tgtaccaaca cacctgtcca ggctggagac 540 tgtcctccat tggaactgat tacctctgtg attcaggatg gagatatggt ggacacaggc 600 tttggagcta tgaactttgc tgacctccaa accaacaagt ctgatgtgcc aattgacatc 660 tgtggcacca cttgtaaata ccctgactac ctccaaatgg ctgctgaccc atatggagac 720 agactgttct tcttcctgag gaaggaacag atgtttgcca gacacttctt caacagggct 780 ggagaggtgg gagaacctgt gcctgacacc ctgattatca agggctctgg caacaggacc 840 tctgtgggct ccagcatcta tgtgaacaca ccatctggct ccctggtgtc ctctgaggct 900 caacttttca acaagccata ctggctccaa aaggctcaag gacacaacaa tggcatctgt 960 tggggcaacc aactttttgt gacagtggtg gacaccacca ggagcaccaa tatgaccctg 1020 tgtgcctctg tgaccacctc cagcacctac accaactctg actacaagga atatatgagg 1080 catgtggagg aatatgacct ccaattcatc ttccaacttt gtagcatcac cctgtctgct 1140 gaggtgatgg cttacatcca cacaatgaac ccatctgtgt tggaggactg gaactttgga 1200 ctgagccctc ctccaaatgg caccttggag gacacctaca gatatgtcca gagccaggct 1260 atcacttgtc agaagccaac acctgagaag gagaagcctg acccatacaa gaacctgtcc 1320 ttctgggagg tgaacctgaa agagaagttc tcctctgaac tggaccaata cccactgggc 1380 aggaagttcc tgctccaatc tggctacaaa gccaagccaa aactgaaaag ggctgcccca 1440 accagcacca ggacctcctc tgccaagagg aagaaggtga agaagtaa 1488 <![CDATA[<210> 5]]> <![CDATA[<211> 1522]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 0605 合成的HPV6L1基因]]> <![CDATA[<400> 5]]> ctgggtacca tgtggagacc atctgacagc acagtctatg tgcctcctcc aaaccctgtg 60 agcaaggtgg tggctacaga tgcctatgtg accaggacca acatcttcta ccatgcctcc 120 tccagcagac tgctggctgt gggacaccca tacttcagca tcaagagggc taacaagaca 180 gtggtgccaa aggtgtctgg ctaccaatac agggtgttca aggtggtgct gcctgaccca 240 aacaagtttg ccctgcctga ctcctccctg tttgacccaa ccacccagag actggtgtgg 300 gcttgtactg gattggaggt gggcagggga caaccactgg gagtgggagt gtctggacac 360 ccattcctga acaaatatga tgatgtggag aactctggct ctggaggcaa ccctggacaa 420 gacaacaggg tgaatgtggg gatggactac aagcagaccc aactttgtat ggtgggctgt 480 gcccctccac tgggagaaca ctggggcaag ggcaagcagt gtaccaacac acctgtccag 540 gctggagact gtcctccatt ggaactgatt acctctgtga ttcaggatgg agatatggtg 600 gacacaggct ttggagctat gaactttgct gacctccaaa ccaacaagtc tgatgtgcca 660 attgacatct gtggcaccac ttgtaaatac cctgactacc tccaaatggc tgctgaccca 720 tatggagaca gactgttctt cttcctgagg aaggaacaga tgtttgccag acacttcttc 780 aacagggctg gagaggtggg agaacctgtg cctgacaccc tgattatcaa gggctctggc 840 aacaggacct ctgtgggctc cagcatctat gtgaacacac catctggctc cctggtgtcc 900 tctgaggctc aacttttcaa caagccatac tggctccaaa aggctcaagg acacaacaat 960 ggcatctgtt ggggcaacca actttttgtg acagtggtgg acaccaccag gagcaccaat 1020 atgaccctgt gtgcctctgt gaccacctcc agcacctaca ccaactctga ctacaaggaa 1080 tatatgaggc atgtggagga atatgacctc caattcatct tccaactttg tagcatcacc 1140 ctgtctgctg aggtgatggc ttacatccac acaatgaacc catctgtgtt ggaggactgg 1200 aactttggac tgagccctcc tccaaatggc accttggagg acacctacag atatgtccag 1260 agccaggcta tcacttgtca gaagccaaca cctgagaagg agaagcctga cccatacaag 1320 aacctgtcct tctgggaggt gaacctgaaa gagaagttct cctctgaact ggaccaatac 1380 ccactgggca ggaagttcct gctccaatct ggctacaggg gcaggtccag catcaggaca 1440 ggagtgaaga gacctgctgt gagcaaggca tctgctgccc caaagaggaa gagggctaag 1500 accaagaggt aaactcgagc tc 1522 <![CDATA[<210> 6]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<22]]>3> 0606 合成的HPV33L1基因]]> <br/> <br/><![CDATA[<400> 6]]> <br/><![CDATA[ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct 120 ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat 180 gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga 240 ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag 300 agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc 360 atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct 420 ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt 480 tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc 540 aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat 600 ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag 660 tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg 720 acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg 780 agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc 840 aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc 900 agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc 1020 aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag 1080 aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt 1140 tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc 1200 ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac 1260 aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag 1320 gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac 1380 ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca 1440 aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg 1500 aagaagtaaa ctcgagctc 1519 <![CDATA[<210> 7]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 0607 HPV6L1 F1]]> <![CDATA[<400> 7]]> cttggtacca tgtggagacc atctgacagc acagt 35 <![CDATA[<210> 8]]> <![CDATA[<211> 36]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 0608 HPV6L1 R1]]> <![CDATA[<400> 8]]> gcttggcttt gtagccagat tggagcagga acttcc 36 <![CDATA[<210> 9]]> <![CDATA[<211> 1426]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 0609 HPV6L1擴增序列1]]> <![CDATA[<400> 9]]> cttggtacca tgtggagacc atctgacagc acagtctatg tgcctcctcc aaaccctgtg 60 agcaaggtgg tggctacaga tgcctatgtg accaggacca acatcttcta ccatgcctcc 120 tccagcagac tgctggctgt gggacaccca tacttcagca tcaagagggc taacaagaca 180 gtggtgccaa aggtgtctgg ctaccaatac agggtgttca aggtggtgct gcctgaccca 240 aacaagtttg ccctgcctga ctcctccctg tttgacccaa ccacccagag actggtgtgg 300 gcttgtactg gattggaggt gggcagggga caaccactgg gagtgggagt gtctggacac 360 ccattcctga acaaatatga tgatgtggag aactctggct ctggaggcaa ccctggacaa 420 gacaacaggg tgaatgtggg gatggactac aagcagaccc aactttgtat ggtgggctgt 480 gcccctccac tgggagaaca ctggggcaag ggcaagcagt gtaccaacac acctgtccag 540 gctggagact gtcctccatt ggaactgatt acctctgtga ttcaggatgg agatatggtg 600 gacacaggct ttggagctat gaactttgct gacctccaaa ccaacaagtc tgatgtgcca 660 attgacatct gtggcaccac ttgtaaatac cctgactacc tccaaatggc tgctgaccca 720 tatggagaca gactgttctt cttcctgagg aaggaacaga tgtttgccag acacttcttc 780 aacagggctg gagaggtggg agaacctgtg cctgacaccc tgattatcaa gggctctggc 840 aacaggacct ctgtgggctc cagcatctat gtgaacacac catctggctc cctggtgtcc 900 tctgaggctc aacttttcaa caagccatac tggctccaaa aggctcaagg acacaacaat 960 ggcatctgtt ggggcaacca actttttgtg acagtggtgg acaccaccag gagcaccaat 1020 atgaccctgt gtgcctctgt gaccacctcc agcacctaca ccaactctga ctacaaggaa 1080 tatatgaggc atgtggagga atatgacctc caattcatct tccaactttg tagcatcacc 1140 ctgtctgctg aggtgatggc ttacatccac acaatgaacc catctgtgtt ggaggactgg 1200 aactttggac tgagccctcc tccaaatggc accttggagg acacctacag atatgtccag 1260 agccaggcta tcacttgtca gaagccaaca cctgagaagg agaagcctga cccatacaag 1320 aacctgtcct tctgggaggt gaacctgaaa gagaagttct cctctgaact ggaccaatac 1380 ccactgggca ggaagttcct gctccaatct ggctacaaag ccaagc 1426 <![CDATA[<210> 10]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 0610 HPV6L1 F2]]> <![CDATA[<400> 10]]> atctggctac aaagccaagc caaaactgaa aaggg 35 <![CDATA[<210> 11]]> <![CDATA[<211> 37]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 0611 HPV6L1 R2]]> <![CDATA[<400> 11]]> ctgtctagat ttacttcttc accttcttcc tcttggc 37 <![CDATA[<210> 12]]> <![CDATA[<211> 101]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 0612 HPV6L1擴增序列2]]> <![CDATA[<400> 12]]> atctggctac aaagccaagc caaaactgaa aagggctgcc ccaaccagca ccaggacctc 60 ctctgccaag aggaagaagg tgaagaagta aatctagaca g 101 <![CDATA[<210> 13]]> <![CDATA[<211> 38]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 0613 HPV 59型L1蛋白的471-508胺基酸序列]]> <![CDATA[<400> 13]]> Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 1 5 10 15 Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 20 25 30 Arg Lys Ser Ser Arg Lys 35 <![CDATA[<210> 14]]> <![CDATA[<211> 470]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 1101 HPV 11型L1蛋白的1-470胺基酸序列]]> <![CDATA[<400> 14]]> Met Trp Arg Pro Ser Asp Ser Thr Val Tyr Val Pro Pro Pro Asn Pro 1 5 10 15 Val Ser Lys Val Val Ala Thr Asp Ala Tyr Val Lys Arg Thr Asn Ile 20 25 30 Phe Tyr His Ala Ser Ser Ser Arg Leu Leu Ala Val Gly His Pro Tyr 35 40 45 Tyr Ser Ile Lys Lys Val Asn Lys Thr Val Val Pro Lys Val Ser Gly 50 55 60 Tyr Gln Tyr Arg Val Phe Lys Val Val Leu Pro Asp Pro Asn Lys Phe 65 70 75 80 Ala Leu Pro Asp Ser Ser Leu Phe Asp Pro Thr Thr Gln Arg Leu Val 85 90 95 Trp Ala Cys Thr Gly Leu Glu Val Gly Arg Gly Gln Pro Leu Gly Val 100 105 110 Gly Val Ser Gly His Pro Leu Leu Asn Lys Tyr Asp Asp Val Glu Asn 115 120 125 Ser Gly Gly Tyr Gly Gly Asn Pro Gly Gln Asp Asn Arg Val Asn Val 130 135 140 Gly Met Asp Tyr Lys Gln Thr Gln Leu Cys Met Val Gly Cys Ala Pro 145 150 155 160 Pro Leu Gly Glu His Trp Gly Lys Gly Thr Gln Cys Ser Asn Thr Ser 165 170 175 Val Gln Asn Gly Asp Cys Pro Pro Leu Glu Leu Ile Thr Ser Val Ile 180 185 190 Gln Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala Met Asn Phe Ala 195 200 205 Asp Leu Gln Thr Asn Lys Ser Asp Val Pro Leu Asp Ile Cys Gly Thr 210 215 220 Val Cys Lys Tyr Pro Asp Tyr Leu Gln Met Ala Ala Asp Pro Tyr Gly 225 230 235 240 Asp Arg Leu Phe Phe Tyr Leu Arg Lys Glu Gln Met Phe Ala Arg His 245 250 255 Phe Phe Asn Arg Ala Gly Thr Val Gly Glu Pro Val Pro Asp Asp Leu 260 265 270 Leu Val Lys Gly Gly Asn Asn Arg Ser Ser Val Ala Ser Ser Ile Tyr 275 280 285 Val His Thr Pro Ser Gly Ser Leu Val Ser Ser Glu Ala Gln Leu Phe 290 295 300 Asn Lys Pro Tyr Trp Leu Gln Lys Ala Gln Gly His Asn Asn Gly Ile 305 310 315 320 Cys Trp Gly Asn His Leu Phe Val Thr Val Val Asp Thr Thr Arg Ser 325 330 335 Thr Asn Met Thr Leu Cys Ala Ser Val Ser Lys Ser Ala Thr Tyr Thr 340 345 350 Asn Ser Asp Tyr Lys Glu Tyr Met Arg His Val Glu Glu Phe Asp Leu 355 360 365 Gln Phe Ile Phe Gln Leu Cys Ser Ile Thr Leu Ser Ala Glu Val Met 370 375 380 Ala Tyr Ile His Thr Met Asn Pro Ser Val Leu Glu Asp Trp Asn Phe 385 390 395 400 Gly Leu Ser Pro Pro Pro Asn Gly Thr Leu Glu Asp Thr Tyr Arg Tyr 405 410 415 Val Gln Ser Gln Ala Ile Thr Cys Gln Lys Pro Thr Pro Glu Lys Glu 420 425 430 Lys Gln Asp Pro Tyr Lys Asp Met Ser Phe Trp Glu Val Asn Leu Lys 435 440 445 Glu Lys Phe Ser Ser Glu Leu Asp Gln Phe Pro Leu Gly Arg Lys Phe 450 455 460 Leu Leu Gln Ser Gly Tyr 465 470 <![CDATA[<210> 15]]> <![CDATA[<211> 26]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 1102 HPV 33型L1蛋白的474-499胺基酸序列]]> <![CDATA[<400> 15]]> Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr 1 5 10 15 Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 20 25 <![CDATA[<210> 16]]> <![CDATA[<211> 496]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1103 嵌合的HPV 11型L1蛋白的胺基酸序列]]> <![CDATA[<400> 16]]> Met Trp Arg Pro Ser Asp Ser Thr Val Tyr Val Pro Pro Pro Asn Pro 1 5 10 15 Val Ser Lys Val Val Ala Thr Asp Ala Tyr Val Lys Arg Thr Asn Ile 20 25 30 Phe Tyr His Ala Ser Ser Ser Arg Leu Leu Ala Val Gly His Pro Tyr 35 40 45 Tyr Ser Ile Lys Lys Val Asn Lys Thr Val Val Pro Lys Val Ser Gly 50 55 60 Tyr Gln Tyr Arg Val Phe Lys Val Val Leu Pro Asp Pro Asn Lys Phe 65 70 75 80 Ala Leu Pro Asp Ser Ser Leu Phe Asp Pro Thr Thr Gln Arg Leu Val 85 90 95 Trp Ala Cys Thr Gly Leu Glu Val Gly Arg Gly Gln Pro Leu Gly Val 100 105 110 Gly Val Ser Gly His Pro Leu Leu Asn Lys Tyr Asp Asp Val Glu Asn 115 120 125 Ser Gly Gly Tyr Gly Gly Asn Pro Gly Gln Asp Asn Arg Val Asn Val 130 135 140 Gly Met Asp Tyr Lys Gln Thr Gln Leu Cys Met Val Gly Cys Ala Pro 145 150 155 160 Pro Leu Gly Glu His Trp Gly Lys Gly Thr Gln Cys Ser Asn Thr Ser 165 170 175 Val Gln Asn Gly Asp Cys Pro Pro Leu Glu Leu Ile Thr Ser Val Ile 180 185 190 Gln Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala Met Asn Phe Ala 195 200 205 Asp Leu Gln Thr Asn Lys Ser Asp Val Pro Leu Asp Ile Cys Gly Thr 210 215 220 Val Cys Lys Tyr Pro Asp Tyr Leu Gln Met Ala Ala Asp Pro Tyr Gly 225 230 235 240 Asp Arg Leu Phe Phe Tyr Leu Arg Lys Glu Gln Met Phe Ala Arg His 245 250 255 Phe Phe Asn Arg Ala Gly Thr Val Gly Glu Pro Val Pro Asp Asp Leu 260 265 270 Leu Val Lys Gly Gly Asn Asn Arg Ser Ser Val Ala Ser Ser Ile Tyr 275 280 285 Val His Thr Pro Ser Gly Ser Leu Val Ser Ser Glu Ala Gln Leu Phe 290 295 300 Asn Lys Pro Tyr Trp Leu Gln Lys Ala Gln Gly His Asn Asn Gly Ile 305 310 315 320 Cys Trp Gly Asn His Leu Phe Val Thr Val Val Asp Thr Thr Arg Ser 325 330 335 Thr Asn Met Thr Leu Cys Ala Ser Val Ser Lys Ser Ala Thr Tyr Thr 340 345 350 Asn Ser Asp Tyr Lys Glu Tyr Met Arg His Val Glu Glu Phe Asp Leu 355 360 365 Gln Phe Ile Phe Gln Leu Cys Ser Ile Thr Leu Ser Ala Glu Val Met 370 375 380 Ala Tyr Ile His Thr Met Asn Pro Ser Val Leu Glu Asp Trp Asn Phe 385 390 395 400 Gly Leu Ser Pro Pro Pro Asn Gly Thr Leu Glu Asp Thr Tyr Arg Tyr 405 410 415 Val Gln Ser Gln Ala Ile Thr Cys Gln Lys Pro Thr Pro Glu Lys Glu 420 425 430 Lys Gln Asp Pro Tyr Lys Asp Met Ser Phe Trp Glu Val Asn Leu Lys 435 440 445 Glu Lys Phe Ser Ser Glu Leu Asp Gln Phe Pro Leu Gly Arg Lys Phe 450 455 460 Leu Leu Gln Ser Gly Tyr Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala 465 470 475 480 Pro Thr Ser Thr Arg Thr Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 485 490 495 <![CDATA[<210> 17]]> <![CDATA[<211> 1492]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1104 嵌合的HPV 11型L1蛋白的核苷酸序列]]> <![CDATA[<400> 17]]> atgtggagac catctgacag cacagtctat gtgcctcctc caaaccctgt gagcaaggtg 60 gtggctacag atgcctatgt gaagaggacc aacatcttct accatgcctc ctccagcaga 120 ctgctggctg tgggacaccc atactacagc atcaagaagg tgaacaagac agtggtgcca 180 aaggtgtctg gctaccaata cagggtgttc aaggtggtgc tgcctgaccc aaacaagttt 240 gccctgcctg actcctccct gtttgaccca accacccaga gactggtgtg ggcttgtact 300 ggattggagg tgggcagggg acaaccactg ggagtgggag tgtctggaca cccactgctg 360 aacaaatatg atgatgtgga gaactctgga ggctatggag gcaaccctgg acaagacaac 420 agggtgaatg tggggatgga ctacaagcag acccaacttt gtatggtggg ctgtgcccct 480 ccactgggag aacactgggg caagggcacc cagtgtagca acacctctgt ccagaatgga 540 gactgtcctc cattggaact gattacctct gtgattcagg atggagatat ggtggacaca 600 ggctttggag ctatgaactt tgctgacctc caaaccaaca agtctgatgt gccactggac 660 atctgtggca cagtgtgtaa ataccctgac tacctccaaa tggctgctga cccatatgga 720 gacagactgt tcttctacct gaggaaggaa cagatgtttg ccagacactt cttcaacagg 780 gctggcacag tgggagaacc tgtgcctgat gacctgctgg tgaagggagg caacaacagg 840 tcctctgtgg catccagcat ctatgtgcat acaccatctg gctccctggt gtcctctgag 900 gctcaacttt tcaacaagcc atactggctc caaaaggctc aaggacacaa caatggcatc 960 tgttggggca accacctgtt tgtgacagtg gtggacacca ccaggagcac caatatgacc 1020 ctgtgtgcct ctgtgagcaa gtctgccacc tacaccaact ctgactacaa ggaatatatg 1080 aggcatgtgg aggagtttga cctccaattc atcttccaac tttgtagcat caccctgtct 1140 gctgaggtga tggcttacat ccacacaatg aacccatctg tgttggagga ctggaacttt 1200 ggactgagcc ctcctccaaa tggcaccttg gaggacacct acagatatgt ccagagccag 1260 gctatcactt gtcagaagcc aacacctgag aaggagaagc aggacccata caaggatatg 1320 agtttctggg aggtgaacct gaaagagaag ttctcctctg aactggacca gtttccactg 1380 ggcaggaagt tcctgctcca atctggctac aaagccaagc caaaactgaa aagggctgcc 1440 ccaaccagca ccaggacctc ctctgccaag aggaagaagg tgaagaagta aa 1492 <![CDATA[<210> 18]]> <![CDATA[<211> 1525]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1105 合成的HPV11L1基因]]> <![CDATA[<400> 18]]> ctgggtacca tgtggagacc atctgacagc acagtctatg tgcctcctcc aaaccctgtg 60 agcaaggtgg tggctacaga tgcctatgtg aagaggacca acatcttcta ccatgcctcc 120 tccagcagac tgctggctgt gggacaccca tactacagca tcaagaaggt gaacaagaca 180 gtggtgccaa aggtgtctgg ctaccaatac agggtgttca aggtggtgct gcctgaccca 240 aacaagtttg ccctgcctga ctcctccctg tttgacccaa ccacccagag actggtgtgg 300 gcttgtactg gattggaggt gggcagggga caaccactgg gagtgggagt gtctggacac 360 ccactgctga acaaatatga tgatgtggag aactctggag gctatggagg caaccctgga 420 caagacaaca gggtgaatgt ggggatggac tacaagcaga cccaactttg tatggtgggc 480 tgtgcccctc cactgggaga acactggggc aagggcaccc agtgtagcaa cacctctgtc 540 cagaatggag actgtcctcc attggaactg attacctctg tgattcagga tggagatatg 600 gtggacacag gctttggagc tatgaacttt gctgacctcc aaaccaacaa gtctgatgtg 660 ccactggaca tctgtggcac agtgtgtaaa taccctgact acctccaaat ggctgctgac 720 ccatatggag acagactgtt cttctacctg aggaaggaac agatgtttgc cagacacttc 780 ttcaacaggg ctggcacagt gggagaacct gtgcctgatg acctgctggt gaagggaggc 840 aacaacaggt cctctgtggc atccagcatc tatgtgcata caccatctgg ctccctggtg 900 tcctctgagg ctcaactttt caacaagcca tactggctcc aaaaggctca aggacacaac 960 aatggcatct gttggggcaa ccacctgttt gtgacagtgg tggacaccac caggagcacc 1020 aatatgaccc tgtgtgcctc tgtgagcaag tctgccacct acaccaactc tgactacaag 1080 gaatatatga ggcatgtgga ggagtttgac ctccaattca tcttccaact ttgtagcatc 1140 accctgtctg ctgaggtgat ggcttacatc cacacaatga acccatctgt gttggaggac 1200 tggaactttg gactgagccc tcctccaaat ggcaccttgg aggacaccta cagatatgtc 1260 cagagccagg ctatcacttg tcagaagcca acacctgaga aggagaagca ggacccatac 1320 aaggatatga gtttctggga ggtgaacctg aaagagaagt tctcctctga actggaccag 1380 tttccactgg gcaggaagtt cctgctccaa tctggctaca ggggcaggac ctctgccagg 1440 acaggcatca agagacctgc tgtgagcaag ccaagcacag ccccaaagag gaagaggacc 1500 aagaccaaga agtaaactcg agctc 1525 <![CDATA[<210> 19]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1106 合成的HPV33L1基因]]> <![CDATA[<400> 19]]> ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct 120 ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat 180 gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga 240 ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag 300 agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc 360 atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct 420 ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt 480 tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc 540 aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat 600 ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag 660 tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg 720 acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg 780 agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc 840 aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc 900 agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc 1020 aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag 1080 aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt 1140 tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc 1200 ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac 1260 aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag 1320 gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac 1380 ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca 1440 aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg 1500 aagaagtaaa ctcgagctc 1519 <![CDATA[<210> 20]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1107 HPV11L1 F1]]> <![CDATA[<400> 20]]> cttggtacca tgtggagacc atctgacagc acagt 35 <![CDATA[<21]]>0> 21]]> <br/><![CDATA[<211> 36]]> <br/><![CDATA[<212> DNA]]> <br/><![CDATA[<213> 人工序列]]> <br/> <br/> <br/><![CDATA[<220> ]]> <br/><![CDATA[<223> 1108 HPV11L1 R1]]> <br/> <br/><![CDATA[<400> 21]]> <br/><![CDATA[gcttggcttt gtagccagat tggagcagga acttcc 36 <![CDATA[<210> 22]]> <![CDATA[<211> 1429]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1109 HPV11L1擴增序列1]]> <![CDATA[<400> 22]]> cttggtacca tgtggagacc atctgacagc acagtctatg tgcctcctcc aaaccctgtg 60 agcaaggtgg tggctacaga tgcctatgtg aagaggacca acatcttcta ccatgcctcc 120 tccagcagac tgctggctgt gggacaccca tactacagca tcaagaaggt gaacaagaca 180 gtggtgccaa aggtgtctgg ctaccaatac agggtgttca aggtggtgct gcctgaccca 240 aacaagtttg ccctgcctga ctcctccctg tttgacccaa ccacccagag actggtgtgg 300 gcttgtactg gattggaggt gggcagggga caaccactgg gagtgggagt gtctggacac 360 ccactgctga acaaatatga tgatgtggag aactctggag gctatggagg caaccctgga 420 caagacaaca gggtgaatgt ggggatggac tacaagcaga cccaactttg tatggtgggc 480 tgtgcccctc cactgggaga acactggggc aagggcaccc agtgtagcaa cacctctgtc 540 cagaatggag actgtcctcc attggaactg attacctctg tgattcagga tggagatatg 600 gtggacacag gctttggagc tatgaacttt gctgacctcc aaaccaacaa gtctgatgtg 660 ccactggaca tctgtggcac agtgtgtaaa taccctgact acctccaaat ggctgctgac 720 ccatatggag acagactgtt cttctacctg aggaaggaac agatgtttgc cagacacttc 780 ttcaacaggg ctggcacagt gggagaacct gtgcctgatg acctgctggt gaagggaggc 840 aacaacaggt cctctgtggc atccagcatc tatgtgcata caccatctgg ctccctggtg 900 tcctctgagg ctcaactttt caacaagcca tactggctcc aaaaggctca aggacacaac 960 aatggcatct gttggggcaa ccacctgttt gtgacagtgg tggacaccac caggagcacc 1020 aatatgaccc tgtgtgcctc tgtgagcaag tctgccacct acaccaactc tgactacaag 1080 gaatatatga ggcatgtgga ggagtttgac ctccaattca tcttccaact ttgtagcatc 1140 accctgtctg ctgaggtgat ggcttacatc cacacaatga acccatctgt gttggaggac 1200 tggaactttg gactgagccc tcctccaaat ggcaccttgg aggacaccta cagatatgtc 1260 cagagccagg ctatcacttg tcagaagcca acacctgaga aggagaagca ggacccatac 1320 aaggatatga gtttctggga ggtgaacctg aaagagaagt tctcctctga actggaccag 1380 tttccactgg gcaggaagtt cctgctccaa tctggctaca aagccaagc 1429 <![CDATA[<210> 23]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1110 HPV11L1 F2]]> <![CDATA[<400> 23]]> atctggctac aaagccaagc caaaactgaa aaggg 35 <![CDATA[<210> 24]]> <![CDATA[<211]]>> 37]]> <br/><![CDATA[<212> DNA]]> <br/><![CDATA[<213> 人工序列]]> <br/> <br/> <br/><![CDATA[<220> ]]> <br/><![CDATA[<223> 1111 HPV11L1 R2]]> <br/> <br/><![CDATA[<400> 24]]> <br/><![CDATA[ctgtctagat ttacttcttc accttcttcc tcttggc 37 <![CDATA[<210> 25]]> <![CDATA[<211> 101]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1112 HPV11L1擴增序列2]]> <![CDATA[<400> 25]]> atctggctac aaagccaagc caaaactgaa aagggctgcc ccaaccagca ccaggacctc 60 ctctgccaag aggaagaagg tgaagaagta aatctagaca g 101 <![CDATA[<210> 26]]> <![CDATA[<211> 38]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 1113 HPV 59型L1蛋白的471-508胺基酸序列]]> <![CDATA[<400> 26]]> Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 1 5 10 15 Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 20 25 30 Arg Lys Ser Ser Arg Lys 35 <![CDATA[<210> 27]]> <![CDATA[<211> 474]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 1601 HPV 16型L1蛋白的1-474胺基酸序列]]> <![CDATA[<400> 27]]> Met Ser Leu Trp Leu Pro Ser Glu Ala Thr Val Tyr Leu Pro Pro Val 1 5 10 15 Pro Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Ala Arg Thr Asn 20 25 30 Ile Tyr Tyr His Ala Gly Thr Ser Arg Leu Leu Ala Val Gly His Pro 35 40 45 Tyr Phe Pro Ile Lys Lys Pro Asn Asn Asn Lys Ile Leu Val Pro Lys 50 55 60 Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Ile His Leu Pro Asp Pro 65 70 75 80 Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asn Pro Asp Thr Gln 85 90 95 Arg Leu Val Trp Ala Cys Val Gly Val Glu Val Gly Arg Gly Gln Pro 100 105 110 Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys Leu Asp Asp 115 120 125 Thr Glu Asn Ala Ser Ala Tyr Ala Ala Asn Ala Gly Val Asp Asn Arg 130 135 140 Glu Cys Ile Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Ile Gly 145 150 155 160 Cys Lys Pro Pro Ile Gly Glu His Trp Gly Lys Gly Ser Pro Cys Thr 165 170 175 Asn Val Ala Val Asn Pro Gly Asp Cys Pro Pro Leu Glu Leu Ile Asn 180 185 190 Thr Val Ile Gln Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala Met 195 200 205 Asp Phe Thr Thr Leu Gln Ala Asn Lys Ser Glu Val Pro Leu Asp Ile 210 215 220 Cys Thr Ser Ile Cys Lys Tyr Pro Asp Tyr Ile Lys Met Val Ser Glu 225 230 235 240 Pro Tyr Gly Asp Ser Leu Phe Phe Tyr Leu Arg Arg Glu Gln Met Phe 245 250 255 Val Arg His Leu Phe Asn Arg Ala Gly Ala Val Gly Glu Asn Val Pro 260 265 270 Asp Asp Leu Tyr Ile Lys Gly Ser Gly Ser Thr Ala Asn Leu Ala Ser 275 280 285 Ser Asn Tyr Phe Pro Thr Pro Ser Gly Ser Met Val Thr Ser Asp Ala 290 295 300 Gln Ile Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn 305 310 315 320 Asn Gly Ile Cys Trp Gly Asn Gln Leu Phe Val Thr Val Val Asp Thr 325 330 335 Thr Arg Ser Thr Asn Met Ser Leu Cys Ala Ala Ile Ser Thr Ser Glu 340 345 350 Thr Thr Tyr Lys Asn Thr Asn Phe Lys Glu Tyr Leu Arg His Gly Glu 355 360 365 Glu Tyr Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu Thr 370 375 380 Ala Asp Val Met Thr Tyr Ile His Ser Met Asn Ser Thr Ile Leu Glu 385 390 395 400 Asp Trp Asn Phe Gly Leu Gln Pro Pro Pro Gly Gly Thr Leu Glu Asp 405 410 415 Thr Tyr Arg Phe Val Thr Ser Gln Ala Ile Ala Cys Gln Lys His Thr 420 425 430 Pro Pro Ala Pro Lys Glu Asp Pro Leu Lys Lys Tyr Thr Phe Trp Glu 435 440 445 Val Asn Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro Leu 450 455 460 Gly Arg Lys Phe Leu Leu Gln Ala Gly Leu 465 470 <![CDATA[<210> 28]]> <![CDATA[<211> 26]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 1602 HPV 33型L1蛋白的474-499胺基酸序列]]> <![CDATA[<400> 28]]> Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr 1 5 10 15 Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 20 25 <![CDATA[<210> 29]]> <![CDATA[<211> 500]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1603 嵌合的HPV 16型L1蛋白的胺基酸序列]]> <![CDATA[<400> 29]]> Met Ser Leu Trp Leu Pro Ser Glu Ala Thr Val Tyr Leu Pro Pro Val 1 5 10 15 Pro Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Ala Arg Thr Asn 20 25 30 Ile Tyr Tyr His Ala Gly Thr Ser Arg Leu Leu Ala Val Gly His Pro 35 40 45 Tyr Phe Pro Ile Lys Lys Pro Asn Asn Asn Lys Ile Leu Val Pro Lys 50 55 60 Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Ile His Leu Pro Asp Pro 65 70 75 80 Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asn Pro Asp Thr Gln 85 90 95 Arg Leu Val Trp Ala Cys Val Gly Val Glu Val Gly Arg Gly Gln Pro 100 105 110 Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys Leu Asp Asp 115 120 125 Thr Glu Asn Ala Ser Ala Tyr Ala Ala Asn Ala Gly Val Asp Asn Arg 130 135 140 Glu Cys Ile Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Ile Gly 145 150 155 160 Cys Lys Pro Pro Ile Gly Glu His Trp Gly Lys Gly Ser Pro Cys Thr 165 170 175 Asn Val Ala Val Asn Pro Gly Asp Cys Pro Pro Leu Glu Leu Ile Asn 180 185 190 Thr Val Ile Gln Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala Met 195 200 205 Asp Phe Thr Thr Leu Gln Ala Asn Lys Ser Glu Val Pro Leu Asp Ile 210 215 220 Cys Thr Ser Ile Cys Lys Tyr Pro Asp Tyr Ile Lys Met Val Ser Glu 225 230 235 240 Pro Tyr Gly Asp Ser Leu Phe Phe Tyr Leu Arg Arg Glu Gln Met Phe 245 250 255 Val Arg His Leu Phe Asn Arg Ala Gly Ala Val Gly Glu Asn Val Pro 260 265 270 Asp Asp Leu Tyr Ile Lys Gly Ser Gly Ser Thr Ala Asn Leu Ala Ser 275 280 285 Ser Asn Tyr Phe Pro Thr Pro Ser Gly Ser Met Val Thr Ser Asp Ala 290 295 300 Gln Ile Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn 305 310 315 320 Asn Gly Ile Cys Trp Gly Asn Gln Leu Phe Val Thr Val Val Asp Thr 325 330 335 Thr Arg Ser Thr Asn Met Ser Leu Cys Ala Ala Ile Ser Thr Ser Glu 340 345 350 Thr Thr Tyr Lys Asn Thr Asn Phe Lys Glu Tyr Leu Arg His Gly Glu 355 360 365 Glu Tyr Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu Thr 370 375 380 Ala Asp Val Met Thr Tyr Ile His Ser Met Asn Ser Thr Ile Leu Glu 385 390 395 400 Asp Trp Asn Phe Gly Leu Gln Pro Pro Pro Gly Gly Thr Leu Glu Asp 405 410 415 Thr Tyr Arg Phe Val Thr Ser Gln Ala Ile Ala Cys Gln Lys His Thr 420 425 430 Pro Pro Ala Pro Lys Glu Asp Pro Leu Lys Lys Tyr Thr Phe Trp Glu 435 440 445 Val Asn Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro Leu 450 455 460 Gly Arg Lys Phe Leu Leu Gln Ala Gly Leu Lys Ala Lys Pro Lys Leu 465 470 475 480 Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr Ser Ser Ala Lys Arg Lys 485 490 495 Lys Val Lys Lys 500 <![CDATA[<210> 30]]> <![CDATA[<211>]]> 1504 <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1604 嵌合的HPV 16型L1蛋白的核苷酸序列]]> <![CDATA[<400> 30]]> atgagtctgt ggctgccatc tgaggctaca gtctacctgc ctcctgtgcc tgtgagcaag 60 gtggtgagca cagatgaata tgtggcaagg accaacatct actaccatgc tggcaccagc 120 agactgctgg ctgtgggaca cccatacttt ccaatcaaga agccaaacaa caacaagatt 180 ctggtgccaa aggtgtctgg actccaatac agggtgttca ggattcacct gcctgaccca 240 aacaagtttg gctttcctga cacctccttc tacaaccctg acacccagag actggtgtgg 300 gcttgtgtgg gagtggaggt gggcagggga caaccactgg gagtgggcat ctctggacac 360 ccactgctga acaaactgga tgacacagag aatgcctctg cctatgctgc caatgctgga 420 gtggacaaca gggagtgtat cagtatggac tacaagcaga cccaactttg tctgattggc 480 tgtaagcctc caattggaga acactggggc aagggcagcc catgtaccaa tgtggctgtg 540 aaccctggag actgtcctcc attggaactg ataaacacag tgattcagga tggagatatg 600 gtggacacag gctttggagc tatggacttc accaccctcc aagccaacaa gtctgaggtg 660 ccactggaca tctgtaccag catctgtaaa taccctgact acatcaagat ggtgtctgaa 720 ccatatggag actccctgtt cttctacctg aggagggaac agatgtttgt gagacacctg 780 ttcaacaggg ctggagcagt gggagagaat gtgcctgatg acctctacat caagggctct 840 ggcagcacag ccaacctggc atccagcaac tactttccaa caccatctgg cagtatggtg 900 acctctgatg cccagatttt caacaagcca tactggctcc aaagggctca aggacacaac 960 aatggcatct gttggggcaa ccaacttttt gtgacagtgg tggacaccac caggagcacc 1020 aatatgagtc tgtgtgctgc catcagcacc tctgagacca cctacaagaa caccaacttc 1080 aaggaatacc tgagacatgg agaggaatat gacctccaat tcatcttcca actttgtaag 1140 attaccctga cagcagatgt gatgacctac atccacagta tgaacagcac catcttggag 1200 gactggaact ttggactcca acctcctcct ggaggcacct tggaggacac ctacaggttt 1260 gtgaccagcc aggctattgc ctgtcagaaa cacacacctc ctgccccaaa ggaggaccca 1320 ctgaaaaaat acaccttctg ggaggtgaac ctgaaagaga agttctctgc tgacctggac 1380 cagtttccac tgggcaggaa gttcctgctc caagcaggac tgaaagccaa gccaaaactg 1440 aaaagggctg ccccaaccag caccaggacc tcctctgcca agaggaagaa ggtgaagaag 1500 taaa 1504 <![CDATA[<210> 31]]> <![CDATA[<211> 1537]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1605 合成的HPV16L1基因]]> <![CDATA[<400> 31]]> ctgggtacca tgagtctgtg gctgccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtggcaagga ccaacatcta ctaccatgct 120 ggcaccagca gactgctggc tgtgggacac ccatactttc caatcaagaa gccaaacaac 180 aacaagattc tggtgccaaa ggtgtctgga ctccaataca gggtgttcag gattcacctg 240 cctgacccaa acaagtttgg ctttcctgac acctccttct acaaccctga cacccagaga 300 ctggtgtggg cttgtgtggg agtggaggtg ggcaggggac aaccactggg agtgggcatc 360 tctggacacc cactgctgaa caaactggat gacacagaga atgcctctgc ctatgctgcc 420 aatgctggag tggacaacag ggagtgtatc agtatggact acaagcagac ccaactttgt 480 ctgattggct gtaagcctcc aattggagaa cactggggca agggcagccc atgtaccaat 540 gtggctgtga accctggaga ctgtcctcca ttggaactga taaacacagt gattcaggat 600 ggagatatgg tggacacagg ctttggagct atggacttca ccaccctcca agccaacaag 660 tctgaggtgc cactggacat ctgtaccagc atctgtaaat accctgacta catcaagatg 720 gtgtctgaac catatggaga ctccctgttc ttctacctga ggagggaaca gatgtttgtg 780 agacacctgt tcaacagggc tggagcagtg ggagagaatg tgcctgatga cctctacatc 840 aagggctctg gcagcacagc caacctggca tccagcaact actttccaac accatctggc 900 agtatggtga cctctgatgc ccagattttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caactttttg tgacagtggt ggacaccacc 1020 aggagcacca atatgagtct gtgtgctgcc atcagcacct ctgagaccac ctacaagaac 1080 accaacttca aggaatacct gagacatgga gaggaatatg acctccaatt catcttccaa 1140 ctttgtaaga ttaccctgac agcagatgtg atgacctaca tccacagtat gaacagcacc 1200 atcttggagg actggaactt tggactccaa cctcctcctg gaggcacctt ggaggacacc 1260 tacaggtttg tgaccagcca ggctattgcc tgtcagaaac acacacctcc tgccccaaag 1320 gaggacccac tgaaaaaata caccttctgg gaggtgaacc tgaaagagaa gttctctgct 1380 gacctggacc agtttccact gggcaggaag ttcctgctcc aagcaggact gaaagccaag 1440 ccaaagttca ccctgggcaa gaggaaggct acaccaacca cctccagcac cagcaccaca 1500 gccaagagga agaagaggaa actgtaaact cgagctc 1537 <![CDATA[<210> 32]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1606 合成的HPV33L1基因]]> <![CDATA[<400> 32]]> ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct 120 ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat 180 gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga 240 ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag 300 agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc 360 atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct 420 ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt 480 tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc 540 aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat 600 ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag 660 tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg 720 acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg 780 agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc 840 aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc 900 agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc 1020 aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag 1080 aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt 1140 tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc 1200 ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac 1260 aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag 1320 gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac 1380 ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca 1440 aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg 1500 aagaagtaaa ctcgagctc 1519 <![CDATA[<210> 33]]> <![CDATA[<211> 34]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1607 HPV16L1 F1]]> <![CDATA[<400> 33]]> cttggtacca tgagtctgtg gctgccatct gagg 34 <![CDATA[<210> 34]]> <![CDATA[<211> 36]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1608 HPV16L1 R1]]> <![CDATA[<400> 34]]> gcttggcttt cagtcctgct tggagcagga acttcc 36 <![CDATA[<210> 35]]> <![CDATA[<211> 1441]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1609 HPV16L1擴增序列1]]> <![CDATA[<400> 35]]> cttggtacca tgagtctgtg gctgccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtggcaagga ccaacatcta ctaccatgct 120 ggcaccagca gactgctggc tgtgggacac ccatactttc caatcaagaa gccaaacaac 180 aacaagattc tggtgccaaa ggtgtctgga ctccaataca gggtgttcag gattcacctg 240 cctgacccaa acaagtttgg ctttcctgac acctccttct acaaccctga cacccagaga 300 ctggtgtggg cttgtgtggg agtggaggtg ggcaggggac aaccactggg agtgggcatc 360 tctggacacc cactgctgaa caaactggat gacacagaga atgcctctgc ctatgctgcc 420 aatgctggag tggacaacag ggagtgtatc agtatggact acaagcagac ccaactttgt 480 ctgattggct gtaagcctcc aattggagaa cactggggca agggcagccc atgtaccaat 540 gtggctgtga accctggaga ctgtcctcca ttggaactga taaacacagt gattcaggat 600 ggagatatgg tggacacagg ctttggagct atggacttca ccaccctcca agccaacaag 660 tctgaggtgc cactggacat ctgtaccagc atctgtaaat accctgacta catcaagatg 720 gtgtctgaac catatggaga ctccctgttc ttctacctga ggagggaaca gatgtttgtg 780 agacacctgt tcaacagggc tggagcagtg ggagagaatg tgcctgatga cctctacatc 840 aagggctctg gcagcacagc caacctggca tccagcaact actttccaac accatctggc 900 agtatggtga cctctgatgc ccagattttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caactttttg tgacagtggt ggacaccacc 1020 aggagcacca atatgagtct gtgtgctgcc atcagcacct ctgagaccac ctacaagaac 1080 accaacttca aggaatacct gagacatgga gaggaatatg acctccaatt catcttccaa 1140 ctttgtaaga ttaccctgac agcagatgtg atgacctaca tccacagtat gaacagcacc 1200 atcttggagg actggaactt tggactccaa cctcctcctg gaggcacctt ggaggacacc 1260 tacaggtttg tgaccagcca ggctattgcc tgtcagaaac acacacctcc tgccccaaag 1320 gaggacccac tgaaaaaata caccttctgg gaggtgaacc tgaaagagaa gttctctgct 1380 gacctggacc agtttccact gggcaggaag ttcctgctcc aagcaggact gaaagccaag 1440 c 1441 <![CDATA[<210> 36]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1610 HPV16L1 F2]]> <![CDATA[<400> 36]]> agcaggactg aaagccaagc caaaactgaa aaggg 35 <![CDATA[<210> 37]]> <![CDATA[<211> 36]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1611 HPV16L1 R2]]> <![CDATA[<400> 37]]> ctgtctagat ttacttcttc accttcttcc tcttgg 36 <![CDATA[<210> 38]]> <![CDATA[<211> 101]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1612 HPV16L1擴增序列2]]> <![CDATA[<400> 38]]> agcaggactg aaagccaagc caaaactgaa aagggctgcc ccaaccagca ccaggacctc 60 ctctgccaag aggaagaagg tgaagaagta aatctagaca g 101 <![CDATA[<210> 39]]> <![CDATA[<211> 38]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 1613 HPV 59型L1蛋白的471-508胺基酸序列]]> <![CDATA[<400> 39]]> Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 1 5 10 15 Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 20 25 30 Arg Lys Ser Ser Arg Lys 35 <![CDATA[<210> 40]]> <![CDATA[<211> 470]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 1801 HPV 18型L1蛋白的1-470胺基酸序列]]> <![CDATA[<400> 40]]> Met Ala Leu Trp Arg Pro Ser Asp Asn Thr Val Tyr Leu Pro Pro Pro 1 5 10 15 Ser Val Ala Arg Val Val Asn Thr Asp Asp Tyr Val Thr Arg Thr Ser 20 25 30 Ile Phe Tyr His Ala Gly Ser Ser Arg Leu Leu Thr Val Gly Asn Pro 35 40 45 Tyr Phe Arg Val Pro Ala Gly Gly Gly Asn Lys Gln Asp Ile Pro Lys 50 55 60 Val Ser Ala Tyr Gln Tyr Arg Val Phe Arg Val Gln Leu Pro Asp Pro 65 70 75 80 Asn Lys Phe Gly Leu Pro Asp Thr Ser Ile Tyr Asn Pro Glu Thr Gln 85 90 95 Arg Leu Val Trp Ala Cys Ala Gly Val Glu Ile Gly Arg Gly Gln Pro 100 105 110 Leu Gly Val Gly Leu Ser Gly His Pro Phe Tyr Asn Lys Leu Asp Asp 115 120 125 Thr Glu Ser Ser His Ala Ala Thr Ser Asn Val Ser Glu Asp Val Arg 130 135 140 Asp Asn Val Ser Val Asp Tyr Lys Gln Thr Gln Leu Cys Ile Leu Gly 145 150 155 160 Cys Ala Pro Ala Ile Gly Glu His Trp Ala Lys Gly Thr Ala Cys Lys 165 170 175 Ser Arg Pro Leu Ser Gln Gly Asp Cys Pro Pro Leu Glu Leu Lys Asn 180 185 190 Thr Val Leu Glu Asp Gly Asp Met Val Asp Thr Gly Tyr Gly Ala Met 195 200 205 Asp Phe Ser Thr Leu Gln Asp Thr Lys Cys Glu Val Pro Leu Asp Ile 210 215 220 Cys Gln Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Gln Met Ser Ala Asp 225 230 235 240 Pro Tyr Gly Asp Ser Met Phe Phe Cys Leu Arg Arg Glu Gln Leu Phe 245 250 255 Ala Arg His Phe Trp Asn Arg Ala Gly Thr Met Gly Asp Thr Val Pro 260 265 270 Gln Ser Leu Tyr Ile Lys Gly Thr Gly Met Arg Ala Ser Pro Gly Ser 275 280 285 Cys Val Tyr Ser Pro Ser Pro Ser Gly Ser Ile Val Thr Ser Asp Ser 290 295 300 Gln Leu Phe Asn Lys Pro Tyr Trp Leu His Lys Ala Gln Gly His Asn 305 310 315 320 Asn Gly Val Cys Trp His Asn Gln Leu Phe Val Thr Val Val Asp Thr 325 330 335 Thr Arg Ser Thr Asn Leu Thr Ile Cys Ala Ser Thr Gln Ser Pro Val 340 345 350 Pro Gly Gln Tyr Asp Ala Thr Lys Phe Lys Gln Tyr Ser Arg His Val 355 360 365 Glu Glu Tyr Asp Leu Gln Phe Ile Phe Gln Leu Cys Thr Ile Thr Leu 370 375 380 Thr Ala Asp Val Met Ser Tyr Ile His Ser Met Asn Ser Ser Ile Leu 385 390 395 400 Glu Asp Trp Asn Phe Gly Val Pro Pro Pro Pro Thr Thr Ser Leu Val 405 410 415 Asp Thr Tyr Arg Phe Val Gln Ser Val Ala Ile Thr Cys Gln Lys Asp 420 425 430 Ala Ala Pro Ala Glu Asn Lys Asp Pro Tyr Asp Lys Leu Lys Phe Trp 435 440 445 Asn Val Asp Leu Lys Glu Lys Phe Ser Leu Asp Leu Asp Gln Tyr Pro 450 455 460 Leu Gly Arg Lys Phe Leu 465 470 <![CDATA[<210> 41]]> <![CDATA[<211> 26]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 1802 HPV 33型L1蛋白的474-499胺基酸序列]]> <![CDATA[<400> 41]]> Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr 1 5 10 15 Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 20 25 <![CDATA[<210> 42]]> <![CDATA[<211> 496]]> <![CDATA[<212> PRT]]> <![CDATA[<213> ]]>人工序列 <![CDATA[<220> ]]> <![CDATA[<223> 1803 嵌合的HPV 18型L1蛋白的胺基酸序列]]> <![CDATA[<400> 42]]> Met Ala Leu Trp Arg Pro Ser Asp Asn Thr Val Tyr Leu Pro Pro Pro 1 5 10 15 Ser Val Ala Arg Val Val Asn Thr Asp Asp Tyr Val Thr Arg Thr Ser 20 25 30 Ile Phe Tyr His Ala Gly Ser Ser Arg Leu Leu Thr Val Gly Asn Pro 35 40 45 Tyr Phe Arg Val Pro Ala Gly Gly Gly Asn Lys Gln Asp Ile Pro Lys 50 55 60 Val Ser Ala Tyr Gln Tyr Arg Val Phe Arg Val Gln Leu Pro Asp Pro 65 70 75 80 Asn Lys Phe Gly Leu Pro Asp Thr Ser Ile Tyr Asn Pro Glu Thr Gln 85 90 95 Arg Leu Val Trp Ala Cys Ala Gly Val Glu Ile Gly Arg Gly Gln Pro 100 105 110 Leu Gly Val Gly Leu Ser Gly His Pro Phe Tyr Asn Lys Leu Asp Asp 115 120 125 Thr Glu Ser Ser His Ala Ala Thr Ser Asn Val Ser Glu Asp Val Arg 130 135 140 Asp Asn Val Ser Val Asp Tyr Lys Gln Thr Gln Leu Cys Ile Leu Gly 145 150 155 160 Cys Ala Pro Ala Ile Gly Glu His Trp Ala Lys Gly Thr Ala Cys Lys 165 170 175 Ser Arg Pro Leu Ser Gln Gly Asp Cys Pro Pro Leu Glu Leu Lys Asn 180 185 190 Thr Val Leu Glu Asp Gly Asp Met Val Asp Thr Gly Tyr Gly Ala Met 195 200 205 Asp Phe Ser Thr Leu Gln Asp Thr Lys Cys Glu Val Pro Leu Asp Ile 210 215 220 Cys Gln Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Gln Met Ser Ala Asp 225 230 235 240 Pro Tyr Gly Asp Ser Met Phe Phe Cys Leu Arg Arg Glu Gln Leu Phe 245 250 255 Ala Arg His Phe Trp Asn Arg Ala Gly Thr Met Gly Asp Thr Val Pro 260 265 270 Gln Ser Leu Tyr Ile Lys Gly Thr Gly Met Arg Ala Ser Pro Gly Ser 275 280 285 Cys Val Tyr Ser Pro Ser Pro Ser Gly Ser Ile Val Thr Ser Asp Ser 290 295 300 Gln Leu Phe Asn Lys Pro Tyr Trp Leu His Lys Ala Gln Gly His Asn 305 310 315 320 Asn Gly Val Cys Trp His Asn Gln Leu Phe Val Thr Val Val Asp Thr 325 330 335 Thr Arg Ser Thr Asn Leu Thr Ile Cys Ala Ser Thr Gln Ser Pro Val 340 345 350 Pro Gly Gln Tyr Asp Ala Thr Lys Phe Lys Gln Tyr Ser Arg His Val 355 360 365 Glu Glu Tyr Asp Leu Gln Phe Ile Phe Gln Leu Cys Thr Ile Thr Leu 370 375 380 Thr Ala Asp Val Met Ser Tyr Ile His Ser Met Asn Ser Ser Ile Leu 385 390 395 400 Glu Asp Trp Asn Phe Gly Val Pro Pro Pro Pro Thr Thr Ser Leu Val 405 410 415 Asp Thr Tyr Arg Phe Val Gln Ser Val Ala Ile Thr Cys Gln Lys Asp 420 425 430 Ala Ala Pro Ala Glu Asn Lys Asp Pro Tyr Asp Lys Leu Lys Phe Trp 435 440 445 Asn Val Asp Leu Lys Glu Lys Phe Ser Leu Asp Leu Asp Gln Tyr Pro 450 455 460 Leu Gly Arg Lys Phe Leu Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala 465 470 475 480 Pro Thr Ser Thr Arg Thr Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 485 490 495 <![CDATA[<210> 43]]> <![CDATA[<211> 1492]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1804 嵌合的HPV 18型L1蛋白的核苷酸序列]]> <![CDATA[<400> 43]]> atggccctct ggagaccatc cgataacaca gtgtacttgc ccccacccag cgtcgcccgg 60 gtggtgaaca cagacgacta cgtcaccaga acctcaatct tctaccacgc cgggtccagc 120 cggctgctga ccgtgggcaa cccctacttc cgcgtgcccg ccggcggcgg aaacaaacaa 180 gacatcccca aagtcagcgc ctatcagtac cgggtgttcc gcgtccaact gcccgatccc 240 aacaagttcg gcctgcccga cacctccatc tacaaccccg agacccagag gctggtctgg 300 gcttgcgccg gcgtcgagat cgggaggggc caacccctgg gcgtggggtt gtccggccac 360 cccttctaca acaagctgga cgataccgag tccagccacg cagcaaccag caacgtctcc 420 gaagatgtgc gcgataacgt cagcgtggac tacaaacaaa cccaactgtg catcctggga 480 tgcgcacccg ccatcggcga gcattgggcc aaggggaccg cctgcaagag caggcccctg 540 agccaagggg actgtccacc cctggagttg aagaataccg tgctcgagga cggcgacatg 600 gtggacaccg gctacggcgc tatggatttc tccaccctcc aggacaccaa gtgcgaagtg 660 cccctcgaca tctgccaaag catctgcaag taccccgact acctccagat gagcgccgac 720 ccctacggcg acagcatgtt cttctgtctc agaagggaac aattgttcgc ccgccacttc 780 tggaaccggg ccggcacaat gggagataca gtcccccaga gcctgtacat caaggggacc 840 ggaatgaggg ccagccccgg gtcctgcgtc tacagcccaa gcccctccgg gagcatcgtc 900 acaagcgata gccaactctt caacaagccc tactggctcc acaaagccca aggccacaat 960 aacggggtgt gttggcacaa ccagctgttc gtgaccgtcg tggacacaac caggtccaca 1020 aacctgacca tctgcgccag cacccaaagc cccgtgcccg gccagtacga cgccacaaag 1080 ttcaaacaat actctcggca cgtggaagag tacgacctcc aattcatctt ccaactctgc 1140 accatcaccc tcaccgccga cgtgatgagc tacatccact ccatgaactc ctccatcctg 1200 gaagactgga atttcggcgt gccaccaccc cctaccacct ccctcgtcga cacctacaga 1260 ttcgtgcaga gcgtggccat cacatgccag aaagacgccg cccccgccga gaacaaagac 1320 ccatacgaca aactgaaatt ctggaacgtc gacctgaaag agaaattcag cctggatctg 1380 gaccagtacc cattgggcag gaagttcctc aaagccaagc caaaactgaa aagggctgcc 1440 ccaaccagca ccaggacctc ctctgccaag aggaagaagg tgaagaagta aa 1492 <![CDATA[<210> 44]]> <![CDATA[<211> 1543]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1805 合成的HPV18L1基因]]> <![CDATA[<400> 44]]> ctgggtacca tggccctctg gagaccatcc gataacacag tgtacttgcc cccacccagc 60 gtcgcccggg tggtgaacac agacgactac gtcaccagaa cctcaatctt ctaccacgcc 120 gggtccagcc ggctgctgac cgtgggcaac ccctacttcc gcgtgcccgc cggcggcgga 180 aacaaacaag acatccccaa agtcagcgcc tatcagtacc gggtgttccg cgtccaactg 240 cccgatccca acaagttcgg cctgcccgac acctccatct acaaccccga gacccagagg 300 ctggtctggg cttgcgccgg cgtcgagatc gggaggggcc aacccctggg cgtggggttg 360 tccggccacc ccttctacaa caagctggac gataccgagt ccagccacgc agcaaccagc 420 aacgtctccg aagatgtgcg cgataacgtc agcgtggact acaaacaaac ccaactgtgc 480 atcctgggat gcgcacccgc catcggcgag cattgggcca aggggaccgc ctgcaagagc 540 aggcccctga gccaagggga ctgtccaccc ctggagttga agaataccgt gctcgaggac 600 ggcgacatgg tggacaccgg ctacggcgct atggatttct ccaccctcca ggacaccaag 660 tgcgaagtgc ccctcgacat ctgccaaagc atctgcaagt accccgacta cctccagatg 720 agcgccgacc cctacggcga cagcatgttc ttctgtctca gaagggaaca attgttcgcc 780 cgccacttct ggaaccgggc cggcacaatg ggagatacag tcccccagag cctgtacatc 840 aaggggaccg gaatgagggc cagccccggg tcctgcgtct acagcccaag cccctccggg 900 agcatcgtca caagcgatag ccaactcttc aacaagccct actggctcca caaagcccaa 960 ggccacaata acggggtgtg ttggcacaac cagctgttcg tgaccgtcgt ggacacaacc 1020 aggtccacaa acctgaccat ctgcgccagc acccaaagcc ccgtgcccgg ccagtacgac 1080 gccacaaagt tcaaacaata ctctcggcac gtggaagagt acgacctcca attcatcttc 1140 caactctgca ccatcaccct caccgccgac gtgatgagct acatccactc catgaactcc 1200 tccatcctgg aagactggaa tttcggcgtg ccaccacccc ctaccacctc cctcgtcgac 1260 acctacagat tcgtgcagag cgtggccatc acatgccaga aagacgccgc ccccgccgag 1320 aacaaagacc catacgacaa actgaaattc tggaacgtcg acctgaaaga gaaattcagc 1380 ctggatctgg accagtaccc attgggcagg aagttcctcg tgcaagccgg cctcaggaga 1440 aaaccaacaa tcgggcccag gaagaggagc gcccccagcg caaccaccag cagcaagccc 1500 gcaaaaaggg tcagagtgag ggcacgcaaa taaactcgag ctc 1543 <![CDATA[<210> 45]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1806 合成的HPV33L1基因]]> <![CDATA[<400> 45]]> ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct 120 ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat 180 gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga 240 ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag 300 agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc 360 atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct 420 ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt 480 tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc 540 aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat 600 ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag 660 tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg 720 acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg 780 agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc 840 aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc 900 agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc 1020 aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag 1080 aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt 1140 tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc 1200 ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac 1260 aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag 1320 gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac 1380 ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca 1440 aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg 1500 aagaagtaaa ctcgagctc 1519 <![CDATA[<210> 46]]> <![CDATA[<211> 34]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1807 HPV18L1 F1]]> <![CDATA[<400> 46]]> cttggtacca tggccctctg gagaccatcc gata 34 <![CDATA[<210> 47]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1808 HPV18L1 R1]]> <![CDATA[<400> 47]]> gcttggcttt gaggaacttc ctgcccaatg ggtac 35 <![CDATA[<210> 48]]> <![CDATA[<211> 1429]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1809 HPV18L1擴增序列1]]> <![CDATA[<400> 48]]> cttggtacca tggccctctg gagaccatcc gataacacag tgtacttgcc cccacccagc 60 gtcgcccggg tggtgaacac agacgactac gtcaccagaa cctcaatctt ctaccacgcc 120 gggtccagcc ggctgctgac cgtgggcaac ccctacttcc gcgtgcccgc cggcggcgga 180 aacaaacaag acatccccaa agtcagcgcc tatcagtacc gggtgttccg cgtccaactg 240 cccgatccca acaagttcgg cctgcccgac acctccatct acaaccccga gacccagagg 300 ctggtctggg cttgcgccgg cgtcgagatc gggaggggcc aacccctggg cgtggggttg 360 tccggccacc ccttctacaa caagctggac gataccgagt ccagccacgc agcaaccagc 420 aacgtctccg aagatgtgcg cgataacgtc agcgtggact acaaacaaac ccaactgtgc 480 atcctgggat gcgcacccgc catcggcgag cattgggcca aggggaccgc ctgcaagagc 540 aggcccctga gccaagggga ctgtccaccc ctggagttga agaataccgt gctcgaggac 600 ggcgacatgg tggacaccgg ctacggcgct atggatttct ccaccctcca ggacaccaag 660 tgcgaagtgc ccctcgacat ctgccaaagc atctgcaagt accccgacta cctccagatg 720 agcgccgacc cctacggcga cagcatgttc ttctgtctca gaagggaaca attgttcgcc 780 cgccacttct ggaaccgggc cggcacaatg ggagatacag tcccccagag cctgtacatc 840 aaggggaccg gaatgagggc cagccccggg tcctgcgtct acagcccaag cccctccggg 900 agcatcgtca caagcgatag ccaactcttc aacaagccct actggctcca caaagcccaa 960 ggccacaata acggggtgtg ttggcacaac cagctgttcg tgaccgtcgt ggacacaacc 1020 aggtccacaa acctgaccat ctgcgccagc acccaaagcc ccgtgcccgg ccagtacgac 1080 gccacaaagt tcaaacaata ctctcggcac gtggaagagt acgacctcca attcatcttc 1140 caactctgca ccatcaccct caccgccgac gtgatgagct acatccactc catgaactcc 1200 tccatcctgg aagactggaa tttcggcgtg ccaccacccc ctaccacctc cctcgtcgac 1260 acctacagat tcgtgcagag cgtggccatc acatgccaga aagacgccgc ccccgccgag 1320 aacaaagacc catacgacaa actgaaattc tggaacgtcg acctgaaaga gaaattcagc 1380 ctggatctgg accagtaccc attgggcagg aagttcctca aagccaagc 1429 <![CDATA[<210> 49]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1810 HPV18L1 F2]]> <![CDATA[<400> 49]]> gaagttcctc aaagccaagc caaaactgaa aaggg 35 <![CDATA[<210> 50]]> <![CDATA[<211> 36]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1811 HPV18L1 R2]]> <![CDATA[<400> 50]]> ctgtctagat ttacttcttc accttcttcc tcttgg 36 <![CDATA[<210> 51]]> <![CDATA[<211> 101]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 1812 HPV18L1擴增序列2]]> <![CDATA[<400> 51]]> gaagttcctc aaagccaagc caaaactgaa aagggctgcc ccaaccagca ccaggacctc 60 ctctgccaag aggaagaagg tgaagaagta aatctagaca g 101 <![CDATA[<210> 52]]> <![CDATA[<211> 38]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 1813 HPV 59型L1蛋白的471-508胺基酸序列]]> <![CDATA[<400> 52]]> Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 1 5 10 15 Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 20 25 30 Arg Lys Ser Ser Arg Lys 35 <![CDATA[<210> 53]]> <![CDATA[<211> 475]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 3101 HPV 31型L1蛋白的1-475胺基酸序列]]> <![CDATA[<400> 53]]> Met Ser Leu Trp Arg Pro Ser Glu Ala Thr Val Tyr Leu Pro Pro Val 1 5 10 15 Pro Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Thr Arg Thr Asn 20 25 30 Ile Tyr Tyr His Ala Gly Ser Ala Arg Leu Leu Thr Val Gly His Pro 35 40 45 Tyr Tyr Ser Ile Pro Lys Ser Asp Asn Pro Lys Lys Ile Val Val Pro 50 55 60 Lys Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Val Arg Leu Pro Asp 65 70 75 80 Pro Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asn Pro Glu Thr 85 90 95 Gln Arg Leu Val Trp Ala Cys Val Gly Leu Glu Val Gly Arg Gly Gln 100 105 110 Pro Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys Phe Asp 115 120 125 Asp Thr Glu Asn Ser Asn Arg Tyr Ala Gly Gly Pro Gly Thr Asp Asn 130 135 140 Arg Glu Cys Ile Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Leu 145 150 155 160 Gly Cys Lys Pro Pro Ile Gly Glu His Trp Gly Lys Gly Ser Pro Cys 165 170 175 Ser Asn Asn Ala Ile Thr Pro Gly Asp Cys Pro Pro Leu Glu Leu Lys 180 185 190 Asn Ser Val Ile Gln Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala 195 200 205 Met Asp Phe Thr Ala Leu Gln Asp Thr Lys Ser Asn Val Pro Leu Asp 210 215 220 Ile Cys Asn Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Lys Met Val Ala 225 230 235 240 Glu Pro Tyr Gly Asp Thr Leu Phe Phe Tyr Leu Arg Arg Glu Gln Met 245 250 255 Phe Val Arg His Phe Phe Asn Arg Ser Gly Thr Val Gly Glu Ser Val 260 265 270 Pro Thr Asp Leu Tyr Ile Lys Gly Ser Gly Ser Thr Ala Thr Leu Ala 275 280 285 Asn Ser Thr Tyr Phe Pro Thr Pro Ser Gly Ser Met Val Thr Ser Asp 290 295 300 Ala Gln Ile Phe Asn Lys Pro Tyr Trp Met Gln Arg Ala Gln Gly His 305 310 315 320 Asn Asn Gly Ile Cys Trp Gly Asn Gln Leu Phe Val Thr Val Val Asp 325 330 335 Thr Thr Arg Ser Thr Asn Met Ser Val Cys Ala Ala Ile Ala Asn Ser 340 345 350 Asp Thr Thr Phe Lys Ser Ser Asn Phe Lys Glu Tyr Leu Arg His Gly 355 360 365 Glu Glu Phe Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu 370 375 380 Ser Ala Asp Ile Met Thr Tyr Ile His Ser Met Asn Pro Ala Ile Leu 385 390 395 400 Glu Asp Trp Asn Phe Gly Leu Thr Thr Pro Pro Ser Gly Ser Leu Glu 405 410 415 Asp Thr Tyr Arg Phe Val Thr Ser Gln Ala Ile Thr Cys Gln Lys Ser 420 425 430 Ala Pro Gln Lys Pro Lys Glu Asp Pro Phe Lys Asp Tyr Val Phe Trp 435 440 445 Glu Val Asn Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro 450 455 460 Leu Gly Arg Lys Phe Leu Leu Gln Ala Gly Tyr 465 470 475 <![CDATA[<210> 54]]> <![CDATA[<211> 26]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 3102 HPV 33型L1蛋白的474-499胺基酸序列]]> <![CDATA[<400> 54]]> Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr 1 5 10 15 Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 20 25 <![CDATA[<210> 55]]> <![CDATA[<211> 501]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3103 嵌合的HPV 31型L1蛋白的胺基酸序列]]> <![CDATA[<400> 55]]> Met Ser Leu Trp Arg Pro Ser Glu Ala Thr Val Tyr Leu Pro Pro Val 1 5 10 15 Pro Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Thr Arg Thr Asn 20 25 30 Ile Tyr Tyr His Ala Gly Ser Ala Arg Leu Leu Thr Val Gly His Pro 35 40 45 Tyr Tyr Ser Ile Pro Lys Ser Asp Asn Pro Lys Lys Ile Val Val Pro 50 55 60 Lys Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Val Arg Leu Pro Asp 65 70 75 80 Pro Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asn Pro Glu Thr 85 90 95 Gln Arg Leu Val Trp Ala Cys Val Gly Leu Glu Val Gly Arg Gly Gln 100 105 110 Pro Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys Phe Asp 115 120 125 Asp Thr Glu Asn Ser Asn Arg Tyr Ala Gly Gly Pro Gly Thr Asp Asn 130 135 140 Arg Glu Cys Ile Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Leu 145 150 155 160 Gly Cys Lys Pro Pro Ile Gly Glu His Trp Gly Lys Gly Ser Pro Cys 165 170 175 Ser Asn Asn Ala Ile Thr Pro Gly Asp Cys Pro Pro Leu Glu Leu Lys 180 185 190 Asn Ser Val Ile Gln Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala 195 200 205 Met Asp Phe Thr Ala Leu Gln Asp Thr Lys Ser Asn Val Pro Leu Asp 210 215 220 Ile Cys Asn Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Lys Met Val Ala 225 230 235 240 Glu Pro Tyr Gly Asp Thr Leu Phe Phe Tyr Leu Arg Arg Glu Gln Met 245 250 255 Phe Val Arg His Phe Phe Asn Arg Ser Gly Thr Val Gly Glu Ser Val 260 265 270 Pro Thr Asp Leu Tyr Ile Lys Gly Ser Gly Ser Thr Ala Thr Leu Ala 275 280 285 Asn Ser Thr Tyr Phe Pro Thr Pro Ser Gly Ser Met Val Thr Ser Asp 290 295 300 Ala Gln Ile Phe Asn Lys Pro Tyr Trp Met Gln Arg Ala Gln Gly His 305 310 315 320 Asn Asn Gly Ile Cys Trp Gly Asn Gln Leu Phe Val Thr Val Val Asp 325 330 335 Thr Thr Arg Ser Thr Asn Met Ser Val Cys Ala Ala Ile Ala Asn Ser 340 345 350 Asp Thr Thr Phe Lys Ser Ser Asn Phe Lys Glu Tyr Leu Arg His Gly 355 360 365 Glu Glu Phe Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu 370 375 380 Ser Ala Asp Ile Met Thr Tyr Ile His Ser Met Asn Pro Ala Ile Leu 385 390 395 400 Glu Asp Trp Asn Phe Gly Leu Thr Thr Pro Pro Ser Gly Ser Leu Glu 405 410 415 Asp Thr Tyr Arg Phe Val Thr Ser Gln Ala Ile Thr Cys Gln Lys Ser 420 425 430 Ala Pro Gln Lys Pro Lys Glu Asp Pro Phe Lys Asp Tyr Val Phe Trp 435 440 445 Glu Val Asn Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro 450 455 460 Leu Gly Arg Lys Phe Leu Leu Gln Ala Gly Tyr Lys Ala Lys Pro Lys 465 470 475 480 Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr Ser Ser Ala Lys Arg 485 490 495 Lys Lys Val Lys Lys 500 <![CDATA[<210> 56]]> <![CDATA[<211> 1507]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3104 嵌合的HPV 31型L1蛋白的核苷酸序列]]> <![CDATA[<400> 56]]> atgagcctgt ggaggcccag cgaggccacc gtgtacctgc cccccgtgcc cgtgagcaag 60 gtggtgagca ccgacgagta cgtgaccagg accaacatct actaccacgc cggcagcgcc 120 aggctgctga ccgtgggcca cccctactac agcatcccca agagcgacaa ccccaagaag 180 atcgtggtgc ccaaggtgag cggcctgcag tacagggtgt tcagggtgag gctgcccgac 240 cccaacaagt tcggcttccc cgacaccagc ttctacaacc ccgagaccca gaggctggtg 300 tgggcctgcg tgggcctgga ggtgggcagg ggccagcccc tgggcgtggg catcagcggc 360 caccccctgc tgaacaagtt cgacgacacc gagaacagca acaggtacgc cggcggcccc 420 ggcaccgaca acagggagtg catcagcatg gactacaagc agacccagct gtgcctgctg 480 ggctgcaagc cccccatcgg cgagcactgg ggcaagggca gcccctgcag caacaacgcc 540 atcacccccg gcgactgccc ccccctggag ctgaagaaca gcgtgatcca ggacggcgac 600 atggtggaca ccggcttcgg cgccatggac ttcaccgccc tgcaggacac caagagcaac 660 gtgcccctgg acatctgcaa cagcatctgc aagtaccccg actacctgaa gatggtggcc 720 gagccctacg gcgacaccct gttcttctac ctgaggaggg agcagatgtt cgtgaggcac 780 ttcttcaaca ggagcggcac cgtgggcgag agcgtgccca ccgacctgta catcaagggc 840 agcggcagca ccgccaccct ggccaacagc acctacttcc ccacccccag cggcagcatg 900 gtgaccagcg acgcccagat cttcaacaag ccctactgga tgcagagggc ccagggccac 960 aacaacggca tctgctgggg caaccagctg ttcgtgaccg tggtggacac caccaggagc 1020 accaacatga gcgtgtgcgc cgccatcgcc aacagcgaca ccaccttcaa gagcagcaac 1080 ttcaaggagt acctgaggca cggcgaggag ttcgacctgc agttcatctt ccagctgtgc 1140 aagatcaccc tgagcgccga catcatgacc tacatccaca gcatgaaccc cgccatcctg 1200 gaggactgga acttcggcct gaccaccccc cccagcggca gcctggagga cacctacagg 1260 ttcgtgacca gccaggccat cacctgccag aagtccgccc cccagaagcc caaggaggac 1320 cccttcaagg actacgtgtt ctgggaggtg aacctgaagg agaagttcag cgccgacctg 1380 gaccagttcc ccctgggcag gaagttcctg ctgcaggccg gctacaaagc caagccaaaa 1440 ctgaaaaggg ctgccccaac cagcaccagg acctcctctg ccaagaggaa gaaggtgaag 1500 aagtaaa 1507 <![CDATA[<210> 57]]> <![CDATA[<211> 1534]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3105 合成的HPV31L1基因]]> <![CDATA[<400> 57]]> ctgggtacca tgagcctgtg gaggcccagc gaggccaccg tgtacctgcc ccccgtgccc 60 gtgagcaagg tggtgagcac cgacgagtac gtgaccagga ccaacatcta ctaccacgcc 120 ggcagcgcca ggctgctgac cgtgggccac ccctactaca gcatccccaa gagcgacaac 180 cccaagaaga tcgtggtgcc caaggtgagc ggcctgcagt acagggtgtt cagggtgagg 240 ctgcccgacc ccaacaagtt cggcttcccc gacaccagct tctacaaccc cgagacccag 300 aggctggtgt gggcctgcgt gggcctggag gtgggcaggg gccagcccct gggcgtgggc 360 atcagcggcc accccctgct gaacaagttc gacgacaccg agaacagcaa caggtacgcc 420 ggcggccccg gcaccgacaa cagggagtgc atcagcatgg actacaagca gacccagctg 480 tgcctgctgg gctgcaagcc ccccatcggc gagcactggg gcaagggcag cccctgcagc 540 aacaacgcca tcacccccgg cgactgcccc cccctggagc tgaagaacag cgtgatccag 600 gacggcgaca tggtggacac cggcttcggc gccatggact tcaccgccct gcaggacacc 660 aagagcaacg tgcccctgga catctgcaac agcatctgca agtaccccga ctacctgaag 720 atggtggccg agccctacgg cgacaccctg ttcttctacc tgaggaggga gcagatgttc 780 gtgaggcact tcttcaacag gagcggcacc gtgggcgaga gcgtgcccac cgacctgtac 840 atcaagggca gcggcagcac cgccaccctg gccaacagca cctacttccc cacccccagc 900 ggcagcatgg tgaccagcga cgcccagatc ttcaacaagc cctactggat gcagagggcc 960 cagggccaca acaacggcat ctgctggggc aaccagctgt tcgtgaccgt ggtggacacc 1020 accaggagca ccaacatgag cgtgtgcgcc gccatcgcca acagcgacac caccttcaag 1080 agcagcaact tcaaggagta cctgaggcac ggcgaggagt tcgacctgca gttcatcttc 1140 cagctgtgca agatcaccct gagcgccgac atcatgacct acatccacag catgaacccc 1200 gccatcctgg aggactggaa cttcggcctg accacccccc ccagcggcag cctggaggac 1260 acctacaggt tcgtgaccag ccaggccatc acctgccaga agtccgcccc ccagaagccc 1320 aaggaggacc ccttcaagga ctacgtgttc tgggaggtga acctgaagga gaagttcagc 1380 gccgacctgg accagttccc cctgggcagg aagttcctgc tgcaggccgg ctacagggcc 1440 aggcccaagt tcaaggccgg caagaggagc gcccccagcg ccagcaccac cacccccgcc 1500 aagaggaaga agaccaagaa gtaaactcga gctc 1534 <![CDATA[<210> 58]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3106 合成的HPV33L1基因]]> <![CDATA[<400> 58]]> ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct 120 ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat 180 gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga 240 ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag 300 agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc 360 atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct 420 ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt 480 tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc 540 aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat 600 ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag 660 tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg 720 acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg 780 agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc 840 aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc 900 agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc 1020 aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag 1080 aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt 1140 tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc 1200 ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac 1260 aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag 1320 gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac 1380 ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca 1440 aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg 1500 aagaagtaaa ctcgagctc 1519 <![CDATA[<210> 59]]> <![CDATA[<211> 33]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3107 HPV31L1 F1]]> <![CDATA[<400> 59]]> cttggtacca tgagcctgtg gaggcccagc gag 33 <![CDATA[<210> 60]]> <![CDATA[<211> 38]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3108 HPV31L1 R1]]> <![CDATA[<400> 60]]> gcttggcttt gtagccggcc tgcagcagga acttcctg 38 <![CDATA[<210> 61]]> <![CDATA[<211> 1444]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3109 HPV31L1擴增序列1]]> <![CDATA[<400> 61]]> cttggtacca tgagcctgtg gaggcccagc gaggccaccg tgtacctgcc ccccgtgccc 60 gtgagcaagg tggtgagcac cgacgagtac gtgaccagga ccaacatcta ctaccacgcc 120 ggcagcgcca ggctgctgac cgtgggccac ccctactaca gcatccccaa gagcgacaac 180 cccaagaaga tcgtggtgcc caaggtgagc ggcctgcagt acagggtgtt cagggtgagg 240 ctgcccgacc ccaacaagtt cggcttcccc gacaccagct tctacaaccc cgagacccag 300 aggctggtgt gggcctgcgt gggcctggag gtgggcaggg gccagcccct gggcgtgggc 360 atcagcggcc accccctgct gaacaagttc gacgacaccg agaacagcaa caggtacgcc 420 ggcggccccg gcaccgacaa cagggagtgc atcagcatgg actacaagca gacccagctg 480 tgcctgctgg gctgcaagcc ccccatcggc gagcactggg gcaagggcag cccctgcagc 540 aacaacgcca tcacccccgg cgactgcccc cccctggagc tgaagaacag cgtgatccag 600 gacggcgaca tggtggacac cggcttcggc gccatggact tcaccgccct gcaggacacc 660 aagagcaacg tgcccctgga catctgcaac agcatctgca agtaccccga ctacctgaag 720 atggtggccg agccctacgg cgacaccctg ttcttctacc tgaggaggga gcagatgttc 780 gtgaggcact tcttcaacag gagcggcacc gtgggcgaga gcgtgcccac cgacctgtac 840 atcaagggca gcggcagcac cgccaccctg gccaacagca cctacttccc cacccccagc 900 ggcagcatgg tgaccagcga cgcccagatc ttcaacaagc cctactggat gcagagggcc 960 cagggccaca acaacggcat ctgctggggc aaccagctgt tcgtgaccgt ggtggacacc 1020 accaggagca ccaacatgag cgtgtgcgcc gccatcgcca acagcgacac caccttcaag 1080 agcagcaact tcaaggagta cctgaggcac ggcgaggagt tcgacctgca gttcatcttc 1140 cagctgtgca agatcaccct gagcgccgac atcatgacct acatccacag catgaacccc 1200 gccatcctgg aggactggaa cttcggcctg accacccccc ccagcggcag cctggaggac 1260 acctacaggt tcgtgaccag ccaggccatc acctgccaga agtccgcccc ccagaagccc 1320 aaggaggacc ccttcaagga ctacgtgttc tgggaggtga acctgaagga gaagttcagc 1380 gccgacctgg accagttccc cctgggcagg aagttcctgc tgcaggccgg ctacaaagcc 1440 aagc 1444 <![CDATA[<210> 62]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3110 HPV31L1 F2]]> <![CDATA[<400> 62]]> ggccggctac aaagccaagc caaaactgaa aaggg 35 <![CDATA[<210> 63]]> <![CDATA[<211> 41]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3111 HPV31L1 R2]]> <![CDATA[<400> 63]]> ctgtctagat ttacttcttc accttcttcc tcttggcaga g 41 <![CDATA[<210> 64]]> <![CDATA[<211> 101]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3112 HPV31L1擴增序列2]]> <![CDATA[<400> 64]]> ggccggctac aaagccaagc caaaactgaa aagggctgcc ccaaccagca ccaggacctc 60 ctctgccaag aggaagaagg tgaagaagta aatctagaca g 101 <![CDATA[<210> 65]]> <![CDATA[<211> 38]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 3113 HPV 59型L1蛋白的471-508胺基酸序列]]> <![CDATA[<400> 65]]> Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 1 5 10 15 Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 20 25 30 Arg Lys Ser Ser Arg Lys 35 <![CDATA[<210> 66]]> <![CDATA[<211> 499]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 3301 HPV 33型L1蛋白的胺基酸序列]]> <![CDATA[<400> 66]]> Met Ser Val Trp Arg Pro Ser Glu Ala Thr Val Tyr Leu Pro Pro Val 1 5 10 15 Pro Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Ser Arg Thr Ser 20 25 30 Ile Tyr Tyr Tyr Ala Gly Ser Ser Arg Leu Leu Ala Val Gly His Pro 35 40 45 Tyr Phe Ser Ile Lys Asn Pro Thr Asn Ala Lys Lys Leu Leu Val Pro 50 55 60 Lys Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Val Arg Leu Pro Asp 65 70 75 80 Pro Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asn Pro Asp Thr 85 90 95 Gln Arg Leu Val Trp Ala Cys Val Gly Leu Glu Ile Gly Arg Gly Gln 100 105 110 Pro Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys Phe Asp 115 120 125 Asp Thr Glu Thr Gly Asn Lys Tyr Pro Gly Gln Pro Gly Ala Asp Asn 130 135 140 Arg Glu Cys Leu Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Leu 145 150 155 160 Gly Cys Lys Pro Pro Thr Gly Glu His Trp Gly Lys Gly Val Ala Cys 165 170 175 Thr Asn Ala Ala Pro Ala Asn Asp Cys Pro Pro Leu Glu Leu Ile Asn 180 185 190 Thr Ile Ile Glu Asp Gly Asp Met Val Asp Thr Gly Phe Gly Cys Met 195 200 205 Asp Phe Lys Thr Leu Gln Ala Asn Lys Ser Asp Val Pro Ile Asp Ile 210 215 220 Cys Gly Ser Thr Cys Lys Tyr Pro Asp Tyr Leu Lys Met Thr Ser Glu 225 230 235 240 Pro Tyr Gly Asp Ser Leu Phe Phe Phe Leu Arg Arg Glu Gln Met Phe 245 250 255 Val Arg His Phe Phe Asn Arg Ala Gly Thr Leu Gly Glu Ala Val Pro 260 265 270 Asp Asp Leu Tyr Ile Lys Gly Ser Gly Thr Thr Ala Ser Ile Gln Ser 275 280 285 Ser Ala Phe Phe Pro Thr Pro Ser Gly Ser Met Val Thr Ser Glu Ser 290 295 300 Gln Leu Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn 305 310 315 320 Asn Gly Ile Cys Trp Gly Asn Gln Val Phe Val Thr Val Val Asp Thr 325 330 335 Thr Arg Ser Thr Asn Met Thr Leu Cys Thr Gln Val Thr Ser Asp Ser 340 345 350 Thr Tyr Lys Asn Glu Asn Phe Lys Glu Tyr Ile Arg His Val Glu Glu 355 360 365 Tyr Asp Leu Gln Phe Val Phe Gln Leu Cys Lys Val Thr Leu Thr Ala 370 375 380 Glu Val Met Thr Tyr Ile His Ala Met Asn Pro Asp Ile Leu Glu Asp 385 390 395 400 Trp Gln Phe Gly Leu Thr Pro Pro Pro Ser Ala Ser Leu Gln Asp Thr 405 410 415 Tyr Arg Phe Val Thr Ser Gln Ala Ile Thr Cys Gln Lys Thr Val Pro 420 425 430 Pro Lys Glu Lys Glu Asp Pro Leu Gly Lys Tyr Thr Phe Trp Glu Val 435 440 445 Asp Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro Leu Gly 450 455 460 Arg Lys Phe Leu Leu Gln Ala Gly Leu Lys Ala Lys Pro Lys Leu Lys 465 470 475 480 Arg Ala Ala Pro Thr Ser Thr Arg Thr Ser Ser Ala Lys Arg Lys Lys 485 490 495 Val Lys Lys <![CDATA[<210> 67]]> <![CDATA[<211> 1501]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 3302 HPV 33型L1蛋白的核苷酸序列]]> <![CDATA[<400> 67]]> atgagtgtgt ggagaccatc tgaggctaca gtctacctgc ctcctgtgcc tgtgagcaag 60 gtggtgagca cagatgaata tgtgagcagg accagcatct actactatgc tggctccagc 120 agactgctgg ctgtgggaca cccatacttc agcatcaaga acccaaccaa tgccaagaaa 180 ctgctggtgc caaaggtgtc tggactccaa tacagggtgt tcagggtgag actgcctgac 240 ccaaacaagt ttggctttcc tgacacctcc ttctacaacc ctgacaccca gagactggtg 300 tgggcttgtg tgggattgga gattggcagg ggacaaccac tgggagtggg catctctgga 360 cacccactgc tgaacaagtt tgatgacaca gagacaggca acaaataccc tggacaacct 420 ggagcagaca acagggagtg tctgagtatg gactacaagc agacccaact ttgtctgctg 480 ggctgtaagc ctccaacagg agaacactgg ggcaagggag tggcttgtac caatgctgcc 540 cctgccaatg actgtcctcc attggaactg ataaacacca tcattgagga tggagatatg 600 gtggacacag gctttggctg tatggacttc aagaccctcc aagccaacaa gtctgatgtg 660 ccaattgaca tctgtggcag cacttgtaaa taccctgact acctgaaaat gacctctgaa 720 ccatatggag actccctgtt cttcttcctg aggagggaac agatgtttgt gagacacttc 780 ttcaacaggg ctggcaccct gggagaggct gtgcctgatg acctctacat caagggctct 840 ggcaccacag ccagcatcca gtcctctgcc ttctttccaa caccatctgg cagtatggtg 900 acctctgaga gccaactttt caacaagcca tactggctcc aaagggctca aggacacaac 960 aatggcatct gttggggcaa ccaggtgttt gtgacagtgg tggacaccac caggagcacc 1020 aatatgaccc tgtgtaccca ggtgacctct gacagcacct acaagaatga gaacttcaag 1080 gaatacatca ggcatgtgga ggaatatgac ctccaatttg tgttccaact ttgtaaggtg 1140 accctgacag cagaggtgat gacctacatc catgctatga accctgacat cttggaggac 1200 tggcagtttg gactgacacc tcctccatct gcctccctcc aagacaccta caggtttgtg 1260 accagccagg ctatcacttg tcagaagaca gtgcctccaa aggagaagga ggacccactg 1320 ggcaaataca ccttctggga ggtggacctg aaagagaagt tctctgctga cctggaccag 1380 tttccactgg gcaggaagtt cctgctccaa gcaggactga aagccaagcc aaaactgaaa 1440 agggctgccc caaccagcac caggacctcc tctgccaaga ggaagaaggt gaagaagtaa 1500 a 1501 <![CDATA[<210> 68]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3303 合成的HPV33L1基因]]> <![CDATA[<400> 68]]> ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct 120 ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat 180 gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga 240 ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag 300 agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc 360 atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct 420 ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt 480 tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc 540 aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat 600 ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag 660 tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg 720 acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg 780 agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc 840 aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc 900 agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc 1020 aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag 1080 aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt 1140 tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc 1200 ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac 1260 aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag 1320 gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac 1380 ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca 1440 aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg 1500 aagaagtaaa ctcgagctc 1519 <![CDATA[<210> 69]]> <![CDATA[<211> 472]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 3501 HPV 35型L1蛋白的1-472胺基酸序列]]> <![CDATA[<400> 69]]> Met Ser Leu Trp Arg Ser Asn Glu Ala Thr Val Tyr Leu Pro Pro Val 1 5 10 15 Ser Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Thr Arg Thr Asn 20 25 30 Ile Tyr Tyr His Ala Gly Ser Ser Arg Leu Leu Ala Val Gly His Pro 35 40 45 Tyr Tyr Ala Ile Lys Lys Gln Asp Ser Asn Lys Ile Ala Val Pro Lys 50 55 60 Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Val Lys Leu Pro Asp Pro 65 70 75 80 Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asp Pro Ala Ser Gln 85 90 95 Arg Leu Val Trp Ala Cys Thr Gly Val Glu Val Gly Arg Gly Gln Pro 100 105 110 Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys Leu Asp Asp 115 120 125 Thr Glu Asn Ser Asn Lys Tyr Val Gly Asn Ser Gly Thr Asp Asn Arg 130 135 140 Glu Cys Ile Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Ile Gly 145 150 155 160 Cys Arg Pro Pro Ile Gly Glu His Trp Gly Lys Gly Thr Pro Cys Asn 165 170 175 Ala Asn Gln Val Lys Ala Gly Glu Cys Pro Pro Leu Glu Leu Leu Asn 180 185 190 Thr Val Leu Gln Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala Met 195 200 205 Asp Phe Thr Thr Leu Gln Ala Asn Lys Ser Asp Val Pro Leu Asp Ile 210 215 220 Cys Ser Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Lys Met Val Ser Glu 225 230 235 240 Pro Tyr Gly Asp Met Leu Phe Phe Tyr Leu Arg Arg Glu Gln Met Phe 245 250 255 Val Arg His Leu Phe Asn Arg Ala Gly Thr Val Gly Glu Thr Val Pro 260 265 270 Ala Asp Leu Tyr Ile Lys Gly Thr Thr Gly Thr Leu Pro Ser Thr Ser 275 280 285 Tyr Phe Pro Thr Pro Ser Gly Ser Met Val Thr Ser Asp Ala Gln Ile 290 295 300 Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn Asn Gly 305 310 315 320 Ile Cys Trp Ser Asn Gln Leu Phe Val Thr Val Val Asp Thr Thr Arg 325 330 335 Ser Thr Asn Met Ser Val Cys Ser Ala Val Ser Ser Ser Asp Ser Thr 340 345 350 Tyr Lys Asn Asp Asn Phe Lys Glu Tyr Leu Arg His Gly Glu Glu Tyr 355 360 365 Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu Thr Ala Asp 370 375 380 Val Met Thr Tyr Ile His Ser Met Asn Pro Ser Ile Leu Glu Asp Trp 385 390 395 400 Asn Phe Gly Leu Thr Pro Pro Pro Ser Gly Thr Leu Glu Asp Thr Tyr 405 410 415 Arg Tyr Val Thr Ser Gln Ala Val Thr Cys Gln Lys Pro Ser Ala Pro 420 425 430 Lys Pro Lys Asp Asp Pro Leu Lys Asn Tyr Thr Phe Trp Glu Val Asp 435 440 445 Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro Leu Gly Arg 450 455 460 Lys Phe Leu Leu Gln Ala Gly Leu 465 470 <![CDATA[<210> 70]]> <![CDATA[<211> 26]]> <![CDATA[<212> PRT]]> <![CDATA[<213> ]]>智人 <![CDATA[<220> ]]> <![CDATA[<223> 3502 HPV 33型L1蛋白的474-499胺基酸序列]]> <![CDATA[<400> 70]]> Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr 1 5 10 15 Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 20 25 <![CDATA[<210> 71]]> <![CDATA[<211> 498]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3503 嵌合的HPV 35型L1蛋白的胺基酸序列]]> <![CDATA[<400]]>> 71]]> <br/><![CDATA[Met Ser Leu Trp Arg Ser Asn Glu Ala Thr Val Tyr Leu Pro Pro Val 1 5 10 15 Ser Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Thr Arg Thr Asn 20 25 30 Ile Tyr Tyr His Ala Gly Ser Ser Arg Leu Leu Ala Val Gly His Pro 35 40 45 Tyr Tyr Ala Ile Lys Lys Gln Asp Ser Asn Lys Ile Ala Val Pro Lys 50 55 60 Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Val Lys Leu Pro Asp Pro 65 70 75 80 Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asp Pro Ala Ser Gln 85 90 95 Arg Leu Val Trp Ala Cys Thr Gly Val Glu Val Gly Arg Gly Gln Pro 100 105 110 Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys Leu Asp Asp 115 120 125 Thr Glu Asn Ser Asn Lys Tyr Val Gly Asn Ser Gly Thr Asp Asn Arg 130 135 140 Glu Cys Ile Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Ile Gly 145 150 155 160 Cys Arg Pro Pro Ile Gly Glu His Trp Gly Lys Gly Thr Pro Cys Asn 165 170 175 Ala Asn Gln Val Lys Ala Gly Glu Cys Pro Pro Leu Glu Leu Leu Asn 180 185 190 Thr Val Leu Gln Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala Met 195 200 205 Asp Phe Thr Thr Leu Gln Ala Asn Lys Ser Asp Val Pro Leu Asp Ile 210 215 220 Cys Ser Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Lys Met Val Ser Glu 225 230 235 240 Pro Tyr Gly Asp Met Leu Phe Phe Tyr Leu Arg Arg Glu Gln Met Phe 245 250 255 Val Arg His Leu Phe Asn Arg Ala Gly Thr Val Gly Glu Thr Val Pro 260 265 270 Ala Asp Leu Tyr Ile Lys Gly Thr Thr Gly Thr Leu Pro Ser Thr Ser 275 280 285 Tyr Phe Pro Thr Pro Ser Gly Ser Met Val Thr Ser Asp Ala Gln Ile 290 295 300 Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn Asn Gly 305 310 315 320 Ile Cys Trp Ser Asn Gln Leu Phe Val Thr Val Val Asp Thr Thr Arg 325 330 335 Ser Thr Asn Met Ser Val Cys Ser Ala Val Ser Ser Ser Asp Ser Thr 340 345 350 Tyr Lys Asn Asp Asn Phe Lys Glu Tyr Leu Arg His Gly Glu Glu Tyr 355 360 365 Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu Thr Ala Asp 370 375 380 Val Met Thr Tyr Ile His Ser Met Asn Pro Ser Ile Leu Glu Asp Trp 385 390 395 400 Asn Phe Gly Leu Thr Pro Pro Pro Ser Gly Thr Leu Glu Asp Thr Tyr 405 410 415 Arg Tyr Val Thr Ser Gln Ala Val Thr Cys Gln Lys Pro Ser Ala Pro 420 425 430 Lys Pro Lys Asp Asp Pro Leu Lys Asn Tyr Thr Phe Trp Glu Val Asp 435 440 445 Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro Leu Gly Arg 450 455 460 Lys Phe Leu Leu Gln Ala Gly Leu Lys Ala Lys Pro Lys Leu Lys Arg 465 470 475 480 Ala Ala Pro Thr Ser Thr Arg Thr Ser Ser Ala Lys Arg Lys Lys Val 485 490 495 Lys Lys <![CDATA[<210> 72]]> <![CDATA[<211> 1498]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3504 嵌合的HPV 35型L1蛋白的核苷酸序列]]> <![CDATA[<400> 72]]> atgagtctgt ggaggagcaa tgaggctaca gtctacctgc ctcctgtgtc tgtgagcaag 60 gtggtgagca cagatgaata tgtgaccagg accaacatct actaccatgc tggctccagc 120 agactgctgg ctgtgggaca cccatactat gccatcaaga agcaggacag caacaagatt 180 gctgtgccaa aggtgtctgg actccaatac agggtgttca gggtgaaact gcctgaccca 240 aacaagtttg gctttcctga cacctccttc tatgaccctg ccagccagag actggtgtgg 300 gcttgtactg gagtggaggt gggcagggga caaccactgg gagtgggcat ctctggacac 360 ccactgctga acaaactgga tgacacagag aacagcaaca aatatgtggg caactctggc 420 acagacaaca gggagtgtat cagtatggac tacaagcaga cccaactttg tctgattggc 480 tgtagacctc caattggaga acactggggc aagggcacac catgtaatgc caaccaggtg 540 aaggctggag agtgtcctcc attggaactg ctgaacacag tgctccaaga tggagatatg 600 gtggacacag gctttggagc tatggacttc accaccctcc aagccaacaa gtctgatgtg 660 ccactggaca tctgttccag catctgtaaa taccctgact acctgaaaat ggtgtctgaa 720 ccatatggag atatgctgtt cttctacctg aggagggaac agatgtttgt gagacacctg 780 ttcaacaggg ctggcacagt gggagagaca gtgcctgctg acctctacat caagggcacc 840 acaggcaccc tgccaagcac ctcctacttt ccaacaccat ctggcagtat ggtgacctct 900 gatgcccaga ttttcaacaa gccatactgg ctccaaaggg ctcaaggaca caacaatggc 960 atctgttgga gcaaccaact ttttgtgaca gtggtggaca ccaccaggag caccaatatg 1020 agtgtgtgtt ctgctgtgtc ctcctctgac agcacctaca agaatgacaa cttcaaggaa 1080 tacctgagac atggagagga atatgacctc caattcatct tccaactttg taagattacc 1140 ctgacagcag atgtgatgac ctacatccac agtatgaacc caagcatctt ggaggactgg 1200 aactttggac tgacacctcc tccatctggc accttggagg acacctacag atatgtgacc 1260 agccaggctg tgacttgtca gaagccatct gccccaaagc caaaggatga cccactgaaa 1320 aactacacct tctgggaggt ggacctgaaa gagaagttct ctgctgacct ggaccagttt 1380 ccactgggca ggaagttcct gctccaagca ggactgaaag ccaagccaaa actgaaaagg 1440 gctgccccaa ccagcaccag gacctcctct gccaagagga agaaggtgaa gaagtaaa 1498 <![CDATA[<210> 73]]> <![CDATA[<211> 1528]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3505 合成的HPV35L1基因]]> <![CDATA[<400> 73]]> ctgggtacca tgagtctgtg gaggagcaat gaggctacag tctacctgcc tcctgtgtct 60 gtgagcaagg tggtgagcac agatgaatat gtgaccagga ccaacatcta ctaccatgct 120 ggctccagca gactgctggc tgtgggacac ccatactatg ccatcaagaa gcaggacagc 180 aacaagattg ctgtgccaaa ggtgtctgga ctccaataca gggtgttcag ggtgaaactg 240 cctgacccaa acaagtttgg ctttcctgac acctccttct atgaccctgc cagccagaga 300 ctggtgtggg cttgtactgg agtggaggtg ggcaggggac aaccactggg agtgggcatc 360 tctggacacc cactgctgaa caaactggat gacacagaga acagcaacaa atatgtgggc 420 aactctggca cagacaacag ggagtgtatc agtatggact acaagcagac ccaactttgt 480 ctgattggct gtagacctcc aattggagaa cactggggca agggcacacc atgtaatgcc 540 aaccaggtga aggctggaga gtgtcctcca ttggaactgc tgaacacagt gctccaagat 600 ggagatatgg tggacacagg ctttggagct atggacttca ccaccctcca agccaacaag 660 tctgatgtgc cactggacat ctgttccagc atctgtaaat accctgacta cctgaaaatg 720 gtgtctgaac catatggaga tatgctgttc ttctacctga ggagggaaca gatgtttgtg 780 agacacctgt tcaacagggc tggcacagtg ggagagacag tgcctgctga cctctacatc 840 aagggcacca caggcaccct gccaagcacc tcctactttc caacaccatc tggcagtatg 900 gtgacctctg atgcccagat tttcaacaag ccatactggc tccaaagggc tcaaggacac 960 aacaatggca tctgttggag caaccaactt tttgtgacag tggtggacac caccaggagc 1020 accaatatga gtgtgtgttc tgctgtgtcc tcctctgaca gcacctacaa gaatgacaac 1080 ttcaaggaat acctgagaca tggagaggaa tatgacctcc aattcatctt ccaactttgt 1140 aagattaccc tgacagcaga tgtgatgacc tacatccaca gtatgaaccc aagcatcttg 1200 gaggactgga actttggact gacacctcct ccatctggca ccttggagga cacctacaga 1260 tatgtgacca gccaggctgt gacttgtcag aagccatctg ccccaaagcc aaaggatgac 1320 ccactgaaaa actacacctt ctgggaggtg gacctgaaag agaagttctc tgctgacctg 1380 gaccagtttc cactgggcag gaagttcctg ctccaagcag gactgaaagc cagaccaaac 1440 ttcagactgg gcaagagggc tgcccctgcc agcaccagca agaagtccag caccaagagg 1500 aggaaggtga agagctaaac tcgagctc 1528 <![CDATA[<210> 74]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3506 合成的HPV33L1基因]]> <![CDATA[<400> 74]]> ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct 120 ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat 180 gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga 240 ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag 300 agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc 360 atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct 420 ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt 480 tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc 540 aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat 600 ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag 660 tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg 720 acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg 780 agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc 840 aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc 900 agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc 1020 aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag 1080 aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt 1140 tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc 1200 ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac 1260 aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag 1320 gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac 1380 ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca 1440 aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg 1500 aagaagtaaa ctcgagctc 1519 <![CDATA[<210> 75]]> <![CDATA[<211> 34]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3507 HPV35L1 F1]]> <![CDATA[<400> 75]]> cttggtacca tgagtctgtg gaggagcaat gagg 34 <![CDATA[<210> 76]]> <![CDATA[<211> 36]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3508 HPV35L1 R1]]> <![CDATA[<400> 76]]> gcttggcttt cagtcctgct tggagcagga acttcc 36 <![CDATA[<210> 77]]> <![CDATA[<211> 1435]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3509 HPV35L1擴增序列1]]> <![CDATA[<400> 77]]> cttggtacca tgagtctgtg gaggagcaat gaggctacag tctacctgcc tcctgtgtct 60 gtgagcaagg tggtgagcac agatgaatat gtgaccagga ccaacatcta ctaccatgct 120 ggctccagca gactgctggc tgtgggacac ccatactatg ccatcaagaa gcaggacagc 180 aacaagattg ctgtgccaaa ggtgtctgga ctccaataca gggtgttcag ggtgaaactg 240 cctgacccaa acaagtttgg ctttcctgac acctccttct atgaccctgc cagccagaga 300 ctggtgtggg cttgtactgg agtggaggtg ggcaggggac aaccactggg agtgggcatc 360 tctggacacc cactgctgaa caaactggat gacacagaga acagcaacaa atatgtgggc 420 aactctggca cagacaacag ggagtgtatc agtatggact acaagcagac ccaactttgt 480 ctgattggct gtagacctcc aattggagaa cactggggca agggcacacc atgtaatgcc 540 aaccaggtga aggctggaga gtgtcctcca ttggaactgc tgaacacagt gctccaagat 600 ggagatatgg tggacacagg ctttggagct atggacttca ccaccctcca agccaacaag 660 tctgatgtgc cactggacat ctgttccagc atctgtaaat accctgacta cctgaaaatg 720 gtgtctgaac catatggaga tatgctgttc ttctacctga ggagggaaca gatgtttgtg 780 agacacctgt tcaacagggc tggcacagtg ggagagacag tgcctgctga cctctacatc 840 aagggcacca caggcaccct gccaagcacc tcctactttc caacaccatc tggcagtatg 900 gtgacctctg atgcccagat tttcaacaag ccatactggc tccaaagggc tcaaggacac 960 aacaatggca tctgttggag caaccaactt tttgtgacag tggtggacac caccaggagc 1020 accaatatga gtgtgtgttc tgctgtgtcc tcctctgaca gcacctacaa gaatgacaac 1080 ttcaaggaat acctgagaca tggagaggaa tatgacctcc aattcatctt ccaactttgt 1140 aagattaccc tgacagcaga tgtgatgacc tacatccaca gtatgaaccc aagcatcttg 1200 gaggactgga actttggact gacacctcct ccatctggca ccttggagga cacctacaga 1260 tatgtgacca gccaggctgt gacttgtcag aagccatctg ccccaaagcc aaaggatgac 1320 ccactgaaaa actacacctt ctgggaggtg gacctgaaag agaagttctc tgctgacctg 1380 gaccagtttc cactgggcag gaagttcctg ctccaagcag gactgaaagc caagc 1435 <![CDATA[<210> 78]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<22]]>3> 3510 HPV35L1 F2]]> <br/> <br/><![CDATA[<400> 78]]> <br/><![CDATA[agcaggactg aaagccaagc caaaactgaa aaggg 35 <![CDATA[<210> 79]]> <![CDATA[<211> 36]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3511 HPV35L1 R2]]> <![CDATA[<400> 79]]> ctgtctagat ttacttcttc accttcttcc tcttgg 36 <![CDATA[<210> 80]]> <![CDATA[<211> 101]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3512 HPV35L1擴增序列2]]> <![CDATA[<400> 80]]> agcaggactg aaagccaagc caaaactgaa aagggctgcc ccaaccagca ccaggacctc 60 ctctgccaag aggaagaagg tgaagaagta aatctagaca g 101 <![CDATA[<210> 81]]> <![CDATA[<211> 38]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 3513 HPV 59型L1蛋白的471-508胺基酸序列]]> <![CDATA[<400> 81]]> Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 1 5 10 15 Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 20 25 30 Arg Lys Ser Ser Arg Lys 35 <![CDATA[<210> 82]]> <![CDATA[<211> 469]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 3901 HPV 39型L1蛋白的1-469胺基酸序列]]> <![CDATA[<400> 82]]> Met Ala Met Trp Arg Ser Ser Asp Ser Met Val Tyr Leu Pro Pro Pro 1 5 10 15 Ser Val Ala Lys Val Val Asn Thr Asp Asp Tyr Val Thr Arg Thr Gly 20 25 30 Ile Tyr Tyr Tyr Ala Gly Ser Ser Arg Leu Leu Thr Val Gly His Pro 35 40 45 Tyr Phe Lys Val Gly Met Asn Gly Gly Arg Lys Gln Asp Ile Pro Lys 50 55 60 Val Ser Ala Tyr Gln Tyr Arg Val Phe Arg Val Thr Leu Pro Asp Pro 65 70 75 80 Asn Lys Phe Ser Ile Pro Asp Ala Ser Leu Tyr Asn Pro Glu Thr Gln 85 90 95 Arg Leu Val Trp Ala Cys Val Gly Val Glu Val Gly Arg Gly Gln Pro 100 105 110 Leu Gly Val Gly Ile Ser Gly His Pro Leu Tyr Asn Arg Gln Asp Asp 115 120 125 Thr Glu Asn Ser Pro Phe Ser Ser Thr Thr Asn Lys Asp Ser Arg Asp 130 135 140 Asn Val Ser Val Asp Tyr Lys Gln Thr Gln Leu Cys Ile Ile Gly Cys 145 150 155 160 Val Pro Ala Ile Gly Glu His Trp Gly Lys Gly Lys Ala Cys Lys Pro 165 170 175 Asn Asn Val Ser Thr Gly Asp Cys Pro Pro Leu Glu Leu Val Asn Thr 180 185 190 Pro Ile Glu Asp Gly Asp Met Ile Asp Thr Gly Tyr Gly Ala Met Asp 195 200 205 Phe Gly Ala Leu Gln Glu Thr Lys Ser Glu Val Pro Leu Asp Ile Cys 210 215 220 Gln Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Gln Met Ser Ala Asp Val 225 230 235 240 Tyr Gly Asp Ser Met Phe Phe Cys Leu Arg Arg Glu Gln Leu Phe Ala 245 250 255 Arg His Phe Trp Asn Arg Gly Gly Met Val Gly Asp Ala Ile Pro Ala 260 265 270 Gln Leu Tyr Ile Lys Gly Thr Asp Ile Arg Ala Asn Pro Gly Ser Ser 275 280 285 Val Tyr Cys Pro Ser Pro Ser Gly Ser Met Val Thr Ser Asp Ser Gln 290 295 300 Leu Phe Asn Lys Pro Tyr Trp Leu His Lys Ala Gln Gly His Asn Asn 305 310 315 320 Gly Ile Cys Trp His Asn Gln Leu Phe Leu Thr Val Val Asp Thr Thr 325 330 335 Arg Ser Thr Asn Phe Thr Leu Ser Thr Ser Ile Glu Ser Ser Ile Pro 340 345 350 Ser Thr Tyr Asp Pro Ser Lys Phe Lys Glu Tyr Thr Arg His Val Glu 355 360 365 Glu Tyr Asp Leu Gln Phe Ile Phe Gln Leu Cys Thr Val Thr Leu Thr 370 375 380 Thr Asp Val Met Ser Tyr Ile His Thr Met Asn Ser Ser Ile Leu Asp 385 390 395 400 Asn Trp Asn Phe Ala Val Ala Pro Pro Pro Ser Ala Ser Leu Val Asp 405 410 415 Thr Tyr Arg Tyr Leu Gln Ser Ala Ala Ile Thr Cys Gln Lys Asp Ala 420 425 430 Pro Ala Pro Glu Lys Lys Asp Pro Tyr Asp Gly Leu Lys Phe Trp Asn 435 440 445 Val Asp Leu Arg Glu Lys Phe Ser Leu Glu Leu Asp Gln Phe Pro Leu 450 455 460 Gly Arg Lys Phe Leu 465 <![CDATA[<210> 83]]> <![CDATA[<211> 38]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 3902 HPV 59型L1蛋白的471-508胺基酸序列]]> <![CDATA[<400> 83]]> Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 1 5 10 15 Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 20 25 30 Arg Lys Ser Ser Arg Lys 35 <![CDATA[<210> 84]]> <![CDATA[<211> 507]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3903 嵌合的HPV 39型L1蛋白的胺基酸序列]]> <![CDATA[<400> 84]]> Met Ala Met Trp Arg Ser Ser Asp Ser Met Val Tyr Leu Pro Pro Pro 1 5 10 15 Ser Val Ala Lys Val Val Asn Thr Asp Asp Tyr Val Thr Arg Thr Gly 20 25 30 Ile Tyr Tyr Tyr Ala Gly Ser Ser Arg Leu Leu Thr Val Gly His Pro 35 40 45 Tyr Phe Lys Val Gly Met Asn Gly Gly Arg Lys Gln Asp Ile Pro Lys 50 55 60 Val Ser Ala Tyr Gln Tyr Arg Val Phe Arg Val Thr Leu Pro Asp Pro 65 70 75 80 Asn Lys Phe Ser Ile Pro Asp Ala Ser Leu Tyr Asn Pro Glu Thr Gln 85 90 95 Arg Leu Val Trp Ala Cys Val Gly Val Glu Val Gly Arg Gly Gln Pro 100 105 110 Leu Gly Val Gly Ile Ser Gly His Pro Leu Tyr Asn Arg Gln Asp Asp 115 120 125 Thr Glu Asn Ser Pro Phe Ser Ser Thr Thr Asn Lys Asp Ser Arg Asp 130 135 140 Asn Val Ser Val Asp Tyr Lys Gln Thr Gln Leu Cys Ile Ile Gly Cys 145 150 155 160 Val Pro Ala Ile Gly Glu His Trp Gly Lys Gly Lys Ala Cys Lys Pro 165 170 175 Asn Asn Val Ser Thr Gly Asp Cys Pro Pro Leu Glu Leu Val Asn Thr 180 185 190 Pro Ile Glu Asp Gly Asp Met Ile Asp Thr Gly Tyr Gly Ala Met Asp 195 200 205 Phe Gly Ala Leu Gln Glu Thr Lys Ser Glu Val Pro Leu Asp Ile Cys 210 215 220 Gln Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Gln Met Ser Ala Asp Val 225 230 235 240 Tyr Gly Asp Ser Met Phe Phe Cys Leu Arg Arg Glu Gln Leu Phe Ala 245 250 255 Arg His Phe Trp Asn Arg Gly Gly Met Val Gly Asp Ala Ile Pro Ala 260 265 270 Gln Leu Tyr Ile Lys Gly Thr Asp Ile Arg Ala Asn Pro Gly Ser Ser 275 280 285 Val Tyr Cys Pro Ser Pro Ser Gly Ser Met Val Thr Ser Asp Ser Gln 290 295 300 Leu Phe Asn Lys Pro Tyr Trp Leu His Lys Ala Gln Gly His Asn Asn 305 310 315 320 Gly Ile Cys Trp His Asn Gln Leu Phe Leu Thr Val Val Asp Thr Thr 325 330 335 Arg Ser Thr Asn Phe Thr Leu Ser Thr Ser Ile Glu Ser Ser Ile Pro 340 345 350 Ser Thr Tyr Asp Pro Ser Lys Phe Lys Glu Tyr Thr Arg His Val Glu 355 360 365 Glu Tyr Asp Leu Gln Phe Ile Phe Gln Leu Cys Thr Val Thr Leu Thr 370 375 380 Thr Asp Val Met Ser Tyr Ile His Thr Met Asn Ser Ser Ile Leu Asp 385 390 395 400 Asn Trp Asn Phe Ala Val Ala Pro Pro Pro Ser Ala Ser Leu Val Asp 405 410 415 Thr Tyr Arg Tyr Leu Gln Ser Ala Ala Ile Thr Cys Gln Lys Asp Ala 420 425 430 Pro Ala Pro Glu Lys Lys Asp Pro Tyr Asp Gly Leu Lys Phe Trp Asn 435 440 445 Val Asp Leu Arg Glu Lys Phe Ser Leu Glu Leu Asp Gln Phe Pro Leu 450 455 460 Gly Arg Lys Phe Leu Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile 465 470 475 480 Gly Pro Arg Lys Arg Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro 485 490 495 Lys Arg Val Lys Arg Arg Lys Ser Ser Arg Lys 500 505 <![CDATA[<210> 85]]> <![CDATA[<211> 1525]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3904 嵌合的HPV 39型L1蛋白的核苷酸序列]]> <![CDATA[<400> 85]]> atggctatgt ggaggtcctc tgacagtatg gtctacctgc ctcctccatc tgtggctaag 60 gtggtgaaca cagatgacta tgtgaccagg acaggcatct actactatgc tggctccagc 120 agactgctga cagtgggaca cccatacttc aaggtgggga tgaatggagg caggaagcag 180 gacatcccaa aggtgtctgc ctaccaatac agggtgttca gggtgaccct gcctgaccca 240 aacaagttca gcatccctga tgcctccctc tacaaccctg agacccagag actggtgtgg 300 gcttgtgtgg gagtggaggt gggcagggga caaccactgg gagtgggcat ctctggacac 360 ccactctaca acagacagga tgacacagag aacagcccat tctccagcac caccaacaag 420 gacagcaggg acaatgtgtc tgtggactac aagcagaccc aactttgtat cattggctgt 480 gtgcctgcca ttggagaaca ctggggcaag ggcaaggctt gtaagccaaa caatgtgagc 540 acaggagact gtcctccatt ggaactggtg aacacaccaa ttgaggatgg agatatgatt 600 gacacaggct atggagctat ggactttgga gccctccaag agaccaagtc tgaggtgcca 660 ctggacatct gtcagagcat ctgtaaatac cctgactacc tccaaatgag tgctgatgtc 720 tatggagaca gtatgttctt ctgtctgagg agggaacaac tttttgccag acacttctgg 780 aacaggggag ggatggtggg agatgccatc cctgcccaac tctacatcaa gggcacagac 840 atcagggcta accctggctc ctctgtctac tgtccaagcc catctggcag tatggtgacc 900 tctgacagcc aacttttcaa caagccatac tggctgcaca aggctcaagg acacaacaat 960 ggcatctgtt ggcacaacca acttttcctg acagtggtgg acaccaccag gagcaccaac 1020 ttcaccctga gcaccagcat tgagtccagc atcccaagca cctatgaccc aagcaagttc 1080 aaggaataca ccaggcatgt ggaggaatat gacctccaat tcatcttcca actttgtact 1140 gtgaccctga ccacagatgt gatgagttac atccacacaa tgaactccag catcctggac 1200 aactggaact ttgctgtggc tcctcctcca tctgcctccc tggtggacac ctacagatac 1260 ctccaatctg ctgccatcac ttgtcagaag gatgcccctg cccctgagaa gaaggaccca 1320 tatgatggac tgaagttctg gaatgtggac ctgagggaga agttctcctt ggaactggac 1380 cagtttccac tgggcaggaa gttcctgctc caacttggag ccagaccaaa gccaaccatt 1440 ggaccaagga agagggctgc ccctgcccca accagcacac caagcccaaa gagggtgaag 1500 aggaggaagt ccagcaggaa gtaaa 1525 <![CDATA[<210> 86]]> <![CDATA[<211> 1537]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3905 合成的HPV39L1基因]]> <![CDATA[<400> 86]]> ctgggtacca tggctatgtg gaggtcctct gacagtatgg tctacctgcc tcctccatct 60 gtggctaagg tggtgaacac agatgactat gtgaccagga caggcatcta ctactatgct 120 ggctccagca gactgctgac agtgggacac ccatacttca aggtggggat gaatggaggc 180 aggaagcagg acatcccaaa ggtgtctgcc taccaataca gggtgttcag ggtgaccctg 240 cctgacccaa acaagttcag catccctgat gcctccctct acaaccctga gacccagaga 300 ctggtgtggg cttgtgtggg agtggaggtg ggcaggggac aaccactggg agtgggcatc 360 tctggacacc cactctacaa cagacaggat gacacagaga acagcccatt ctccagcacc 420 accaacaagg acagcaggga caatgtgtct gtggactaca agcagaccca actttgtatc 480 attggctgtg tgcctgccat tggagaacac tggggcaagg gcaaggcttg taagccaaac 540 aatgtgagca caggagactg tcctccattg gaactggtga acacaccaat tgaggatgga 600 gatatgattg acacaggcta tggagctatg gactttggag ccctccaaga gaccaagtct 660 gaggtgccac tggacatctg tcagagcatc tgtaaatacc ctgactacct ccaaatgagt 720 gctgatgtct atggagacag tatgttcttc tgtctgagga gggaacaact ttttgccaga 780 cacttctgga acaggggagg gatggtggga gatgccatcc ctgcccaact ctacatcaag 840 ggcacagaca tcagggctaa ccctggctcc tctgtctact gtccaagccc atctggcagt 900 atggtgacct ctgacagcca acttttcaac aagccatact ggctgcacaa ggctcaagga 960 cacaacaatg gcatctgttg gcacaaccaa cttttcctga cagtggtgga caccaccagg 1020 agcaccaact tcaccctgag caccagcatt gagtccagca tcccaagcac ctatgaccca 1080 agcaagttca aggaatacac caggcatgtg gaggaatatg acctccaatt catcttccaa 1140 ctttgtactg tgaccctgac cacagatgtg atgagttaca tccacacaat gaactccagc 1200 atcctggaca actggaactt tgctgtggct cctcctccat ctgcctccct ggtggacacc 1260 tacagatacc tccaatctgc tgccatcact tgtcagaagg atgcccctgc ccctgagaag 1320 aaggacccat atgatggact gaagttctgg aatgtggacc tgagggagaa gttctccttg 1380 gaactggacc agtttccact gggcaggaag ttcctgctcc aagccagggt gaggaggaga 1440 ccaaccattg gaccaaggaa gagacctgct gccagcacct cctcctcctc tgccaccaaa 1500 cacaagagga agagggtgag caagtaaact cgagctc 1537 <![CDATA[<210> 87]]> <![CDATA[<211> 1546]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3906 合成的HPV59L1基因]]> <![CDATA[<400> 87]]> ctgggtacca tggctctgtg gaggtcctct gacaacaagg tctacctgcc tcctccatct 60 gtggctaagg tggtgagcac agatgaatat gtgaccagga ccagcatctt ctaccatgct 120 ggctccagca gactgctgac agtgggacac ccatacttca aggtgccaaa gggaggcaat 180 ggcagacagg atgtgccaaa ggtgtctgcc taccaataca gggtgttcag ggtgaaactg 240 cctgacccaa acaagtttgg actgcctgac aacacagtct atgacccaaa cagccagaga 300 ctggtgtggg cttgtgtggg agtggagatt ggcaggggac aaccactggg agtgggactg 360 tctggacacc cactctacaa caaactggat gacacagaga actctcatgt ggcatctgct 420 gtggacacca aggacaccag ggacaatgtg tctgtggact acaagcagac ccaactttgt 480 atcattggct gtgtgcctgc cattggagaa cactggacca agggcacagc ctgtaagcca 540 accacagtgg tccagggaga ctgtcctcca ttggaactga taaacacacc aattgaggat 600 ggagatatgg tggacacagg ctatggagct atggacttca aactgctcca agacaacaag 660 tctgaggtgc cactggacat ctgtcagagc atctgtaaat accctgacta cctccaaatg 720 agtgctgatg cctatggaga cagtatgttc ttctgtctga ggagggaaca ggtgtttgcc 780 agacacttct ggaacaggtc tggcacaatg ggagaccaac ttcctgagtc cctctacatc 840 aagggcacag acatcagggc taaccctggc tcctacctct acagcccaag cccatctggc 900 tctgtggtga cctctgacag ccaacttttc aacaagccat actggctgca caaggctcaa 960 ggactgaaca atggcatctg ttggcacaac caacttttcc tgacagtggt ggacaccacc 1020 aggagcacca acctgtctgt gtgtgccagc accacctcca gcatcccaaa tgtctacaca 1080 ccaacctcct tcaaggaata tgccaggcat gtggaggagt ttgacctcca attcatcttc 1140 caactttgta agattaccct gaccacagag gtgatgagtt acatccacaa tatgaacacc 1200 accatcttgg aggactggaa ctttggagtg acacctcctc caacagcctc cctggtggac 1260 acctacaggt ttgtccagtc tgctgctgtg acttgtcaga aggacacagc ccctcctgtg 1320 aagcaggacc catatgacaa actgaagttc tggcctgtgg acctgaaaga gaggttctct 1380 gctgacctgg accagtttcc actgggcagg aagttcctgc tccaacttgg agccagacca 1440 aagccaacca ttggaccaag gaagagggct gcccctgccc caaccagcac accaagccca 1500 aagagggtga agaggaggaa gtccagcagg aagtaaactc gagctc 1546 <![CDATA[<210> 88]]> <![CDATA[<211> 38]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3907 HPV39L1 F1]]> <![CDATA[<400> 88]]> cttggtacca tggctatgtg gaggtcctct gacagtat 38 <![CDATA[<210> 89]]> <![CDATA[<211> 38]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3908 HPV39L1 R1]]> <![CDATA[<400> 89]]> tccaagttgg agcaggaact tcctgcccag tggaaact 38 <![CDATA[<210> 90]]> <![CDATA[<211> 1428]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3909 HPV39L1擴增序列1]]> <![CDATA[<400> 90]]> cttggtacca tggctatgtg gaggtcctct gacagtatgg tctacctgcc tcctccatct 60 gtggctaagg tggtgaacac agatgactat gtgaccagga caggcatcta ctactatgct 120 ggctccagca gactgctgac agtgggacac ccatacttca aggtggggat gaatggaggc 180 aggaagcagg acatcccaaa ggtgtctgcc taccaataca gggtgttcag ggtgaccctg 240 cctgacccaa acaagttcag catccctgat gcctccctct acaaccctga gacccagaga 300 ctggtgtggg cttgtgtggg agtggaggtg ggcaggggac aaccactggg agtgggcatc 360 tctggacacc cactctacaa cagacaggat gacacagaga acagcccatt ctccagcacc 420 accaacaagg acagcaggga caatgtgtct gtggactaca agcagaccca actttgtatc 480 attggctgtg tgcctgccat tggagaacac tggggcaagg gcaaggcttg taagccaaac 540 aatgtgagca caggagactg tcctccattg gaactggtga acacaccaat tgaggatgga 600 gatatgattg acacaggcta tggagctatg gactttggag ccctccaaga gaccaagtct 660 gaggtgccac tggacatctg tcagagcatc tgtaaatacc ctgactacct ccaaatgagt 720 gctgatgtct atggagacag tatgttcttc tgtctgagga gggaacaact ttttgccaga 780 cacttctgga acaggggagg gatggtggga gatgccatcc ctgcccaact ctacatcaag 840 ggcacagaca tcagggctaa ccctggctcc tctgtctact gtccaagccc atctggcagt 900 atggtgacct ctgacagcca acttttcaac aagccatact ggctgcacaa ggctcaagga 960 cacaacaatg gcatctgttg gcacaaccaa cttttcctga cagtggtgga caccaccagg 1020 agcaccaact tcaccctgag caccagcatt gagtccagca tcccaagcac ctatgaccca 1080 agcaagttca aggaatacac caggcatgtg gaggaatatg acctccaatt catcttccaa 1140 ctttgtactg tgaccctgac cacagatgtg atgagttaca tccacacaat gaactccagc 1200 atcctggaca actggaactt tgctgtggct cctcctccat ctgcctccct ggtggacacc 1260 tacagatacc tccaatctgc tgccatcact tgtcagaagg atgcccctgc ccctgagaag 1320 aaggacccat atgatggact gaagttctgg aatgtggacc tgagggagaa gttctccttg 1380 gaactggacc agtttccact gggcaggaag ttcctgctcc aacttgga 1428 <![CDATA[<210> 91]]> <![CDATA[<211> 37]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3910 HPV39L1 F2]]> <![CDATA[<400> 91]]> aggaagttcc tgctccaact tggagccaga ccaaagc 37 <![CDATA[<210> 92]]> <![CDATA[<211> 40]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3911 HPV39L1 R2]]> <![CDATA[<400> 92]]> ctgtctagat ttacttcctg ctggacttcc tcctcttcac 40 <![CDATA[<210> 93]]> <![CDATA[<211> 139]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 3912 HPV39L1擴增序列2]]> <![CDATA[<400> 93]]> aggaagttcc tgctccaact tggagccaga ccaaagccaa ccattggacc aaggaagagg 60 gctgcccctg ccccaaccag cacaccaagc ccaaagaggg tgaagaggag gaagtccagc 120 aggaagtaaa tctagacag 139 <![CDATA[<210> 94]]> <![CDATA[<211> 26]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 3913 HPV 33型L1蛋白的474-499胺基酸序列]]> <![CDATA[<400> 94]]> Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr 1 5 10 15 Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 20 25 <![CDATA[<210> 95]]> <![CDATA[<211> 478]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 450]]>1 HPV 45型L1蛋白的1-478胺基酸序列 <![CDATA[<400> 95]]> Met Ala Leu Trp Arg Pro Ser Asp Ser Thr Val Tyr Leu Pro Pro Pro 1 5 10 15 Ser Val Ala Arg Val Val Asn Thr Asp Asp Tyr Val Ser Arg Thr Ser 20 25 30 Ile Phe Tyr His Ala Gly Ser Ser Arg Leu Leu Thr Val Gly Asn Pro 35 40 45 Tyr Phe Arg Val Val Pro Ser Gly Ala Gly Asn Lys Gln Ala Val Pro 50 55 60 Lys Val Ser Ala Tyr Gln Tyr Arg Val Phe Arg Val Ala Leu Pro Asp 65 70 75 80 Pro Asn Lys Phe Gly Leu Pro Asp Ser Thr Ile Tyr Asn Pro Glu Thr 85 90 95 Gln Arg Leu Val Trp Ala Cys Val Gly Met Glu Ile Gly Arg Gly Gln 100 105 110 Pro Leu Gly Ile Gly Leu Ser Gly His Pro Phe Tyr Asn Lys Leu Asp 115 120 125 Asp Thr Glu Ser Ala His Ala Ala Thr Ala Val Ile Thr Gln Asp Val 130 135 140 Arg Asp Asn Val Ser Val Asp Tyr Lys Gln Thr Gln Leu Cys Ile Leu 145 150 155 160 Gly Cys Val Pro Ala Ile Gly Glu His Trp Ala Lys Gly Thr Leu Cys 165 170 175 Lys Pro Ala Gln Leu Gln Pro Gly Asp Cys Pro Pro Leu Glu Leu Lys 180 185 190 Asn Thr Ile Ile Glu Asp Gly Asp Met Val Asp Thr Gly Tyr Gly Ala 195 200 205 Met Asp Phe Ser Thr Leu Gln Asp Thr Lys Cys Glu Val Pro Leu Asp 210 215 220 Ile Cys Gln Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Gln Met Ser Ala 225 230 235 240 Asp Pro Tyr Gly Asp Ser Met Phe Phe Cys Leu Arg Arg Glu Gln Leu 245 250 255 Phe Ala Arg His Phe Trp Asn Arg Ala Gly Val Met Gly Asp Thr Val 260 265 270 Pro Thr Asp Leu Tyr Ile Lys Gly Thr Ser Ala Asn Met Arg Glu Thr 275 280 285 Pro Gly Ser Cys Val Tyr Ser Pro Ser Pro Ser Gly Ser Ile Thr Thr 290 295 300 Ser Asp Ser Gln Leu Phe Asn Lys Pro Tyr Trp Leu His Lys Ala Gln 305 310 315 320 Gly His Asn Asn Gly Ile Cys Trp His Asn Gln Leu Phe Val Thr Val 325 330 335 Val Asp Thr Thr Arg Ser Thr Asn Leu Thr Leu Cys Ala Ser Thr Gln 340 345 350 Asn Pro Val Pro Asn Thr Tyr Asp Pro Thr Lys Phe Lys His Tyr Ser 355 360 365 Arg His Val Glu Glu Tyr Asp Leu Gln Phe Ile Phe Gln Leu Cys Thr 370 375 380 Ile Thr Leu Thr Ala Glu Val Met Ser Tyr Ile His Ser Met Asn Ser 385 390 395 400 Ser Ile Leu Glu Asn Trp Asn Phe Gly Val Pro Pro Pro Pro Thr Thr 405 410 415 Ser Leu Val Asp Thr Tyr Arg Phe Val Gln Ser Val Ala Val Thr Cys 420 425 430 Gln Lys Asp Thr Thr Pro Pro Glu Lys Gln Asp Pro Tyr Asp Lys Leu 435 440 445 Lys Phe Trp Thr Val Asp Leu Lys Glu Lys Phe Ser Ser Asp Leu Asp 450 455 460 Gln Tyr Pro Leu Gly Arg Lys Phe Leu Val Gln Ala Gly Leu 465 470 475 <![CDATA[<210> 96]]> <![CDATA[<211> 26]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 4502 HPV 33型L1蛋白的474-499胺基酸序列]]> <![CDATA[<400> 96]]> Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr 1 5 10 15 Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 20 25 <![CDATA[<210> 97]]> <![CDATA[<211> 504]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 4503 嵌合的HPV 45型L1蛋白的胺基酸序列]]> <![CDATA[<400> 97]]> Met Ala Leu Trp Arg Pro Ser Asp Ser Thr Val Tyr Leu Pro Pro Pro 1 5 10 15 Ser Val Ala Arg Val Val Asn Thr Asp Asp Tyr Val Ser Arg Thr Ser 20 25 30 Ile Phe Tyr His Ala Gly Ser Ser Arg Leu Leu Thr Val Gly Asn Pro 35 40 45 Tyr Phe Arg Val Val Pro Ser Gly Ala Gly Asn Lys Gln Ala Val Pro 50 55 60 Lys Val Ser Ala Tyr Gln Tyr Arg Val Phe Arg Val Ala Leu Pro Asp 65 70 75 80 Pro Asn Lys Phe Gly Leu Pro Asp Ser Thr Ile Tyr Asn Pro Glu Thr 85 90 95 Gln Arg Leu Val Trp Ala Cys Val Gly Met Glu Ile Gly Arg Gly Gln 100 105 110 Pro Leu Gly Ile Gly Leu Ser Gly His Pro Phe Tyr Asn Lys Leu Asp 115 120 125 Asp Thr Glu Ser Ala His Ala Ala Thr Ala Val Ile Thr Gln Asp Val 130 135 140 Arg Asp Asn Val Ser Val Asp Tyr Lys Gln Thr Gln Leu Cys Ile Leu 145 150 155 160 Gly Cys Val Pro Ala Ile Gly Glu His Trp Ala Lys Gly Thr Leu Cys 165 170 175 Lys Pro Ala Gln Leu Gln Pro Gly Asp Cys Pro Pro Leu Glu Leu Lys 180 185 190 Asn Thr Ile Ile Glu Asp Gly Asp Met Val Asp Thr Gly Tyr Gly Ala 195 200 205 Met Asp Phe Ser Thr Leu Gln Asp Thr Lys Cys Glu Val Pro Leu Asp 210 215 220 Ile Cys Gln Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Gln Met Ser Ala 225 230 235 240 Asp Pro Tyr Gly Asp Ser Met Phe Phe Cys Leu Arg Arg Glu Gln Leu 245 250 255 Phe Ala Arg His Phe Trp Asn Arg Ala Gly Val Met Gly Asp Thr Val 260 265 270 Pro Thr Asp Leu Tyr Ile Lys Gly Thr Ser Ala Asn Met Arg Glu Thr 275 280 285 Pro Gly Ser Cys Val Tyr Ser Pro Ser Pro Ser Gly Ser Ile Thr Thr 290 295 300 Ser Asp Ser Gln Leu Phe Asn Lys Pro Tyr Trp Leu His Lys Ala Gln 305 310 315 320 Gly His Asn Asn Gly Ile Cys Trp His Asn Gln Leu Phe Val Thr Val 325 330 335 Val Asp Thr Thr Arg Ser Thr Asn Leu Thr Leu Cys Ala Ser Thr Gln 340 345 350 Asn Pro Val Pro Asn Thr Tyr Asp Pro Thr Lys Phe Lys His Tyr Ser 355 360 365 Arg His Val Glu Glu Tyr Asp Leu Gln Phe Ile Phe Gln Leu Cys Thr 370 375 380 Ile Thr Leu Thr Ala Glu Val Met Ser Tyr Ile His Ser Met Asn Ser 385 390 395 400 Ser Ile Leu Glu Asn Trp Asn Phe Gly Val Pro Pro Pro Pro Thr Thr 405 410 415 Ser Leu Val Asp Thr Tyr Arg Phe Val Gln Ser Val Ala Val Thr Cys 420 425 430 Gln Lys Asp Thr Thr Pro Pro Glu Lys Gln Asp Pro Tyr Asp Lys Leu 435 440 445 Lys Phe Trp Thr Val Asp Leu Lys Glu Lys Phe Ser Ser Asp Leu Asp 450 455 460 Gln Tyr Pro Leu Gly Arg Lys Phe Leu Val Gln Ala Gly Leu Lys Ala 465 470 475 480 Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr Ser Ser 485 490 495 Ala Lys Arg Lys Lys Val Lys Lys 500 <![CDATA[<210> 98]]> <![CDATA[<211> 1516]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 4504]]> 嵌合的HPV 45型L1蛋白的核苷酸序列 <![CDATA[<400> 98]]> atggctctgt ggagaccatc tgacagcaca gtctacctgc ctcctccatc tgtggcaagg 60 gtggtgaaca cagatgacta tgtgagcagg accagcatct tctaccatgc tggctccagc 120 agactgctga cagtgggcaa cccatacttc agggtggtgc caagtggagc aggcaacaag 180 caggctgtgc caaaggtgtc tgcctaccaa tacagggtgt tcagggtggc tctgcctgac 240 ccaaacaagt ttggactgcc tgacagcacc atctacaacc ctgagaccca gagactggtg 300 tgggcttgtg tggggatgga gattggcagg ggacaaccac tgggcattgg actgtctgga 360 cacccattct acaacaaact ggatgacaca gagtctgccc atgctgccac agcagtgatt 420 acccaggatg tgagggacaa tgtgtctgtg gactacaagc agacccaact ttgtatcctg 480 ggctgtgtgc ctgccattgg agaacactgg gctaagggca ccctgtgtaa gcctgcccaa 540 ctccaacctg gagactgtcc tccattggaa ctgaaaaaca ccatcattga ggatggagat 600 atggtggaca caggctatgg agctatggac ttcagcaccc tccaagacac caagtgtgag 660 gtgccactgg acatctgtca gagcatctgt aaataccctg actacctcca aatgagtgct 720 gacccatatg gagacagtat gttcttctgt ctgaggaggg aacaactttt tgccagacac 780 ttctggaaca gggctggagt gatgggagac acagtgccaa cagacctcta catcaagggc 840 acctctgcca atatgaggga gacacctggc tcctgtgtct acagcccaag cccatctggc 900 agcatcacca cctctgacag ccaacttttc aacaagccat actggctgca caaggctcaa 960 ggacacaaca atggcatctg ttggcacaac caactttttg tgacagtggt ggacaccacc 1020 aggagcacca acctgaccct gtgtgccagc acccagaacc ctgtgccaaa cacctatgac 1080 ccaaccaagt tcaagcacta cagcaggcat gtggaggaat atgacctcca attcatcttc 1140 caactttgta ccatcaccct gacagcagag gtgatgagtt acatccacag tatgaactcc 1200 agcatcttgg agaactggaa ctttggagtg cctcctcctc caaccacctc cctggtggac 1260 acctacaggt ttgtccagtc tgtggctgtg acttgtcaga aggacaccac acctcctgag 1320 aagcaggacc catatgacaa actgaagttc tggacagtgg acctgaaaga gaagttctcc 1380 tctgacctgg accaataccc actgggcagg aagttcctgg tccaggctgg actgaaagcc 1440 aagccaaaac tgaaaagggc tgccccaacc agcaccagga cctcctctgc caagaggaag 1500 aaggtgaaga agtaaa 1516 <![CDATA[<210> 99]]> <![CDATA[<211> 1552]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 4505 合成的HPV45L1基因]]> <![CDATA[<400> 99]]> ctgggtacca tggctctgtg gagaccatct gacagcacag tctacctgcc tcctccatct 60 gtggcaaggg tggtgaacac agatgactat gtgagcagga ccagcatctt ctaccatgct 120 ggctccagca gactgctgac agtgggcaac ccatacttca gggtggtgcc aagtggagca 180 ggcaacaagc aggctgtgcc aaaggtgtct gcctaccaat acagggtgtt cagggtggct 240 ctgcctgacc caaacaagtt tggactgcct gacagcacca tctacaaccc tgagacccag 300 agactggtgt gggcttgtgt ggggatggag attggcaggg gacaaccact gggcattgga 360 ctgtctggac acccattcta caacaaactg gatgacacag agtctgccca tgctgccaca 420 gcagtgatta cccaggatgt gagggacaat gtgtctgtgg actacaagca gacccaactt 480 tgtatcctgg gctgtgtgcc tgccattgga gaacactggg ctaagggcac cctgtgtaag 540 cctgcccaac tccaacctgg agactgtcct ccattggaac tgaaaaacac catcattgag 600 gatggagata tggtggacac aggctatgga gctatggact tcagcaccct ccaagacacc 660 aagtgtgagg tgccactgga catctgtcag agcatctgta aataccctga ctacctccaa 720 atgagtgctg acccatatgg agacagtatg ttcttctgtc tgaggaggga acaacttttt 780 gccagacact tctggaacag ggctggagtg atgggagaca cagtgccaac agacctctac 840 atcaagggca cctctgccaa tatgagggag acacctggct cctgtgtcta cagcccaagc 900 ccatctggca gcatcaccac ctctgacagc caacttttca acaagccata ctggctgcac 960 aaggctcaag gacacaacaa tggcatctgt tggcacaacc aactttttgt gacagtggtg 1020 gacaccacca ggagcaccaa cctgaccctg tgtgccagca cccagaaccc tgtgccaaac 1080 acctatgacc caaccaagtt caagcactac agcaggcatg tggaggaata tgacctccaa 1140 ttcatcttcc aactttgtac catcaccctg acagcagagg tgatgagtta catccacagt 1200 atgaactcca gcatcttgga gaactggaac tttggagtgc ctcctcctcc aaccacctcc 1260 ctggtggaca cctacaggtt tgtccagtct gtggctgtga cttgtcagaa ggacaccaca 1320 cctcctgaga agcaggaccc atatgacaaa ctgaagttct ggacagtgga cctgaaagag 1380 aagttctcct ctgacctgga ccaataccca ctgggcagga agttcctggt ccaggctgga 1440 ctgaggagga gaccaaccat tggaccaagg aagagacctg ctgccagcac cagcacagcc 1500 agcagacctg ccaagagggt gaggattagg agcaagaagt aaactcgagc tc 1552 <![CDATA[<210> 100]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 4506 合成的HPV33L1基因]]> <![CDATA[<400> 100]]> ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct 120 ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat 180 gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga 240 ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag 300 agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc 360 atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct 420 ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt 480 tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc 540 aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat 600 ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag 660 tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg 720 acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg 780 agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc 840 aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc 900 agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc 1020 aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag 1080 aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt 1140 tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc 1200 ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac 1260 aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag 1320 gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac 1380 ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca 1440 aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg 1500 aagaagtaaa ctcgagctc 1519 <![CDATA[<210> 101]]> <![CDATA[<211> 33]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 4507 HPV45L1 F1]]> <![CDATA[<400> 101]]> cttggtacca tggctctgtg gagaccatct gac 33 <![CDATA[<210> 102]]> <![CDATA[<211> 32]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 4508 HPV45L1 R1]]> <![CDATA[<400> 102]]> gcttggcttt cagtccagcc tggaccagga ac 32 <![CDATA[<210> 103]]> <![CDATA[<211> 1453]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<22]]>0> ]]> <br/><![CDATA[<223> 4509 HPV45L1擴增序列1]]> <br/> <br/><![CDATA[<400> 103]]> <br/><![CDATA[cttggtacca tggctctgtg gagaccatct gacagcacag tctacctgcc tcctccatct 60 gtggcaaggg tggtgaacac agatgactat gtgagcagga ccagcatctt ctaccatgct 120 ggctccagca gactgctgac agtgggcaac ccatacttca gggtggtgcc aagtggagca 180 ggcaacaagc aggctgtgcc aaaggtgtct gcctaccaat acagggtgtt cagggtggct 240 ctgcctgacc caaacaagtt tggactgcct gacagcacca tctacaaccc tgagacccag 300 agactggtgt gggcttgtgt ggggatggag attggcaggg gacaaccact gggcattgga 360 ctgtctggac acccattcta caacaaactg gatgacacag agtctgccca tgctgccaca 420 gcagtgatta cccaggatgt gagggacaat gtgtctgtgg actacaagca gacccaactt 480 tgtatcctgg gctgtgtgcc tgccattgga gaacactggg ctaagggcac cctgtgtaag 540 cctgcccaac tccaacctgg agactgtcct ccattggaac tgaaaaacac catcattgag 600 gatggagata tggtggacac aggctatgga gctatggact tcagcaccct ccaagacacc 660 aagtgtgagg tgccactgga catctgtcag agcatctgta aataccctga ctacctccaa 720 atgagtgctg acccatatgg agacagtatg ttcttctgtc tgaggaggga acaacttttt 780 gccagacact tctggaacag ggctggagtg atgggagaca cagtgccaac agacctctac 840 atcaagggca cctctgccaa tatgagggag acacctggct cctgtgtcta cagcccaagc 900 ccatctggca gcatcaccac ctctgacagc caacttttca acaagccata ctggctgcac 960 aaggctcaag gacacaacaa tggcatctgt tggcacaacc aactttttgt gacagtggtg 1020 gacaccacca ggagcaccaa cctgaccctg tgtgccagca cccagaaccc tgtgccaaac 1080 acctatgacc caaccaagtt caagcactac agcaggcatg tggaggaata tgacctccaa 1140 ttcatcttcc aactttgtac catcaccctg acagcagagg tgatgagtta catccacagt 1200 atgaactcca gcatcttgga gaactggaac tttggagtgc ctcctcctcc aaccacctcc 1260 ctggtggaca cctacaggtt tgtccagtct gtggctgtga cttgtcagaa ggacaccaca 1320 cctcctgaga agcaggaccc atatgacaaa ctgaagttct ggacagtgga cctgaaagag 1380 aagttctcct ctgacctgga ccaataccca ctgggcagga agttcctggt ccaggctgga 1440 ctgaaagcca agc 1453 <![CDATA[<210> 104]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<2]]>20> ]]> <br/><![CDATA[<223> 4510 HPV45L1]]><![CDATA[ F2 <![CDATA[<400> 104]]> ggctggactg aaagccaagc caaaactgaa aaggg 35 <![CDATA[<210> 105]]> <![CDATA[<211> 37]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 4511 HPV45L1 R2]]> <![CDATA[<400> 105]]> ctgtctagat ttacttcttc accttcttcc tcttggc 37 <![CDATA[<210> 106]]> <![CDATA[<211> 101]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 4512 HPV45L1擴增序列2]]> <![CDATA[<400> 106]]> ggctggactg aaagccaagc caaaactgaa aagggctgcc ccaaccagca ccaggacctc 60 ctctgccaag aggaagaagg tgaagaagta aatctagaca g 101 <![CDATA[<210> 107]]> <![CDATA[<211> 38]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 4513 HPV 59型L1蛋白的471-508胺基酸序列]]> <![CDATA[<400> 107]]> Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 1 5 10 15 Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 20 25 30 Arg Lys Ser Ser Arg Lys 35 <![CDATA[<210> 108]]> <![CDATA[<211> 474]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5101 HPV 51型L1蛋白的1-474胺基酸序列]]> <![CDATA[<400> 108]]> Met Ala Leu Trp Arg Thr Asn Asp Ser Lys Val Tyr Leu Pro Pro Ala 1 5 10 15 Pro Val Ser Arg Ile Val Asn Thr Glu Glu Tyr Ile Thr Arg Thr Gly 20 25 30 Ile Tyr Tyr Tyr Ala Gly Ser Ser Arg Leu Ile Thr Leu Gly His Pro 35 40 45 Tyr Phe Pro Ile Pro Lys Thr Ser Thr Arg Ala Ala Ile Pro Lys Val 50 55 60 Ser Ala Phe Gln Tyr Arg Val Phe Arg Val Gln Leu Pro Asp Pro Asn 65 70 75 80 Lys Phe Gly Leu Pro Asp Pro Asn Leu Tyr Asn Pro Asp Thr Asp Arg 85 90 95 Leu Val Trp Gly Cys Val Gly Val Glu Val Gly Arg Gly Gln Pro Leu 100 105 110 Gly Val Gly Leu Ser Gly His Pro Leu Phe Asn Lys Tyr Asp Asp Thr 115 120 125 Glu Asn Ser Arg Ile Ala Asn Gly Asn Ala Gln Gln Asp Val Arg Asp 130 135 140 Asn Thr Ser Val Asp Asn Lys Gln Thr Gln Leu Cys Ile Ile Gly Cys 145 150 155 160 Ala Pro Pro Ile Gly Glu His Trp Gly Ile Gly Thr Thr Cys Lys Asn 165 170 175 Thr Pro Val Pro Pro Gly Asp Cys Pro Pro Leu Glu Leu Val Ser Ser 180 185 190 Val Ile Gln Asp Gly Asp Met Ile Asp Thr Gly Phe Gly Ala Met Asp 195 200 205 Phe Ala Ala Leu Gln Ala Thr Lys Ser Asp Val Pro Leu Asp Ile Ser 210 215 220 Gln Ser Val Cys Lys Tyr Pro Asp Tyr Leu Lys Met Ser Ala Asp Thr 225 230 235 240 Tyr Gly Asn Ser Met Phe Phe His Leu Arg Arg Glu Gln Ile Phe Ala 245 250 255 Arg His Tyr Tyr Asn Lys Leu Val Gly Val Gly Glu Asp Ile Pro Asn 260 265 270 Asp Tyr Tyr Ile Lys Gly Ser Gly Asn Gly Arg Asp Pro Ile Glu Ser 275 280 285 Tyr Ile Tyr Ser Ala Thr Pro Ser Gly Ser Met Ile Thr Ser Asp Ser 290 295 300 Gln Ile Phe Asn Lys Pro Tyr Trp Leu His Arg Ala Gln Gly His Asn 305 310 315 320 Asn Gly Ile Cys Trp Asn Asn Gln Leu Phe Ile Thr Cys Val Asp Thr 325 330 335 Thr Arg Ser Thr Asn Leu Thr Ile Ser Thr Ala Thr Ala Ala Val Ser 340 345 350 Pro Thr Phe Thr Pro Ser Asn Phe Lys Gln Tyr Ile Arg His Gly Glu 355 360 365 Glu Tyr Glu Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu Thr 370 375 380 Thr Glu Val Met Ala Tyr Leu His Thr Met Asp Pro Thr Ile Leu Glu 385 390 395 400 Gln Trp Asn Phe Gly Leu Thr Leu Pro Pro Ser Ala Ser Leu Glu Asp 405 410 415 Ala Tyr Arg Phe Val Arg Asn Ala Ala Thr Ser Cys Gln Lys Asp Thr 420 425 430 Pro Pro Gln Ala Lys Pro Asp Pro Leu Ala Lys Tyr Lys Phe Trp Asp 435 440 445 Val Asp Leu Lys Glu Arg Phe Ser Leu Asp Leu Asp Gln Phe Ala Leu 450 455 460 Gly Arg Lys Phe Leu Leu Gln Val Gly Val 465 470 <![CDATA[<210> 109]]> <![CDATA[<211> 26]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5102 HPV 33型]]>L1蛋白的474-499胺基酸序列 <![CDATA[<400> 109]]> Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr 1 5 10 15 Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 20 25 <![CDATA[<210> 110]]> <![CDATA[<211> 500]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5103 嵌合的HPV 51型L1蛋白的胺基酸序列]]> <![CDATA[<400> 110]]> Met Ala Leu Trp Arg Thr Asn Asp Ser Lys Val Tyr Leu Pro Pro Ala 1 5 10 15 Pro Val Ser Arg Ile Val Asn Thr Glu Glu Tyr Ile Thr Arg Thr Gly 20 25 30 Ile Tyr Tyr Tyr Ala Gly Ser Ser Arg Leu Ile Thr Leu Gly His Pro 35 40 45 Tyr Phe Pro Ile Pro Lys Thr Ser Thr Arg Ala Ala Ile Pro Lys Val 50 55 60 Ser Ala Phe Gln Tyr Arg Val Phe Arg Val Gln Leu Pro Asp Pro Asn 65 70 75 80 Lys Phe Gly Leu Pro Asp Pro Asn Leu Tyr Asn Pro Asp Thr Asp Arg 85 90 95 Leu Val Trp Gly Cys Val Gly Val Glu Val Gly Arg Gly Gln Pro Leu 100 105 110 Gly Val Gly Leu Ser Gly His Pro Leu Phe Asn Lys Tyr Asp Asp Thr 115 120 125 Glu Asn Ser Arg Ile Ala Asn Gly Asn Ala Gln Gln Asp Val Arg Asp 130 135 140 Asn Thr Ser Val Asp Asn Lys Gln Thr Gln Leu Cys Ile Ile Gly Cys 145 150 155 160 Ala Pro Pro Ile Gly Glu His Trp Gly Ile Gly Thr Thr Cys Lys Asn 165 170 175 Thr Pro Val Pro Pro Gly Asp Cys Pro Pro Leu Glu Leu Val Ser Ser 180 185 190 Val Ile Gln Asp Gly Asp Met Ile Asp Thr Gly Phe Gly Ala Met Asp 195 200 205 Phe Ala Ala Leu Gln Ala Thr Lys Ser Asp Val Pro Leu Asp Ile Ser 210 215 220 Gln Ser Val Cys Lys Tyr Pro Asp Tyr Leu Lys Met Ser Ala Asp Thr 225 230 235 240 Tyr Gly Asn Ser Met Phe Phe His Leu Arg Arg Glu Gln Ile Phe Ala 245 250 255 Arg His Tyr Tyr Asn Lys Leu Val Gly Val Gly Glu Asp Ile Pro Asn 260 265 270 Asp Tyr Tyr Ile Lys Gly Ser Gly Asn Gly Arg Asp Pro Ile Glu Ser 275 280 285 Tyr Ile Tyr Ser Ala Thr Pro Ser Gly Ser Met Ile Thr Ser Asp Ser 290 295 300 Gln Ile Phe Asn Lys Pro Tyr Trp Leu His Arg Ala Gln Gly His Asn 305 310 315 320 Asn Gly Ile Cys Trp Asn Asn Gln Leu Phe Ile Thr Cys Val Asp Thr 325 330 335 Thr Arg Ser Thr Asn Leu Thr Ile Ser Thr Ala Thr Ala Ala Val Ser 340 345 350 Pro Thr Phe Thr Pro Ser Asn Phe Lys Gln Tyr Ile Arg His Gly Glu 355 360 365 Glu Tyr Glu Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu Thr 370 375 380 Thr Glu Val Met Ala Tyr Leu His Thr Met Asp Pro Thr Ile Leu Glu 385 390 395 400 Gln Trp Asn Phe Gly Leu Thr Leu Pro Pro Ser Ala Ser Leu Glu Asp 405 410 415 Ala Tyr Arg Phe Val Arg Asn Ala Ala Thr Ser Cys Gln Lys Asp Thr 420 425 430 Pro Pro Gln Ala Lys Pro Asp Pro Leu Ala Lys Tyr Lys Phe Trp Asp 435 440 445 Val Asp Leu Lys Glu Arg Phe Ser Leu Asp Leu Asp Gln Phe Ala Leu 450 455 460 Gly Arg Lys Phe Leu Leu Gln Val Gly Val Lys Ala Lys Pro Lys Leu 465 470 475 480 Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr Ser Ser Ala Lys Arg Lys 485 490 495 Lys Val Lys Lys 500 <![CDATA[<210> 111]]> <![CDATA[<211> 1504]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5104 嵌合的HPV 51型L1蛋白的核苷酸序列]]> <![CDATA[<400> 111]]> atggctctgt ggaggaccaa tgacagcaag gtctacctgc ctcctgcccc tgtgagcagg 60 attgtgaaca cagaggaata catcaccagg acaggcatct actactatgc tggctccagc 120 agactgatta ccctgggaca cccatacttt ccaatcccaa agaccagcac cagggctgcc 180 atcccaaagg tgtctgcctt ccaatacagg gtgttcaggg tccaacttcc tgacccaaac 240 aagtttggac tgcctgaccc aaacctctac aaccctgaca cagacagact ggtgtggggc 300 tgtgtgggag tggaggtggg caggggacaa ccactgggag tgggactgtc tggacaccca 360 ctgttcaaca aatatgatga cacagagaac agcaggattg ccaatggcaa tgcccaacag 420 gatgtgaggg acaacacctc tgtggacaac aagcagaccc aactttgtat cattggctgt 480 gcccctccaa ttggagaaca ctggggcatt ggcaccactt gtaagaacac acctgtgcct 540 cctggagact gtcctccatt ggaactggtg tcctctgtga ttcaggatgg agatatgatt 600 gacacaggct ttggagctat ggactttgct gccctccaag ccaccaagtc tgatgtgcca 660 ctggacatca gccagtctgt gtgtaaatac cctgactacc tgaaaatgag tgctgacacc 720 tatggcaaca gtatgttctt ccacctgagg agggaacaga tttttgccag acactactac 780 aacaaactgg tgggagtggg agaggacatc ccaaatgact actacatcaa gggctctggc 840 aatggcaggg acccaattga gtcctacatc tactctgcca caccatctgg cagtatgatt 900 acctctgaca gccagatttt caacaagcca tactggctgc acagggctca aggacacaac 960 aatggcatct gttggaacaa ccaacttttc atcacttgtg tggacaccac caggagcacc 1020 aacctgacca tcagcacagc cacagcagca gtgagcccaa ccttcacacc aagcaacttc 1080 aagcaataca tcagacatgg agaggaatat gaactccaat tcatcttcca actttgtaag 1140 attaccctga ccacagaggt gatggcttac ctgcacacaa tggacccaac catcttggaa 1200 cagtggaact ttggactgac cctgcctcca tctgcctcct tggaggatgc ctacaggttt 1260 gtgaggaatg ctgccacctc ctgtcagaag gacacacctc cacaggctaa gcctgaccca 1320 ctggctaaat acaagttctg ggatgtggac ctgaaagaga ggttctccct ggacctggac 1380 cagtttgccc tgggcaggaa gttcctgctc caagtgggag tcaaagccaa gccaaaactg 1440 aaaagggctg ccccaaccag caccaggacc tcctctgcca agaggaagaa ggtgaagaag 1500 taaa 1504 <![CDATA[<210> 112]]> <![CDATA[<211> 1534]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<22]]>3> 5105 合成的HPV51L1基因]]> <br/> <br/><![CDATA[<400> 112]]> <br/><![CDATA[ctgggtacca tggctctgtg gaggaccaat gacagcaagg tctacctgcc tcctgcccct 60 gtgagcagga ttgtgaacac agaggaatac atcaccagga caggcatcta ctactatgct 120 ggctccagca gactgattac cctgggacac ccatactttc caatcccaaa gaccagcacc 180 agggctgcca tcccaaaggt gtctgccttc caatacaggg tgttcagggt ccaacttcct 240 gacccaaaca agtttggact gcctgaccca aacctctaca accctgacac agacagactg 300 gtgtggggct gtgtgggagt ggaggtgggc aggggacaac cactgggagt gggactgtct 360 ggacacccac tgttcaacaa atatgatgac acagagaaca gcaggattgc caatggcaat 420 gcccaacagg atgtgaggga caacacctct gtggacaaca agcagaccca actttgtatc 480 attggctgtg cccctccaat tggagaacac tggggcattg gcaccacttg taagaacaca 540 cctgtgcctc ctggagactg tcctccattg gaactggtgt cctctgtgat tcaggatgga 600 gatatgattg acacaggctt tggagctatg gactttgctg ccctccaagc caccaagtct 660 gatgtgccac tggacatcag ccagtctgtg tgtaaatacc ctgactacct gaaaatgagt 720 gctgacacct atggcaacag tatgttcttc cacctgagga gggaacagat ttttgccaga 780 cactactaca acaaactggt gggagtggga gaggacatcc caaatgacta ctacatcaag 840 ggctctggca atggcaggga cccaattgag tcctacatct actctgccac accatctggc 900 agtatgatta cctctgacag ccagattttc aacaagccat actggctgca cagggctcaa 960 ggacacaaca atggcatctg ttggaacaac caacttttca tcacttgtgt ggacaccacc 1020 aggagcacca acctgaccat cagcacagcc acagcagcag tgagcccaac cttcacacca 1080 agcaacttca agcaatacat cagacatgga gaggaatatg aactccaatt catcttccaa 1140 ctttgtaaga ttaccctgac cacagaggtg atggcttacc tgcacacaat ggacccaacc 1200 atcttggaac agtggaactt tggactgacc ctgcctccat ctgcctcctt ggaggatgcc 1260 tacaggtttg tgaggaatgc tgccacctcc tgtcagaagg acacacctcc acaggctaag 1320 cctgacccac tggctaaata caagttctgg gatgtggacc tgaaagagag gttctccctg 1380 gacctggacc agtttgccct gggcaggaag ttcctgctcc aagtgggagt ccagaggaag 1440 ccaagacctg gactgaaaag acctgcctcc tctgcctcct cctcctcctc ctcctctgcc 1500 aagaggaaga gggtgaagaa gtaaactcga gctc 1534 <![CDATA[<210> 113]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5106 合成的HPV33L1基因]]> <![CDATA[<400> 113]]> ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct 120 ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat 180 gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga 240 ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag 300 agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc 360 atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct 420 ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt 480 tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc 540 aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat 600 ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag 660 tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg 720 acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg 780 agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc 840 aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc 900 agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc 1020 aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag 1080 aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt 1140 tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc 1200 ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac 1260 aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag 1320 gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac 1380 ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca 1440 aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg 1500 aagaagtaaa ctcgagctc 1519 <![CDATA[<210> 114]]> <![CDATA[<211> 34]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5107 HPV51L1 F1]]> <![CDATA[<400> 114]]> cttggtacca tggctctgtg gaggaccaat gaca 34 <![CDATA[<210> 115]]> <![CDATA[<211> 32]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5108 HPV51L1 R1]]> <![CDATA[<400> 115]]> gcttggcttt gactcccact tggagcagga ac 32 <![CDATA[<210> 116]]> <![CDATA[<211> 1441]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5109 HPV51L1擴增序列1]]> <![CDATA[<400> 116]]> cttggtacca tggctctgtg gaggaccaat gacagcaagg tctacctgcc tcctgcccct 60 gtgagcagga ttgtgaacac agaggaatac atcaccagga caggcatcta ctactatgct 120 ggctccagca gactgattac cctgggacac ccatactttc caatcccaaa gaccagcacc 180 agggctgcca tcccaaaggt gtctgccttc caatacaggg tgttcagggt ccaacttcct 240 gacccaaaca agtttggact gcctgaccca aacctctaca accctgacac agacagactg 300 gtgtggggct gtgtgggagt ggaggtgggc aggggacaac cactgggagt gggactgtct 360 ggacacccac tgttcaacaa atatgatgac acagagaaca gcaggattgc caatggcaat 420 gcccaacagg atgtgaggga caacacctct gtggacaaca agcagaccca actttgtatc 480 attggctgtg cccctccaat tggagaacac tggggcattg gcaccacttg taagaacaca 540 cctgtgcctc ctggagactg tcctccattg gaactggtgt cctctgtgat tcaggatgga 600 gatatgattg acacaggctt tggagctatg gactttgctg ccctccaagc caccaagtct 660 gatgtgccac tggacatcag ccagtctgtg tgtaaatacc ctgactacct gaaaatgagt 720 gctgacacct atggcaacag tatgttcttc cacctgagga gggaacagat ttttgccaga 780 cactactaca acaaactggt gggagtggga gaggacatcc caaatgacta ctacatcaag 840 ggctctggca atggcaggga cccaattgag tcctacatct actctgccac accatctggc 900 agtatgatta cctctgacag ccagattttc aacaagccat actggctgca cagggctcaa 960 ggacacaaca atggcatctg ttggaacaac caacttttca tcacttgtgt ggacaccacc 1020 aggagcacca acctgaccat cagcacagcc acagcagcag tgagcccaac cttcacacca 1080 agcaacttca agcaatacat cagacatgga gaggaatatg aactccaatt catcttccaa 1140 ctttgtaaga ttaccctgac cacagaggtg atggcttacc tgcacacaat ggacccaacc 1200 atcttggaac agtggaactt tggactgacc ctgcctccat ctgcctcctt ggaggatgcc 1260 tacaggtttg tgaggaatgc tgccacctcc tgtcagaagg acacacctcc acaggctaag 1320 cctgacccac tggctaaata caagttctgg gatgtggacc tgaaagagag gttctccctg 1380 gacctggacc agtttgccct gggcaggaag ttcctgctcc aagtgggagt caaagccaag 1440 c 1441 <![CDATA[<210> 117]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5110 HPV51L1 F2]]> <![CDATA[<400> 117]]> agtgggagtc aaagccaagc caaaactgaa aaggg 35 <![CDATA[<210> 118]]> <![CDATA[<211> 36]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5111 HPV51L1 R2]]> <![CDATA[<400> 118]]> ctgtctagat ttacttcttc accttcttcc tcttgg 36 <![CDATA[<210> 119]]> <![CDATA[<211> 101]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5112 HPV51L1擴增序列2]]> <![CDATA[<400> 119]]> agtgggagtc aaagccaagc caaaactgaa aagggctgcc ccaaccagca ccaggacctc 60 ctctgccaag aggaagaagg tgaagaagta aatctagaca g 101 <![CDATA[<210> 120]]> <![CDATA[<211> 38]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5113 HPV 59型L1蛋白的471-508胺基酸序列]]> <![CDATA[<400> 120]]> Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 1 5 10 15 Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 20 25 30 Arg Lys Ser Ser Arg Lys 35 <![CDATA[<210> 121]]> <![CDATA[<211> 478]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5201 HPV 52型L1蛋白的1-478胺基酸序列]]> <![CDATA[<400> 121]]> Met Ser Val Trp Arg Pro Ser Glu Ala Thr Val Tyr Leu Pro Pro Val 1 5 10 15 Pro Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Ser Arg Thr Ser 20 25 30 Ile Tyr Tyr Tyr Ala Gly Ser Ser Arg Leu Leu Thr Val Gly His Pro 35 40 45 Tyr Phe Ser Ile Lys Asn Thr Ser Ser Gly Asn Gly Lys Lys Val Leu 50 55 60 Val Pro Lys Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Ile Lys Leu 65 70 75 80 Pro Asp Pro Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asn Pro 85 90 95 Glu Thr Gln Arg Leu Val Trp Ala Cys Thr Gly Leu Glu Ile Gly Arg 100 105 110 Gly Gln Pro Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys 115 120 125 Phe Asp Asp Thr Glu Thr Ser Asn Lys Tyr Ala Gly Lys Pro Gly Ile 130 135 140 Asp Asn Arg Glu Cys Leu Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys 145 150 155 160 Ile Leu Gly Cys Lys Pro Pro Ile Gly Glu His Trp Gly Lys Gly Thr 165 170 175 Pro Cys Asn Asn Asn Ser Gly Asn Pro Gly Asp Cys Pro Pro Leu Gln 180 185 190 Leu Ile Asn Ser Val Ile Gln Asp Gly Asp Met Val Asp Thr Gly Phe 195 200 205 Gly Cys Met Asp Phe Asn Thr Leu Gln Ala Ser Lys Ser Asp Val Pro 210 215 220 Ile Asp Ile Cys Ser Ser Val Cys Lys Tyr Pro Asp Tyr Leu Gln Met 225 230 235 240 Ala Ser Glu Pro Tyr Gly Asp Ser Leu Phe Phe Phe Leu Arg Arg Glu 245 250 255 Gln Met Phe Val Arg His Phe Phe Asn Arg Ala Gly Thr Leu Gly Asp 260 265 270 Pro Val Pro Gly Asp Leu Tyr Ile Gln Gly Ser Asn Ser Gly Asn Thr 275 280 285 Ala Thr Val Gln Ser Ser Ala Phe Phe Pro Thr Pro Ser Gly Ser Met 290 295 300 Val Thr Ser Glu Ser Gln Leu Phe Asn Lys Pro Tyr Trp Leu Gln Arg 305 310 315 320 Ala Gln Gly His Asn Asn Gly Ile Cys Trp Gly Asn Gln Leu Phe Val 325 330 335 Thr Val Val Asp Thr Thr Arg Ser Thr Asn Met Thr Leu Cys Ala Glu 340 345 350 Val Lys Lys Glu Ser Thr Tyr Lys Asn Glu Asn Phe Lys Glu Tyr Leu 355 360 365 Arg His Gly Glu Glu Phe Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys 370 375 380 Ile Thr Leu Thr Ala Asp Val Met Thr Tyr Ile His Lys Met Asp Ala 385 390 395 400 Thr Ile Leu Glu Asp Trp Gln Phe Gly Leu Thr Pro Pro Pro Ser Ala 405 410 415 Ser Leu Glu Asp Thr Tyr Arg Phe Val Thr Ser Thr Ala Ile Thr Cys 420 425 430 Gln Lys Asn Thr Pro Pro Lys Gly Lys Glu Asp Pro Leu Lys Asp Tyr 435 440 445 Met Phe Trp Glu Val Asp Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp 450 455 460 Gln Phe Pro Leu Gly Arg Lys Phe Leu Leu Gln Ala Gly Leu 465 470 475 <![CDATA[<210> 122]]> <![CDATA[<211> 26]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5202 HPV 33型L1蛋白的474-499胺基酸序列]]> <![CDATA[<400> 122]]> Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr 1 5 10 15 Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 20 25 <![CDATA[<210> 123]]> <![CDATA[<211> 504]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5203 嵌合的HPV 52型L1蛋白的胺基酸序列]]> <![CDATA[<400> 123]]> Met Ser Val Trp Arg Pro Ser Glu Ala Thr Val Tyr Leu Pro Pro Val 1 5 10 15 Pro Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Ser Arg Thr Ser 20 25 30 Ile Tyr Tyr Tyr Ala Gly Ser Ser Arg Leu Leu Thr Val Gly His Pro 35 40 45 Tyr Phe Ser Ile Lys Asn Thr Ser Ser Gly Asn Gly Lys Lys Val Leu 50 55 60 Val Pro Lys Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Ile Lys Leu 65 70 75 80 Pro Asp Pro Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asn Pro 85 90 95 Glu Thr Gln Arg Leu Val Trp Ala Cys Thr Gly Leu Glu Ile Gly Arg 100 105 110 Gly Gln Pro Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys 115 120 125 Phe Asp Asp Thr Glu Thr Ser Asn Lys Tyr Ala Gly Lys Pro Gly Ile 130 135 140 Asp Asn Arg Glu Cys Leu Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys 145 150 155 160 Ile Leu Gly Cys Lys Pro Pro Ile Gly Glu His Trp Gly Lys Gly Thr 165 170 175 Pro Cys Asn Asn Asn Ser Gly Asn Pro Gly Asp Cys Pro Pro Leu Gln 180 185 190 Leu Ile Asn Ser Val Ile Gln Asp Gly Asp Met Val Asp Thr Gly Phe 195 200 205 Gly Cys Met Asp Phe Asn Thr Leu Gln Ala Ser Lys Ser Asp Val Pro 210 215 220 Ile Asp Ile Cys Ser Ser Val Cys Lys Tyr Pro Asp Tyr Leu Gln Met 225 230 235 240 Ala Ser Glu Pro Tyr Gly Asp Ser Leu Phe Phe Phe Leu Arg Arg Glu 245 250 255 Gln Met Phe Val Arg His Phe Phe Asn Arg Ala Gly Thr Leu Gly Asp 260 265 270 Pro Val Pro Gly Asp Leu Tyr Ile Gln Gly Ser Asn Ser Gly Asn Thr 275 280 285 Ala Thr Val Gln Ser Ser Ala Phe Phe Pro Thr Pro Ser Gly Ser Met 290 295 300 Val Thr Ser Glu Ser Gln Leu Phe Asn Lys Pro Tyr Trp Leu Gln Arg 305 310 315 320 Ala Gln Gly His Asn Asn Gly Ile Cys Trp Gly Asn Gln Leu Phe Val 325 330 335 Thr Val Val Asp Thr Thr Arg Ser Thr Asn Met Thr Leu Cys Ala Glu 340 345 350 Val Lys Lys Glu Ser Thr Tyr Lys Asn Glu Asn Phe Lys Glu Tyr Leu 355 360 365 Arg His Gly Glu Glu Phe Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys 370 375 380 Ile Thr Leu Thr Ala Asp Val Met Thr Tyr Ile His Lys Met Asp Ala 385 390 395 400 Thr Ile Leu Glu Asp Trp Gln Phe Gly Leu Thr Pro Pro Pro Ser Ala 405 410 415 Ser Leu Glu Asp Thr Tyr Arg Phe Val Thr Ser Thr Ala Ile Thr Cys 420 425 430 Gln Lys Asn Thr Pro Pro Lys Gly Lys Glu Asp Pro Leu Lys Asp Tyr 435 440 445 Met Phe Trp Glu Val Asp Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp 450 455 460 Gln Phe Pro Leu Gly Arg Lys Phe Leu Leu Gln Ala Gly Leu Lys Ala 465 470 475 480 Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr Ser Ser 485 490 495 Ala Lys Arg Lys Lys Val Lys Lys 500 <![CDATA[<210> 124]]> <![CDATA[<211> 1516]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5204 嵌合的HPV 52型L1蛋白的核苷酸序列]]> <![CDATA[<400> 124]]> atgagcgtgt ggaggcccag cgaggccacc gtgtacctgc cccccgtgcc cgtgagcaag 60 gtggtgagca ccgacgagta cgtgagcagg accagcatct actactacgc cggcagcagc 120 aggctgctga ccgtgggcca cccctacttc agcatcaaga acaccagcag cggcaacggc 180 aagaaggtgc tggtgcccaa ggtgagcggc ctgcagtaca gggtgttcag gatcaagctg 240 cccgacccca acaagttcgg cttccccgac accagcttct acaaccccga gacccagagg 300 ctggtgtggg cctgcaccgg cctggagatc ggcaggggcc agcccctggg cgtgggcatc 360 agcggccacc ccctgctgaa caagttcgac gacaccgaga ccagcaacaa gtacgccggc 420 aagcccggca tcgacaacag ggagtgcctg agcatggact acaagcagac ccagctgtgc 480 atcctgggct gcaagccccc catcggcgag cactggggca agggcacccc ctgcaacaac 540 aacagcggca accccggcga ctgccccccc ctgcagctga tcaacagcgt gatccaggac 600 ggcgacatgg tggacaccgg cttcggctgc atggacttca acaccctgca ggccagcaag 660 agcgacgtgc ccatcgacat ctgcagcagc gtgtgcaagt accccgacta cctgcagatg 720 gccagcgagc cctacggcga cagcctgttc ttcttcctga ggagggagca gatgttcgtg 780 aggcacttct tcaacagggc cggcaccctg ggcgaccccg tgcccggcga cctgtacatc 840 cagggcagca acagcggcaa caccgccacc gtgcagagca gcgccttctt ccccaccccc 900 agcggcagca tggtgaccag cgagagccag ctgttcaaca agccctactg gctgcagagg 960 gcccagggcc acaacaacgg catctgctgg ggcaaccagc tgttcgtgac cgtggtggac 1020 accaccagga gcaccaacat gaccctgtgc gccgaggtga agaaggagag cacctacaag 1080 aacgagaact tcaaggagta cctgaggcac ggcgaggagt tcgacctgca gttcatcttc 1140 cagctgtgca agatcaccct gaccgccgac gtgatgacct acatccacaa gatggacgcc 1200 accatcctgg aggactggca gttcggcctg accccccccc ccagcgccag cctggaggac 1260 acctacaggt tcgtgaccag caccgccatc acctgccaga agaacacccc ccccaagggc 1320 aaggaggacc ccctgaagga ctacatgttc tgggaggtgg acctgaagga gaagttcagc 1380 gccgacctgg accagttccc cctgggcagg aagttcctgc tgcaggccgg cctgaaagcc 1440 aagccaaaac tgaaaagggc tgccccaacc agcaccagga cctcctctgc caagaggaag 1500 aaggtgaaga agtaaa 1516 <![CDATA[<210> 125]]> <![CDATA[<211> 1531]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5205 合成的HPV52L1基因]]> <![CDATA[<400> 125]]> ctgggtacca tgagcgtgtg gaggcccagc gaggccaccg tgtacctgcc ccccgtgccc 60 gtgagcaagg tggtgagcac cgacgagtac gtgagcagga ccagcatcta ctactacgcc 120 ggcagcagca ggctgctgac cgtgggccac ccctacttca gcatcaagaa caccagcagc 180 ggcaacggca agaaggtgct ggtgcccaag gtgagcggcc tgcagtacag ggtgttcagg 240 atcaagctgc ccgaccccaa caagttcggc ttccccgaca ccagcttcta caaccccgag 300 acccagaggc tggtgtgggc ctgcaccggc ctggagatcg gcaggggcca gcccctgggc 360 gtgggcatca gcggccaccc cctgctgaac aagttcgacg acaccgagac cagcaacaag 420 tacgccggca agcccggcat cgacaacagg gagtgcctga gcatggacta caagcagacc 480 cagctgtgca tcctgggctg caagcccccc atcggcgagc actggggcaa gggcaccccc 540 tgcaacaaca acagcggcaa ccccggcgac tgcccccccc tgcagctgat caacagcgtg 600 atccaggacg gcgacatggt ggacaccggc ttcggctgca tggacttcaa caccctgcag 660 gccagcaaga gcgacgtgcc catcgacatc tgcagcagcg tgtgcaagta ccccgactac 720 ctgcagatgg ccagcgagcc ctacggcgac agcctgttct tcttcctgag gagggagcag 780 atgttcgtga ggcacttctt caacagggcc ggcaccctgg gcgaccccgt gcccggcgac 840 ctgtacatcc agggcagcaa cagcggcaac accgccaccg tgcagagcag cgccttcttc 900 cccaccccca gcggcagcat ggtgaccagc gagagccagc tgttcaacaa gccctactgg 960 ctgcagaggg cccagggcca caacaacggc atctgctggg gcaaccagct gttcgtgacc 1020 gtggtggaca ccaccaggag caccaacatg accctgtgcg ccgaggtgaa gaaggagagc 1080 acctacaaga acgagaactt caaggagtac ctgaggcacg gcgaggagtt cgacctgcag 1140 ttcatcttcc agctgtgcaa gatcaccctg accgccgacg tgatgaccta catccacaag 1200 atggacgcca ccatcctgga ggactggcag ttcggcctga cccccccccc cagcgccagc 1260 ctggaggaca cctacaggtt cgtgaccagc accgccatca cctgccagaa gaacaccccc 1320 cccaagggca aggaggaccc cctgaaggac tacatgttct gggaggtgga cctgaaggag 1380 aagttcagcg ccgacctgga ccagttcccc ctgggcagga agttcctgct gcaggccggc 1440 ctgcaggcca ggcccaagct gaagaggccc gccagcagcg cccccaggac cagcaccaag 1500 aagaagaagg tgaagaggta aactcgagct c 1531 <![CDATA[<210> 126]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<22]]>0> ]]> <br/><![CDATA[<223> 5206 合成的HPV33L1基因]]> <br/> <br/><![CDATA[<400> 126]]> <br/><![CDATA[ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct 120 ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat 180 gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga 240 ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag 300 agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc 360 atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct 420 ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt 480 tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc 540 aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat 600 ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag 660 tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg 720 acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg 780 agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc 840 aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc 900 agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc 1020 aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag 1080 aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt 1140 tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc 1200 ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac 1260 aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag 1320 gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac 1380 ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca 1440 aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg 1500 aagaagtaaa ctcgagctc 1519 <![CDATA[<210> 127]]> <![CDATA[<211> 34]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5207 HPV52L1 F1]]> <![CDATA[<400> 127]]> cttggtacca tgagcgtgtg gaggcccagc gagg 34 <![CDATA[<210> 128]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5208 HPV52L1 R1]]> <![CDATA[<400> 128]]> gcttggcttt caggccggcc tgcagcagga acttc 35 <![CDATA[<210> 129]]> <![CDATA[<211> 1453]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5209 HPV52L1擴增序列1]]> <![CDATA[<400> 129]]> cttggtacca tgagcgtgtg gaggcccagc gaggccaccg tgtacctgcc ccccgtgccc 60 gtgagcaagg tggtgagcac cgacgagtac gtgagcagga ccagcatcta ctactacgcc 120 ggcagcagca ggctgctgac cgtgggccac ccctacttca gcatcaagaa caccagcagc 180 ggcaacggca agaaggtgct ggtgcccaag gtgagcggcc tgcagtacag ggtgttcagg 240 atcaagctgc ccgaccccaa caagttcggc ttccccgaca ccagcttcta caaccccgag 300 acccagaggc tggtgtgggc ctgcaccggc ctggagatcg gcaggggcca gcccctgggc 360 gtgggcatca gcggccaccc cctgctgaac aagttcgacg acaccgagac cagcaacaag 420 tacgccggca agcccggcat cgacaacagg gagtgcctga gcatggacta caagcagacc 480 cagctgtgca tcctgggctg caagcccccc atcggcgagc actggggcaa gggcaccccc 540 tgcaacaaca acagcggcaa ccccggcgac tgcccccccc tgcagctgat caacagcgtg 600 atccaggacg gcgacatggt ggacaccggc ttcggctgca tggacttcaa caccctgcag 660 gccagcaaga gcgacgtgcc catcgacatc tgcagcagcg tgtgcaagta ccccgactac 720 ctgcagatgg ccagcgagcc ctacggcgac agcctgttct tcttcctgag gagggagcag 780 atgttcgtga ggcacttctt caacagggcc ggcaccctgg gcgaccccgt gcccggcgac 840 ctgtacatcc agggcagcaa cagcggcaac accgccaccg tgcagagcag cgccttcttc 900 cccaccccca gcggcagcat ggtgaccagc gagagccagc tgttcaacaa gccctactgg 960 ctgcagaggg cccagggcca caacaacggc atctgctggg gcaaccagct gttcgtgacc 1020 gtggtggaca ccaccaggag caccaacatg accctgtgcg ccgaggtgaa gaaggagagc 1080 acctacaaga acgagaactt caaggagtac ctgaggcacg gcgaggagtt cgacctgcag 1140 ttcatcttcc agctgtgcaa gatcaccctg accgccgacg tgatgaccta catccacaag 1200 atggacgcca ccatcctgga ggactggcag ttcggcctga cccccccccc cagcgccagc 1260 ctggaggaca cctacaggtt cgtgaccagc accgccatca cctgccagaa gaacaccccc 1320 cccaagggca aggaggaccc cctgaaggac tacatgttct gggaggtgga cctgaaggag 1380 aagttcagcg ccgacctgga ccagttcccc ctgggcagga agttcctgct gcaggccggc 1440 ctgaaagcca agc 1453 <![CDATA[<210> 130]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5210 HPV52L1 F2]]> <![CDATA[<400> 130]]> ggccggcctg aaagccaagc caaaactgaa aaggg 35 <![CDATA[<210> 131]]> <![CDATA[<211> 36]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5211 HPV52L1 R2]]> <![CDATA[<400> 131]]> ctgtctagat ttacttcttc accttcttcc tcttgg 36 <![CDATA[<210> 132]]> <![CDATA[<211> 101]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5212 HPV52L1擴增序列2]]> <![CDATA[<400> 132]]> ggccggcctg aaagccaagc caaaactgaa aagggctgcc ccaaccagca ccaggacctc 60 ctctgccaag aggaagaagg tgaagaagta aatctagaca g 101 <![CDATA[<210> 133]]> <![CDATA[<211> 38]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5213 HPV 59型L1蛋白的471-508胺基酸序列]]> <![CDATA[<400> 133]]> Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 1 5 10 15 Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 20 25 30 Arg Lys Ser Ser Arg Lys 35 <![CDATA[<210> 134]]> <![CDATA[<211> 467]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5601 HPV 56型L1蛋白的1-467胺基酸序列]]> <![CDATA[<400> 134]]> Met Ala Thr Trp Arg Pro Ser Glu Asn Lys Val Tyr Leu Pro Pro Thr 1 5 10 15 Pro Val Ser Lys Val Val Ala Thr Asp Ser Tyr Val Lys Arg Thr Ser 20 25 30 Ile Phe Tyr His Ala Gly Ser Ser Arg Leu Leu Ala Val Gly His Pro 35 40 45 Tyr Tyr Ser Val Thr Lys Asp Asn Thr Lys Thr Asn Ile Pro Lys Val 50 55 60 Ser Ala Tyr Gln Tyr Arg Val Phe Arg Val Arg Leu Pro Asp Pro Asn 65 70 75 80 Lys Phe Gly Leu Pro Asp Thr Asn Ile Tyr Asn Pro Asp Gln Glu Arg 85 90 95 Leu Val Trp Ala Cys Val Gly Leu Glu Val Gly Arg Gly Gln Pro Leu 100 105 110 Gly Ala Gly Leu Ser Gly His Pro Leu Phe Asn Arg Leu Asp Asp Thr 115 120 125 Glu Ser Ser Asn Leu Ala Asn Asn Asn Val Ile Glu Asp Ser Arg Asp 130 135 140 Asn Ile Ser Val Asp Gly Lys Gln Thr Gln Leu Cys Ile Val Gly Cys 145 150 155 160 Thr Pro Ala Met Gly Glu His Trp Thr Lys Gly Ala Val Cys Lys Ser 165 170 175 Thr Gln Val Thr Thr Gly Asp Cys Pro Pro Leu Ala Leu Ile Asn Thr 180 185 190 Pro Ile Glu Asp Gly Asp Met Ile Asp Thr Gly Phe Gly Ala Met Asp 195 200 205 Phe Lys Val Leu Gln Glu Ser Lys Ala Glu Val Pro Leu Asp Ile Val 210 215 220 Gln Ser Thr Cys Lys Tyr Pro Asp Tyr Leu Lys Met Ser Ala Asp Ala 225 230 235 240 Tyr Gly Asp Ser Met Trp Phe Tyr Leu Arg Arg Glu Gln Leu Phe Ala 245 250 255 Arg His Tyr Phe Asn Arg Ala Gly Lys Val Gly Glu Thr Ile Pro Ala 260 265 270 Glu Leu Tyr Leu Lys Gly Ser Asn Gly Arg Glu Pro Pro Pro Ser Ser 275 280 285 Val Tyr Val Ala Thr Pro Ser Gly Ser Met Ile Thr Ser Glu Ala Gln 290 295 300 Leu Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn Asn 305 310 315 320 Gly Ile Cys Trp Gly Asn Gln Leu Phe Val Thr Val Val Asp Thr Thr 325 330 335 Arg Ser Thr Asn Met Thr Ile Ser Thr Ala Thr Glu Gln Leu Ser Lys 340 345 350 Tyr Asp Ala Arg Lys Ile Asn Gln Tyr Leu Arg His Val Glu Glu Tyr 355 360 365 Glu Leu Gln Phe Val Phe Gln Leu Cys Lys Ile Thr Leu Ser Ala Glu 370 375 380 Val Met Ala Tyr Leu His Asn Met Asn Ala Asn Leu Leu Glu Asp Trp 385 390 395 400 Asn Ile Gly Leu Ser Pro Pro Val Ala Thr Ser Leu Glu Asp Lys Tyr 405 410 415 Arg Tyr Val Arg Ser Thr Ala Ile Thr Cys Gln Arg Glu Gln Pro Pro 420 425 430 Thr Glu Lys Gln Asp Pro Leu Ala Lys Tyr Lys Phe Trp Asp Val Asn 435 440 445 Leu Gln Asp Ser Phe Ser Thr Asp Leu Asp Gln Phe Pro Leu Gly Arg 450 455 460 Lys Phe Leu 465 <![CDATA[<210> 135]]> <![CDATA[<211> 31]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5602 HPV 33型L1蛋白的469-499胺基酸序列]]> <![CDATA[<400> 135]]> Leu Gln Ala Gly Leu Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro 1 5 10 15 Thr Ser Thr Arg Thr Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 20 25 30 <![CDATA[<210> 136]]> <![CDATA[<211> 498]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5603 嵌合的HPV 56型L1蛋白的胺基酸序列]]> <![CDATA[<400> 136]]> Met Ala Thr Trp Arg Pro Ser Glu Asn Lys Val Tyr Leu Pro Pro Thr 1 5 10 15 Pro Val Ser Lys Val Val Ala Thr Asp Ser Tyr Val Lys Arg Thr Ser 20 25 30 Ile Phe Tyr His Ala Gly Ser Ser Arg Leu Leu Ala Val Gly His Pro 35 40 45 Tyr Tyr Ser Val Thr Lys Asp Asn Thr Lys Thr Asn Ile Pro Lys Val 50 55 60 Ser Ala Tyr Gln Tyr Arg Val Phe Arg Val Arg Leu Pro Asp Pro Asn 65 70 75 80 Lys Phe Gly Leu Pro Asp Thr Asn Ile Tyr Asn Pro Asp Gln Glu Arg 85 90 95 Leu Val Trp Ala Cys Val Gly Leu Glu Val Gly Arg Gly Gln Pro Leu 100 105 110 Gly Ala Gly Leu Ser Gly His Pro Leu Phe Asn Arg Leu Asp Asp Thr 115 120 125 Glu Ser Ser Asn Leu Ala Asn Asn Asn Val Ile Glu Asp Ser Arg Asp 130 135 140 Asn Ile Ser Val Asp Gly Lys Gln Thr Gln Leu Cys Ile Val Gly Cys 145 150 155 160 Thr Pro Ala Met Gly Glu His Trp Thr Lys Gly Ala Val Cys Lys Ser 165 170 175 Thr Gln Val Thr Thr Gly Asp Cys Pro Pro Leu Ala Leu Ile Asn Thr 180 185 190 Pro Ile Glu Asp Gly Asp Met Ile Asp Thr Gly Phe Gly Ala Met Asp 195 200 205 Phe Lys Val Leu Gln Glu Ser Lys Ala Glu Val Pro Leu Asp Ile Val 210 215 220 Gln Ser Thr Cys Lys Tyr Pro Asp Tyr Leu Lys Met Ser Ala Asp Ala 225 230 235 240 Tyr Gly Asp Ser Met Trp Phe Tyr Leu Arg Arg Glu Gln Leu Phe Ala 245 250 255 Arg His Tyr Phe Asn Arg Ala Gly Lys Val Gly Glu Thr Ile Pro Ala 260 265 270 Glu Leu Tyr Leu Lys Gly Ser Asn Gly Arg Glu Pro Pro Pro Ser Ser 275 280 285 Val Tyr Val Ala Thr Pro Ser Gly Ser Met Ile Thr Ser Glu Ala Gln 290 295 300 Leu Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn Asn 305 310 315 320 Gly Ile Cys Trp Gly Asn Gln Leu Phe Val Thr Val Val Asp Thr Thr 325 330 335 Arg Ser Thr Asn Met Thr Ile Ser Thr Ala Thr Glu Gln Leu Ser Lys 340 345 350 Tyr Asp Ala Arg Lys Ile Asn Gln Tyr Leu Arg His Val Glu Glu Tyr 355 360 365 Glu Leu Gln Phe Val Phe Gln Leu Cys Lys Ile Thr Leu Ser Ala Glu 370 375 380 Val Met Ala Tyr Leu His Asn Met Asn Ala Asn Leu Leu Glu Asp Trp 385 390 395 400 Asn Ile Gly Leu Ser Pro Pro Val Ala Thr Ser Leu Glu Asp Lys Tyr 405 410 415 Arg Tyr Val Arg Ser Thr Ala Ile Thr Cys Gln Arg Glu Gln Pro Pro 420 425 430 Thr Glu Lys Gln Asp Pro Leu Ala Lys Tyr Lys Phe Trp Asp Val Asn 435 440 445 Leu Gln Asp Ser Phe Ser Thr Asp Leu Asp Gln Phe Pro Leu Gly Arg 450 455 460 Lys Phe Leu Leu Gln Ala Gly Leu Lys Ala Lys Pro Lys Leu Lys Arg 465 470 475 480 Ala Ala Pro Thr Ser Thr Arg Thr Ser Ser Ala Lys Arg Lys Lys Val 485 490 495 Lys Lys <![CDATA[<210> 137]]> <![CDATA[<211> 1498]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5604 嵌合的HPV 56型L1蛋白的核苷酸序列]]> <![CDATA[<400> 137]]> atggctacct ggagaccatc tgagaacaag gtctacctgc ctccaacacc tgtgagcaag 60 gtggtggcta cagactccta tgtgaagagg accagcatct tctaccatgc tggctccagc 120 agactgctgg ctgtgggaca cccatactac tctgtgacca aggacaacac caagaccaac 180 atcccaaagg tgtctgccta ccaatacagg gtgttcaggg tgagactgcc tgacccaaac 240 aagtttggac tgcctgacac caacatctac aaccctgacc aggagagact ggtgtgggct 300 tgtgtgggat tggaggtggg caggggacaa ccactgggag caggactgtc tggacaccca 360 ctgttcaaca gactggatga cacagagtcc agcaacctgg ctaacaacaa tgtgattgag 420 gacagcaggg acaacatctc tgtggatggc aagcagaccc aactttgtat tgtgggctgt 480 actcctgcta tgggagaaca ctggaccaag ggagcagtgt gtaagagcac ccaggtgacc 540 acaggagact gtcctccact ggctctgata aacacaccaa ttgaggatgg agatatgatt 600 gacacaggct ttggagctat ggacttcaag gtgctccaag agagcaaggc tgaggtgcca 660 ctggacattg tccagagcac ttgtaaatac cctgactacc tgaaaatgag tgctgatgcc 720 tatggagaca gtatgtggtt ctacctgagg agggaacaac tttttgccag acactacttc 780 aacagggctg gcaaggtggg agagaccatc cctgctgaac tctacctgaa aggcagcaat 840 ggcagggaac ctcctccatc ctctgtctat gtggctacac catctggcag tatgattacc 900 tctgaggctc aacttttcaa caagccatac tggctccaaa gggctcaagg acacaacaat 960 ggcatctgtt ggggcaacca actttttgtg acagtggtgg acaccaccag gagcaccaat 1020 atgaccatca gcacagccac agaacaactt agcaaatatg atgccaggaa gataaaccaa 1080 tacctgaggc atgtggagga atatgaactc caatttgtgt tccaactttg taagattacc 1140 ctgtctgctg aggtgatggc ttacctgcac aatatgaatg ccaacctgtt ggaggactgg 1200 aacattggac tgagccctcc tgtggctacc tccttggagg acaaatacag atatgtgagg 1260 agcacagcca tcacttgtca gagggaacaa cctccaacag agaagcagga cccactggct 1320 aaatacaagt tctgggatgt gaacctccaa gactccttca gcacagacct ggaccagttt 1380 ccactgggca ggaagttcct gctccaagca ggactgaaag ccaagccaaa actgaaaagg 1440 gctgccccaa ccagcaccag gacctcctct gccaagagga agaaggtgaa gaagtaaa 1498 <![CDATA[<210> 138]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5605 合成的HPV56L1基因]]> <![CDATA[<400> 138]]> ctgggtacca tggctacctg gagaccatct gagaacaagg tctacctgcc tccaacacct 60 gtgagcaagg tggtggctac agactcctat gtgaagagga ccagcatctt ctaccatgct 120 ggctccagca gactgctggc tgtgggacac ccatactact ctgtgaccaa ggacaacacc 180 aagaccaaca tcccaaaggt gtctgcctac caatacaggg tgttcagggt gagactgcct 240 gacccaaaca agtttggact gcctgacacc aacatctaca accctgacca ggagagactg 300 gtgtgggctt gtgtgggatt ggaggtgggc aggggacaac cactgggagc aggactgtct 360 ggacacccac tgttcaacag actggatgac acagagtcca gcaacctggc taacaacaat 420 gtgattgagg acagcaggga caacatctct gtggatggca agcagaccca actttgtatt 480 gtgggctgta ctcctgctat gggagaacac tggaccaagg gagcagtgtg taagagcacc 540 caggtgacca caggagactg tcctccactg gctctgataa acacaccaat tgaggatgga 600 gatatgattg acacaggctt tggagctatg gacttcaagg tgctccaaga gagcaaggct 660 gaggtgccac tggacattgt ccagagcact tgtaaatacc ctgactacct gaaaatgagt 720 gctgatgcct atggagacag tatgtggttc tacctgagga gggaacaact ttttgccaga 780 cactacttca acagggctgg caaggtggga gagaccatcc ctgctgaact ctacctgaaa 840 ggcagcaatg gcagggaacc tcctccatcc tctgtctatg tggctacacc atctggcagt 900 atgattacct ctgaggctca acttttcaac aagccatact ggctccaaag ggctcaagga 960 cacaacaatg gcatctgttg gggcaaccaa ctttttgtga cagtggtgga caccaccagg 1020 agcaccaata tgaccatcag cacagccaca gaacaactta gcaaatatga tgccaggaag 1080 ataaaccaat acctgaggca tgtggaggaa tatgaactcc aatttgtgtt ccaactttgt 1140 aagattaccc tgtctgctga ggtgatggct tacctgcaca atatgaatgc caacctgttg 1200 gaggactgga acattggact gagccctcct gtggctacct ccttggagga caaatacaga 1260 tatgtgagga gcacagccat cacttgtcag agggaacaac ctccaacaga gaagcaggac 1320 ccactggcta aatacaagtt ctgggatgtg aacctccaag actccttcag cacagacctg 1380 gaccagtttc cactgggcag gaagttcctg atgcaacttg gcaccaggag caagcctgct 1440 gtggctacca gcaagaagag gtctgcccca accagcacca gcacacctgc caagaggaag 1500 aggaggtaaa ctcgagctc 1519 <![CDATA[<210> 139]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5606 合成的HPV33L1基因]]> <![CDATA[<400> 139]]> ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct 120 ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat 180 gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga 240 ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag 300 agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc 360 atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct 420 ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt 480 tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc 540 aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat 600 ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag 660 tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg 720 acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg 780 agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc 840 aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc 900 agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc 1020 aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag 1080 aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt 1140 tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc 1200 ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac 1260 aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag 1320 gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac 1380 ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca 1440 aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg 1500 aagaagtaaa ctcgagctc 1519 <![CDATA[<210> 140]]> <![CDATA[<211> 33]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5607 HPV56L1 F1]]> <![CDATA[<400> 140]]> cttggtacca tggctacctg gagaccatct gag 33 <![CDATA[<210> 141]]> <![CDATA[<211> 31]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5608 HPV56L1 R1]]> <![CDATA[<400> 141]]> ctgcttggag caggaacttc ctgcccagtg g 31 <![CDATA[<210> 142]]> <![CDATA[<211> 1420]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5609 HPV56L1擴增序列1]]> <![CDATA[<400> 142]]> cttggtacca tggctacctg gagaccatct gagaacaagg tctacctgcc tccaacacct 60 gtgagcaagg tggtggctac agactcctat gtgaagagga ccagcatctt ctaccatgct 120 ggctccagca gactgctggc tgtgggacac ccatactact ctgtgaccaa ggacaacacc 180 aagaccaaca tcccaaaggt gtctgcctac caatacaggg tgttcagggt gagactgcct 240 gacccaaaca agtttggact gcctgacacc aacatctaca accctgacca ggagagactg 300 gtgtgggctt gtgtgggatt ggaggtgggc aggggacaac cactgggagc aggactgtct 360 ggacacccac tgttcaacag actggatgac acagagtcca gcaacctggc taacaacaat 420 gtgattgagg acagcaggga caacatctct gtggatggca agcagaccca actttgtatt 480 gtgggctgta ctcctgctat gggagaacac tggaccaagg gagcagtgtg taagagcacc 540 caggtgacca caggagactg tcctccactg gctctgataa acacaccaat tgaggatgga 600 gatatgattg acacaggctt tggagctatg gacttcaagg tgctccaaga gagcaaggct 660 gaggtgccac tggacattgt ccagagcact tgtaaatacc ctgactacct gaaaatgagt 720 gctgatgcct atggagacag tatgtggttc tacctgagga gggaacaact ttttgccaga 780 cactacttca acagggctgg caaggtggga gagaccatcc ctgctgaact ctacctgaaa 840 ggcagcaatg gcagggaacc tcctccatcc tctgtctatg tggctacacc atctggcagt 900 atgattacct ctgaggctca acttttcaac aagccatact ggctccaaag ggctcaagga 960 cacaacaatg gcatctgttg gggcaaccaa ctttttgtga cagtggtgga caccaccagg 1020 agcaccaata tgaccatcag cacagccaca gaacaactta gcaaatatga tgccaggaag 1080 ataaaccaat acctgaggca tgtggaggaa tatgaactcc aatttgtgtt ccaactttgt 1140 aagattaccc tgtctgctga ggtgatggct tacctgcaca atatgaatgc caacctgttg 1200 gaggactgga acattggact gagccctcct gtggctacct ccttggagga caaatacaga 1260 tatgtgagga gcacagccat cacttgtcag agggaacaac ctccaacaga gaagcaggac 1320 ccactggcta aatacaagtt ctgggatgtg aacctccaag actccttcag cacagacctg 1380 gaccagtttc cactgggcag gaagttcctg ctccaagcag 1420 <![CDATA[<210> 143]]> <![CDATA[<211> 36]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5610 HPV56L1 F2]]> <![CDATA[<400> 143]]> gaagttcctg ctccaagcag gactgaaagc caagcc 36 <![CDATA[<210> 144]]> <![CDATA[<211> 36]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5611 HPV56L1 R2]]> <![CDATA[<400> 144]]> ctgtctagat ttacttcttc accttcttcc tcttgg 36 <![CDATA[<210> 145]]> <![CDATA[<211> 116]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5612 HPV56L1擴增序列2]]> <![CDATA[<400> 145]]> gaagttcctg ctccaagcag gactgaaagc caagccaaaa ctgaaaaggg ctgccccaac 60 cagcaccagg acctcctctg ccaagaggaa gaaggtgaag aagtaaatct agacag 116 <![CDATA[<210> 146]]> <![CDATA[<211> 38]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5613 HPV 59型L1蛋白的471-508胺基酸序列]]> <![CDATA[<400> 146]]> Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 1 5 10 15 Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 20 25 30 Arg Lys Ser Ser Arg Lys 35 <![CDATA[<210> 147]]> <![CDATA[<211> 473]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5801 HPV 58型L1蛋白的1-473胺基酸序列]]> <![CDATA[<400> 147]]> Met Ser Val Trp Arg Pro Ser Glu Ala Thr Val Tyr Leu Pro Pro Val 1 5 10 15 Pro Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Ser Arg Thr Ser 20 25 30 Ile Tyr Tyr Tyr Ala Gly Ser Ser Arg Leu Leu Ala Val Gly Asn Pro 35 40 45 Tyr Phe Ser Ile Lys Ser Pro Asn Asn Asn Lys Lys Val Leu Val Pro 50 55 60 Lys Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Val Arg Leu Pro Asp 65 70 75 80 Pro Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asn Pro Asp Thr 85 90 95 Gln Arg Leu Val Trp Ala Cys Val Gly Leu Glu Ile Gly Arg Gly Gln 100 105 110 Pro Leu Gly Val Gly Val Ser Gly His Pro Tyr Leu Asn Lys Phe Asp 115 120 125 Asp Thr Glu Thr Ser Asn Arg Tyr Pro Ala Gln Pro Gly Ser Asp Asn 130 135 140 Arg Glu Cys Leu Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Ile 145 150 155 160 Gly Cys Lys Pro Pro Thr Gly Glu His Trp Gly Lys Gly Val Ala Cys 165 170 175 Asn Asn Asn Ala Ala Ala Thr Asp Cys Pro Pro Leu Glu Leu Phe Asn 180 185 190 Ser Ile Ile Glu Asp Gly Asp Met Val Asp Thr Gly Phe Gly Cys Met 195 200 205 Asp Phe Gly Thr Leu Gln Ala Asn Lys Ser Asp Val Pro Ile Asp Ile 210 215 220 Cys Asn Ser Thr Cys Lys Tyr Pro Asp Tyr Leu Lys Met Ala Ser Glu 225 230 235 240 Pro Tyr Gly Asp Ser Leu Phe Phe Phe Leu Arg Arg Glu Gln Met Phe 245 250 255 Val Arg His Phe Phe Asn Arg Ala Gly Lys Leu Gly Glu Ala Val Pro 260 265 270 Asp Asp Leu Tyr Ile Lys Gly Ser Gly Asn Thr Ala Val Ile Gln Ser 275 280 285 Ser Ala Phe Phe Pro Thr Pro Ser Gly Ser Ile Val Thr Ser Glu Ser 290 295 300 Gln Leu Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn 305 310 315 320 Asn Gly Ile Cys Trp Gly Asn Gln Leu Phe Val Thr Val Val Asp Thr 325 330 335 Thr Arg Ser Thr Asn Met Thr Leu Cys Thr Glu Val Thr Lys Glu Gly 340 345 350 Thr Tyr Lys Asn Asp Asn Phe Lys Glu Tyr Val Arg His Val Glu Glu 355 360 365 Tyr Asp Leu Gln Phe Val Phe Gln Leu Cys Lys Ile Thr Leu Thr Ala 370 375 380 Glu Ile Met Thr Tyr Ile His Thr Met Asp Ser Asn Ile Leu Glu Asp 385 390 395 400 Trp Gln Phe Gly Leu Thr Pro Pro Pro Ser Ala Ser Leu Gln Asp Thr 405 410 415 Tyr Arg Phe Val Thr Ser Gln Ala Ile Thr Cys Gln Lys Thr Ala Pro 420 425 430 Pro Lys Glu Lys Glu Asp Pro Leu Asn Lys Tyr Thr Phe Trp Glu Val 435 440 445 Asn Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro Leu Gly 450 455 460 Arg Lys Phe Leu Leu Gln Ser Gly Leu 465 470 <![CDATA[<210> 148]]> <![CDATA[<211> 26]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5802 HPV 33型L1蛋白的474-499胺基酸序列]]> <![CDATA[<400> 148]]> Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr 1 5 10 15 Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 20 25 <![CDATA[<210> 149]]> <![CDATA[<211> 499]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5803 嵌合的HPV 58型L1蛋白的胺基酸序列]]> <![CDATA[<400> 149]]> Met Ser Val Trp Arg Pro Ser Glu Ala Thr Val Tyr Leu Pro Pro Val 1 5 10 15 Pro Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Ser Arg Thr Ser 20 25 30 Ile Tyr Tyr Tyr Ala Gly Ser Ser Arg Leu Leu Ala Val Gly Asn Pro 35 40 45 Tyr Phe Ser Ile Lys Ser Pro Asn Asn Asn Lys Lys Val Leu Val Pro 50 55 60 Lys Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Val Arg Leu Pro Asp 65 70 75 80 Pro Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asn Pro Asp Thr 85 90 95 Gln Arg Leu Val Trp Ala Cys Val Gly Leu Glu Ile Gly Arg Gly Gln 100 105 110 Pro Leu Gly Val Gly Val Ser Gly His Pro Tyr Leu Asn Lys Phe Asp 115 120 125 Asp Thr Glu Thr Ser Asn Arg Tyr Pro Ala Gln Pro Gly Ser Asp Asn 130 135 140 Arg Glu Cys Leu Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Ile 145 150 155 160 Gly Cys Lys Pro Pro Thr Gly Glu His Trp Gly Lys Gly Val Ala Cys 165 170 175 Asn Asn Asn Ala Ala Ala Thr Asp Cys Pro Pro Leu Glu Leu Phe Asn 180 185 190 Ser Ile Ile Glu Asp Gly Asp Met Val Asp Thr Gly Phe Gly Cys Met 195 200 205 Asp Phe Gly Thr Leu Gln Ala Asn Lys Ser Asp Val Pro Ile Asp Ile 210 215 220 Cys Asn Ser Thr Cys Lys Tyr Pro Asp Tyr Leu Lys Met Ala Ser Glu 225 230 235 240 Pro Tyr Gly Asp Ser Leu Phe Phe Phe Leu Arg Arg Glu Gln Met Phe 245 250 255 Val Arg His Phe Phe Asn Arg Ala Gly Lys Leu Gly Glu Ala Val Pro 260 265 270 Asp Asp Leu Tyr Ile Lys Gly Ser Gly Asn Thr Ala Val Ile Gln Ser 275 280 285 Ser Ala Phe Phe Pro Thr Pro Ser Gly Ser Ile Val Thr Ser Glu Ser 290 295 300 Gln Leu Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn 305 310 315 320 Asn Gly Ile Cys Trp Gly Asn Gln Leu Phe Val Thr Val Val Asp Thr 325 330 335 Thr Arg Ser Thr Asn Met Thr Leu Cys Thr Glu Val Thr Lys Glu Gly 340 345 350 Thr Tyr Lys Asn Asp Asn Phe Lys Glu Tyr Val Arg His Val Glu Glu 355 360 365 Tyr Asp Leu Gln Phe Val Phe Gln Leu Cys Lys Ile Thr Leu Thr Ala 370 375 380 Glu Ile Met Thr Tyr Ile His Thr Met Asp Ser Asn Ile Leu Glu Asp 385 390 395 400 Trp Gln Phe Gly Leu Thr Pro Pro Pro Ser Ala Ser Leu Gln Asp Thr 405 410 415 Tyr Arg Phe Val Thr Ser Gln Ala Ile Thr Cys Gln Lys Thr Ala Pro 420 425 430 Pro Lys Glu Lys Glu Asp Pro Leu Asn Lys Tyr Thr Phe Trp Glu Val 435 440 445 Asn Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro Leu Gly 450 455 460 Arg Lys Phe Leu Leu Gln Ser Gly Leu Lys Ala Lys Pro Lys Leu Lys 465 470 475 480 Arg Ala Ala Pro Thr Ser Thr Arg Thr Ser Ser Ala Lys Arg Lys Lys 485 490 495 Val Lys Lys <![CDATA[<210> 150]]> <![CDATA[<211> 1501]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5804 嵌合的HPV 58型L1蛋白的核苷酸序列]]> <![CDATA[<400> 150]]> atgagcgtgt ggaggcccag cgaggccacc gtgtacctgc cccccgtgcc cgtgagcaag 60 gtggtgagca ccgacgagta cgtgagcagg accagcatct actactacgc cggcagcagc 120 aggctgctgg ccgtgggcaa cccctacttc agcatcaaga gccccaacaa caacaagaag 180 gtgctggtgc ccaaggtgag cggcctgcag tacagggtgt tcagggtgag gctgcccgac 240 cccaacaagt tcggcttccc cgacaccagc ttctacaacc ccgacaccca gaggctggtg 300 tgggcctgcg tgggcctgga gatcggcagg ggccagcccc tgggcgtggg cgtgagcggc 360 cacccctacc tgaacaagtt cgacgacacc gagaccagca acaggtaccc cgcccagccc 420 ggcagcgaca acagggagtg cctgagcatg gactacaagc agacccagct gtgcctgatc 480 ggctgcaagc cccccaccgg cgagcactgg ggcaagggcg tggcctgcaa caacaacgcc 540 gccgccaccg actgcccccc cctggagctg ttcaacagca tcatcgagga cggcgacatg 600 gtggacaccg gcttcggctg catggacttc ggcaccctgc aggccaacaa gagcgacgtg 660 cccatcgaca tctgcaacag cacctgcaag taccccgact acctgaagat ggccagcgag 720 ccctacggcg acagcctgtt cttcttcctg aggagggagc agatgttcgt gaggcacttc 780 ttcaacaggg ccggcaagct gggcgaggcc gtgcccgacg acctgtacat caagggcagc 840 ggcaacaccg ccgtgatcca gagcagcgcc ttcttcccca cccccagcgg cagcatcgtg 900 accagcgaga gccagctgtt caacaagccc tactggctgc agagggccca gggccacaac 960 aacggcatct gctggggcaa ccagctgttc gtgaccgtgg tggacaccac caggagcacc 1020 aacatgaccc tgtgcaccga ggtgaccaag gagggcacct acaagaacga caacttcaag 1080 gagtacgtga ggcacgtgga ggagtacgac ctgcagttcg tgttccagct gtgcaagatc 1140 accctgaccg ccgagatcat gacctacatc cacaccatgg acagcaacat cctggaggac 1200 tggcagttcg gcctgacccc cccccccagc gccagcctgc aggacaccta caggttcgtg 1260 accagccagg ccatcacctg ccagaagacc gcccccccca aggagaagga ggaccccctg 1320 aacaagtaca ccttctggga ggtgaacctg aaggagaagt tcagcgccga cctggaccag 1380 ttccccctgg gcaggaagtt cctgctgcag agcggcctga aagccaagcc aaaactgaaa 1440 agggctgccc caaccagcac caggacctcc tctgccaaga ggaagaaggt gaagaagtaa 1500 a 1501 <![CDATA[<210> 151]]> <![CDATA[<211> 1516]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5805 合成的HPV58L1基因]]> <![CDATA[<400> 151]]> ctgggtacca tgagcgtgtg gaggcccagc gaggccaccg tgtacctgcc ccccgtgccc 60 gtgagcaagg tggtgagcac cgacgagtac gtgagcagga ccagcatcta ctactacgcc 120 ggcagcagca ggctgctggc cgtgggcaac ccctacttca gcatcaagag ccccaacaac 180 aacaagaagg tgctggtgcc caaggtgagc ggcctgcagt acagggtgtt cagggtgagg 240 ctgcccgacc ccaacaagtt cggcttcccc gacaccagct tctacaaccc cgacacccag 300 aggctggtgt gggcctgcgt gggcctggag atcggcaggg gccagcccct gggcgtgggc 360 gtgagcggcc acccctacct gaacaagttc gacgacaccg agaccagcaa caggtacccc 420 gcccagcccg gcagcgacaa cagggagtgc ctgagcatgg actacaagca gacccagctg 480 tgcctgatcg gctgcaagcc ccccaccggc gagcactggg gcaagggcgt ggcctgcaac 540 aacaacgccg ccgccaccga ctgccccccc ctggagctgt tcaacagcat catcgaggac 600 ggcgacatgg tggacaccgg cttcggctgc atggacttcg gcaccctgca ggccaacaag 660 agcgacgtgc ccatcgacat ctgcaacagc acctgcaagt accccgacta cctgaagatg 720 gccagcgagc cctacggcga cagcctgttc ttcttcctga ggagggagca gatgttcgtg 780 aggcacttct tcaacagggc cggcaagctg ggcgaggccg tgcccgacga cctgtacatc 840 aagggcagcg gcaacaccgc cgtgatccag agcagcgcct tcttccccac ccccagcggc 900 agcatcgtga ccagcgagag ccagctgttc aacaagccct actggctgca gagggcccag 960 ggccacaaca acggcatctg ctggggcaac cagctgttcg tgaccgtggt ggacaccacc 1020 aggagcacca acatgaccct gtgcaccgag gtgaccaagg agggcaccta caagaacgac 1080 aacttcaagg agtacgtgag gcacgtggag gagtacgacc tgcagttcgt gttccagctg 1140 tgcaagatca ccctgaccgc cgagatcatg acctacatcc acaccatgga cagcaacatc 1200 ctggaggact ggcagttcgg cctgaccccc ccccccagcg ccagcctgca ggacacctac 1260 aggttcgtga ccagccaggc catcacctgc cagaagaccg ccccccccaa ggagaaggag 1320 gaccccctga acaagtacac cttctgggag gtgaacctga aggagaagtt cagcgccgac 1380 ctggaccagt tccccctggg caggaagttc ctgctgcaga gcggcctgaa ggccaagccc 1440 aggctgaaga ggagcgcccc caccaccagg gcccccagca ccaagaggaa gaaggtgaag 1500 aagtaaactc gagctc 1516 <![CDATA[<210> 152]]> <![CDATA[<211> 1519]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5806 合成的HPV33L1基因]]> <![CDATA[<400> 152]]> ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct 60 gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct 120 ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat 180 gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga 240 ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag 300 agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc 360 atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct 420 ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt 480 tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc 540 aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat 600 ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag 660 tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg 720 acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg 780 agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc 840 aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc 900 agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa 960 ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc 1020 aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag 1080 aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt 1140 tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc 1200 ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac 1260 aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag 1320 gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac 1380 ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca 1440 aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg 1500 aagaagtaaa ctcgagctc 1519 <![CDATA[<210> 153]]> <![CDATA[<211> 34]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5807 HPV58L1 F1]]> <![CDATA[<400> 153]]> cttggtacca tgagcgtgtg gaggcccagc gagg 34 <![CDATA[<210> 154]]> <![CDATA[<211> 36]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5808 HPV58L1 R1]]> <![CDATA[<400> 154]]> gcttggcttt caggccgctc tgcagcagga acttcc 36 <![CDATA[<210> 155]]> <![CDATA[<211> 1438]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5809 HPV58L1擴增序列1]]> <![CDATA[<400> 155]]> cttggtacca tgagcgtgtg gaggcccagc gaggccaccg tgtacctgcc ccccgtgccc 60 gtgagcaagg tggtgagcac cgacgagtac gtgagcagga ccagcatcta ctactacgcc 120 ggcagcagca ggctgctggc cgtgggcaac ccctacttca gcatcaagag ccccaacaac 180 aacaagaagg tgctggtgcc caaggtgagc ggcctgcagt acagggtgtt cagggtgagg 240 ctgcccgacc ccaacaagtt cggcttcccc gacaccagct tctacaaccc cgacacccag 300 aggctggtgt gggcctgcgt gggcctggag atcggcaggg gccagcccct gggcgtgggc 360 gtgagcggcc acccctacct gaacaagttc gacgacaccg agaccagcaa caggtacccc 420 gcccagcccg gcagcgacaa cagggagtgc ctgagcatgg actacaagca gacccagctg 480 tgcctgatcg gctgcaagcc ccccaccggc gagcactggg gcaagggcgt ggcctgcaac 540 aacaacgccg ccgccaccga ctgccccccc ctggagctgt tcaacagcat catcgaggac 600 ggcgacatgg tggacaccgg cttcggctgc atggacttcg gcaccctgca ggccaacaag 660 agcgacgtgc ccatcgacat ctgcaacagc acctgcaagt accccgacta cctgaagatg 720 gccagcgagc cctacggcga cagcctgttc ttcttcctga ggagggagca gatgttcgtg 780 aggcacttct tcaacagggc cggcaagctg ggcgaggccg tgcccgacga cctgtacatc 840 aagggcagcg gcaacaccgc cgtgatccag agcagcgcct tcttccccac ccccagcggc 900 agcatcgtga ccagcgagag ccagctgttc aacaagccct actggctgca gagggcccag 960 ggccacaaca acggcatctg ctggggcaac cagctgttcg tgaccgtggt ggacaccacc 1020 aggagcacca acatgaccct gtgcaccgag gtgaccaagg agggcaccta caagaacgac 1080 aacttcaagg agtacgtgag gcacgtggag gagtacgacc tgcagttcgt gttccagctg 1140 tgcaagatca ccctgaccgc cgagatcatg acctacatcc acaccatgga cagcaacatc 1200 ctggaggact ggcagttcgg cctgaccccc ccccccagcg ccagcctgca ggacacctac 1260 aggttcgtga ccagccaggc catcacctgc cagaagaccg ccccccccaa ggagaaggag 1320 gaccccctga acaagtacac cttctgggag gtgaacctga aggagaagtt cagcgccgac 1380 ctggaccagt tccccctggg caggaagttc ctgctgcaga gcggcctgaa agccaagc 1438 <![CDATA[<210> 156]]> <![CDATA[<211> 35]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5810 HPV58L1 F2]]> <![CDATA[<400> 156]]> gagcggcctg aaagccaagc caaaactgaa aaggg 35 <![CDATA[<210> 157]]> <![CDATA[<211> 36]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5811 HPV58L1 R2]]> <![CDATA[<400> 157]]> ctgtctagat ttacttcttc accttcttcc tcttgg 36 <![CDATA[<210> ]]>158 <![CDATA[<211> 101]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5812 HPV58L1擴增序列2]]> <![CDATA[<400> 158]]> gagcggcctg aaagccaagc caaaactgaa aagggctgcc ccaaccagca ccaggacctc 60 ctctgccaag aggaagaagg tgaagaagta aatctagaca g 101 <![CDATA[<210> 159]]> <![CDATA[<211> 38]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5813 HPV 59型L1蛋白的471-508胺基酸序列]]> <![CDATA[<400> 159]]> Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 1 5 10 15 Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 20 25 30 Arg Lys Ser Ser Arg Lys 35 <![CDATA[<210> 160]]> <![CDATA[<211> 508]]> <![CDATA[<212> PRT]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5901 HPV 59型L1蛋白的胺基酸序列]]> <![CDATA[<400> 160]]> Met Ala Leu Trp Arg Ser Ser Asp Asn Lys Val Tyr Leu Pro Pro Pro 1 5 10 15 Ser Val Ala Lys Val Val Ser Thr Asp Glu Tyr Val Thr Arg Thr Ser 20 25 30 Ile Phe Tyr His Ala Gly Ser Ser Arg Leu Leu Thr Val Gly His Pro 35 40 45 Tyr Phe Lys Val Pro Lys Gly Gly Asn Gly Arg Gln Asp Val Pro Lys 50 55 60 Val Ser Ala Tyr Gln Tyr Arg Val Phe Arg Val Lys Leu Pro Asp Pro 65 70 75 80 Asn Lys Phe Gly Leu Pro Asp Asn Thr Val Tyr Asp Pro Asn Ser Gln 85 90 95 Arg Leu Val Trp Ala Cys Val Gly Val Glu Ile Gly Arg Gly Gln Pro 100 105 110 Leu Gly Val Gly Leu Ser Gly His Pro Leu Tyr Asn Lys Leu Asp Asp 115 120 125 Thr Glu Asn Ser His Val Ala Ser Ala Val Asp Thr Lys Asp Thr Arg 130 135 140 Asp Asn Val Ser Val Asp Tyr Lys Gln Thr Gln Leu Cys Ile Ile Gly 145 150 155 160 Cys Val Pro Ala Ile Gly Glu His Trp Thr Lys Gly Thr Ala Cys Lys 165 170 175 Pro Thr Thr Val Val Gln Gly Asp Cys Pro Pro Leu Glu Leu Ile Asn 180 185 190 Thr Pro Ile Glu Asp Gly Asp Met Val Asp Thr Gly Tyr Gly Ala Met 195 200 205 Asp Phe Lys Leu Leu Gln Asp Asn Lys Ser Glu Val Pro Leu Asp Ile 210 215 220 Cys Gln Ser Ile Cys Lys Tyr Pro Asp Tyr Leu Gln Met Ser Ala Asp 225 230 235 240 Ala Tyr Gly Asp Ser Met Phe Phe Cys Leu Arg Arg Glu Gln Val Phe 245 250 255 Ala Arg His Phe Trp Asn Arg Ser Gly Thr Met Gly Asp Gln Leu Pro 260 265 270 Glu Ser Leu Tyr Ile Lys Gly Thr Asp Ile Arg Ala Asn Pro Gly Ser 275 280 285 Tyr Leu Tyr Ser Pro Ser Pro Ser Gly Ser Val Val Thr Ser Asp Ser 290 295 300 Gln Leu Phe Asn Lys Pro Tyr Trp Leu His Lys Ala Gln Gly Leu Asn 305 310 315 320 Asn Gly Ile Cys Trp His Asn Gln Leu Phe Leu Thr Val Val Asp Thr 325 330 335 Thr Arg Ser Thr Asn Leu Ser Val Cys Ala Ser Thr Thr Ser Ser Ile 340 345 350 Pro Asn Val Tyr Thr Pro Thr Ser Phe Lys Glu Tyr Ala Arg His Val 355 360 365 Glu Glu Phe Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu 370 375 380 Thr Thr Glu Val Met Ser Tyr Ile His Asn Met Asn Thr Thr Ile Leu 385 390 395 400 Glu Asp Trp Asn Phe Gly Val Thr Pro Pro Pro Thr Ala Ser Leu Val 405 410 415 Asp Thr Tyr Arg Phe Val Gln Ser Ala Ala Val Thr Cys Gln Lys Asp 420 425 430 Thr Ala Pro Pro Val Lys Gln Asp Pro Tyr Asp Lys Leu Lys Phe Trp 435 440 445 Pro Val Asp Leu Lys Glu Arg Phe Ser Ala Asp Leu Asp Gln Phe Pro 450 455 460 Leu Gly Arg Lys Phe Leu Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr 465 470 475 480 Ile Gly Pro Arg Lys Arg Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser 485 490 495 Pro Lys Arg Val Lys Arg Arg Lys Ser Ser Arg Lys 500 505 <![CDATA[<210> 161]]> <![CDATA[<211> 1528]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 智人]]> <![CDATA[<220> ]]> <![CDATA[<223> 5902 HPV 59型L1蛋白的核苷酸序列]]> <![CDATA[<400> 161]]> atggctctgt ggaggtcctc tgacaacaag gtctacctgc ctcctccatc tgtggctaag 60 gtggtgagca cagatgaata tgtgaccagg accagcatct tctaccatgc tggctccagc 120 agactgctga cagtgggaca cccatacttc aaggtgccaa agggaggcaa tggcagacag 180 gatgtgccaa aggtgtctgc ctaccaatac agggtgttca gggtgaaact gcctgaccca 240 aacaagtttg gactgcctga caacacagtc tatgacccaa acagccagag actggtgtgg 300 gcttgtgtgg gagtggagat tggcagggga caaccactgg gagtgggact gtctggacac 360 ccactctaca acaaactgga tgacacagag aactctcatg tggcatctgc tgtggacacc 420 aaggacacca gggacaatgt gtctgtggac tacaagcaga cccaactttg tatcattggc 480 tgtgtgcctg ccattggaga acactggacc aagggcacag cctgtaagcc aaccacagtg 540 gtccagggag actgtcctcc attggaactg ataaacacac caattgagga tggagatatg 600 gtggacacag gctatggagc tatggacttc aaactgctcc aagacaacaa gtctgaggtg 660 ccactggaca tctgtcagag catctgtaaa taccctgact acctccaaat gagtgctgat 720 gcctatggag acagtatgtt cttctgtctg aggagggaac aggtgtttgc cagacacttc 780 tggaacaggt ctggcacaat gggagaccaa cttcctgagt ccctctacat caagggcaca 840 gacatcaggg ctaaccctgg ctcctacctc tacagcccaa gcccatctgg ctctgtggtg 900 acctctgaca gccaactttt caacaagcca tactggctgc acaaggctca aggactgaac 960 aatggcatct gttggcacaa ccaacttttc ctgacagtgg tggacaccac caggagcacc 1020 aacctgtctg tgtgtgccag caccacctcc agcatcccaa atgtctacac accaacctcc 1080 ttcaaggaat atgccaggca tgtggaggag tttgacctcc aattcatctt ccaactttgt 1140 aagattaccc tgaccacaga ggtgatgagt tacatccaca atatgaacac caccatcttg 1200 gaggactgga actttggagt gacacctcct ccaacagcct ccctggtgga cacctacagg 1260 tttgtccagt ctgctgctgt gacttgtcag aaggacacag cccctcctgt gaagcaggac 1320 ccatatgaca aactgaagtt ctggcctgtg gacctgaaag agaggttctc tgctgacctg 1380 gaccagtttc cactgggcag gaagttcctg ctccaacttg gagccagacc aaagccaacc 1440 attggaccaa ggaagagggc tgcccctgcc ccaaccagca caccaagccc aaagagggtg 1500 aagaggagga agtccagcag gaagtaaa 1528 <![CDATA[<210> 162]]> <![CDATA[<211> 1546]]> <![CDATA[<212> DNA]]> <![CDATA[<213> 人工序列]]> <![CDATA[<220> ]]> <![CDATA[<223> 5903 合成的HPV59L1基因]]> <![CDATA[<400> 162]]> ctgggtacca tggctctgtg gaggtcctct gacaacaagg tctacctgcc tcctccatct 60 gtggctaagg tggtgagcac agatgaatat gtgaccagga ccagcatctt ctaccatgct 120 ggctccagca gactgctgac agtgggacac ccatacttca aggtgccaaa gggaggcaat 180 ggcagacagg atgtgccaaa ggtgtctgcc taccaataca gggtgttcag ggtgaaactg 240 cctgacccaa acaagtttgg actgcctgac aacacagtct atgacccaaa cagccagaga 300 ctggtgtggg cttgtgtggg agtggagatt ggcaggggac aaccactggg agtgggactg 360 tctggacacc cactctacaa caaactggat gacacagaga actctcatgt ggcatctgct 420 gtggacacca aggacaccag ggacaatgtg tctgtggact acaagcagac ccaactttgt 480 atcattggct gtgtgcctgc cattggagaa cactggacca agggcacagc ctgtaagcca 540 accacagtgg tccagggaga ctgtcctcca ttggaactga taaacacacc aattgaggat 600 ggagatatgg tggacacagg ctatggagct atggacttca aactgctcca agacaacaag 660 tctgaggtgc cactggacat ctgtcagagc atctgtaaat accctgacta cctccaaatg 720 agtgctgatg cctatggaga cagtatgttc ttctgtctga ggagggaaca ggtgtttgcc 780 agacacttct ggaacaggtc tggcacaatg ggagaccaac ttcctgagtc cctctacatc 840 aagggcacag acatcagggc taaccctggc tcctacctct acagcccaag cccatctggc 900 tctgtggtga cctctgacag ccaacttttc aacaagccat actggctgca caaggctcaa 960 ggactgaaca atggcatctg ttggcacaac caacttttcc tgacagtggt ggacaccacc 1020 aggagcacca acctgtctgt gtgtgccagc accacctcca gcatcccaaa tgtctacaca 1080 ccaacctcct tcaaggaata tgccaggcat gtggaggagt ttgacctcca attcatcttc 1140 caactttgta agattaccct gaccacagag gtgatgagtt acatccacaa tatgaacacc 1200 accatcttgg aggactggaa ctttggagtg acacctcctc caacagcctc cctggtggac 1260 acctacaggt ttgtccagtc tgctgctgtg acttgtcaga aggacacagc ccctcctgtg 1320 aagcaggacc catatgacaa actgaagttc tggcctgtgg acctgaaaga gaggttctct 1380 gctgacctgg accagtttcc actgggcagg aagttcctgc tccaacttgg agccagacca 1440 aagccaacca ttggaccaag gaagagggct gcccctgccc caaccagcac accaagccca 1500 aagagggtga agaggaggaa gtccagcagg aagtaaactc gagctc 1546
Claims (24)
- 一種嵌合的乳頭瘤病毒L1蛋白,自其N末端至C末端方向包含: a. 衍生於第一型別乳頭瘤病毒L1蛋白的N端片段,所述N端片段保持第一型別乳頭瘤病毒L1蛋白的免疫原性;和 b. 衍生於第二型別乳頭瘤病毒L1蛋白的C端片段,所述第二型別乳頭狀瘤病毒L1蛋白具有相較於其他型別的L1蛋白表達量和可溶性較好的特性; 其中所述嵌合的乳頭瘤病毒L1蛋白具有第一型別乳頭瘤病毒L1蛋白的免疫原性。
- 如請求項1所述的嵌合的乳頭瘤病毒L1蛋白,其中 所述N端片段為將所述第一型別乳頭瘤病毒L1蛋白的天然序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段,以及與其具有至少98%的同一性的片段;並且 所述C端片段為將第二型別乳頭狀瘤病毒L1蛋白的天然序列的N末端截短於其α5區內的任一胺基酸位點而得到的片段,以及該片段進一步突變、缺失和/或添加而產生的功能性變體。
- 如請求項2所述的嵌合的乳頭瘤病毒L1蛋白,其中所述C端片段含有一個或多個核定位序列。
- 如請求項1所述的嵌合的乳頭瘤病毒L1蛋白,其中所述乳頭瘤病毒L1蛋白是HPV L1蛋白。
- 如請求項1所述的嵌合的乳頭瘤病毒L1蛋白,其中所述第二型別乳頭瘤病毒L1蛋白選自HPV 1型、2型、3型、4型、6型、7型、10型、11型、13型、16型、18型、22型、26型、28型、31型、32型、33型、35型、39型、42型、44型、45型、51型、52型、53型、56型、58型、59型、60型、63型、66型、68型、73型或82型L1蛋白; 較佳地,所述第二型別乳頭瘤病毒L1蛋白選自HPV 16型、28型、33型、59型、或68型L1蛋白; 更佳地,所述第二型別乳頭瘤病毒L1蛋白選自HPV 33型或HPV 59型L1蛋白。
- 如請求項5所述的嵌合的乳頭瘤病毒L1蛋白,其中所述C端片段為SEQ ID No: 2;或其長度為m1個胺基酸的片段,較佳涵蓋SEQ ID No: 2的第1-m1位胺基酸的片段;其中m1為8-26的整數;或所述C端片段為SEQ ID No: 135;或其長度為m2個胺基酸的片段,較佳涵蓋SEQ ID No: 135的第1-m2位胺基酸的片段;其中m2為13-31的整數。
- 如請求項5所述的嵌合的乳頭瘤病毒L1蛋白,其中所述C端片段為SEQ ID No: 13;或其長度為n個胺基酸的片段,較佳涵蓋SEQ ID No: 13的第1-n 位胺基酸的片段;其中n為16-38的整數。
- 如請求項1所述的嵌合的乳頭瘤病毒L1蛋白,其中所述第一型別乳頭瘤病毒L1蛋白選自HPV 6型、11型、16型、18型、31型、35型、39型、45型、51型、52型、56型或58型L1蛋白。
- 如請求項1所述的嵌合的乳頭瘤病毒L1蛋白,其中 所述HPV 6型L1蛋白的N端片段與將SEQ ID No: 1所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性; 所述HPV 11型L1蛋白的N端片段與將SEQ ID No: 14所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性; 所述HPV 16型L1蛋白的N端片段與將SEQ ID No: 27所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性; 所述HPV 18型L1蛋白的N端片段與將SEQ ID No: 40所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性; 所述HPV 31型L1蛋白的N端片段與將SEQ ID No: 53所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性; 所述HPV 35型L1蛋白的N端片段與將SEQ ID No: 69所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性; 所述HPV 39型L1蛋白的N端片段與將SEQ ID No: 82所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性; 所述HPV 45型L1蛋白的N端片段與將SEQ ID No: 95所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性; 所述HPV 51型L1蛋白的N端片段與將SEQ ID No: 108所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性; 所述HPV 52型L1蛋白的N端片段與將SEQ ID No: 121所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性; 所述HPV 56型L1蛋白的N端片段與將SEQ ID No: 134所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性;和 所述HPV 58型L1蛋白的N端片段與將SEQ ID No: 147所示序列的C末端截短於其α5區內的任一胺基酸位點而得到的片段具有98%、98.5%、99%、99.5%、99%或100%的同一性。
- 如請求項1-9中任一項所述的嵌合的乳頭瘤病毒L1蛋白,所述N端片段的C末端與所述C端片段的N末端直接連接或透過連接子連接。
- 如請求項1-10中任一項所述的嵌合的乳頭瘤病毒L1蛋白,其中當所述N端片段的C末端與所述C端片段的N末端連接時,在連接點的正負4個胺基酸位點的範圍內存在以下連續胺基酸序列:RKFL; 較佳地,在連接點的正負6個胺基酸位點的範圍內存在以下連續胺基酸序列:LGRKFL。
- 如請求項11 所述的嵌合的乳頭瘤病毒L1蛋白,所述嵌合的HPV 6型、11型、16型、18型、31型、35型、39型、45型、51型、52型、56型和58型嵌合HPV L1蛋白分別與SEQ ID No: 3、SEQ ID No: 16、SEQ ID No: 29、SEQ ID No: 42、SEQ ID No: 55、SEQ ID No: 71、SEQ ID No: 84、SEQ ID No: 97、SEQ ID No: 110、SEQ ID No: 123、SEQ ID No: 136和SEQ ID No: 149具有98%、98.5%、99%、99.5%或100%的同一性。
- 一種乳頭瘤類病毒顆粒,其包含如請求項1至12中任一項所述的嵌合的乳頭瘤病毒L1蛋白。
- 一種分離的多核苷酸,其編碼如請求項1至13中任一項所述的嵌合的乳頭瘤病毒L1蛋白。
- 一種載體,其包含如請求項14所述的多核苷酸; 較佳地,為桿狀病毒載體。
- 一種桿狀病毒,其包含如請求項16所述的多核苷酸。
- 一種宿主細胞,其包含如請求項14所述的多核苷酸、如請求項15所述的載體、或如請求項16所述的桿狀病毒; 較佳地,其為昆蟲細胞,較佳地,所述昆蟲細胞選自Sf9細胞、Sf21細胞、Hi5細胞和S2細胞。
- 一種預防HPV相關疾病或感染的多價HPV免疫原性組合物,其包含如請求項13 所述的HPV類病毒顆粒、如請求項14 所述的多核苷酸、如請求項15所述的載體、如請求項16 所述的桿狀病毒、如請求項17所述的細胞。
- 如請求項18的HPV免疫原性組合物,其還包含生理學上可接受的載體以及 任選地,還包含佐劑。
- 如請求項19所述的多價HPV免疫原性組合物,其中所述佐劑為磷酸鋁佐劑。
- 如請求項18-20所述的HPV免疫原性組合物,其包含如請求項13所述的類病毒顆粒,以及一種或多種由其他致病的HPV型別的L1蛋白組裝而成的HPV類病毒顆粒。
- 如請求項21的HPV免疫原性組合物,其包含 HPV 6型、11型、16型、18型、31型、35型、39型、45型、51型、52型、56型和58型類病毒顆粒,組成類病毒顆粒的嵌合HPV L1蛋白分別與SEQ ID No: 3、SEQ ID No: 16、SEQ ID No: 29、SEQ ID No: 42、SEQ ID No: 55、SEQ ID No: 71、SEQ ID No: 84、SEQ ID No: 97、SEQ ID No: 110、SEQ ID No: 123、SEQ ID No: 136和SEQ ID No: 149具有98%、98.5%、99%、99.5%或100%的同一性;以及 HPV 33型和HPV 59型類病毒顆粒,其中HPV 33型L1蛋白和HPV 59型L1蛋白分別與SEQ ID No: 66和SEQ ID No: 160具有98%、98.5%、99%、99.5%或100%的同一性。
- 一種預防HPV相關疾病或感染的方法,其包括: 向受試者施用如請求項13-22中任一項所述的HPV免疫原性組合物。
- 一種如請求項1-12所述的嵌合的乳頭瘤病毒L1蛋白、如請求項13 所述的HPV類病毒顆粒、如請求項14 所述的多核苷酸、如請求項15或16 所述的載體、如請求項17 所述的桿狀病毒、如請求項18或19所述的細胞、如請求項18-22中任一項所述的免疫原性組合物在用於製備用於預防HPV相關疾病或感染的疫苗或藥物中的用途。
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